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[PMID]:29244833
[Au] Autor:Cevallos CG; Jones LR; Pando MA; Carr JK; Avila MM; Quarleri J
[Ad] Endereço:Instituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
[Ti] Título:Genomic characterization and molecular evolution analysis of subtype B and BF recombinant HIV-1 strains among Argentinean men who have sex with men reveal a complex scenario.
[So] Source:PLoS One;12(12):e0189705, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Currently, data on HIV-1 circulating strains among men who have sex with men (MSM) in Argentina is scarce. In South America, the distribution and the prevalence of BF recombinants are dissimilar and exhibit an underappreciated heterogeneity of recombinant structures. Here, we studied for the first time the genetic diversity of HIV-1 BF recombinants and their evolution over time through in-depth phylogenetic analysis and multiple recombination detection methods involving 337 HIV-1 nucleotide sequences (25 near full-length (NFL) and 312 partial pol gene) obtained from Argentinean MSM. The recombination profiles were studied using multiple in silico tools to characterize the genetic mosaicism, and phylogenetic approaches to infer their relationships. The evolutionary history of BF recombinants and subtype B sequences was reconstructed by a Bayesian coalescent-based method. By phylogenetic inference, 81/312 pol sequences clustered within BF clade. Of them, 46 sequences showed a genetic mosaic with CRF12_BF-like patterns, including plausible second-generation recombinants. Other CRFs_BF like (CRF17, 28, 29, 39, 42, 44, 47) and probable URFs_BF were less frequently found. Phylogenetic and recombination analyses on NFL sequences allowed a meticulous definition of new BF mosaics of genomic patterns. The Bayesian analyses pointed out quite consistent onset dates for the CRFs_BF clade based on B and F gene datasets (~1986 and ~1991 respectively). These results indicate that the CRFs_BF variants have been circulating among Argentinean MSM for about 30 years. This study reveals, through growing evidence showing the importance of MSM in the dynamics of the HIV-1 epidemic in Argentina, the coexistence of CRF12_BF-like and high diversity of strains exhibiting several BF mosaic patterns, including non-reported URFs that may reflect active clusters as potential intervention targets to hinder HIV-1 transmission.
[Mh] Termos MeSH primário: Variação Genética
Infecções por HIV/genética
HIV-1/genética
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Mh] Termos MeSH secundário: Adulto
Argentina
Evolução Molecular
Genoma Viral/genética
Infecções por HIV/epidemiologia
Infecções por HIV/transmissão
Infecções por HIV/virologia
HIV-1/patogenicidade
Seres Humanos
Masculino
Filogenia
Minorias Sexuais e de Gênero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189705


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[PMID]:28750322
[Au] Autor:Vancoillie L; Hebberecht L; Dauwe K; Demecheleer E; Dinakis S; Vaneechoutte D; Mortier V; Verhofstede C
[Ad] Endereço:Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
[Ti] Título:Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus.
[So] Source:Virology;510:185-193, 2017 Oct.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HIV-infected patients on antiretroviral therapy (ART) may present low-level viremia (LLV) above the detection level of current viral load assays. In many cases LLV is persistent but does not result in overt treatment failure or selection of drug resistant viral variants. To elucidate whether LLV reflects active virus replication, we extensively sequenced pol and env genes of the viral populations present before and during LLV in 18 patients and searched for indications of genetic evolution. Maximum likelihood phylogenetic trees were inspected for temporal structure both visually and by linear regression analysis of root-to-tip and pairwise distances. Viral coreceptor tropism was assessed at different time points before and during LLV. In none of the patients consistent indications for genetic evolution were found over a median period of 4.8 years of LLV. As such these findings could not provide evidence that active virus replication is the main driver of LLV.
[Mh] Termos MeSH primário: Antirretrovirais/uso terapêutico
Evolução Molecular
Infecções por HIV/tratamento farmacológico
Infecções por HIV/virologia
HIV-1/genética
[Mh] Termos MeSH secundário: HIV-1/isolamento & purificação
HIV-1/fisiologia
Seres Humanos
Estudos Longitudinais
Filogenia
Análise de Sequência de DNA
Tropismo Viral
Produtos do Gene env do Vírus da Imunodeficiência Humana/genética
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Retroviral Agents); 0 (env Gene Products, Human Immunodeficiency Virus); 0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


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[PMID]:28693084
[Au] Autor:Sun XG; Yu HY; Su SL; Lin B; Li JH; Lin L; Tao XR; Qian YS; Kang DM; Xing H
[Ad] Endereço:Shandong Center for Disease Control and Prevention, Jinan 250014, China.
[Ti] Título:[Survey of HIV-1 drug resistance threshold in Shandong Province in 2013-2015].
[So] Source:Zhonghua Yu Fang Yi Xue Za Zhi;51(7):604-609, 2017 Jul 06.
[Is] ISSN:0253-9624
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To survey the prevalence of drug resistant HIV-1 in Shandong province in 2013-2015. WHO truncated sequential sampling technique was adopted by using 77 and 53 samples of newly diagnosed as HIV-1 positive and aged 16-25 years in Shandong province in 2013 and 2015. RNA was prepared and HIV-1 region was amplified by RT-PCR and nested PCR. Pol genetic mutation associated with drug resistance was analyzed. The success rates for sequence acquisition of the survey were 100% (77/77) and 94% (50/53) in 2013 and 2015, and the main subtype was CRF01_AE. A total of 2 surveillance drug-resistance mutation(SDRMs) and 3 SDRMs were found by analyzing the 47 sequences each year, sampled in 2013 and 2015, indicating that the prevalence of drug resistant HIV-1 stains was low in 2013, and moderate in 2015. A total of 5 individuals with drug resistant HIV-1 stains found in this study were mainly infected by homosexual transmission (3 cases), and the other two samples were different: one was infected by heterosexual transmission, the other was infected by IDU. The subtype was CRF01_AE (2 cases) , CRF07_BC (2 cases) and B (1 case) . SDRMs for protease inhibitor (PIs), nucleotide HIV-reverse transcriptase inhibitor (NRTIs) and non-NRTI (NNRTIs) were all found in the individuals with drug resistant HIV-1 stains. CRF01_AE were the main HIV-1 subtypes of recently reported HIV-infected individuals in Shandong province, and the HIV-1 drug resistant strains transmission was catalogued as at low and moderate prevalence level in 2013 and 2015.
[Mh] Termos MeSH primário: Farmacorresistência Viral
Infecções por HIV/tratamento farmacológico
HIV-1/efeitos dos fármacos
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Feminino
Genes pol
Infecções por HIV/sangue
Infecções por HIV/epidemiologia
HIV-1/genética
Seres Humanos
Masculino
Mutação
Reação em Cadeia da Polimerase
Prevalência
RNA Viral/sangue
RNA Viral/genética
Inibidores da Transcriptase Reversa/farmacologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Inquéritos e Questionários
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Reverse Transcriptase Inhibitors); 0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0253-9624.2017.07.006


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[PMID]:28647987
[Au] Autor:Han ZG; Wu H; Liang CY; Gao K; Mai HX; Cai YS; Xu HF
[Ad] Endereço:Department of Operational Control, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
[Ti] Título:[Distribution of HIV-1 subtypes among foreign patients, in Guangzhou, between 2008 and 2010, and in 2015].
[So] Source:Zhonghua Liu Xing Bing Xue Za Zhi;38(6):805-809, 2017 Jun 10.
[Is] ISSN:0254-6450
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To understand the characteristic of subtype distribution among foreigners who were living with HIV-1, in Guangzhou. HIV-1 RNAs were extracted from 114 serum specimens in foreigners diagnosed with HIV-1 infections between 2008 and 2010, and in 2015. Partial gene of HIV-1 genome from these RNA samples were amplified by nested reverse transcription polymerase chain reaction (nested-PCR) with nucleotide sequenced. Subsequently, phylogenetic tree was reconstructed using the sequences of samples and references. Among all the 114 samples, 57.9 were from males and 42.1 from females, with an average age as 35.21 years old and the standard deviation as 9.63 years. A total of 6.8 of the samples were from Africans. The top three subtypes were identified as CRF02_AG, subtype G and subtype C, accounted for 30.7 , 14.9 and 12.3 respectively. Compared with samples gathered from 2008 to 2010, the proportions of subtype A1 and CRF01_AE significantly increased, while the other subtypes significantly decreased in 2015 ( (2)=37.570; =0.013, 99 : 0.010-0.016). Proportions of CRF01_AE and subtype G among males outnumbered the females but the proportions of subtype A1, CRF02_AG and URF among females appeared the other way round ( (2)=15.528; =0.029, 99 : 0.024-0.033). Proportions of CRF02_AG and subtype G among HIV-1 positive Africans were larger than those from other Southeast Asian countries or areas, However, the proportion of CRF01_AE among HIV-1 positive patients from Southeast Asian countries was higher than those patients from other areas ( (2)=39.399; =0.009, 99 : 0.006-0.011). The rates of resistance to any drug of protease inhibitors (PIs), reverse transcriptase inhibitors (RTIs), as well as to PIs, NRTIs, and NNRTIs alone, were 21.9 , 12.3 , 6.1 and 7.0 , respectively. One of nine CRF01_AEs from the HIV-1 positive patients were found closely clustered in those phylogenetic tree (bootstrap=0.855) samples, collected from local patients in Guangzhou. Our findings showed that these foreign subtypes had been spread to the natives, more from the Africans than from the other areas, in Guangzhou. These types of viruses were different from the strains identified locally, suggesting that they might have been brought in by foreigners living with HIV-1, in Guangzhou. Programs related to care, support and behavioral intervention for HIV positive foreigners living in Guangzhou, should be strengthened.
[Mh] Termos MeSH primário: Emigrantes e Imigrantes/estatística & dados numéricos
Genes pol
Infecções por HIV/diagnóstico
Soropositividade para HIV/genética
HIV-1/genética
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Mh] Termos MeSH secundário: China/epidemiologia
Feminino
Genótipo
Infecções por HIV/sangue
Infecções por HIV/etnologia
Infecções por HIV/virologia
Soropositividade para HIV/etnologia
HIV-1/classificação
Seres Humanos
Masculino
Filogenia
Reação em Cadeia da Polimerase
RNA Viral/sangue
Inibidores da Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Reverse Transcriptase Inhibitors); 0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0254-6450.2017.06.023


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[PMID]:28628667
[Au] Autor:Reis MNDG; Bello G; Guimarães ML; Stefani MMA
[Ad] Endereço:Laboratório de Imunologia da AIDS e da Hanseníase, Instituto de Patologia Tropical e Saúde Pública, UFG, Goiânia, Brazil.
[Ti] Título:Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions.
[So] Source:PLoS One;12(6):e0178578, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Brazilian AIDS epidemic has been characterized by an increasing rate of BF1 recombinants and so far eight circulating recombinant forms/CRFs_BF1 have been described countrywide. In this study, pol sequences (protease/PR, reverse transcriptase/RT) of 87 BF1 mosaic isolates identified among 828 patients living in six Brazilian States from three geographic regions (Central West, North, Northeast) were analyzed. Phylogenetic and bootscan analyses were performed to investigate the evolutionary relationship and mosaic structure of BF1 isolates. Those analyses showed that 20.7% of mosaics (18 out of 87) were CRFs-like isolates, mostly represented by CRF28/CRF29_BF-like viruses (14 out of 18). We also identified five highly supported clusters that together comprise 42 out of 87 (48.3%) BF1 sequences, each cluster containing at least five sequences sharing a similar mosaic structure, suggesting possible new unidentified CRFs_BF1. The divergence time of these five potential new CRFs_BF1 clusters was estimated using a Bayesian approach and indicate that they probably originated between the middle 1980s and the middle 1990s. DNA was extracted from whole blood and four overlapping fragments were amplified by PCR providing full/near full length genomes (FLG/NFLG) and partial genomes. Eleven HIV-1 isolates from Cluster # 5 identified in epidemiologically unlinked individuals living in Central West and North regions provided FLG/NFLG/partial genome sequences with identical mosaic structure. These viruses differ from any known CRF_BF1 reported to date and were named CRF90_BF1 by the Los Alamos National Laboratory. This is the 9th CRF_BF1 described in Brazil and the first one identified in Central West and North regions. Our results highlight the importance of continued molecular screening and surveillance studies, especially of full genome sequences to understand the evolutionary dynamics of the HIV-1 epidemic in a country of continental dimensions as Brazil.
[Mh] Termos MeSH primário: Genoma Viral
Infecções por HIV/virologia
HIV-1/genética
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Mh] Termos MeSH secundário: Adulto
Teorema de Bayes
Brasil/epidemiologia
Análise por Conglomerados
Evolução Molecular
Feminino
Genótipo
Infecções por HIV/epidemiologia
HIV-1/isolamento & purificação
Seres Humanos
Masculino
Meia-Idade
Filogenia
RNA Viral/isolamento & purificação
RNA Viral/metabolismo
Recombinação Genética
Análise de Sequência de DNA
Adulto Jovem
Produtos do Gene pol do Vírus da Imunodeficiência Humana/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178578


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[PMID]:28582463
[Au] Autor:Svärd J; Mugusi S; Mloka D; Neogi U; Meini G; Mugusi F; Incardona F; Zazzi M; Sönnerborg A
[Ad] Endereço:Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
[Ti] Título:Drug resistance testing through remote genotyping and predicted treatment options in human immunodeficiency virus type 1 infected Tanzanian subjects failing first or second line antiretroviral therapy.
[So] Source:PLoS One;12(6):e0178942, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Antiretroviral therapy (ART) has been successfully introduced in low-middle income countries. However an increasing rate of ART failure with resistant virus is reported. We therefore described the pattern of drug resistance mutations at antiretroviral treatment (ART) failure in a real-life Tanzanian setting using the remote genotyping procedure and thereafter predicted future treatment options using rule-based algorithm and the EuResist bioinformatics predictive engine. According to national guidelines, the default first-line regimen is tenofovir + lamivudine + efavirenz, but variations including nevirapine, stavudine or emtricitabine can be considered. If failure on first-line ART occurs, a combination of two nucleoside reverse transcriptase inhibitors (NRTIs) and boosted lopinavir or atazanavir is recommended. MATERIALS AND METHODS: Plasma was obtained from subjects with first (n = 174) or second-line (n = 99) treatment failure, as defined by clinical or immunological criteria, as well as from a control group of ART naïve subjects (n = 17) in Dar es Salaam, Tanzania. Amplification of the pol region was performed locally and the amplified DNA fragment was sent to Sweden for sequencing (split genotyping procedure). The therapeutic options after failure were assessed by the genotypic sensitivity score and the EuResist predictive engine. Viral load was quantified in a subset of subjects with second-line failure (n = 52). RESULTS: The HIV-1 pol region was successfully amplified from 55/174 (32%) and 28/99 (28%) subjects with first- or second-line failure, respectively, and 14/17 (82%) ART-naïve individuals. HIV-1 pol sequence was obtained in 82 of these 97 cases (84.5%). Undetectable or very low (<2.6 log10 copies/10-3 L) viral load explained 19 out of 25 (76%) amplification failures in subjects at second-line ART failure. At first and second line failure, extensive accumulation of NRTI (88% and 73%, respectively) and NNRTI (93% and 73%, respectively) DRMs but a limited number of PI DRMs (11% at second line failure) was observed. First line failure subjects displayed a high degree of cross-resistance to second-generation NNRTIs etravirine (ETR; 51% intermediate and 9% resistant) and rilpivirine (RPV; 12% intermediate and 58% resistant), and to abacavir (ABC; 49% resistant) which is reserved for second line therapy in Tanzania. The predicted probability of success with the best salvage regimen at second-line failure decreased from 93.9% to 78.7% when restricting access to the NRTIs, NNRTIs and PIs currently available in Tanzania compared to when including all approved drugs. DISCUSSION: The split genotyping procedure is potential tool to analyse drug resistance in Tanzania but the sensitivity should be evaluated further. The lack of viral load monitoring likely results in a high false positive rate of treatment failures, unnecessary therapy switches and massive accumulation of NRTI and NNRTI mutations. The introduction of regular virological monitoring should be prioritized and integrated with drug resistance studies in resource limited settings.
[Mh] Termos MeSH primário: Fármacos Anti-HIV/uso terapêutico
Farmacorresistência Viral/genética
Genótipo
Infecções por HIV/tratamento farmacológico
HIV-1/genética
[Mh] Termos MeSH secundário: Adulto
Terapia Antirretroviral de Alta Atividade
Sulfato de Atazanavir/uso terapêutico
Benzoxazinas/uso terapêutico
Tomada de Decisão Clínica
Biologia Computacional
Estudos Transversais
Emtricitabina/uso terapêutico
Feminino
Infecções por HIV/virologia
HIV-1/classificação
HIV-1/efeitos dos fármacos
HIV-1/crescimento & desenvolvimento
Seres Humanos
Lamivudina/uso terapêutico
Lopinavir/uso terapêutico
Masculino
Monitorização Fisiológica
Nevirapina/uso terapêutico
Estavudina/uso terapêutico
Tanzânia
Tenofovir/uso terapêutico
Falha de Tratamento
Carga Viral/efeitos dos fármacos
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-HIV Agents); 0 (Benzoxazines); 0 (pol Gene Products, Human Immunodeficiency Virus); 2494G1JF75 (Lopinavir); 2T8Q726O95 (Lamivudine); 4MT4VIE29P (Atazanavir Sulfate); 99DK7FVK1H (Nevirapine); 99YXE507IL (Tenofovir); BO9LE4QFZF (Stavudine); G70B4ETF4S (Emtricitabine); JE6H2O27P8 (efavirenz)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178942


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[PMID]:28551370
[Au] Autor:Li X; Zhu K; Xue Y; Wei F; Gao R; Duerr R; Fang K; Li W; Song Y; Du G; Yan W; Musa TH; Ge Y; Ji Y; Zhong P; Wei P
[Ad] Endereço:Teaching and Research Office of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing, China.
[Ti] Título:Multiple introductions and onward transmission of HIV-1 subtype B strains in Shanghai, China.
[So] Source:J Infect;75(2):160-168, 2017 Aug.
[Is] ISSN:1532-2742
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To investigate the viral genetic evolution, spatial origins and patterns of transmission of HIV-1 subtype B in Shanghai, China. METHODS: A total of 242 Shanghai subtype B and 1519 reference pol sequences were subjected to phylogenetic inference and genetic transmission network analyses. RESULTS: Phylogenetic analysis revealed that subtype B strains circulating in Shanghai were genetically diverse and closely associated with viral sequence lineages in Beijing (76 of 242 [31.4%]), Central China (Henan/Hebei/Hunan/Hubei) (43 of 242 [17.8%]), Chinese Taiwan (20 of 242 [8.3%]), Japan (6 of 242 [2.5%]), and Korea (7 of 242 [2.9%]), suggesting multiple introductions into Shanghai from mainland China and Taiwan, Japan, and Korea. Interestingly, a monophyletic Shanghai lineage (SH-L) (36 of 242 [14.9%]) of HIV-1 subtype B most likely originated from an Argentine strain, transferred through Liaoning infected individuals. In-depth analyses of 195 Shanghai subtype B sequences revealed that a total of 37.9% (n = 74) sequences contributed to 35 transmission networks, whereof 33.8% (n = 25) of the sequences associated with infected individuals from other provinces. CONCLUSIONS: Our new findings reflect the evolution complexity and transmission dynamics of HIV-1 subtype B in Shanghai, which would provide critical information for the design of effective prevention measures against HIV transmission.
[Mh] Termos MeSH primário: Infecções por HIV/transmissão
Infecções por HIV/virologia
HIV-1/genética
[Mh] Termos MeSH secundário: Extremo Oriente/epidemiologia
Feminino
Infecções por HIV/epidemiologia
HIV-1/classificação
Seres Humanos
Masculino
Filogenia
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170529
[St] Status:MEDLINE


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[PMID]:28425872
[Au] Autor:Lima K; Leal É; Cavalcanti AMS; Salustiano DM; de Medeiros LB; da Silva SP; Lacerda HR
[Ad] Endereço:1​Tropical Medicine, Federal University of Pernambuco, Recife, PE, Brazil.
[Ti] Título:Increase in human immunodeficiency virus 1 diversity and detection of various subtypes and recombinants in north-eastern Brazil.
[So] Source:J Med Microbiol;66(4):526-535, 2017 Apr.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Diverse human immunodeficiency virus 1 (HIV-1) subtypes and circulating recombinant forms are found in Brazil. The majority of HIV-1 molecular epidemiological studies in Brazil have been conducted in the southern and south-eastern regions of the country, although several recent studies in the north-eastern region have addressed this issue. The objective of this study was to molecularly characterize HIV-1 circulating in Pernambuco, north-eastern Brazil. METHODOLOGY: A total of 64 samples were collected from 2002 to 2003, and another 103 were collected from 2007 to 2009. The protease and partial reverse transcriptase regions of the HIV-1 polymerase-encoding (pol) gene were sequenced, and subtyping, recombination and phylogenetic analyses were performed.Results/Key findings. Subtype B (60.9 %) was found to be predominant, followed by HIV-1 F (31.4 %). Several BF recombinants (4.2 %), and BC and AG recombinants were also identified. The intra-subtype genetic diversity was estimated to be 0.065 (sd±0.004) for HIV-1 B and 0.055 (sd±0.004) for HIV-1 F, reflecting a greater accumulation of mutations in subtype B (P<0.01). More codons were found to be under positive selective pressure in samples collected from 2007 to 2009, from individuals with a T-cell count≥200 cells mm-3 and from women. Coalescence data indicated that the subtype F population has been continuously expanding. CONCLUSIONS: HIV-1 shows high genetic diversity in the state of Pernambuco. Thus, additional molecular evaluations of circulating strains will provide a better understanding of the epidemic and may lead to more effective preventive strategies.
[Mh] Termos MeSH primário: Infecções por HIV/epidemiologia
Protease de HIV/genética
HIV-1/genética
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Mh] Termos MeSH secundário: Adulto
Sequência de Bases
Brasil/epidemiologia
Feminino
Variação Genética/genética
Infecções por HIV/virologia
Seres Humanos
Masculino
Epidemiologia Molecular
Filogenia
RNA Viral/genética
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (pol Gene Products, Human Immunodeficiency Virus); EC 3.4.23.- (HIV Protease)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000447


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Tanuri, Amilcar
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[PMID]:28333295
[Au] Autor:Andrade SD; Sabidó M; Monteiro WM; Benzaken AS; Tanuri A
[Ad] Endereço:Tropical Medicine Foundation Doctor Heitor Vieira Dourado (FMT-HVD), Av. Pedro Teixeira 25, Manaus, Amazonas, CEP: 69040-000, Brazil.
[Ti] Título:Drug resistance in antiretroviral-naive children newly diagnosed with HIV-1 in Manaus, Amazonas.
[So] Source:J Antimicrob Chemother;72(6):1774-1783, 2017 Jun 01.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: To determine the prevalence of drug resistance mutations (DRM), the prevalence of drug susceptibility [transmitted drug resistance (TDR)] and the prevalence of HIV-1 variants among treatment-naive HIV-infected children in Manaus, Amazonas state, Brazil. Methods: Children born to HIV-infected mothers and diagnosed with HIV in an HIV reference service centre and with available pol sequence between 2010 and 2015 prior to antiretroviral initiation were included. TDR was identified using the Calibrated Population Resistance Tool. HIV-1 subtypes were defined by Rega and phylogenetic analyses. Results: One hundred and seventeen HIV-infected children with a median age of 3.7 years were included. Among them, 28.2% had been exposed to some form of prevention of mother-to-child transmission (PMTCT). HIV DRM were present in 21.4% of all children. Among PMTCT-exposed children, 3% had NRTI mutations, 15.2% had NNRTI mutations and 3% had PI mutations. Among PMTCT-unexposed children, 1.2% had NRTI mutations, 21.4% had non-NNRTI mutations and 1.2% had PI mutations. The most common DRM was E138A (8.5%). The prevalence of TDR was 16.2%; 21.1% among PMTCT-exposed children and 14.3% among PMTC-unexposed children. The analysis of HIV-1 subtypes revealed that 80.2% were subtype B, 6.0% were subtype C, 3.4% were subtype F1 and 10.3% were possible unique recombinant forms (BF1, 4.3%; DB, 4.3%; BC, 0.9%; KC, 0.9%). Conclusions: We report a high prevalence of DRM in this population, including in almost a quarter of children with no reported PMTCT. The high prevalence of TDR observed might compromise ART effectiveness. Results show extensive HIV-1 diversity and expansion of subtype C, which highlights the need for surveillance of HIV-1 subtypes in Amazonas state.
[Mh] Termos MeSH primário: Fármacos Anti-HIV/farmacologia
Fármacos Anti-HIV/uso terapêutico
Farmacorresistência Viral Múltipla/genética
Infecções por HIV/tratamento farmacológico
Infecções por HIV/virologia
HIV-1/efeitos dos fármacos
HIV-1/genética
[Mh] Termos MeSH secundário: Terapia Antirretroviral de Alta Atividade
Brasil
Criança
Pré-Escolar
Estudos de Coortes
Feminino
Genótipo
Infecções por HIV/epidemiologia
Infecções por HIV/transmissão
HIV-1/isolamento & purificação
Seres Humanos
Lactente
Transmissão Vertical de Doença Infecciosa
Masculino
Mães
Mutação
Filogenia
RNA Viral/sangue
RNA Viral/genética
Estudos Retrospectivos
Análise de Sequência de DNA
Carga Viral
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-HIV Agents); 0 (RNA, Viral); 0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkx025


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[PMID]:28329933
[Au] Autor:Deng YQ; Li JJ; Fang NY; Wang B; Wang JW; Liang SS; Shen ZY; Lan GH; Zhang HM; Wu XH; Lu HX; Ge XM
[Ad] Endereço:Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning 530023, China.
[Ti] Título:[Study on HIV-1 subtype among elderly male clients and female sex workers of low-cost venues in Guangxi Zhuang Autonomous Region, China].
[So] Source:Zhonghua Liu Xing Bing Xue Za Zhi;38(3):326-330, 2017 Mar 10.
[Is] ISSN:0254-6450
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To understand HIV-1 subtype characteristics and transmission clusters in elderly male clients and female sex workers (FSWs) of low-cost commercial sex venues in Guangxi Zhuang Autonomous Region, China. A cross sectional survey was conducted in FSWs and elderly male clients (≥50 years) of low-cost commercial sex venues in 4 cities and 9 counties in Guangxi Zhuang Autonomous Region by convenient sampling in 2012. The blood sample was collected from each case for HIV-1 antibody detection. The gene fragments were amplified and sequenced from viral RNA template extracted from plasma samples. The phylogenetic tree was constructed and the subtypes were identified. A total of 4 048 elderly male clients and 784 FSWs were surveyed, and 116 HIV-1 infections were detected, the positive rate was 2.5% (103/4 048) in the clients and 1.7% (13/784) in FSWs. The gene amplification and sequencing of HIV-1 detected in 84 blood samples indicated that 53 gene sequences were successfully determined (48 blood samples from elderly male clients and 5 blood samples from FSWs). Among 53 sequences, 48(90.6% ), 4(7.5% ), and 1(1.9% ) sequences were identified as CRF01_AE, CRF08_BC, and CRF07_BC, respectively. Two transmission clusters were identified among CRF01_AE, including 4 sub-clusters. One transmission cluster was identified among CRF08_BC. The transmission cluster or sub-cluster were from the infected individuals at same low-cost commercial sex venue, or different low-cost commercial sex venues in the same town, or same place, or adjacent villages and towns. CRF01_AE was the predominant HIV-1 subtype among elderly male clients and FSWs of low-cost commercial sex venues in Guangxi Zhuang Autonomous Region, circulating in same venue or adjacent villages and towns. The HIV-1 positive male clients and FSWs might play an important role in the spread of the strains.
[Mh] Termos MeSH primário: Infecções por HIV/epidemiologia
HIV-1/genética
Profissionais do Sexo
Produtos do Gene pol do Vírus da Imunodeficiência Humana/genética
[Mh] Termos MeSH secundário: Fatores Etários
Idoso
Sequência de Bases
China/epidemiologia
Estudos Transversais
Feminino
Genes pol
Genótipo
Anticorpos Anti-HIV
Infecções por HIV/transmissão
Infecções por HIV/virologia
Seres Humanos
Masculino
Filogenia
Reação em Cadeia da Polimerase
RNA Viral/sangue
Distribuição Espacial da População
Trabalho Sexual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HIV Antibodies); 0 (RNA, Viral); 0 (pol Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0254-6450.2017.03.010



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