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  1 / 133 MEDLINE  
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[PMID]:28817184
[Au] Autor:Albayati Z; Alyami A; Alomar S; Middleton D; Bonnett L; Aleem S; Flanagan BF; Christmas SE
[Ad] Endereço:Department of Clinical Infection, Microbiology & Immunology, Institute of Infection & Global Health, University of Liverpool, Liverpool, UK.
[Ti] Título:The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
[So] Source:Scand J Immunol;86(5):396-407, 2017 Nov.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:HLA-G is a non-classical class I HLA antigen, normally expressed in high levels only on extravillous cytotrophoblast. It has immunosuppressive properties in pregnancy and has also been found to be upregulated on leucocytes in viral infection. In this study, proportions of all leucocyte subsets expressing HLA-G were found to be low in healthy subjects positive or negative for cytomegalovirus (CMV). Significantly greater proportions of CD4+ CD69+ and CD56+ T cells expressed HLA-G compared to other T cells. However, following stimulation with CMV antigens or intact CMV, proportions of CD4+, CD8+, CD69+ and CD56+ T cells, and also B cells expressing HLA-G, were significantly increased in CMV+ subjects. Despite some subjects having alleles of HLA-G associated with high levels of expression, no relationship was found between HLA-G genotype and expression levels. Purified B cells from CMV+ subjects stimulated in mixed culture with CMV antigens showed significantly increased HLA-G mRNA expression by real-time polymerase chain reaction. Serum levels of soluble HLA-G were similar in CMV- and CMV+ subjects but levels in culture supernatants were significantly higher in cells from CMV+ than from CMV- subjects stimulated with CMV antigens. The HLA-G ligand KIR2DL4 was mainly expressed on NK cells and CD56+ T cells with no differences between CMV+ and CMV- subjects. Following stimulation with IL-2, an increase in the proportion of CD56+ T cells positive for KIR2DL4 was found, together with a significant decrease in CD56dimCD16+ NK cells. The results show that CMV influences HLA-G expression in healthy subjects and may contribute to viral immune evasion.
[Mh] Termos MeSH primário: Citomegalovirus/imunologia
Antígenos HLA-G/metabolismo
Leucócitos/imunologia
Leucócitos/virologia
Receptores KIR2DL4/metabolismo
[Mh] Termos MeSH secundário: Adulto
Anticorpos Antivirais/sangue
Antígenos Virais/administração & dosagem
Proliferação Celular
Citomegalovirus/patogenicidade
Infecções por Citomegalovirus/genética
Infecções por Citomegalovirus/imunologia
Feminino
Antígenos HLA-G/genética
Seres Humanos
Evasão da Resposta Imune
Técnicas In Vitro
Células Matadoras Naturais/imunologia
Células Matadoras Naturais/virologia
Leucócitos/classificação
Ligantes
Masculino
Meia-Idade
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Receptores KIR2DL4/genética
Subpopulações de Linfócitos T/imunologia
Subpopulações de Linfócitos T/virologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (HLA-G Antigens); 0 (KIR2DL4 protein, human); 0 (Ligands); 0 (RNA, Messenger); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12594


  2 / 133 MEDLINE  
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[PMID]:28397235
[Au] Autor:Zhang G; Deng Z
[Ad] Endereço:Southern Medical University, Guangzhou, Guangdong 510515, China; Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. zhihui_deng@aliyun.com.
[Ti] Título:[Allelic diversity of KIR2DL4 gene and identification of five novel alleles among southern Han Chinese population].
[So] Source:Zhonghua Yi Xue Yi Chuan Xue Za Zhi;34(2):270-274, 2017 Apr 10.
[Is] ISSN:1003-9406
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To study the polymorphisms of the KIR2DL4 gene in a southern Han Chinese population. METHODS: Genomic DNA isolated from 306 unrelated individuals was amplified by using KIR2DL4-specific PCR primers. The PCR products were genotyped for the entire coding sequence by sequencing-based typing (SBT). Assignment of allelic profile was accomplished with an Assign 3.5 software. For samples with inconclusive SBT results, the RT-PCR products covering the entire coding sequence of the KIR2DL4 gene were subjected to cloning and haplotype sequencing. RESULTS: Among the 306 individuals, 11 alleles were detected, of which 5 novel alleles were officially named by the KIR subcommittee of the World Health Organization Nomenclature Committee for factors of HLA system. The observed frequencies for the 11 alleles were KIR2DL4 *00102 (75.5%), *00103 (8.2%), *00501 (34.0%), *00503 (0.7%), *00504 (0.7%), *00602 (14.4%), *00801 (11.4%), *011 (22.2%), *032 (0.3%), *033 (0.3%) and *034 (0.3%). The ratio of 10A type alleles including 2DL4*00102, *00103, *00501, *00503, *00504, *00602, *032, *033, *034 and 9A type alleles including 2DL4*00801, *011 were 97.0% and 33.0%, respectively, with a ratio of 2.9:1. CONCLUSION: The allelic diversity of the KIR2DL4 gene in southern Han Chinese has been elucidated, which may provide valuable data for research on transplantation, reproductive immunity, KIR-associated disease and evolution.
[Mh] Termos MeSH primário: Variação Genética
Receptores KIR2DL4/genética
[Mh] Termos MeSH secundário: Alelos
Grupo com Ancestrais do Continente Asiático/etnologia
Grupo com Ancestrais do Continente Asiático/genética
Sequência de Bases
China/etnologia
Frequência do Gene
Genótipo
Haplótipos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (KIR2DL4 protein, human); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1003-9406.2017.02.027


  3 / 133 MEDLINE  
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[PMID]:27320605
[Au] Autor:Eguchi H; Maeda A; Lo PC; Matsuura R; Esquivel EL; Asada M; Sakai R; Nakahata K; Yamamichi T; Umeda S; Deguchi K; Ueno T; Okuyama H; Miyagawa S
[Ad] Endereço:Department of Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. Electronic address: hiroshieguchi@hotmail.com.
[Ti] Título:HLA-G1, but Not HLA-G3, Suppresses Human Monocyte/Macrophage-mediated Swine Endothelial Cell Lysis.
[So] Source:Transplant Proc;48(4):1285-7, 2016 May.
[Is] ISSN:1873-2623
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The inhibitory function of HLA-G1, a class Ib molecule, on monocyte/macrophage-mediated cytotoxicity was examined. The expression of inhibitory receptors that interact with HLA-G, immunoglobulin-like transcript 2 (ILT2), ILT4, and KIR2DL4 (CD158d) on in vitro-generated macrophages obtained from peripheral blood mononuclear cells and the phorbol 12-myristate 13-acetate (PMA)-activated THP-1 cells were examined by flow cytometry. cDNAs of HLA-G1, HLA-G3, HLA-E, and human ß2-microglobulin were prepared, transfected into pig endothelial cells (PECs), and macrophage- and the THP-1 cell-mediated PEC cytolysis was then assessed. In vitro-generated macrophages expressed not only ILT2 and ILT4 but CD158d as well. The transgenic HLA-G1 on PEC indicated a significant suppression in macrophage-mediated cytotoxicity, which was equivalent to that of transgenic HLA-E. HLA-G1 was clearly expressed on the cell surface of PEC, whereas the levels of HLA-G3 were much lower and remained in the intracellular space. On the other hand, the PMA-activated THP-1 cell was less expressed these inhibitory molecules than in vitro-generated macrophages. Therefore, the HLA-G1 on PECs showed a significant but relatively smaller suppression to THP-1 cell-mediated cytotoxicity compared to in vitro-generated macrophages. These results indicate that by generating HLA-G1, but not HLA-G3, transgenic pigs can protect porcine grafts from monocyte/macrophage-mediated cytotoxicity.
[Mh] Termos MeSH primário: Antígenos HLA-G/fisiologia
Leucócitos Mononucleares/imunologia
Macrófagos/imunologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Antígenos CD/metabolismo
Citocinas/metabolismo
Citotoxicidade Imunológica/fisiologia
Células Endoteliais/imunologia
Endotélio/imunologia
Citometria de Fluxo
Antígenos HLA-G/metabolismo
Seres Humanos
Células Matadoras Naturais/imunologia
Receptor B1 de Leucócitos Semelhante a Imunoglobulina
Glicoproteínas de Membrana/metabolismo
Receptores Imunológicos/metabolismo
Receptores KIR2DL4/metabolismo
Suínos
Transfecção/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Cytokines); 0 (HLA-G Antigens); 0 (KIR2DL4 protein, human); 0 (LILRB1 protein, human); 0 (LILRB2 protein, human); 0 (Leukocyte Immunoglobulin-like Receptor B1); 0 (Membrane Glycoproteins); 0 (Receptors, Immunologic); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160621
[St] Status:MEDLINE


  4 / 133 MEDLINE  
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[PMID]:26973020
[Au] Autor:Nowak I; Malinowski A; Barcz E; Wilczynski JR; Wagner M; Majorczyk E; Motak-Pochrzest H; Banasik M; Kusnierczyk P
[Ad] Endereço:Laboratory of Immunogenetics and Tissue Immunology, Department of Clinical Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, Poland. izan@iitd.pan.wroc.pl.
[Ti] Título:Possible Role of HLA-G, LILRB1 and KIR2DL4 Gene Polymorphisms in Spontaneous Miscarriage.
[So] Source:Arch Immunol Ther Exp (Warsz);64(6):505-514, 2016 Dec.
[Is] ISSN:1661-4917
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The KIR2DL4 receptor and its ligand HLA-G are considered important for fetal-maternal immune tolerance and successful pregnancy. The absence of a particular variant of KIR2DL4 might be a bad prognostic factor for pregnancy outcome. However, it could be compensated by the presence of the respective LILRB1 allele. Therefore, we investigated the KIR2DL4, LILRB1 and HLA-G polymorphisms in 277 couples with spontaneous abortion and 219 control couples by HRM, PCR-SSP and RFLP methods. We found a protective effect of women's heterozygosity in -716 HLA-G (p = 0.0206) and LILRB1 (p = 0.0131) against spontaneous abortion. Surprisingly, we observed more 9A/10A genotypes of KIR2DL4 gene carriers in the group of male partners from the miscarriage group in comparison to the men from the control group (p = 0.0288). Furthermore, there was no association of women's KIR2DL4 polymorphism with susceptibility to spontaneous abortion. Multivariate analysis indicated that women's -716 HLA-G and LILRB1 and men's KIR2DL4 9A/10A are important in terms of the protection or susceptibility to miscarriage, respectively (p = 0.00968). In conclusion, a woman's heterozygosity in HLA-G and LILRB1 might be an advantage for a success of reproduction, but the partner's heterozygosity in 9A/10A KIR2DL4 alleles might not.
[Mh] Termos MeSH primário: Aborto Espontâneo/genética
Aborto Espontâneo/imunologia
Antígenos CD/fisiologia
Antígenos HLA-G/fisiologia
Polimorfismo de Nucleotídeo Único
Receptores Imunológicos/fisiologia
Receptores KIR2DL4/fisiologia
[Mh] Termos MeSH secundário: Aborto Habitual/genética
Aborto Habitual/imunologia
Adulto
Idoso
Alelos
Antígenos CD/genética
Estudos de Casos e Controles
Feminino
Genótipo
Antígenos HLA-G/genética
Haplótipos
Heterozigoto
Seres Humanos
Tolerância Imunológica
Receptor B1 de Leucócitos Semelhante a Imunoglobulina
Desequilíbrio de Ligação
Masculino
Meia-Idade
Modelos Estatísticos
Análise Multivariada
Polimorfismo Genético
Gravidez
Resultado da Gravidez
Receptores Imunológicos/genética
Receptores KIR2DL4/genética
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (HLA-G Antigens); 0 (KIR2DL4 protein, human); 0 (LILRB1 protein, human); 0 (Leukocyte Immunoglobulin-like Receptor B1); 0 (Receptors, Immunologic); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160315
[St] Status:MEDLINE


  5 / 133 MEDLINE  
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[PMID]:26917249
[Au] Autor:Alicata C; Bottino C; Guethlein LA; Parham P; Norman PJ
[Ad] Endereço:Dipartimento di Medicina Sperimentale, University of Genoa, Genoa, Italy.
[Ti] Título:Description of the novel KIR2DL4*035 allele identified using high-throughput sequencing.
[So] Source:HLA;87(3):191-3, 2016 Mar.
[Is] ISSN:2059-2310
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A newly identified allele of the KIR2DL4 natural killer cell receptor for human leukocyte antigen (HLA) class I.
[Mh] Termos MeSH primário: Alelos
Éxons
Mutação Puntual
Receptores KIR2DL4/genética
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Sequência de Bases
Códon
Haplótipos
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Células Matadoras Naturais/citologia
Células Matadoras Naturais/imunologia
Tipagem Molecular
Receptores KIR2DL4/imunologia
Alinhamento de Sequência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon); 0 (KIR2DL4 protein, human); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE
[do] DOI:10.1111/tan.12761


  6 / 133 MEDLINE  
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[PMID]:26823774
[Au] Autor:Wang D; Tian Y; Zhao Y; Liu L; Liu X; Wu F
[Ad] Endereço:Department of Obstetrics and Gynecology, The Second Hospital of Jilin University Changchun 130041, China.
[Ti] Título:KIR2DL4 expression rather than its single nucleotide polymorphisms correlates with pre-eclampsia.
[So] Source:Int J Clin Exp Pathol;8(11):14535-41, 2015.
[Is] ISSN:1936-2625
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the single nucleotide polymorphisms and expression of KIR2DL4 (killer cell immunoglobulin-like receptors) gene in pre-eclampsia patients. METHODS: KIR2DL4 gene polymorphisms were detected in 100 patients with pre-eclampsia and 100 healthy pregnant women, respectively, by using PCR-SS. Then, the expression of KIR2DL4 was measured in 5 cases of placentas tissues with pre-eclampsia and normal pregnancies by using qRT-PCR. RESULTS: Compared with healthy controls, 16 loci of single nucleotide polymorphisms (SNP) were identified in pre-eclampsia patients, including 7 new polymorphisms loci. But, no significant difference was found in genotype distributions and allele frequencies in pre-eclampsia and controls (P>0.05). However, qRT-PCR results showed that KIR2DL4 mRNA in placenta tissues with pre-eclampsia was significantly lower than those with normal pregnancy, and the difference was statistically significant. CONCLUSION: Decreased level of KIR2DL4 rather than its SNP is correlated with the susceptibility of pre-eclampsia.
[Mh] Termos MeSH primário: Predisposição Genética para Doença/genética
Pré-Eclâmpsia/genética
Receptores KIR2DL4/genética
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Feminino
Genótipo
Seres Humanos
Polimorfismo de Nucleotídeo Único
Gravidez
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (KIR2DL4 protein, human); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160130
[St] Status:MEDLINE


  7 / 133 MEDLINE  
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[PMID]:26293482
[Au] Autor:Djurisic S; Skibsted L; Hviid TV
[Ad] Endereço:Department of Clinical Biochemistry, Centre for Immune Regulation and Reproductive Immunology (CIRRI), Copenhagen University Hospital (Roskilde), University of Copenhagen, Roskilde, Denmark.
[Ti] Título:A Phenotypic Analysis of Regulatory T Cells and Uterine NK Cells from First Trimester Pregnancies and Associations with HLA-G.
[So] Source:Am J Reprod Immunol;74(5):427-44, 2015 Nov.
[Is] ISSN:1600-0897
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:PROBLEM: The prevalence of regulatory T cells and NK cells expressing activation and HLA-G receptors, and the influence of in vivo sHLA-G and mHLAG on HLA-G receptors expressed by NK cells in the uterine compartment is unclear. METHOD OF STUDY: KIR2DL4 and/or ILT2 expression on regulatory T cells and NK cells from the placental bed and peripheral blood in first trimester was assessed using flow cytometry. Expression of mHLA-G on trophoblast cells and sHLA-G in 'uterine' and peripheral blood was determined with ELISA and flow cytometry, and specific associations with expression levels of cognate receptors or activation markers on immune cells were determined. RESULTS: In the placental bed, CD45RA surface expression on Tregs was similar to peripheral Tregs in pregnant women, but T cells with lower CD4 and CD8 expression were accumulated. HLA-G receptor expression was increased on NK cells from 'uterine blood'. Soluble HLA-G was significantly increased in 'uterine blood' compared with peripheral blood, but no correlation was found between sHLA-G and mHLA-G in the uterine compartment. A correlation was found between sHLA-G and the fraction of KIR2DL4-positive NK cells in the uterine compartment, and a tendency was observed between mHLA-G and the fraction of ILT2-positive NK cells in the uterine compartment. CONCLUSION: The NK subset in the placental bed displays a unique phenotype that may be influenced by mHLA-G on trophoblast cells and locally accumulated sHLA-G in the uterus.
[Mh] Termos MeSH primário: Antígenos CD/metabolismo
Antígenos HLA-G/metabolismo
Células Matadoras Naturais/metabolismo
Primeiro Trimestre da Gravidez/metabolismo
Receptores Imunológicos/metabolismo
Receptores KIR2DL4/metabolismo
Linfócitos T Reguladores/metabolismo
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Células Matadoras Naturais/citologia
Receptor B1 de Leucócitos Semelhante a Imunoglobulina
Gravidez
Linfócitos T Reguladores/citologia
Trofoblastos/citologia
Trofoblastos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (HLA-G Antigens); 0 (KIR2DL4 protein, human); 0 (LILRB1 protein, human); 0 (Leukocyte Immunoglobulin-like Receptor B1); 0 (Receptors, Immunologic); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150822
[St] Status:MEDLINE
[do] DOI:10.1111/aji.12421


  8 / 133 MEDLINE  
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[PMID]:26187179
[Au] Autor:Biedron M; Rybka J; Wróbel T; Prajs I; Poreba R; Kuliczkowski K
[Ad] Endereço:Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Wroclaw Medical University, ul. Pasteura 4, 50-367, Wroclaw, Poland.
[Ti] Título:The role of soluble HLA-G and HLA-G receptors in patients with hematological malignancies after allogeneic stem cell transplantation.
[So] Source:Med Oncol;32(8):219, 2015 Aug.
[Is] ISSN:1559-131X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HLA-G is a non-classical MHC class I molecule whose suppressive activity on immune effector cells is exerted due to interactions with receptors ILT2, ILT4 and KIR2DL4. These receptors are expressed mainly on NK cells and monocytes, and their intensity of expression changes depending on HLA-G level. HLA-G plays an important role in the development of tolerance following organ transplantations and bone marrow stem cell transplantations. HLA-G also participates in the modulation of the immune response during cancerogenesis. The aim of this study was to assess HLA-G level in blood serum, the percentage of NK cells and monocytes with expression of receptors for HLA-G (ILT2, ILT4, KIR2DL4 and NKG2D) in patients who received allogeneic stem cell transplantations, and their influence on the occurrence of graft-versus-host reaction. The study included 32 patients with bone marrow diseases (acute leukemias, myelodysplastic syndrome, chronic myeloid leukemia, paroxysmal nocturnal hemoglobinuria) who received allogeneic stem cell transplantations. We assessed the expression of receptors ILT2, ILT4, KIR2DL4 and NKG2D on monocytes and NK cells, as well as the level of HLA-G in blood serum in patients before conditioning, in the transplant hematopoietic reconstitution period following allogeneic bone marrow stem cell transplantation. The percentage of NK cells with expression of KIR2DL4, ILT2 and ILT4 receptors was higher in patients with 0-I grade GVHD than in patients with II-IV grade GVHD. The percentage of monocytes with expression of ILT4 and ILT2 receptors was higher in patients with 0-I grade GVHD than in patients with II-IV grade GVHD. The level of HLA-G in patients' blood serum was higher after the stem cell transplantation compared with the period before transplantation. HLA-G level and HLA-G receptors are related to intensity of GVHD and may play the role of a prognostic factor for the development of GVHD and the clinical course of this reaction.
[Mh] Termos MeSH primário: Antígenos HLA-G/sangue
Neoplasias Hematológicas/cirurgia
Transplante de Células-Tronco Hematopoéticas
Receptores Imunológicos/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antígenos CD/sangue
Transplante de Medula Óssea
Feminino
Doença Enxerto-Hospedeiro/imunologia
Neoplasias Hematológicas/imunologia
Seres Humanos
Células Matadoras Naturais/imunologia
Receptor B1 de Leucócitos Semelhante a Imunoglobulina
Masculino
Glicoproteínas de Membrana/sangue
Meia-Idade
Monócitos/imunologia
Subfamília K de Receptores Semelhantes a Lectina de Células NK/sangue
Receptores KIR2DL4/sangue
Transplante Homólogo
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (HLA-G Antigens); 0 (KIR2DL4 protein, human); 0 (KLRK1 protein, human); 0 (LILRB1 protein, human); 0 (LILRB2 protein, human); 0 (Leukocyte Immunoglobulin-like Receptor B1); 0 (Membrane Glycoproteins); 0 (NK Cell Lectin-Like Receptor Subfamily K); 0 (Receptors, Immunologic); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150719
[St] Status:MEDLINE
[do] DOI:10.1007/s12032-015-0664-1


  9 / 133 MEDLINE  
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[PMID]:25855135
[Au] Autor:Wisniewski A; Kowal A; Wyrodek E; Nowak I; Majorczyk E; Wagner M; Pawlak-Adamska E; Jankowska R; Slesak B; Frydecka I; Kusnierczyk P
[Ad] Endereço:Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
[Ti] Título:Genetic polymorphisms and expression of HLA-G and its receptors, KIR2DL4 and LILRB1, in non-small cell lung cancer.
[So] Source:Tissue Antigens;85(6):466-75, 2015 Jun.
[Is] ISSN:1399-0039
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human leukocyte antigen-G (HLA-G) is a nonclassical HLA class I molecule absent from most normal tissues but detected in many malignant tumors. It is recognized by cells of the immune system using LILRB1, KIR2DL4 and LILRB2 receptors. We attempted to find out whether some polymorphisms of HLA-G, LILRB1 and KIR2DL4 genes are associated with susceptibility to nonsmall cell lung cancer (NSCLC). Four polymorphisms in HLA-G, i.e. -964A>G (rs1632947), -725C>G>T (rs1233334), -716T>G (rs2249863) in the promoter, and a 14 base pair insertion/deletion (14 bp indel) in the 3'-untranslated region (3'UTR), and five in LILRB1 - 5651G>A (rs41308748) in intron 14, 5717C>T L622L (rs1061684), 5724G>A E625K (rs16985478), 5774 C>A P641P (rs41548213) in exon 15, and 5806C>T (rs8101240) in 3'UTR - as well as 9620 9A/10A (rs11410751) polymorphism in exon 7 of KIR2DL4 were typed using different laboratory techniques. Only one single nucleotide polymorphism (SNP) in HLA-G (-964A>G) and one in LILRB1 (5724G>A) were found to influence the risk of NSCLC. In addition, 5724G>A was associated with protection from tumor cell infiltration of regional lymph nodes. Most importantly, we detected HLA-G and LILRB1 expression in tumor specimens, but no correlation with genetic polymorphisms was observed. HLA-G and LILRB1 protein expression levels in tumor tissue were significantly correlated with tumor stage.
[Mh] Termos MeSH primário: Antígenos CD/genética
Antígenos de Neoplasias/genética
Carcinoma Pulmonar de Células não Pequenas/genética
Antígenos HLA-G/genética
Mutação INDEL
Neoplasias Pulmonares/genética
Proteínas de Neoplasias/genética
Polimorfismo de Nucleotídeo Único
Receptores Imunológicos/genética
Receptores KIR2DL4/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Substituição de Aminoácidos
Antígenos CD/biossíntese
Antígenos CD/imunologia
Antígenos de Neoplasias/biossíntese
Antígenos de Neoplasias/imunologia
Biomarcadores Tumorais
Carcinoma Pulmonar de Células não Pequenas/epidemiologia
Carcinoma Pulmonar de Células não Pequenas/patologia
Feminino
Perfilação da Expressão Gênica
Frequência do Gene
Antígenos HLA-G/biossíntese
Antígenos HLA-G/imunologia
Seres Humanos
Receptor B1 de Leucócitos Semelhante a Imunoglobulina
Neoplasias Pulmonares/epidemiologia
Neoplasias Pulmonares/patologia
Metástase Linfática
Masculino
Meia-Idade
Invasividade Neoplásica
Proteínas de Neoplasias/biossíntese
Proteínas de Neoplasias/imunologia
Estadiamento de Neoplasias
Regiões Promotoras Genéticas/genética
Receptores Imunológicos/biossíntese
Receptores Imunológicos/imunologia
Receptores KIR2DL4/biossíntese
Receptores KIR2DL4/imunologia
Risco
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Neoplasm); 0 (Biomarkers, Tumor); 0 (HLA-G Antigens); 0 (KIR2DL4 protein, human); 0 (LILRB1 protein, human); 0 (Leukocyte Immunoglobulin-like Receptor B1); 0 (Neoplasm Proteins); 0 (Receptors, Immunologic); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150410
[St] Status:MEDLINE
[do] DOI:10.1111/tan.12561


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[PMID]:25818657
[Au] Autor:Nowak I; Barcz E; Majorczyk E; Malinowski A; Wilczynski JR; Banasik M; Motak-Pochrzest H; Kusnierczyk P
[Ad] Endereço:Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
[Ti] Título:Genetic polymorphism of KIR2DL4 in the Polish population.
[So] Source:Tissue Antigens;85(6):450-7, 2015 Jun.
[Is] ISSN:1399-0039
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The KIR2DL4 gene is characterized by alleles with either 9 or 10 consecutive adenines in exon 7, which encodes the transmembrane domain. The 9A variant produces either a protein with a truncated cytoplasmic tail or one lacking the transmembrane region. This causes a lack of KIR2DL4 expression. In contrast, 10A alleles encode receptors that may be expressed at the cell surface. We tested 438 healthy individuals for polymorphism of the KIR2DL4 gene. KIR2DL4 9A/10A alleles were distinguished by the high resolution melting (HRM) method, and restriction fragment length polymorphism (RFLP) was used for genotyping of three other single nucleotide polymorphisms (SNPs) spanning the near vicinity of the poly-adenine fragment. We found a weak difference between males and females in 9769 C/A genotypes and alleles. In addition, we observed complete linkage disequilibrium (LD) between 9A insertion/deletion in the 9620 position and the 9571T/C position of the gene (r(2) = 1) both in females and males and almost complete LD with the 9797G/A position (r(2) = 0.963 for females and r(2) = 0.892 for males). Most importantly, we detected, in a group of fertile women, a high frequency (30.2%) of homozygosity for the defective 9A variant, which suggests that KIR2DL4 as a functional cell surface receptor is not absolutely necessary for reproduction. On the other hand, lower representation of 10A/10A homozygotes and high frequency of 10A/9A heterozygotes indicates a need for both cell membrane-anchored and soluble KIR2DL4 molecules. Finally, cost-reducing RFLP instead of HRM is proposed for typing 9A and 10A variants.
[Mh] Termos MeSH primário: Receptores KIR2DL4/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Alelos
Antígenos de Superfície/genética
Sequência de Bases
Éxons/genética
Feminino
Fertilidade/genética
Frequência do Gene
Genótipo
Técnicas de Genotipagem/economia
Seres Humanos
Desequilíbrio de Ligação
Masculino
Meia-Idade
Dados de Sequência Molecular
Desnaturação de Ácido Nucleico
Polônia
Polimorfismo de Fragmento de Restrição
Polimorfismo de Nucleotídeo Único
Estrutura Terciária de Proteína
Receptores KIR2DL4/fisiologia
Caracteres Sexuais
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Surface); 0 (KIR2DL4 protein, human); 0 (Receptors, KIR2DL4)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150505
[Lr] Data última revisão:
150505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE
[do] DOI:10.1111/tan.12544



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