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[PMID]: 29254918 [Au] Autor: Tong B; Zhang L; Li GP [Ad] Endereço: State Key of Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Huhhot 010071, China. [Ti] Título: Progress in the molecular and genetic modification breeding of beef cattle in China. [So] Source: Yi Chuan;39(11):984-1015, 2017 Nov 20. [Is] ISSN: 0253-9772 [Cp] País de publicação: China [La] Idioma: eng [Ab] Resumo: The studies of beef cattle breeding in China have been greatly improved with the rapid development of the international beef cattle industrialization. The beef cattle breeding technologies have rapidly transformed from traditional breeding to molecular marker-assisted breeding, genomic selection and genetic modification breeding. Hundreds of candidate genes and molecular markers associated with growth, meat quality, reproduction performance and diseases resistance have been identified, and some of them have already been used in cattle breeding. Genes and molecular markers associated with growth and development are focused on the growth hormone, muscle regulatory factors, myostatin and insulin-like growth factors. Meat quality is mediated by fatty acid transport and deposition related signals, calpains and calpain system, muscle regulatory factors and muscle growth regulation pathways. Reproduction performance is regulated by GnRH-FSH-LH, growth differentiation factor 9, prolactin receptor and forkhead box protein O1. Disease resistance is modulated by the major histocompatibility complex gene family, toll-like receptors, mannose-binding lectin and interferon gene signals. In this review, we summarize the most recent progress in beef cattle breeding in marker-assisted selection, genome-wide selection and genetic modification breeding, aiming to provide a reference for further genetic breeding research of beef cattle in China. [Mh] Termos MeSH primário: Cruzamento Bovinos/genética Carne [Mh] Termos MeSH secundário: Animais Metilação de DNA Proteína Forkhead Box O1/genética Lectina de Ligação a Manose/genética Receptores do FSH/genética Seleção Genética [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Forkhead Box Protein O1); 0 (Mannose-Binding Lectin); 0 (Receptors, FSH) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180306 [Lr] Data última revisão: 180306 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171220 [St] Status: MEDLINE [do] DOI: 10.16288/j.yczz.17-181

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[PMID]: 29236987 [Au] Autor: Kumar TR [Ad] Endereço: Division of Reproductive Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado. [Ti] Título: Extragonadal Actions of FSH: A Critical Need for Novel Genetic Models. [So] Source: Endocrinology;159(1):2-8, 2018 01 01. [Is] ISSN: 1945-7170 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Follicle-stimulating hormone (FSH) is critical for ovarian folliculogenesis and essential for female fertility. FSH binds to FSH receptors (FSHRs) and regulates estrogen production in ovarian granulosa cells to orchestrate female reproductive physiology. Ovarian senescence that occurs as a function of aging results in loss of estrogen production, and this is believed to be the major reason for bone loss in postmenopausal women. Although conflicting, studies in rodents and humans during the last decade have provided genetic, pharmacological, and physiological evidence that elevated FSH levels that occur in the face of normal or declining estrogen levels directly regulate bone mass and adiposity. Recently, an efficacious blocking polyclonal FSHß antibody was developed that inhibited ovariectomy-induced bone loss and triggered white-to-brown fat conversion accompanied by mitochondrial biogenesis in mice. Moreover, additional nongonadal targets of FSH action have been identified, and these include the female reproductive tract (endometrium and myometrium), the placenta, hepatocytes, and blood vessels. In this mini-review, I summarize these studies in mice and humans and discuss critical gaps in our knowledge, yet unanswered questions, and the rationale for developing novel genetic models to unambiguously address the extragonadal actions of FSH. [Mh] Termos MeSH primário: Envelhecimento Hormônio Foliculoestimulante/fisiologia Modelos Genéticos Receptores do FSH/agonistas Transdução de Sinais [Mh] Termos MeSH secundário: Adiposidade Animais Desenvolvimento Ósseo Feminino Hormônio Foliculoestimulante/genética Seres Humanos Fígado/fisiologia Masculino Camundongos Knockout Camundongos Transgênicos Placentação Gravidez Receptores do FSH/genética Receptores do FSH/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW [Nm] Nome de substância: 0 (Receptors, FSH); 9002-68-0 (Follicle Stimulating Hormone) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180221 [Lr] Data última revisão: 180221 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 171214 [St] Status: MEDLINE [do] DOI: 10.1210/en.2017-03118

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[PMID]: 29017919 [Au] Autor: Crepin R; Veggiani G; Djender S; Beugnet A; Planeix F; Pichon C; Moutel S; Amigorena S; Perez F; Ghinea N; de Marco A [Ad] Endereço: Tumor Target and Therapeutic Antibody - Identification Platform (TAb-IP), PSL Research University, Institut Curie, 26, Rue D'Ulm, Paris, France; CIC IGR Curie 1428, France. [Ti] Título: Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors. [So] Source: Biochem Biophys Res Commun;493(4):1567-1572, 2017 Dec 02. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class. [Mh] Termos MeSH primário: Biblioteca de Peptídeos Receptores do FSH/antagonistas & inibidores Receptores do FSH/imunologia Anticorpos de Domínio Único/genética Anticorpos de Domínio Único/imunologia [Mh] Termos MeSH secundário: Animais Especificidade de Anticorpos AMP Cíclico/metabolismo Feminino Hormônio Foliculoestimulante/farmacologia Células HEK293 Seres Humanos Imunização Células L (Linhagem Celular) Masculino Camundongos Domínios Proteicos Receptores do FSH/genética Proteínas Recombinantes/química Proteínas Recombinantes/genética Proteínas Recombinantes/imunologia Transdução de Sinais Solubilidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Peptide Library); 0 (Receptors, FSH); 0 (Recombinant Proteins); 0 (Single-Domain Antibodies); 9002-68-0 (Follicle Stimulating Hormone); E0399OZS9N (Cyclic AMP) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171030 [Lr] Data última revisão: 171030 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171012 [St] Status: MEDLINE

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[PMID]: 28942449 [Au] Autor: Wei S; Shen X; Gong Z; Deng Y; Lai L; Liang H [Ad] Endereço: College of Life Science and Engineering, Northwest Minzu University, Lanzhou, China. [Ti] Título: FSHR and LHR Expression and Signaling as Well as Maturation and Apoptosis of Cumulus-Oocyte Complexes Following Treatment with FSH Receptor Binding Inhibitor in Sheep. [So] Source: Cell Physiol Biochem;43(2):660-669, 2017. [Is] ISSN: 1421-9778 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: BACKGROUND/AIMS: Currently, it remains unknown whether FSH receptor binding inhibitor (FRBI) influences follicular development and reproduction functions in humans and animals. The present study aimed to investigate FRBI effects on in vitro maturation (IVM) and apoptosis of cumulus-oocyte complexes (COCs) of sheep, to determine the effect of FRBI on mRNA and protein levels of FSHR and LHR in COCs, and to elucidate the signal pathway of FRBI effects. METHODS: COCs were in vitro cultured for 24h in the IVM media supplemented with varying concentrations of FRBI (0, 10, 20, 30 and 40µg/mL) and FSH (10IU/mL). The harvested COCs were observed under an inverted microscope and maturation rates of COCs were determined. Real time RT-PCR and Western blotting were utilized to detect mRNA and protein levels of FSHR and LHR. The concentrations of FSH, LH and caspase-3 were determined using especial ELISA kits for sheep, respectively. RESULTS: Maturation rates of COCs decreased gradually as FRBI concentrations increased from 0 to 40µg/mL, reaching a bottom value of 23.76% of the FRBI-4 group. The maximal apoptosis rate was detected in the FRBI-4 group. IP3 contents of FRBI-3 and FRBI-4 groups were reduced as compared to control group (CG) and FSH groups (P<0.05). Levels of FSHR protein of FRBI-3 and FRBI-4 groups as well as LHR protein of FRBI-4 group were significantly less than that of CG and FSH group. FSH contents of four FRBI treatment groups were gradually decreased along with the supplementation doses of FRBI. Caspase-3 contents of FRBI groups were reduced with a maximum reduction of the FRBI-2 group. CONCLUSION: Our results revealed supplement of FRBI into IVM media could dose-dependently decrease the maturation rate and increase apoptosis rate of sheep COCs. A lower dose of FRBI treatment slightly promoted IP3 production, but a higher dose of FRBI reduced IP3 production. FRBI suppressed the mRNA and protein expression levels of FSHR and LHR in sheep COCs. Our study will help to therapy effectively ovarian diseases, improve ovarian and follicular functions, and further to promote fertility of humans and animals. [Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos Proteínas de Transporte/farmacologia Células do Cúmulo/efeitos dos fármacos Técnicas de Maturação in Vitro de Oócitos Oócitos/efeitos dos fármacos Fragmentos de Peptídeos/farmacologia Receptores do FSH/genética Receptores do LH/genética [Mh] Termos MeSH secundário: Animais Células Cultivadas Células do Cúmulo/citologia Feminino Regulação da Expressão Gênica/efeitos dos fármacos Técnicas de Maturação in Vitro de Oócitos/métodos Oócitos/citologia Oogênese/efeitos dos fármacos Ovinos Transdução de Sinais/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Carrier Proteins); 0 (Peptide Fragments); 0 (Receptors, FSH); 0 (Receptors, LH); 0 (alanyl-glutamyl-seryl-asparagyl-glutamyl-aspartyl-glycyl-tyrosine) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170925 [St] Status: MEDLINE [do] DOI: 10.1159/000480650

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[PMID]: 28831954 [Au] Autor: Xu H; Cao L; Wei Y; Zhang Y; Liang M [Ad] Endereço: 1Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,106 Nanjing Road,Qingdao 266071,People's Republic of China. [Ti] Título: Effects of different dietary DHA:EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis). [So] Source: Br J Nutr;118(3):179-188, 2017 Aug. [Is] ISSN: 1475-2662 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The present study was conducted to investigate the effects of dietary DHA and EPA on gonadal steroidogenesis in mature females and males, with a feeding trial on tongue sole, a typical marine teleost with sexual dimorphism. Three experimental diets differing basically in DHA:EPA ratio, that is, 0·68 (diet D:E-0·68), 1·09 (D:E-1·09) and 2·05 (D:E-2·05), were randomly assigned to nine tanks of 3-year-old tongue sole (ten females and fifteen males in each tank). The feeding trail lasted for 90 d before and during the spawning season. Fish were reared in a flowing seawater system and fed to apparent satiation twice daily. Compared with diet D:E-0·68, diet D:E-1·09 significantly enhanced the oestradiol production in females, whereas diet D:E-2·05 significantly enhanced the testosterone production in males. In ovaries, diet D:E-1·09 induced highest mRNA expression of follicle-stimulating hormone receptor (FSHR), steroidogenic acute regulatory protein, 17α-hydroxylase (P450c17) and 3ß-hydroxysteroid dehydrogenase (3ß-HSD). In testes, diet 2·05 resulted in highest mRNA expression of FSHR, cholesterol side-chain cleavage enzyme, P450c17 and 3ß-HSD. Fatty acid profiles in fish tissues reflected closely those of diets. Female fish had more gonadal EPA content but less DHA content than male fish, whereas there was a reverse observation in liver. In conclusion, the dietary DHA:EPA ratio, possibly combined with the dietary EPA:arachidonic acid ratio, differentially regulated sex steroid hormone synthesis in mature female and male tongue soles. Females seemed to require more EPA but less DHA for the gonadal steroidogenesis than males. The results are beneficial to sex-specific nutritive strategies in domestic teleost. [Mh] Termos MeSH primário: Dieta/veterinária Ácidos Docosa-Hexaenoicos/administração & dosagem Ácido Eicosapentaenoico/administração & dosagem Linguados/metabolismo Hormônios Esteroides Gonadais/biossíntese Gônadas/efeitos dos fármacos [Mh] Termos MeSH secundário: 17-Hidroxiesteroide Desidrogenases/genética 17-Hidroxiesteroide Desidrogenases/metabolismo Animais Ácido Araquidônico/administração & dosagem Ácido Araquidônico/análise Ácidos Docosa-Hexaenoicos/análise Ácido Eicosapentaenoico/análise Estradiol/biossíntese Estradiol/sangue Feminino Hormônios Esteroides Gonadais/sangue Gônadas/metabolismo Lipogênese/efeitos dos fármacos Masculino RNA Mensageiro/genética RNA Mensageiro/metabolismo Receptores do FSH/genética Receptores do FSH/metabolismo Esteroide 17-alfa-Hidroxilase/genética Esteroide 17-alfa-Hidroxilase/metabolismo Testosterona/biossíntese Testosterona/sangue [Pt] Tipo de publicação: JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL [Nm] Nome de substância: 0 (Gonadal Steroid Hormones); 0 (RNA, Messenger); 0 (Receptors, FSH); 25167-62-8 (Docosahexaenoic Acids); 27YG812J1I (Arachidonic Acid); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); AAN7QOV9EA (Eicosapentaenoic Acid); EC 1.1.- (17-Hydroxysteroid Dehydrogenases); EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase); EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170907 [Lr] Data última revisão: 170907 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170824 [St] Status: MEDLINE [do] DOI: 10.1017/S0007114517001891

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[PMID]: 28764642 [Au] Autor: Wu Q; Zhang J; Zhu P; Jiang W; Liu S; Ni M; Zhang M; Li W; Zhou Q; Cui Y; Xia X [Ad] Endereço: Institute of Laboratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002, People's Republic of China. [Ti] Título: The susceptibility of FSHB -211G > T and FSHR G-29A, 919A > G, 2039A > G polymorphisms to men infertility: an association study and meta-analysis. [So] Source: BMC Med Genet;18(1):81, 2017 Aug 01. [Is] ISSN: 1471-2350 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Male infertility is a complex disorder caused by genetic, developmental, endocrine, or environmental factors as well as unknown etiology. Polymorphisms in the follicle stimulating hormone beta subunit (FSHB) (rs10835638, c.-211G > T) and follicle stimulating hormone receptor (FSHR) (rs1394205, c.-29G > A; rs6165, c.919A > G; rs6166, c.2039 A > G) genes might disturb normal spermatogenesis and affect male reproductive ability. METHODS: To further ascertain the aforementioned effects, we conducted a case-control study of 255 infertile men and 340 fertile controls from South China using the Mass ARRAY method, which was analyzed by the t-tests and logistic regression analysis using SPSS for Windows 14.0. In addition, a meta-analysis was performed by combining our results with previous reports using STATA 12.0. RESULTS: In the FSHB or FSHR gene single nucleotide polymorphism (SNP) evaluation, no statistically-significant difference was found in the frequency of allelic variants or in genotype distribution between cases and controls. However, a significant association for the comparison of GAA (P: 0.022, OR: 0.63, 95%CI: 0.43-0.94) was seen between the oligozoospermia and controls in haplotype analysis of rs1394205/rs6165/rs6166. In the meta-analysis, rs6165G allele and rs6166 GG genotype were associated with increased risk of the male infertility. CONCLUSIONS: This study suggested that FSHR GAA haplotype would exert protective effects against male sterility, which indicated that the combination of three SNP genotypes of FSHR was predicted to have a much stronger impact than either one alone. Then in the meta-analysis, a significant association was seen between FSHR rs6165, rs6166 polymorphisms and male infertility. In terms of male infertility with multifactorial etiology, further studies with larger sample sizes and different ethnic backgrounds or other risk factors are warranted to clarify the potential role of FSHB and FSHR polymorphisms in the pathogenesis of male infertility. [Mh] Termos MeSH primário: Proteínas de Transporte/genética Glicopeptídeos/genética Infertilidade Masculina/genética Polimorfismo de Nucleotídeo Único Receptores do FSH/genética [Mh] Termos MeSH secundário: Adulto Grupo com Ancestrais do Continente Asiático/genética Estudos de Casos e Controles Predisposição Genética para Doença Seres Humanos Masculino [Pt] Tipo de publicação: JOURNAL ARTICLE; META-ANALYSIS [Nm] Nome de substância: 0 (Carrier Proteins); 0 (FSH-BI protein, human); 0 (Glycopeptides); 0 (Receptors, FSH) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170808 [Lr] Data última revisão: 170808 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170803 [St] Status: MEDLINE [do] DOI: 10.1186/s12881-017-0441-4

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[PMID]: 28611209 [Au] Autor: Xie Y; Chu L; Liu Y; Sham KWY; Li J; Cheng CHK [Ad] Endereço: School of Biomedical SciencesThe Chinese University of Hong Kong-Shandong University Joint Laboratory on Reproductive Genetics, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. [Ti] Título: The highly overlapping actions of Lh signaling and Fsh signaling on zebrafish spermatogenesis. [So] Source: J Endocrinol;234(3):233-246, 2017 Sep. [Is] ISSN: 1479-6805 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Gonadotropin signaling plays a pivotal role in the spermatogenesis of vertebrates, but exactly how gonadotropins regulate the process in non-mammalian species remains elusive. Using a gene knockout approach in zebrafish, we have previously demonstrated the non-canonical action of gonadotropin signaling on spermatogenesis by analyzing four single mutant lines ( , , and ) and three double mutant lines ( , and ). In this study, we further investigated the actions of gonadotropins on the testis by establishing three other double-mutant zebrafish lines ( , and ). All and mutant males were fertile. Analysis on the gonadosomatic index and testicular histology in these and mutants demonstrated that Lh signaling and Fsh signaling could functionally compensate each other in the testis. Intriguingly, it was found that the mutant male fish were also morphologically and histologically normal and functionally fertile, a phenomenon which could be explained by the cross-activation of Lhr by Fsh. We have demonstrated this cross-reactivity for the first time in zebrafish. Fsh was shown to activate Lhr using three different assay systems, in which Lh-Fshr activation was also confirmed. Taken together, we conclude that the action of Lh signaling and Fsh signaling is redundant in that either alone can support zebrafish spermatogenesis based on two observations. First, that either Lh signaling or Fsh signaling alone is sufficient to support male fertility. Second, that the two gonadotropin ligands could promiscuously activate both receptors. Apart from revealing the complexity of gonadotropin signaling in controlling male reproduction in zebrafish, this study also shed light toward a better understanding on the evolution of gonadotropin signaling in vertebrates from fish to mammals. [Mh] Termos MeSH primário: Receptores do FSH/metabolismo Receptores do LH/metabolismo Espermatogênese Testículo/metabolismo Proteínas de Peixe-Zebra/metabolismo Peixe-Zebra/metabolismo [Mh] Termos MeSH secundário: Animais Feminino Masculino Receptores do FSH/genética Receptores do LH/genética Transdução de Sinais Testículo/citologia Peixe-Zebra/genética Peixe-Zebra/crescimento & desenvolvimento Proteínas de Peixe-Zebra/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Receptors, FSH); 0 (Receptors, LH); 0 (Zebrafish Proteins) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171016 [Lr] Data última revisão: 171016 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170615 [St] Status: MEDLINE [do] DOI: 10.1530/JOE-17-0079

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[PMID]: 28538730 [Au] Autor: Liu P; Ji Y; Yuen T; Rendina-Ruedy E; DeMambro VE; Dhawan S; Abu-Amer W; Izadmehr S; Zhou B; Shin AC; Latif R; Thangeswaran P; Gupta A; Li J; Shnayder V; Robinson ST; Yu YE; Zhang X; Yang F; Lu P; Zhou Y; Zhu LL; Oberlin DJ; Davies TF; Reagan MR; Brown A; Kumar TR; Epstein S; Iqbal J; Avadhani NG; New MI; Molina H; van Klinken JB; Guo EX; Buettner C; Haider S; Bian Z; Sun L; Rosen CJ; Zaidi M [Ad] Endereço: Department of Medicine, and Mount Sinai Bone Program, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA. [Ti] Título: Blocking FSH induces thermogenic adipose tissue and reduces body fat. [So] Source: Nature;546(7656):107-112, 2017 06 01. [Is] ISSN: 1476-4687 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Menopause is associated with bone loss and enhanced visceral adiposity. A polyclonal antibody that targets the ß-subunit of the pituitary hormone follicle-stimulating hormone (Fsh) increases bone mass in mice. Here, we report that this antibody sharply reduces adipose tissue in wild-type mice, phenocopying genetic haploinsufficiency for the Fsh receptor gene Fshr. The antibody also causes profound beiging, increases cellular mitochondrial density, activates brown adipose tissue and enhances thermogenesis. These actions result from the specific binding of the antibody to the ß-subunit of Fsh to block its action. Our studies uncover opportunities for simultaneously treating obesity and osteoporosis. [Mh] Termos MeSH primário: Tecido Adiposo/metabolismo Adiposidade Subunidade beta do Hormônio Folículoestimulante/antagonistas & inibidores Termogênese [Mh] Termos MeSH secundário: Adipócitos/efeitos dos fármacos Adipócitos/metabolismo Tecido Adiposo/efeitos dos fármacos Tecido Adiposo Bege/efeitos dos fármacos Tecido Adiposo Bege/metabolismo Tecido Adiposo Branco/efeitos dos fármacos Tecido Adiposo Branco/metabolismo Adiposidade/efeitos dos fármacos Animais Anticorpos/imunologia Anticorpos/farmacologia Dieta Hiperlipídica/efeitos adversos Feminino Subunidade beta do Hormônio Folículoestimulante/imunologia Haploinsuficiência Masculino Camundongos Mitocôndrias/efeitos dos fármacos Mitocôndrias/metabolismo Obesidade/tratamento farmacológico Obesidade/prevenção & controle Osteoporose/tratamento farmacológico Ovariectomia Consumo de Oxigênio/efeitos dos fármacos Receptores do FSH/antagonistas & inibidores Receptores do FSH/genética Receptores do FSH/metabolismo Termogênese/efeitos dos fármacos Proteína Desacopladora 1/biossíntese [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL [Nm] Nome de substância: 0 (Antibodies); 0 (Follicle Stimulating Hormone, beta Subunit); 0 (Receptors, FSH); 0 (Ucp1 protein, mouse); 0 (Uncoupling Protein 1) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171025 [Lr] Data última revisão: 171025 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170525 [St] Status: MEDLINE [do] DOI: 10.1038/nature22342

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[PMID]: 28446136 [Au] Autor: Hugon-Rodin J; Sonigo C; Gompel A; Dodé C; Grynberg M; Binart N; Beau I [Ad] Endereço: Gynecology Endocrinology Unit, Port-Royal Cochin Hospital, University Paris Descartes, Paris, France. [Ti] Título: First mutation in the FSHR cytoplasmic tail identified in a non-pregnant woman with spontaneous ovarian hyperstimulation syndrome. [So] Source: BMC Med Genet;18(1):44, 2017 Apr 26. [Is] ISSN: 1471-2350 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Spontaneous ovarian hyperstimulation syndrome (sOHSS) is a rare event occurring mostly during natural pregnancy. Among described etiologies, some activating mutations of FSH receptor (FSHR) have been identified. CASE PRESENTATION: We report hereby the case of a non-pregnant women with three episodes of sOHSS. Hormonal evaluation was normal and no pituitary adenoma was detected. However, genetic analysis identified a novel heterozygous FSHR mutation (c.1901 G > A). This R634H mutation is the first described in the cytoplasmic tail of the receptor. Functional analysis failed to reveal constitutive activity of the mutant but a decreased cAMP production in response to FSH. The weak activity of this mutant is correlated with a markedly reduced cell surface expression. CONCLUSION: Pathophysiology of non gestationnal sOHSS is still ill established. The molecular characterization of this new mutant indicates that it might not be at play. Therefore, further investigations are needed to improve knowledge of the molecular mechanism of this syndrome. [Mh] Termos MeSH primário: Citoplasma/metabolismo Mutação Síndrome de Hiperestimulação Ovariana/genética Receptores do FSH/genética [Mh] Termos MeSH secundário: Adulto Sequência de Aminoácidos Animais Feminino Seres Humanos Receptores do FSH/química Homologia de Sequência de Aminoácidos [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Receptors, FSH) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170620 [Lr] Data última revisão: 170620 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170428 [St] Status: MEDLINE [do] DOI: 10.1186/s12881-017-0407-6

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[PMID]: 28402061 [Au] Autor: Mehl NS; Khalid M; Srisuwatanasagul S; Swangchan-Uthai T; Sirivaidyapong S [Ad] Endereço: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. [Ti] Título: Comparison of the ovarian and uterine reproductive parameters, and the ovarian mRNA and protein expression of LHR and FSHR between the prepubertal and adult female cats. [So] Source: Reprod Domest Anim;52 Suppl 2:41-44, 2017 Apr. [Is] ISSN: 1439-0531 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: This study aimed to evaluate and compare the ovarian and uterine characteristics along with the ovarian mRNA and protein expression of LHR and FSHR between the pre-pubertal and adult female cats. The uterine horns and ovaries were collected from pre-pubertal and adult female cats at their follicular, luteal and interoestrous stages of the oestrous cycle (n = 6/group). Endometrial and myometrial thickness, uterine gland diameter, ovarian weight and type of follicles were analysed. The mRNA and protein expression of LHR and FSHR was analysed by IHC and qPCR, respectively. The ovarian weight of pre-pubertal cats was significantly lower than that of adult cats. No differences were recorded in the numbers of primordial and primary follicles between the study groups, while adult luteal cats had significantly lower numbers of antral follicles compared to pre-pubertal cats. No differences in the ovarian expression of FSHR mRNA, LHR protein or mRNA were found between the pre-pubertal and adult cats, but significantly lower FSHR protein expression was found in pre-pubertal cats compared to adult luteal cats. [Mh] Termos MeSH primário: Folículo Ovariano/fisiologia Receptores do FSH/fisiologia Receptores do LH/fisiologia Útero/fisiologia [Mh] Termos MeSH secundário: Animais Gatos Ciclo Estral/fisiologia Feminino Expressão Gênica [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Receptors, FSH); 0 (Receptors, LH) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170921 [Lr] Data última revisão: 170921 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170413 [St] Status: MEDLINE [do] DOI: 10.1111/rda.12926

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