Base de dados : MEDLINE
Pesquisa : D12.776.543.695.750 [Categoria DeCS]
Referências encontradas : 1326 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 133 ir para página                         

  1 / 1326 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29458554
[Au] Autor:Osaka S; Okuzumi K; Koide S; Tamai K; Sato T; Tanimoto K; Tomita H; Suzuki M; Nagano Y; Shibayama K; Arakawa Y; Nagano N
[Ad] Endereço:1​Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, Nagano, Japan.
[Ti] Título:Genetic shifts in methicillin-resistant Staphylococcus aureus epidemic clones and toxin gene profiles in Japan: comparative analysis among pre-epidemic, epidemic and post-epidemic phases.
[So] Source:J Med Microbiol;67(3):392-399, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The decline in methicillin-resistant Staphylococcus aureus (MRSA) isolation rates has become a general observation worldwide, including Japan. We hypothesized that some genetic shift in MRSA might cause this phenomenon, and therefore we investigated the genetic profiles among MRSA clinical isolates obtained from three different epidemic phases in Japan. METHODOLOGY: A total of 353 MRSA isolates were selected from 202 medical facilities in 1990 (pre-epidemic phase), 2004 (epidemic phase) and 2016 (post-epidemic phase). Molecular typing was performed by PCR detection of 22 genes using the polymerase chain reaction (PCR)-based ORF typing (POT) system, including an additional eight genes including small genomic islets and seven toxin genes. RESULTS: Isolates with a POT1 of score 93, identified as presumed clonal complex (pCC)5-staphylococcal cassette chromosome mec (SCCmec) type II including ST5-SCCmec type II New York/Japan clone, represented the major epidemic MRSA lineage in 1990 and 2004. In 2016, however, a marked decrease in isolates with a POT1 score of 93, along with changes in the epidemiology of toxin genes carried, was noted, where the carriers of tst genes including the tst-sec combination were markedly reduced, and those possessing the seb gene alone were markedly increased. Rather, isolates with a POT1 score of 106, including pCC1 or pCC8 among the isolates with SCCmec type IV, which often links to community-associated MRSA, were predominant. Interestingly, the pCC1 and pCC8 lineages were related to sea and tst-sec carriage, respectively. CONCLUSIONS: Over time, a transition in MRSA genetic profiles from a POT1 score of 93 in 1990 and 2004 to 106 in 2014 was found in Japan.
[Mh] Termos MeSH primário: Toxinas Bacterianas/genética
Epidemias
Deriva Genética
Staphylococcus aureus Resistente à Meticilina/genética
Infecções Estafilocócicas/epidemiologia
Infecções Estafilocócicas/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Infecção Hospitalar/epidemiologia
Exotoxinas/genética
Seres Humanos
Japão/epidemiologia
Leucocidinas/genética
Meticilina/farmacologia
Resistência a Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Tipagem Molecular
Fatores de Virulência/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Virulence Factors); Q91FH1328A (Methicillin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000687


  2 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29179212
[Au] Autor:Sun XX; Zhang SS; Dai CY; Peng J; Pan Q; Xu LF; Ma XL
[Ad] Endereço:Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei, China.
[Ti] Título:LukS-PV-Regulated MicroRNA-125a-3p Promotes THP-1 Macrophages Differentiation and Apoptosis by Down-Regulating NF1 and Bcl-2.
[So] Source:Cell Physiol Biochem;44(3):1093-1105, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: LukS-PV is a component of Panton-Valentine leukocidin (PVL). We have previously demonstrated that LukS-PV potently promoted differentiation and induced apoptosis in THP-1 cells. However, the precise mechanisms of these actions remain unknown. MicroRNAs (miRs) play important roles in cellular differentiation and apoptosis. This study aimed to investigate the role of miR-125a-3p in LukS-PV-regulated differentiation and apoptosis and its underlying mechanism in THP-1 cells. METHODS: MicroRNA profiling analyses were conducted to determine differential miRNA expression levels in THP-1 cells treated with LukS-PV. Cell differentiation and apoptosis were measured in THP-1 cells by gain-of-function and loss-of-function experiments. Bioinformatics analysis and luciferase reporter assays were used to confirm the targets of miR-125a-3p. The effects of the miR-125a-3p targets on cellular differentiation were determined by knocking them down. RESULTS: MiR-125a-3p was up-regulated after treating the human monocytic leukaemia cell line THP-1 with LukS-PV. In addition, miR-125a-3p positively regulated apoptosis and differentiation in THP-1 cells treated with LukS-PV. Concordantly, luciferase reporter assays confirmed that neurofibromatosis type 1 (NF1) and B-cell lymphoma 2 (Bcl-2) were direct target genes of miR-125a-3p. Moreover, NF1 knockdown in THP-1 cells significantly promoted differentiation in vitro. Finally, the extracellular signal-regulated kinase (ERK) pathway, a downstream target of NF1, was activated after NF1 knockdown. CONCLUSIONS: These findings confirm that miR-125a-3p is involved in LukS-PV-mediated cell differentiation and apoptosis in THP-1 cells.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Toxinas Bacterianas/farmacologia
Diferenciação Celular/efeitos dos fármacos
Exotoxinas/farmacologia
Leucocidinas/farmacologia
MicroRNAs/metabolismo
Neurofibromina 1/metabolismo
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Antagomirs/metabolismo
Toxinas Bacterianas/genética
Toxinas Bacterianas/metabolismo
Sequência de Bases
Caspase 3/metabolismo
Linhagem Celular
Regulação para Baixo/efeitos dos fármacos
Exotoxinas/genética
Exotoxinas/metabolismo
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Seres Humanos
Leucocidinas/genética
Leucocidinas/metabolismo
Macrófagos/citologia
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
MicroRNAs/antagonistas & inibidores
MicroRNAs/genética
Neurofibromina 1/antagonistas & inibidores
Neurofibromina 1/genética
Fosforilação/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-bcl-2/antagonistas & inibidores
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/farmacologia
Alinhamento de Sequência
Transdução de Sinais/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (Antagomirs); 0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (MIRN125 microRNA, human); 0 (MicroRNAs); 0 (Neurofibromin 1); 0 (Panton-Valentine leukocidin); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Recombinant Proteins); 0 (bcl-2-Associated X Protein); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1159/000485415


  3 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29261764
[Au] Autor:Leistner R; Kola A; Gastmeier P; Krüger R; Hoppe PA; Schneider-Burrus S; Zuschneid I; Wischnewski N; Bender J; Layer F; Niebank M; Scheibenbogen C; Hanitsch LG
[Ad] Endereço:Institute of Hygiene and Environmental Medicine, Charité Universitätsmedizin Berlin, Berlin, Germany.
[Ti] Título:Pyoderma outbreak among kindergarten families: Association with a Panton-Valentine leukocidin (PVL)-producing S. aureus strain.
[So] Source:PLoS One;12(12):e0189961, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: We report on an outbreak of skin and soft tissue infections (SSTI) among kindergarten families. We analyzed the transmission route and aimed to control the outbreak. METHODS: The transmission route was investigated by nasal screening for Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus (PVL-SA), subsequent microbiological investigation including whole genome sequencing and a questionnaire-based analysis of epidemiological information. The control measures included distribution of outbreak information to all individuals at risk and implementation of a Staphylococcus aureus decontamination protocol. RESULTS: Individuals from 7 of 19 families were either colonized or showed signs of SSTI such as massive abscesses or eye lid infections. We found 10 PVL-SA isolates in 9 individuals. In the WGS-analysis all isolates were found identical with a maximum of 17 allele difference. The clones were methicillin-susceptible but cotrimoxazole resistant. In comparison to PVL-SAs from an international strain collection, the outbreak clone showed close genetical relatedness to PVL-SAs from a non-European country. The questionnaire results showed frequent travels of one family to this area. The results also demonstrated likely transmission via direct contact between families. After initiation of Staphylococcus aureus decontamination no further case was detected. CONCLUSIONS: Our outbreak investigation showed the introduction of a PVL-SA strain into a kindergarten likely as a result of international travel and further transmission by direct contact. The implementation of a Staphylococcus aureus decontamination protocol was able to control the outbreak.
[Mh] Termos MeSH primário: Toxinas Bacterianas/biossíntese
Surtos de Doenças/estatística & dados numéricos
Exotoxinas/biossíntese
Leucocidinas/biossíntese
Pioderma/epidemiologia
Pioderma/microbiologia
Infecções Estafilocócicas/epidemiologia
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/fisiologia
[Mh] Termos MeSH secundário: Alelos
Pré-Escolar
Família
Seres Humanos
Funções Verossimilhança
Filogenia
Apoio Social
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189961


  4 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28854219
[Au] Autor:Shekarabi M; Hajikhani B; Salimi Chirani A; Fazeli M; Goudarzi M
[Ad] Endereço:Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:Molecular characterization of vancomycin-resistant Staphylococcus aureus strains isolated from clinical samples: A three year study in Tehran, Iran.
[So] Source:PLoS One;12(8):e0183607, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Emergence of vancomycin-intermediate Staphylococcus aureus (VISA) and vancomycin-resistant S. aureus (VRSA) strains has led to great concern in global public health in both developing and developed countries. This study investigated distribution and molecular characterization of VRSA strains in Tehran's hospitals using a combination of molecular typing methods. MATERIALS AND METHODS: A total of 1789 S. aureus isolates obtained between 2014 and 2017 and were characterized using antibiogram, SCCmec typing, spa typing, and multilocus-sequence typing. Resistance to vancomycin was determined by E-test method. After confirmation of the isolated VRSA strain, genetic analysis was performed by evaluating vanA and vanB genes presence.The presence of resistance (ermA, ermB, ermC, mupA, msrA, msrB, tetM, ant (4΄)-Ia, aac (6΄)-Ie/aph (2˝), aph (3΄)-IIIa) and toxin (etb, eta, pvl, tst) encoding genes was investigated by the polymerase chain reaction (PCR) technique. RESULTS: Of all S. aureus tested isolates, four isolates were confirmed as VRSA isolates and two isolates confirmed as VISA isolates. ST5- SCCmec II/t002 and ST239-SCCmec III/t037 strains had MIC values of 512µg/ml, ST239-SCCmec III/t037 and ST8-SCCmecIV/t008 strains had MIC values of 64µg/ml and ST22-SCCmec IV/t790 and ST239-SCCmec III/t030 strains had MIC values ≥ 8 µg/ml. pvl-encoding gene was confirmed in ST8-SCCmecIV/t008 and ST22-SCCmec IV/t790 strains. The isolates differed in the carriage of resistance and toxin encoding genes. CONCLUSIONS: The study revealed the existence of VRSA strains in capital of Iran, Tehran. To our knowledge, this is the first report of ST239-SCCmec III/t037 as VRSA strain. These findings support the need for future surveillance studies on VRSA strains to keep the emergence and transmission of these isolates to a minimum.
[Mh] Termos MeSH primário: Infecção Hospitalar/microbiologia
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/genética
Resistência a Vancomicina/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Toxinas Bacterianas/genética
Técnicas de Tipagem Bacteriana/métodos
Carbono-Oxigênio Ligases/genética
Infecção Hospitalar/epidemiologia
Exotoxinas/genética
Genes Bacterianos/genética
Hospitais
Seres Humanos
Irã (Geográfico)/epidemiologia
Leucocidinas/genética
Testes de Sensibilidade Microbiana/métodos
Proteínas Nucleares/genética
Proteínas de Ligação às Penicilinas/genética
Reação em Cadeia da Polimerase
Infecções Estafilocócicas/epidemiologia
Staphylococcus aureus/classificação
Staphylococcus aureus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Nuclear Proteins); 0 (Panton-Valentine leukocidin); 0 (Penicillin-Binding Proteins); 0 (VanA ligase, Bacteria); 0 (mecA protein, Staphylococcus aureus); 0 (mupA protein, Staphylococcus aureus); EC 6.1.- (Carbon-Oxygen Ligases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183607


  5 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28816638
[Au] Autor:Rojas I; Barquero-Calvo E; van Balen JC; Rojas N; Muñoz-Vargas L; Hoet AE
[Ad] Endereço:1 Programa de Investigación en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional , Heredia, Costa Rica .
[Ti] Título:High Prevalence of Multidrug-Resistant Community-Acquired Methicillin-Resistant Staphylococcus aureus at the Largest Veterinary Teaching Hospital in Costa Rica.
[So] Source:Vector Borne Zoonotic Dis;17(9):645-653, 2017 Sep.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen associated with severe infections in companion animals present in the community, and it is diagnosed in animals admitted to veterinary hospitals. However, reports that describe the circulation of MRSA in animal populations and veterinary settings in Latin America are scarce. Therefore, the objective of this study was to determine the prevalence and investigate the molecular epidemiology of MRSA in the environment of the largest veterinary teaching hospital in Costa Rica. Preselected contact surfaces were sampled twice within a 6-week period. Antimicrobial resistance, SCCmec type, Panton-Valentine leukocidin screening, USA type, and clonality were assessed in all recovered isolates. Overall, MRSA was isolated from 26.5% (27/102) of the surfaces sampled, with doors, desks, and examination tables most frequently contaminated. Molecular analysis demonstrated a variety of surfaces from different sections of the hospital contaminated by three highly related clones/pulsotypes. All, but one of the isolates were characterized as multidrug-resistant SCCmec type IV-USA700, a strain sporadically described in other countries and often classified as community acquired. The detection and frequency of this unique strain in this veterinary setting suggest Costa Rica has a distinctive MRSA ecology when compared with other countries/regions. The high level of environmental contamination highlights the necessity to establish and enforce standard cleaning and disinfection protocols to minimize further spread of this pathogen and reduce the risk of nosocomial and/or occupational transmission of MRSA.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Microbiologia Ambiental
Hospitais Veterinários
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Toxinas Bacterianas/metabolismo
Técnicas de Tipagem Bacteriana/veterinária
Costa Rica
Estudos Transversais
Contaminação de Equipamentos
Exotoxinas/metabolismo
Regulação Bacteriana da Expressão Gênica
Hospitais de Ensino
Leucocidinas/metabolismo
Staphylococcus aureus Resistente à Meticilina/genética
Proteínas de Ligação às Penicilinas/genética
Proteínas de Ligação às Penicilinas/metabolismo
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin); 0 (Penicillin-Binding Proteins); 0 (mecA protein, Staphylococcus aureus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2017.2145


  6 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28679429
[Au] Autor:Wang HK; Huang CY; Huang YC
[Ad] Endereço:School of Medicine, Chang Gung University, Gueishan, Taoyuan, Taiwan.
[Ti] Título:Clinical features and molecular characteristics of childhood community-associated methicillin-resistant Staphylococcus aureus infection in a medical center in northern Taiwan, 2012.
[So] Source:BMC Infect Dis;17(1):470, 2017 Jul 05.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Since first reported in 2002, the rate of methicillin-resistant Staphylococcus aureus (MRSA) among childhood community-associated (CA) S. aureus infection in Taiwan increased significantly up to 2005. There have been no reports on this issue since then. METHODS: We prospectively collected clinical S. aureus isolates from the patients <19 years of age in a university-affiliated hospital in 2012. Only first isolate from each patient was included. The medical records were retrospectively reviewed and the patients were classified as CA or healthcare-associated (HA) by the standard epidemiologic criteria. Isolates as CA-MRSA were further characterized by pulsed-field gel electrophoresis, staphylococcal cassette chromosome (SCCmec) typing, and multilocus sequence typing. RESULTS: A total of 409 S. aureus isolates were included, and 260 (63.6%) were MRSA. The proportion of MRSA among all S. aureus isolates in 2012 increased significantly (p < 0.001) compared to that in 2004-2005. Of the 181 CA-MRSA isolates, 86.2% were identified from pus or wound. Nine pulsotypes were identified with two major types (type D, 119 (65.7%); type C, 27 (14.9%). Most of the isolates carried either SCCmec IV (66 isolates, 36%) or V (112 isolates, 62%). 128 isolates (71%) carried Panton-Valentine leukocidin (PVL) genes. Clonal complex (CC) 59 accounted for 146 isolates (80.7%) of two major pulsotypes, CC45 for 19 isolates, ST30 for 6 isolates and ST8 (USA 300) for 4 isolates. In addition to penicillin (100%), most isolates were resistant to erythromycin (81%) and clindamycin (79.3%). CONCLUSIONS: Around two-thirds of childhood community-associated S. aureus infections in northern Taiwan were MRSA. Though CC59 is still the prevalent community clone, several new clones emerged in northern Taiwan.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Infecções Comunitárias Adquiridas/microbiologia
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Infecções Estafilocócicas/etiologia
[Mh] Termos MeSH secundário: Adolescente
Toxinas Bacterianas/genética
Criança
Pré-Escolar
Clindamicina/farmacologia
Infecções Comunitárias Adquiridas/epidemiologia
Eletroforese em Gel de Campo Pulsado
Eritromicina/farmacologia
Exotoxinas/genética
Feminino
Hospitais
Seres Humanos
Lactente
Leucocidinas/genética
Masculino
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/genética
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
Estudos Retrospectivos
Taiwan/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin); 3U02EL437C (Clindamycin); 63937KV33D (Erythromycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2560-0


  7 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28640000
[Au] Autor:Green K; Chranioti I; Singh S; Jäger HR; Drebes A; Gabbie S; Cohen J
[Ad] Endereço:From the *Department of Paediatrics, Royal Free London NHS Foundation Trust, †Paediatric & Adolescent Division, University College London Hospitals NHS Foundation Trust, ‡Centre of Medical Imaging, University College London, §UCL Institute of Neurology Academic Neuroradiological Unit, University College London, and ¶KD Haemophilia and Thrombosis Centre, Royal Free London NHS Foundation Trust, London, United Kingdom.
[Ti] Título:Panton-Valentine Leukocidin Producing Staphylococcus Aureus Facial Pyomyositis Causing Partial Cavernous Sinus Thrombosis.
[So] Source:Pediatr Infect Dis J;36(11):1102-1104, 2017 Nov.
[Is] ISSN:1532-0987
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We present a case of subtotal cavernous sinus thrombosis secondary to Panton-Valentine leukocidin-associated Staphylococcus aureus pyomyositis of the muscles of mastication in a previously healthy child, who was successfully managed with no residual disease. He was found to have a factor V Leiden heterozygous mutation. We highlight the propensity of Panton-Valentine leukocidin Staphylococcus aureus to induce venous thrombosis at any site but with potential for more severe consequences in the head. We highlight pyomyositis as a differential for periorbital cellulitis and discuss the significance of the factor V Leiden mutation.
[Mh] Termos MeSH primário: Trombose do Corpo Cavernoso
Piomiosite
Infecções Estafilocócicas
Staphylococcus aureus
[Mh] Termos MeSH secundário: Adolescente
Toxinas Bacterianas
Trombose do Corpo Cavernoso/diagnóstico por imagem
Trombose do Corpo Cavernoso/etiologia
Exotoxinas
Cabeça/diagnóstico por imagem
Seres Humanos
Leucocidinas
Imagem por Ressonância Magnética
Masculino
Piomiosite/complicações
Piomiosite/diagnóstico por imagem
Piomiosite/microbiologia
Infecções Estafilocócicas/complicações
Infecções Estafilocócicas/diagnóstico por imagem
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/isolamento & purificação
Staphylococcus aureus/patogenicidade
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1097/INF.0000000000001667


  8 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28610560
[Au] Autor:Asiimwe BB; Baldan R; Trovato A; Cirillo DM
[Ad] Endereço:Emerging Bacterial Pathogens Unit, IRCCS, Via Olgettina 58, Milan, Italy. basiimwe@chs.mak.ac.ug.
[Ti] Título:Prevalence and molecular characteristics of Staphylococcus aureus, including methicillin resistant strains, isolated from bulk can milk and raw milk products in pastoral communities of South-West Uganda.
[So] Source:BMC Infect Dis;17(1):422, 2017 Jun 13.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Staphylococcus aureus strains are now regarded as zoonotic agents. In pastoral settings where human-animal interaction is intimate, multi-drug resistant microorganisms have become an emerging zoonotic issue of public health concern. The study of S. aureus prevalence, antimicrobial resistance and clonal lineages in humans, animals and food in African settings has great relevance, taking into consideration the high diversity of ethnicities, cultures and food habits that determine the lifestyle of the people. Little is known about milk carriage of methicillin resistant S. aureus strains (MRSA) and their virulence factors in Uganda. Here, we present the prevalence of MRSA in bulk can milk and raw milk products in pastoral communities of south-west Uganda. We also present PFGE profiles, spa-types, as well as frequency of enterotoxins genes. METHODS: S. aureus was identified by the coagulase test, susceptibility testing by the Kirby-Bauer disc diffusion and E-test methods and MRSA by detection of the mecA gene and SCCmec types. The presence of Panton - Valentine Leucocidin (PVL) genes and staphylococcal enterotoxins was determined by PCR, while genotyping was by PFGE and spa typing. RESULTS: S. aureus were isolated from 30/148 (20.3%) milk and 11/91(12%) sour milk samples. mecA gene carriage, hence MRSA, was detected in 23/41 (56.1%) of the isolates, with 21 of the 23 (91.3%) being SCCmec type V; while up to 30/41 (73.2%) of the isolates were resistant to tetracycline. Only five isolates carried the PVL virulence gene, while PFGE typing revealed ten clusters (ranging from two seven isolates each) that comprised 83% of the sample, and only eight isolates with unique pulsotypes. The largest PFGE profile (E) consisted of seven isolates while t7753, t1398, and t2112 were the most common spa-types. Thirty seven of the 41 strains (90.2%) showed at least one of the eight enterotoxin genes tested, with sem 29 (70.7%), sei 25 (61%) and seg 21 (51.2%) being the most frequently observed genes. CONCLUSION: This is the first study to demonstrate MRSA and enterotoxin genes in raw milk and its products in Uganda. The fact that over 90% of the isolates carried at least one gene encoding enterotoxins shows a high risk of spread of foodborne diseases through milk in this setting.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Microbiologia de Alimentos/estatística & dados numéricos
Leite/microbiologia
Staphylococcus aureus/genética
[Mh] Termos MeSH secundário: Animais
Toxinas Bacterianas/genética
Eletroforese em Gel de Campo Pulsado
Enterotoxinas/genética
Exotoxinas/genética
Doenças Transmitidas por Alimentos/microbiologia
Genótipo
Seres Humanos
Leucocidinas/genética
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Prevalência
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/isolamento & purificação
Uganda
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Toxins); 0 (Enterotoxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin); 0 (Virulence Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2524-4


  9 / 1326 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28592231
[Au] Autor:Harch SAJ; MacMorran E; Tong SYC; Holt DC; Wilson J; Athan E; Hewagama S
[Ad] Endereço:Alice Springs Hospital, Alice Springs, Northern Territory, Australia. susan.harch@sa.gov.au.
[Ti] Título:High burden of complicated skin and soft tissue infections in the Indigenous population of Central Australia due to dominant Panton Valentine leucocidin clones ST93-MRSA and CC121-MSSA.
[So] Source:BMC Infect Dis;17(1):405, 2017 Jun 07.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Superficial skin and soft tissue infections (SSTIs) are common among the Indigenous population of the desert regions of Central Australia. However, the overall burden of disease and molecular epidemiology of Staphylococcus aureus complicated SSTIs has yet to be described in this unique population. METHODS: Alice Springs Hospital (ASH) admission data was interrogated to establish the population incidence of SSTIs. A prospective observational study was conducted on a subset of S. aureus complicated SSTIs (carbuncles and furuncles requiring surgical intervention) presenting during a one month period to further characterize the clinical and molecular epidemiology. High resolution melting analysis was used for clonal complex discrimination. Real-time polymerase chain reaction identifying the lukF component of the Panton Valentine leucocidin (pvl) gene determined pvl status. Clinical and outcome data was obtained from the ASH medical and Northern Territory shared electronic health records. RESULTS: SSTIs represented 2.1% of ASH admissions during 2014. 82.6% occurred in Indigenous patients (n = 382) with an estimated incidence of 18.9 per 1, 000 people years compared to the non-Indigenous population of 2.9 per 1000, with an incident rate ratio of 6.6 (95% confidence interval 5.1-8.5). Clinical and molecular analysis was performed on 50 isolates from 47 patients. Community-associated methicillin-resistant S. aureus (CA-MRSA) predominated (57% of isolates). The high burden of SSTIs is partly explained by the prevalence of pvl positive strains of S. aureus (90% isolates) for both CA-MRSA and methicillin-susceptible S. aureus (MSSA). ST93-MRSA and CC121-MSSA were the most prevalent clones. SSTIs due to ST93-MRSA were more likely to require further debridement (p = 0.039), however they also more frequently received inactive antimicrobial therapy (p < 0.001). CONCLUSIONS: ST93-MRSA and CC121-MSSA are the dominant causes of carbuncles and furuncles in Central Australia. Both of these virulent clones harbor pvl but the impact on clinical outcomes remains uncertain. The high prevalence of CA-MRSA supports empiric vancomycin use in this population when antimicrobial therapy is indicated. Prompt surgical intervention remains the cornerstone of treatment.
[Mh] Termos MeSH primário: Toxinas Bacterianas/genética
Exotoxinas/genética
Leucocidinas/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Infecções dos Tecidos Moles/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Criança
Infecções Comunitárias Adquiridas/epidemiologia
Feminino
Seres Humanos
Masculino
Resistência a Meticilina
Staphylococcus aureus Resistente à Meticilina/genética
Meia-Idade
Epidemiologia Molecular
Northern Territory/epidemiologia
Grupos Populacionais/estatística & dados numéricos
Prevalência
Estudos Prospectivos
Pele/microbiologia
Infecções dos Tecidos Moles/epidemiologia
Infecções Estafilocócicas/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2460-3


  10 / 1326 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28533831
[Au] Autor:Samutela MT; Kalonda A; Mwansa J; Lukwesa-Musyani C; Mwaba J; Mumbula EM; Mwenya D; Simulundu E; Kwenda G
[Ad] Endereço:Department of Biomedical Sciences, School of Medicine, University of Zambia,P.O. Box 50110, Lusaka, Zambia.
[Ti] Título:Molecular characterisation of methicillin-resistant Staphylococcus aureus (MRSA) isolated at a large referral hospital in Zambia.
[So] Source:Pan Afr Med J;26:108, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Methicillin-resistant (MRSA) is globally recognized as an important public health problem. Whereas comprehensive molecular typing data of MRSA strains is available, particularly in Europe, North America and Australia, similar information is very limited in sub-Saharan Africa including Zambia. METHODS: In this study, thirty two clinical isolates of , collected at a large referral hospital in Lusaka, Zambia between June 2009 and December 2012 were analysed by Staphylococcal cassette chromosome protein A gene typing and detection of the Panton-Valentine Leukocidin genes . RESULTS: Three SCC types were identified namely SCC type IV (65.6%), SCCmec type III (21.9%), SCC type I (3.1%). Nine point four percent (9.4%) of the isolates were untypable. Five types, which included a novel type, were detected and the most prevalent spa type was t064 (40.6%). Other types included spa types t2104 (31.3%), t355 (3.1%) and t1257 (21.9%). The genes were detected in 3 out of 32 isolates. CONCLUSION: These molecular typing data indicated that the MRSA strains collected in Lusaka were diverse. Although the source of these MRSA was not established, these results stress the need for assessing infection prevention and control procedures at this health-care facility in order to curtail possible nosocomial infections. Furthermore, country-wide surveillance of MRSA in both the community and health-care facilities is recommended for infection prevention and control. To our knowledge, this represents the first study to characterise MRSA using molecular tools in Zambia.
[Mh] Termos MeSH primário: Genes Bacterianos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Infecções Estafilocócicas/epidemiologia
[Mh] Termos MeSH secundário: Toxinas Bacterianas/genética
Estudos Transversais
Exotoxinas/genética
Seres Humanos
Leucocidinas/genética
Staphylococcus aureus Resistente à Meticilina/genética
Tipagem Molecular
Infecções Estafilocócicas/microbiologia
Zâmbia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Exotoxins); 0 (Leukocidins); 0 (Panton-Valentine leukocidin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.26.108.10982



página 1 de 133 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde