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  1 / 5042 MEDLINE  
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[PMID]:29232573
[Au] Autor:Fatehi D; Mohammadi M; Shekarchi B; Shabani A; Seify M; Rostamzadeh A
[Ad] Endereço:Department of Medical Physics, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
[Ti] Título:Radioprotective effects of Silymarin on the sperm parameters of NMRI mice irradiated with γ-rays.
[So] Source:J Photochem Photobiol B;178:489-495, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Free radicals and reactive oxygen species (ROS) are generated using various endogenous systems or from external sources such as exposure to different physiochemicals. Ionizing radiation damage to the cell can be caused by the direct or indirect effects of radiotherapy processes. Silymarin (SM), a flavanolignan compound, has been identified as a natural potent antioxidant with cytoprotection activities due to scavenging free radicals. The aim of the present study was to evaluate the radioprotective effect of SM on sperm parameters of mice induced by γ-rays. A total number of 40 adult, male NMRI mice were randomly divided into four equal groups. The control group was neither treated with SM nor irradiated by γ-rays. The second group was only irradiated with 2Gy of γ-rays. The third group was firstly treated with 50mg/kg of SM for 7 consecutive days, and one day later, last injections were irradiated by 2Gy of γ-rays. The fourth groups received only 50mg/kg of SM for 7 consecutive days. All the animals were treated intraperitoneally. Histopathological and morphometrical examinations were performed. The data were analyzed using ANOVA and Tukey post hoc test. A value of p<0.05 was considered significant. The results showed that in the radiation-only group when compared with those treated with SM and irradiated, a significant different was observed in testicular parameters and DNA damage (p<0.05). In conclusion, SM can be considered as a promising herbal radioprotective agent in complementary medicine which may play an important role to protect normal spermatocytes against possible effects of γ-radiation-induced cellular damage.
[Mh] Termos MeSH primário: Raios gama
Protetores contra Radiação/farmacologia
Silimarina/farmacologia
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Dano ao DNA/efeitos dos fármacos
Dano ao DNA/efeitos da radiação
Histonas/metabolismo
Masculino
Camundongos
Protaminas/metabolismo
Protetores contra Radiação/química
Espécies Reativas de Oxigênio/metabolismo
Silimarina/química
Motilidade Espermática/efeitos dos fármacos
Motilidade Espermática/efeitos da radiação
Espermatozoides/efeitos da radiação
Testículo/efeitos dos fármacos
Testículo/fisiologia
Testículo/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histones); 0 (Protamines); 0 (Radiation-Protective Agents); 0 (Reactive Oxygen Species); 0 (Silymarin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  2 / 5042 MEDLINE  
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[PMID]:29235322
[Au] Autor:Bobrovnik SA; Demchenko MO; Komisarenko SV
[Ti] Título:Kinetic parameters of polyreactive immunoglobulins interaction with antigens in the presence of protamine.
[So] Source:Ukr Biochem J;88(3):29-35, 2016 May-Jun.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The discovered earlier phenomenon of the enhancment of polyreactive immunoglobulines (PRIGs) binding to antigens in the presence of protamine and Tween 20 was investigated in more details. The comparative analysis of PRIGs reaction dynamics with immobilized antigen was provided. In addition, the rate constants for the reaction and the affinity constants of PRIGs-antigen binding in the presence or absence of optimal protamine concentration were determined. The rate constant of PRIGs-antigen reaction did not increase in the presence of protamine optimal concentration and was even reduced approximately twice. However, in the presence of protamine the concentration of reactive PRIGs molecules, that were able to interact with antigen, increased approximately 30 times, and this led to strong reaction rate increase. Protamine also influenced the affinity constant of PRIGs-antigen binding, which increased approximately three times. The suggestion was made that such protamine effect was due to its influence on the PRIGs molecules special structure, and, as a result of the conformational change PRIGs became able to bind more effectively to the antigens.
[Mh] Termos MeSH primário: Reações Antígeno-Anticorpo
Antígenos/química
Imunoglobulinas/química
Protaminas/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Animais
Afinidade de Anticorpos
Especificidade de Anticorpos
Antígenos/imunologia
Bovinos
Ensaio de Imunoadsorção Enzimática
Proteínas Imobilizadas
Cinética
Camundongos
Polissorbatos/química
Salmão
Soroalbumina Bovina/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Immobilized Proteins); 0 (Immunoglobulins); 0 (Polysorbates); 0 (Protamines); 27432CM55Q (Serum Albumin, Bovine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.03.029


  3 / 5042 MEDLINE  
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[PMID]:29227611
[Au] Autor:Rushchak VV; Chashchyn MO
[Ti] Título:Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs.
[So] Source:Ukr Biochem J;88(2):98-106, 2016 Mar-Apr.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:In this work the experimental metabolic syndrome on the basis of protamine sulfate modeling in guinea pigs was reproduced and pathological processes in the liver of experimental animals were studied. We determined the level of free radicals and markers of liver damage in the blood of experimental animals. We investigated the liver glycogen content and K+,Na+-ATPase activity in the liver of experimental animals as well as measured the cytochrome P450 2E1 (CY P2E1) expression ­ one of the main factors of oxidative stress. Evidence of development of hepatotoxic processes, increasing of the CY P2E1 level as well as of the free radical level in the animals with metabolic syndrome were found. Using of CY P2E1 inhibitors had shown that the free radical level in the blood of experimental animals depended on the level of the enzyme expression and activity. The obtained results suggest that the changes in the CY P2E1 expression play an important role in the development of hepatotoxic processes upon experimental metabolic syndrome. It was assumed that pharmacological correction of the enzyme expression may be an important mechanism for the influence on the metabolic syndrome clinical course.
[Mh] Termos MeSH primário: Inibidores do Citocromo P-450 CYP2E1/farmacologia
Citocromo P-450 CYP2E1/genética
Fígado/efeitos dos fármacos
Síndrome Metabólica/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Citocromo P-450 CYP2E1/metabolismo
Dissulfiram/farmacologia
Radicais Livres/antagonistas & inibidores
Radicais Livres/metabolismo
Regulação da Expressão Gênica
Glicogênio/metabolismo
Cobaias
Fígado/enzimologia
Fígado/patologia
Masculino
Síndrome Metabólica/induzido quimicamente
Síndrome Metabólica/enzimologia
Síndrome Metabólica/patologia
Estresse Oxidativo/efeitos dos fármacos
Protaminas
Pirazóis/farmacologia
Quercetina/farmacologia
ATPase Trocadora de Sódio-Potássio/genética
ATPase Trocadora de Sódio-Potássio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytochrome P-450 CYP2E1 Inhibitors); 0 (Free Radicals); 0 (Protamines); 0 (Pyrazoles); 83LCM6L2BY (fomepizole); 9005-79-2 (Glycogen); 9IKM0I5T1E (Quercetin); EC 1.14.13.- (Cytochrome P-450 CYP2E1); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase); TR3MLJ1UAI (Disulfiram)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.02.098


  4 / 5042 MEDLINE  
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[PMID]:29216327
[Au] Autor:Kuboyama K; Tanga N; Suzuki R; Fujikawa A; Noda M
[Ad] Endereço:Division of Molecular Neurobiology, National Institute for Basic Biology (NIBB), Okazaki, Aichi, Japan.
[Ti] Título:Protamine neutralizes chondroitin sulfate proteoglycan-mediated inhibition of oligodendrocyte differentiation.
[So] Source:PLoS One;12(12):e0189164, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chondroitin sulfate proteoglycans (CSPGs), which are enriched in demyelinating plaques in neurodegenerative diseases, such as multiple sclerosis (MS), impair remyelination by inhibiting the migration and differentiation of oligodendrocyte precursor cells (OPCs) in the central nervous system (CNS). We herein show that protamine (PRM, also known as a heparin antagonist) effectively neutralizes the inhibitory activities of CSPGs, thereby enhancing OPC differentiation and (re)myelination in mice. Cell-based assays using mouse OPC-like OL1 cells revealed that the PRM treatment exerted masking effects on extracellular CSPGs and improved oligodendrocyte differentiation on inhibitory CSPG-coated substrates. PRM also bound to the extracellular region of protein tyrosine phosphatase receptor type Z (PTPRZ), a membrane-spanning CSPG predominantly expressed in OPCs, and functioned as a ligand mimetic of PTPRZ, thereby suppressing its negative regulatory activity on oligodendrocyte differentiation. In primary cultures, the differentiation of OPCs from wild-type and Ptprz-deficient mice was equally enhanced by PRM. Moreover, the intranasal administration of PRM accelerated myelination in the developing mouse brain, and its intracerebroventricular administration stimulated remyelination after cuprizone-induced demyelination. These results indicate that PRM has CSPG-neutralizing activity which promotes oligodendrocyte differentiation under developmental and morbid conditions.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Proteoglicanas de Sulfatos de Condroitina/metabolismo
Oligodendroglia/citologia
Protaminas/farmacologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/citologia
Encéfalo/efeitos dos fármacos
Camundongos
Bainha de Mielina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chondroitin Sulfate Proteoglycans); 0 (Protamines)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189164


  5 / 5042 MEDLINE  
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[PMID]:28712647
[Au] Autor:Khalil A; Parker M; Brown SE; Cevik SE; Guo LW; Jensen J; Olmsted A; Portman D; Wu H; Suvorov A
[Ad] Endereço:Department of Environmental Health Sciences, University of Massachusetts Amherst,686 N. Pleasant St., 149A Goessmann, Amherst, MA, 01003-9303, United States; Medical Biotechnology Department, Genetic Engineering & Biotechnology Research Institute, City of Scientific Research & Technological
[Ti] Título:Perinatal exposure to 2,2',4'4' -Tetrabromodiphenyl ether induces testicular toxicity in adult rats.
[So] Source:Toxicology;389:21-30, 2017 Aug 15.
[Is] ISSN:1879-3185
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Since 1965, polybrominated diphenyl ethers (PBDEs) have been used internationally as flame-retardant additives. PBDEs were recently withdrawn from commerce in North America and Europe due to their environmental persistence, bioaccumulative properties and endocrine-disrupting effects. Generations exposed perinatally to the highest environmental doses of PBDE account for one-fifth of the total United States population. While, toxicity of PBDE for the male reproductive system has been demonstrated in several human and animal studies, the long-lasting effects of perinatal exposures on male reproduction are still poorly understood. In this study, pregnant Wistar rats were exposed to 0.2mg/kg 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) from gestation day 8 until postnatal day 21. Male reproductive outcomes were analyzed on postnatal day 120 in offspring. Exposed animals had significantly smaller testes, displayed decreased sperm production per testis weight, had significantly increased percentage of morphologically abnormal spermatozoa, and showed an increase in spermatozoa head size. Perinatal BDE-47 exposure led to significant changes in testes transcriptome, including suppression of genes essential for spermatogenesis and activation of immune response genes. In particular, we observed a 4-fold average decrease in expression of protamine and transition protein genes in testes, suggesting that histone-protamine exchange may be dysregulated during spermatogenesis, resulting in an aberrant sperm epigenome. The possibility of long-lasting effects of developmental PBDE exposures calls for additional studies to build a foundation for the development of preventive and protective interventions against the environmentally-induced decline in fertility.
[Mh] Termos MeSH primário: Fertilidade/efeitos dos fármacos
Retardadores de Chama/toxicidade
Éteres Difenil Halogenados/toxicidade
Exposição Materna/efeitos adversos
Efeitos Tardios da Exposição Pré-Natal
Espermatogênese/efeitos dos fármacos
Espermatozoides/efeitos dos fármacos
Testículo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Animais Recém-Nascidos
Biologia Computacional
Bases de Dados Genéticas
Epigênese Genética/efeitos dos fármacos
Feminino
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Genoma/efeitos dos fármacos
Idade Gestacional
Masculino
Camundongos
Gravidez
Protaminas/metabolismo
Ratos Wistar
Contagem de Espermatozoides
Cabeça do Espermatozoide/efeitos dos fármacos
Cabeça do Espermatozoide/metabolismo
Cabeça do Espermatozoide/patologia
Motilidade Espermática/efeitos dos fármacos
Espermatozoides/metabolismo
Espermatozoides/patologia
Testículo/metabolismo
Testículo/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flame Retardants); 0 (Halogenated Diphenyl Ethers); 0 (Protamines); 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE


  6 / 5042 MEDLINE  
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[PMID]:28552346
[Au] Autor:Gou LT; Kang JY; Dai P; Wang X; Li F; Zhao S; Zhang M; Hua MM; Lu Y; Zhu Y; Li Z; Chen H; Wu LG; Li D; Fu XD; Li J; Shi HJ; Liu MF
[Ad] Endereço:State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; Departm
[Ti] Título:Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.
[So] Source:Cell;169(6):1090-1104.e13, 2017 Jun 01.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genetic studies have elucidated critical roles of Piwi proteins in germline development in animals, but whether Piwi is an actual disease gene in human infertility remains unknown. We report germline mutations in human Piwi (Hiwi) in patients with azoospermia that prevent its ubiquitination and degradation. By modeling such mutations in Piwi (Miwi) knockin mice, we demonstrate that the genetic defects are directly responsible for male infertility. Mechanistically, we show that MIWI binds the histone ubiquitin ligase RNF8 in a Piwi-interacting RNA (piRNA)-independent manner, and MIWI stabilization sequesters RNF8 in the cytoplasm of late spermatids. The resulting aberrant sperm show histone retention, abnormal morphology, and severely compromised activity, which can be functionally rescued via blocking RNF8-MIWI interaction in spermatids with an RNF8-N peptide. Collectively, our findings identify Piwi as a factor in human infertility and reveal its role in regulating the histone-to-protamine exchange during spermiogenesis.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Proteínas Argonauta/metabolismo
Azoospermia/genética
Mutação
[Mh] Termos MeSH secundário: Animais
Azoospermia/metabolismo
Cromatina/metabolismo
Análise Mutacional de DNA
Proteínas de Ligação a DNA/metabolismo
Modelos Animais de Doenças
Feminino
Técnicas de Introdução de Genes
Histonas/metabolismo
Seres Humanos
Íntrons
Masculino
Camundongos
Linhagem
Protaminas/metabolismo
Proteólise
Espermatogênese
Ubiquitinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (Chromatin); 0 (DNA-Binding Proteins); 0 (Histones); 0 (PIWIL1 protein, human); 0 (Protamines); 0 (RNF8 protein, human)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE


  7 / 5042 MEDLINE  
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[PMID]:28526142
[Au] Autor:Meltzer J; Guenzer JR
[Ad] Endereço:Department of Anesthesiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: Joseph.Guenzer@hsc.utah.edu.
[Ti] Título:Anticoagulant Reversal and Anesthetic Considerations.
[So] Source:Anesthesiol Clin;35(2):191-205, 2017 Jun.
[Is] ISSN:1932-2275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bleeding complications are a common concern with the use of anticoagulant agents. In many situations, reversing of neutralizing their effects may be warranted. Prothrombin complex concentrate replaces coagulation factors lowered by warfarin, as does fresh frozen plasma, but in a more concentrated form. Protamine negates the effect of heparin and combines chemically with heparin molecules to form an inactive salt. It also partially reverses the effects of low-molecular-weight heparin. Recombinant activated factor VII is a nonspecific procoagulant that activates the extrinsic clotting pathway, resulting in thrombin generation, but does not directly neutralize the activity of any of the new oral anticoagulants.
[Mh] Termos MeSH primário: Anticoagulantes
Coagulantes/farmacologia
[Mh] Termos MeSH secundário: Anestésicos
Antitrombinas/farmacocinética
Antitrombinas/farmacologia
Fatores de Coagulação Sanguínea/farmacocinética
Fatores de Coagulação Sanguínea/farmacologia
Coagulantes/farmacocinética
Dabigatrana/farmacocinética
Dabigatrana/farmacologia
Fator VIIa/farmacocinética
Fator VIIa/farmacologia
Inibidores do Fator Xa/farmacocinética
Inibidores do Fator Xa/farmacologia
Hemorragia
Seres Humanos
Protaminas/farmacocinética
Protaminas/farmacologia
Vitamina K/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anesthetics); 0 (Anticoagulants); 0 (Antithrombins); 0 (Blood Coagulation Factors); 0 (Coagulants); 0 (Factor Xa Inhibitors); 0 (Protamines); 12001-79-5 (Vitamin K); 37224-63-8 (prothrombin complex concentrates); EC 3.4.21.21 (Factor VIIa); I0VM4M70GC (Dabigatran)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170521
[St] Status:MEDLINE


  8 / 5042 MEDLINE  
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[PMID]:28412438
[Au] Autor:Simard O; Niavarani SR; Gaudreault V; Boissonneault G
[Ad] Endereço:Department of Biochemistry, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Canada.
[Ti] Título:Torsional stress promotes trinucleotidic expansion in spermatids.
[So] Source:Mutat Res;800-802:1-7, 2017 Aug.
[Is] ISSN:1873-135X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Trinucleotide repeats are involved in various neurodegenerative diseases and are highly unstable both in dividing or non-dividing cells. In Huntington disease (HD), the age of onset of symptoms is inversely correlated to the number of CAG repeats within exon 1 of the HTT gene. HD shows paternal anticipation as CAG repeats are increased during spermatogenesis. CAG expansion were indeed found to be generated during the chromatin remodeling in spermatids where most histones are evicted and replaced by protamines. This process involves striking change in DNA topology since free supercoils must be eliminated. Using an in vitro CAG repeat reporter assay and a highly active nuclear extracts from spermatids, we demonstrate that free negative supercoils result in CAG TNR expansion at a stabilized hairpin. We also suggest a possible role for protamines in promoting localized torsional stress and consequently TNR expansion. The transient increase in torsional stress during spermiogenesis may therefore provide an ideal context for the generation of such secondary DNA structures leading to the paternal anticipation of trinucleotidic diseases.
[Mh] Termos MeSH primário: DNA Super-Helicoidal/genética
Espermátides/metabolismo
Torção Mecânica
Repetições de Trinucleotídeos
[Mh] Termos MeSH secundário: Animais
Canabidiol/análogos & derivados
Canabidiol/farmacologia
Montagem e Desmontagem da Cromatina/genética
DNA Topoisomerases Tipo II/metabolismo
Éxons
Histonas/genética
Histonas/metabolismo
Proteína Huntingtina/genética
Proteína Huntingtina/metabolismo
Doença de Huntington/genética
Masculino
Camundongos
Protaminas/metabolismo
Reprodutibilidade dos Testes
Espermatogênese/genética
Inibidores da Topoisomerase II/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Superhelical); 0 (HTT protein, human); 0 (Histones); 0 (Huntingtin Protein); 0 (Protamines); 0 (Topoisomerase II Inhibitors); 137252-25-6 (cannabidiol hydroxyquinone); 19GBJ60SN5 (Cannabidiol); EC 5.99.1.3 (DNA Topoisomerases, Type II)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170417
[St] Status:MEDLINE


  9 / 5042 MEDLINE  
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[PMID]:28366643
[Au] Autor:Barral S; Morozumi Y; Tanaka H; Montellier E; Govin J; de Dieuleveult M; Charbonnier G; Couté Y; Puthier D; Buchou T; Boussouar F; Urahama T; Fenaille F; Curtet S; Héry P; Fernandez-Nunez N; Shiota H; Gérard M; Rousseaux S; Kurumizaka H; Khochbin S
[Ad] Endereço:CNRS UMR 5309, Inserm U1209, Université Grenoble Alpes, Institute for Advanced Biosciences, Grenoble 38700, France.
[Ti] Título:Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
[So] Source:Mol Cell;66(1):89-101.e8, 2017 Apr 06.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Histone replacement by transition proteins (TPs) and protamines (Prms) constitutes an essential step for the successful production of functional male gametes, yet nothing is known on the underlying functional interplay between histones, TPs, and Prms. Here, by studying spermatogenesis in the absence of a spermatid-specific histone variant, H2A.L.2, we discover a fundamental mechanism involved in the transformation of nucleosomes into nucleoprotamines. H2A.L.2 is synthesized at the same time as TPs and enables their loading onto the nucleosomes. TPs do not displace histones but rather drive the recruitment and processing of Prms, which are themselves responsible for histone eviction. Altogether, the incorporation of H2A.L.2 initiates and orchestrates a series of successive transitional states that ultimately shift to the fully compacted genome of the mature spermatozoa. Hence, the current view of histone-to-nucleoprotamine transition should be revisited and include an additional step with H2A.L.2 assembly prior to the action of TPs and Prms.
[Mh] Termos MeSH primário: Montagem e Desmontagem da Cromatina
Cromatina/metabolismo
Histonas/metabolismo
Nucleossomos/metabolismo
Protaminas/metabolismo
Espermatogênese
Espermatozoides/metabolismo
[Mh] Termos MeSH secundário: Animais
Células COS
Cercopithecus aethiops
Cromatina/genética
Proteínas Cromossômicas não Histona/genética
Proteínas Cromossômicas não Histona/metabolismo
Biologia Computacional
Bases de Dados Genéticas
Fertilidade
Regulação da Expressão Gênica no Desenvolvimento
Predisposição Genética para Doença
Genoma
Histonas/deficiência
Histonas/genética
Infertilidade Masculina/genética
Infertilidade Masculina/metabolismo
Infertilidade Masculina/patologia
Infertilidade Masculina/fisiopatologia
Masculino
Camundongos da Linhagem 129
Camundongos Knockout
Nucleossomos/genética
Fenótipo
Espermatogênese/genética
Espermatozoides/patologia
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Chromosomal Proteins, Non-Histone); 0 (Histones); 0 (Nucleosomes); 0 (Protamines); 0 (deoxyribonucleoprotamine); 0 (spermatid transition proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


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[PMID]:28356290
[Au] Autor:Tyagi P; Janicki JJ; Hitchens TK; Foley LM; Kashyap M; Yoshimura N; Kaufman J
[Ad] Endereço:Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; tyagip@upmc.edu.
[Ti] Título:Novel contrast mixture improves bladder wall contrast for visualizing bladder injury.
[So] Source:Am J Physiol Renal Physiol;313(2):F155-F162, 2017 Aug 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here, we tested whether combined contrast-enhanced magnetic resonance imaging (CCE-MRI), using a mixture of gadolinium- and iron oxide-based contrast agents, can segment the bladder wall from the bladder lumen. CCE-MRI relies on the differences in particle size and contrast mechanisms of two agents for improved image contrast. Under isoflurane anesthesia, T1-weighted imaging of adult female Sprague-Dawley rat bladder was performed using standard turbospin echo sequences at 7 Tesla, before and after transurethral instillation of 0.3 ml of single-contrast MRI or CCE-MRI composed of 0.4-64 mM of gadolinium chelate (Gd-DTPA/Gadavist) and 5 mM ferumoxytol. Bladder wall contrast was assessed in the control group exposed to saline and in the bladder injury group exposed to 0.5 ml of protamine sulfate (10 mg/ml) for 30 min. CCE-MRI following instillation of 0.4-4 mM Gd-DTPA and 5 mM ferumoxytol mixture achieved segmentation between the bladder lumen and bladder wall. Hyperintensity in the bladder wall combined with hypointensity in the lumen is consistent with the increased diffusion of the dissolved Gd-DTPA and simultaneous localization of the larger nanoparticles of ferumoxytol in the lumen. The normalized hyperintense signal in the bladder wall increased from 0.46 ± 0.07 in control group to 0.73 ± 0.14 in the protamine sulfate-exposed group ( < 0.0001). CCE-MRI following instillation of contrast mixture identifies bladder wall changes likely associated with bladder injury with improved image contrast.
[Mh] Termos MeSH primário: Meios de Contraste/administração & dosagem
Óxido Ferroso-Férrico/administração & dosagem
Imagem por Ressonância Magnética/métodos
Compostos Organometálicos/administração & dosagem
Doenças da Bexiga Urinária/diagnóstico por imagem
Bexiga Urinária/diagnóstico por imagem
[Mh] Termos MeSH secundário: Administração Intravesical
Animais
Modelos Animais de Doenças
Feminino
Nanopartículas
Tamanho da Partícula
Valor Preditivo dos Testes
Protaminas
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Fatores de Tempo
Bexiga Urinária/patologia
Doenças da Bexiga Urinária/induzido quimicamente
Doenças da Bexiga Urinária/patologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 0 (Organometallic Compounds); 0 (Protamines); 1BJ477IO2L (gadobutrol); XM0M87F357 (Ferrosoferric Oxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00609.2016



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