Base de dados : MEDLINE
Pesquisa : D12.776.543.750.695.862.540 [Categoria DeCS]
Referências encontradas : 1092 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 110 ir para página                         

  1 / 1092 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28088386
[Au] Autor:Xiaopeng B; Tanaka Y; Ihara E; Hirano K; Nakano K; Hirano M; Oda Y; Nakamura K
[Ad] Endereço:Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
[Ti] Título:Trypsin induces biphasic muscle contraction and relaxation via transient receptor potential vanilloid 1 and neurokinin receptors 1/2 in porcine esophageal body.
[So] Source:Eur J Pharmacol;797:65-74, 2017 Feb 15.
[Is] ISSN:1879-0712
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Duodenal reflux of fluids containing trypsin relates to refractory gastroesophageal reflux disease (GERD). Esophageal peristalsis and clearance are important factors in GERD pathogenesis. However, the function of trypsin in esophageal body contractility is not fully understood. In this study, effects of trypsin on circular smooth muscle (CSM) and longitudinal smooth muscle (LSM) of the porcine esophageal body were examined. Trypsin elicited a concentration dependent biphasic response, a major contraction and a subsequent relaxation only in CSM. In CSM, contraction occurred at trypsin concentrations of 100nM and relaxation at 1µM. A proteinase-activated receptor (PAR)2 activating peptide, SLIGKV-NH (1mM), induced a monophasic contraction. Those responses were unaffected by tetrodotoxin though abolished by the gap junction uncouplers carbenoxolone and octanol. They were also partially inhibited by a transient receptor potential vanilloid type 1 (TRPV1) antagonist and abolished by combination of neurokinin receptor 1 (NK ) and NK antagonists, but not by an NK antagonist, suggesting a PAR2-TRPV1-substance P pathway in sensory neurons. Substance P (100nM), an agonist for various NK receptors (NK , NK and NK ) with differing affinities, induced significant contraction in CSM, but not in LSM. The contraction was also blocked by the combination of NK and NK antagonists, but not by the NK antagonist. Moreover, substance P-induced contractions were unaffected by the TRPV1 antagonist, but inhibited by a gap junction uncoupler. In conclusion, trypsin induced a biphasic response only in CSM and this was mediated by PAR2, TRPV1 and NK . Gap junctions were indispensable in this tachykinin-induced response.
[Mh] Termos MeSH primário: Esôfago/efeitos dos fármacos
Contração Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Receptores da Neurocinina-1/metabolismo
Receptores da Neurocinina-2/metabolismo
Canais de Cátion TRPV/metabolismo
Tripsina/farmacologia
[Mh] Termos MeSH secundário: Animais
Epoprostenol/metabolismo
Esôfago/metabolismo
Esôfago/fisiologia
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Músculo Liso/fisiologia
Óxido Nítrico/metabolismo
Suínos
Quinases Associadas a rho/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Neurokinin-1); 0 (Receptors, Neurokinin-2); 0 (TRPV Cation Channels); 0 (TRPV1 protein, mouse); 31C4KY9ESH (Nitric Oxide); DCR9Z582X0 (Epoprostenol); EC 2.7.11.1 (rho-Associated Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3); EC 3.4.21.4 (Trypsin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170116
[St] Status:MEDLINE


  2 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27889808
[Au] Autor:Kullmann FA; Katofiasc M; Thor KB; Marson L
[Ad] Endereço:Department of Medicine, Renal Division, University of Pittsburgh, 3500 Terrace St, Scaife A1220, Pittsburgh, PA, 15261, USA.
[Ti] Título:Pharmacodynamic evaluation of Lys , MeLeu , Nle -NKA prokinetic effects on bladder and colon activity in acute spinal cord transected and spinally intact rats.
[So] Source:Naunyn Schmiedebergs Arch Pharmacol;390(2):163-173, 2017 Feb.
[Is] ISSN:1432-1912
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to determine feasibility of a novel therapeutic approach to drug-induced voiding after spinal cord injury (SCI) using a well-characterized, peptide, neurokinin 2 receptor (NK receptor) agonist, Lys , MeLeu , Nle -NKA (LMN-NKA). Cystometry and colorectal pressure measurements were performed in urethane-anesthetized, intact, and acutely spinalized female rats. Bladder pressure and voiding were monitored in response to intravenous LMN-NKA given with the bladder filled to 70% capacity. LMN-NKA (0.1-300 µg/kg) produced dose-dependent, rapid (<60 s), short-duration (<15 min) increases in bladder pressure. In intact rats, doses above 0.3-1 µg/kg induced urine release (voiding efficiency of ~70% at ≥1 µg/kg). In spinalized rats, urine release required higher doses (≥10 µg/kg) and was less efficient (30-50%). LMN-NKA (0.1-100 µg/kg) also produced dose-dependent increases in colorectal pressure. No tachyphylaxis was observed, and the responses were blocked by an NK receptor antagonist (GR159897, 1 mg/kg i.v.). No obvious cardiorespiratory effects were noted. These results suggest that rapid-onset, short-duration, drug-induced voiding is possible in acute spinal and intact rats with intravenous administration of an NK receptor agonist. Future challenges remain in regard to finding alternative routes of administration that produce clinically significant voiding, multiple times per day, in animal models of chronic SCI.
[Mh] Termos MeSH primário: Colo/efeitos dos fármacos
Motilidade Gastrointestinal/efeitos dos fármacos
Contração Muscular/efeitos dos fármacos
Neurocinina A/análogos & derivados
Fragmentos de Peptídeos/farmacologia
Traumatismos da Medula Espinal/tratamento farmacológico
Bexiga Urinária/efeitos dos fármacos
Urodinâmica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Colo/inervação
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Estudos de Viabilidade
Feminino
Indóis/farmacologia
Neurocinina A/farmacologia
Piperidinas/farmacologia
Pressão
Ratos Sprague-Dawley
Receptores da Neurocinina-2/efeitos dos fármacos
Receptores da Neurocinina-2/metabolismo
Traumatismos da Medula Espinal/metabolismo
Traumatismos da Medula Espinal/fisiopatologia
Fatores de Tempo
Bexiga Urinária/inervação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (GR 159897); 0 (Indoles); 0 (Peptide Fragments); 0 (Piperidines); 0 (Receptors, Neurokinin-2); 0 (neurokinin A (4-10), Lys(5)-MeLeu(9)-Nle(10)-); 86933-74-6 (Neurokinin A)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE
[do] DOI:10.1007/s00210-016-1317-4


  3 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27196574
[Au] Autor:Tack J; Schumacher K; Tonini G; Scartoni S; Capriati A; Maggi CA; and the Iris-2 investigators
[Ad] Endereço:Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.
[Ti] Título:The neurokinin-2 receptor antagonist ibodutant improves overall symptoms, abdominal pain and stool pattern in female patients in a phase II study of diarrhoea-predominant IBS.
[So] Source:Gut;66(8):1403-1413, 2017 Aug.
[Is] ISSN:1468-3288
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tachykinins have been implicated in the pathophysiology of IBS with diarrhoea (IBS-D). Our aim was to study the efficacy and safety of ibodutant, a selective neurokinin-2 (NK2) receptor antagonist, in patients with IBS-D. METHODS: This multinational double-blind, placebo-controlled study recruited 559 patients with IBS-D according to Rome III criteria. After a 2-week treatment-free run-in, patients were randomised to ibodutant 1 mg, 3 mg, 10 mg or placebo once daily for eight consecutive weeks. Responders were those with a combined response of satisfactory relief (weekly binary question yes/no) of overall IBS symptoms and abdominal pain/discomfort on ≥75% weeks (primary end point). Secondary end points included abdominal pain and stool pattern. Data were also analysed according to US Food and Drug Administration (FDA)-approved interim end points (improvement of pain and stool consistency). Safety was assessed by monitoring adverse events and laboratory tests. Prespecified statistical analysis involved the whole group as well as gender subgroups. RESULTS: Demographics and baseline characteristics were comparable for all treatment arms. In the overall population, responsiveness tended to increase with escalating ibodutant doses. In the prespecified analysis by gender, ibodutant 10 mg demonstrated significant superiority over placebo in females (p=0.003), while no significant effect occurred in males. This was confirmed for secondary end points and for the responder analysis according to FDA-approved end points. The tolerability and safety of ibodutant was excellent at all doses. CONCLUSIONS: Ibodutant showed dose-dependent efficacy response in IBS-D, reaching statistical significance at the 10 mg dose in female patients. The safety and tolerability profile of ibodutant was similar to placebo. TRIAL REGISTRATION NUMBER: NCT01303224.
[Mh] Termos MeSH primário: Dipeptídeos/uso terapêutico
Fármacos Gastrointestinais/uso terapêutico
Síndrome do Intestino Irritável/tratamento farmacológico
Tiofenos/uso terapêutico
[Mh] Termos MeSH secundário: Dor Abdominal/etiologia
Adolescente
Adulto
Idoso
Diarreia/etiologia
Dipeptídeos/efeitos adversos
Relação Dose-Resposta a Droga
Método Duplo-Cego
Feminino
Fármacos Gastrointestinais/efeitos adversos
Seres Humanos
Síndrome do Intestino Irritável/complicações
Síndrome do Intestino Irritável/fisiopatologia
Masculino
Meia-Idade
Qualidade de Vida
Receptores da Neurocinina-2/antagonistas & inibidores
Fatores Sexuais
Avaliação de Sintomas
Tiofenos/efeitos adversos
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE II; JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Dipeptides); 0 (Gastrointestinal Agents); 0 (Receptors, Neurokinin-2); 0 (Thiophenes); 1H7RSQ28BJ (ibodutant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1136/gutjnl-2015-310683


  4 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27456549
[Au] Autor:González-Santana A; Marrero-Hernández S; Dorta I; Hernández M; Pinto FM; Báez D; Bello AR; Candenas L; Almeida TA
[Ad] Endereço:Unidad de Farmacología, Facultad de Ciencias de la Salud, Universidad de La Laguna, Campus de Ofra s/n, Tenerife, Spain.
[Ti] Título:Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata.
[So] Source:Fertil Steril;106(6):1521-1529, 2016 Nov.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To study the expression levels of tachykinins and tachykinin receptors in uterine leiomyomas and matched myometrium. DESIGN: Laboratory study. SETTING: University research laboratories and academic hospital. PATIENT(S): Women undergoing hysterectomy for symptomatic leiomyomas. INTERVENTION(S): Quantitative polymerase chain reaction, immunohistochemistry and Western blot. MAIN OUTCOME MEASURE(S): Expression and tissue immunostaining of substance P, neurokinin A, hemokinin-1, neurokinin 1 receptor full-length (NK1R-Fl) and truncated (NK1R-Tr) isoforms, and neurokinin 2 receptor (NK2R) in paired samples of leiomyoma and adjacent normal myometrium. RESULT(S): TAC1 messenger RNA (mRNA) was significantly up-regulated in leiomyomas, whereas intense immunoreaction for the three peptides was particularly abundant in connective tissue cells. Differential regulation of TACR1 mRNA was observed, and at the protein level there was a significant increased expression of NK1R short isoform (NK1R-Tr). TACR2 mRNA was significantly up-regulated in leiomyomas, although levels of NK2R protein were similar in normal and tumor cells. CONCLUSION(S): These and our previous data demonstrate that the whole tachykinin system is differentially regulated in leiomyomas. The increased expression of NK1R-Tr might stimulate leiomyoma growth in a similar way to that observed in other steroid-dependent tumors.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/análise
Leiomioma/química
Neurocinina A/análise
Receptores da Neurocinina-1/análise
Receptores da Neurocinina-2/análise
Substância P/análise
Taquicininas/análise
Neoplasias Uterinas/química
[Mh] Termos MeSH secundário: Adulto
Biomarcadores Tumorais/genética
Western Blotting
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Imuno-Histoquímica
Leiomioma/genética
Leiomioma/patologia
Leiomioma/cirurgia
Meia-Idade
Neurocinina A/genética
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptores da Neurocinina-1/genética
Receptores da Neurocinina-2/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Substância P/genética
Taquicininas/genética
Neoplasias Uterinas/genética
Neoplasias Uterinas/patologia
Neoplasias Uterinas/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (RNA, Messenger); 0 (Receptors, Neurokinin-1); 0 (Receptors, Neurokinin-2); 0 (TAC4 protein, human); 0 (Tachykinins); 33507-63-0 (Substance P); 86933-74-6 (Neurokinin A)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE


  5 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27434719
[Au] Autor:Urbanek K; De Angelis A; Spaziano G; Piegari E; Matteis M; Cappetta D; Esposito G; Russo R; Tartaglione G; De Palma R; Rossi F; D'Agostino B
[Ad] Endereço:Department of Experimental Medicine, Section of Pharmacology, Second University of Naples, Naples, Italy.
[Ti] Título:Intratracheal Administration of Mesenchymal Stem Cells Modulates Tachykinin System, Suppresses Airway Remodeling and Reduces Airway Hyperresponsiveness in an Animal Model.
[So] Source:PLoS One;11(7):e0158746, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The need for new options for chronic lung diseases promotes the research on stem cells for lung repair. Bone marrow-derived mesenchymal stem cells (MSCs) can modulate lung inflammation, but the data on cellular processes involved in early airway remodeling and the potential involvement of neuropeptides are scarce. OBJECTIVES: To elucidate the mechanisms by which local administration of MSCs interferes with pathophysiological features of airway hyperresponsiveness in an animal model. METHODS: GFP-tagged mouse MSCs were intratracheally delivered in the ovalbumin mouse model with subsequent functional tests, the analysis of cytokine levels, neuropeptide expression and histological evaluation of MSCs fate and airway pathology. Additionally, MSCs were exposed to pro-inflammatory factors in vitro. RESULTS: Functional improvement was observed after MSC administration. Although MSCs did not adopt lung cell phenotypes, cell therapy positively affected airway remodeling reducing the hyperplastic phase of the gain in bronchial smooth muscle mass, decreasing the proliferation of epithelium in which mucus metaplasia was also lowered. Decrease of interleukin-4, interleukin-5, interleukin-13 and increase of interleukin-10 in bronchoalveolar lavage was also observed. Exposed to pro-inflammatory cytokines, MSCs upregulated indoleamine 2,3-dioxygenase. Moreover, asthma-related in vivo upregulation of pro-inflammatory neurokinin 1 and neurokinin 2 receptors was counteracted by MSCs that also determined a partial restoration of VIP, a neuropeptide with anti-inflammatory properties. CONCLUSION: Intratracheally administered MSCs positively modulate airway remodeling, reduce inflammation and improve function, demonstrating their ability to promote tissue homeostasis in the course of experimental allergic asthma. Because of a limited tissue retention, the functional impact of MSCs may be attributed to their immunomodulatory response combined with the interference of neuropeptide system activation and tissue remodeling.
[Mh] Termos MeSH primário: Transplante de Células-Tronco Mesenquimais
Células Mesenquimais Estromais/imunologia
Receptores da Neurocinina-1/imunologia
Receptores da Neurocinina-2/imunologia
Hipersensibilidade Respiratória/terapia
[Mh] Termos MeSH secundário: Animais
Líquido da Lavagem Broncoalveolar/química
Líquido da Lavagem Broncoalveolar/imunologia
Expressão Gênica
Genes Reporter
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Indolamina-Pirrol 2,3,-Dioxigenase/genética
Indolamina-Pirrol 2,3,-Dioxigenase/imunologia
Interleucina-10/genética
Interleucina-10/imunologia
Interleucina-13/genética
Interleucina-13/imunologia
Interleucina-4/genética
Interleucina-4/imunologia
Interleucina-5/genética
Interleucina-5/imunologia
Intubação Intratraqueal
Pulmão/imunologia
Pulmão/patologia
Células Mesenquimais Estromais/citologia
Camundongos
Camundongos Endogâmicos BALB C
Ovalbumina
Receptores da Neurocinina-1/genética
Receptores da Neurocinina-2/genética
Hipersensibilidade Respiratória/induzido quimicamente
Hipersensibilidade Respiratória/imunologia
Hipersensibilidade Respiratória/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IL10 protein, mouse); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 0 (Interleukin-13); 0 (Interleukin-5); 0 (Receptors, Neurokinin-1); 0 (Receptors, Neurokinin-2); 130068-27-8 (Interleukin-10); 147336-22-9 (Green Fluorescent Proteins); 207137-56-2 (Interleukin-4); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0158746


  6 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27199181
[Au] Autor:Kaczynska K; Jampolska M; Szereda-Przestaszewska M
[Ad] Endereço:Laboratory of Respiration Physiology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
[Ti] Título:The role of vagal pathway and NK1 and NK2 receptors in cardiovascular and respiratory effects of neurokinin A.
[So] Source:Clin Exp Pharmacol Physiol;43(9):818-24, 2016 Sep.
[Is] ISSN:1440-1681
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Neurokinin A (NKA) is a peptide neurotransmitter that participates in the regulation of breathing and the cardiovascular system. The purpose of the current study was to determine the cardiorespiratory pattern exerted by the systemic injection of NKA, to look at the contribution of neurokinin NK1 and NK2 receptors, and to establish the engagement of the vagal pathway in mediation of these responses. The effects of intravenous injections of NKA (50 µg/kg) were studied in anaesthetized, spontaneously breathing rats in the following experimental schemes: in neurally intact rats; and vagotomized at either midcervical or supranodosal level. Intravenous injections of NKA in the intact rats evoked sudden and short-lived increase in the respiratory rate concomitant with drop in tidal volume, followed by a prolonged depression, coupled with continuous augmentation of the tidal volume. Respiratory alterations were accompanied by transient tachycardia and prolonged hypotension. Midcervical vagotomy eliminated respiratory rate response and augmentation of tidal volume. Section of supranodosal vagi abrogated all respiratory reactions. NK2 receptor blockade abolished respiratory changes without affecting cardiovascular effects, whereas NK1 receptor blockade significantly reduced hypotension and increase in heart rate with no impact on the respiratory system. These results indicate that NKA induced changes in the breathing resulting from an excitation of the NK2 receptors on the vagal endings. A fall in blood pressure triggered by NKA occurs outside of the vagus nerve and is probably mediated via its direct action on vascular smooth muscles supplied with NK1 receptors.
[Mh] Termos MeSH primário: Pressão Sanguínea/efeitos dos fármacos
Neurocinina A/farmacologia
Receptores da Neurocinina-1/metabolismo
Receptores da Neurocinina-2/metabolismo
Fenômenos Fisiológicos Respiratórios/efeitos dos fármacos
Nervo Vago/efeitos dos fármacos
Nervo Vago/metabolismo
[Mh] Termos MeSH secundário: Animais
Masculino
Antagonistas do Receptor de Neuroquinina-1/farmacologia
Ratos
Ratos Wistar
Receptores da Neurocinina-2/antagonistas & inibidores
Nervo Vago/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurokinin-1 Receptor Antagonists); 0 (Receptors, Neurokinin-1); 0 (Receptors, Neurokinin-2); 86933-74-6 (Neurokinin A)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160521
[St] Status:MEDLINE
[do] DOI:10.1111/1440-1681.12594


  7 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26851827
[Au] Autor:Lu P; Luo H; Quan X; Fan H; Tang Q; Yu G; Chen W; Xia H
[Ad] Endereço:Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
[Ti] Título:The role of substance P in the maintenance of colonic hypermotility induced by repeated stress in rats.
[So] Source:Neuropeptides;56:75-82, 2016 Apr.
[Is] ISSN:1532-2785
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The mechanism underlying chronic stress-induced gastrointestinal (GI) dysmotility has not been fully elucidated and GI hormones have been indicated playing a role in mediating stress-induced changes in GI motor function. AIMS: Our objective was to study the possible role of substance P (SP) in the colonic hypermotility induced by repeated water avoidance stress (WAS) which mimics irritable bowel syndrome. METHODS: Male Wistar rats were submitted to WAS or sham WAS (SWAS) (1h/day) for up to 10 consecutive days. Enzyme Immunoassay Kit was used to detect the serum level of SP. The expression of neurokinin-1 receptor (NK1R) was investigated by Immunohistochemistry and Western blotting. The spontaneous contraction of muscle strip was studied in an organ bath system. L-type calcium channel currents (ICa,L) of smooth muscle cells (SMCs) were recorded by whole-cell patch-clamp technique. RESULTS: Fecal pellet expulsion and spontaneous contraction of proximal colon in rats were increased after repeated WAS. The serum level of SP was elevated following WAS. Immunohistochemistry proved the expression of NK1R in mucosa, muscularis and myenteric plexus. Western blotting demonstrated stress-induced up-regulation of NK1R in colon devoid of mucosa and submucosa. Repeated WAS increased the contractile activities of longitudinal muscle and circular muscle strips induced by SP and this effect was reversed by a selective NK1R antagonist. The ICa,L of SMCs in the WAS rats were drastically increased compared to controls after addition of SP. CONCLUSIONS: Increased serum SP level and up-regulated NK1R in colon may contribute to stress-induced colonic hypermotility. And L-type calcium channels play a potentially important role in the process of WAS-induced dysmotility.
[Mh] Termos MeSH primário: Colo/fisiopatologia
Motilidade Gastrointestinal
Estresse Psicológico/sangue
Estresse Psicológico/fisiopatologia
Substância P/sangue
[Mh] Termos MeSH secundário: Animais
Canais de Cálcio Tipo L/metabolismo
Colo/metabolismo
Masculino
Músculo Liso/metabolismo
Músculo Liso/fisiopatologia
Plexo Mientérico/metabolismo
Ratos
Ratos Wistar
Receptores da Neurocinina-1/metabolismo
Receptores da Neurocinina-2/metabolismo
Estresse Psicológico/complicações
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Calcium Channels, L-Type); 0 (Receptors, Neurokinin-1); 0 (Receptors, Neurokinin-2); 33507-63-0 (Substance P)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160208
[St] Status:MEDLINE


  8 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26758701
[Au] Autor:Bellucci F; Buéno L; Bugianesi R; Crea A; D'Aranno V; Meini S; Santicioli P; Tramontana M; Maggi CA
[Ad] Endereço:Pharmacology Department, Menarini Ricerche S.p.A., Florence, Italy.
[Ti] Título:Gender-related differential effect of tachykinin NK2 receptor-mediated visceral hyperalgesia in guinea pig colon.
[So] Source:Br J Pharmacol;173(8):1329-38, 2016 Apr.
[Is] ISSN:1476-5381
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: The tachykinin NK2 receptor antagonist ibodutant is under Phase III clinical investigation to treat female patients with irritable bowel syndrome. The aim of this study was to investigate the NK2 receptor-related gender specificity in a model of colitis. EXPERIMENTAL APPROACH: Colitis was induced by rectal instillation of 2,4,6-trinitrobenzenesulfonic acid (TNBS, 0.5 mL, 30 mg·mL(-1) in 30% ethanol) in female and male guinea pigs. Electromyographic recording of the responses to colorectal distension (CRD) was made 3 days later. Ibodutant (0.33 , 0.65, 1.9 and 6.5 mg·kg(-1) ) was given s.c., 30 min before CRD. Release of neurokinin A and substance P from isolated mucosal and smooth muscle tissues following treatment with KCl (80 mM) or capsaicin (10 µM) was measured by EIA. Plasma pharmacokinetics of ibodutant following a single s.c. administration (0.73 or 2.1 mg·kg(-1) ) were measured over 24 h. KEY RESULTS: Ibodutant did not affect abdominal contractions in control animals. After TNBS-induced colitis, ibodutant prevented the increased visceral hypersensitivity to CRD in females, at lower doses than in males. Ibodutant pharmacokinetics did not differ between females and males. Tachykinins release was greater in smooth muscle than in mucosal samples. Capsaicin-stimulated release of tachykinins from inflamed mucosal samples from females was significantly lower than in males. CONCLUSIONS AND IMPLICATIONS: Ibodutant prevented abdominal nociception in a model of visceral hypersensitivity in guinea pigs with a greater efficacy in females than in males. Our results highlight a gender-related difference in colonic visceral hypersensitivity and mucosal nerve activation.
[Mh] Termos MeSH primário: Colite/metabolismo
Colo/metabolismo
Hiperalgesia/metabolismo
Receptores da Neurocinina-2/metabolismo
Caracteres Sexuais
Dor Visceral/metabolismo
[Mh] Termos MeSH secundário: Animais
Colite/induzido quimicamente
Colite/prevenção & controle
Dipeptídeos/administração & dosagem
Dipeptídeos/sangue
Dipeptídeos/farmacologia
Modelos Animais de Doenças
Feminino
Cobaias
Hiperalgesia/prevenção & controle
Masculino
Tiofenos/administração & dosagem
Tiofenos/sangue
Tiofenos/farmacologia
Ácido Trinitrobenzenossulfônico
Dor Visceral/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dipeptides); 0 (Receptors, Neurokinin-2); 0 (Thiophenes); 1H7RSQ28BJ (ibodutant); 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160114
[St] Status:MEDLINE
[do] DOI:10.1111/bph.13427


  9 / 1092 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Antunes, Edson
Texto completo
[PMID]:26607257
[Au] Autor:Rocha-E-Silva TA; Linardi A; Antunes E; Hyslop S
[Ad] Endereço:Departamento de Farmacologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, Campinas, São Paulo, Brazil (T.A.A.R.S., E.A., S.H.); and Departamento de Ciências Fisiológicas, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, Bra
[Ti] Título:Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats.
[So] Source:J Pharmacol Exp Ther;356(1):13-9, 2016 Jan.
[Is] ISSN:1521-0103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 µg/site) edema in rat dorsal skin. This edema was significantly inhibited by ((S)1-{2-[3(3-4-dichlorophenyl)-1-(3-iso-propoxyphenylacetyl)piperidine-3-yl]ethyl}-4-phenyl-1-azoniabicyclo[2.2.2]octone, chloride) (SR140333, a neurokinin NK1 receptor antagonist), indomethacin [a nonselective cyclooxygenase (COX) inhibitor], cyproheptadine (a serotonin 5-hydroxytryptamine1/2 and histamine H1 receptor antagonist), and N(ω)-nitro-L-arginine methyl ester (a nitric oxide synthase inhibitor). In contrast, mepyramine (a histamine H1 receptor antagonist), D-Arg-[Hyp(3),Thi(5),D-Tic(7),Oic(8)-]-BK (JE 049, a bradykinin B2 receptor antagonist), and ((S)-N-methyl-N-[4-(4-acetylamino-4-phenylpiperidino)-2-(3,4-di-chlorophenyl)butyl]benzamide) (SR48968, a neurokinin NK2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.
[Mh] Termos MeSH primário: Edema/induzido quimicamente
Edema/patologia
Piperidinas/uso terapêutico
Quinuclidinas/uso terapêutico
Venenos de Aranha/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/uso terapêutico
Inibidores de Ciclo-Oxigenase/uso terapêutico
Ciproeptadina/uso terapêutico
Relação Dose-Resposta a Droga
Edema/tratamento farmacológico
/patologia
Antagonistas dos Receptores Histamínicos H1/uso terapêutico
Indometacina/uso terapêutico
Cetoprofeno/uso terapêutico
Masculino
NG-Nitroarginina Metil Éster/uso terapêutico
Antagonistas do Receptor de Neuroquinina-1/uso terapêutico
Óxido Nítrico Sintase/antagonistas & inibidores
Ratos
Ratos Wistar
Receptores da Neurocinina-2/efeitos dos fármacos
Antagonistas da Serotonina/uso terapêutico
Pele/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cyclooxygenase Inhibitors); 0 (Histamine H1 Antagonists); 0 (Neurokinin-1 Receptor Antagonists); 0 (Piperidines); 0 (Quinuclidines); 0 (Receptors, Neurokinin-2); 0 (Serotonin Antagonists); 0 (Spider Venoms); 153050-21-6 (SR 140333); 2YHB6175DO (Cyproheptadine); 90Y4QC304K (Ketoprofen); EC 1.14.13.39 (Nitric Oxide Synthase); V55S2QJN2X (NG-Nitroarginine Methyl Ester); XXE1CET956 (Indomethacin)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:151126
[Lr] Data última revisão:
151126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151127
[St] Status:MEDLINE
[do] DOI:10.1124/jpet.115.226787


  10 / 1092 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26524655
[Au] Autor:Zhao L; Zhuang J; Zang N; Lin Y; Lee LY; Xu F
[Ad] Endereço:Pathophysiology Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA.
[Ti] Título:Prenatal nicotinic exposure upregulates pulmonary C-fiber NK1R expression to prolong pulmonary C-fiber-mediated apneic response.
[So] Source:Toxicol Appl Pharmacol;290:107-15, 2016 Jan 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prenatal nicotinic exposure (PNE) prolongs bronchopulmonary C-fiber (PCF)-mediated apneic response to intra-atrial bolus injection of capsaicin in rat pups. The relevant mechanisms remain unclear. Pulmonary substance P and adenosine and their receptors (neurokinin-A receptor, NK1R and ADA1 receptor, ADA1R) and transient receptor potential cation channel subfamily V member 1 (TRPV1) expressed on PCFs are critical for PCF sensitization and/or activation. Here, we compared substance P and adenosine in BALF and NK1R, ADA1R, and TRPV1 expression in the nodose/jugular (N/J) ganglia (vagal pulmonary C-neurons retrogradely labeled) between Ctrl and PNE pups. We found that PNE failed to change BALF substance P and adenosine content, but significantly upregulated both mRNA and protein TRPV1 and NK1R in the N/J ganglia and only NK1R mRNA in pulmonary C-neurons. To define the role of NK1R in the PNE-induced PCF sensitization, the apneic response to capsaicin (i.v.) without or with pretreatment of SR140333 (a peripheral and selective NK1R antagonist) was compared and the prolonged apnea by PNE significantly shortened by SR140333. To clarify if the PNE-evoked responses depended on action of nicotinic acetylcholine receptors (nAChRs), particularly α7nAChR, mecamylamine or methyllycaconitine (a general nAChR or a selective α7nAChR antagonist) was administrated via another mini-pump over the PNE period. Mecamylamine or methyllycaconitine eliminated the PNE-evoked mRNA and protein responses. Our data suggest that PNE is able to elevate PCF NK1R expression via activation of nAChRs, especially α7nAChR, which likely contributes to sensitize PCFs and prolong the PCF-mediated apneic response to capsaicin.
[Mh] Termos MeSH primário: Pulmão/efeitos dos fármacos
Fibras Nervosas Amielínicas/efeitos dos fármacos
Receptores da Neurocinina-1/metabolismo
Receptores da Neurocinina-2/metabolismo
Regulação para Cima
[Mh] Termos MeSH secundário: Adenosina/farmacologia
Animais
Animais Recém-Nascidos
Apneia/tratamento farmacológico
Líquido da Lavagem Broncoalveolar
Capsaicina/farmacologia
Feminino
Gânglios/efeitos dos fármacos
Gânglios/metabolismo
Masculino
Fibras Nervosas Amielínicas/metabolismo
Nicotina/sangue
Nicotina/toxicidade
Gravidez
Efeitos Tardios da Exposição Pré-Natal
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Ratos
Ratos Sprague-Dawley
Receptores da Neurocinina-1/genética
Receptores da Neurocinina-2/genética
Substância P/farmacologia
Canais de Cátion TRPV/genética
Canais de Cátion TRPV/metabolismo
Nervo Vago/efeitos dos fármacos
Nervo Vago/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Receptors, Neurokinin-1); 0 (Receptors, Neurokinin-2); 0 (TRPV Cation Channels); 0 (Trpv1 protein, rat); 33507-63-0 (Substance P); 6M3C89ZY6R (Nicotine); K72T3FS567 (Adenosine); S07O44R1ZM (Capsaicin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151103
[St] Status:MEDLINE



página 1 de 110 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde