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[PMID]: 28644614 [Au] Autor: Zyuzin MV; Yan Y; Hartmann R; Gause KT; Nazarenus M; Cui J; Caruso F; Parak WJ [Ad] Endereço: Fachbereich Physik, Philipps-Universität Marburg , 35037 Marburg, Germany. [Ti] Título: Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells. [So] Source: Bioconjug Chem;28(8):2062-2068, 2017 Aug 16. [Is] ISSN: 1520-4812 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle-cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of exposure to particles. These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein-based particulate materials exposed to blood plasma. The ability to control the cellular recognition of these organic particles is expected to aid the advancement of HSA-based materials for intravenous drug delivery. [Mh] Termos MeSH primário: Células Endoteliais da Veia Umbilical Humana/metabolismo Nanoporos Coroa de Proteína/química Coroa de Proteína/metabolismo Albumina Sérica/química Albumina Sérica/metabolismo [Mh] Termos MeSH secundário: Seres Humanos Lisossomos/metabolismo Transporte Proteico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Protein Corona); 0 (Serum Albumin) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170912 [Lr] Data última revisão: 170912 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170624 [St] Status: MEDLINE [do] DOI: 10.1021/acs.bioconjchem.7b00231

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[PMID]: 28420299 [Au] Autor: Chandran P; Riviere JE; Monteiro-Riviere NA [Ad] Endereço: a Department of Anatomy and Physiology, Nanotechnology Innovation Center of Kansas State , Kansas State University , Manhattan , KS , USA. [Ti] Título: Surface chemistry of gold nanoparticles determines the biocorona composition impacting cellular uptake, toxicity and gene expression profiles in human endothelial cells. [So] Source: Nanotoxicology;11(4):507-519, 2017 May. [Is] ISSN: 1743-5404 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: This study investigated the role of nanoparticle size and surface chemistry on biocorona composition and its effect on uptake, toxicity and cellular responses in human umbilical vein endothelial cells (HUVEC), employing 40 and 80 nm gold nanoparticles (AuNP) with branched polyethyleneimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coatings. Proteomic analysis identified 59 hard corona proteins among the various AuNP, revealing largely surface chemistry-dependent signature adsorbomes exhibiting human serum albumin (HSA) abundance. Size distribution analysis revealed the relative instability and aggregation inducing potential of bare and corona-bound BPEI-AuNP, over LA- and PEG-AuNP. Circular dichroism analysis showed surface chemistry-dependent conformational changes of proteins binding to AuNP. Time-dependent uptake of bare, plasma corona (PC) and HSA corona-bound AuNP (HSA-AuNP) showed significant reduction in uptake with PC formation. Cell viability studies demonstrated dose-dependent toxicity of BPEI-AuNP. Transcriptional profiling studies revealed 126 genes, from 13 biological pathways, to be differentially regulated by 40 nm bare and PC-bound BPEI-AuNP (PC-BPEI-AuNP). Furthermore, PC formation relieved the toxicity of cationic BPEI-AuNP by modulating expression of genes involved in DNA damage and repair, heat shock response, mitochondrial energy metabolism, oxidative stress and antioxidant response, and ER stress and unfolded protein response cascades, which were aberrantly expressed in bare BPEI-AuNP-treated cells. NP surface chemistry is shown to play the dominant role over size in determining the biocorona composition, which in turn modulates cell uptake, and biological responses, consequently defining the potential safety and efficacy of nanoformulations. [Mh] Termos MeSH primário: Células Endoteliais/efeitos dos fármacos Ouro Nanopartículas Metálicas Coroa de Proteína/metabolismo Transcriptoma/efeitos dos fármacos [Mh] Termos MeSH secundário: Albuminas/metabolismo Sobrevivência Celular/efeitos dos fármacos Dicroísmo Circular Células Endoteliais/metabolismo Ouro/química Ouro/toxicidade Células Endoteliais da Veia Umbilical Humana Seres Humanos Nanopartículas Metálicas/química Nanopartículas Metálicas/toxicidade Tamanho da Partícula Polietilenoglicóis/química Polietilenoimina/química Proteômica Propriedades de Superfície [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Albumins); 0 (Protein Corona); 30IQX730WE (Polyethylene Glycols); 7440-57-5 (Gold); 9002-98-6 (Polyethyleneimine) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170929 [Lr] Data última revisão: 170929 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170420 [St] Status: MEDLINE [do] DOI: 10.1080/17435390.2017.1314036

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[PMID]: 28414772 [Au] Autor: Lundqvist M; Augustsson C; Lilja M; Lundkvist K; Dahlbäck B; Linse S; Cedervall T [Ad] Endereço: Center for Molecular Protein Science, Biochemistry, Lund University, Lund, Sweden. [Ti] Título: The nanoparticle protein corona formed in human blood or human blood fractions. [So] Source: PLoS One;12(4):e0175871, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The protein corona formed around nanoparticles in protein-rich fluids plays an important role for nanoparticle biocompatibility, as found in several studies during the last decade. Biological fluids have complex compositions and the molecular components interact and function together in intricate networks. Therefore, the process to isolate blood or the preparation of blood derivatives may lead to differences in the composition of the identified protein corona around nanoparticles. Here, we show distinct differences in the protein corona formed in whole blood, whole blood with EDTA, plasma, or serum. Furthermore, the ratio between particle surface area to protein concentration influences the detected corona. We also show that the nanoparticle size per se influences the formed protein corona due to curvature effects. These results emphasize the need of investigating the formation and biological importance of the protein corona in the same environment as the nanoparticles are intended for or released into. [Mh] Termos MeSH primário: Nanopartículas/metabolismo Plasma/metabolismo Coroa de Proteína/metabolismo Soro/metabolismo [Mh] Termos MeSH secundário: Materiais Biocompatíveis/metabolismo Seres Humanos Tamanho da Partícula [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biocompatible Materials); 0 (Protein Corona) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170505 [Lr] Data última revisão: 170505 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170418 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0175871

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[PMID]: 28352171 [Au] Autor: Bewersdorff T; Vonnemann J; Kanik A; Haag R; Haase A [Ad] Endereço: Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany. [Ti] Título: The influence of surface charge on serum protein interaction and cellular uptake: studies with dendritic polyglycerols and dendritic polyglycerol-coated gold nanoparticles. [So] Source: Int J Nanomedicine;12:2001-2019, 2017. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: Nanoparticles (NPs) have gained huge interest in the medical field, in particular for drug delivery purposes. However, binding of proteins often leads to fast NP uptake and rapid clearance, thereby hampering medical applications. Thus, it is essential to determine and control the bio-nano interface. This study investigated the serum protein interactions of dendritic polyglycerols (dPGs), which are promising drug delivery candidates by means of two dimensional gel electrophoresis (2DE) in combination with mass spectrometry. In order to investigate the influence of surface charge, sulfated (sulfated dendritic polyglycerol [dPGS]) and non-sulfated (dPGOH) surfaces were applied, which were synthesized on a gold core allowing for easier separation from unbound biomolecules through centrifugation. Furthermore, two different sizes for dPGS were included. Although size had only a minor influence, considerable differences were detected in protein affinity for dPGS versus dPGOH surfaces, with dPGOH binding much less proteins. Cellular uptake into human CD14 monocytes was analyzed by flow cytometry, and dPGOH was taken up to a much lower extent compared to dPGS. By using a pull-down approach, possible cellular interaction partners of serum pre-incubated dPGS-Au20 NPs from the membrane fraction of THP-1 cells could be identified such as for instance the transferrin receptor or an integrin. Clathrin-mediated endocytosis was further investigated using chlorpromazine as an inhibitor, which resulted in a 50% decrease of the cellular uptake of dPGS. This study could confirm the influence of surface charge on protein interactions and cellular uptake of dPGS. Furthermore, the approach allowed for the identification of possible uptake receptors and insights into the uptake mechanism. [Mh] Termos MeSH primário: Proteínas Sanguíneas/metabolismo Glicerol/farmacocinética Nanopartículas/química Polímeros/farmacocinética [Mh] Termos MeSH secundário: Proteínas Sanguíneas/química Células Cultivadas Clorpromazina/farmacologia Sistemas de Liberação de Medicamentos/métodos Endocitose/efeitos dos fármacos Citometria de Fluxo/métodos Glicerol/química Ouro/química Seres Humanos Receptores de Lipopolissacarídeos/metabolismo Monócitos/efeitos dos fármacos Monócitos/metabolismo Tamanho da Partícula Polímeros/química Coroa de Proteína/química Sulfatos/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Blood Proteins); 0 (Lipopolysaccharide Receptors); 0 (Polymers); 0 (Protein Corona); 0 (Sulfates); 25618-55-7 (polyglycerol); 7440-57-5 (Gold); PDC6A3C0OX (Glycerol); U42B7VYA4P (Chlorpromazine) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170330 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S124295

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[PMID]: 28315770 [Au] Autor: Martínez-Negro M; Caracciolo G; Palchetti S; Pozzi D; Capriotti AL; Cavaliere C; Laganà A; Ortiz Mellet C; Benito JM; García Fernández JM; Aicart E; Junquera E [Ad] Endereço: Grupo de Química Coloidal y Supramolecular, Departamento de Química Física I, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain. [Ti] Título: Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes. Efficient cellular transfection on cancer cells. [So] Source: Biochim Biophys Acta;1861(7):1737-1749, 2017 07. [Is] ISSN: 0006-3002 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The self-assembling processes underlining the capabilities of facially differentiated ("Janus") polycationic amphiphilic cyclodextrins (paCDs) as non-viral gene nanocarriers have been investigated by a pluridisciplinary approach. Three representative Janus paCDs bearing a common tetradecahexanoyl multitail domain at the secondary face and differing in the topology of the cluster of amino groups at the primary side were selected for this study. All of them compact pEGFP-C3 plasmid DNA and promote transfection in HeLa and MCF-7 cells, both in absence and in presence of human serum. The electrochemical and structural characteristics of the paCD-pDNA complexes (CDplexes) have been studied by using zeta potential, DLS, SAXS, and cryo-TEM. paCDs and pDNA, when assembled in CDplexes, render effective charges that are lower than the nominal ones. The CDplexes show a self-assembling pattern corresponding to multilamellar lyotropic liquid crystal phases, characterized by a lamellar stacking of bilayers of the CD-based vectors with anionic pDNA sandwiched among them. When exposed to human serum, either in the absence or in the presence of pDNA, the surface of the cationic CD-based vector becomes coated by a protein corona (PC) whose composition has been analyzed by nanoLC-MS/MS. Some of the CDplexes herein studied showed moderate-to-high transfection levels in HeLa and MCF-7 cancer cells combined with moderate-to-high cell viabilities, as determined by FACS and MTT reduction assays. The ensemble of data provides a detail picture of the paCD-pDNA-PC association processes and a rational base to exploit the protein corona for targeted gene delivery on future in vivo applications. [Mh] Termos MeSH primário: Ciclodextrinas/química DNA/química Coroa de Proteína/química Transfecção/métodos [Mh] Termos MeSH secundário: Biofísica Células HeLa Seres Humanos Células MCF-7 Nanopartículas [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cyclodextrins); 0 (Protein Corona); 9007-49-2 (DNA) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171103 [Lr] Data última revisão: 171103 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170320 [St] Status: MEDLINE

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[PMID]: 28273493 [Au] Autor: Palchetti S; Pozzi D; Capriotti AL; Barbera G; Chiozzi RZ; Digiacomo L; Peruzzi G; Caracciolo G; Laganà A [Ad] Endereço: Department of Molecular Medicine, "Sapienza" University of Rome, Viale Regina Elena 291, 00161, Rome, Italy; Istituti Fisioterapici Ospitalieri, Istituto Regina Elena,Via Elio Chianesi 53, 00144 Rome, Italy. [Ti] Título: Influence of dynamic flow environment on nanoparticle-protein corona: From protein patterns to uptake in cancer cells. [So] Source: Colloids Surf B Biointerfaces;153:263-271, 2017 May 01. [Is] ISSN: 1873-4367 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs' biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines. [Mh] Termos MeSH primário: Citometria de Fluxo Nanopartículas/química Nanopartículas/metabolismo Coroa de Proteína/química Coroa de Proteína/metabolismo [Mh] Termos MeSH secundário: Animais Bovinos Células HeLa Seres Humanos Células MCF-7 Propriedades de Superfície [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Protein Corona) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170417 [Lr] Data última revisão: 170417 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170309 [St] Status: MEDLINE

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[PMID]: 28131092 [Au] Autor: Lai W; Wang Q; Li L; Hu Z; Chen J; Fang Q [Ad] Endereço: Key Laboratory for Biological Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences, Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, P.R. China. [Ti] Título: Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns. [So] Source: Colloids Surf B Biointerfaces;152:317-325, 2017 Apr 01. [Is] ISSN: 1873-4367 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Determining how nanomaterials interact with plasma will assist in understanding their effects on the biological system. This work presents a systematic study of the protein corona formed from human plasma on 20nm silver and gold nanoparticles with three different surface modifications, including positive and negative surface charges. The results show that all nanoparticles, even those with positive surface modifications, acquire negative charges after interacting with plasma. Approximately 300 proteins are identified on the coronas, while 99 are commonly found on each nanomaterial. The 20 most abundant proteins account for over 80% of the total proteins abundance. Remarkably, the surface charge and core of the nanoparticles, as well as the isoelectric point of the plasma proteins, are found to play significant roles in determining the nanoparticle coronas. Albumin and globulins are present at levels of less than 2% on these nanoparticle coronas. Fibrinogen, which presents in the plasma but not in the serum, preferably binds to negatively charged gold nanoparticles. These observations demonstrate the specific plasma protein binding pattern of silver and gold nanoparticles, as well as the importance of the surface charge and core in determining the protein corona compositions. The potential downstream biological impacts of the corona proteins were also investigated. [Mh] Termos MeSH primário: Ouro/química Nanopartículas Metálicas/química Coroa de Proteína/química Prata/química [Mh] Termos MeSH secundário: Seres Humanos Ligação Proteica Propriedades de Superfície [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Protein Corona); 3M4G523W1G (Silver); 7440-57-5 (Gold) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170321 [Lr] Data última revisão: 170321 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170129 [St] Status: MEDLINE

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[PMID]: 28095684 [Au] Autor: de Puig H; Bosch I; Carré-Camps M; Hamad-Schifferli K [Ti] Título: Effect of the Protein Corona on Antibody-Antigen Binding in Nanoparticle Sandwich Immunoassays. [So] Source: Bioconjug Chem;28(1):230-238, 2017 Jan 18. [Is] ISSN: 1520-4812 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: We investigated the effect of the protein corona on the function of nanoparticle (NP) antibody (Ab) conjugates in dipstick sandwich immunoassays. Ab specific for Zika virus nonstructural protein 1 (NS1) were conjugated to gold NPs, and another anti-NS1 Ab was immobilized onto the nitrocellulose membrane. Sandwich immunoassay formation was influenced by whether the strip was run in corona forming conditions, i.e., in human serum. Strips run in buffer or pure solutions of bovine serum albumin exhibited false positives, but those run in human serum did not. Serum pretreatment of the nitrocellulose also eliminated false positives. Corona formation around the NP-Ab in serum was faster than the immunoassay time scale. Langmuir binding analysis determined how the immobilized Ab affinity for the NP-Ab/NS1 was impacted by corona formation conditions, quantified as an effective dissociation constant, K . Results show that corona formation mediates the specificity and sensitivity of the antibody-antigen interaction of Zika biomarkers in immunoassays, and plays a critical but beneficial role. [Mh] Termos MeSH primário: Reações Antígeno-Anticorpo Imunoensaio/métodos Nanopartículas/química Coroa de Proteína/metabolismo [Mh] Termos MeSH secundário: Biomarcadores Seres Humanos Ressonância de Plasmônio de Superfície Zika virus/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Protein Corona) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170705 [Lr] Data última revisão: 170705 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170119 [St] Status: MEDLINE [do] DOI: 10.1021/acs.bioconjchem.6b00523

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[PMID]: 28075572 [Au] Autor: Levak M; Buric P; Dutour Sikiric M; Domazet Jurasin D; Mikac N; Bacic N; Drexel R; Meier F; Jaksic Z; Lyons DM [Ad] Endereço: Ruder Boskovic Institute , Center for Marine Research, G. Paliaga 5, 52210 Rovinj, Croatia. [Ti] Título: Effect of Protein Corona on Silver Nanoparticle Stabilization and Ion Release Kinetics in Artificial Seawater. [So] Source: Environ Sci Technol;51(3):1259-1266, 2017 Feb 07. [Is] ISSN: 1520-5851 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In parallel with the growing use of nanoparticle-containing products, their release into the environment over the coming years is expected to increase significantly. With many large population centers located in near-coastal areas, and increasing evidence that various nanoparticles may be toxic to a range of organisms, biota in estuarine and coastal waters may be particularly vulnerable. While size effects may be important in cases, silver nanoparticles have been found to be toxic in large part due to their release of silver ions. However, there is relatively little data available on how nanoparticle coatings can affect silver ion release in estuarine or marine waters. We have found that albumin, as a model for biocorona-forming macromolecules which nanoparticles may encounter in wastewater streams, stabilizes silver colloids from agglomeration in high salinity marine waters by electrosteric repulsion for long time periods. A minimum mass ratio of about 130 for albumin:silver nanoparticles (40 nm) was required for stable dispersion in seawater. Increasing albumin concentration was also found to reduce dissolution of nanoparticles in seawater with up to 3.3 times lower concentrations of silver ions noted. Persistent colloids and slow sustained ion release may have important consequences for biota in these environmental compartments. [Mh] Termos MeSH primário: Coroa de Proteína Prata [Mh] Termos MeSH secundário: Cinética Nanopartículas Metálicas Água do Mar Poluentes Químicos da Água/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Protein Corona); 0 (Water Pollutants, Chemical); 3M4G523W1G (Silver) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170609 [Lr] Data última revisão: 170609 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170112 [St] Status: MEDLINE [do] DOI: 10.1021/acs.est.6b03161

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[PMID]: 28075126 [Au] Autor: Dai Q; Guo J; Yan Y; Ang CS; Bertleff-Zieschang N; Caruso F [Ad] Endereço: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, Victoria 3010, Australia. [Ti] Título: Cell-Conditioned Protein Coronas on Engineered Particles Influence Immune Responses. [So] Source: Biomacromolecules;18(2):431-439, 2017 Feb 13. [Is] ISSN: 1526-4602 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A protein corona, which forms on engineered particles as soon as they are introduced into biological environments, is known to provide particles with a "biological identity". Protein coronas derived from various biological environments have been demonstrated to alter the cell internalization mechanism, to diminish targeting ability and to induce nanoparticle aggregation. So far, most of these studies have challenged engineered particles with a static biological environment. However, the extracellular environment is highly dynamic due to the process termed "cell-conditioning", in which cells deplete and secrete biomolecules. In this work, we demonstrate that protein coronas formed on engineered particles from such cell-conditioned media affect the biophysical particle properties and protein adsorption differently to protein coronas derived from an unconditioned environment. When investigating particles with protein coronas formed in various biologically relevant environments for their interaction with immune cells, we observed differences in pro-inflammatory cytokine secretion and immune cell apoptosis. We found that the particles either increased or mitigated the secretion of a specific cytokine, depending on the environment where the protein corona was formed. Our study suggests that the use of protein coronas could be useful to engineer drug carriers for elongated circulation, enhanced biocompatibility, and lower toxicity by triggering a specific immune response. [Mh] Termos MeSH primário: Apoptose Macrófagos/imunologia Monócitos/imunologia Nanopartículas/química Coroa de Proteína/química [Mh] Termos MeSH secundário: Células Cultivadas Citocinas/metabolismo Seres Humanos Macrófagos/metabolismo Macrófagos/patologia Monócitos/metabolismo Monócitos/patologia Coroa de Proteína/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cytokines); 0 (Protein Corona) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171011 [Lr] Data última revisão: 171011 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170112 [St] Status: MEDLINE [do] DOI: 10.1021/acs.biomac.6b01545

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