Base de dados : MEDLINE
Pesquisa : D12.776.543.750.705.852.760.097 [Categoria DeCS]
Referências encontradas : 5460 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 546 ir para página                         

  1 / 5460 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28746783
[Au] Autor:D'Souza L; Gupta SL; Bal V; Rath S; George A
[Ad] Endereço:National Institute of Immunology, New Delhi, India.
[Ti] Título:CD73 expression identifies a subset of IgM antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent.
[So] Source:Immunology;152(4):602-612, 2017 12.
[Is] ISSN:1365-2567
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:B-cell memory was long characterized as isotype-switched, somatically mutated and germinal centre (GC)-derived. However, it is now clear that the memory pool is a complex mixture that includes unswitched and unmutated cells. Further, expression of CD73, CD80 and CD273 has allowed the categorization of B-cell memory into multiple subsets, with combinatorial expression of the markers increasing with GC progression, isotype-switching and acquisition of somatic mutations. We have extended these findings to determine whether these markers can be used to identify IgM memory phenotypically as arising from T-dependent versus T-independent responses. We report that CD73 expression identifies a subset of antigen-experienced IgM cells that share attributes of functional B-cell memory. This subset is reduced in the spleens of T-cell-deficient and CD40-deficient mice and in mixed marrow chimeras made with mutant and wild-type marrow, the proportion of CD73 IgM memory is restored in the T-cell-deficient donor compartment but not in the CD40-deficient donor compartment, indicating that CD40 ligation is involved in its generation. We also report that CD40 signalling supports optimal expression of CD73 on splenic T cells and age-associated B cells (ABCs), but not on other immune cells such as neutrophils, marginal zone B cells, peritoneal cavity B-1 B cells and regulatory T and B cells. Our data indicate that in addition to promoting GC-associated memory generation during B-cell differentiation, CD40-signalling can influence the composition of the unswitched memory B-cell pool. They also raise the possibility that a fraction of ABCs may represent T-cell-dependent IgM memory.
[Mh] Termos MeSH primário: 5´-Nucleotidase/imunologia
Antígenos CD40/imunologia
Regulação da Expressão Gênica/imunologia
Imunoglobulina M/imunologia
Memória Imunológica
Transdução de Sinais/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: 5'-Nucleotidase/genética
Animais
Linfócitos B/imunologia
Antígenos CD40/genética
Imunoglobulina M/genética
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Neutrófilos/imunologia
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CD40 Antigens); 0 (Immunoglobulin M); EC 3.1.3.5 (5'-Nucleotidase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/imm.12800


  2 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28461109
[Au] Autor:Amrouche K; Jamin C
[Ad] Endereço:UMR 1227, Lymphocytes B et Autoimmunité, Université de Brest, INSERM, Brest, France; LabEx IGO "Immunotherapy, Graft, Oncology", Brest, France.
[Ti] Título:Influence of drug molecules on regulatory B cells.
[So] Source:Clin Immunol;184:1-10, 2017 11.
[Is] ISSN:1521-7035
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:By their suppressive functions, regulatory B (Breg) cells are considered as key elements in the control and development of various disease states. Many signals can induce Bregs in vivo and in vitro and often from heterogeneous populations. Several specific signals delivered in a timely immunological context contribute to the establishment of Bregs. These are endogenous and physiological signals or stimuli, widely discussed in the literature participating in the establishment of an effective immune response. However, exogenous signals, much less clearly identified can also be considered as Bregs inducers. These extrinsic signals are capable of directly or indirectly influencing the suppressive capacity of Bregs, but also their expansion and functional restoration in its absence. Faced with the excitement generated by the development of processes favoring the expansion of Bregs in mice for therapeutic purposes, the challenge today is to extrapolate such approaches in humans. This perspective may already be in effect.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Linfócitos B Reguladores/efeitos dos fármacos
Imunossupressores/farmacologia
Vitaminas/farmacologia
[Mh] Termos MeSH secundário: Corticosteroides/farmacologia
Animais
Anticorpos Monoclonais Humanizados/farmacologia
Fator Ativador de Células B/imunologia
Linfócitos B Reguladores/imunologia
Antígenos CD40/imunologia
Citocinas/imunologia
Seres Humanos
Metotrexato/farmacologia
Ácido Micofenólico/farmacologia
Pirróis/farmacologia
Quinazolinas/farmacologia
Receptores de Antígenos de Linfócitos B/imunologia
Semaforinas/farmacologia
Sirolimo/farmacologia
Receptores Toll-Like/imunologia
Tretinoína/farmacologia
Vitamina D/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenal Cortex Hormones); 0 (Antibodies, Monoclonal, Humanized); 0 (Antineoplastic Agents); 0 (B-Cell Activating Factor); 0 (CD40 Antigens); 0 (Cytokines); 0 (Immunosuppressive Agents); 0 (Pyrroles); 0 (Quinazolines); 0 (Receptors, Antigen, B-Cell); 0 (Semaphorins); 0 (Toll-Like Receptors); 0 (Vitamins); 1406-16-2 (Vitamin D); 5688UTC01R (Tretinoin); 7I279E1NZ8 (sotrastaurin); HU9DX48N0T (Mycophenolic Acid); I031V2H011 (tocilizumab); W36ZG6FT64 (Sirolimus); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  3 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28455435
[Au] Autor:Guo Y; Walsh AM; Fearon U; Smith MD; Wechalekar MD; Yin X; Cole S; Orr C; McGarry T; Canavan M; Kelly S; Lin TA; Liu X; Proudman SM; Veale DJ; Pitzalis C; Nagpal S
[Ad] Endereço:Immunology, Janssen Research, Spring House, PA 19477; yguo49@its.jnj.com snagpal2@its.jnj.com.
[Ti] Título:CD40L-Dependent Pathway Is Active at Various Stages of Rheumatoid Arthritis Disease Progression.
[So] Source:J Immunol;198(11):4490-4501, 2017 06 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The inflammatory CD40-CD40L pathway is implicated in various autoimmune diseases, but the activity status of this pathway in various stages of rheumatoid arthritis (RA) progression is unknown. In this study, we used gene signatures of CD40L stimulation derived from human immature dendritic cells and naive B cells to assess the expression of CD40-downstream genes in synovial tissues from anti-citrullinated protein Ab-positive arthralgia, undifferentiated arthritis (UA), early RA, and established RA cohorts in comparison with healthy donors. Interestingly, the expression of and active full-length was increased in the disease tissues, whereas that of a dominant-negative isoform was decreased. Gene set variation analysis revealed that CD40L-responsive genes in immature dendritic cells and naive B cells were significantly enriched in synovial tissues from UA, early RA, and established RA patients. Additionally, CD40L-induced naive B cell genes were also significantly enriched in synovial tissues from arthralgia patients. In our efforts to characterize downstream mediators of CD40L signaling, we have identified and as novel components of the pathway. In conclusion, our data suggest that therapeutic CD40-CD40L blocking agents may prove efficacious not only in early and established RA, but also in inhibiting the progression of the disease from arthralgia or UA to RA.
[Mh] Termos MeSH primário: Artrite Reumatoide/imunologia
Artrite/imunologia
Ligante de CD40/imunologia
Ligante de CD40/metabolismo
Progressão da Doença
Transdução de Sinais
[Mh] Termos MeSH secundário: Adulto
Idoso
Artralgia/imunologia
Artralgia/fisiopatologia
Artrite Reumatoide/fisiopatologia
Linfócitos B/efeitos dos fármacos
Linfócitos B/imunologia
Biópsia
Linfócitos T CD4-Positivos/imunologia
Antígenos CD40/deficiência
Antígenos CD40/genética
Antígenos CD40/imunologia
Antígenos CD40/metabolismo
Ligante de CD40/genética
Ligante de CD40/farmacologia
Células Dendríticas/imunologia
Células Dendríticas/fisiologia
Feminino
Voluntários Saudáveis
Seres Humanos
Ativação Linfocitária
Masculino
Meia-Idade
Líquido Sinovial/citologia
Líquido Sinovial/imunologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD40 Antigens); 147205-72-9 (CD40 Ligand)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180127
[Lr] Data última revisão:
180127
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601988


  4 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28462919
[Au] Autor:Slinger E; Thijssen R; Kater AP; Eldering E
[Ad] Endereço:Cancer Center Amsterdam, Department of Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands.
[Ti] Título:Targeting antigen-independent proliferation in chronic lymphocytic leukemia through differential kinase inhibition.
[So] Source:Leukemia;31(12):2601-2607, 2017 Dec.
[Is] ISSN:1476-5551
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The clinical success of B-cell receptor (BCR) signaling pathway inhibitors in chronic lymphocytic leukemia (CLL) is attributed to inhibition of adhesion in and migration towards the lymph node. Proliferation of CLL cells is restricted to this protective niche, but the underlying mechanism(s) is/are not known. Treatment with BCR pathway inhibitors results in rapid reductions of total clone size, while CLL cell survival is not affected, which points towards inhibition of proliferation. In vitro, BCR stimulation does not induce proliferation of CLL, but triggering via Toll-like receptor, tumor necrosis factor or cytokine receptors does. Here, we investigated the effects of clinically applied inhibitors that target BCR signaling, in the context of proliferation triggered either via CD40L/IL-21 or after CpG stimulation. CD40L/IL-21-induced proliferation could be inhibited by idelalisib and ibrutinib. We demonstrate this was due to blockade of CD40L-induced ERK-signaling. Targeting JAKs, but not SYK, blocked CD40L/IL-21-induced proliferation. In contrast, PI3K, BTK as well as SYK inhibition prevented CpG-induced proliferation. Knockdown experiments showed that CD40L/IL-21 did not co-opt upstream BCR components such as CD79A, in contrast to CpG-induced proliferation. Our data indicate that currently applied BTK/PI3K inhibitors target antigen-independent proliferation in CLL, and suggest that targeting of JAK and/or SYK might be clinically useful.
[Mh] Termos MeSH primário: Antígenos de Neoplasias/imunologia
Leucemia Linfocítica Crônica de Células B/imunologia
Leucemia Linfocítica Crônica de Células B/metabolismo
Fosfotransferases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Biomarcadores
Antígenos CD40/imunologia
Antígenos CD40/metabolismo
Linhagem Celular Tumoral
Proliferação Celular
Seres Humanos
Interleucinas/metabolismo
Janus Quinases/metabolismo
Leucemia Linfocítica Crônica de Células B/genética
NF-kappa B/metabolismo
Fosfotransferases/metabolismo
Inibidores de Proteínas Quinases/farmacologia
Receptores de Antígenos de Linfócitos B/metabolismo
Fatores de Transcrição STAT/metabolismo
Transdução de Sinais/efeitos dos fármacos
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (Biomarkers); 0 (CD40 Antigens); 0 (Interleukins); 0 (NF-kappa B); 0 (Protein Kinase Inhibitors); 0 (Receptors, Antigen, B-Cell); 0 (STAT Transcription Factors); 0 (interleukin-21); EC 2.7.- (Phosphotransferases); EC 2.7.10.2 (Janus Kinases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1038/leu.2017.129


  5 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29023539
[Au] Autor:Gardell JL; Parker DC
[Ad] Endereço:Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon, United States of America.
[Ti] Título:Despite disorganized synapse structure, Th2 cells maintain directional delivery of CD40L to antigen-presenting B cells.
[So] Source:PLoS One;12(10):e0186573, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Upon recognition of peptide displayed on MHC molecules, Th1 and Th2 cells form distinct immunological synapse structures. Th1 cells have a bull's eye synapse structure with TCR/ MHC-peptide interactions occurring central to a ring of adhesion molecules, while Th2 cells have a multifocal synapse with small clusters of TCR/MHC interactions throughout the area of T cell/antigen-presenting cell interaction. In this study, we investigated whether this structural difference in the immunological synapse affects delivery of T cell help. The immunological synapse is thought to ensure antigen-specific delivery of cytolytic granules and killing of target cells by NK cells and cytolytic T cells. In helper T cells, it has been proposed that the immunological synapse may direct delivery of other effector molecules including cytokines. CD40 ligand (CD40L) is a membrane-bound cytokine essential for antigen-specific T cell help for B cells in the antibody response. We incubated Th1 and Th2 cells overnight with a mixture of antigen-presenting and bystander B cells, and the delivery of CD40L to B cells and subsequent B cell responses were compared. Despite distinct immunological synapse structures, Th1 and Th2 cell do not differ in their ability to deliver CD40L and T cell help in an antigen-specific fashion, or in their susceptibility to inhibition of help by a blocking anti-CD40L antibody.
[Mh] Termos MeSH primário: Células Apresentadoras de Antígenos/metabolismo
Linfócitos B/metabolismo
Ligante de CD40/metabolismo
Sinapses/química
Células Th2/metabolismo
[Mh] Termos MeSH secundário: Animais
Anticorpos/imunologia
Anticorpos/farmacologia
Células Apresentadoras de Antígenos/citologia
Linfócitos B/imunologia
Antígenos CD40/deficiência
Antígenos CD40/genética
Ligante de CD40/genética
Ligante de CD40/imunologia
Ciclosporina/farmacologia
Feminino
Sinapses Imunológicas/química
Sinapses Imunológicas/efeitos dos fármacos
Sinapses Imunológicas/imunologia
Interleucina-4/imunologia
Ativação Linfocitária/efeitos dos fármacos
Masculino
Camundongos
Camundongos Knockout
Camundongos Transgênicos
Microscopia Confocal
Sinapses/metabolismo
Células Th1/citologia
Células Th1/metabolismo
Células Th2/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (CD40 Antigens); 147205-72-9 (CD40 Ligand); 207137-56-2 (Interleukin-4); 83HN0GTJ6D (Cyclosporine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186573


  6 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28899907
[Au] Autor:Zhao Q; Kim T; Pang J; Sun W; Yang X; Wang J; Song Y; Zhang H; Sun H; Rangan V; Deshpande S; Tang H; Cvijic ME; Westhouse R; Olah T; Xie J; Struthers M; Salter-Cid L
[Ad] Endereço:Discovery Biology, Immuno-Science, Bristol Myers Squibb, Princeton, New Jersey, USA; qihong.zhao@bms.com.
[Ti] Título:A novel function of CXCL10 in mediating monocyte production of proinflammatory cytokines.
[So] Source:J Leukoc Biol;102(5):1271-1280, 2017 Nov.
[Is] ISSN:1938-3673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IFN-γ-inducible protein 10 (CXCL10), a chemokine that is abundantly secreted in response to inflammatory stimuli, has been implicated in the pathogenesis of multiple inflammatory diseases, such as inflammatory bowel disease. Whereas CXCL10 is traditionally recognized for recruiting pathogenic T cells to inflamed sites, its nonchemotactic role during inflammation remains poorly defined. In this report, we identified a novel function of CXCL10 in the regulation of the inflammatory potential of human monocytes to produce cytokines. We found that CXCL10 was necessary and sufficient for IFN-γ-primed human monocytes to induce a robust production of proinflammatory cytokines, such as IL-12 and IL-23. CXCL10-induced monocyte production of these cytokines depended on CXCR3 receptor engagement as well as on the Iκ B kinase and p38 MAPK signaling pathways. By using an innate-mediated murine colitis model, we demonstrated that anti-CXCL10 Ab treatment robustly suppressed the local production of myeloid-derived inflammatory cytokines and intestinal tissue damage. Together, our data unravel a previously unappreciated role of CXCL10 in the amplification of myeloid cell-mediated inflammatory responses. Targeting CXCL10 is therefore an attractive approach to treating inflammatory diseases that are driven by innate and adaptive immunity.
[Mh] Termos MeSH primário: Imunidade Adaptativa
Quimiocina CXCL10/imunologia
Colite Ulcerativa/imunologia
Doença de Crohn/imunologia
Imunidade Inata
Monócitos/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/administração & dosagem
Antígenos CD40/antagonistas & inibidores
Quimiocina CXCL10/antagonistas & inibidores
Quimiocina CXCL10/genética
Colite/induzido quimicamente
Colite/genética
Colite/imunologia
Colite/patologia
Colite Ulcerativa/genética
Colite Ulcerativa/patologia
Doença de Crohn/genética
Doença de Crohn/patologia
Feminino
Regulação da Expressão Gênica
Seres Humanos
Quinase I-kappa B/genética
Quinase I-kappa B/imunologia
Interferon gama/genética
Interferon gama/imunologia
Interleucina-12/genética
Interleucina-12/imunologia
Interleucina-23/genética
Interleucina-23/imunologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Monócitos/citologia
Cultura Primária de Células
Receptores CXCR3/genética
Receptores CXCR3/imunologia
Transdução de Sinais
Proteínas Quinases p38 Ativadas por Mitógeno/genética
Proteínas Quinases p38 Ativadas por Mitógeno/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (CD40 Antigens); 0 (Chemokine CXCL10); 0 (Cxcl10 protein, mouse); 0 (Cxcr3 protein, mouse); 0 (Interleukin-23); 0 (Receptors, CXCR3); 187348-17-0 (Interleukin-12); 82115-62-6 (Interferon-gamma); EC 2.7.11.10 (I-kappa B Kinase); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1189/jlb.5A0717-302


  7 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28796044
[Au] Autor:Chen J; Li JH; Zhao SJ; Wang DY; Zhang WZ; Liang WJ
[Ad] Endereço:aDepartment of Cardiovascular Medicine, Guangzhou Panyu Central Hospital, bPanyu District Cardiovascular Disease Research Institute of Guangzhou, Guangzhou, P.R. China.
[Ti] Título:Clinical significance of costimulatory molecules CD40/CD40L and CD134/CD134L in coronary heart disease: A case-control study.
[So] Source:Medicine (Baltimore);96(32):e7634, 2017 Aug.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to evaluate the potential role of CD40/CD40 ligand (CD40L) and CD134/CD134 ligand (CD134L) in the development of coronary heart disease (CHD) via the performance of a case-control study.The research objects were 234 cases of CHD patients and 120 cases of well-matched normal controls. Following the separation of peripheral blood mononuclear cells (PBMCs), real-time quantitative PCR (qRT-PCR), Western blot, immunohistochemistry, and flow cytometry were applied for the detection of mRNA levels and expression levels of CD40/CD40L and CD134/CD134L; meanwhile, intercellular adhesion molecule-1 (ICAM-1) and Fas protein mRNA levels were detected using qRT-PCR.There was no statistical difference in the comparison of baseline characteristics between groups, indicating comparability between groups. qRT-PCR and Western blot analysis indicated that CD40/CD40L and CD134/CD134L mRNA and protein expression levels were all increased in the CHD group than those in the control group. Flow cytometry further confirmed the similar tendency. Meanwhile, ICAM-1 and Fas protein mRNA levels were elevated in the CHD group and positively correlated with the above parameters. Furthermore, CD40/CD40L expression rates were negatively correlated with gender and different types of CHD. Meanwhile, CD134/CD134L expressions were also higher in male patients, in patients with family history, previous history of hypertension, diabetes, and cerebrovascular diseases.CD40/CD40L and CD134/CD134L are increased and may have potential correlation with clinical pathological features of patients with CHD. Further in-depth exploration of costimulatory molecules for CHD guidance as well as intrinsic mechanisms are needed combined with in vivo and in vitro experiments.
[Mh] Termos MeSH primário: Antígenos CD40/biossíntese
Ligante de CD40/biossíntese
Doença das Coronárias/fisiopatologia
Ligante OX40/biossíntese
Receptores OX40/biossíntese
[Mh] Termos MeSH secundário: Adulto
Idoso
Estudos de Casos e Controles
Feminino
Citometria de Fluxo
Seres Humanos
Molécula 1 de Adesão Intercelular/biossíntese
Masculino
Meia-Idade
RNA Mensageiro
Reação em Cadeia da Polimerase em Tempo Real
Receptor fas/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (CD40 Antigens); 0 (FAS protein, human); 0 (OX40 Ligand); 0 (RNA, Messenger); 0 (Receptors, OX40); 0 (fas Receptor); 126547-89-5 (Intercellular Adhesion Molecule-1); 147205-72-9 (CD40 Ligand)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007634


  8 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28768709
[Au] Autor:Watanabe M; Fujihara C; Radtke AJ; Chiang YJ; Bhatia S; Germain RN; Hodes RJ
[Ad] Endereço:Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD.
[Ti] Título:Co-stimulatory function in primary germinal center responses: CD40 and B7 are required on distinct antigen-presenting cells.
[So] Source:J Exp Med;214(9):2795-2810, 2017 Sep 04.
[Is] ISSN:1540-9538
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:T cell-dependent germinal center (GC) responses require coordinated interactions of T cells with two antigen-presenting cell (APC) populations, B cells and dendritic cells (DCs), in the presence of B7- and CD40-dependent co-stimulatory pathways. Contrary to the prevailing paradigm, we found unique cellular requirements for B7 and CD40 expression in primary GC responses to vaccine immunization with protein antigen and adjuvant: B7 was required on DCs but was not required on B cells, whereas CD40 was required on B cells but not on DCs in the generation of antigen-specific follicular helper T cells, antigen-specific GC B cells, and high-affinity class-switched antibody production. There was, in fact, no requirement for coexpression of B7 and CD40 on the same cell in these responses. Our findings support a substantially revised model for co-stimulatory function in the primary GC response, with crucial and distinct contributions of B7- and CD40-dependent pathways expressed by different APC populations and with important implications for understanding how to optimize vaccine responses or limit autoimmunity.
[Mh] Termos MeSH primário: Células Apresentadoras de Antígenos/fisiologia
Antígenos B7/fisiologia
Antígenos CD40/fisiologia
Centro Germinativo/fisiologia
[Mh] Termos MeSH secundário: Animais
Formação de Anticorpos/fisiologia
Linfócitos B/fisiologia
Células Dendríticas/fisiologia
Imunoglobulina G/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Especificidade do Receptor de Antígeno de Linfócitos T/fisiologia
Linfócitos T/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7 Antigens); 0 (CD40 Antigens); 0 (Immunoglobulin G)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1084/jem.20161955


  9 / 5460 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28767735
[Au] Autor:Sia JK; Bizzell E; Madan-Lala R; Rengarajan J
[Ad] Endereço:Emory Vaccine Center, Emory University, Atlanta, GA, United States of America.
[Ti] Título:Engaging the CD40-CD40L pathway augments T-helper cell responses and improves control of Mycobacterium tuberculosis infection.
[So] Source:PLoS Pathog;13(8):e1006530, 2017 Aug.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterium tuberculosis (Mtb) impairs dendritic cell (DC) functions and induces suboptimal antigen-specific CD4 T cell immune responses that are poorly protective. Mucosal T-helper cells producing IFN-γ (Th1) and IL-17 (Th17) are important for protecting against tuberculosis (TB), but the mechanisms by which DCs generate antigen-specific T-helper responses during Mtb infection are not well defined. We previously reported that Mtb impairs CD40 expression on DCs and restricts Th1 and Th17 responses. We now demonstrate that CD40-dependent costimulation is required to generate IL-17 responses to Mtb. CD40-deficient DCs were unable to induce antigen-specific IL-17 responses after Mtb infection despite the production of Th17-polarizing innate cytokines. Disrupting the interaction between CD40 on DCs and its ligand CD40L on antigen-specific CD4 T cells, genetically or via antibody blockade, significantly reduced antigen-specific IL-17 responses. Importantly, engaging CD40 on DCs with a multimeric CD40 agonist (CD40LT) enhanced antigen-specific IL-17 generation in ex vivo DC-T cell co-culture assays. Further, intratracheal instillation of Mtb-infected DCs treated with CD40LT significantly augmented antigen-specific Th17 responses in vivo in the lungs and lung-draining lymph nodes of mice. Finally, we show that boosting CD40-CD40L interactions promoted balanced Th1/Th17 responses in a setting of mucosal DC transfer, and conferred enhanced control of lung bacterial burdens following aerosol challenge with Mtb. Our results demonstrate that CD40 costimulation by DCs plays an important role in generating antigen-specific Th17 cells and targeting the CD40-CD40L pathway represents a novel strategy to improve adaptive immunity to TB.
[Mh] Termos MeSH primário: Antígenos CD40/imunologia
Ligante de CD40/imunologia
Células Dendríticas/imunologia
Ativação Linfocitária/imunologia
Linfócitos T Auxiliares-Indutores/imunologia
Tuberculose/imunologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cocultura
Células Dendríticas/microbiologia
Modelos Animais de Doenças
Citometria de Fluxo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Mycobacterium tuberculosis/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD40 Antigens); 147205-72-9 (CD40 Ligand)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006530


  10 / 5460 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28715682
[Au] Autor:Kraaij MD; van Dijk A; Haagsman HP
[Ad] Endereço:Division of Molecular Host Defence, Dept. of Infectious Diseases & Immunology, Utrecht University, Utrecht, The Netherlands.
[Ti] Título:CATH-2 and LL-37 increase mannose receptor expression, antigen presentation and the endocytic capacity of chicken mononuclear phagocytes.
[So] Source:Mol Immunol;90:118-125, 2017 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cathelicidins display in vitro and in vivo immunomodulatory activities and are part of the innate immune system. Previously, we found that in ovo treatment with chicken cathelicidin CATH-2 partially protects young broilers against respiratory E. coli infection. To determine the cellular aspects of this protection, we investigated immunomodulatory effects of CATH-2 and the human cathelicidin LL-37 on primary chicken peripheral blood mononuclear cells (PBMCs). Treatment of chicken PBMCs with L-CATH-2, D-CATH-2 or LL-37 increased the percentage of mononuclear phagocytes, but decreased that of B cells. L-CATH-2, D-CATH-2 and LL-37 treatment of chicken PBMCs also enhanced the expression levels of mannose receptor MRC1 and antigen presentation markers MHCII, CD40 and CD86 on mononuclear phagocytes, indicating increased antigen presentation capacity. Concomitantly, L-CATH-2, D-CATH-2 and LL-37 neutralized LPS-induced cytokine production, while increasing the endocytic capacity. We conclude that L-CATH-2, D-CATH-2 and LL-37 can modulate the immune response of primary chicken immune cells by increasing mannose receptor expression, antigen presentation, endocytosis and neutralizing LPS-induced cytokine production and as a result augment activation of the adaptive immune system.
[Mh] Termos MeSH primário: Apresentação do Antígeno/imunologia
Peptídeos Catiônicos Antimicrobianos/imunologia
Endocitose/imunologia
Lectinas Tipo C/biossíntese
Lectinas de Ligação a Manose/biossíntese
Receptores de Superfície Celular/biossíntese
[Mh] Termos MeSH secundário: Animais
Antígeno B7-2/metabolismo
Antígenos CD40/metabolismo
Proliferação Celular
Células Cultivadas
Galinhas
Citocinas/biossíntese
Seres Humanos
Lectinas Tipo C/imunologia
Lectinas de Ligação a Manose/imunologia
Sistema Fagocitário Mononuclear/imunologia
Receptores de Superfície Celular/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (B7-2 Antigen); 0 (CD40 Antigens); 0 (CMAP27 protein, chicken); 0 (Cytokines); 0 (Lectins, C-Type); 0 (Mannose-Binding Lectins); 0 (Receptors, Cell Surface); 0 (mannose receptor); 143108-26-3 (CAP18 lipopolysaccharide-binding protein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE



página 1 de 546 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde