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[PMID]:29203862
[Au] Autor:Alculumbre SG; Saint-André V; Di Domizio J; Vargas P; Sirven P; Bost P; Maurin M; Maiuri P; Wery M; Roman MS; Savey L; Touzot M; Terrier B; Saadoun D; Conrad C; Gilliet M; Morillon A; Soumelis V
[Ad] Endereço:Institut Curie, Centre de Recherche, PSL Research University, Paris, France.
[Ti] Título:Diversification of human plasmacytoid predendritic cells in response to a single stimulus.
[So] Source:Nat Immunol;19(1):63-75, 2018 Jan.
[Is] ISSN:1529-2916
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Innate immune cells adjust to microbial and inflammatory stimuli through a process termed environmental plasticity, which links a given individual stimulus to a unique activated state. Here, we report that activation of human plasmacytoid predendritic cells (pDCs) with a single microbial or cytokine stimulus triggers cell diversification into three stable subpopulations (P1-P3). P1-pDCs (PD-L1 CD80 ) displayed a plasmacytoid morphology and specialization for type I interferon production. P3-pDCs (PD-L1 CD80 ) adopted a dendritic morphology and adaptive immune functions. P2-pDCs (PD-L1 CD80 ) displayed both innate and adaptive functions. Each subpopulation expressed a specific coding- and long-noncoding-RNA signature and was stable after secondary stimulation. P1-pDCs were detected in samples from patients with lupus or psoriasis. pDC diversification was independent of cell divisions or preexisting heterogeneity within steady-state pDCs but was controlled by a TNF autocrine and/or paracrine communication loop. Our findings reveal a novel mechanism for diversity and division of labor in innate immune cells.
[Mh] Termos MeSH primário: Citocinas/imunologia
Células Dendríticas/imunologia
Expressão Gênica/imunologia
Imunidade Inata/imunologia
[Mh] Termos MeSH secundário: Imunidade Adaptativa/imunologia
Antígeno B7-1/imunologia
Antígeno B7-1/metabolismo
Antígeno B7-H1/imunologia
Antígeno B7-H1/metabolismo
Células Cultivadas
Citocinas/genética
Citocinas/metabolismo
Células Dendríticas/metabolismo
Células Dendríticas/ultraestrutura
Perfilação da Expressão Gênica/métodos
Seres Humanos
Interferon Tipo I/genética
Interferon Tipo I/imunologia
Interferon Tipo I/metabolismo
Lúpus Eritematoso Sistêmico/imunologia
Microscopia Eletrônica de Transmissão
Orthomyxoviridae/imunologia
Psoríase/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7-1 Antigen); 0 (B7-H1 Antigen); 0 (CD274 protein, human); 0 (Cytokines); 0 (Interferon Type I)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1038/s41590-017-0012-z


  2 / 4746 MEDLINE  
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[PMID]:28889065
[Au] Autor:Lu YB; Xiao DQ; Liang KD; Zhang JA; Wang WD; Yu SY; Zheng BY; Gao YC; Dai YC; Jia Y; Chen C; Zhuang ZG; Wang X; Fu XX; Zhou Y; Zhong J; Chen ZW; Xu JF
[Ad] Endereço:Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Department of Laboratory Medicine, Dongguan 5th Hospital, Dongguan 523000, China.
[Ti] Título:Profiling dendritic cell subsets in the patients with active pulmonary tuberculosis.
[So] Source:Mol Immunol;91:86-96, 2017 11.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dendritic cell (DC) plays an important role in the immune response against pulmonary tuberculosis. However, the phenotypic profile of DC subsets in peripheral blood in individuals with active pulmonary tuberculosis (APT) is still inconclusive. Here, we demonstrated that the absolute numbers of total DC (tDC), myeloid DC (mDC) and plasmacytoid DC (pDC) in individuals with APT were decreased compared to healthy controls (HCs). The decreased number of DCs, especially of pDC, seems to be a useful diagnostic marker of APT. Meanwhile, the number of DCs was associated with the prolonged/complicated TB, ATD treatment effect and lymphocyte immune reactions, as manifested that relapsed APT patients with a higher number of tDC and lower number of pDC compared to newly diagnosed patients. Interestingly, mDC from APT patients displayed high expressions of CD83 and CCR7, but pDC displayed low expressions of CD83 and CCR7. Moreover, DCs from APT patients expressed lower levels of HLA-DR and CD80, but expressed a higher level of CD86 than those from HCs. However, the antigen uptake capacity of DC subsets was not different between APT and HCs, despite the antigen uptake capacity of pDC was much lower than that of mDC in both APT patients and HCs. Our data represent a systematic profile of DC subsets in the blood of APT patients, and would represent a useful biomarker for APT.
[Mh] Termos MeSH primário: Células Dendríticas/imunologia
Regulação da Expressão Gênica/imunologia
Tuberculose Pulmonar/imunologia
[Mh] Termos MeSH secundário: Doença Aguda
Adolescente
Adulto
Idoso
Antígenos CD/imunologia
Antígeno B7-1/imunologia
Células Dendríticas/patologia
Feminino
Antígenos HLA-DR/imunologia
Seres Humanos
Imunoglobulinas/imunologia
Masculino
Glicoproteínas de Membrana/imunologia
Meia-Idade
Receptores CCR7/imunologia
Tuberculose Pulmonar/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (B7-1 Antigen); 0 (CCR7 protein, human); 0 (CD83 antigen); 0 (HLA-DR Antigens); 0 (Immunoglobulins); 0 (Membrane Glycoproteins); 0 (Receptors, CCR7)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170911
[St] Status:MEDLINE


  3 / 4746 MEDLINE  
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[PMID]:28850584
[Au] Autor:Turner JS; Benet ZL; Grigorova I
[Ad] Endereço:Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America.
[Ti] Título:Transiently antigen primed B cells can generate multiple subsets of memory cells.
[So] Source:PLoS One;12(8):e0183877, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Memory B cells are long-lived cells that generate a more vigorous response upon recognition of antigen (Ag) and T cell help than naïve B cells and ensure maintenance of durable humoral immunity. Functionally distinct subsets of murine memory B cells have been identified based on isotype switching of BCRs and surface expression of the co-stimulatory molecule CD80 and co-inhibitory molecule PD-L2. Memory B cells in a subpopulation with low surface expression of CD80 and PD-L2 are predominantly non-isotype switched and can be efficiently recruited into germinal centers (GCs) in secondary responses. In contrast, a CD80 and PD-L2 positive subset arises predominantly from GCs and can quickly differentiate into antibody-secreting plasma cells (PCs). Here we demonstrate that single transient acquisition of Ag by B cells may be sufficient for their long-term participation in GC responses and for development of various memory B cell subsets including CD80 and PD-L2 positive effector-like memory cells that rapidly differentiate into class-switched PCs during recall responses.
[Mh] Termos MeSH primário: Subpopulações de Linfócitos B/imunologia
Linfócitos B/imunologia
Diferenciação Celular/fisiologia
Memória Imunológica
[Mh] Termos MeSH secundário: Animais
Subpopulações de Linfócitos B/metabolismo
Linfócitos B/metabolismo
Antígeno B7-1/metabolismo
Centro Germinativo/imunologia
Switching de Imunoglobulina
Masculino
Camundongos
Plasmócitos/imunologia
Plasmócitos/metabolismo
Proteína 2 Ligante de Morte Celular Programada 1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7-1 Antigen); 0 (Programmed Cell Death 1 Ligand 2 Protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183877


  4 / 4746 MEDLINE  
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[PMID]:28631301
[Au] Autor:Laurent L; Le Fur A; Bloas RL; Néel M; Mary C; Moreau A; Poirier N; Vanhove B; Fakhouri F
[Ad] Endereço:INSERM UMR 1064, Nantes, France.
[Ti] Título:Prevention of lupus nephritis development in NZB/NZW mice by selective blockade of CD28.
[So] Source:Eur J Immunol;47(8):1368-1376, 2017 Aug.
[Is] ISSN:1521-4141
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Systemic lupus erythematosus (SLE) is a chronic systemic inflammatory disease. Autoantibodies (autoAbs) against double-stranded DNA (ds DNA), the hallmark of lupus, are produced and maintained by the interaction between auto-reactive B cells and CD4 T cells. This interplay is controlled by the CD28/CD80-86/CTLA-4 axis. Here we investigated whether selective blockade of CD28-CD80/86 co-stimulatory interactions abrogates lupus nephritis development in a murine model of SLE. To this aim, NZB/NZW F1 mice were treated for 3 months, either with an anti-CD28 Fab' fragment or a control Fab'-IgG. The effect of CD28 blockade on lupus nephritis onset, survival, production of anti-ds DNA antibodies and costimulatory molecules was evaluated. CD28 blockade prevented the development of lupus nephritis and prolonged survival during the 3-month treatment and 12 weeks after. Furthermore, the production of anti-ds DNA autoAbs was decreased. Lastly, the protective effect of CD28 blockade was associated with increased intrarenal expression of the immunoregulatory molecule, Indoleamine 2, 3-dioxygenase, of the co-inhibitory receptor programmed cell-Death - 1 (PD-1) and of its ligand programmed death ligand - 1 (PDL-1).In conclusion, CD28 blockade prevented the development of lupus nephritis in NZB/NZW F1 mice. This immunomodulatory strategy is a promising candidate for SLE therapy in humans.
[Mh] Termos MeSH primário: Anticorpos Antinucleares/sangue
Autoanticorpos/sangue
Antígenos CD28/antagonistas & inibidores
Imunoterapia/métodos
Nefrite Lúpica/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Anticorpos Antinucleares/imunologia
Autoanticorpos/imunologia
Linfócitos B/imunologia
Antígeno B7-1/antagonistas & inibidores
Antígeno B7-1/imunologia
Antígeno B7-1/metabolismo
Antígeno B7-2/antagonistas & inibidores
Antígeno B7-2/imunologia
Antígeno B7-2/metabolismo
Antígenos CD28/imunologia
Linfócitos T CD4-Positivos/imunologia
Modelos Animais de Doenças
Feminino
Fragmentos Fab das Imunoglobulinas/administração & dosagem
Indolamina-Pirrol 2,3,-Dioxigenase/genética
Indolamina-Pirrol 2,3,-Dioxigenase/imunologia
Rim/imunologia
Rim/patologia
Lúpus Eritematoso Sistêmico/imunologia
Nefrite Lúpica/imunologia
Camundongos
Camundongos Endogâmicos NZB
Receptor de Morte Celular Programada 1/genética
Receptor de Morte Celular Programada 1/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Antinuclear); 0 (Autoantibodies); 0 (B7-1 Antigen); 0 (B7-2 Antigen); 0 (CD28 Antigens); 0 (Cd86 protein, mouse); 0 (Immunoglobulin Fab Fragments); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 0 (Pdcd1 protein, mouse); 0 (Programmed Cell Death 1 Receptor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1002/eji.201746923


  5 / 4746 MEDLINE  
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[PMID]:28584006
[Au] Autor:Tesfagiorgis Y; Zhu SL; Jain R; Kerfoot SM
[Ad] Endereço:Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada.
[Ti] Título:Activated B Cells Participating in the Anti-Myelin Response Are Excluded from the Inflamed Central Nervous System in a Model of Autoimmunity that Allows for B Cell Recognition of Autoantigen.
[So] Source:J Immunol;199(2):449-457, 2017 Jul 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Once activated, T cells gain the ability to access both healthy and inflamed nonlymphoid tissues. They are then reactivated to remain in the tissue and exert their effector function only if they encounter their specific Ag. In this study, we set out to determine if the same is true for B cells using a mouse model of CNS autoimmunity that incorporates both T and B cell recognition of a myelin autoantigen. Both T and B cells were common infiltrates of spinal cords in diseased mice. However, unlike T cells, anti-myelin B cells were excluded from the inflamed tissue. Further, CNS B cells did not have a phenotype consistent with Ag-specific activation as it occurs in lymphatic tissue. Instead, they expressed elevated levels of CD80, indicating that B cells may contribute to local inflammation through nonantigen-specific mechanisms.
[Mh] Termos MeSH primário: Autoantígenos/imunologia
Linfócitos B/imunologia
Sistema Nervoso Central/imunologia
Encefalomielite Autoimune Experimental/imunologia
Ativação Linfocitária
Bainha de Mielina/imunologia
[Mh] Termos MeSH secundário: Animais
Autoimunidade
Linfócitos B/fisiologia
Antígeno B7-1/genética
Antígeno B7-1/imunologia
Movimento Celular/imunologia
Modelos Animais de Doenças
Inflamação/imunologia
Tecido Linfoide/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Medula Espinal/imunologia
Medula Espinal/patologia
Linfócitos T/imunologia
Linfócitos T/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantigens); 0 (B7-1 Antigen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1602042


  6 / 4746 MEDLINE  
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[PMID]:28527905
[Au] Autor:He X; Gong P; Wei Z; Liu W; Wang W; Li J; Yang Z; Zhang X
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun 130062, China.
[Ti] Título:Peroxisome proliferator-activated receptor-γ-mediated polarization of macrophages in Neospora caninum infection.
[So] Source:Exp Parasitol;178:37-44, 2017 Jul.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Neospora caninum is an apicomplexan parasite closely related Toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. Macrophage polarization plays an important role in host immune responses to parasites infection, such as Toxoplasma gondii, Leishmania, Trypanosoma cruzi. However, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during N. caninum infection remains unclear. METHODS: The M1 and M2-phenotypic markers of peritoneal macrophages from mice infected with tachyzoites of Nc-1 were analyzed by flow cytometry (FCM) analysis. Then J774A.1 cells were respectively treated with GW9662 and RGZ, and stimulated by tachyzoites of Nc-1. M1 and M2-phenotypic markers were determined by FCM and ELISA. And the activations of PPAR-γ and NF-κB were determined by Western blotting. RESULTS: In this study, our data showed that macrophages were preferentially differentiated into the M1 type during the acute stage of N. caninum infection, while the level of M2 macrophages significantly increased during the chronic stage of infection. In vitro study, compared with the GW9662 group and RGZ group, N. caninum can promote M2-polarized phenotype through up-regulate the activity of PPAR-γ and inhibting NF-κB activation. CONCLUSION: In conclusion, this study demonstrated that macrophages are plastic since M1 differentiated macrophages can express M2 markers with N. caninum infection through up-regulating the activity of PPAR-γ and inhibting NF-κB activation and may be providing new insights for the prevention and treatment of N. caninum infection.
[Mh] Termos MeSH primário: Coccidiose/parasitologia
Macrófagos Peritoneais/parasitologia
Neospora/fisiologia
PPAR gama/fisiologia
[Mh] Termos MeSH secundário: Animais
Arginase/metabolismo
Antígeno B7-1/metabolismo
Linhagem Celular
Cercopithecus aethiops
Coccidiose/metabolismo
Coccidiose/patologia
Citocinas/metabolismo
Citometria de Fluxo
Interleucina-10/metabolismo
Lectinas Tipo C/metabolismo
Ativação de Macrófagos
Macrófagos Peritoneais/imunologia
Lectinas de Ligação a Manose/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
NF-kappa B/metabolismo
Receptores de Superfície Celular/metabolismo
Transdução de Sinais
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7-1 Antigen); 0 (Cytokines); 0 (Lectins, C-Type); 0 (Mannose-Binding Lectins); 0 (NF-kappa B); 0 (PPAR gamma); 0 (Receptors, Cell Surface); 0 (mannose receptor); 130068-27-8 (Interleukin-10); EC 3.5.3.1 (Arg1 protein, mouse); EC 3.5.3.1 (Arginase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170522
[St] Status:MEDLINE


  7 / 4746 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:28513775
[Au] Autor:Xiao Y; Deng T; Shang Z; Wang D
[Ad] Endereço:Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
[Ti] Título:Adiponectin inhibits oxidization-induced differentiation of T helper cells through inhibiting costimulatory CD40 and CD80.
[So] Source:Braz J Med Biol Res;50(6):e6227, 2017 May 15.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Adiponectin is a multifunctional adipokine that has several oligomeric forms in the blood stream, which broadly regulates innate and acquired immunity. Therefore, in this study, we aimed to observe the differentiation of T helper (Th) cells and expression of costimulatory signaling molecules affected by adiponectin. The mRNA and protein expression levels of adiponectin and its receptors in oxidized low density lipoprotein cholesterol-treated endothelial cells were assayed by real time PCR and immunofluorescence. The endothelial cells were then treated with adiponectin with or without adipoR1 or adipoR2 siRNA and co-cultured with T lymphocytes. The distribution of Th1, Th2 and Th17 subsets were assayed by flow cytometry. The effects of adiponectin on costimulatory signaling molecules HLA-DR, CD80, CD86 and CD 40 was also assayed by flow cytometry. The results showed that endothelial cells expressed adiponectin and its receptor adipoR1 and adipoR2, but not T-cadherin. Adiponectin suppressed Th1 and Th17 differentiation through adipoR1 receptor, contributed to the inhibition of CD80 and CD40, and inhibited differentiation of Th1 and Th17 by inhibiting antigen presenting action.
[Mh] Termos MeSH primário: Adiponectina/metabolismo
Antígeno B7-1/metabolismo
Antígenos CD40/metabolismo
Linfócitos T Auxiliares-Indutores/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adiponectina/genética
Adiponectina/farmacologia
Adulto
Diferenciação Celular
Células Cultivadas
Células Endoteliais/efeitos dos fármacos
Células Endoteliais/metabolismo
Antígenos HLA-DR/metabolismo
Células Endoteliais da Veia Umbilical Humana/citologia
Seres Humanos
Recém-Nascido
Lipoproteínas LDL/farmacologia
Receptores de Adiponectina/efeitos dos fármacos
Receptores de Adiponectina/metabolismo
Linfócitos T Auxiliares-Indutores/citologia
Linfócitos T Auxiliares-Indutores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ADIPOR1 protein, human); 0 (ADIPOR2 protein, human); 0 (Adiponectin); 0 (B7-1 Antigen); 0 (CD40 Antigens); 0 (HLA-DR Antigens); 0 (Lipoproteins, LDL); 0 (Receptors, Adiponectin); 0 (oxidized low density lipoprotein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE


  8 / 4746 MEDLINE  
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Antunes, Edson
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[PMID]:28489854
[Au] Autor:Braga FG; Ruas LP; Pereira RM; Lima XT; Antunes E; Mamoni RL; Blotta MHSL
[Ad] Endereço:Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Campinas, São Paulo, Brazil.
[Ti] Título:Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis.
[So] Source:PLoS Negl Trop Dis;11(5):e0005601, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Eosinophilia is a typical finding of the acute/juvenile form of paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America. This clinical form is characterized by depressed cellular immune response and production of Th2 cytokines. Moreover, it has been shown that the increased number of eosinophils in peripheral blood of patients returns to normal values after antifungal treatment. However, the role of eosinophils in PCM has never been evaluated. This study aimed to assess the phenotypic and functional characteristics of eosinophils in PCM. METHODS/PRINCIPAL FINDINGS: In 15 patients with the acute form of the disease, we detected expression of MBP, CCL5 (RANTES) and CCL11 (eotaxin) in biopsies of lymph nodes and liver. In addition, there were higher levels of chemokines and granule proteins in the peripheral blood of patients compared to controls. Isolation of eosinophils from blood revealed a higher frequency of CD69+ and TLR2+ eosinophils in patients compared to controls, and a lower population of CD80+ cells. We also evaluated the fungicidal capacity of eosinophils in vitro. Our results revealed that eosinophils from PCM patients and controls exhibit similar ability to kill P. brasiliensis yeast cells, although eosinophils of patients were less responsive to IL-5 stimulation than controls. CONCLUSION/PRINCIPAL FINDINGS: In conclusion, we suggest that eosinophils might play a role in the host response to fungi and in the pathophysiology of PCM by inducing an intense and systemic inflammatory response in the initial phase of the infection.
[Mh] Termos MeSH primário: Eosinofilia/patologia
Eosinófilos/imunologia
Paracoccidioides/imunologia
Paracoccidioidomicose/complicações
Paracoccidioidomicose/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antígenos CD/análise
Antígenos de Diferenciação de Linfócitos T/análise
Antígeno B7-1/análise
Criança
Pré-Escolar
Citocinas/sangue
Eosinófilos/química
Feminino
Seres Humanos
Lectinas Tipo C/análise
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (B7-1 Antigen); 0 (CD69 antigen); 0 (Cytokines); 0 (Lectins, C-Type)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005601


  9 / 4746 MEDLINE  
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[PMID]:28439878
[Au] Autor:Brunk F; Michel C; Holland-Letz T; Slynko A; Kopp-Schneider A; Kyewski B; Pinto S
[Ad] Endereço:Division of Developmental Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
[Ti] Título:Dissecting and modeling the emergent murine TEC compartment during ontogeny.
[So] Source:Eur J Immunol;47(7):1153-1159, 2017 Jul.
[Is] ISSN:1521-4141
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The origin of the thymic epithelium, i.e. the cortical (cTEC) and medullary (mTEC) epithelial cells, from bipotent stem cells through TEC progenitors and lineage-specific progeny still remains poorly understood. We sought to obtain an unbiased view of the incipient emergence of TEC subsets by following embryonic TEC development based on co-expression of EpCAM, CD80 and MHC class II (MHCII) on non-hematopoietic (CD45 ) thymic stromal cells in wild-type BL6 mice. Using a combination of ex vivo analysis, Re-aggregate Thymic Organ Culture (RTOC) reconstitution assays and mathematical modeling, we traced emergent lineage commitment in murine embryonic TECs. Both experimental and mathematical datasets supported the following developmental sequence: MHCII CD80 → MHCII CD80 → MHCII CD80 → MHCII CD80 TECs, whereby MHCII CD80 and MHCII CD80 TECs bear features of cTECs and mTECs respectively. These emergent MHCII CD80 cTECs directly generate mature MHCII CD80 mTECs in vivo and in vitro, thus supporting the asynchronous model of TEC lineage commitment.
[Mh] Termos MeSH primário: Diferenciação Celular
Células Epiteliais/fisiologia
Timócitos/fisiologia
Timo/citologia
[Mh] Termos MeSH secundário: Animais
Antígeno B7-1/genética
Antígeno B7-1/imunologia
Linhagem da Célula
Células Cultivadas
Molécula de Adesão da Célula Epitelial/genética
Molécula de Adesão da Célula Epitelial/imunologia
Células Epiteliais/imunologia
Expressão Gênica
Genes MHC Classe II/genética
Genes MHC Classe II/imunologia
Antígenos Comuns de Leucócito/deficiência
Antígenos Comuns de Leucócito/genética
Antígenos Comuns de Leucócito/imunologia
Camundongos
Modelos Teóricos
Técnicas de Cultura de Órgãos
Timo/embriologia
Timo/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7-1 Antigen); 0 (Epithelial Cell Adhesion Molecule); EC 3.1.3.48 (Leukocyte Common Antigens); EC 3.1.3.48 (Ptprc protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1002/eji.201747006


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[PMID]:28414296
[Au] Autor:Ni X; Song Q; Cassady K; Deng R; Jin H; Zhang M; Dong H; Forman S; Martin PJ; Chen YZ; Wang J; Zeng D
[Ti] Título:PD-L1 interacts with CD80 to regulate graft-versus-leukemia activity of donor CD8+ T cells.
[So] Source:J Clin Invest;127(5):1960-1977, 2017 May 01.
[Is] ISSN:1558-8238
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Programmed death ligand-1 (PD-L1) interacts with programmed death-1 (PD-1) and the immunostimulatory molecule CD80 and functions as a checkpoint to regulate immune responses. The interaction of PD-L1 with CD80 alone has been shown to exacerbate the severity of graft-versus-host disease (GVHD), whereas costimulation of CD80 and PD-1 ameliorates GVHD. Here we have demonstrated that temporary depletion of donor CD4+ T cells early after hematopoietic cell transplantation effectively prevents GVHD while preserving strong graft-versus-leukemia (GVL) effects in allogeneic and xenogeneic murine GVHD models. Depletion of donor CD4+ T cells increased serum IFN-γ but reduced IL-2 concentrations, leading to upregulation of PD-L1 expression by recipient tissues and donor CD8+ T cells. In GVHD target tissues, the interactions of PD-L1 with PD-1 on donor CD8+ T cells cause anergy, exhaustion, and apoptosis, thereby preventing GVHD. In lymphoid tissues, the interactions of PD-L1 with CD80 augment CD8+ T cell expansion without increasing anergy, exhaustion, or apoptosis, resulting in strong GVL effects. These results indicate that the outcome of PD-L1-mediated signaling in CD8+ T cells depends on the presence or absence of CD4+ T cells, the nature of the interacting receptor expressed by CD8+ T cells, and the tissue environment in which the signaling occurs.
[Mh] Termos MeSH primário: Antígeno B7-1/imunologia
Antígeno B7-H1/imunologia
Linfócitos T CD8-Positivos/imunologia
Efeito Enxerto vs Leucemia/imunologia
Transdução de Sinais/imunologia
[Mh] Termos MeSH secundário: Animais
Antígeno B7-1/genética
Antígeno B7-H1/genética
Linfócitos T CD4-Positivos/imunologia
Doença Enxerto-Hospedeiro/genética
Doença Enxerto-Hospedeiro/imunologia
Efeito Enxerto vs Leucemia/genética
Interferon gama/genética
Interferon gama/imunologia
Interleucina-2/genética
Interleucina-2/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Transgênicos
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7-1 Antigen); 0 (B7-H1 Antigen); 0 (Cd274 protein, mouse); 0 (IFNG protein, mouse); 0 (Interleukin-2); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE



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