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[PMID]:29236393
[Au] Autor:Lutsenko TN; Kovalenko MV; Galkin OY
[Ti] Título:Validation of biological activity testing procedure of recombinant human interleukin-7.
[So] Source:Ukr Biochem J;89(1):82-9, 2017 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Validation procedure for method of monitoring the biological activity of reсombinant human interleukin-7 has been developed and conducted according to the requirements of national and international recommendations. This method is based on the ability of recombinant human interleukin-7 to induce proliferation of T lymphocytes. It has been shown that to control the biological activity of recombinant human interleukin-7 peripheral blood mononuclear cells (PBMCs) derived from blood or cell lines can be used. Validation charac­teristics that should be determined depend on the method, type of product or object test/measurement and biological test systems used in research. The validation procedure for the method of control of biological activity of recombinant human interleukin-7 in peripheral blood mononuclear cells showed satisfactory results on all parameters tested such as specificity, accuracy, precision and linearity.
[Mh] Termos MeSH primário: Bioensaio/normas
Interleucina-7/farmacologia
Fito-Hemaglutininas/farmacologia
Linfócitos T/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Proliferação Celular/efeitos dos fármacos
Separação Celular/métodos
Seres Humanos
Leucócitos Mononucleares/citologia
Leucócitos Mononucleares/imunologia
Ativação Linfocitária/efeitos dos fármacos
Camundongos
Cultura Primária de Células
Proteínas Recombinantes/farmacologia
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Linfócitos T/citologia
Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (IL7 protein, human); 0 (Interleukin-7); 0 (Phytohemagglutinins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.082


  2 / 11026 MEDLINE  
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[PMID]:28929596
[Au] Autor:de Melo KM; de Moraes-Pinto MI; Andrade LEC; Salomão R; Brunialti MKC; Ferreira VS; Costa-Carvalho BT
[Ad] Endereço:Department of Pediatrics, Escola Paulista de Medicina, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil.
[Ti] Título:Hypogammaglobulinemia in children: a warning sign to look deeply?
[So] Source:APMIS;125(10):902-909, 2017 Oct.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:This study investigated phenotypic and functional characteristics of lymphocytes in children with common variable immunodeficiency (CVID) and unclassified hypogammaglobulinemia (UH), as well as B-cell subsets in non-consanguineous parents. Blood samples of 30 children, CVID (n = 9), UH (n = 9), healthy donors HD (n = 12), and 19 adults (parents and controls) were labeled by a combination of surface markers to identify CD4, CD8 T-cell and B-cell subpopulations. T-cell cytokine production in children was analyzed in vitro after stimulation with phytohemagglutinin (PHA) and tetanus toxoid. We observed low percentages of switched memory B cells in children with CVID, increase in total CD4 T-cell counts, and high percentages of transitional B cells only in UH group. Analysis of T-cell immunity showed that CVID children had decreased percentages of CD8 IFN-γ-producing cells after stimulation with PHA and tetanus toxoid. Parent of children with CVID had low percentages of naive B cell and increased percentages of memory B cells in comparison with controls. These results suggest that (i) early combined immune defect in children with CVID and (ii) a possible familial B-cell disturbance in pediatric CVID.
[Mh] Termos MeSH primário: Linfócitos B/imunologia
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Imunodeficiência de Variável Comum/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Criança
Pré-Escolar
Citocinas/secreção
Feminino
Seres Humanos
Fatores Imunológicos
Masculino
Meia-Idade
Fito-Hemaglutininas/imunologia
Toxoide Tetânico/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Immunologic Factors); 0 (Phytohemagglutinins); 0 (Tetanus Toxoid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12738


  3 / 11026 MEDLINE  
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[PMID]:28779561
[Au] Autor:Signorello MG; Leoncini G
[Ad] Endereço:.
[Ti] Título:The molecular mechanisms involved in lectin-induced human platelet aggregation.
[So] Source:Biol Chem;398(12):1335-1346, 2017 Nov 27.
[Is] ISSN:1437-4315
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We have compared the effect of three legume lectins, wheat germ agglutinin (WGA), Phaseolus vulgaris agglutinin (PHA) and Lens culinaris agglutinin (LCA), on the function of human platelets. We have found that WGA is more active than PHA in stimulating platelet activation/aggregation, while LCA has no effect. Studies on the mechanisms involved show that WGA and PHA induce phosphorylation/activation of PLCγ2 and increase [Ca2+]i. For the first time, it has been shown that Src/Syk pathway, the adapter protein SLP-76 and the exchange protein VAV, participate in the PLCγ2 activation by these lectins. Moreover WGA and PHA stimulate the PI3K/AKT pathway. PI3K, through its product phosphatidylinositol-3,4,5-trisphosphate activates Bruton's tyrosine kinase (BTK) and contributes to PLCγ2 activation. In conclusion, our findings suggest that PLCγ2 activation induced by WGA and PHA is regulated by Src/Syk and by PI3K/BTK pathways through their concerted action.
[Mh] Termos MeSH primário: Fito-Hemaglutininas/farmacologia
Lectinas de Plantas/farmacologia
Agregação Plaquetária/efeitos dos fármacos
Aglutininas do Germe de Trigo/farmacologia
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Seres Humanos
Fosfolipase C gama/metabolismo
Fito-Hemaglutininas/química
Lectinas de Plantas/química
Relação Estrutura-Atividade
Aglutininas do Germe de Trigo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytohemagglutinins); 0 (Plant Lectins); 0 (Wheat Germ Agglutinins); 0 (lentil lectin); EC 3.1.4.3 (Phospholipase C gamma)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170806
[St] Status:MEDLINE


  4 / 11026 MEDLINE  
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[PMID]:28594941
[Au] Autor:Yin K; Zhu R; Wang S; Zhao RC
[Ad] Endereço:Centre of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
[Ti] Título:Low level laser (LLL) attenuate LPS-induced inflammatory responses in mesenchymal stem cells via the suppression of NF-κB signaling pathway in vitro.
[So] Source:PLoS One;12(6):e0179175, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Considering promising results in animal models and patients, therapeutic use of MSCs for immune disease is likely to undergo continued evaluation. Low-lever laser (LLL) has been widely applied to retard the inflammatory reaction. LLL treatment can potentially be applied in anti-inflammatory therapy followed by stem cell therapy. AIM OF THE STUDY: The purpose of this study was to investigate the effect of LLL (660 nm) on the inflammatory reaction induced by LPS in human adipose derived mesenchymal stem cells (hADSCs) and pertinent mechanism. MATERIALS AND METHODS: Anti-inflammatory activity of LLL was investigated by LPS-induced mesenchymal stem cells. The production and expression of pro-inflammatory cytokines were evaluated by ELISA kits and RT-qPCR. Nuclear translocation of NF-κB was indicated by immunofluorescent staining. Phosphorylation status of NF-κB p65 and IκBα were illustrated by western blot assay. ROS generation was measured with CM-H2DCFDA, and NO secretion was determined by DAF-FM. We studied surface expression of lymphocyte activation markers when Purified peripheral blood mononuclear cell (PBMC) were activated by phytohaemagglutinin (PHA) in the presence of 3 types of treated MSCs. RESULTS: LLL reduced the secretion of IL-1ß, IL-6, IL8, ROS and NO in LPS treated MSCs. Immunofluorescent assay demonstrated the nuclear translocation decrease of NF-κB in LLL treated LPS induced MSCs. Western blot analysis also suggested that LLL suppressed NF-κB activation via regulating the phosphorylation of p65 and IκBα. MSC significantly reduced the expression of activation markers CD25 and CD69 on PHA-stimulated lymphocytes. CONCLUSION: The results indicate that LLL suppressed the activation of NF-κB signaling pathway in LPS treated MSCs through inhibiting phosphorylation of p65 and IκBα, which results in good anti-inflammatory effect. In addition, LLL attenuated activation-associated markers CD25 and CD69 in co-cultures of PBMC and 3 types of treated MSCs.
[Mh] Termos MeSH primário: Inflamação/metabolismo
Inflamação/patologia
Lasers
Células Mesenquimais Estromais/patologia
Células Mesenquimais Estromais/efeitos da radiação
NF-kappa B/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Núcleo Celular/efeitos dos fármacos
Núcleo Celular/metabolismo
Núcleo Celular/efeitos da radiação
Citocinas/genética
Citocinas/metabolismo
Regulação da Expressão Gênica/efeitos da radiação
Seres Humanos
Inflamação/genética
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/metabolismo
Leucócitos Mononucleares/efeitos da radiação
Lipopolissacarídeos
Células Mesenquimais Estromais/efeitos dos fármacos
Células Mesenquimais Estromais/metabolismo
Inibidor de NF-kappaB alfa/metabolismo
Óxido Nítrico/metabolismo
Fosforilação/efeitos dos fármacos
Fosforilação/efeitos da radiação
Fito-Hemaglutininas/farmacologia
Transporte Proteico/efeitos dos fármacos
Transporte Proteico/efeitos da radiação
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/efeitos da radiação
Fator de Transcrição RelA/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Phytohemagglutinins); 0 (RNA, Messenger); 0 (Reactive Oxygen Species); 0 (Transcription Factor RelA); 139874-52-5 (NF-KappaB Inhibitor alpha); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179175


  5 / 11026 MEDLINE  
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[PMID]:28392318
[Au] Autor:Bosco MJ; Hou H; Mao L; Wu X; Ramroop KD; Lu Y; Mao L; Zhou Y; Sun Z; Wang F
[Ad] Endereço:Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
[Ti] Título:The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients.
[So] Source:Int J Infect Dis;59:55-60, 2017 Jun.
[Is] ISSN:1878-3511
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The results of the T-SPOT.TB (T-SPOT) assay are reduced in immunocompromised patients with active tuberculosis (ATB), and it is difficult using T-SPOT results to distinguish ATB from latent tuberculosis infection (LTBI) in this condition. The aim of this study was to determine the performance of the TBAg/PHA ratio in T-SPOT assay in the diagnosis of ATB in immunocompromised patients. METHODS: One hundred and forty three immunocompromised ATB patients and 124 LTBI individuals were diagnosed according to conventional tests and T-SPOT assay. RESULTS: The results of T-SPOT assay are of no value in the diagnosis of ATB in immunocompromised patients. However, the number of phytohaemagglutinin (PHA) spot-forming cells (sfc) in T-SPOT assay was substantially decreased in immunocompromised ATB patients compared with that in LTBI individuals. Receiver operating characteristic (ROC) analysis revealed that a further calculation of the TBAg/PHA ratio (the larger of the ESAT-6/PHA and CFP-10/PHA) showed a better performance in distinguishing these two diseases. Using the threshold value of 0.316, the sensitivity and specificity for distinguishing immunocompromised ATB patients from LTBI individuals were respectively 79.21 and 94.05%. CONCLUSIONS: Our findings suggest that the TBAg/PHA ratio might have some significance for the diagnosis of TB disease in immunocompromised patients.
[Mh] Termos MeSH primário: Antígenos de Bactérias/análise
Tuberculose Latente/diagnóstico
Mycobacterium/imunologia
Fito-Hemaglutininas/análise
Tuberculose/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Hospedeiro Imunocomprometido
Tuberculose Latente/microbiologia
Masculino
Meia-Idade
Curva ROC
Sensibilidade e Especificidade
Tuberculose/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Phytohemagglutinins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE


  6 / 11026 MEDLINE  
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[PMID]:28370210
[Au] Autor:de Ruiter K; Tahapary DL; Wammes LJ; Wiria AE; Hamid F; van Lieshout L; Smit JWA; Houwing-Duistermaat JJ; Sartono E; Supali T; Yazdanbakhsh M
[Ad] Endereço:Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
[Ti] Título:The effect of three-monthly albendazole treatment on Th2 responses: Differential effects on IgE and IL-5.
[So] Source:Parasite Immunol;39(6), 2017 Jun.
[Is] ISSN:1365-3024
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Helminth parasites induce a strong Th2 response, characterized by high levels of IgE and elevated signature cytokines such as IL-5. As many global deworming programmes are underway, there is concern that this might lead to emergence of Th1-mediated pathologies when the counterbalancing helminth-induced Th2 response is absent. Therefore, we assessed the effect of deworming on Th2-mediated responses in a household-clustered randomized controlled trial in Indonesia. Total plasma IgE and whole-blood IL-5 responses to mitogen phytohaemagglutinin (PHA) were measured in 1494 and 682 subjects, respectively, at baseline, 9 and 21 months after three-monthly single-dose treatment with albendazole or placebo. Anthelmintic treatment did not result in complete removal of helminth infections in the community. However, treatment significantly decreased IgE levels in albendazole- compared to placebo-treated subjects. IL-5 responses to PHA were not significantly affected by anthelmintic treatment and tended to increase in albendazole-treated subjects, indicating that intensive treatment of helminth parasites has different outcomes on B-cell (IgE levels) and T-cell (IL-5) responses. The data shows that 2 years of deworming can have differential effects on responses typified as Th2-mediated, which needs to be taken into account when examining the impact of helminths on noncommunicable diseases.
[Mh] Termos MeSH primário: Albendazol/uso terapêutico
Anti-Helmínticos/uso terapêutico
Helmintíase/tratamento farmacológico
Imunoglobulina E/sangue
Interleucina-5/metabolismo
Células Th2/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Animais
Método Duplo-Cego
Feminino
Helmintíase/epidemiologia
Helmintíase/parasitologia
Helmintos/imunologia
Seres Humanos
Masculino
Fito-Hemaglutininas/imunologia
Células Th2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Interleukin-5); 0 (Phytohemagglutinins); 37341-29-0 (Immunoglobulin E); F4216019LN (Albendazole)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/pim.12428


  7 / 11026 MEDLINE  
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[PMID]:28366645
[Au] Autor:Siedlik JA; Deckert JA; Benedict SH; Bhatta A; Dunbar AJ; Vardiman JP; Gallagher PM
[Ad] Endereço:Department of Exercise Science and Pre-Health Professions, Creighton University, Omaha, NE, United States.
[Ti] Título:T cell activation and proliferation following acute exercise in human subjects is altered by storage conditions and mitogen selection.
[So] Source:J Immunol Methods;446:7-14, 2017 Jul.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Recent work investigating exercise induced changes in immunocompetence suggests that some of the ambiguity in the literature is resultant from different cell isolation protocols and mitogen selection. To understand this effect, we compared post-exercise measures of T cell activation and proliferation using two different stimulation methods (costimulation through CD28 or stimulation with phytohaemagglutinin [PHA]). Further, we investigated whether exercise induced changes are maintained when T cell isolation from whole blood is delayed overnight in either a room temperature or chilled (4°C) environment. As expected, an increased proliferation response was observed post-exercise in T cells isolated from whole blood of previously trained individuals immediately after blood collection. Also, cells stimulated with PHA after resting overnight in whole blood were not adversely impacted by the storage conditions. In contrast, allowing cells to rest overnight in whole blood prior to stimulation through CD28, lessened the proliferation observed by cells following exercise rendering both the room temperature and chilled samples closer to the results seen in the control condition. Changes in early markers of activation (CD25), followed a similar pattern, with activation in PHA stimulated cells remaining fairly robust after overnight storage; whereas cell activation following stimulation through CD3+CD28 was disproportionately decreased by the influence of overnight storage. These findings indicate that decisions regarding cell stimulation methods need to be paired with the timeline for T cell isolation from whole blood. These considerations will be especially important for field based studies of immunocompetence where there is a delay in getting whole blood samples to a lab for processing as well as clinical applications where a failure to isolate T cells in a timely manner may result in loss of the response of interest.
[Mh] Termos MeSH primário: Preservação de Sangue/métodos
Proliferação Celular
Exercício
Ativação Linfocitária
Manejo de Espécimes/métodos
Linfócitos T/fisiologia
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/sangue
Antígenos CD28/imunologia
Antígenos CD28/metabolismo
Complexo CD3/imunologia
Complexo CD3/metabolismo
Citometria de Fluxo
Voluntários Saudáveis
Seres Humanos
Masculino
Mitógenos
Fito-Hemaglutininas/farmacologia
Refrigeração
Linfócitos T/imunologia
Temperatura Ambiente
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (CD28 Antigens); 0 (CD3 Complex); 0 (Mitogens); 0 (Phytohemagglutinins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


  8 / 11026 MEDLINE  
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[PMID]:28271177
[Au] Autor:Desprat JL; Lengagne T; Mondy N
[Ad] Endereço:UMR 5023 LEHNA Écologie des Hydrosystèmes Naturels et Anthropisés, UnivLyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, F-69622, Villeurbanne, France. julia.desprat@univ-lyon1.fr.
[Ti] Título:Immune challenges and visual signalling in tree frogs.
[So] Source:Naturwissenschaften;104(3-4):21, 2017 Apr.
[Is] ISSN:1432-1904
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In animals, mate-choice is often based on sexual signals that carry information and help the receiver make the best choice to improve the receiver's fitness. Orange visual sexual signals have been hypothesised to carry immune information because they are often due to carotenoid pigments which are also involved in immunity response. Although many studies have focused on the direct relationships between coloration and immunocompetence, few studies have simultaneously studied immunocompetent response and coloration variation after an immune challenge. We tested this hypothesis on starved and ad libitum-fed males of the European tree frog Hyla arborea. Our results show that male coloration is not a reliable indicator of its immune response capacity in this species. However, after an immune challenge induced by a PHA (Phaseolus vulgaris phytohaemagglutinin) injection, starved males presented a significant coloration loss and this alteration was related to the immune response intensity. Taken together, these results suggest that the brighter (lighter) coloration may be used as a cue by female to exclude males with a recent immune challenge, due to diseases or parasites for example.
[Mh] Termos MeSH primário: Anuros/fisiologia
Pigmentação/imunologia
[Mh] Termos MeSH secundário: Comunicação Animal
Animais
Anuros/imunologia
Carotenoides/imunologia
Feminino
Imunocompetência/imunologia
Fatores Imunológicos/farmacologia
Masculino
Casamento
Phaseolus/química
Fito-Hemaglutininas/imunologia
Fito-Hemaglutininas/farmacologia
Pigmentação/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunologic Factors); 0 (Phytohemagglutinins); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1007/s00114-017-1436-x


  9 / 11026 MEDLINE  
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[PMID]:28168727
[Au] Autor:Moafi M; Rezvan H; Sherkat R; Taleban R; Asilian A; Hamid Zarkesh-Esfahani S; Nilforoushzadeh MA; Jaffary F; Mansourian M; Sokhanvari F; Ansari N
[Ad] Endereço:Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
[Ti] Título:Comparison of pro-inflammatory cytokines of non-healing and healing cutaneous leishmaniasis.
[So] Source:Scand J Immunol;85(4):291-299, 2017 Apr.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cutaneous leishmaniasis (CL) heals spontaneously within several weeks or months, but, in rare cases, CL-active lesions last for many years. In this study, we assessed cell-mediated immunity in non-healing CL through the measurement of three pro-inflammatory cytokines: Interferon-γ (IFN-γ), IL-17a and CXCL-11. For this, 32 patients afflicted with healing or non-healing CL were recruited in this study. Peripheral blood mononuclear cells (PBMCs) of every patient were treated with three antigens: purified protein derivative (PPD), soluble Leishmania antigen (SLA) and phytohaemagglutinin (PHA). Cytokine quantification was performed using enzyme-linked immunosorbent assay (ELISA) method. Results of our study showed that neither cytokine produced in the presence of a PPD stimulator (as an irrelevant antigen) significantly differed between the healing and non-healing groups (P-value ≥0.05 for all of them). However, IFN-γ, CXCL-11 and IL-17a levels produced in the presence of PHA or SLA were significantly higher within the healing than in the non-healing group (P-value <0.01 for all of them). It seems that appropriate levels of IFN-γ, as well as IL-17a and CXCL-11, contribute to the control of Leishmania infection.
[Mh] Termos MeSH primário: Quimiocina CXCL11/sangue
Interferon gama/sangue
Interleucina-17/sangue
Leishmania/imunologia
Leishmaniose Cutânea/sangue
Leishmaniose Cutânea/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antígenos de Protozoários/imunologia
Criança
Estudos Transversais
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Leishmaniose Cutânea/parasitologia
Masculino
Meia-Idade
Fito-Hemaglutininas/imunologia
Tuberculina/imunologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (CXCL11 protein, human); 0 (Chemokine CXCL11); 0 (IL17A protein, human); 0 (Interleukin-17); 0 (Phytohemagglutinins); 0 (Tuberculin); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12534


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[PMID]:28091904
[Au] Autor:Emel'yanova AG; Grechenko VV; Petrova NV; Shilovskii IP; Gorbunov EA; Tarasov SA; Khaitov MR; Morozov SG; Epshtein OI
[Ad] Endereço:1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology, Moscow, Russia. agemelyanova@gmail.com.
[Ti] Título:Effects of Release-Active Antibodies to CD4 Receptor on the Level of lck-Kinase in Cultured Mononuclear Cells from Human Peripheral Blood.
[So] Source:Bull Exp Biol Med;162(3):323-326, 2017 Jan.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For evaluation of effects of release-active antibodies to CD4 on cultured lymphocytes from human peripheral blood, we measured intracellular content of lck-kinase cell-based ELISA. In cells treated with release-active antibodies to CD4, the content of intracellular lck-kinase significantly (p<0.01) decreased in comparison with the control (purified water processed in a similar way). Phytohemagglutinin had no effect on the concentration of lck-kinase in cells. The decrease in the content of CD4-associated lck protein suggests that the preparation enhanced intracellular coupling of lck-kinase with T-cell receptor and potentiated T-cell immune response.
[Mh] Termos MeSH primário: Anticorpos/farmacologia
Antígenos CD4/genética
Linfócitos T CD4-Positivos/efeitos dos fármacos
Proteína Tirosina Quinase p56(lck) Linfócito-Específica/genética
[Mh] Termos MeSH secundário: Adulto
Antígenos CD4/imunologia
Linfócitos T CD4-Positivos/citologia
Linfócitos T CD4-Positivos/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Regulação da Expressão Gênica
Seres Humanos
Ativação Linfocitária/efeitos dos fármacos
Proteína Tirosina Quinase p56(lck) Linfócito-Específica/imunologia
Masculino
Fito-Hemaglutininas/farmacologia
Cultura Primária de Células
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (CD4 Antigens); 0 (Phytohemagglutinins); EC 2.7.10.2 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck))
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE
[do] DOI:10.1007/s10517-017-3606-4



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