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Pesquisa : D12.776.543.750.705.408.600 [Categoria DeCS]
Referências encontradas : 544 [refinar]
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  1 / 544 MEDLINE  
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[PMID]:26961374
[Au] Autor:Nibali L; Novoa L; Donos N; Henderson B; Blanco J; Tomas I
[Ad] Endereço:Clinical Oral Research Centre, Institute of Dentistry, Queen Mary University London (QMUL), London, UK.
[Ti] Título:Leukocyte receptor expression in chronic periodontitis.
[So] Source:Clin Oral Investig;20(9):2559-2564, 2016 Dec.
[Is] ISSN:1436-3771
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVE: Microbial recognition in the periodontium through specific leukocyte receptors gives rise to the response which in susceptible individuals can lead to periodontal diseases. The aim of this study was to explore the expression of leukocyte receptors in the gingival tissues of chronic periodontitis patients and to analyse differences between diseased and control sites (sites with probing pocket depth <4 mm). MATERIAL AND METHODS: Thirty-seven chronic periodontitis patients were included in the study. Gingival biopsies were harvested from diseased and control sites and processed by flow cytometry for the determination of the expression of 16 leukocyte receptors (CD4, CD8, CD11b, CD14, CD16, CD19, CD25, CD28, CD49d, CD49e, CD62, CD71, CD80, CCR7, Ly6G and HLA-DR). RESULTS: Expression of all studied receptors was higher in test compared with control sites (p < 0.005). Sampled sites with less bleeding on probing exhibited higher expression of CD16 and CD14 receptors (p = 0.020 and 0.011, respectively). CONCLUSIONS: This study points towards considerable differences in the expression of leukocyte receptors between diseased and control sites in the same periodontal patients.
[Mh] Termos MeSH primário: Periodontite Crônica/imunologia
Receptores de Adesão de Leucócito/imunologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Biópsia
Periodontite Crônica/microbiologia
Feminino
Citometria de Fluxo
Seres Humanos
Masculino
Meia-Idade
Índice Periodontal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160311
[St] Status:MEDLINE
[do] DOI:10.1007/s00784-016-1774-7


  2 / 544 MEDLINE  
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[PMID]:25998986
[Au] Autor:Mócsai A; Walzog B; Lowell CA
[Ad] Endereço:Department of Physiology, Semmelweis University School of Medicine, Tuzoltó utca 37-47, 1094 Budapest, Hungary MTA-SE 'Lendület' Inflammation Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, 1094 Budapest, Hungary mocsai.attila@med.semmelweis-univ.hu.
[Ti] Título:Intracellular signalling during neutrophil recruitment.
[So] Source:Cardiovasc Res;107(3):373-85, 2015 Aug 01.
[Is] ISSN:1755-3245
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recruitment of leucocytes such as neutrophils to the extravascular space is a critical step of the inflammation process and plays a major role in the development of various diseases including several cardiovascular diseases. Neutrophils themselves play a very active role in that process by sensing their environment and responding to the extracellular cues by adhesion and de-adhesion, cellular shape changes, chemotactic migration, and other effector functions of cell activation. Those responses are co-ordinated by a number of cell surface receptors and their complex intracellular signal transduction pathways. Here, we review neutrophil signal transduction processes critical for recruitment to the site of inflammation. The two key requirements for neutrophil recruitment are the establishment of appropriate chemoattractant gradients and the intrinsic ability of the cells to migrate along those gradients. We will first discuss signalling steps required for sensing extracellular chemoattractants such as chemokines and lipid mediators and the processes (e.g. PI3-kinase pathways) leading to the translation of extracellular chemoattractant gradients to polarized cellular responses. We will then discuss signal transduction by leucocyte adhesion receptors (e.g. tyrosine kinase pathways) which are critical for adhesion to, and migration through the vessel wall. Finally, additional neutrophil signalling pathways with an indirect effect on the neutrophil recruitment process, e.g. through modulation of the inflammatory environment, will be discussed. Mechanistic understanding of these pathways provide better understanding of the inflammation process and may point to novel therapeutic strategies for controlling excessive inflammation during infection or tissue damage.
[Mh] Termos MeSH primário: Infiltração de Neutrófilos
Transdução de Sinais
Migração Transendotelial e Transepitelial
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Receptores de Formil Peptídeo/metabolismo
Receptores de Adesão de Leucócito/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Receptors, Formyl Peptide); 0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150523
[St] Status:MEDLINE
[do] DOI:10.1093/cvr/cvv159


  3 / 544 MEDLINE  
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[PMID]:18711433
[Au] Autor:Friedl P; Weigelin B
[Ad] Endereço:Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Geert Grooteplein 28, 6525GA Nijmegen, 6500 HB Nijmegen, The Netherlands. p.friedl@ncmls.ru.nl
[Ti] Título:Interstitial leukocyte migration and immune function.
[So] Source:Nat Immunol;9(9):960-9, 2008 Sep.
[Is] ISSN:1529-2916
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The trafficking of leukocytes into and within lymphoid and peripheral tissues is central to immune cell development, immunosurveillance and effector function. Interstitial leukocyte trafficking is the result of amoeboid polarization and migration, guided by soluble or tissue-bound chemoattractant signals for positioning and local arrest. In contrast to other migration modes, amoeboid movement is particularly suited for scanning cellular networks and tissues. Here, we review mechanisms of leukocyte migration and sensing involved in diapedesis, tissue-based interstitial migration and egress, immune cell positioning in inflammation, and emerging therapeutic interference strategies.
[Mh] Termos MeSH primário: Movimento Celular/imunologia
Quimiotaxia de Leucócito
Sistema Imunitário/citologia
Inflamação/imunologia
Leucócitos/imunologia
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Sistema Imunitário/fisiologia
Inflamação/patologia
Receptores de Adesão de Leucócito/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:0810
[Cu] Atualização por classe:080819
[Lr] Data última revisão:
080819
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080820
[St] Status:MEDLINE
[do] DOI:10.1038/ni.f.212


  4 / 544 MEDLINE  
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[PMID]:18711430
[Ti] Título:On the move.
[So] Source:Nat Immunol;9(9):947, 2008 Sep.
[Is] ISSN:1529-2916
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Movimento Celular
Leucócitos/fisiologia
[Mh] Termos MeSH secundário: Animais
Adesão Celular
Quimiotaxia de Leucócito
Seres Humanos
Receptores de Adesão de Leucócito/fisiologia
Transdução de Sinais
[Pt] Tipo de publicação:EDITORIAL; INTRODUCTORY JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:0810
[Cu] Atualização por classe:080819
[Lr] Data última revisão:
080819
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080820
[St] Status:MEDLINE
[do] DOI:10.1038/ni0908-947


  5 / 544 MEDLINE  
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[PMID]:18689665
[Au] Autor:Dassanayake RP; Liu W; Davis WC; Foreyt WJ; Srikumaran S
[Ad] Endereço:Department of Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.
[Ti] Título:Bighorn sheep beta2-integrin LFA-1 serves as a receptor for Mannheimia haemolytica leukotoxin.
[So] Source:J Wildl Dis;44(3):743-7, 2008 Jul.
[Is] ISSN:0090-3558
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mannheimia haemolytica is an important cause of pneumonia in bighorn sheep (BHS; Ovis canadensis). Leukotoxin (Lkt), the primary virulence determinant of M. haemolytica, induces cytolysis of all subsets of leukocytes. Previously, we have shown that CD18, the beta subunit of beta2-integrins, mediates Lkt-induced cytolysis. However, it is not clear whether CD18 of all three beta2-integrins, LFA-1, Mac-1, and CR4, mediates Lkt-induced cytolysis. The objective of this study was to determine whether BHS LFA-1 (CD11a/CD18) serves as a receptor for Lkt. Plasmids encoding cDNA for BHS CD11a and CD18 were cotransfected into Lkt-resistant HEK-293 cells. Flow cytometric analysis of transfectants confirmed cell surface expression of BHS LFA- 1, Lkt-LFA-1 binding and Lkt-induced intra-cellular calcium elevation. More importantly, the transfectants were efficiently lysed by Lkt in a concentration-dependent manner. Collectively, these results indicate that BHS LFA-1 serves as a functional receptor for M. haemolytica Lkt.
[Mh] Termos MeSH primário: Antígenos CD18/imunologia
Exotoxinas/biossíntese
Mannheimia haemolytica/metabolismo
Pasteurelose Pneumônica/imunologia
Doenças dos Ovinos/imunologia
Carneiro da Montanha
[Mh] Termos MeSH secundário: Animais
Exotoxinas/metabolismo
Citometria de Fluxo/veterinária
Leucócitos/metabolismo
Mannheimia haemolytica/imunologia
Pasteurelose Pneumônica/microbiologia
Receptores de Adesão de Leucócito/metabolismo
Ovinos
Doenças dos Ovinos/microbiologia
Transfecção/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CD18 Antigens); 0 (Exotoxins); 0 (Receptors, Leukocyte-Adhesion); 0 (leukotoxin)
[Em] Mês de entrada:0811
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080812
[St] Status:MEDLINE


  6 / 544 MEDLINE  
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[PMID]:18647111
[Au] Autor:Thomas W
[Ad] Endereço:Department of Bioengineering, University of Washington, Seattle, WA 98195-5061, USA. wendyt@u.washington.edu
[Ti] Título:Catch bonds in adhesion.
[So] Source:Annu Rev Biomed Eng;10:39-57, 2008.
[Is] ISSN:1523-9829
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One of the most exciting discoveries in biological adhesion is the recent and counter-intuitive observation that the lifetimes of some biological adhesive bonds, called catch bonds, are enhanced by tensile mechanical force. At least two types of adhesive proteins have been shown to form catch bonds--blood proteins called selectins and a bacterial protein called FimH. Both mediate shear-enhanced adhesion, in which cells bind more strongly at high shear than at low shear. Single-molecule experiments and cell-free assays have now clearly demonstrated that catch bonds exist and mediate shear-enhanced adhesion. However, the mechanics of cellular organelles also contribute to shear-enhanced adhesion by modulating the force applied to catch bonds. This review examines how individual catch bond behavior contributes to shear-enhanced cellular adhesion for the two best-understood examples. The lessons from these systems offer design principles for understanding other types of shear-enhanced adhesion and for engineering nanostructured force-dependent adhesives out of catch bonds.
[Mh] Termos MeSH primário: Aderência Bacteriana/fisiologia
Moléculas de Adesão Celular/fisiologia
Adesão Celular/fisiologia
Leucócitos/fisiologia
Mecanotransdução Celular/fisiologia
Receptores de Adesão de Leucócito/fisiologia
[Mh] Termos MeSH secundário: Sítios de Ligação
Moléculas de Adesão Celular/química
Ligação Proteica
Receptores de Adesão de Leucócito/química
Resistência ao Cisalhamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cell Adhesion Molecules); 0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:0810
[Cu] Atualização por classe:080723
[Lr] Data última revisão:
080723
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080724
[St] Status:MEDLINE
[do] DOI:10.1146/annurev.bioeng.10.061807.160427


  7 / 544 MEDLINE  
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[PMID]:18267122
[Au] Autor:Chiu PL; Ng BH; Chang GW; Gordon S; Lin HH
[Ad] Endereço:Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.
[Ti] Título:Putative alternative trans-splicing of leukocyte adhesion-GPCR pre-mRNAs generates functional chimeric receptors.
[So] Source:FEBS Lett;582(5):792-8, 2008 Mar 05.
[Is] ISSN:0014-5793
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The EGF-TM7 receptors, a subfamily of adhesion-GPCRs mostly restricted to leukocytes, are known to express multiple functional protein isoforms through extensive alternative cis-splicing. Here, we demonstrate that EGF-TM7 pre-mRNAs also undergo the rare trans-splicing, leading to the generation of functional chimeric receptors. RT-PCR and in silico analyses of EMR2 transcripts identified unique fragments containing the EGF-like motif 3 of a closely related EGF-TM7 gene, CD97, in addition to the alternative cis-spliced products. The sequence swapping is restricted to the EGF-3 exon, generating unique EMR2(1-2-3*-5) and EMR2(1-2-3*-4-5) molecules, which are functional in ligand-binding as the wild-type EMR2(1-2-3-4-5) and CD97(1-2-3-4-5) receptors. Our results suggest that human leukocytes employ trans-splicing as well as cis-splicing to increase the repertoire of functional adhesion-GPCRs.
[Mh] Termos MeSH primário: Processamento Alternativo/genética
Antígenos CD/genética
Glicoproteínas de Membrana/genética
Precursores de RNA/genética
Receptores Acoplados a Proteínas-G/genética
Receptores de Adesão de Leucócito/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Linhagem Celular
Células Clonais
Biologia Computacional
Éxons/genética
Etiquetas de Sequências Expressas
Seres Humanos
Íntrons/genética
Ligantes
Dados de Sequência Molecular
Isoformas de Proteínas/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CD97 protein, human); 0 (EMR2 protein, human); 0 (Ligands); 0 (Membrane Glycoproteins); 0 (Protein Isoforms); 0 (RNA Precursors); 0 (RNA, Messenger); 0 (Receptors, G-Protein-Coupled); 0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:0804
[Cu] Atualização por classe:080226
[Lr] Data última revisão:
080226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080213
[St] Status:MEDLINE
[do] DOI:10.1016/j.febslet.2008.02.004


  8 / 544 MEDLINE  
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[PMID]:17760416
[Au] Autor:Jayagopal A; Russ PK; Haselton FR
[Ad] Endereço:Department of Biomedical Engineering, Vanderbilt University, USA.
[Ti] Título:Surface engineering of quantum dots for in vivo vascular imaging.
[So] Source:Bioconjug Chem;18(5):1424-33, 2007 Sep-Oct.
[Is] ISSN:1043-1802
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quantum dot-antibody bioconjugates (QD-mAb) were synthesized incorporating PEG cross-linkers and Fc-shielding mAb fragments to increase in vivo circulation times and targeting efficiency. Microscopy of endothelial cell cultures incubated with QD-mAb directed against cell adhesion molecules (CAMs), when shielded to reduce Fc-mediated interactions, were more specific for their molecular targets. In vitro flow cytometry indicated that surface engineered QD-mAb labeled leukocyte subsets with minimal Fc-mediated binding. Nontargeted QD-mAb nanoparticles with Fc-blockade featured 64% (endothelial cells) and 53% (leukocytes) lower nonspecific binding than non-Fc-blocked nanoparticles. Spectrally distinct QD-mAb targeted to the cell adhesion molecules (CAMs) PECAM-1, ICAM-1, and VCAM-1 on the retinal endothelium in a rat model of diabetes were imaged in vivo using fluorescence angiography. Endogenously labeled circulating and adherent leukocyte subsets were imaged in rat models of diabetes and uveitis using QD-mAb targeted to RP-1 and CD45. Diabetic rats exhibited increased fluorescence in the retinal vasculature from QD bioconjugates to ICAM-1 and VCAM-1 but not PECAM-1. Both animal models exhibited leukocyte rolling and leukostasis in capillaries. Examination of retinal whole mounts prepared after in vivo imaging confirmed the fluorescence patterns seen in vivo. Comparison of the timecourse of retinal fluorescence from Fc-shielded and non-Fc-shielded bioconjugates indicated nonspecific uptake and increased clearance of the non-Fc-shielded QD-mAb. This combination of QD surface design elements offers a promising new in vivo approach to specifically label vascular cells and biomolecules of interest.
[Mh] Termos MeSH primário: Anticorpos Monoclonais
Diabetes Mellitus Experimental/patologia
Endotélio Vascular/patologia
Imunofluorescência/métodos
Pontos Quânticos
[Mh] Termos MeSH secundário: Animais
Adesão Celular/fisiologia
Técnicas de Cultura de Células
Diabetes Mellitus Experimental/metabolismo
Endotélio Vascular/metabolismo
Citometria de Fluxo
Microscopia de Fluorescência
Ratos
Receptores de Adesão de Leucócito/metabolismo
Vasos Retinianos/metabolismo
Vasos Retinianos/patologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Receptors, Leukocyte-Adhesion)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:161122
[Lr] Data última revisão:
161122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070901
[St] Status:MEDLINE


  9 / 544 MEDLINE  
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[PMID]:17408504
[Au] Autor:Tang J; Ley KF; Hunt CA
[Ad] Endereço:The UCSF/UCB Joint Graduate Group in Bioengineering, University of California, Berkeley, CA, USA. jont@berkeley.edu
[Ti] Título:Dynamics of in silico leukocyte rolling, activation, and adhesion.
[So] Source:BMC Syst Biol;1:14, 2007 Feb 19.
[Is] ISSN:1752-0509
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We present a multilevel, agent based, in silico model that represents the dynamics of rolling, activation, and adhesion of individual leukocytes in vitro. Object-oriented software components were designed, verified, plugged together, and then operated in ways that represent the molecular and cellular mechanisms believed responsible for leukocyte rolling and adhesion. The result is an in silico analogue of an experimental in vitro system. The experimentally measured, phenotypic attributes of the analogue were compared and contrasted to those of leukocytes in vitro from three different experimental conditions. RESULTS: The individual in silico dynamics of "rolling" on simulated P-selectin, and separately on simulated VCAM-1, were an acceptable match to individual in vitro distance-time and velocity-time measurements. The analogues are also able to represent the transition from rolling to adhesion on P-selectin and VCAM-1 in the presence of GRO-alpha chemokine. The individual in silico and in vitro behavioral similarities translated successfully to population level measures. These behavioral similarities were enabled in part by subdividing the functionality of the analogue's surface into 600 independent, "cell"-controlled, equally capable modules of comparable functionality. CONCLUSION: The overlap in phenotypic attributes of our analogue with those of leukocytes in vitro confirm the considerable potential of our model for studying the key events that determine the behavioral outcome of individual leukocytes during rolling, activation, and adhesion. Our results provide an important foundation and framework for future in silico research into plausible causal links between well-documented, subcellular molecular level events and the variety of systemic phenotypic attributes that distinguish normal leukocyte adhesion from abnormal disease-associated adhesion.
[Mh] Termos MeSH primário: Simulação por Computador
Migração e Rolagem de Leucócitos
Ativação Linfocitária
Modelos Imunológicos
[Mh] Termos MeSH secundário: Adesão Celular
Quimiocina CXCL1/imunologia
Seres Humanos
Neutrófilos/imunologia
Selectina-P/imunologia
Receptores de Adesão de Leucócito/imunologia
Molécula 1 de Adesão de Célula Vascular/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemokine CXCL1); 0 (P-Selectin); 0 (Receptors, Leukocyte-Adhesion); 0 (Vascular Cell Adhesion Molecule-1)
[Em] Mês de entrada:0707
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070406
[St] Status:MEDLINE


  10 / 544 MEDLINE  
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[PMID]:17347395
[Au] Autor:Liu W; Brayton KA; Davis WC; Mansfield K; Lagerquist J; Foreyt W; Srikumaran S
[Ad] Endereço:Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040, USA.
[Ti] Título:Mannheimia (Pasteurella) haemolytica leukotoxin utilizes CD18 as its receptor on bighorn sheep leukocytes.
[So] Source:J Wildl Dis;43(1):75-81, 2007 Jan.
[Is] ISSN:0090-3558
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pneumonia caused by Mannheimia (Pasteurella) haemolytica is a highly fatal disease of bighorn sheep (Ovis canadensis). Leukotoxin (Lkt), secreted by M. haemolytica, is an important virulence factor of this organism, and is cytolytic to bighorn sheep leukocytes. Previously, we have shown that CD18, the beta subunit of beta2 integrins, serves as the receptor for Lkt on bovine leukocytes. Furthermore, anti-CD18 antibodies inhibit Lkt-induced cytotoxicity of bighorn sheep leukocytes. Therefore, we hypothesized that Lkt utilizes CD18 as its receptor on bighorn sheep leukocytes. Confirmation of bighorn sheep CD18 as a receptor for Lkt requires the demonstration that the recombinant expression of bighorn sheep CD18 in Lkt-nonsusceptible cells renders them susceptible to Lkt. Therefore, we transfected cDNA encoding CD18 of bighorn sheep into a Lkt-nonsusceptible murine cell line. Cell surface expression of bighorn sheep CD18 on the transfectants was tested by flow cytometry with anti-CD18 antibodies. Transfectants stably expressing bighorn sheep CD18 on their surface were subjected to flow cytometric analysis for detection of Lkt binding, and cytotoxicity assays for detection of Lkt-induced cytotoxicity. Leukotoxin bound to the transfectants. More importantly, the transfectants were effectively lysed by Lkt in a concentration-dependent manner, whereas the parent cells were not. These results clearly indicate that M. haemolytica Lkt utilizes CD18 as a receptor on bighorn sheep leukocytes. Identification of CD18 as a receptor for Lkt on bighorn sheep leukocytes should enhance our understanding of the pathogenesis of pneumonia, which in turn should help in the development of control measures against this fatal disease of bighorn sheep.
[Mh] Termos MeSH primário: Antígenos CD18/imunologia
Exotoxinas/biossíntese
Mannheimia haemolytica/metabolismo
Pasteurelose Pneumônica/microbiologia
Doenças dos Ovinos/microbiologia
Carneiro da Montanha
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Citometria de Fluxo/veterinária
Camundongos
Pasteurelose Pneumônica/imunologia
Receptores de Adesão de Leucócito/metabolismo
Doenças dos Ovinos/imunologia
Transfecção/veterinária
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CD18 Antigens); 0 (Exotoxins); 0 (Receptors, Leukocyte-Adhesion); 0 (leukotoxin)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070310
[St] Status:MEDLINE



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde