Base de dados : MEDLINE Pesquisa : D12.776.543.750.705.408.200.800 [Categoria DeCS] Referências encontradas : 490 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 49

ir para página

1 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28772128 [Au] Autor: Lohneis P; Sinn M; Bischoff S; Jühling A; Pelzer U; Wislocka L; Bahra M; Sinn BV; Denkert C; Oettle H; Bläker H; Riess H; Jöhrens K; Striefler JK [Ad] Endereço: Universitätsmedizin Charité Berlin, Institute of Pathology, Charitéplatz 1, 10117 Berlin, Germany. Electronic address: philipp.lohneis@charite.de. [Ti] Título: Cytotoxic tumour-infiltrating T lymphocytes influence outcome in resected pancreatic ductal adenocarcinoma. [So] Source: Eur J Cancer;83:290-301, 2017 Sep. [Is] ISSN: 1879-0852 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: We studied the prognostic effect of CD3-, CD8- and CD103-positive T lymphocytes in a cohort of 165 patients with resected pancreatic ductal adenocarcinomas (PDACs) of the treatment group (adjuvant gemcitabine) and the untreated control group of the CONKO-001 study. METHODS: Immunohistochemical stainings on tissue microarrays (TMAs) against CD3, CD8 and CD103 were performed according to standard procedures. RESULTS: A high number of CD8-positive lymphocytes were significantly and independently associated with longer disease-free survival (DFS) and overall survival (OS) in the overall study population. Median DFS/OS were 7.4/18.1 months for patients with a low number of CD8-positive intratumoural lymphocytes (≤42 per 1 mm tissue core) and 12.7/25.2 months for patients with high numbers (>42 per 1-mm tissue core; p = 0.008/0.020; HR 0.62/0.65). The ratio of intraepithelial to total CD103-positive lymphocytes, but not total numbers of CD103-positive lymphocytes or CD103-positive intraepithelial lymphocytes, was associated with significantly improved DFS and OS in the overall study population (p = 0.022/0.009). Median DFS/OS was 5.9/15.7 for patients with a ratio of intraepithelial to total CD103-positive intratumoural lymphocytes higher than 0.3 and 11.6/24.7 for patients with a lower ratio. CONCLUSION: T-lymphocyte subpopulations might be prognostic in resectable PDAC but need standardization and verification by further studies. [Mh] Termos MeSH primário: Linfócitos T CD8-Positivos/imunologia Carcinoma Ductal Pancreático/imunologia Linfócitos do Interstício Tumoral/imunologia Neoplasias Pancreáticas/imunologia Linfócitos T Citotóxicos/imunologia [Mh] Termos MeSH secundário: Adulto Idoso Idoso de 80 Anos ou mais Antimetabólitos Antineoplásicos/uso terapêutico Complexo CD3/metabolismo Carcinoma Ductal Pancreático/patologia Carcinoma Ductal Pancreático/terapia Desoxicitidina/análogos & derivados Desoxicitidina/uso terapêutico Intervalo Livre de Doença Feminino Seres Humanos Integrina beta4/análise Masculino Meia-Idade Neoplasias Pancreáticas/patologia Neoplasias Pancreáticas/terapia Prognóstico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antimetabolites, Antineoplastic); 0 (CD3 Complex); 0 (Integrin beta4); 0W860991D6 (Deoxycytidine); B76N6SBZ8R (gemcitabine) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170804 [St] Status: MEDLINE

2 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28414312 [Au] Autor: Porpiglia E; Samusik N; Van Ho AT; Cosgrove BD; Mai T; Davis KL; Jager A; Nolan GP; Bendall SC; Fantl WJ; Blau HM [Ad] Endereço: Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA. [Ti] Título: High-resolution myogenic lineage mapping by single-cell mass cytometry. [So] Source: Nat Cell Biol;19(5):558-567, 2017 May. [Is] ISSN: 1476-4679 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Muscle regeneration is a dynamic process during which cell state and identity change over time. A major roadblock has been a lack of tools to resolve a myogenic progression in vivo. Here we capitalize on a transformative technology, single-cell mass cytometry (CyTOF), to identify in vivo skeletal muscle stem cell and previously unrecognized progenitor populations that precede differentiation. We discovered two cell surface markers, CD9 and CD104, whose combined expression enabled in vivo identification and prospective isolation of stem and progenitor cells. Data analysis using the X-shift algorithm paired with single-cell force-directed layout visualization defined a molecular signature of the activated stem cell state (CD44 /CD98 /MyoD ) and delineated a myogenic trajectory during recovery from acute muscle injury. Our studies uncover the dynamics of skeletal muscle regeneration in vivo and pave the way for the elucidation of the regulatory networks that underlie cell-state transitions in muscle diseases and ageing. [Mh] Termos MeSH primário: Linhagem da Célula Separação Celular/métodos Citometria de Fluxo/métodos Desenvolvimento Muscular Músculo Esquelético/metabolismo Mioblastos Esqueléticos/metabolismo Regeneração Análise de Célula Única/métodos Células-Tronco/metabolismo [Mh] Termos MeSH secundário: Animais Biomarcadores/metabolismo Proliferação Celular Células Cultivadas Venenos Elapídicos/toxicidade Proteína-1 Reguladora de Fusão/metabolismo Genes Reporter Genótipo Ensaios de Triagem em Larga Escala Receptores de Hialuronatos/metabolismo Integrina beta4/metabolismo Proteínas Luminescentes/genética Proteínas Luminescentes/metabolismo Camundongos Endogâmicos C57BL Camundongos Transgênicos Desenvolvimento Muscular/efeitos dos fármacos Músculo Esquelético/efeitos dos fármacos Músculo Esquelético/lesões Músculo Esquelético/patologia Proteína MyoD/metabolismo Mioblastos Esqueléticos/efeitos dos fármacos Mioblastos Esqueléticos/patologia Fator de Transcrição PAX7/deficiência Fator de Transcrição PAX7/genética Fenótipo Regeneração/efeitos dos fármacos Células-Tronco/efeitos dos fármacos Células-Tronco/patologia Tetraspanina-29/metabolismo Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Cd44 protein, mouse); 0 (Cd9 protein, mouse); 0 (Elapid Venoms); 0 (Fusion Regulatory Protein-1); 0 (Hyaluronan Receptors); 0 (Integrin beta4); 0 (Luminescent Proteins); 0 (MyoD Protein); 0 (MyoD1 myogenic differentiation protein); 0 (PAX7 Transcription Factor); 0 (Pax7 protein, mouse); 0 (Tetraspanin-29); 37223-96-4 (notexin) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170418 [St] Status: MEDLINE [do] DOI: 10.1038/ncb3507

3 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28300755 [Au] Autor: Garimella R; Tadikonda P; Tawfik O; Gunewardena S; Rowe P; Van Veldhuizen P [Ad] Endereço: Division of Medical Clinical Oncology, The University of Kansas Medical Center, Kansas City, KS 66160, USA. Rama.Garimella@va.gov. [Ti] Título: Vitamin D Impacts the Expression of Runx2 Target Genes and Modulates Inflammation, Oxidative Stress and Membrane Vesicle Biogenesis Gene Networks in 143B Osteosarcoma Cells. [So] Source: Int J Mol Sci;18(3), 2017 Mar 16. [Is] ISSN: 1422-0067 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Osteosarcoma (OS) is an aggressive malignancy of bone affecting children, adolescents and young adults. Understanding vitamin D metabolism and vitamin D regulated genes in OS is an important aspect of vitamin D/cancer paradigm, and in evaluating vitamin D as adjuvant therapy for human OS. Vitamin D treatment of 143B OS cells induced significant and novel changes in the expression of genes that regulate: (a) inflammation and immunity; (b) formation of reactive oxygen species, metabolism of cyclic nucleotides, sterols, vitamins and mineral (calcium), quantity of gap junctions and skeletogenesis; (c) bone mineral density; and (d) cell viability of skeletal cells, aggregation of bone cancer cells and exocytosis of secretory vesicles. Ingenuity pathway analysis revealed significant reduction in Runx2 target genes such as fibroblast growth factor -1, -12 ( and ), bone morphogenetic factor-1 ( ), SWI/SNF related, matrix associated actin dependent regulator of chromatin subfamily a, member 4 ( ), Matrix extracellular phosphoglycoprotein ( ), Integrin, ß4 ( ), Matrix Metalloproteinase -1, -28 ( and ), and signal transducer and activator of transcription-4 ( ) in vitamin D treated 143B OS cells. These genes interact with the inflammation, oxidative stress and membrane vesicle biogenesis gene networks. Vitamin D not only inhibited the expression of Runx2 target genes , and kallikrein related peptidase-7 ( ), but also migration and invasion of 143B OS cells. Vitamin D regulated Runx2 target genes or their products represent potential therapeutic targets and laboratory biomarkers for applications in translational oncology. [Mh] Termos MeSH primário: Subunidade alfa 1 de Fator de Ligação ao Core/genética Redes Reguladoras de Genes Osteossarcoma/metabolismo Estresse Oxidativo Vesículas Transportadoras/genética Vitamina D/farmacologia Vitaminas/farmacologia [Mh] Termos MeSH secundário: Proteína Morfogenética Óssea 1/genética Proteína Morfogenética Óssea 1/metabolismo Linhagem Celular Tumoral Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo DNA Helicases/genética DNA Helicases/metabolismo Proteínas da Matriz Extracelular/genética Proteínas da Matriz Extracelular/metabolismo Fatores de Crescimento de Fibroblastos/genética Fatores de Crescimento de Fibroblastos/metabolismo Glicoproteínas/genética Glicoproteínas/metabolismo Seres Humanos Inflamação/genética Integrina beta4/genética Integrina beta4/metabolismo Calicreínas/genética Calicreínas/metabolismo Metaloproteinases da Matriz/genética Metaloproteinases da Matriz/metabolismo Proteínas Nucleares/genética Proteínas Nucleares/metabolismo Fosfoproteínas/genética Fosfoproteínas/metabolismo Fatores de Transcrição/genética Fatores de Transcrição/metabolismo Vesículas Transportadoras/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Core Binding Factor Alpha 1 Subunit); 0 (Extracellular Matrix Proteins); 0 (Glycoproteins); 0 (Integrin beta4); 0 (MEPE protein, human); 0 (Nuclear Proteins); 0 (Phosphoproteins); 0 (RUNX2 protein, human); 0 (Transcription Factors); 0 (Vitamins); 1406-16-2 (Vitamin D); 62031-54-3 (Fibroblast Growth Factors); EC 3.4.21.- (KLK7 protein, human); EC 3.4.21.- (Kallikreins); EC 3.4.24.- (Matrix Metalloproteinases); EC 3.4.24.19 (BMP1 protein, human); EC 3.4.24.19 (Bone Morphogenetic Protein 1); EC 3.6.1.- (SMARCA4 protein, human); EC 3.6.4.- (DNA Helicases) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170420 [Lr] Data última revisão: 170420 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170317 [St] Status: MEDLINE

4 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28287822 [Au] Autor: Sinha M; Lowell CA [Ad] Endereço: Department of Laboratory Medicine and Program in Immunology, University of California, San Francisco, California. [Ti] Título: Efficiency and Specificity of Gene Deletion in Lung Epithelial Doxycycline-Inducible Cre Mice. [So] Source: Am J Respir Cell Mol Biol;57(2):248-257, 2017 Aug. [Is] ISSN: 1535-4989 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The transgenic mouse strains surfactant protein C-reverse tetracycline transactivator (SP-C-rtTA), club cell secretory protein (CCSP)-rtTA, and tetracycline operator (TetO)-Cre have been invaluable for spatiotemporally regulating gene deletion in the pulmonary epithelium. In this study, we measured the efficiency and specificity of gene deletion that can be achieved in these mice using the Rosa26-eYFP reporter. Triple-transgenic mice (tTg or rtTA/TetO-Cre/Rosa-eYFP) were bred and treated with various doxycycline (dox) regimens to induce gene deletion, which was then quantified in various cell populations by flow cytometry. In these crosses, we found that the TetO-Cre transgene must be transmitted through the female parent to avoid germline gene deletion. With dox exposure during lung development, SP-C-tTg mice deleted in ∼65-75% of alveolar epithelial type II (ATII) cells, but in only ∼45-50% of the integrin ß4 population, which consisted of club cells and distal lung progenitor cells. In contrast, CCSP-tTg mice deleted in ∼50% of ATII cells and ∼80% of integrin ß4 cells. Upon dox treatment of adults, deletion in ATII cells and integrin ß4 cells in SP-C-tTg mice dropped significantly to ∼20% and ∼6%, respectively, whereas CCSP-tTg mice deleted in ∼57% of ATII and ∼40% of integrin ß4 cells. Interestingly, untreated CCSP-tTg mice also deleted in ∼40% of integrin ß4 cells, indicating significant leakiness of CCSP-tTg in ß4 cells. In all mouse groups, minimal deletion occurred in mouse tracheal epithelial cells or in mesenchymal or hematopoietic cells. These data provide the first quantitative, side-by-side comparison of the deletion efficiency for these widely used transgenic mouse strains. [Mh] Termos MeSH primário: Células Epiteliais Alveolares/metabolismo Deleção de Genes Técnicas de Inativação de Genes Integrases/genética Pulmão/citologia Camundongos Transgênicos/genética Traqueia/citologia Transgenes [Mh] Termos MeSH secundário: Animais Proteínas de Bactérias/genética Proteínas de Transporte/genética Doxiciclina/farmacologia Feminino Citometria de Fluxo Genes Reporter Integrina beta4/análise Proteínas Luminescentes/genética Pulmão/embriologia Masculino Herança Materna Camundongos Camundongos Endogâmicos C57BL Especificidade de Órgãos Peptídeos/genética Uteroglobina/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (Integrin beta4); 0 (Luminescent Proteins); 0 (Peptides); 0 (Scgb1a1 protein, mouse); 0 (Sftpc protein, mouse); 0 (Tet O resistance protein, Bacteria); 0 (yellow fluorescent protein, Bacteria); 9060-09-7 (Uteroglobin); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases); N12000U13O (Doxycycline) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170919 [Lr] Data última revisão: 170919 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170314 [St] Status: MEDLINE [do] DOI: 10.1165/rcmb.2016-0208OC

5 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28011194 [Au] Autor: Dilly AK; Tang K; Guo Y; Joshi S; Ekambaram P; Maddipati KR; Cai Y; Tucker SC; Honn KV [Ad] Endereço: Departments of Pathology-Bioactive Lipids Research Program, Detroit, MI 48202, United States. Electronic address: akd37@pitt.edu. [Ti] Título: Convergence of eicosanoid and integrin biology: Role of Src in 12-LOX activation. [So] Source: Exp Cell Res;351(1):1-10, 2017 Feb 01. [Is] ISSN: 1090-2422 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: 12-Lipoxygenase (12-LOX) metabolizes arachidonic acid to 12(S)-hydroxyeicosatetraenoic acid, or 12(S)-HETE, a proinflammatory bioactive lipid implicated in tumor angiogenesis, growth, and metastasis. The mechanisms underlying 12-LOX-mediated signaling in cancer progression are still ill-defined. In the present study we demonstrate that 12-LOX phosphorylation and subsequent enzymatic activity occurs after integrin ß4 stimulation and Src kinase recruitment to the integrin subunit. Inhibition of Src activity by PP2 or Src dominant-negative mutants reduced 12-LOX tyrosine phosphorylation and 12(S)-HETE production in response to integrin ß4 stimulation in A431 cells. The pertinent Src-targeted residues for 12-LOX activity were mapped to Y19 and Y614, where 12-LOX mutants Y19F and Y614F showed 70% less enzymatic activity. Furthermore, we have shown that the 12-LOX activity modulated by these residues impacts migration. To our knowledge, this is the first report that c-Src kinase activity is required for ß4-integrin-mediated phosphorylation of 12-LOX. [Mh] Termos MeSH primário: Ácido 12-Hidroxi-5,8,10,14-Eicosatetraenoico/metabolismo Araquidonato 12-Lipoxigenase/metabolismo Movimento Celular Integrina beta4/metabolismo Quinases da Família src/metabolismo [Mh] Termos MeSH secundário: Linhagem Celular Tumoral Células HEK293 Seres Humanos Integrina beta4/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (ITGB4 protein, human); 0 (Integrin beta4); 59985-28-3 (12-Hydroxy-5,8,10,14-eicosatetraenoic Acid); EC 1.13.11.31 (Arachidonate 12-Lipoxygenase); EC 2.7.10.2 (src-Family Kinases) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170518 [Lr] Data última revisão: 170518 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161225 [St] Status: MEDLINE

6 / 490

MEDLINE

seleciona para imprimir Fotocópia

[PMID]: 27763564 [Au] Autor: Acosta KB; Lorenzini Campos MN; Etcheverry SB; Zapata PD [Ad] Endereço: Instituto de Biotecnología Misiones "Dra. Maria EbeReca" (InBioMis), Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Ruta Nacional Nº12 km 7 ½, Posadas 3300, Argentina. acostakb@yahoo.com.ar. [Ti] Título: α6ß4 Integrin Genetic Variations (A380T and R1281W) and Breast Cancer Risk in an Argentinian Population. [So] Source: Int J Mol Sci;17(10), 2016 Oct 18. [Is] ISSN: 1422-0067 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: The α6ß4 integrin is composed of the α6 and ß4 subunits that are encoded by the and the genes, respectively. The α6ß4 main function is to intervene in lamination and epithelia integrity maintenance by cell-matrix interactions. This integrin appears to have importance in breast cancer malignancy, as well as other epithelial carcinomas. The aim of this work was to investigate the potential role of (A380T) and (R1281W) genetic variations in breast cancer susceptibility, in a female population from the northeast region of Argentina (Misiones). We performed a case-control study of 85 breast cancer patients and 113 cancer-free controls. Genotyping was performed by RFLP-PCR. For (A380T) single nucleotide polymorphism, a high frequency of heterozygous genotype GA in cases compared to controls was observed, achieving values of 48% and 49%, respectively. No association between the A380T SNP and breast cancer development was found (Odds Ratio = 0.92; 95% Confidence Interval = 0.52-1.63; = 0.884). In conclusion, we did not find evidence of an association between A380T ( ) and the risk of developing breast cancer. The results represent the first report of these genetic variations in breast cancer; therefore, they are an important contribution to the literature. [Mh] Termos MeSH primário: Neoplasias da Mama/genética Integrina alfa6/genética Integrina beta4/genética Polimorfismo de Nucleotídeo Único [Mh] Termos MeSH secundário: Adulto Idoso Argentina/epidemiologia Mama/metabolismo Mama/patologia Neoplasias da Mama/epidemiologia Neoplasias da Mama/patologia Estudos de Casos e Controles Feminino Frequência do Gene Variação Genética Genótipo Seres Humanos Meia-Idade Razão de Chances Risco [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (ITGA6 protein, human); 0 (ITGB4 protein, human); 0 (Integrin alpha6); 0 (Integrin beta4) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170403 [Lr] Data última revisão: 170403 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161021 [St] Status: MEDLINE

7 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27736988 [Au] Autor: Moriyama M; Moriyama H; Uda J; Kubo H; Nakajima Y; Goto A; Akaki J; Yoshida I; Matsuoka N; Hayakawa T [Ad] Endereço: Pharmaceutical Research and Technology Institute, Kindai University, Higashi-Osaka, Osaka, Japan. [Ti] Título: Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes. [So] Source: PLoS One;11(10):e0164799, 2016. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Aloe has been used as a folk medicine because it has several important therapeutic properties. These include wound and burn healing, and Aloe is now used in a variety of commercially available topical medications for wound healing and skin care. However, its effects on epidermal keratinocytes remain largely unclear. Our data indicated that both Aloe vera gel (AVG) and Cape aloe extract (CAE) significantly improved wound healing in human primary epidermal keratinocytes (HPEKs) and a human skin equivalent model. In addition, flow cytometry analysis revealed that cell surface expressions of ß1-, α6-, ß4-integrin, and E-cadherin increased in HPEKs treated with AVG and CAE. These increases may contribute to cell migration and wound healing. Treatment with Aloe also resulted in significant changes in cell-cycle progression and in increases in cell number. Aloe increased gene expression of differentiation markers in HPEKs, suggesting roles for AVG and CAE in the improvement of keratinocyte function. Furthermore, human skin epidermal equivalents developed from HPEKs with medium containing Aloe were thicker than control equivalents, indicating the effectiveness of Aloe on enhancing epidermal development. Based on these results, both AVG and CAE have benefits in wound healing and in treatment of rough skin. [Mh] Termos MeSH primário: Aloe/química Diferenciação Celular/efeitos dos fármacos Proliferação Celular/efeitos dos fármacos Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Aloe/metabolismo Caderinas/genética Caderinas/metabolismo Linhagem Celular Movimento Celular/efeitos dos fármacos Proteínas Ricas em Prolina do Estrato Córneo/genética Proteínas Ricas em Prolina do Estrato Córneo/metabolismo Seres Humanos Integrina alfa6/genética Integrina alfa6/metabolismo Integrina beta1/genética Integrina beta1/metabolismo Integrina beta4/genética Integrina beta4/metabolismo Queratinócitos/citologia Queratinócitos/efeitos dos fármacos Queratinócitos/metabolismo Microscopia de Fluorescência Modelos Biológicos Extratos Vegetais/química Cicatrização [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cadherins); 0 (Cornified Envelope Proline-Rich Proteins); 0 (Integrin alpha6); 0 (Integrin beta1); 0 (Integrin beta4); 0 (Plant Extracts); 0 (SPRR2A protein, human); 149686-82-8 (SPRR1B protein, human) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161014 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0164799

8 / 490

MEDLINE

seleciona para imprimir Fotocópia PubMed Central Texto completo Texto completo

[PMID]: 27348469 [Au] Autor: Langlands AJ; Almet AA; Appleton PL; Newton IP; Osborne JM; Näthke IS [Ad] Endereço: Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom. [Ti] Título: Paneth Cell-Rich Regions Separated by a Cluster of Lgr5+ Cells Initiate Crypt Fission in the Intestinal Stem Cell Niche. [So] Source: PLoS Biol;14(6):e1002491, 2016 Jun. [Is] ISSN: 1545-7885 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The crypts of the intestinal epithelium house the stem cells that ensure the continual renewal of the epithelial cells that line the intestinal tract. Crypt number increases by a process called crypt fission, the division of a single crypt into two daughter crypts. Fission drives normal tissue growth and maintenance. Correspondingly, it becomes less frequent in adulthood. Importantly, fission is reactivated to drive adenoma growth. The mechanisms governing fission are poorly understood. However, only by knowing how normal fission operates can cancer-associated changes be elucidated. We studied normal fission in tissue in three dimensions using high-resolution imaging and used intestinal organoids to identify underlying mechanisms. We discovered that both the number and relative position of Paneth cells and Lgr5+ cells are important for fission. Furthermore, the higher stiffness and increased adhesion of Paneth cells are involved in determining the site of fission. Formation of a cluster of Lgr5+ cells between at least two Paneth-cell-rich domains establishes the site for the upward invagination that initiates fission. [Mh] Termos MeSH primário: Mucosa Intestinal/citologia Celulas de Paneth/citologia Receptores Acoplados a Proteínas-G/metabolismo Nicho de Células-Tronco Células-Tronco/citologia [Mh] Termos MeSH secundário: Fatores Etários Animais Adesão Celular Contagem de Células Divisão Celular Proliferação Celular Integrina beta4/metabolismo Mucosa Intestinal/metabolismo Intestino Delgado/citologia Intestino Delgado/metabolismo Camundongos Endogâmicos C57BL Camundongos Transgênicos Microscopia Confocal Modelos Biológicos Organoides/citologia Organoides/metabolismo Celulas de Paneth/metabolismo Receptores Acoplados a Proteínas-G/genética Células-Tronco/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Integrin beta4); 0 (Lgr5 protein, mouse); 0 (Receptors, G-Protein-Coupled) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160628 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pbio.1002491

9 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27193492 [Au] Autor: Li X; Qian H; Sogame R; Hirako Y; Tsuruta D; Ishii N; Koga H; Tsuchisaka A; Jin Z; Tsubota K; Fukumoto A; Sotozono C; Kinoshita S; Hashimoto T [Ad] Endereço: Department of Dermatology, Kurume University School of Medicine, and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka. [Ti] Título: Integrin ß4 is a major target antigen in pure ocular mucous membrane pemphigoid. [So] Source: Eur J Dermatol;26(3):247-53, 2016 Jun 01. [Is] ISSN: 1952-4013 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: Previous studies of ocular mucous membrane pemphigoid (OMMP) have identified several components of the basement membrane zone to be autoantigens, including integrin ß4. However, there are no extensive or definitive reported studies that address this, particularly in pure OMMP. To clarify the major autoantigens in pure OMMP. In this study, we examined sera from 43 pure OMMP patients for both IgG and IgA antibodies using newly developed immunoblotting analyses with a hemidesmosome-rich fraction and various recombinant proteins of integrin α6ß4, in addition to our routine immune-serological tests. Using a hemidesmosome-rich fraction, sera from patients with pure OMMP demonstrated reactivity of IgG and/or IgA antibodies to integrin ß4, BP180 and laminin-332. The reactivity of pure OMMP sera to integrin ß4 was further confirmed by immunoblotting using integrin ß4 recombinant proteins. Using concentrated supernatant of HaCaT cells, only one serum sample showed positive IgG and IgA reactivity to LAD-1, the ectodomain of BP180. None of the pure OMMP sera reacted with any autoantigens on immunoblotting using normal human epidermal or dermal extracts, or purified human laminin-332. Integrin ß4 was considered to be the major and specific autoantigen for pure OMMP. The new methods established in this study are useful for detection of various autoantigens, particularly integrin ß4. [Mh] Termos MeSH primário: Imunoglobulina A/sangue Imunoglobulina G/sangue Integrina beta4/imunologia Penfigoide Mucomembranoso Benigno/sangue [Mh] Termos MeSH secundário: Autoanticorpos/sangue Autoantígenos/imunologia Estudos de Casos e Controles Moléculas de Adesão Celular/imunologia Técnica Indireta de Fluorescência para Anticorpo Hemidesmossomos Seres Humanos Immunoblotting/métodos Colágenos não Fibrilares/imunologia Penfigoide Mucomembranoso Benigno/imunologia Proteínas Recombinantes/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Autoantibodies); 0 (Autoantigens); 0 (Cell Adhesion Molecules); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Integrin beta4); 0 (Non-Fibrillar Collagens); 0 (Recombinant Proteins); 0 (collagen type XVII); 0 (kalinin) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170623 [Lr] Data última revisão: 170623 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160520 [St] Status: MEDLINE [do] DOI: 10.1684/ejd.2016.2772

10 / 490

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27178753 [Au] Autor: Tai YL; Lai IR; Peng YJ; Ding ST; Shen TL [Ad] Endereço: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan. [Ti] Título: Activation of focal adhesion kinase through an interaction with ß4 integrin contributes to tumorigenicity of colon cancer. [So] Source: FEBS Lett;590(12):1826-37, 2016 06. [Is] ISSN: 1873-3468 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: High expression of either ß4 integrin or focal adhesion kinase (FAK) has been reported in human colon cancer. However, it remains unclear how ß4 integrin together with FAK contributes to the tumorigenicity of colon cancer. Here, we demonstrate that the co-overexpression of ß4 integrin and FAK positively correlates with advanced stages of human colon cancer. Activated ß4 integrin interacts with FAK and subsequently induces FAK phosphorylation at Tyr397. Furthermore, ablation of the ß4 integrin/FAK complex and/or FAK activation impair colon cancer cell proliferation, anchorage-independent growth, and tumorigenicity. Our data indicate that the ß4 integrin/FAK complex and subsequent FAK activation are essential regulators during the tumorigenicity of colon cancer, and we suggest an alternative strategy for colon cancer therapy. [Mh] Termos MeSH primário: Neoplasias do Colo/metabolismo Quinase 1 de Adesão Focal/metabolismo Integrina beta4/metabolismo Proteínas de Neoplasias/metabolismo [Mh] Termos MeSH secundário: Neoplasias do Colo/genética Neoplasias do Colo/patologia Feminino Quinase 1 de Adesão Focal/genética Seres Humanos Integrina beta4/genética Masculino Proteínas de Neoplasias/genética Fosforilação/genética [Pt] Tipo de publicação: LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (ITGB4 protein, human); 0 (Integrin beta4); 0 (Neoplasm Proteins); EC 2.7.10.2 (Focal Adhesion Kinase 1); EC 2.7.10.2 (PTK2 protein, human) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 171126 [Lr] Data última revisão: 171126 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160515 [St] Status: MEDLINE [do] DOI: 10.1002/1873-3468.12215

---

página 1 de 49

ir para página

Refinar a pesquisa

Base de dados : MEDLINE

Formulário avançado

		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde