Base de dados : MEDLINE
Pesquisa : D12.776.395.560.631.100.750 [Categoria DeCS]
Referências encontradas : 239 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 24 ir para página                         

  1 / 239 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28685935
[Au] Autor:Ohya A; Yamanoi K; Shimojo H; Fujii C; Nakayama J
[Ad] Endereço:Department of Molecular Pathology, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
[Ti] Título:Gastric gland mucin-specific O-glycan expression decreases with tumor progression from precursor lesions to pancreatic cancer.
[So] Source:Cancer Sci;108(9):1897-1902, 2017 Sep.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pancreatic cancer is lethal, as it is often detected late. Thus, novel biomarkers of precursor lesions are needed to devise timely therapies. Pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN) are major precursors of pancreatic cancer. In normal gastric mucosa, gastric gland mucin-specific O-glycans are unique in having α1,4-linked N-acetylglucosamine (αGlcNAc) residues attached to MUC6. Recently we reported that αGlcNAc functions as a tumor suppressor for differentiated-type gastric adenocarcinoma (Karasawa et al., J Clin Invest 122, 923, 2012). MUC6 is also expressed in pancreatic neoplasms, including PanIN and IPMN, but the role of αGlcNAc expression in pancreatic neoplasms remains unknown. Here, we analyze expression patterns of αGlcNAc, MUC6 and MUC5AC in pancreatic neoplasms and compare them with progression from PanIN to invasive ductal adenocarcinoma (IDAC) (the PanIN-IDAC sequence; 20 cases) and from IPMN to IPMN with associated invasive carcinoma (IPMNAIC) (the IPMN-IPMNAIC sequence; 20 cases). At both sequences, the frequency of MUC6-positive and αGlcNAc-positive lesions decreased with tumor progression. We then compared expression levels of αGlcNAc and MUC6 at each step of the progression. At the PanIN-IDAC sequence, αGlcNAc expression significantly decreased relative to MUC6 in low-grade PanIN (P = 0.021), high-grade PanIN/intraductal spread of IDAC (P = 0.031) and IDAC (P = 0.013). At the IPMN-IPMNAIC sequence, decreased αGlcNAc expression was also observed in low-grade IPMN exhibiting gastric-type morphology (P = 0.020). These results suggest that decreased expression of αGlcNAc relative to MUC6 occurs early and marks the initiation of tumor progression to pancreatic cancer.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Carcinoma Ductal Pancreático/metabolismo
Mucosa Gástrica/metabolismo
Mucina-6/metabolismo
Neoplasias Pancreáticas/metabolismo
[Mh] Termos MeSH secundário: Acetilglucosamina/metabolismo
Configuração de Carboidratos
Carcinogênese
Carcinoma Ductal Pancreático/patologia
Progressão da Doença
Glicosilação
Seres Humanos
Mucina-5AC/metabolismo
Neoplasias Pancreáticas/patologia
Lesões Pré-Cancerosas
Processamento de Proteína Pós-Traducional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (MUC5AC protein, human); 0 (MUC6 protein, human); 0 (Mucin 5AC); 0 (Mucin-6); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13317


  2 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28505002
[Au] Autor:Xue Y; Reid MD; Balci S; Quigley B; Muraki T; Memis B; Xia J; Hacihasanoglu E; Bedolla G; Pehlivanoglu B; Kim GE; Tajiri T; Ohike N; Aneja R; Krasinskas AM; Adsay V
[Ad] Endereço:*Department of Pathology, Emory University School of Medicine Departments of †Mathematics and Statistics ¶Biology, Georgia State University, Atlanta, GA ‡Department of Pathology, University of California San Francisco, San Francisco, CA §Department of Pathology, Tokai University Hachioji Hospital, Tokyo ∥Department of Pathology, Showa University Fujigaoka Hospital, Yokohama, Japan.
[Ti] Título:Immunohistochemical Classification of Ampullary Carcinomas: Critical Reappraisal Fails to Confirm Prognostic Relevance for Recently Proposed Panels, and Highlights MUC5AC as a Strong Prognosticator.
[So] Source:Am J Surg Pathol;41(7):865-876, 2017 Jul.
[Is] ISSN:1532-0979
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recently, immunohistochemistry-based classifications of ampullary carcinomas have been proposed (Ang and colleagues [PMID: 24832159]; Chang and colleagues [PMID: 23439753]). In this study, the prognostic value of Ang/Chang panel markers (CK20, MUC1, MUC2, CDX2) as well as other markers (CK7, MUC5AC, and MUC6) were tested on full-faced sections of 136 ampullary carcinoma resections with substantial (>5 mm) invasion. Immunohistochemistry was correlated with both histologic classification (intestinal [INT], pancreatobiliary [PB], or nontubular based on ≥3/5 observer agreement) and clinical outcome. No prognostic correlation was found with MUC1, CDX2, MUC2 or CK20 despite testing with different quantitative cutoffs. CK7 and CK20 were nonspecific. Ang classification had reasonable correlation with histologic subclassification of tubular cases as INT versus PB with high specificity but low sensitivity and ambiguous category was large (29%) and included also some classical cases. Prognostically, Ang classification approached but did not reach statistical significance, even when their large "ambiguous" group was eliminated and only tubular cases were analyzed (Ang-INT vs. Ang-PB; P=0.08). The Chang panel, in which the definition of the INT subcategory is not clearly defined, only marginally reached prognostic significance when tested as MUC1+/CDX2- versus MUC1-/CDX2+ and only by Wilcoxon test (P=0.0485) but 31% of the cases were "unclassifiable." The only individual marker that was found to have direct and strong correlation with the clinical outcome was MUC5AC (not used in the Ang or Chang panels), with statistically significant survival differences found with various cutoffs tested (for 20% cutoff, 5-y survival, 68% vs. 31%; P=0.0002). In addition, MUC5AC significantly stratified the histologically PB and INT cases (P=0.01 and 0.03, respectively), as well as Ang's ambiguous and Chang's unclassified cases (P=0.006 and 0.007, respectively). In conclusion, the widely used putative lineage markers, MUC1/MUC2/CK7/CK20/CDX2, do not seem to have direct/significant prognostic correlation either individually or in combination of Ang and Chang panels. Ang panel is helpful as an adjunct in determining the cell lineage with a few caveats. MUC5AC proves to be a significant independent prognosticator and should be incorporated into evaluation of ampullary carcinomas.
[Mh] Termos MeSH primário: Adenocarcinoma/diagnóstico
Ampola Hepatopancreática/metabolismo
Biomarcadores Tumorais/metabolismo
Neoplasias do Ducto Colédoco/diagnóstico
Mucina-5AC/metabolismo
[Mh] Termos MeSH secundário: Adenocarcinoma/metabolismo
Adenocarcinoma/mortalidade
Adenocarcinoma/patologia
Adulto
Idoso
Idoso de 80 Anos ou mais
Ampola Hepatopancreática/patologia
Neoplasias do Ducto Colédoco/metabolismo
Neoplasias do Ducto Colédoco/mortalidade
Neoplasias do Ducto Colédoco/patologia
Feminino
Seguimentos
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Mucina-6/metabolismo
Prognóstico
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (MUC5AC protein, human); 0 (MUC6 protein, human); 0 (Mucin 5AC); 0 (Mucin-6)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1097/PAS.0000000000000863


  3 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27563024
[Au] Autor:Yoo KS; Choi HS; Jun DW; Lee HL; Lee OY; Yoon BC; Lee KG; Paik SS; Kim YS; Lee J
[Ad] Endereço:Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
[Ti] Título:MUC Expression in Gallbladder Epithelial Tissues in Cholesterol-Associated Gallbladder Disease.
[So] Source:Gut Liver;10(5):851-8, 2016 Sep 15.
[Is] ISSN:2005-1212
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterol-associated gallbladder disease and evaluated the relationship between mucin and bacterial infection. METHODS: The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. RESULTS: MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. CONCLUSIONS: The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression.
[Mh] Termos MeSH primário: Colecistite/metabolismo
Células Epiteliais/metabolismo
Doenças da Vesícula Biliar/metabolismo
Mucinas Gástricas/metabolismo
Hipercolesterolemia/metabolismo
[Mh] Termos MeSH secundário: Animais
Estudos de Casos e Controles
Colecistite/etiologia
Cães
Vesícula Biliar/citologia
Doenças da Vesícula Biliar/etiologia
Seres Humanos
Hipercolesterolemia/complicações
Mucina-5AC/metabolismo
Mucina-3/metabolismo
Mucina-5B/metabolismo
Mucina-6/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gastric Mucins); 0 (Mucin 5AC); 0 (Mucin-3); 0 (Mucin-5B); 0 (Mucin-6)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160827
[St] Status:MEDLINE
[do] DOI:10.5009/gnl15600


  4 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27523981
[Au] Autor:Yamaguchi J; Mino-Kenudson M; Liss AS; Chowdhury S; Wang TC; Fernández-Del Castillo C; Lillemoe KD; Warshaw AL; Thayer SP
[Ad] Endereço:Department of Surgery, Andrew L. Warshaw Institute for Pancreatic Cancer Research, Massachusetts General Hospital, Boston, Massachusetts.
[Ti] Título:Loss of Trefoil Factor 2 From Pancreatic Duct Glands Promotes Formation of Intraductal Papillary Mucinous Neoplasms in Mice.
[So] Source:Gastroenterology;151(6):1232-1244.e10, 2016 Dec.
[Is] ISSN:1528-0012
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND & AIMS: Little is known about the origin of pancreatic intraductal papillary mucinous neoplasms (IPMN). Pancreatic duct glands (PDGs) are gland-like outpouches budding off the main pancreatic ducts that function as a progenitor niche for the ductal epithelium; they express gastric mucins and have characteristics of side-branch IPMNs. We investigated whether PDGs are a precursor compartment for IPMNs and the role of Trefoil factor family 2 (TFF2)-a protein expressed by PDGs and the gastric mucosa that are involved in epithelial repair and tumor suppression. METHODS: We obtained pancreatectomy specimens from 20 patients with chronic pancreatitis, 13 with low-grade side-branch IPMNs, and 15 patients with PDAC; histologically normal pancreata were used as controls (n = 18). Samples were analyzed by immunohistochemistry to detect TFF1 and TFF2 and cell proliferation. We performed mitochondrial DNA mutational mapping studies to determine the cell lineage and fate of PDG cells. Pdx1-Cre;LSL-KRAS (KC) mice were bred with TFF2-knockout mice to generate KC/Tff2 and KC/Tff2 mice. Pancreata were collected and histologically analyzed for formation of IPMN, pancreatic intraepithelial neoplasias, and PDAC, in addition to proliferation and protein expression. Human pancreatic ductal epithelial cells and PDAC cell lines were transfected with vectors to overexpress or knock down TFF2 or SMAD4. RESULTS: Histologic analysis of human samples revealed gastric-type IPMN to comprise 2 molecularly distinct layers: a basal crypt segment that expressed TFF2 and overlying papillary projections. Proliferation occurred predominantly in the PDG-containing basal segments. Mitochondrial mutation mapping revealed a 97% match between the profiles of proliferating PDG cells and their overlying nonproliferative IPMN cells. In contrast to KC mice, 2-month-old KC/Tff2 and KC/Tff2 mice developed prominent papillary structures in the duct epithelium with cystic metaplasia of the PDG, which resembled human IPMN; these expressed gastric mucins (MUC5AC and MUC6), but not the intestinal mucin MUC2. KC/TFF2-knockout mice developed a greater number and higher grade of pancreatic intraepithelial neoplasias than KC mice, and 1 mouse developed an invasive adenocarcinoma. Expression of TFF2 reduced proliferation of PDAC cells 3-fold; this effect required up-regulation and activation of SMAD4. We found expression of TFF2 to be down-regulated in human PDAC by hypermethylation of its promoter. CONCLUSIONS: In histologic analyses of human IPMNs, we found PDGs to form the basal segment and possibly serve as a progenitor compartment. TFF2 has tumor-suppressor activity in the mouse pancreas and prevents formation of mucinous neoplasms.
[Mh] Termos MeSH primário: Epitélio/patologia
Neoplasias Císticas, Mucinosas e Serosas/patologia
Ductos Pancreáticos/patologia
Neoplasias Pancreáticas/patologia
Fator Trefoil-2/genética
Fator Trefoil-2/metabolismo
[Mh] Termos MeSH secundário: Animais
Carcinoma Ductal Pancreático
Linhagem Celular Tumoral
Proliferação Celular/genética
Metilação de DNA
Análise Mutacional de DNA
DNA Mitocondrial/análise
Regulação para Baixo
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Antígeno Ki-67/análise
Masculino
Metaplasia/patologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Mucina-5AC/análise
Mucina-6/análise
Mutação
Neoplasias Císticas, Mucinosas e Serosas/química
Neoplasias Císticas, Mucinosas e Serosas/genética
Pâncreas
Ductos Pancreáticos/química
Neoplasias Pancreáticas/química
Neoplasias Pancreáticas/genética
Pancreatite Crônica
Regiões Promotoras Genéticas/genética
Proteína Smad4/genética
Proteína Smad4/metabolismo
Fator Trefoil-1/análise
Fator Trefoil-2/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Ki-67 Antigen); 0 (Muc5ac protein, mouse); 0 (Muc6 protein, mouse); 0 (Mucin 5AC); 0 (Mucin-6); 0 (Smad4 Protein); 0 (Smad4 protein, mouse); 0 (TFF2 protein, human); 0 (TFF2 protein, mouse); 0 (Tff1 protein, mouse); 0 (Trefoil Factor-1); 0 (Trefoil Factor-2)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE


  5 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27387375
[Au] Autor:Simpson HV; Umair S; Hoang VC; Savoian MS
[Ad] Endereço:Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand. Electronic address: H.V.Simpson@massey.ac.nz.
[Ti] Título:Histochemical study of the effects on abomasal mucins of Haemonchus contortus or Teladorsagia circumcincta infection in lambs.
[So] Source:Vet Parasitol;226:210-21, 2016 Aug 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Previously, chemical analysis of gastric fundic mucin showed that infection of sheep with Haemonchus contortus or Teladorsagia circumcincta changed the proportions of monosaccharides and decreased terminal mucin fucosylation and sialylation. To identify the effects of these parasites on the two mucin-secreting cell lineages, fundic and antral tissues were collected for histochemistry from 69 lambs aged from 3-4 to 9-10 months-of-age which had received a single infection of either H. contortus or T. circumcincta and euthanased at Day 21 or 28 post- infection respectively. All fundic tissues were stained separately with: (1) with Periodic Acid Schiff (PAS) for all mucins; (2) Alcian Blue (AB) pH 2.5 for acidic mucins (sialylated and sulphated); (3) AB pH 1 for sulphated mucins and (4) High Iron Diamine (HID) for sulphated mucins. Antral and fundic tissues from 24 lambs were also stained for acidic and neutral mucins or with specific lectins for α-1-linked fucose and for α-2,3- and α-2,6-linked sialic acids. Only mucin sulphation appeared to differ visually in uninfected lambs over this age range: there was weak staining with HID in tissues from lambs 3-6 months-of-age, but was generally more intense in those over 7 months-of-age. Sulphomucins were not apparent in surface mucous cells (SMC) or generally in the upper pits. Sialylomucins were located predominantly in the pits and glands, with small amounts of sialylated mucins in SMC and on the luminal surface, mainly in younger animals up to 6 months-of-age and less in the older animals. Parasitism markedly reduced the predominantly neutral surface mucin5AC of the SMC and pit cells, despite pit elongation in both antrum and fundus, whereas the acidic Muc6 secreted by mucus neck cells (MNC) increased along with MNC hyperplasia. Sulphated mucins were present mainly from the mid-pits downward and heavy staining was more common in older animals. In these sheep, the markedly reduced neutral mucin in the SMC and pit cells in both antrum and fundus contrasts with reported hypersecretion of mucus in the intestine, which is believed to aid in parasite expulsion. It has been proposed that intestinal goblet cell hypersecretion occurs only in resistant animals, therefore reduced mucins in the abomasum may be indicative of susceptibility to abomasal parasites.
[Mh] Termos MeSH primário: Abomaso/metabolismo
Haemonchus/metabolismo
Mucinas/metabolismo
Doenças dos Ovinos/metabolismo
Trichostrongyloidea/metabolismo
Tricostrongiloidíase/veterinária
[Mh] Termos MeSH secundário: Abomaso/parasitologia
Abomaso/patologia
Fatores Etários
Animais
Fezes/parasitologia
Fundo Gástrico/metabolismo
Fundo Gástrico/parasitologia
Fundo Gástrico/patologia
Glicosilação
Hemoncose/metabolismo
Hemoncose/veterinária
Lectinas/metabolismo
Masculino
Mucina-5AC/metabolismo
Mucina-6/metabolismo
Mucinas/classificação
Naftoquinonas
Antro Pilórico/metabolismo
Antro Pilórico/parasitologia
Antro Pilórico/patologia
Ovinos
Doenças dos Ovinos/parasitologia
Tricostrongiloidíase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lectins); 0 (Mucin 5AC); 0 (Mucin-6); 0 (Mucins); 0 (Naphthoquinones); 0 (histochrome)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE


  6 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27298226
[Au] Autor:Betge J; Schneider NI; Harbaum L; Pollheimer MJ; Lindtner RA; Kornprat P; Ebert MP; Langner C
[Ad] Endereço:Department of Medicine II, University Hospital Mannheim, Medical Faculty Mannheim: Heidelberg University, Mannheim, Germany.
[Ti] Título:MUC1, MUC2, MUC5AC, and MUC6 in colorectal cancer: expression profiles and clinical significance.
[So] Source:Virchows Arch;469(3):255-65, 2016 Sep.
[Is] ISSN:1432-2307
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Mucin glycoprotein expression can be altered during the carcinogenic process. The impact on the prognosis of patients with colorectal cancer (CRC) is controversial. We analyzed tumors from 381 patients for MUC1, MUC2, MUC5AC, and MUC6 expression by immunohistochemical staining, using tissue microarrays. Progression-free and cancer-specific survival were determined using the Kaplan-Meier method. Expression of intestinal mucin MUC2 was lost in 85 (23 %) CRCs, and patients with MUC6-negative tumors showed shorter progression-free survival (PFS, p = 0.043). Gastric mucins MUC5AC and MUC6 showed high (>50 %) aberrant expression in 28 (8 %) and 9 (2 %) cases, respectively. High expression of MUC5AC was associated with longer PFS (p = 0.055). High expression of MUC6 was associated with 100 % PFS (p = 0.024) and longer cancer-specific survival (CSS, p = 0.043). MUC1 was expressed in 238 (64 %) tumors and had no impact on outcome. When analysis was restricted to stages II and III, loss of MUC2 was associated with adverse outcome. Overexpression of both MUC5AC and MUC6 significantly predicted favorable PFS and CSS. In conclusion, loss of MUC2 expression proved to be a predictor of adverse outcome, while the gain of aberrant expression of MUC5AC and particularly of MUC6 was associated with favorable outcome in CRC, notably in intermediate stages II and III.
[Mh] Termos MeSH primário: Antígenos de Neoplasias/metabolismo
Neoplasias Colorretais/diagnóstico
Neoplasias Colorretais/metabolismo
Mucina-5AC/metabolismo
Mucina-2/metabolismo
Mucina-6/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais/análise
Neoplasias Colorretais/patologia
Feminino
Seres Humanos
Imuno-Histoquímica/métodos
Masculino
Meia-Idade
Mucina-1/metabolismo
Prognóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (Biomarkers, Tumor); 0 (MUC2 protein, human); 0 (MUC6 protein, human); 0 (Mucin 5AC); 0 (Mucin-1); 0 (Mucin-2); 0 (Mucin-6)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160615
[St] Status:MEDLINE
[do] DOI:10.1007/s00428-016-1970-5


  7 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27189557
[Au] Autor:Lang T; Klasson S; Larsson E; Johansson ME; Hansson GC; Samuelsson T
[Ad] Endereço:Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan, China.
[Ti] Título:Searching the Evolutionary Origin of Epithelial Mucus Protein Components-Mucins and FCGBP.
[So] Source:Mol Biol Evol;33(8):1921-36, 2016 Aug.
[Is] ISSN:1537-1719
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gel-forming mucins are large glycosylated proteins that are essential components of the mucus layers covering epithelial cells. Using novel methods of identifying mucins based on profile hidden Markov models, we have found a large number of such proteins in Metazoa, aiding in their classification and allowing evolutionary studies. Most vertebrates have 5-6 gel-forming mucin genes and the genomic arrangement of these genes is well conserved throughout vertebrates. An exception is the frog Xenopus tropicalis with an expanded repertoire of at least 26 mucins of this type. Furthermore, we found that the ovomucin protein, originally identified in chicken, is characteristic of reptiles, birds, and amphibians. Muc6 is absent in teleost fish, but we now show that it is present in animals such as ghost sharks, demonstrating an early origin in vertebrate evolution. Public RNA-Seq data were analyzed with respect to mucins in zebrafish, frog, and chicken, thus allowing comparison in regard of tissue and developmental specificity. Analyses of invertebrate proteins reveal that gel-forming-mucin type of proteins is widely distributed also in this group. Their presence in Cnidaria, Porifera, and in Ctenophora (comb jellies) shows that these proteins were present early in metazoan evolution. Finally, we examined the evolution of the FCGBP protein, abundant in mucus and related to gel-forming mucins in terms of structure and localization. We demonstrate that FCGBP, ubiquitous in vertebrates, has a conserved N-terminal domain. Interestingly, this domain is also present as an N-terminal sequence in a number of bacterial proteins.
[Mh] Termos MeSH primário: Moléculas de Adesão Celular/genética
Mucinas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Moléculas de Adesão Celular/química
Moléculas de Adesão Celular/metabolismo
Células Epiteliais/metabolismo
Evolução Molecular
Genoma/genética
Seres Humanos
Cadeias de Markov
Mucina-6/química
Mucina-6/genética
Mucina-6/metabolismo
Mucinas/química
Mucinas/metabolismo
Muco
Ovomucina/química
Ovomucina/genética
Ovomucina/metabolismo
Filogenia
Análise de Sequência de RNA
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Adhesion Molecules); 0 (FCGBP protein, human); 0 (Mucin-6); 0 (Mucins); 37281-36-0 (Ovomucin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160519
[St] Status:MEDLINE
[do] DOI:10.1093/molbev/msw066


  8 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26329936
[Au] Autor:Yoshida S; Yamamoto H; Tetsui T; Kobayakawa Y; Hatano R; Mukaisho K; Hattori T; Sugihara H; Asano S
[Ad] Endereço:Department of Molecular Physiology, College of Pharmaceutical Sciences, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan.
[Ti] Título:Effects of ezrin knockdown on the structure of gastric glandular epithelia.
[So] Source:J Physiol Sci;66(1):53-65, 2016 Jan.
[Is] ISSN:1880-6562
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Ezrin, an adaptor protein that cross-links plasma membrane-associated proteins with the actin cytoskeleton, is concentrated on apical surfaces of epithelial cells, especially in microvilli of the small intestine and stomach. In the stomach, ezrin is predominantly expressed on the apical canalicular membrane of parietal cells. Transgenic ezrin knockdown mice in which the expression level of ezrin was reduced to <7% compared with the wild-type suffered from achlorhydria because of impairment of membrane fusion between tubulovesicles and apical membranes. We observed, for the first time, hypergastrinemia and foveolar hyperplasia in the gastric fundic region of the knockdown mice. Dilation of fundic glands was observed, the percentage of parietal and chief cells was reduced, and that of mucous-secreting cells was increased. The parietal cells of knockdown mice contained dilated tubulovesicles and abnormal mitochondria, and subsets of these cells contained abnormal vacuoles and multilamellar structures. Therefore, lack of ezrin not only causes achlorhydria and hypergastrinemia but also changes the structure of gastric glands, with severe perturbation of the secretory membranes of parietal cells.
[Mh] Termos MeSH primário: Proteínas do Citoesqueleto/metabolismo
Epitélio/fisiologia
Mucosa Gástrica/fisiologia
Regulação da Expressão Gênica/fisiologia
Células Parietais Gástricas/metabolismo
[Mh] Termos MeSH secundário: Acloridria/metabolismo
Animais
Anticorpos
Proteínas do Citoesqueleto/genética
Mucosa Gástrica/citologia
Gastrinas/sangue
Técnicas de Silenciamento de Genes
Lectinas
Camundongos
Camundongos Transgênicos
Microscopia de Fluorescência
Mucina-6/genética
Mucina-6/metabolismo
Mucinas/genética
Mucinas/metabolismo
Proteínas Musculares/genética
Proteínas Musculares/metabolismo
Células Parietais Gástricas/ultraestrutura
Peptídeos/genética
Peptídeos/metabolismo
RNA/genética
RNA/metabolismo
Fator Trefoil-2
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies); 0 (Cytoskeletal Proteins); 0 (Gastrins); 0 (Lectins); 0 (Muc6 protein, mouse); 0 (Mucin-6); 0 (Mucins); 0 (Muscle Proteins); 0 (Peptides); 0 (TFF2 protein, mouse); 0 (Trefoil Factor-2); 0 (ezrin); 63231-63-0 (RNA)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150903
[St] Status:MEDLINE
[do] DOI:10.1007/s12576-015-0393-4


  9 / 239 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26223471
[Au] Autor:Oo HZ; Sentani K; Mukai S; Hattori T; Shinmei S; Goto K; Sakamoto N; Naito Y; Anami K; Trang PT; Yanagihara K; Oue N; Yasui W
[Ad] Endereço:Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
[Ti] Título:Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer.
[So] Source:Gastric Cancer;19(2):443-52, 2016 Apr.
[Is] ISSN:1436-3305
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gastric cancer (GC) is the fifth commonest malignancy worldwide and still one of the leading causes of cancer-related death. The aim of this study was to identify a novel prognostic marker or therapeutic target for GC. METHODS: We analyzed candidate genes from our previous Escherichia coli ampicillin secretion trap (CAST) libraries in detail, and focused on the FKTN gene because it was overexpressed in both GC cell line CAST libraries, MKN-1 and MKN-45. RESULTS: Quantitative reverse transcriptase PCR analysis of FKTN revealed that FKTN messenger RNA was overexpressed in nine of 28 (32.1 %) GC tissue samples compared with nonneoplastic gastric mucosa. Immunostaining of fukutin showed that 297 of 695 cases (42.7 %) were positive for fukutin. Fukutin-positive GC cases were significantly associated with differentiated histological features, and advanced T grade and N grade. In addition, fukutin expression was observed more frequently in the intestinal phenotype (51 %) of GC than in other phenotypes (37 %) when defined by the expression patterns of mucin 5AC, mucin 6, mucin 2, and CD10. FKTN small interfering RNA treatment decreased GC cell proliferation. CONCLUSIONS: These results indicate that the expression of fukutin may be a key regulator for progression of GC with the intestinal mucin phenotype.
[Mh] Termos MeSH primário: Regulação Neoplásica da Expressão Gênica
Proteínas de Membrana/genética
Neoplasias Gástricas/genética
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Idoso
Ampicilina/farmacologia
Fator de Transcrição CDX2/metabolismo
Linhagem Celular Tumoral
Proliferação Celular
Escherichia coli/genética
Feminino
Biblioteca Gênica
Seres Humanos
Imunoquímica
Masculino
Proteínas de Membrana/metabolismo
Meia-Idade
Mucina-5AC/metabolismo
Mucina-2/metabolismo
Mucina-6/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CDX2 Transcription Factor); 0 (CDX2 protein, human); 0 (FKTN protein, human); 0 (MUC2 protein, human); 0 (Membrane Proteins); 0 (Mucin 5AC); 0 (Mucin-2); 0 (Mucin-6); 7C782967RD (Ampicillin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150731
[St] Status:MEDLINE
[do] DOI:10.1007/s10120-015-0511-2


  10 / 239 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25893262
[Au] Autor:Murakami T; Yao T; Mitomi H; Morimoto T; Ueyama H; Matsumoto K; Saito T; Osada T; Nagahara A; Watanabe S
[Ad] Endereço:Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. t-murakm@juntendo.ac.jp.
[Ti] Título:Clinicopathologic and immunohistochemical characteristics of gastric adenocarcinoma with enteroblastic differentiation: a study of 29 cases.
[So] Source:Gastric Cancer;19(2):498-507, 2016 Apr.
[Is] ISSN:1436-3305
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gastric adenocarcinoma with enteroblastic differentiation (GAED) has been recognized as a variant of alpha-fetoprotein (AFP)-producing gastric carcinoma, although its clinicopathologic and immunohistochemical features have not been fully elucidated. METHODS: To elucidate the clinicopathologic and immunohistochemical features of GAED, we analyzed 29 cases of GAED, including ten early and 19 advanced lesions, and compared these cases with 100 cases of conventional gastric adenocarcinoma (CGA). Immunohistochemistry for AFP, glypican 3, SALL4, and p53 was performed, and the phenotypic expression of the tumors was evaluated by immunostaining with antibodies against MUC5AC, MUC6, MUC2, CD10, and caudal-type homeobox 2 (CDX2). RESULTS: Lymphatic and venous invasion was more frequent in GAED (76 and 72 %) than in CGA (41 and 31 %; P ≤ 0.001). Lymph node metastasis was more frequently observed in GAED (69 %) than in CGA (38 %; P = 0.005), as were synchronous or metachronous liver metastases (GAED, 31 %; CGA, 6 %; P ≤ 0.001). Immunohistochemically, all GAED were positive for at least one of three enteroblastic linage markers (AFP, glypican 3, and SALL4). Glypican 3 was the most sensitive marker (83 %) for GAED, followed by SALL4 (72 %) and AFP (45 %), whereas no CGA was positive. Furthermore, the rate of positive p53 staining was 59 % in GAED. Regarding the mucin phenotype, CD10 and CDX2 were diffusely or focally expressed in all GAED cases. Invasive areas with hepatoid or enteroblastic differentiation were negative for CD10 and CDX2. CONCLUSIONS: Clinicopathologic features of GAED differ from those of CGA. GAED shows aggressive biological behavior, and is characteristically immunoreactive to AFP, glypican 3, or SALL4.
[Mh] Termos MeSH primário: Adenocarcinoma/mortalidade
Adenocarcinoma/patologia
Biomarcadores Tumorais/metabolismo
Neoplasias Gástricas/mortalidade
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Adenocarcinoma/imunologia
Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais/imunologia
Fator de Transcrição CDX2/imunologia
Fator de Transcrição CDX2/metabolismo
Feminino
Seguimentos
Glipicanas/imunologia
Glipicanas/metabolismo
Seres Humanos
Imuno-Histoquímica
Neoplasias Hepáticas/patologia
Neoplasias Hepáticas/secundário
Linfonodos/patologia
Metástase Linfática/patologia
Masculino
Meia-Idade
Mucina-5AC/imunologia
Mucina-5AC/metabolismo
Mucina-6/imunologia
Mucina-6/metabolismo
Neoplasias Gástricas/imunologia
Fatores de Transcrição/imunologia
Fatores de Transcrição/metabolismo
Proteína Supressora de Tumor p53/imunologia
Proteína Supressora de Tumor p53/metabolismo
alfa-Fetoproteínas/imunologia
alfa-Fetoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AFP protein, human); 0 (Biomarkers, Tumor); 0 (CDX2 Transcription Factor); 0 (CDX2 protein, human); 0 (GPC3 protein, human); 0 (Glypicans); 0 (MUC5AC protein, human); 0 (MUC6 protein, human); 0 (Mucin 5AC); 0 (Mucin-6); 0 (SALL4 protein, human); 0 (TP53 protein, human); 0 (Transcription Factors); 0 (Tumor Suppressor Protein p53); 0 (alpha-Fetoproteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE
[do] DOI:10.1007/s10120-015-0497-9



página 1 de 24 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde