Base de dados : MEDLINE
Pesquisa : D12.776.543.750.655 [Categoria DeCS]
Referências encontradas : 58 [refinar]
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[PMID]:28492503
[Au] Autor:Pantho AF; Price M; Ashraf AZ; Wajid U; Khansari ME; Jahan A; Afroze SH; Rhaman MM; Johnson CR; Kuehl TJ; Hossain MA; Uddin MN
[Ad] Endereço:Department of Biochemistry, University of Texas at Austin, Austin, TX 78712, USA. ahmedfaiaz1995@utexas.edu.
[Ti] Título:Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells.
[So] Source:Int J Environ Res Public Health;14(5), 2017 May 11.
[Is] ISSN:1660-4601
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The cytotrophoblast (CTB) cells of the human placenta have membrane receptors that bind certain cardiotonic steroids (CTS) found in blood plasma. One of these, marinobufagenin, is a key factor in the etiology of preeclampsia. Herein, we used synthetic receptors (SR) to study their effectiveness on the angiogenic profile of human first trimester CTB cells. The humanextravillous CTB cells (Sw.71) used in this study were derived from first trimester chorionic villus tissue. Culture media of CTB cells treated with ≥1 nM SR level revealed sFlt-1 (Soluble fms-like tyrosine kinase-1) was significantly increased while VEGF (vascular endothelial growth factor) was significantly decreased in the culture media (* < 0.05 for each) The AT2 receptor (Angiotensin II receptor type 2) expression was significantly upregulated in ≥1 nM SR-treated CTB cells as compared to basal; however, the AT1 (Angiotensin II receptor, type 1) and VEGFR-1 (vascular endothelial growth factor receptor 1) receptor expression was significantly downregulated (* < 0.05 for each). Our results show that the anti-proliferative and anti-angiogenic effects of SR on CTB cells are similar to the effects of CTS. The observed anti angiogenic activity of SR on CTB cells demonstrates that the functionalized-urea/thiourea molecules may be useful as potent inhibitors to prevent CTS-induced impairment of CTB cells.
[Mh] Termos MeSH primário: Receptores Artificiais/metabolismo
Trofoblastos/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular
Feminino
Seres Humanos
Neovascularização Fisiológica
Gravidez
Primeiro Trimestre da Gravidez
Transdução de Sinais
Estresse Fisiológico
Fator A de Crescimento do Endotélio Vascular/metabolismo
Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Artificial); 0 (VEGFA protein, human); 0 (Vascular Endothelial Growth Factor A); EC 2.7.10.1 (FLT1 protein, human); EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


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[PMID]:28381789
[Au] Autor:Arimura T; Do JH; Tanaka F
[Ad] Endereço:National Institute of Advanced Industrial Science and Technology (AIST).
[Ti] Título:Electrochemically Switchable Molecular-Tweezers.
[So] Source:J Oleo Sci;66(4):419-423, 2017.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Synthetic receptors possessing two complexing chromophores connected by a single spacer are referred to as molecular tweezers. We report an electrochemically triggered molecular tweezers, which is a calix[4]arene-bis-porphyrin conjugate, that acts as a proof-of-concept demonstration system showing an electro-statically induced approach to guest release. The electrochemical behavior represents that 1,4-diazabicyclo[2.2.2]octane (DABCO) is released from the complex formed between calix[4]arene-bis-porphyrin conjugate and DABCO, just after cooperative two-oxidation occurs at 0.41 V.
[Mh] Termos MeSH primário: Calixarenos/química
Substâncias Macromoleculares/química
Porfirinas/química
Receptores Artificiais/química
[Mh] Termos MeSH secundário: Técnicas Eletroquímicas
Estrutura Molecular
Oxirredução
Piperazinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Macromolecular Substances); 0 (Piperazines); 0 (Porphyrins); 0 (Receptors, Artificial); 130036-26-9 (Calixarenes); X8M57R0JS5 (triethylenediamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17027


  3 / 58 MEDLINE  
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[PMID]:28283031
[Au] Autor:Patel C; Loughran D; Jones R; Abdulmajed M; Shergill I
[Ad] Endereço:Department of Urology, Wrexham Maelor Hospital, Croesnewydd Rd, Wrexham, LL13 7TD, Wales. chigsypatel@doctors.org.uk.
[Ti] Título:The resonance® metallic ureteric stent in the treatment of chronic ureteric obstruction: a safety and efficacy analysis from a contemporary clinical series.
[So] Source:BMC Urol;17(1):16, 2017 Mar 10.
[Is] ISSN:1471-2490
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We evaluate the efficacy and safety of metallic ureteric stenting using the Cook Resonance® stent in the treatment of chronic ureteric obstruction of benign and malignant aetiology. Published experience of using this stent in this context is limited. We add to the body of literature on this topic. METHODS: All patients who had a Resonance® metallic stent inserted between April 2009 and November 2014 in our institution were identified from a prospectively maintained stent-database. Primary outcome was relief of ureteric obstruction, defined by successful clinical and radiological treatment of hydronephrosis/hydroureter. Secondary outcome measures included operative time, radiological exposure, total stent dwell time (defined as the cumulative time in months for which a Resonance® metallic stent was in situ), and early and late complications. RESULTS: Twenty-one patients underwent 52 stent insertion episodes (SIE). Median age was 58 years (range 39-90). Stent insertion resulted in successful treatment of hydronephrosis/hydroureter in 96% (2 SIE resulted in failure to relieve ureteric obstruction). Median operative time was 21 min (range 12-90) Median radiation exposure was 815.3 cGy/cm2 (range 192.9-5366.3). Median stent dwell time was 19.5 months (range 6-52) in non-malignant and 12 months (range 2-48) in malignant ureteric obstruction. One stent migrated proximally during insertion and had to be retrieved using an antegrade approach. 5 patients re-admitted with haematuria: all resolved without intervention or blood transfusion. 3 episodes of post-operative urinary infection were recorded; all were successfully treated with oral antibiotics. CONCLUSION: Metallic ureteric stenting using the Resonance® stent is safe and effective for treating ureteric obstruction from both malignant and benign causes. The success rate in our series is 96%.
[Mh] Termos MeSH primário: Implante de Prótese/instrumentação
Stents
Obstrução Ureteral/cirurgia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Doença Crônica
Feminino
Seres Humanos
Masculino
Metais
Meia-Idade
Receptores Artificiais
Resultado do Tratamento
Ureter
Obstrução Ureteral/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals); 0 (Receptors, Artificial)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE
[do] DOI:10.1186/s12894-017-0204-8


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[PMID]:28052667
[Au] Autor:Hatanaka W; Kawaguchi M; Sun X; Nagao Y; Ohshima H; Hashida M; Higuchi Y; Kishimura A; Katayama Y; Mori T
[Ti] Título:Use of Membrane Potential to Achieve Transmembrane Modification with an Artificial Receptor.
[So] Source:Bioconjug Chem;28(2):296-301, 2017 Feb 15.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that was connected to biotin through a transmembrane oligoethylene glycol (OEG) chain. This artificial receptor gradually disappeared from the cell membrane via penetration despite the presence of a hydrophilic OEG chain. However, when the receptor was bound to streptavidin (SA), it remained on the cell membrane because of the large and hydrophilic nature of SA.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Potenciais da Membrana
Receptores Artificiais/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Espaço Intracelular/metabolismo
Células K562
Polietilenoglicóis/química
Receptores Artificiais/química
Solubilidade
Estreptavidina/metabolismo
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Artificial); 059QF0KO0R (Water); 30IQX730WE (Polyethylene Glycols); 9013-20-1 (Streptavidin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.6b00449


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[PMID]:27693353
[Au] Autor:Roybal KT; Williams JZ; Morsut L; Rupp LJ; Kolinko I; Choe JH; Walker WJ; McNally KA; Lim WA
[Ad] Endereço:Department of Cellular & Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158, USA; UCSF Center for Systems and Synthetic Biology, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, San Francisco, CA 94158, USA.
[Ti] Título:Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
[So] Source:Cell;167(2):419-432.e16, 2016 Oct 06.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Redirecting T cells to attack cancer using engineered chimeric receptors provides powerful new therapeutic capabilities. However, the effectiveness of therapeutic T cells is constrained by the endogenous T cell response: certain facets of natural response programs can be toxic, whereas other responses, such as the ability to overcome tumor immunosuppression, are absent. Thus, the efficacy and safety of therapeutic cells could be improved if we could custom sculpt immune cell responses. Synthetic Notch (synNotch) receptors induce transcriptional activation in response to recognition of user-specified antigens. We show that synNotch receptors can be used to sculpt custom response programs in primary T cells: they can drive a la carte cytokine secretion profiles, biased T cell differentiation, and local delivery of non-native therapeutic payloads, such as antibodies, in response to antigen. SynNotch T cells can thus be used as a general platform to recognize and remodel local microenvironments associated with diverse diseases.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Engenharia Celular
Neoplasias/terapia
Receptores Artificiais/imunologia
Receptores Notch/imunologia
[Mh] Termos MeSH secundário: Anticorpos/imunologia
Linhagem Celular Tumoral
Citocinas/imunologia
Citotoxicidade Imunológica
Seres Humanos
Imunoterapia/métodos
Ativação Linfocitária
Receptores Artificiais/genética
Receptores Notch/genética
Ligante Indutor de Apoptose Relacionado a TNF/imunologia
Células Th1/imunologia
Transcrição Genética
Microambiente Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies); 0 (Cytokines); 0 (Receptors, Artificial); 0 (Receptors, Notch); 0 (TNF-Related Apoptosis-Inducing Ligand)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


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[PMID]:27314397
[Au] Autor:Hammond GD; Vojta AL; Grant SA; Hunt HK
[Ad] Endereço:Department of Bioengineering, University of Missouri, Columbia, MO 65211, USA. gdh52b@mail.missouri.edu.
[Ti] Título:Integrating Nanostructured Artificial Receptors with Whispering Gallery Mode Optical Microresonators via Inorganic Molecular Imprinting Techniques.
[So] Source:Biosensors (Basel);6(2):26, 2016 Jun 15.
[Is] ISSN:2079-6374
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The creation of label-free biosensors capable of accurately detecting trace contaminants, particularly small organic molecules, is of significant interest for applications in environmental monitoring. This is achieved by pairing a high-sensitivity signal transducer with a biorecognition element that imparts selectivity towards the compound of interest. However, many environmental pollutants do not have corresponding biorecognition elements. Fortunately, biomimetic chemistries, such as molecular imprinting, allow for the design of artificial receptors with very high selectivity for the target. Here, we perform a proof-of-concept study to show how artificial receptors may be created from inorganic silanes using the molecular imprinting technique and paired with high-sensitivity transducers without loss of device performance. Silica microsphere Whispering Gallery Mode optical microresonators are coated with a silica thin film templated by a small fluorescent dye, fluorescein isothiocyanate, which serves as our model target. Oxygen plasma degradation and solvent extraction of the template are compared. Extracted optical devices are interacted with the template molecule to confirm successful sorption of the template. Surface characterization is accomplished via fluorescence and optical microscopy, ellipsometry, optical profilometry, and contact angle measurements. The quality factors of the devices are measured to evaluate the impact of the coating on device sensitivity. The resulting devices show uniform surface coating with no microstructural damage with Q factors above 106. This is the first report demonstrating the integration of these devices with molecular imprinting techniques, and could lead to new routes to biosensor creation for environmental monitoring.
[Mh] Termos MeSH primário: Técnicas Biossensoriais
Impressão Molecular
Nanoestruturas
Dispositivos Ópticos
Receptores Artificiais
[Mh] Termos MeSH secundário: Microesferas
Impressão Molecular/métodos
Dióxido de Silício/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Artificial); 7631-86-9 (Silicon Dioxide)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE


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[PMID]:27046098
[Au] Autor:Zhang YM; Zhang XJ; Xu X; Fu XN; Hou HB; Liu Y
[Ad] Endereço:Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University , Tianjin 300071, P. R. China.
[Ti] Título:Rigid Organization of Fluorescence-Active Ligands by Artificial Macrocyclic Receptor to Achieve the Thioflavin T-Amyloid Fibril Level Association.
[So] Source:J Phys Chem B;120(16):3932-40, 2016 04 28.
[Is] ISSN:1520-5207
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The push-pull molecules with an intramolecular charge transfer from donor to acceptor sides upon excitation exhibit a wide variety of biological and electronic activities, as exemplified by the in vivo fluorescence imaging probes for amyloid fibrils in the diagnosis and treatment of amyloid diseases. Interestingly, the structurally much simpler bis(4,8-disulfonato-1,5-naphtho)-32-crown-8 (DNC), in keen contrast to the conventional macrocyclic receptors, was found to dramatically enhance the fluorescence of twisted intramolecular charge-transfer molecules possessing various benzothiazolium and stilbazolium fluorophores upon complexation. Spectroscopic and microcalorimetric titrations jointly demonstrated the complex structures and the interactions that promote the extremely strong complexation, revealing that the binding affinity in these artificial host-guest pairs could reach up to a nearly 10(7) M(-1) order of magnitude in water, and the sandwich-type complexation is driven by electrostatic, hydrophobic, π-stacking, and hydrogen-bonding interactions. Quantum chemical calculations on free molecules and their DNC-bound species in both the ground and excited states elucidated that the encapsulation by DNC could greatly deter the central single and double chemical bonds from free intramolecular rotation in the singlet excited state, thus leading to the unique and unprecedented fluorescence enhancement upon sandwich-type complexation. This complexation-induced structural reorganization mechanism may also apply to the binding of other small-molecule ligands by functional receptors and contribute to the molecular-level understanding of the receptor-ligand interactions in many biology-related systems.
[Mh] Termos MeSH primário: Amiloide/química
Fluorescência
Receptores Artificiais/química
Tiazóis/química
[Mh] Termos MeSH secundário: Corantes Fluorescentes/química
Ligantes
Conformação Molecular
Teoria Quântica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amyloid); 0 (Fluorescent Dyes); 0 (Ligands); 0 (Receptors, Artificial); 0 (Thiazoles); 2390-54-7 (thioflavin T)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160406
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jpcb.6b02646


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[PMID]:27045359
[Au] Autor:Gao B; Tan LL; Song N; Li K; Yang YW
[Ad] Endereço:International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, P. R. China. ywyang@jlu.edu.cn.
[Ti] Título:A high-yield synthesis of [m]biphenyl-extended pillar[n]arenes for an efficient selective inclusion of toluene and m-xylene in the solid state.
[So] Source:Chem Commun (Camb);52(34):5804-7, 2016 Apr 30.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:[m]Bp-ExPn with a rigid and nanometer-sized cavity, as an extended version of pillar[n]arene by replacing 1,4-dimethoxybenzene monomers with biphenyl entities, was synthesized for the first time. Intriguingly, toluene and m-xylene can be stably included within the cavity of [2]Bp-ExP6, which endows these newly developed synthetic receptors with great potential in the purification of petrochemicals.
[Mh] Termos MeSH primário: Compostos de Bifenilo/química
Compostos Macrocíclicos/química
Receptores Artificiais/química
Tolueno/química
Xilenos/química
[Mh] Termos MeSH secundário: Compostos de Bifenilo/síntese química
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Compostos Macrocíclicos/síntese química
Estrutura Molecular
Espectroscopia de Prótons por Ressonância Magnética
Receptores Artificiais/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Macrocyclic Compounds); 0 (Receptors, Artificial); 0 (Xylenes); 3FPU23BG52 (Toluene); O9XS864HTE (3-xylene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160406
[St] Status:MEDLINE
[do] DOI:10.1039/c6cc01892k


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[PMID]:26822115
[Au] Autor:Rios P; Carter TS; Mooibroek TJ; Crump MP; Lisbjerg M; Pittelkow M; Supekar NT; Boons GJ; Davis AP
[Ad] Endereço:School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK.
[Ti] Título:Synthetic Receptors for the High-Affinity Recognition of O-GlcNAc Derivatives.
[So] Source:Angew Chem Int Ed Engl;55(10):3387-92, 2016 Mar 01.
[Is] ISSN:1521-3773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The combination of a pyrenyl tetraamine with an isophthaloyl spacer has led to two new water-soluble carbohydrate receptors ("synthetic lectins"). Both systems show outstanding affinities for derivatives of N-acetylglucosamine (GlcNAc) in aqueous solution. One receptor binds the methyl glycoside GlcNAc-ß-OMe with Ka ≈20,000 m(-1), whereas the other one binds an O-GlcNAcylated peptide with Ka ≈70,000 m(-1). These values substantially exceed those usually measured for GlcNAc-binding lectins. Slow exchange on the NMR timescale enabled structural determinations for several complexes. As expected, the carbohydrate units are sandwiched between the pyrenes, with the alkoxy and NHAc groups emerging at the sides. The high affinity of the GlcNAcyl-peptide complex can be explained by extra-cavity interactions, raising the possibility of a family of complementary receptors for O-GlcNAc in different contexts.
[Mh] Termos MeSH primário: Acetilglucosamina/metabolismo
Receptores Artificiais/metabolismo
[Mh] Termos MeSH secundário: Estrutura Molecular
Espectroscopia de Prótons por Ressonância Magnética
Receptores Artificiais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Receptors, Artificial); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160130
[St] Status:MEDLINE
[do] DOI:10.1002/anie.201510611


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[PMID]:26741147
[Au] Autor:Huang WC; Burnouf PA; Su YC; Chen BM; Chuang KH; Lee CW; Wei PK; Cheng TL; Roffler SR
[Ad] Endereço:Institute of Biomedical Science, Academia Sinica , Taipei 11529, Taiwan.
[Ti] Título:Engineering Chimeric Receptors To Investigate the Size- and Rigidity-Dependent Interaction of PEGylated Nanoparticles with Cells.
[So] Source:ACS Nano;10(1):648-62, 2016 Jan 26.
[Is] ISSN:1936-086X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Attachment of ligands to the surface of nanoparticles (NPs) is an attractive approach to target specific cells and increase intracellular delivery of nanocargos. To expedite investigation of targeted NPs, we engineered human cancer cells to express chimeric receptors that bind polyethylene glycol (PEG) and internalize stealth NPs in a fashion similar to ligand-targeted liposomes against epidermal growth factor receptor 1 or 2 (HER1 or HER2), which are validated targets for cancer therapy. Measurement of the rate of endocytosis and lysosomal accumulation of small (80-94 nm) or large (180-220 nm) flexible liposomes or more rigid lipid-coated mesoporous silica particles in human HT29 colon cancer and SKBR3 breast cancer cells that express chimeric receptors revealed that larger and more rigid NPs were internalized more slowly than smaller and more flexible NPs. An exception is when both the small and large liposomes underwent endocytosis via HER2. HER1 mediated faster and greater uptake of NPs into cells but retained NPs less well as compared to HER2. Lysosomal accumulation of NPs internalized via HER1 was unaffected by NP rigidity but was inversely related to NP size, whereas large rigid NPs internalized by HER2 displayed increased lysosomal accumulation. Our results provide insight into the effects of NP properties on receptor-mediated endocytosis and suggest that anti-PEG chimeric receptors may help accelerate investigation of targeted stealth NPs.
[Mh] Termos MeSH primário: Engenharia Celular
Nanopartículas/química
Polietilenoglicóis/química
Receptor do Fator de Crescimento Epidérmico/genética
Receptor ErbB-2/genética
Receptores Artificiais/genética
[Mh] Termos MeSH secundário: Caveolina 1/genética
Caveolina 1/metabolismo
Linhagem Celular Tumoral
Endocitose/efeitos dos fármacos
Expressão Gênica
Células HT29
Seres Humanos
Lipossomos/química
Lipossomos/farmacologia
Lisossomos/química
Lisossomos/metabolismo
Tamanho da Partícula
Plasmídeos/química
Plasmídeos/metabolismo
Polietilenoglicóis/metabolismo
Ligação Proteica
Receptor do Fator de Crescimento Epidérmico/metabolismo
Receptor ErbB-2/metabolismo
Receptores Artificiais/metabolismo
Transdução Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Caveolin 1); 0 (Liposomes); 0 (Receptors, Artificial); 30IQX730WE (Polyethylene Glycols); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (ERBB2 protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160108
[St] Status:MEDLINE
[do] DOI:10.1021/acsnano.5b05661



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