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Pesquisa : D12.776.765.675 [Categoria DeCS]
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[PMID]:28467358
[Au] Autor:Qu Y; Liu S; Bao W; Xue X; Ma Z; Yokawa K; Baluska F; Wan Y
[Ad] Endereço:College of Biological Sciences and Biotechnology, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing 100083, China. quyanli@bjfu.edu.cn.
[Ti] Título:Expression of Root Genes in Arabidopsis Seedlings Grown by Standard and Improved Growing Methods.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Roots of seedlings grown in the laboratory using the traditional plant-growing culture system (TPG) were covered to maintain them in darkness. This new method is based on a dark chamber and is named the improved plant-growing method (IPG). We measured the light conditions in dark chambers, and found that the highest light intensity was dramatically reduced deeper in the dark chamber. In the bottom and side parts of dark chambers, roots were almost completely shaded. Using the high-throughput RNA sequencing method on the whole RNA extraction from roots, we compared the global gene expression levels in roots of seedlings from these two conditions and identified 141 differently expressed genes (DEGs) between them. According to the KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment, the flavone and flavonol biosynthesis and flavonoid biosynthesis pathways were most affected among all annotated pathways. Surprisingly, no genes of known plant photoreceptors were identified as DEGs by this method. Considering that the light intensity was decreased in the IPG system, we collected four sections (1.5 cm for each) of roots grown in TPG and IPG conditions, and the spatial-related differential gene expression levels of plant photoreceptors and polar auxin transporters, including , , , , , , and were analyzed by qRT-PCR. Using these results, we generated a map of the spatial-related expression patterns of these genes under IPG and TPG conditions. The expression levels of light-related genes in roots is highly sensitive to illumination and it provides a background reference for selecting an improved culture method for laboratory-maintained seedlings.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/genética
[Mh] Termos MeSH secundário: Arabidopsis/efeitos da radiação
Escuridão
Flavonas/genética
Flavonoides/genética
Regulação da Expressão Gênica de Plantas/efeitos da radiação
Genes de Plantas
Sequenciamento de Nucleotídeos em Larga Escala
Luz
Fotorreceptores de Plantas/genética
Fitocromo/genética
Raízes de Plantas/efeitos da radiação
RNA/genética
Plântulas/genética
Plântulas/crescimento & desenvolvimento
Transcriptoma/genética
Transcriptoma/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Flavones); 0 (Flavonoids); 0 (Photoreceptors, Plant); 11121-56-5 (Phytochrome); 63231-63-0 (RNA); S2V45N7G3B (flavone); ZTG9LSS5QH (3-hydroxyflavone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  2 / 1603 MEDLINE  
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[PMID]:29236780
[Au] Autor:Wang CC; Sulli M; Fu DQ
[Ad] Endereço:Fruit Biology Laboratory, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
[Ti] Título:The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves.
[So] Source:PLoS One;12(12):e0189481, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycoalkaloids are toxic compounds that are synthesized by many Solanum species. Glycoalkaloid biosynthesis is influenced by plant genetic and environmental conditions. Although many studies have shown that light is an important factor affecting glycoalkaloid biosynthesis, the specific mechanism is currently unknown. Chlorophyll and carotenoid biosynthesis depend on light signal transduction and share some intermediate metabolites with the glycoalkaloid biosynthetic pathway. Here, we used virus-induced gene silencing to silence genes encoding phytoene desaturase (PDS) and magnesium chelatase (CHLI and CHLH) to reduce chlorophyll and carotenoid levels in eggplant leaves. Quantification of carotenoid and chlorophyll levels is analyzed by LC/PDA/APCI/MS and semipolar metabolite profiling by LC/HESI/MS. Notably, the resulting lines showed decreases in glycoalkaloid production. We further found that the expression of some genes involved in the production of glycoalkaloids and other metabolites were suppressed in these silenced lines. Our results indicate that photosynthetic pigment accumulation affects steroidal glycoalkaloid biosynthesis in eggplant leaves. This finding lays the foundation for reducing the levels of endogenous antinutritional compounds in crops.
[Mh] Termos MeSH primário: Alcaloides/biossíntese
Oxirredutases/metabolismo
Fitocromo/metabolismo
Folhas de Planta/metabolismo
Solanum melongena/metabolismo
Esteróis/biossíntese
[Mh] Termos MeSH secundário: Cromatografia Líquida
Espectrometria de Massas
Solanum melongena/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Sterols); 11121-56-5 (Phytochrome); EC 1.- (Oxidoreductases); EC 1.14.99.- (phytoene dehydrogenase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189481


  3 / 1603 MEDLINE  
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[PMID]:28461270
[Au] Autor:Aguilar-Hernández V; Kim DY; Stankey RJ; Scalf M; Smith LM; Vierstra RD
[Ad] Endereço:Department of Biology, Washington University in St. Louis, Campus Box 1137, One Brookings Drive, St. Louis, MO 63130, USA; Department of Genetics, 425-G Henry Mall, University of Wisconsin-Madison, Madison, WI 53706, USA.
[Ti] Título:Mass Spectrometric Analyses Reveal a Central Role for Ubiquitylation in Remodeling the Arabidopsis Proteome during Photomorphogenesis.
[So] Source:Mol Plant;10(6):846-865, 2017 Jun 05.
[Is] ISSN:1752-9867
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The switch from skotomorphogenesis to photomorphogenesis is a key developmental transition in the life of seed plants. While much of the underpinning proteome remodeling is driven by light-induced changes in gene expression, the proteolytic removal of specific proteins by the ubiquitin-26S proteasome system is also likely paramount. Through mass spectrometric analysis of ubiquitylated proteins affinity-purified from etiolated Arabidopsis seedlings before and after red-light irradiation, we identified a number of influential proteins whose ubiquitylation status is modified during this switch. We observed a substantial enrichment for proteins involved in auxin, abscisic acid, ethylene, and brassinosteroid signaling, peroxisome function, disease resistance, protein phosphorylation and light perception, including the phytochrome (Phy) A and phototropin photoreceptors. Soon after red-light treatment, PhyA becomes the dominant ubiquitylated species, with ubiquitin attachment sites mapped to six lysines. A PhyA mutant protected from ubiquitin addition at these sites is substantially more stable in planta upon photoconversion to Pfr and is hyperactive in driving photomorphogenesis. However, light still stimulates ubiquitylation and degradation of this mutant, implying that other attachment sites and/or proteolytic pathways exist. Collectively, we expand the catalog of ubiquitylation targets in Arabidopsis and show that this post-translational modification is central to the rewiring of plants for photoautotrophic growth.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Regulação da Expressão Gênica de Plantas/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Fitocromo/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Plantas Geneticamente Modificadas/metabolismo
Complexo de Endopeptidases do Proteassoma/genética
Complexo de Endopeptidases do Proteassoma/metabolismo
Proteoma/genética
Proteoma/fisiologia
Transdução de Sinais/genética
Transdução de Sinais/fisiologia
Ubiquitinação/genética
Ubiquitinação/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Proteome); 11121-56-5 (Phytochrome); EC 3.4.25.1 (Proteasome Endopeptidase Complex); EC 3.4.99.- (ATP dependent 26S protease)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  4 / 1603 MEDLINE  
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[PMID]:29190504
[Au] Autor:Yoodee S; Kobayashi Y; Songnuan W; Boonchird C; Thitamadee S; Kobayashi I; Narangajavana J
[Ad] Endereço:Department of Biotechnology, Faculty of Science, Mahidol University, Phayathai, Bangkok, Thailand.
[Ti] Título:Phytohormone priming elevates the accumulation of defense-related gene transcripts and enhances bacterial blight disease resistance in cassava.
[So] Source:Plant Physiol Biochem;122:65-77, 2018 Jan.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Cassava bacterial blight (CBB) disease caused by Xanthomonas axonopodis pv. manihotis (Xam) is a severe disease in cassava worldwide. In addition to causing significant cassava yield loss, CBB disease has not been extensively studied, especially in terms of CBB resistance genes. The present research demonstrated the molecular mechanisms underlining the defense response during Xam infection in two cassava cultivars exhibiting different degrees of disease resistance, Huay Bong60 (HB60) and Hanatee (HN). Based on gene expression analysis, ten of twelve putative defense-related genes including, leucine-rich repeat receptor-like kinases (LRR-RLKs), resistance (R), WRKY and pathogenesis-related (PR) genes, were differentially expressed between these two cassava cultivars during Xam infection. The up-regulation of defense-related genes observed in HB60 may be the mechanism required for the reduction of disease severity in the resistant cultivar. Interestingly, priming with salicylic acid (SA) or methyl jasmonate (MeJA) for 24 h before Xam inoculation could enhance the defense response in both cassava cultivars. The disease severity was decreased 10% in the resistant cultivar (HB60) and was remarkably reduced 21% in the susceptible cultivar (HN) by SA/MeJA priming. Priming with Xam inoculation modulated cassava4.1_013417, cassava4.1_030866 and cassava4.1_020555 (highest similarity to MeWRKY59, MePR1 and AtPDF2.2, respectively) expression and led to enhanced resistance of the susceptible cultivar in the second infection. The putative cis-regulatory elements were predicted in an upstream region of these three defense-related genes. The different gene expression levels in these genes between the two cultivars were due to the differences in cis-regulatory elements in their promoter regions. Taken together, our study strongly suggested that the induction of defense-related genes correlated with defense resistance against Xam infection, and exogenous application of SA or MeJA could elevate the defense response in both cultivars of cassava. This finding should pave the way for management to reduce yield loss from disease and genetic improvement in cassava.
[Mh] Termos MeSH primário: Resistência à Doença/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Manihot
Fitocromo/farmacologia
Doenças das Plantas/microbiologia
Transcrição Genética/efeitos dos fármacos
Xanthomonas axonopodis/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Manihot/metabolismo
Manihot/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
11121-56-5 (Phytochrome)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  5 / 1603 MEDLINE  
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[PMID]:27776497
[Au] Autor:Kushanov FN; Pepper AE; Yu JZ; Buriev ZT; Shermatov SE; Saha S; Ulloa M; Jenkins JN; Abdukarimov A; Abdurakhmonov IY
[Ad] Endereço:Center of Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan, University Street-2, Qibray region, Tashkent District, 111215, Uzbekistan.
[Ti] Título:Development, genetic mapping and QTL association of cotton PHYA, PHYB, and HY5-specific CAPS and dCAPS markers.
[So] Source:BMC Genet;17(1):141, 2016 10 24.
[Is] ISSN:1471-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Among SNP markers that become increasingly valuable in molecular breeding of crop plants are the CAPS and dCAPS markers derived from the genes of interest. To date, the number of such gene-based markers is small in polyploid crop plants such as allotetraploid cotton that has A- and D-sub-genomes. The objective of this study was to develop and map new CAPS and dCAPS markers for cotton developmental-regulatory genes that are important in plant breeding programs. RESULTS: Gossypium hirsutum and G. barbadense, are the two cultivated allotetraploid cotton species. These have distinct fiber quality and other agronomic traits. Using comparative sequence analysis of characterized GSTs of the PHYA1, PHYB, and HY5 genes of G. hirsutum and G. barbadense one PHYA1-specific Mbo I/Dpn II CAPS, one PHYB-specific Alu I dCAPS, and one HY5-specific Hinf I dCAPS cotton markers were developed. These markers have successfully differentiated the two allotetraploid genomes (AD and AD ) when tested in parental genotypes of 'Texas Marker-1' ('TM-1'), 'Pima 3-79' and their F hybrids. The genetic mapping and chromosome substitution line-based deletion analyses revealed that PHYA1 gene is located in A-sub-genome chromosome 11, PHYB gene is in A-sub-genome chromosome 10, and HY5 gene is in D-sub-genome chromosome 24, on the reference 'TM-1' x 'Pima 3-79' RIL genetic map. Further, it was found that genetic linkage map regions containing phytochrome and HY5-specific markers were associated with major fiber quality and flowering time traits in previously published QTL mapping studies. CONCLUSION: This study detailed the genome mapping of three cotton phytochrome genes with newly developed CAPS and dCAPS markers. The proximity of these loci to fiber quality and other cotton QTL was demonstrated in two A-subgenome and one D-subgenome chromosomes. These candidate gene markers will be valuable for marker-assisted selection (MAS) programs to rapidly introgress G. barbadense phytochromes and/or HY5 gene (s) into G. hirsutum cotton genotypes or vice versa.
[Mh] Termos MeSH primário: Mapeamento Cromossômico
Genes de Plantas
Genoma de Planta
Genômica
Gossypium/genética
Locos de Características Quantitativas
[Mh] Termos MeSH secundário: Ligação Genética
Marcadores Genéticos
Genômica/métodos
Gossypium/metabolismo
Fitocromo
Característica Quantitativa Herdável
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Genetic Markers); 11121-56-5 (Phytochrome)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  6 / 1603 MEDLINE  
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[PMID]:28401765
[Au] Autor:Chernov KG; Redchuk TA; Omelina ES; Verkhusha VV
[Ad] Endereço:Department of Biochemistry and Developmental Biology, Faculty of Medicine, University of Helsinki , Helsinki 00290, Finland.
[Ti] Título:Near-Infrared Fluorescent Proteins, Biosensors, and Optogenetic Tools Engineered from Phytochromes.
[So] Source:Chem Rev;117(9):6423-6446, 2017 May 10.
[Is] ISSN:1520-6890
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phytochrome photoreceptors absorb far-red and near-infrared (NIR) light and regulate light responses in plants, fungi, and bacteria. Their multidomain structure and autocatalytic incorporation of linear tetrapyrrole chromophores make phytochromes attractive molecular templates for the development of light-sensing probes. A subclass of bacterial phytochromes (BphPs) utilizes heme-derived biliverdin tetrapyrrole, which is ubiquitous in mammalian tissues, as a chromophore. Because biliverdin possesses the largest electron-conjugated chromophore system among linear tetrapyrroles, BphPs exhibit the most NIR-shifted spectra that reside within the NIR tissue transparency window. Here we analyze phytochrome structure and photochemistry to describe the molecular mechanisms by which they function. We then present strategies to engineer BphP-based NIR fluorescent proteins and review their properties and applications in modern imaging technologies. We next summarize designs of reporters and biosensors and describe their use in the detection of protein-protein interactions, proteolytic activities, and posttranslational modifications. Finally, we provide an overview of optogenetic tools developed from phytochromes and describe their use in light-controlled cell signaling, gene expression, and protein localization. Our review provides guidelines for the selection of NIR probes and tools for noninvasive imaging, sensing, and light-manipulation applications, specifically focusing on probes developed for use in mammalian cells and in vivo.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Raios Infravermelhos
Proteínas Luminescentes/genética
Optogenética/métodos
Fitocromo/genética
Engenharia de Proteínas/métodos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Proteínas Luminescentes/química
Proteínas Luminescentes/metabolismo
Fitocromo/química
Fitocromo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Luminescent Proteins); 11121-56-5 (Phytochrome)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.1021/acs.chemrev.6b00700


  7 / 1603 MEDLINE  
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[PMID]:28346403
[Au] Autor:Redchuk TA; Omelina ES; Chernov KG; Verkhusha VV
[Ad] Endereço:Department of Biochemistry and Developmental Biology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
[Ti] Título:Near-infrared optogenetic pair for protein regulation and spectral multiplexing.
[So] Source:Nat Chem Biol;13(6):633-639, 2017 Jun.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Multifunctional optogenetic systems are in high demand for use in basic and biomedical research. Near-infrared-light-inducible binding of bacterial phytochrome BphP1 to its natural PpsR2 partner is beneficial for simultaneous use with blue-light-activatable tools. However, applications of the BphP1-PpsR2 pair are limited by the large size, multidomain structure and oligomeric behavior of PpsR2. Here, we engineered a single-domain BphP1 binding partner, Q-PAS1, which is three-fold smaller and lacks oligomerization. We exploited a helix-PAS fold of Q-PAS1 to develop several near-infrared-light-controllable transcription regulation systems, enabling either 40-fold activation or inhibition. The light-induced BphP1-Q-PAS1 interaction allowed modification of the chromatin epigenetic state. Multiplexing the BphP1-Q-PAS1 pair with a blue-light-activatable LOV-domain-based system demonstrated their negligible spectral crosstalk. By integrating the Q-PAS1 and LOV domains in a single optogenetic tool, we achieved tridirectional protein targeting, independently controlled by near-infrared and blue light, thus demonstrating the superiority of Q-PAS1 for spectral multiplexing and engineering of multicomponent systems.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Regulação da Expressão Gênica
Luz
Complexos Multienzimáticos/química
Optogenética
Monoéster Fosfórico Hidrolases/química
Proteínas Quinases/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Sítios de Ligação
Bioensaio
Epigênese Genética/genética
Citometria de Fluxo
Deleção de Genes
Células HeLa
Seres Humanos
Complexos Multienzimáticos/genética
Complexos Multienzimáticos/metabolismo
Monoéster Fosfórico Hidrolases/genética
Monoéster Fosfórico Hidrolases/metabolismo
Fitocromo/metabolismo
Engenharia de Proteínas
Proteínas Quinases/genética
Proteínas Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Multienzyme Complexes); 11121-56-5 (Phytochrome); EC 2.7.- (Protein Kinases); EC 2.7.11.33 (DUF299 protein, E coli); EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2343


  8 / 1603 MEDLINE  
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[PMID]:28289094
[Au] Autor:Kacprzak S; Njimona I; Renz A; Feng J; Reijerse E; Lubitz W; Krauss N; Scheerer P; Nagano S; Lamparter T; Weber S
[Ad] Endereço:From the Albert-Ludwigs-Universität Freiburg, Institut für Physikalische Chemie, 79104 Freiburg, Germany, Sylwia.Kacprzak@physchem.uni-freiburg.de.
[Ti] Título:Intersubunit distances in full-length, dimeric, bacterial phytochrome Agp1, as measured by pulsed electron-electron double resonance (PELDOR) between different spin label positions, remain unchanged upon photoconversion.
[So] Source:J Biol Chem;292(18):7598-7606, 2017 May 05.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial phytochromes are dimeric light-regulated histidine kinases that convert red light into signaling events. Light absorption by the N-terminal photosensory core module (PCM) causes the proteins to switch between two spectrally distinct forms, Pr and Pfr, thus resulting in a conformational change that modulates the C-terminal histidine kinase region. To provide further insights into structural details of photoactivation, we investigated the full-length Agp1 bacteriophytochrome from the soil bacterium using a combined spectroscopic and modeling approach. We generated seven mutants suitable for spin labeling to enable application of pulsed EPR techniques. The distances between attached spin labels were measured using pulsed electron-electron double resonance spectroscopy to probe the arrangement of the subunits within the dimer. We found very good agreement of experimental and calculated distances for the histidine-kinase region when both subunits are in a parallel orientation. However, experimental distance distributions surprisingly showed only limited agreement with either parallel- or antiparallel-arranged dimer structures when spin labels were placed into the PCM region. This observation indicates that the arrangements of the PCM subunits in the full-length protein dimer in solution differ significantly from that in the PCM crystals. The pulsed electron-electron double resonance data presented here revealed either no or only minor changes of distance distributions upon Pr-to-Pfr photoconversion.
[Mh] Termos MeSH primário: Agrobacterium/química
Proteínas de Bactérias/química
Fitocromo/química
Multimerização Proteica
[Mh] Termos MeSH secundário: Agrobacterium/genética
Agrobacterium/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Espectroscopia de Ressonância de Spin Eletrônica
Mutação
Fitocromo/genética
Fitocromo/metabolismo
Estrutura Quaternária de Proteína
Marcadores de Spin
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Spin Labels); 11121-56-5 (Phytochrome)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.761882


  9 / 1603 MEDLINE  
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[PMID]:28250468
[Au] Autor:Fuller FD; Gul S; Chatterjee R; Burgie ES; Young ID; Lebrette H; Srinivas V; Brewster AS; Michels-Clark T; Clinger JA; Andi B; Ibrahim M; Pastor E; de Lichtenberg C; Hussein R; Pollock CJ; Zhang M; Stan CA; Kroll T; Fransson T; Weninger C; Kubin M; Aller P; Lassalle L; Bräuer P; Miller MD; Amin M; Koroidov S; Roessler CG; Allaire M; Sierra RG; Docker PT; Glownia JM; Nelson S; Koglin JE; Zhu D; Chollet M; Song S; Lemke H; Liang M; Sokaras D; Alonso-Mori R; Zouni A; Messinger J; Bergmann U; Boal AK; Bollinger JM; Krebs C; Högbom M; Phillips GN
[Ad] Endereço:Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
[Ti] Título:Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers.
[So] Source:Nat Methods;14(4):443-449, 2017 Apr.
[Is] ISSN:1548-7105
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
[Mh] Termos MeSH primário: Cristalografia por Raios X/métodos
Lasers
[Mh] Termos MeSH secundário: Acústica
Complexo de Proteína do Fotossistema II/química
Fitocromo/química
Ribonucleotídeo Redutases/química
Espectrometria por Raios X/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosystem II Protein Complex); 11121-56-5 (Phytochrome); EC 1.17.4.- (Ribonucleotide Reductases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1038/nmeth.4195


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[PMID]:28232584
[Au] Autor:Nevarez PA; Qiu Y; Inoue H; Yoo CY; Benfey PN; Schnell DJ; Chen M
[Ad] Endereço:Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, California 92521 (Y.Q., C.Y., M.C.).
[Ti] Título:Mechanism of Dual Targeting of the Phytochrome Signaling Component HEMERA/pTAC12 to Plastids and the Nucleus.
[So] Source:Plant Physiol;173(4):1953-1966, 2017 Apr.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HEMERA (HMR) is a nuclear and plastidial dual-targeted protein. While it functions in the nucleus as a transcriptional coactivator in phytochrome signaling to regulate a distinct set of light-responsive, growth-relevant genes, in plastids it is known as pTAC12, which associates with the plastid-encoded RNA polymerase, and is essential for inducing the plastomic photosynthetic genes and initiating chloroplast biogenesis. However, the mechanism of targeting HMR to the nucleus and plastids is still poorly understood. Here, we show that HMR can be directly imported into chloroplasts through a transit peptide residing in the N-terminal 50 amino acids. Upon cleavage of the transit peptide and additional proteolytic processing, mature HMR, which begins from Lys-58, retains its biochemical properties in phytochrome signaling. Unexpectedly, expression of mature HMR failed to rescue not only the plastidial but also the nuclear defects of the mutant. This is because the predicted nuclear localization signals of HMR are nonfunctional, and therefore mature HMR is unable to accumulate in either plastids or the nucleus. Surprisingly, fusing the transit peptide of the small subunit of Rubisco with mature HMR rescues both its plastidial and nuclear localization and functions. These results, combined with the observation that the nuclear form of HMR has the same reduced molecular mass as plastidial HMR, support a retrograde protein translocation mechanism in which HMR is targeted first to plastids, processed to the mature form, and then relocated to the nucleus.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Núcleo Celular/genética
Plastídeos/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Núcleo Celular/metabolismo
Cloroplastos/genética
Cloroplastos/metabolismo
Regulação da Expressão Gênica de Plantas
Immunoblotting
Microscopia Confocal
Mutação
Fitocromo/genética
Plantas Geneticamente Modificadas
Plastídeos/metabolismo
Transporte Proteico/genética
Proteólise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ribulose-Bifosfato Carboxilase/genética
Ribulose-Bifosfato Carboxilase/metabolismo
Transdução de Sinais/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (HMR protein, Arabidopsis); 0 (Transcription Factors); 11121-56-5 (Phytochrome); EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.00116



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