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[PMID]:28614384
[Au] Autor:Feng B; Guo Q; Zheng K; Qin Y; Du Y
[Ad] Endereço:Institute of Health and Environmental Ecology, Wenzhou Medical University, University Town, Wenzhou, Zhejiang, China.
[Ti] Título:Antennal transcriptome analysis of the piercing moth Oraesia emarginata (Lepidoptera: Noctuidae).
[So] Source:PLoS One;12(6):e0179433, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The piercing fruit moth Oraesia emarginata is an economically significant pest; however, our understanding of its olfactory mechanisms in infestation is limited. The present study conducted antennal transcriptome analysis of olfactory genes using real-time quantitative reverse transcription PCR analysis (RT-qPCR). We identified a total of 104 candidate chemosensory genes from several gene families, including 35 olfactory receptors (ORs), 41 odorant-binding proteins, 20 chemosensory proteins, 6 ionotropic receptors, and 2 sensory neuron membrane proteins. Seven candidate pheromone receptors (PRs) and 3 candidate pheromone-binding proteins (PBPs) for sex pheromone recognition were found. OemaOR29 and OemaPBP1 had the highest fragments per kb per million fragments (FPKM) values in all ORs and OBPs, respectively. Eighteen olfactory genes were upregulated in females, including 5 candidate PRs, and 20 olfactory genes were upregulated in males, including 2 candidate PRs (OemaOR29 and 4) and 2 PBPs (OemaPBP1 and 3). These genes may have roles in mediating sex-specific behaviors. Most candidate olfactory genes of sex pheromone recognition (except OemaOR29 and OemaPBP3) in O. emarginata were not clustered with those of studied noctuid species (type I pheromone). In addition, OemaOR29 was belonged to cluster PRIII, which comprise proteins that recognize type II pheromones instead of type I pheromones. The structure and function of olfactory genes that encode sex pheromones in O. emarginata might thus differ from those of other studied noctuids. The findings of the present study may help explain the molecular mechanism underlying olfaction and the evolution of olfactory genes encoding sex pheromones in O. emarginata.
[Mh] Termos MeSH primário: Antenas de Artrópodes/metabolismo
Perfilação da Expressão Gênica/métodos
Proteínas de Insetos/genética
Mariposas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Feminino
Ontologia Genética
Proteínas de Insetos/classificação
Masculino
Córtex Olfatório/metabolismo
Filogenia
Receptores Odorantes/classificação
Receptores Odorantes/genética
Receptores de Feromonas/classificação
Receptores de Feromonas/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência de Aminoácidos
Olfato/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Receptors, Odorant); 0 (Receptors, Pheromone); 0 (odorant-binding protein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179433


  2 / 288 MEDLINE  
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[PMID]:28423037
[Au] Autor:Wei HS; Li KB; Zhang S; Cao YZ; Yin J
[Ad] Endereço:State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus.
[So] Source:PLoS One;12(4):e0174036, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Loxostege sticticalis Linnaeus is an economically important agricultural pest, and the larvae cause great damage to crops, especially in Northern China. However, effective and environmentally friendly chemical methods for controlling this pest have not been discovered to date. In the present study, we performed HiSeq2500 sequencing of transcriptomes of the male and female adult antennae, adult legs and third instar larvae, and we identified 54 candidate odorant receptors (ORs), including 1 odorant receptor coreceptor (Orco) and 5 pheromone receptors (PRs), 18 ionotropic receptors (IRs), 13 gustatory receptors (GRs), 34 odorant binding proteins (OBPs), including 1 general odorant binding protein (GOBP1) and 3 pheromone binding proteins (PBPs), 10 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs). The results of RNA-Seq and RT-qPCR analyses showed the expression levels of most genes in the antennae were higher than that in the legs and larvae. Furthermore, PR4, OR1-4, 7-11, 13-15, 23, 29-32, 34, 41, 43, 47/IR7d.2/GR5b, 45, 7/PBP2-3, GOBP1, OBP3, 8 showed female antennae-biased expression, while PR1/OBP2, 7/IR75d/CSP2 showed male antennae-biased expression. However, IR1, 7d.3, 68a/OBP11, 20-22, 28/CSP9 had larvae enriched expression, and OBP15, 17, 25, 29/CSP5 were mainly expressed in the legs. The results shown above indicated that these genes might play a key role in foraging, seeking mates and host recognition in the L. sticticalis. Our findings will provide the basic knowledge for further studies on the molecular mechanisms of the olfactory system of L. sticticalis and potential novel targets for pest control strategies.
[Mh] Termos MeSH primário: Antenas de Artrópodes/metabolismo
Proteínas de Insetos/genética
Lepidópteros/genética
Receptores Ionotrópicos de Glutamato/genética
Receptores Odorantes/genética
Receptores de Feromonas/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Feminino
Perfilação da Expressão Gênica
Ontologia Genética
Proteínas de Insetos/metabolismo
Larva/genética
Lepidópteros/classificação
Masculino
Anotação de Sequência Molecular
Filogenia
Receptores Ionotrópicos de Glutamato/metabolismo
Receptores Odorantes/metabolismo
Receptores de Feromonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Receptors, Ionotropic Glutamate); 0 (Receptors, Odorant); 0 (Receptors, Pheromone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174036


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[PMID]:28390075
[Au] Autor:Feng B; Zheng K; Li C; Guo Q; Du Y
[Ad] Endereço:Institute of Health and Environmental Ecology, Wenzhou Medical University, University Town, Wenzhou, China.
[Ti] Título:A cytochrome P450 gene plays a role in the recognition of sex pheromones in the tobacco cutworm, Spodoptera litura.
[So] Source:Insect Mol Biol;26(4):369-382, 2017 Aug.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytochrome P450 (P450 or CYP) genes are involved in fundamental physiological functions, and might be also associated with the olfactory recognition of sex pheromones in beetles and moths. A P450 gene, Spodoptera litura CYP4L4 (SlituCYP4L4), was cloned for the first time from the antennae of S. litura. SlituCYP4L4 was almost exclusively expressed in the adult stage and predominantly expressed in the adult antennae. In situ hybridization showed that SlituCYP4L4 localized mainly at the base of the long sensilla trichoidea, which responds to sex pheromone components. Pretreatment with an S. litura sex pheromone significantly reduced the expression levels of SlituCYP4L4, consistent with other genes involved in sex pheromone recognition. The expression level of SlituCYP4L4 was different in moths collected with different ratios of sex pheromone lures and collected in different geographical locations. After gene knockdown of SlituCYP4L4 in the antennae, the electroantennogram (EAG) responses of male and female moths to (9Z,11E)-tetradecadienyl acetate or (9Z,12E)-tetradecadienyl acetate were significantly decreased. In contrast, EAG responses to plant volatiles and sex pheromones of other moth species were not significantly influenced in these moths. SlituCYP4L4 was also expressed in the gustatory tissues and sensilla, which suggests that SlituCYP4L4 may have other functions in the chemosensory system. Our results have shown for the first time the function of a CYP gene with appendage-specific expression in insect sex pheromone recognition, especially in adult moths.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Sensilas/metabolismo
Atrativos Sexuais/fisiologia
Spodoptera/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Transporte/metabolismo
Sistema Enzimático do Citocromo P-450/genética
Feminino
Proteínas de Insetos/metabolismo
Masculino
Dados de Sequência Molecular
Interferência de RNA
Receptores de Feromonas/metabolismo
Análise de Sequência de DNA
Spodoptera/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Insect Proteins); 0 (Receptors, Pheromone); 0 (Sex Attractants); 0 (cytochrome P-450 CYP4L4 (cabbage armyworm)); 0 (pheromone binding protein, insect); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12307


  4 / 288 MEDLINE  
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[PMID]:28237997
[Au] Autor:Yun YH; Oh MH; Kim JY; Kim SH
[Ad] Endereço:Department of Microbiology, Dankook University, Cheonan 31116, Republic of Korea.
[Ti] Título:, a Hybrid Histidine Kinase Gene, Is Essential for the Sexual Development and Virulence of .
[So] Source:J Microbiol Biotechnol;27(5):1010-1022, 2017 May 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Hybrid histidine kinase is part of a two-component system that is required for various stress responses and pathogenesis of pathogenic fungi. The gene in human pathogen encodes a hybrid histidine kinase and is important for pathogenesis. In this study, we identified a homolog, , in the maize pathogen by bioinformatics analysis. To explore the role of in the survival of under environmental stresses and its pathogenesis, mutants were constructed by allelic exchange. The growth of mutants was significantly impaired when they were cultured under hyperosmotic stress. The mutants exhibited increased resistance to antifungal agent fludioxonil. In particular, the mutants were unable to produce cytokinesis or conjugation tubes, and to develop fuzzy filaments, resulting in impaired mating between compatible strains. The expression levels of , and , which are involved in the pheromone pathway, were significantly decreased in the mutants. In inoculation tests to the host plant, the mutants showed significantly reduced ability in the production of anthocyanin pigments and tumor development on maize leaves. Overall, the combined results indicated that plays important roles in the survival under hyperosmotic stress, and contributes to cytokinesis, sexual development, and virulence of by regulating the expression of the genes involved in the pheromone pathway.
[Mh] Termos MeSH primário: Genes Fúngicos Tipo Acasalamento/genética
Histidina Quinase/genética
Desenvolvimento Sexual/genética
Ustilago/crescimento & desenvolvimento
Ustilago/patogenicidade
Virulência/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antocianinas/metabolismo
Antifúngicos/farmacologia
Cultura Axênica
Biologia Computacional
Citocinese
DNA Fúngico/genética
Dioxóis/farmacologia
Escherichia coli/genética
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Proteínas de Grupo de Alta Mobilidade/metabolismo
Histidina Quinase/classificação
Hiperostose
Mutação
Pressão Osmótica
Fenótipo
Feromônios/metabolismo
Filogenia
Doenças das Plantas/microbiologia
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Pirróis/farmacologia
RNA Mensageiro/análise
Receptores de Feromonas/metabolismo
Alinhamento de Sequência
Estresse Fisiológico/genética
Fatores de Transcrição/metabolismo
Ustilago/efeitos dos fármacos
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antifungal Agents); 0 (DNA, Fungal); 0 (Dioxoles); 0 (Fungal Proteins); 0 (High Mobility Group Proteins); 0 (Pheromones); 0 (Plant Proteins); 0 (Pyrroles); 0 (RNA, Messenger); 0 (Receptors, Pheromone); 0 (Transcription Factors); 0 (pheromone response factor 1, Ustilago maydis); EC 2.7.13.1 (Histidine Kinase); ENS9J0YM16 (fludioxonil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1702.02001


  5 / 288 MEDLINE  
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[PMID]:28207738
[Au] Autor:Lakhani V; Elston TC
[Ad] Endereço:Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
[Ti] Título:Testing the limits of gradient sensing.
[So] Source:PLoS Comput Biol;13(2):e1005386, 2017 Feb.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to detect a chemical gradient is fundamental to many cellular processes. In multicellular organisms gradient sensing plays an important role in many physiological processes such as wound healing and development. Unicellular organisms use gradient sensing to move (chemotaxis) or grow (chemotropism) towards a favorable environment. Some cells are capable of detecting extremely shallow gradients, even in the presence of significant molecular-level noise. For example, yeast have been reported to detect pheromone gradients as shallow as 0.1 nM/µm. Noise reduction mechanisms, such as time-averaging and the internalization of pheromone molecules, have been proposed to explain how yeast cells filter fluctuations and detect shallow gradients. Here, we use a Particle-Based Reaction-Diffusion model of ligand-receptor dynamics to test the effectiveness of these mechanisms and to determine the limits of gradient sensing. In particular, we develop novel simulation methods for establishing chemical gradients that not only allow us to study gradient sensing under steady-state conditions, but also take into account transient effects as the gradient forms. Based on reported measurements of reaction rates, our results indicate neither time-averaging nor receptor endocytosis significantly improves the cell's accuracy in detecting gradients over time scales associated with the initiation of polarized growth. Additionally, our results demonstrate the physical barrier of the cell membrane sharpens chemical gradients across the cell. While our studies are motivated by the mating response of yeast, we believe our results and simulation methods will find applications in many different contexts.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Quimiotaxia/fisiologia
Modelos Biológicos
Feromônios/farmacocinética
Receptores de Feromonas/metabolismo
Saccharomyces cerevisiae/fisiologia
[Mh] Termos MeSH secundário: Membrana Celular/química
Quimiotaxia/efeitos dos fármacos
Simulação por Computador
Difusão
Modelos Químicos
Modelos Estatísticos
Feromônios/química
Receptores de Feromonas/química
Saccharomyces cerevisiae/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones); 0 (Receptors, Pheromone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005386


  6 / 288 MEDLINE  
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[PMID]:27993568
[Au] Autor:Stoneman MR; Paprocki JD; Biener G; Yokoi K; Shevade A; Kuchin S; Raicu V
[Ad] Endereço:Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA. Electronic address: stonema2@uwm.edu.
[Ti] Título:Quaternary structure of the yeast pheromone receptor Ste2 in living cells.
[So] Source:Biochim Biophys Acta;1859(9 Pt A):1456-1464, 2017 09.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Transmembrane proteins known as G protein-coupled receptors (GPCRs) have been shown to form functional homo- or hetero-oligomeric complexes, although agreement has been slow to emerge on whether homo-oligomerization plays functional roles. Here we introduce a platform to determine the identity and abundance of differing quaternary structures formed by GPCRs in living cells following changes in environmental conditions, such as changes in concentrations. The method capitalizes on the intrinsic capability of FRET spectrometry to extract oligomer geometrical information from distributions of FRET efficiencies (or FRET spectrograms) determined from pixel-level imaging of cells, combined with the ability of the statistical ensemble approaches to FRET to probe the proportion of different quaternary structures (such as dimers, rhombus or parallelogram shaped tetramers, etc.) from averages over entire cells. Our approach revealed that the yeast pheromone receptor Ste2 forms predominantly tetramers at average expression levels of 2 to 25 molecules per pixel (2.8·10 to 3.5·10 molecules/nm ), and a mixture of tetramers and octamers at expression levels of 25-100 molecules per pixel (3.5·10 to 1.4·10 molecules/nm ). Ste2 is a class D GPCR found in the yeast Saccharomyces cerevisiae of the mating type a, and binds the pheromone α-factor secreted by cells of the mating type α. Such investigations may inform development of antifungal therapies targeting oligomers of pheromone receptors. The proposed FRET imaging platform may be used to determine the quaternary structure sub-states and stoichiometry of any GPCR and, indeed, any membrane protein in living cells. This article is part of a Special Issue entitled: Interactions between membrane receptors in cellular membranes edited by Kalina Hristova.
[Mh] Termos MeSH primário: Receptores Acoplados a Proteínas-G/química
Receptores de Fator de Acasalamento/química
Receptores de Feromonas/química
Proteínas de Saccharomyces cerevisiae/química
[Mh] Termos MeSH secundário: Membrana Celular/química
Membrana Celular/metabolismo
Transferência Ressonante de Energia de Fluorescência
Feromônios/metabolismo
Multimerização Proteica
Estrutura Quaternária de Proteína
Receptores Acoplados a Proteínas-G/genética
Receptores Acoplados a Proteínas-G/metabolismo
Receptores de Fator de Acasalamento/genética
Receptores de Fator de Acasalamento/metabolismo
Receptores de Feromonas/genética
Receptores de Feromonas/metabolismo
Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Pheromones); 0 (Receptors, G-Protein-Coupled); 0 (Receptors, Mating Factor); 0 (Receptors, Pheromone); 0 (STE2 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE


  7 / 288 MEDLINE  
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[PMID]:26573759
[Au] Autor:Zhang QH; Wu ZN; Zhou JJ; Du YJ
[Ad] Endereço:College of Life Sciences, Sichuan University, Chengdu 610065, China.
[Ti] Título:Molecular and functional characterization of a candidate sex pheromone receptor OR1 in Spodoptera litura.
[So] Source:Insect Sci;24(4):543-558, 2017 Aug.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Olfaction is primarily mediated by highly specified olfactory receptors (ORs). Here, we cloned and identified an olfactory receptor, named SlituOR1 (Genbank no. JN835269), from Spodoptera litura and found evidence that it is a candidate pheromone receptor. It exhibited male-biased expression in the antennae, where it was localized at the base of sensilla trichoidea, the antennal sensilla mainly responsive to pheromones in moths. Conserved orthologues of this receptor, found among known pheromone receptors within the Lepidoptera, and SlituOR1 were placed among a clade of candidate pheromone receptors in a phylogeny tree of insect OR gene sequences. SlituOR1 showed differential expression in S. litura populations attracted to traps baited with different ratios of the two sex pheromone components (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). Knocking down of SlituOR1 by RNA interference reduced the electroantennogram (EAG) response to Z9E11-14:OAc, and this result is consistent with the field trapping experiment. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could provide some of the flexibility required to maintain the functionality of communication with females when a population is adapting to a new niche and reproductive isolation becomes an advantage.
[Mh] Termos MeSH primário: Sensilas/metabolismo
Atrativos Sexuais/farmacologia
Spodoptera/genética
Spodoptera/fisiologia
[Mh] Termos MeSH secundário: Animais
Antenas de Artrópodes/efeitos dos fármacos
Antenas de Artrópodes/metabolismo
Fenômenos Eletrofisiológicos
Feminino
Perfilação da Expressão Gênica
Proteínas de Insetos/genética
Masculino
Filogenia
Interferência de RNA
Receptores de Feromonas/genética
Sensilas/efeitos dos fármacos
Análise de Sequência de DNA
Atrativos Sexuais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Receptors, Pheromone); 0 (Sex Attractants)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151118
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12294


  8 / 288 MEDLINE  
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[PMID]:27798845
[Au] Autor:Dudin O; Merlini L; Martin SG
[Ad] Endereço:Department of Fundamental Microbiology, University of Lausanne, CH-1015 Lausanne, Switzerland.
[Ti] Título:Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.
[So] Source:Genes Dev;30(19):2226-2239, 2016 Oct 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell fusion is universal in eukaryotes for fertilization and development, but what signals this process is unknown. Here, we show in Schizosaccharomyces pombe that fusion does not require a dedicated signal but is triggered by spatial focalization of the same pheromone-GPCR (G-protein-coupled receptor)-MAPK signaling cascade that drives earlier mating events. Autocrine cells expressing the receptor for their own pheromone trigger fusion attempts independently of cell-cell contact by concentrating pheromone release at the fusion focus, a dynamic actin aster underlying the secretion of cell wall hydrolases. Pheromone receptor and MAPK cascade are similarly enriched at the fusion focus, concomitant with fusion commitment in wild-type mating pairs. This focalization promotes cell fusion by immobilizing the fusion focus, thus driving local cell wall dissolution. We propose that fusion commitment is imposed by a local increase in MAPK concentration at the fusion focus, driven by a positive feedback between fusion focus formation and focalization of pheromone release and perception.
[Mh] Termos MeSH primário: Sistema de Sinalização das MAP Quinases/fisiologia
Feromônios/metabolismo
Proteínas de Schizosaccharomyces pombe/metabolismo
Schizosaccharomyces/citologia
Schizosaccharomyces/fisiologia
[Mh] Termos MeSH secundário: Comunicação Autócrina/fisiologia
Receptores de Feromonas/genética
Receptores de Feromonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones); 0 (Receptors, Pheromone); 0 (Schizosaccharomyces pombe Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


  9 / 288 MEDLINE  
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[PMID]:27567717
[Au] Autor:Doughan B; Rollins JA
[Ad] Endereço:Department of Plant Pathology, University of Florida, Gainesville, FL 32611-0680, USA.
[Ti] Título:Characterization of MAT gene functions in the life cycle of Sclerotinia sclerotiorum reveals a lineage-specific MAT gene functioning in apothecium morphogenesis.
[So] Source:Fungal Biol;120(9):1105-17, 2016 Sep.
[Is] ISSN:1878-6146
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sclerotinia sclerotiorum (Lib.) de Bary is a phytopathogenic fungus that relies on the completion of the sexual cycle to initiate aerial infections. The sexual cycle produces apothecia required for inoculum dispersal. In this study, insight into the regulation of apothecial multicellular development was pursued through functional characterization of mating-type genes. These genes are hypothesized to encode master regulatory proteins required for aspects of sexual development ranging from fertilization through fertile fruiting body development. Experimentally, loss-of-function mutants were created for the conserved core mating-type genes (MAT1-1-1, and MAT1-2-1), and the lineage-specific genes found only in S. sclerotiorum and closely related fungi (MAT1-1-5, and MAT1-2-4). The MAT1-1-1, MAT1-1-5, and MAT1-2-1 mutants are able to form ascogonia but are blocked in all aspects of apothecium development. These mutants also exhibit defects in secondary sexual characters including lower numbers of spermatia. The MAT1-2-4 mutants are delayed in carpogenic germination accompanied with altered disc morphogenesis and ascospore production. They too produce lower numbers of spermatia. All four MAT gene mutants showed alterations in the expression of putative pheromone precursor (Ppg-1) and pheromone receptor (PreA, PreB) genes. Our findings support the involvement of MAT genes in sexual fertility, gene regulation, meiosis, and morphogenesis in S. sclerotiorum.
[Mh] Termos MeSH primário: Ascomicetos/crescimento & desenvolvimento
Ascomicetos/genética
Genes Fúngicos Tipo Acasalamento
[Mh] Termos MeSH secundário: Ascomicetos/citologia
Regulação Fúngica da Expressão Gênica
Técnicas de Inativação de Genes
Mutação
Feromônios/biossíntese
Receptores de Feromonas/biossíntese
Esporos Fúngicos/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones); 0 (Receptors, Pheromone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160829
[St] Status:MEDLINE


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[PMID]:27538507
[Au] Autor:Hu P; Tao J; Cui M; Gao C; Lu P; Luo Y
[Ad] Endereço:Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, No.35 Tsinghua East Road, Haidian District, Beijing, 100083, People's Republic of China.
[Ti] Título:Antennal transcriptome analysis and expression profiles of odorant binding proteins in Eogystia hippophaecolus (Lepidoptera: Cossidae).
[So] Source:BMC Genomics;17:651, 2016 08 18.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Eogystia hippophaecolus (Hua et al.) (Lepidoptera: Cossidae) is the major threat to seabuckthorn plantations in China. Specific and highly efficient artificial sex pheromone traps was developed and used to control it. However, the molecular basis for the pheromone recognition is not known. So we established the antennal transcriptome of E. hippophaecolus and characterized the expression profiles of odorant binding proteins. These results establish and improve the basis knowledge of the olfactory receptive system, furthermore provide a theoretical basis for the development of new pest control method. RESULTS: We identified 29 transcripts encoding putative odorant-binding proteins (OBPs), 18 putative chemosensory proteins (CSPs), 63 odorant receptors (ORs), 13 gustatory receptors (GRs), 12 ionotropic receptors (IRs), and two sensory neuron membrane proteins (SNMPs). Based on phylogenetic analysis, we found one Orco and three pheromone receptors of E. hippophaecolus and found that EhipGR13 detects sugar, EhipGR11 and EhipGR3 detect bitter. Nine OBPs expression profile indicated that most were the highest expression in antennae, consistent with functions of OBPs in binding and transporting odors during the antennal recognition process. OBP6 was external expressed in male genital-biased in, and this locus may be responsible for pheromone binding and recognition as well as mating. OBP1 was the highest and biased expressed in the foot and may function as identification of host plant volatiles. CONCLUSIONS: One hundred thirty-seven chemosensory proteins were identified and the accurate functions and groups of part proteins were obtained by phylogenetic analysis. The most OBPs were antenna-biased expressed, which are involved in antennal recognition. However, few OBP was detected biased expression in the foot and external genitalia, and these loci may function in pheromone recognition, mating, and the recognition of plant volatiles.
[Mh] Termos MeSH primário: Antenas de Artrópodes/metabolismo
Perfilação da Expressão Gênica/métodos
Lepidópteros/genética
Receptores Odorantes/genética
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica
Ontologia Genética
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Masculino
Filogenia
Receptores Odorantes/metabolismo
Receptores de Feromonas/genética
Receptores de Feromonas/metabolismo
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Receptors, Odorant); 0 (Receptors, Pheromone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160820
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-3008-4



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