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[PMID]: | 28300755 |
[Au] Autor: | Garimella R; Tadikonda P; Tawfik O; Gunewardena S; Rowe P; Van Veldhuizen P |
[Ad] Endereço: | Division of Medical Clinical Oncology, The University of Kansas Medical Center, Kansas City, KS 66160, USA. Rama.Garimella@va.gov. |
[Ti] Título: | Vitamin D Impacts the Expression of Runx2 Target Genes and Modulates Inflammation, Oxidative Stress and Membrane Vesicle Biogenesis Gene Networks in 143B Osteosarcoma Cells. |
[So] Source: | Int J Mol Sci;18(3), 2017 Mar 16. | [Is] ISSN: | 1422-0067 |
[Cp] País de publicação: | Switzerland |
[La] Idioma: | eng |
[Ab] Resumo: | Osteosarcoma (OS) is an aggressive malignancy of bone affecting children, adolescents and young adults. Understanding vitamin D metabolism and vitamin D regulated genes in OS is an important aspect of vitamin D/cancer paradigm, and in evaluating vitamin D as adjuvant therapy for human OS. Vitamin D treatment of 143B OS cells induced significant and novel changes in the expression of genes that regulate: (a) inflammation and immunity; (b) formation of reactive oxygen species, metabolism of cyclic nucleotides, sterols, vitamins and mineral (calcium), quantity of gap junctions and skeletogenesis; (c) bone mineral density; and (d) cell viability of skeletal cells, aggregation of bone cancer cells and exocytosis of secretory vesicles. Ingenuity pathway analysis revealed significant reduction in Runx2 target genes such as fibroblast growth factor -1, -12 ( and ), bone morphogenetic factor-1 ( ), SWI/SNF related, matrix associated actin dependent regulator of chromatin subfamily a, member 4 ( ), Matrix extracellular phosphoglycoprotein ( ), Integrin, ß4 ( ), Matrix Metalloproteinase -1, -28 ( and ), and signal transducer and activator of transcription-4 ( ) in vitamin D treated 143B OS cells. These genes interact with the inflammation, oxidative stress and membrane vesicle biogenesis gene networks. Vitamin D not only inhibited the expression of Runx2 target genes , and kallikrein related peptidase-7 ( ), but also migration and invasion of 143B OS cells. Vitamin D regulated Runx2 target genes or their products represent potential therapeutic targets and laboratory biomarkers for applications in translational oncology. |
[Mh] Termos MeSH primário: |
Subunidade alfa 1 de Fator de Ligação ao Core/genética Redes Reguladoras de Genes Osteossarcoma/metabolismo Estresse Oxidativo Vesículas Transportadoras/genética Vitamina D/farmacologia Vitaminas/farmacologia
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[Mh] Termos MeSH secundário: |
Proteína Morfogenética Óssea 1/genética Proteína Morfogenética Óssea 1/metabolismo Linhagem Celular Tumoral Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo DNA Helicases/genética DNA Helicases/metabolismo Proteínas da Matriz Extracelular/genética Proteínas da Matriz Extracelular/metabolismo Fatores de Crescimento de Fibroblastos/genética Fatores de Crescimento de Fibroblastos/metabolismo Glicoproteínas/genética Glicoproteínas/metabolismo Seres Humanos Inflamação/genética Integrina beta4/genética Integrina beta4/metabolismo Calicreínas/genética Calicreínas/metabolismo Metaloproteinases da Matriz/genética Metaloproteinases da Matriz/metabolismo Proteínas Nucleares/genética Proteínas Nucleares/metabolismo Fosfoproteínas/genética Fosfoproteínas/metabolismo Fatores de Transcrição/genética Fatores de Transcrição/metabolismo Vesículas Transportadoras/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Core Binding Factor Alpha 1 Subunit); 0 (Extracellular Matrix Proteins); 0 (Glycoproteins); 0 (Integrin beta4); 0 (MEPE protein, human); 0 (Nuclear Proteins); 0 (Phosphoproteins); 0 (RUNX2 protein, human); 0 (Transcription Factors); 0 (Vitamins); 1406-16-2 (Vitamin D); 62031-54-3 (Fibroblast Growth Factors); EC 3.4.21.- (KLK7 protein, human); EC 3.4.21.- (Kallikreins); EC 3.4.24.- (Matrix Metalloproteinases); EC 3.4.24.19 (BMP1 protein, human); EC 3.4.24.19 (Bone Morphogenetic Protein 1); EC 3.6.1.- (SMARCA4 protein, human); EC 3.6.4.- (DNA Helicases) |
[Em] Mês de entrada: | 1704 |
[Cu] Atualização por classe: | 170420 |
[Lr] Data última revisão:
| 170420 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170317 |
[St] Status: | MEDLINE |
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