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Pesquisa : D12.776.543.750.695.400 [Categoria DeCS]
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  1 / 2799 MEDLINE  
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[PMID]:28455422
[Au] Autor:Ball CB; Solem AC; Meganck RM; Laederach A; Ramos SBV
[Ad] Endereço:Biochemistry and Biophysics Department, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
[Ti] Título:Impact of RNA structure on ZFP36L2 interaction with luteinizing hormone receptor mRNA.
[So] Source:RNA;23(8):1209-1223, 2017 08.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ZFP36L2 (L2) destabilizes AU-rich element (ARE)-containing transcripts and has been implicated in female fertility. We have shown that only one of three putative AREs within the 3' UTR of murine luteinizing hormone receptor mRNA, ARE2197 (UAUUUAU), is capable of interacting with L2. To assess whether structural elements of ARE2197 could explain this unique binding ability, we performed whole-transcript SHAPE-MaP (selective 2' hydroxyl acylation by primer extension-mutational profiling) of the full-length mLHR mRNA. The data revealed that the functional ARE2197 is located in a hairpin loop structure and most nucleotides are highly reactive. In contrast, each of the nonbinding AREs, 2301 and 2444, contains only a pentamer AUUUA; and in ARE2301 much of the ARE sequence is poorly accessible. Because the functional mARE was also found to be conserved in humans at the sequence level (ARE 2223), we decided to investigate whether binding and structure are also preserved. Similar to mouse, only one ARE in hLHR mRNA is capable of binding to L2; and it is also located in a hairpin structure, based on our SHAPE-MaP data. To investigate the role of secondary structure in the binding, we mutated specific nucleotides in both functional AREs. Mutations in the flexible stem region proximal to the loop that enforce strong base-pairing, drastically reduced L2 binding affinity; this confirms that the structural context is critical for L2 recognition of hARE2223. Collectively, our results suggest that a combination of minimal ARE sequence, placement of the ARE in a hairpin loop, and stem flexibility mediate high-affinity L2 binding to hLHR mRNA.
[Mh] Termos MeSH primário: Elementos Ricos em Adenilato e Uridilato/genética
RNA Mensageiro/metabolismo
Receptores do LH/metabolismo
Tristetraprolina/metabolismo
[Mh] Termos MeSH secundário: Animais
Pareamento de Bases
Sequência de Bases
Seres Humanos
Camundongos
Mutação/genética
Conformação de Ácido Nucleico
RNA Mensageiro/química
RNA Mensageiro/genética
Receptores do LH/genética
Alinhamento de Sequência
Tristetraprolina/química
Tristetraprolina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Receptors, LH); 0 (Tristetraprolin); 0 (Zfp36 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171223
[Lr] Data última revisão:
171223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1261/rna.060467.116


  2 / 2799 MEDLINE  
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[PMID]:28942449
[Au] Autor:Wei S; Shen X; Gong Z; Deng Y; Lai L; Liang H
[Ad] Endereço:College of Life Science and Engineering, Northwest Minzu University, Lanzhou, China.
[Ti] Título:FSHR and LHR Expression and Signaling as Well as Maturation and Apoptosis of Cumulus-Oocyte Complexes Following Treatment with FSH Receptor Binding Inhibitor in Sheep.
[So] Source:Cell Physiol Biochem;43(2):660-669, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Currently, it remains unknown whether FSH receptor binding inhibitor (FRBI) influences follicular development and reproduction functions in humans and animals. The present study aimed to investigate FRBI effects on in vitro maturation (IVM) and apoptosis of cumulus-oocyte complexes (COCs) of sheep, to determine the effect of FRBI on mRNA and protein levels of FSHR and LHR in COCs, and to elucidate the signal pathway of FRBI effects. METHODS: COCs were in vitro cultured for 24h in the IVM media supplemented with varying concentrations of FRBI (0, 10, 20, 30 and 40µg/mL) and FSH (10IU/mL). The harvested COCs were observed under an inverted microscope and maturation rates of COCs were determined. Real time RT-PCR and Western blotting were utilized to detect mRNA and protein levels of FSHR and LHR. The concentrations of FSH, LH and caspase-3 were determined using especial ELISA kits for sheep, respectively. RESULTS: Maturation rates of COCs decreased gradually as FRBI concentrations increased from 0 to 40µg/mL, reaching a bottom value of 23.76% of the FRBI-4 group. The maximal apoptosis rate was detected in the FRBI-4 group. IP3 contents of FRBI-3 and FRBI-4 groups were reduced as compared to control group (CG) and FSH groups (P<0.05). Levels of FSHR protein of FRBI-3 and FRBI-4 groups as well as LHR protein of FRBI-4 group were significantly less than that of CG and FSH group. FSH contents of four FRBI treatment groups were gradually decreased along with the supplementation doses of FRBI. Caspase-3 contents of FRBI groups were reduced with a maximum reduction of the FRBI-2 group. CONCLUSION: Our results revealed supplement of FRBI into IVM media could dose-dependently decrease the maturation rate and increase apoptosis rate of sheep COCs. A lower dose of FRBI treatment slightly promoted IP3 production, but a higher dose of FRBI reduced IP3 production. FRBI suppressed the mRNA and protein expression levels of FSHR and LHR in sheep COCs. Our study will help to therapy effectively ovarian diseases, improve ovarian and follicular functions, and further to promote fertility of humans and animals.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Proteínas de Transporte/farmacologia
Células do Cúmulo/efeitos dos fármacos
Técnicas de Maturação in Vitro de Oócitos
Oócitos/efeitos dos fármacos
Fragmentos de Peptídeos/farmacologia
Receptores do FSH/genética
Receptores do LH/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Células do Cúmulo/citologia
Feminino
Regulação da Expressão Gênica/efeitos dos fármacos
Técnicas de Maturação in Vitro de Oócitos/métodos
Oócitos/citologia
Oogênese/efeitos dos fármacos
Ovinos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Peptide Fragments); 0 (Receptors, FSH); 0 (Receptors, LH); 0 (alanyl-glutamyl-seryl-asparagyl-glutamyl-aspartyl-glycyl-tyrosine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE
[do] DOI:10.1159/000480650


  3 / 2799 MEDLINE  
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[PMID]:28611209
[Au] Autor:Xie Y; Chu L; Liu Y; Sham KWY; Li J; Cheng CHK
[Ad] Endereço:School of Biomedical SciencesThe Chinese University of Hong Kong-Shandong University Joint Laboratory on Reproductive Genetics, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
[Ti] Título:The highly overlapping actions of Lh signaling and Fsh signaling on zebrafish spermatogenesis.
[So] Source:J Endocrinol;234(3):233-246, 2017 Sep.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gonadotropin signaling plays a pivotal role in the spermatogenesis of vertebrates, but exactly how gonadotropins regulate the process in non-mammalian species remains elusive. Using a gene knockout approach in zebrafish, we have previously demonstrated the non-canonical action of gonadotropin signaling on spermatogenesis by analyzing four single mutant lines ( , , and ) and three double mutant lines ( , and ). In this study, we further investigated the actions of gonadotropins on the testis by establishing three other double-mutant zebrafish lines ( , and ). All and mutant males were fertile. Analysis on the gonadosomatic index and testicular histology in these and mutants demonstrated that Lh signaling and Fsh signaling could functionally compensate each other in the testis. Intriguingly, it was found that the mutant male fish were also morphologically and histologically normal and functionally fertile, a phenomenon which could be explained by the cross-activation of Lhr by Fsh. We have demonstrated this cross-reactivity for the first time in zebrafish. Fsh was shown to activate Lhr using three different assay systems, in which Lh-Fshr activation was also confirmed. Taken together, we conclude that the action of Lh signaling and Fsh signaling is redundant in that either alone can support zebrafish spermatogenesis based on two observations. First, that either Lh signaling or Fsh signaling alone is sufficient to support male fertility. Second, that the two gonadotropin ligands could promiscuously activate both receptors. Apart from revealing the complexity of gonadotropin signaling in controlling male reproduction in zebrafish, this study also shed light toward a better understanding on the evolution of gonadotropin signaling in vertebrates from fish to mammals.
[Mh] Termos MeSH primário: Receptores do FSH/metabolismo
Receptores do LH/metabolismo
Espermatogênese
Testículo/metabolismo
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Receptores do FSH/genética
Receptores do LH/genética
Transdução de Sinais
Testículo/citologia
Peixe-Zebra/genética
Peixe-Zebra/crescimento & desenvolvimento
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, FSH); 0 (Receptors, LH); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-17-0079


  4 / 2799 MEDLINE  
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[PMID]:28605466
[Au] Autor:Gulappa T; Menon B; Menon KMJ
[Ad] Endereço:Department of Obstetrics/Gynecology, University of Michigan Medical School, Ann Arbor, Michigan 48109.
[Ti] Título:LHCGR Expression During Follicle Stimulating Hormone-Induced Follicle Growth Is Negatively Regulated by Eukaryotic Initiation Factor 5A.
[So] Source:Endocrinology;158(8):2672-2679, 2017 Aug 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have shown that the transient changes in the expression of luteinizing hormone/choriogonadotropin receptor (LHCGR) messenger RNA (mRNA) during the ovarian cycle occurs, at least in part, through a posttranscriptional mechanism involving an LHCGR mRNA-binding protein (LRBP). Eukaryotic initiation factor 5A (eIF5A), an LRBP-interacting protein, participates in this process. eIF5A undergoes hypusination, a unique posttranslational modification that is necessary for its functions. This study examined the role of eIF5A in follicle-stimulating hormone (FSH)-induced LHCGR expression during follicular growth. Treatment of primary cultures of rat granulosa cells with FSH and 17ß-estradiol (E2) showed a time-dependent increase in LHCGR mRNA expression. Conversely, inhibition of endogenous hypusination of eIF5A using N1-guanyl-1,7-diaminoheptane (GC7), a hypusination inhibitor, showed a greater increase in LHCGR mRNA expression over that produced by FSH and E2 alone. Further studies were carried out to determine the mechanism by which inhibition of hypusination of eIF5A causes an increase in LHCGR mRNA expression. Because LHCGR expression is negatively regulated by LRBP, the effect of inhibiting hypusination of eIF5A on LRBP expression was examined. The results showed a decrease in the expression of LRBP mRNA and protein when hypusination of eIF5A was inhibited by GC7. Because LRBP promotes LHCGR mRNA degradation, the results of this study support the notion that by inhibiting eIF5A hypusination, FSH reduces the expression of LRBP. This increases LHCGR mRNA expression by abrogating the inhibitory action of LRBP.
[Mh] Termos MeSH primário: Hormônio Foliculoestimulante/farmacologia
Fatores de Iniciação de Peptídeos/metabolismo
Proteínas de Ligação a RNA/metabolismo
Receptores do LH/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Regulação da Expressão Gênica/fisiologia
Células da Granulosa/fisiologia
Fatores de Iniciação de Peptídeos/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteínas de Ligação a RNA/genética
Ratos
Ratos Sprague-Dawley
Receptores do LH/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (LHCGR protein, rat); 0 (Peptide Initiation Factors); 0 (RNA, Messenger); 0 (RNA-Binding Proteins); 0 (Receptors, LH); 0 (eukaryotic translation initiation factor 5A); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00113


  5 / 2799 MEDLINE  
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[PMID]:28402061
[Au] Autor:Mehl NS; Khalid M; Srisuwatanasagul S; Swangchan-Uthai T; Sirivaidyapong S
[Ad] Endereço:Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
[Ti] Título:Comparison of the ovarian and uterine reproductive parameters, and the ovarian mRNA and protein expression of LHR and FSHR between the prepubertal and adult female cats.
[So] Source:Reprod Domest Anim;52 Suppl 2:41-44, 2017 Apr.
[Is] ISSN:1439-0531
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study aimed to evaluate and compare the ovarian and uterine characteristics along with the ovarian mRNA and protein expression of LHR and FSHR between the pre-pubertal and adult female cats. The uterine horns and ovaries were collected from pre-pubertal and adult female cats at their follicular, luteal and interoestrous stages of the oestrous cycle (n = 6/group). Endometrial and myometrial thickness, uterine gland diameter, ovarian weight and type of follicles were analysed. The mRNA and protein expression of LHR and FSHR was analysed by IHC and qPCR, respectively. The ovarian weight of pre-pubertal cats was significantly lower than that of adult cats. No differences were recorded in the numbers of primordial and primary follicles between the study groups, while adult luteal cats had significantly lower numbers of antral follicles compared to pre-pubertal cats. No differences in the ovarian expression of FSHR mRNA, LHR protein or mRNA were found between the pre-pubertal and adult cats, but significantly lower FSHR protein expression was found in pre-pubertal cats compared to adult luteal cats.
[Mh] Termos MeSH primário: Folículo Ovariano/fisiologia
Receptores do FSH/fisiologia
Receptores do LH/fisiologia
Útero/fisiologia
[Mh] Termos MeSH secundário: Animais
Gatos
Ciclo Estral/fisiologia
Feminino
Expressão Gênica
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, FSH); 0 (Receptors, LH)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.1111/rda.12926


  6 / 2799 MEDLINE  
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[PMID]:28360101
[Au] Autor:Matsuda M; Hirata M
[Ad] Endereço:From the Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan and natural@dent.kyushu-u.ac.jp.
[Ti] Título:Phospholipase C-related but catalytically inactive proteins regulate ovarian follicle development.
[So] Source:J Biol Chem;292(20):8369-8380, 2017 May 19.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phospholipase C-related but catalytically inactive proteins PRIP-1 and -2 are inositol-1,4,5-trisphosphate binding proteins that are encoded by independent genes. Ablation of the genes in mice impairs female fertility, which is manifested by fewer pregnancies, a decreased number of pups, and the decreased and increased secretion of gonadal steroids and gonadotropins, respectively. We investigated the involvement of the PRIPs in fertility, focusing on the ovaries of and double-knock-out (DKO) mice. Multiple cystic follicles were observed in DKO ovaries, and a superovulation assay showed a markedly decreased number of ovulated oocytes. Cumulus-oocyte complexes showed normal expansion, and artificial gonadotropin stimulation regulated the ovulation-related genes in a normal fashion, suggesting that the ovulation itself was probably normal. A histological analysis showed atresia in fewer follicles of the DKO ovaries, particularly in the secondary follicle stages. The expression of luteinizing hormone receptor (LHR) was aberrantly higher in developing follicles, and the phosphorylation of extracellular signal-regulated protein kinase, a downstream target of LH-LHR signaling, was higher in DKO granulosa cells. This suggests that the up-regulation of LH-LHR signaling is the cause of impaired follicle development. The serum estradiol level was lower, but estradiol production was unchanged in the DKO ovaries. These results suggest that PRIPs are positively involved in the development of follicles via their regulation of LH-LHR signaling and estradiol secretion. Female DKO mice had higher serum levels of insulin, testosterone, and uncarboxylated osteocalcin, which, together with reduced fertility, are reminiscent of polycystic ovary syndrome in humans.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
Folículo Ovariano/metabolismo
Receptores do LH/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Proteínas de Transporte/genética
Estradiol/genética
Estradiol/secreção
Feminino
Seres Humanos
Peptídeos e Proteínas de Sinalização Intracelular/genética
Hormônio Luteinizante/genética
Hormônio Luteinizante/metabolismo
Camundongos
Camundongos Knockout
Oócitos/metabolismo
Oócitos/patologia
Folículo Ovariano/patologia
Ovulação/genética
Síndrome do Ovário Policístico/genética
Síndrome do Ovário Policístico/metabolismo
Síndrome do Ovário Policístico/patologia
Receptores do LH/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Intracellular Signaling Peptides and Proteins); 0 (PRIP-1 phospholipase C-related protein, mouse); 0 (Plcl2 protein, mouse); 0 (Receptors, LH); 4TI98Z838E (Estradiol); 9002-67-9 (Luteinizing Hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.759928


  7 / 2799 MEDLINE  
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[PMID]:28337462
[Au] Autor:Ciesiólka S; Budna J; Jopek K; Bryja A; Kranc W; Borys S; Jeseta M; Chachula A; Ziólkowska A; Antosik P; Bukowska D; Brüssow KP; Bruska M; Nowicki M; Zabel M; Kempisty B
[Ad] Endereço:Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, Poland.
[Ti] Título:Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells.
[So] Source:Biomed Res Int;2017:9738640, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocyte maturation. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, and GCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term (168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6 M E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células da Granulosa/metabolismo
Oogênese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/genética
Família 19 do Citocromo P450/biossíntese
Estradiol/administração & dosagem
Feminino
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Células da Granulosa/efeitos dos fármacos
Seres Humanos
Técnicas de Maturação in Vitro de Oócitos
Oócitos/efeitos dos fármacos
Oócitos/crescimento & desenvolvimento
Oogênese/genética
Receptores do FSH/biossíntese
Receptores do FSH/genética
Receptores do LH/biossíntese
Receptores do LH/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, FSH); 0 (Receptors, LH); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Cytochrome P450 Family 19)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1155/2017/9738640


  8 / 2799 MEDLINE  
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[PMID]:28257870
[Au] Autor:De Los Reyes M; Palomino J; Parraguez VH; Ramirez F
[Ad] Endereço:Laboratory of Animal Reproduction, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile. Electronic address: mdlreyes@uchile.cl.
[Ti] Título:Analysis of LH receptor in canine ovarian follicles throughout the estrous cycle.
[So] Source:Theriogenology;93:71-77, 2017 Apr 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to determine the mRNA LHR and LHR protein expression pattern in the canine ovarian follicles at different stage of development throughout the estrous cycle. Dog ovaries were obtained from 1-6y bitches at proestrus/estrus, anestrus and diestrus stages following ovariohysterectomy. Follicular cells were mechanically recovered from follicles distributed into four types (preantral, small antral, medium antral and large antral). Total RNA extraction was performed and the evaluation of gene expression levels was achieved by relative quantification q-PCR analysis. Intrafollicular amounts of LHR were assessed by western blot method. All results were evaluated by ANOVA. The expression levels of mRNA LHR in follicular cells were observed in every stage of development, however this gene expression varied over the estrous cycle. LHR transcripts increased (P < 0.05) from preantral to antral stage. There were not differences in LHR gene expression among follicles at preantral stages; however, at antral stages the lowest (P < 0.05) LHR mRNA expression was found at anestrus and the highest (P < 0.05) at proestrus/estrus. The LHR protein was also detected in dog follicles in all reproductive phases with patterns varying with stage of follicular development over the reproductive cycle. The antibody against human LHR revealed two bands at ∼90 and ∼67 kDa, probably representing the matured protein and its precursor respectively. Both bands LHR appeared already at preantral follicles increasing (P < 0.05) with growth. A high proportion of LHR was presented as immature forms in all follicles stages during different phases of the estrous cycle. In conclusion, the gene and protein of LHR are differentially expressed in dog follicles over the estrous cycle, increasing with growth and the precursor protein is the most predominant LHR form present in canine follicles.
[Mh] Termos MeSH primário: Cães/metabolismo
Ciclo Estral/metabolismo
Folículo Ovariano/química
Receptores do LH/análise
Receptores do LH/genética
[Mh] Termos MeSH secundário: Anestro/metabolismo
Animais
Western Blotting/veterinária
Diestro/metabolismo
Estro/metabolismo
Feminino
Expressão Gênica
Histerectomia/veterinária
Ovariectomia/veterinária
Proestro/metabolismo
RNA Mensageiro/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Receptors, LH)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


  9 / 2799 MEDLINE  
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[PMID]:28228419
[Au] Autor:Zhang H; Zhang F; Zhu M; Wang J; Sheng X; Yuan Z; Han Y; Watanabe G; Taya K; Weng Q
[Ad] Endereço:Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; and.
[Ti] Título:Seasonal expressions of follicle-stimulating hormone receptor and luteinizing hormone receptor in the scented gland of the male muskrat ( ).
[So] Source:Am J Physiol Regul Integr Comp Physiol;312(4):R569-R574, 2017 Apr 01.
[Is] ISSN:1522-1490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence has shown that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may influence the functions of nongonadal tissues in addition to their classic target gonads. Our previous studies revealed that the scented glands of male muskrats expressed prolactin receptor, steroidogenic enzymes, and inhibin/activin subunits. To further seek the evidence of the activities of pituitary gonadotropins in scented glands, we investigated the seasonal expression patterns of FSH receptor (FSHR) and LH/choriogonadotropin receptor (LHCGR). The weight and size of scented glands during the breeding season were significantly higher than those during the nonbreeding season. Immunohistochemical studies showed that FSHR was present in the serous cells of scented glands, whereas LHCGR was present in the interstitial cells. The protein and mRNA expression levels of FSHR and LHCGR were significantly higher in the scented glands during the breeding season than those during the nonbreeding season. Importantly, the levels of circulating FSH and LH were remarkably higher during the breeding season. Taken together, these results suggested that gonadotropins may affect the function of muskrat scented gland via the locally expressed receptors in a season-dependent manner.
[Mh] Termos MeSH primário: Arvicolinae/fisiologia
Receptores do FSH/metabolismo
Receptores do LH/metabolismo
Glândulas Odoríferas/fisiologia
Estações do Ano
Comportamento Sexual Animal/fisiologia
[Mh] Termos MeSH secundário: Animais
Cruzamento
Regulação da Expressão Gênica/fisiologia
Masculino
Tamanho do Órgão/fisiologia
Especificidade de Órgãos
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, FSH); 0 (Receptors, LH)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE
[do] DOI:10.1152/ajpregu.00506.2016


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[PMID]:28188844
[Au] Autor:Regan SL; Knight PG; Yovich JL; Stanger JD; Leung Y; Arfuso F; Dharmarajan A; Almahbobi G
[Ad] Endereço:Stem Cell and Cancer Biology Laboratory, School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, Australia. Electronic address: sheenaregan@aapt.net.au.
[Ti] Título:Infertility and ovarian follicle reserve depletion are associated with dysregulation of the FSH and LH receptor density in human antral follicles.
[So] Source:Mol Cell Endocrinol;446:40-51, 2017 May 05.
[Is] ISSN:1872-8057
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The low take-home baby rate in older women in Australia (5.8%) undergoing IVF (5.8%) is linked to the depletion of the ovarian reserve of primordial follicles. Oocyte depletion causes an irreversible change to ovarian function. We found that the young patient FSH receptor and LH receptor expression profile on the granulosa cells collected from different size follicles were similar to the expression profile reported in natural cycles in women and sheep. This was reversed in the older patients with poor ovarian reserve. The strong correlation of BMPR1B and FSH receptor density in the young was not present in the older women; whereas, the LH receptor and BMPR1B correlation was weak in the young but was strongly correlated in the older women. The reduced fertilisation and pregnancy rate was associated with a lower LH receptor density and a lack of essential down-regulation of the FSH and LH receptor. The mechanism regulating FSH and LH receptor expression appears to function independently, in vivo, from the dose of FSH gonadotrophin, rather than in response to it. Restoring an optimum receptor density may improve oocyte quality and the pregnancy rate in older women.
[Mh] Termos MeSH primário: Infertilidade Feminina/metabolismo
Infertilidade Feminina/patologia
Folículo Ovariano/metabolismo
Reserva Ovariana
Receptores do FSH/metabolismo
Receptores do LH/metabolismo
[Mh] Termos MeSH secundário: Adulto
Animais
Receptores de Proteínas Morfogenéticas Ósseas Tipo I/metabolismo
Estrogênios/sangue
Feminino
Fertilização In Vitro
Células da Granulosa/metabolismo
Células da Granulosa/patologia
Seres Humanos
Infertilidade Feminina/sangue
Meia-Idade
Folículo Ovariano/crescimento & desenvolvimento
Progesterona/sangue
Reprodutibilidade dos Testes
Ovinos
Transdução de Sinais
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens); 0 (Receptors, FSH); 0 (Receptors, LH); 4G7DS2Q64Y (Progesterone); EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type I)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE



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