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  1 / 1019 MEDLINE  
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[PMID]:28738073
[Au] Autor:Tashiro M; Iwata A; Yamauchi M; Shimizu K; Okada A; Ishiguro N; Inoshima Y
[Ad] Endereço:Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
[Ti] Título:The N-terminal region of serum amyloid A3 protein activates NF-κB and up-regulates MUC2 mucin mRNA expression in mouse colonic epithelial cells.
[So] Source:PLoS One;12(7):e0181796, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Serum amyloid A (SAA) is the major acute-phase protein and a precursor of amyloid A (AA) in AA amyloidosis in humans and animals. SAA isoforms have been identified in a wide variety of animals, such as SAA1, SAA2, SAA3, and SAA4 in mouse. Although the biological functions of SAA isoforms are not completely understood, recent studies have suggested that SAA3 plays a role in host defense. Expression of SAA3 is increased on the mouse colon surface in the presence of microbiota in vivo, and it increases mRNA expression of mucin 2 (MUC2) in murine colonic epithelial cells in vitro, which constitutes a protective mucus barrier in the intestinal tract. In this study, to identify responsible regions in SAA3 for MUC2 expression, recombinant murine SAA1 (rSAA1), rSAA3, and rSAA1/3, a chimera protein constructed with mature SAA1 (amino acids 1-36) and SAA3 (amino acids 37-103), and vice versa for rSAA3/1, were added to murine colonic epithelial CMT-93 cells, and the mRNA expressions of MUC2 and cytokines were measured. Inhibition assays with NF-κB inhibitor or TLR4/MD2 inhibitor were also performed. Up-regulation of MUC2 mRNA expression was strongly stimulated by rSAA3 and rSAA3/1, but not by rSAA1 or rSAA1/3. Moreover, NF-κB and TLR4/MD2 inhibitors suppressed the increase of MUC2 mRNA expression. These results suggest that the major responsible region for MUC2 expression exists in amino acids 1-36 of SAA3, and that up-regulations of MUC2 expression by SAA3 and SAA3/1 are involved with activation of NF-κB via the TLR4/MD2 complex.
[Mh] Termos MeSH primário: Colo/metabolismo
Células Epiteliais/metabolismo
Mucina-2/genética
NF-kappa B/genética
RNA Mensageiro/genética
Proteína Amiloide A Sérica/genética
Regulação para Cima/genética
[Mh] Termos MeSH secundário: Proteínas Amiloidogênicas/genética
Proteínas Amiloidogênicas/metabolismo
Amiloidose/genética
Amiloidose/metabolismo
Animais
Sequência de Bases
Linhagem Celular
Citocinas/genética
Citocinas/metabolismo
Camundongos
Mucina-2/metabolismo
NF-kappa B/metabolismo
Alinhamento de Sequência
Proteína Amiloide A Sérica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amyloidogenic Proteins); 0 (Cytokines); 0 (Muc2 protein, mouse); 0 (Mucin-2); 0 (NF-kappa B); 0 (RNA, Messenger); 0 (Serum Amyloid A Protein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181796


  2 / 1019 MEDLINE  
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[PMID]:28709869
[Au] Autor:Sharmila GR; Venkateswaran G
[Ad] Endereço:Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysuru 570020, India; Microbiology and Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysuru 570020, India.
[Ti] Título:Protective effect of bacillopeptidase CFR5 from Bacillus subtilis CFR5 on cerulein-induced pancreatitis.
[So] Source:Biochem Biophys Res Commun;491(2):455-462, 2017 Sep 16.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacillopeptidase is a serine peptidase, known for its fibrinolytic activity. However, a very little information is known about its in vivo inflammatory and/or anti-inflammatory properties. Thus, to understand whether bacillopeptidase incorporation can regulate pancreatitis or not, the cerulein-induced pancreatitis model was used, and the role of bacillopeptidase on pancreatitis was studied. In this study, 46 kDa protein was purified from Bacillus subtilis and identified as bacillopeptidase CFR5 (BPC) through MS/MS analysis. The nutritional prophylactic group was orally fed with two doses of BPC (100 µg/Kg/BW of rat) 6 h before cerulein administration and analyzed for its effect on intestine and pancreas inflammation, cytokines, and pancreatitis marker gene expression. BPC administration significantly reduced the severity of pancreatitis by decreasing serum amylase, lipase, pancreatic edema and myeloperoxidase activity. The pretreatment with BPC suppressed the pancreatic pro-inflammatory and inflammatory cytokines production including IL-6, IL-1ß, TNF-α, IL-2, IL-4, IL-5, IL-10, and IL-13 in both pancreas and serum samples. Moreover, BPC supplementation restored pancreatitis mediated disruption of intestinal barrier integrity by upregulating tight junction proteins (ZO-1, occludin), antimicrobial peptides (DEFB1, CRAMP), MUC-2, TFF3 expression and by enhancing SCFA's production. Pretreatment with BPC suppressed the intestinal inflammation with reduced cytokines production in the colon and ileal region of cerulein-induced pancreatitis. Thus, BPC based pretreatment protocol is a novel intervention to prevent acute pancreatitis.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/farmacologia
Bacillus subtilis/química
Proteínas de Bactérias/farmacologia
Edema/tratamento farmacológico
Pancreatite/tratamento farmacológico
Serina Endopeptidases/farmacologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anti-Inflamatórios não Esteroides/isolamento & purificação
Proteínas de Bactérias/isolamento & purificação
Catelicidinas/genética
Catelicidinas/metabolismo
Ceruletídeo
Citocinas/biossíntese
Defensinas/genética
Defensinas/metabolismo
Edema/induzido quimicamente
Edema/genética
Edema/patologia
Regulação da Expressão Gênica
Masculino
Mucina-2/genética
Mucina-2/metabolismo
Ocludina/genética
Ocludina/metabolismo
Pâncreas/efeitos dos fármacos
Pâncreas/metabolismo
Pâncreas/patologia
Pancreatite/induzido quimicamente
Pancreatite/genética
Pancreatite/patologia
Ratos
Ratos Wistar
Serina Endopeptidases/isolamento & purificação
Fator Trefoil-3/genética
Fator Trefoil-3/metabolismo
Proteína da Zônula de Oclusão-1/genética
Proteína da Zônula de Oclusão-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Bacterial Proteins); 0 (Cathelicidins); 0 (Cytokines); 0 (Defb1 protein, rat); 0 (Defensins); 0 (Muc2 protein, rat); 0 (Mucin-2); 0 (Occludin); 0 (Ocln protein, rat); 0 (TFF3 protein, rat); 0 (Tjp1 protein, rat); 0 (Trefoil Factor-3); 0 (Zonula Occludens-1 Protein); 0 (cathelicidin antimicrobial peptide); 888Y08971B (Ceruletide); EC 3.4.21.- (Serine Endopeptidases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170716
[St] Status:MEDLINE


  3 / 1019 MEDLINE  
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[PMID]:28700666
[Au] Autor:Wang W; Li Z; Lv Z; Zhang B; Lv H; Guo Y
[Ad] Endereço:State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, P. R. China.
[Ti] Título:Effects of Kluyveromyces marxianus supplementation on immune responses, intestinal structure and microbiota in broiler chickens.
[So] Source:PLoS One;12(7):e0180884, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To investigate the effects of Kluyveromyces marxianus on immune responses, intestinal structure and microbiota in broilers, 840 1-d-old broiler chicks were randomly divided into seven groups (eight replicates) and were fed basal diets without or with 0.25, 0.50, 1.0, 1.5, 2.0, and 2.5 g/kg of K. marxianus (2.0×1010 CFU/g). Serum and intestine samples were collected at 21 d of age. The results showed that increasing K. marxianus addition linearly reduced feed conversion ratio but linearly elevated relative thymus weight, as well as quadratically increased serum lysozyme and IgG levels, with the medium dose (1.0 g/kg) being the most effective. The ratio of villus height to crypt depth of jejunum and ileum, ileal villus height and sucrase activity, as well as the mRNA expression of ileal mucin-2, claudin-1 and sodium glucose cotransporter 1 linearly responded to the increasing K. marxianus addition. Supplemental K. marxianus at low (0.5 g/kg), medium (1.5 g/kg) and high (2.5 g/kg) dose all decreased the abundance of phylum Cyanobacteria, increased the abundance of phylum Firmicutes and genus Lactobacillus in ileum. The high dose of K. marxianus addition also reduced the abundance of order Rickettsiales and Pseudomonadales along with species Acinetobacter junii. Ileal bacterial communities between K. marxianus-treated and untreated groups formed distinctly different clusters. In summary, K. marxianus supplementation benefits feed efficiency and immune function, as well as intestinal structure in broilers, which might be attributed to the improved ileal microbial structure. Supplemental K. marxianus at high dose (2.5 g/kg) was more effective for feed efficiency and intestinal health of broilers, while the innate immunity was optimized at a medium dose (1.0 g/kg).
[Mh] Termos MeSH primário: Intestinos/microbiologia
Kluyveromyces/fisiologia
Microbiota/fisiologia
[Mh] Termos MeSH secundário: Animais
Galinhas
Claudina-1/genética
Mucina-2/genética
RNA Mensageiro/genética
Transportador 1 de Glucose-Sódio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Claudin-1); 0 (Mucin-2); 0 (RNA, Messenger); 0 (Sodium-Glucose Transporter 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180884


  4 / 1019 MEDLINE  
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[PMID]:28640835
[Au] Autor:Kuracha MR; Thomas P; Loggie BW; Govindarajan V
[Ad] Endereço:Department of Surgery, Creighton University, Omaha, Nebraska, United States of America.
[Ti] Título:Bilateral blockade of MEK- and PI3K-mediated pathways downstream of mutant KRAS as a treatment approach for peritoneal mucinous malignancies.
[So] Source:PLoS One;12(6):e0179510, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mucinous colorectal adenocarcinomas (MCAs) are clinically and morphologically distinct from nonmucinous colorectal cancers (CRCs), show a distinct spectrum of genetic alterations (higher KRAS mutations, lower p53, high MUC2), exhibit more aggressive behavior (more prone to peritoneal dissemination and lymph node involvement) and are associated with poorer response to chemotherapy with limited treatment options. Here, we report the effectiveness of combinatorial targeting of two KRAS-mediated parallel pathways in reducing MUC2 production and mucinous tumor growth in vitro and in vivo. By knockdown of mutant KRAS we show that, mutant KRAS (a) is necessary for MUC2 production in vitro and (b) synergistically engages PI3K/AKT and MEK/ERK pathways to maintain MUC2 expression in MCA cells. These results define a novel and a previously undescribed role for oncogenic KRAS in mucinous cancers. MCA cells were sensitive to MEK inhibition suggesting cellular dependence ('addiction') of KRAS-mutant MCA cells on hyperactivation of the MEK-driven pathway. Interestingly, MCA cells, though initially sensitive, were later resistant to PI3K single agent inhibition. Our studies suggest that this resistance involves dynamic rewiring of signaling circuits mediated through relief of RTK inhibition and MEK-ERK rebound activation. This resistance however, could be overcome by co-targeting of PI3K and MEK. Our studies thus provide a rational basis for MEK- and PI3K-targeted combination therapy for not only KRAS mutant MCA but also for other related mucinous neoplasms that overproduce MUC2 and have a high rate of KRAS mutations such as pseudomyxoma peritonei.
[Mh] Termos MeSH primário: Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo
Mutação
Neoplasias Peritoneais/tratamento farmacológico
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas p21(ras)/genética
Pseudomixoma Peritoneal/tratamento farmacológico
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Neoplasias Colorretais/tratamento farmacológico
Neoplasias Colorretais/genética
Neoplasias Colorretais/metabolismo
Neoplasias Colorretais/patologia
Ativação Enzimática/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Terapia de Alvo Molecular
Mucina-2/genética
Mucinas/metabolismo
Neoplasias Peritoneais/genética
Neoplasias Peritoneais/metabolismo
Neoplasias Peritoneais/patologia
Fosforilação/efeitos dos fármacos
Pseudomixoma Peritoneal/genética
Pseudomixoma Peritoneal/metabolismo
Pseudomixoma Peritoneal/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (KRAS protein, human); 0 (MUC2 protein, human); 0 (Mucin-2); 0 (Mucins); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases); EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179510


  5 / 1019 MEDLINE  
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[PMID]:28528509
[Au] Autor:Cserni G; Floris G; Koufopoulos N; Kovács A; Nonni A; Regitnig P; Stahls A; Varga Z
[Ad] Endereço:Department of Pathology, Bács-Kiskun County Teaching Hospital, Nyíri út 38, Kecskemét, 6000, Hungary. cserni@freemail.hu.
[Ti] Título:Invasive lobular carcinoma with extracellular mucin production-a novel pattern of lobular carcinomas of the breast. Clinico-pathological description of eight cases.
[So] Source:Virchows Arch;471(1):3-12, 2017 Jul.
[Is] ISSN:1432-2307
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Invasive lobular carcinoma of the breast is known to produce intracellular mucin and has been recognized in single-case reports to show extracellular mucin production, as well. This latter morphology is not only rare but must also be under- or misdiagnosed. The aim was to better characterize this entity. Cases of lobular cancers demonstrating extracellular mucin formation were identified in a multi-institutional effort and their clinical and morphologic features were assessed. Immunohistochemistry was used to characterize the E-cadherin-membrane complex, neuroendocrine differentiation, and to some extent, mucin formation. All but one of the eight cases occurred in postmenopausal patients. Extracellular mucin production was present in 5 to 50% of the tumour samples and rarely also appeared in nodal and distant metastases. The tumours were completely E-cadherin negative and showed cytoplasmic p120 positivity. The majority (n = 6/8) was also completely negative for ß-catenin, but two tumours displayed focal ß-catenin positivity in the mucinous area. MUC1 and MUC2 expression was observed in all and 7/8 tumours, respectively; neuroendocrine differentiation was present in only one. Invasive lobular carcinoma with extracellular mucin formation is a rare morphologic variant of lobular carcinoma prone to be misdiagnosed and warranting further studies.
[Mh] Termos MeSH primário: Neoplasias da Mama/patologia
Carcinoma Lobular/patologia
Mucina-1/biossíntese
Mucina-2/biossíntese
[Mh] Termos MeSH secundário: Adulto
Idoso
Biomarcadores Tumorais/análise
Neoplasias da Mama/metabolismo
Carcinoma Lobular/metabolismo
Feminino
Seres Humanos
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (MUC2 protein, human); 0 (Mucin-1); 0 (Mucin-2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170522
[St] Status:MEDLINE
[do] DOI:10.1007/s00428-017-2147-6


  6 / 1019 MEDLINE  
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[PMID]:28513991
[Au] Autor:Abdel-Latif M; El-Shahawi G; Aboelhadid SM; Abdel-Tawab H
[Ad] Endereço:Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
[Ti] Título:Immunoprotective Effect of Chitosan Particles on Hymenolepis nana - Infected Mice.
[So] Source:Scand J Immunol;86(2):83-90, 2017 Aug.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hymenolepis nana is the most commonly known intestinal cestode infecting mainly human. This study aimed to investigate the potential effect of chitosan particles (CSP) to enhance the immune system against H. nana infection. Determination of worm burden, egg output, histopathological changes, oxidative stress markers (lipid peroxidation and reduced glutathione), goblet (GCs) and mucosal mast cells (MMCs) counts in intestinal ileum was performed. In addition, levels of intestinal mRNA expression of interleukin (IL)-4, IL-9, stem cell factor (SCF), type I and II interferons (IFN)-α/ γ, tumour necrosis factor (TNF)-α, mucin 2 (MUC2) and inducible nitric oxide synthase (iNOs) were investigated using real-time PCR. The results indicated induced reductions in adult worm and egg counts in infected mice after CSP treatment. This was associated with improvement in tissue morphometric measurements and oxidative stress which were altered after infection. Expression levels of iNOs, IFN-α, IFN-γ, TNF-α and IL-9 were decreased by CSP. Conversely, expression levels of MUC2, IL-4 and SCF increased compared to infected untreated group. In addition, GCs and MMCs counts were normalized by CSP. In conclusion, this study could indicate the immunoprotective effect of CSP against H. nana infection. This was characterized with Th2 anti-inflammatory responses.
[Mh] Termos MeSH primário: Quitosana/farmacologia
Himenolepíase/prevenção & controle
Hymenolepis nana/efeitos dos fármacos
Intestinos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Quitosana/química
Quitosana/imunologia
Citocinas/genética
Citocinas/imunologia
Citocinas/metabolismo
Expressão Gênica/efeitos dos fármacos
Expressão Gênica/imunologia
Proteínas de Helminto/genética
Proteínas de Helminto/imunologia
Proteínas de Helminto/metabolismo
Interações Hospedeiro-Parasita/efeitos dos fármacos
Interações Hospedeiro-Parasita/imunologia
Seres Humanos
Himenolepíase/imunologia
Himenolepíase/parasitologia
Hymenolepis nana/imunologia
Hymenolepis nana/fisiologia
Interferon-alfa/genética
Interferon-alfa/imunologia
Interferon-alfa/metabolismo
Interferon gama/genética
Interferon gama/imunologia
Interferon gama/metabolismo
Interleucina-4/genética
Interleucina-4/imunologia
Interleucina-4/metabolismo
Intestinos/imunologia
Intestinos/parasitologia
Camundongos
Mucina-2/genética
Mucina-2/imunologia
Mucina-2/metabolismo
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/imunologia
Óxido Nítrico Sintase Tipo II/metabolismo
Contagem de Ovos de Parasitas
Tamanho da Partícula
Substâncias Protetoras/farmacologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Helminth Proteins); 0 (Interferon-alpha); 0 (Mucin-2); 0 (Protective Agents); 0 (Tumor Necrosis Factor-alpha); 207137-56-2 (Interleukin-4); 82115-62-6 (Interferon-gamma); 9012-76-4 (Chitosan); EC 1.14.13.39 (Nitric Oxide Synthase Type II)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12568


  7 / 1019 MEDLINE  
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[PMID]:28323899
[Au] Autor:Huan YW; Bengtsson RJ; MacIntyre N; Guthrie J; Finlayson H; Smith SH; Archibald AL; Ait-Ali T
[Ad] Endereço:The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian, United Kingdom.
[Ti] Título:Lawsonia intracellularis exploits ß-catenin/Wnt and Notch signalling pathways during infection of intestinal crypt to alter cell homeostasis and promote cell proliferation.
[So] Source:PLoS One;12(3):e0173782, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lawsonia intracellularis is an obligate intracellular bacterial pathogen that causes proliferative enteropathy (PE) in pigs. L. intracellularis infection causes extensive intestinal crypt cell proliferation and inhibits secretory and absorptive cell differentiation. However, the affected host upstream cellular pathways leading to PE are still unknown. ß-catenin/Wnt signalling is essential in maintaining intestinal stem cell (ISC) proliferation and self-renewal capacity, while Notch signalling governs differentiation of secretory and absorptive lineage specification. Therefore, in this report we used immunofluorescence (IF) and quantitative reverse transcriptase PCR (RTqPCR) to examine ß-catenin/Wnt and Notch-1 signalling levels in uninfected and L. intracellularis infected pig ileums at 3, 7, 14, 21 and 28 days post challenge (dpc). We found that while the significant increase in Ki67+ nuclei in crypts at the peak of L. intracellularis infection suggested enhanced cell proliferation, the expression of c-MYC and ASCL2, promoters of cell growth and ISC proliferation respectively, was down-regulated. Peak infection also coincided with enhanced cytosolic and membrane-associated ß-catenin staining and induction of AXIN2 and SOX9 transcripts, both encoding negative regulators of ß-catenin/Wnt signalling and suggesting a potential alteration to ß-catenin/Wnt signalling levels, with differential regulation of the expression of its target genes. We found that induction of HES1 and OLFM4 and the down-regulation of ATOH1 transcript levels was consistent with the increased Notch-1 signalling in crypts at the peak of infection. Interestingly, the significant down-regulation of ATOH1 transcript levels coincided with the depletion of MUC2 expression at 14 dpc, consistent with the role of ATOH1 in promoting goblet cell maturation. The lack of significant change to LGR5 transcript levels at the peak of infection suggested that the crypt hyperplasia was not due to the expansion of ISC population. Overall, simultaneous induction of Notch-1 signalling and the attenuation of ß-catenin/Wnt pathway appear to be associated with the inhibition of goblet cell maturation and enhanced crypt cell proliferation at the peak of L. intracellularis infection. Moreover, the apparent differential regulation of apoptosis between crypt and lumen cells together with the strong induction of Notch-1 signalling and the enhanced SOX9 expression along crypts 14 dpc suggest an expansion of actively dividing transit amplifying and/or absorptive progenitor cells and provide a potential basis for understanding the development and maintenance of PE.
[Mh] Termos MeSH primário: Infecções por Desulfovibrionaceae/metabolismo
Lawsonia (Bactéria)
Receptores Notch/metabolismo
Via de Sinalização Wnt/fisiologia
beta Catenina/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Caspase 3/metabolismo
Proliferação Celular/fisiologia
Infecções por Desulfovibrionaceae/patologia
Progressão da Doença
Feminino
Homeostase/fisiologia
Íleo/metabolismo
Íleo/microbiologia
Íleo/patologia
Masculino
Mucina-2/metabolismo
Distribuição Aleatória
Fatores de Transcrição SOX9/metabolismo
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mucin-2); 0 (Receptors, Notch); 0 (SOX9 Transcription Factor); 0 (beta Catenin); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173782


  8 / 1019 MEDLINE  
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[PMID]:28195642
[Au] Autor:Bosmans JW; Jongen AC; Birchenough GM; Nyström EE; Gijbels MJ; Derikx JP; Bouvy ND; Hansson GC
[Ad] Endereço:Department of Surgery, NUTRIM School for Nutrition and Translational Research in Metabolism, Maastricht, The Netherlands.
[Ti] Título:Functional mucous layer and healing of proximal colonic anastomoses in an experimental model.
[So] Source:Br J Surg;104(5):619-630, 2017 Apr.
[Is] ISSN:1365-2168
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Anastomotic leakage (AL) is the most dreaded complication after colorectal surgery, causing high morbidity and mortality. Mucus is a first line of defence against external factors in the gastrointestinal tract. In this study, the structural mucus protein Muc2 was depleted in genetically engineered mice and the effect on healing of colonic anastomoses studied in an experimental model. METHODS: Mice of different Muc2 genotypes were used in a proximal colonic AL model. Tissues were scored histologically for inflammation, bacterial translocation was determined by quantitative PCR of bacterial 16S ribosomal DNA, and epithelial cell damage was determined by assessing serum levels of intestinal fatty acid-binding protein. RESULTS: Of 22 Muc2-deficient (Muc2 ) mice, 20 developed AL, compared with seven of 22 control animals (P < 0·001). Control mice showed normal healing, whereas Muc2 mice had more inflammation with less collagen deposition and neoangiogenesis. A tendency towards higher bacterial translocation was seen in mesenteric lymph nodes and spleen in Muc2 mice. Intestinal fatty acid-binding protein levels were significantly higher in Muc2 mice compared with controls (P = 0·011). CONCLUSION: A functional mucous layer facilitates the healing of colonic anastomoses. Clinical relevance Colorectal anastomotic leakage remains the most dreaded complication after colorectal surgery. It is known that the aetiology of anastomotic leakage is multifactorial, and a role is suggested for the interaction between intraluminal content and mucosa. In this murine model of proximal colonic anastomotic leakage, the authors investigated the mucous layer at the intestinal mucosa, as the first line of defence, and found that a normal, functioning mucous layer is essential in the healing process of colonic anastomoses. Further research on anastomotic healing should focus on positively influencing the mucous layer to promote better postoperative recovery.
[Mh] Termos MeSH primário: Anastomose Cirúrgica
Cirurgia Colorretal
Cicatrização/fisiologia
[Mh] Termos MeSH secundário: Fístula Anastomótica/prevenção & controle
Animais
Translocação Bacteriana
Colo/cirurgia
Dinoprostona/farmacologia
Ensaio de Imunoadsorção Enzimática
Proteínas de Ligação a Ácido Graxo/sangue
Genótipo
Mucosa Intestinal
Camundongos
Modelos Teóricos
Mucina-2/genética
Reação em Cadeia da Polimerase em Tempo Real
Cicatrização/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acid-Binding Proteins); 0 (Muc2 protein, mouse); 0 (Mucin-2); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1002/bjs.10456


  9 / 1019 MEDLINE  
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[PMID]:28138305
[Au] Autor:Pillai K; Akhter J; Mekkawy A; Chua TC; Morris DL
[Ad] Endereço:Department of Surgery, University of New South Wales, St. George Hospital, Kogarah, Sydney, NSW, AUSTRALIA.
[Ti] Título:Physical and chemical characteristics of mucin secreted by pseudomyxoma peritonei (PMP).
[So] Source:Int J Med Sci;14(1):18-28, 2017.
[Is] ISSN:1449-1907
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Pseudomyxoma peritonei (PMP) is a rare disease with excess intraperitoneal mucin secretion. Treatment involves laparotomy, cytoreduction and chemotherapy that is very invasive with patients often acquiring numerous compromises. Hence a mucolytic comprising of bromelain and N-acetyl cystein has been developed to solubilise mucin in situ for removal by catherization. Owing to differences in mucin appearance and hardness, dissolution varies. Therefore the current study investigates the inter-mucin physical and chemical characteristics, in order to reformulate an effective mucolytic for all mucin. PMP mucin, from the three categories (soft, semi hard and hard mucin) was solubilised and then various physical characteristics such as turbidity, density, kinematic viscosity were measured. The water content and the density of solid mucin were also determined. This was followed by the determination of sialic acid, glucose, lipid, Thiol (S-S and S-H) content of the samples. Lastly, the distribution of MUC2, MUC5B and MUC5AC was determined using western blot technique. Both turbidity and kinematic viscosity and sialic acid content increased linearly as the hardness of mucin increased. However, density, hydration, protein, glucose, lipid and sulfhydryl and disulphide content decreased linearly as hardness of mucin increased. The distribution ratio of mucins (MUC2:MUC5B:MUC5AC) in soft mucin is 2.25:1.5:1.0, semi hard mucin is 1:1:1 and hard mucin is 3:2:1. The difference in texture and hardness of mucin may be due to cellular content, hydration, glucose, protein, lipids, thiol and MUC distribution. Soft mucin is solely made of glycoprotein whilst the others contained cellular materials.
[Mh] Termos MeSH primário: Mucinas/química
Mucinas/secreção
Muco/química
Neoplasias Peritoneais/metabolismo
Pseudomixoma Peritoneal/metabolismo
[Mh] Termos MeSH secundário: Glucose/análise
Seres Humanos
Lipídeos/análise
Mucina-5AC/análise
Mucina-2/análise
Mucina-5B/análise
Muco/secreção
Ácido N-Acetilneuramínico/análise
Compostos de Sulfidrila/análise
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids); 0 (MUC2 protein, human); 0 (MUC5AC protein, human); 0 (MUC5B protein, human); 0 (Mucin 5AC); 0 (Mucin-2); 0 (Mucin-5B); 0 (Mucins); 0 (Sulfhydryl Compounds); GZP2782OP0 (N-Acetylneuraminic Acid); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.7150/ijms.16422


  10 / 1019 MEDLINE  
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[PMID]:28106824
[Au] Autor:Woodfint RM; Chen PR; Ahn J; Suh Y; Hwang S; Lee SS; Lee K
[Ad] Endereço:Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA. woodfint.1@osu.edu.
[Ti] Título:Identification of the MUC2 Promoter as a Strong Promoter for Intestinal Gene Expression through Generation of Transgenic Quail Expressing GFP in Gut Epithelial Cells.
[So] Source:Int J Mol Sci;18(1), 2017 Jan 19.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Identification of tissue- and stage-specific gene promoters is valuable for delineating the functional roles of specific genes in genetically engineered animals. Here, through the comparison of gene expression in different tissues by analysis of a microarray database, the intestinal specificity of mucin 2 ( ) expression was identified in mice and humans, and further confirmed in chickens by RT-PCR (reverse transcription-PCR) analysis. An analysis of -acting elements in avian gene promoters revealed conservation of binding sites, within a 2.9 kb proximal promoter region, for transcription factors such as caudal type homeobox 2 (CDX2), GATA binding protein 4 (GATA4), hepatocyte nuclear factor 4 α (HNF4A), and transcription factor 4 (TCF4) that are important for maintaining intestinal homeostasis and functional integrity. By generating transgenic quail, we demonstrated that the 2.9 kb chicken promoter could drive green fluorescent protein (GFP) reporter expression exclusively in the small intestine, large intestine, and ceca. Fluorescence image analysis further revealed GFP expression in intestine epithelial cells. The GFP expression was barely detectable in the embryonic intestine, but increased during post-hatch development. The spatiotemporal expression pattern of the reporter gene confirmed that the 2.9 kb promoter could retain the regulatory element to drive expression of target genes in intestinal tissues after hatching. This new transgene expression system, using the promoter, will provide a new method of overexpressing target genes to study gene function in the avian intestine.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Regulação da Expressão Gênica
Proteínas de Fluorescência Verde/metabolismo
Intestinos/citologia
Mucina-2/genética
Regiões Promotoras Genéticas
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Sítios de Ligação
Western Blotting
Galinhas
Seres Humanos
Camundongos
Mucina-2/metabolismo
Análise de Sequência com Séries de Oligonucleotídeos
Especificidade de Órgãos/genética
Codorniz
Reprodutibilidade dos Testes
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MUC2 protein, human); 0 (Muc2 protein, mouse); 0 (Mucin-2); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE



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