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[PMID]:28946120
[Au] Autor:Zhao F; Jiang G; Wei P; Wang H; Ru S
[Ad] Endereço:Marine Life Science College, Ocean University of China, 5 Yushan Road, Qingdao, 266003 Shandong Province, PR China.
[Ti] Título:Bisphenol S exposure impairs glucose homeostasis in male zebrafish (Danio rerio).
[So] Source:Ecotoxicol Environ Saf;147:794-802, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bisphenol S (BPS) is a substitute of the plastic additive bisphenol A (BPA). Its concentrations detected in surface waters and urine samples are on the same order of magnitude as BPA. Human exposure to BPA has been implicated in the development of diabetes mellitus; however, whether BPS can disrupt glucose homeostasis and increase blood glucose concentration remains unclear. We extensively investigated the effects of environmentally relevant concentrations of BPS on glucose metabolism in male zebrafish (Danio rerio) and the underlying mechanisms of these effects. Male zebrafish were exposed to 1, 10, or 100µg/L of BPS for 28 d. Fasting blood glucose (FBG) levels, glycogen levels in the liver and muscle, and mRNA levels of key glucose metabolic enzymes and the activities of the encoded proteins in tissues were evaluated to assess the effect of BPS on glucose metabolism. Plasma insulin levels and expression of preproinsulin and glucagon genes in the visceral tissue were also evaluated. Compared with the control group, exposure to 1 and 10µg/L of BPS significantly increased FBG levels but decreased insulin levels. Gluconeogenesis and glycogenolysis in the liver were promoted, and glycogen synthesis in the liver and muscle and glycolysis in the muscle were inhibited. Exposure to 100µg/L of BPS did not significantly alter plasma insulin and blood glucose levels, but nonetheless pronouncedly interfered with gluconeogenesis, glycogenolysis, glycolysis, and glycogen synthesis. Our data indicates that BPS at environmentally relevant concentrations impairs glucose homeostasis of male zebrafish possibly by hampering the physiological effect of insulin; higher BPS doses also pronouncedly interfered with glucose metabolism.
[Mh] Termos MeSH primário: Glucose/metabolismo
Homeostase/efeitos dos fármacos
Fenóis/toxicidade
Sulfonas/toxicidade
Poluentes Químicos da Água/toxicidade
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Glucagon/genética
Glicogênio/biossíntese
Insulina/sangue
Insulina/genética
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Músculos/efeitos dos fármacos
Músculos/metabolismo
Precursores de Proteínas/sangue
Precursores de Proteínas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Phenols); 0 (Protein Precursors); 0 (Sulfones); 0 (Water Pollutants, Chemical); 61116-24-3 (preproinsulin); 80-09-1 (bis(4-hydroxyphenyl)sulfone); 9005-79-2 (Glycogen); 9007-92-5 (Glucagon); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


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[PMID]:29390406
[Au] Autor:Zhang CL; Xie S; Qiao X; An YM; Zhang Y; Li L; Guo XB; Zhang FC; Wu LL
[Ad] Endereço:Department of Physiology and Pathophysiology, Key Laboratory of Molecular Cardiovascular Science, Beijing Key Laboratory of Cardiovascular Receptors Research, Ministry of Education.
[Ti] Título:Plasma endothelin-1-related peptides as the prognostic biomarkers for heart failure: A PRISMA-compliant meta-analysis.
[So] Source:Medicine (Baltimore);96(50):e9342, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Most studies reported that high plasma endothelin-1 (ET-1), big ET-1, and C-terminal proET-1 (CT-proET-1) were correlated with poor prognosis of heart failure (HF). However, available evidence remains controversial. To help solve the debate, we collected all the available studies and performed a meta-analysis. METHODS: We searched the databases covering Embase, PubMed, Ovid, and Web of Science on June 28, 2017. The hazard ratio (HR) or risk ratio (RR) and its 95% confidence intervals (CIs) were collected and calculated by use of a random-effect model. Heterogeneity was assessed by Cochran's Q test, and publication bias was assessed by funnel plots with Egger's and Begg's linear regression test. RESULTS: Thirty-two studies with 18,497 patients were included in the analysis. Results showed that circulating ET-1, big ET-1, and CT-proET-1 were positively correlated with high risk of adverse outcomes, with pooled RRs (95% CIs) of 2.22 (1.82-2.71, P < .001), 2.47 (1.93-3.17, P < .001), and 2.27 (1.57-3.29, P < .001), respectively. In the subgroup of death as primary outcome, the pooled RRs (95% CIs) were 2.13 (1.68-2.70, P < .001), 2.55 (1.82-3.57, P < .001), and 2.02 (1.39-2.92, P < .001) for ET-1, big ET-1, and CT-proET-1, respectively. No significant publication bias was observed in this study. CONCLUSION: Our meta-analysis provided evidence that increased plasma levels of ET-1, big ET-1, and CT-proET-1 were associated with poor prognosis or mortality for HF populations.
[Mh] Termos MeSH primário: Biomarcadores/sangue
Endotelina-1/sangue
Insuficiência Cardíaca/sangue
[Mh] Termos MeSH secundário: Seres Humanos
Fragmentos de Peptídeos/sangue
Prognóstico
Precursores de Proteínas/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS
[Nm] Nome de substância:
0 (Biomarkers); 0 (Endothelin-1); 0 (Peptide Fragments); 0 (Protein Precursors); 0 (proendothelin 1)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009342


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[PMID]:29352321
[Au] Autor:Jacobi CLJ; Stein C
[Ad] Endereço:Klinik für Anästhesiologie und operative Intensivmedizin, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Berlin, Germany.
[Ti] Título:Inflammatory-linked changes in CpG island methylation of three opioid peptide genes in a rat model for pain.
[So] Source:PLoS One;13(1):e0191698, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Expression of the opioid peptide genes proopiomelanocortin (Pomc), proenkephalin (Penk), and prodynorphin (Pdyn), in immune cells plays a key role in endogenous pain control. In a rat model of painful unilateral paw inflammation, we isolated cells from popliteal lymph nodes and evaluated the role of CpG island C5-methylation on the transcriptional activation of those genes. Using methylated DNA immunoprecipitation, we sorted gDNA into methylated (me) and non-me fractions and then determined the CpG island methylation status of each fraction via quantitative Real Time-PCR (qRT-PCR). In silico analysis by MethPrimer software identified one CpG island in Pdyn and three each in Pomc and Penk. No substantial changes in C5-methylation of any gene were observed. In conclusion, the CpG island methylation status does not seem to be a key regulator of opioid gene activation in immune cells during peripheral tissue inflammation.
[Mh] Termos MeSH primário: Ilhas de CpG
Metilação de DNA
Encefalinas/genética
Inflamação/genética
Dor/genética
Pró-Opiomelanocortina/genética
Precursores de Proteínas/genética
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Regulação da Expressão Gênica
Inflamação/metabolismo
Masculino
Dor/metabolismo
Ratos
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enkephalins); 0 (Protein Precursors); 0 (proenkephalin); 66796-54-1 (Pro-Opiomelanocortin); 93443-35-7 (preproenkephalin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191698


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[PMID]:29051075
[Au] Autor:Zhang H; Qin G; Sun J; Zhang B; Lin Q
[Ad] Endereço:CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China.
[Ti] Título:The evolution and functional characterization of lined seahorse (Hippocampus erectus) CCKs involved in fasting and thermal stress response.
[So] Source:Gen Comp Endocrinol;255:56-63, 2018 Jan 01.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The peptide cholecystokinin (CCK) plays an important role in the regulation of vertebrate appetite and feeding behaviour. In the present study, the full-length cDNA and genomic DNA sequences of two CCK precursors were cloned and analysed in the Syngnathidae fish, the lined seahorse (Hippocampus erectus). Both CCK1 and CCK2 in the seahorse consist of four exons. The sequence of the octapeptide of seahorse CCK1 (DYMGWMDF) was the same as that of the chicken and human, while the octapeptide of seahorse CCK2 (DYEGWMDF) was unique among vertebrates. According to the phylogenetic analysis, two types of CCKs were produced by teleost-specific genome duplication (TGD). Both CCK1 and CCK2 were highly expressed in the brain, while detectable amounts of CCK1 mRNA in the brood pouch and CCK2 mRNA in the intestine were also found. Both CCK1 and CCK2 mRNA levels significantly increased during the transition from endogenous to exogenous nutrition. Additionally, fasting induced a significant increase in the CCK1 mRNA expression in the brain of juvenile seahorses but had no effect on CCK2 transcript levels. In addition, the CCK1 and CCK2 mRNA levels in the seahorse brain significantly increased after a high-temperature treatment. Thus, the mRNA expression of CCK had obvious tissue specificities and this preliminary study opens new avenues for further functional studies on the endocrine regulations of CCK in the transition from endogenous to exogenous nutrition, food intake regulation and metabolism in the seahorse.
[Mh] Termos MeSH primário: Colecistocinina/genética
Evolução Molecular
Jejum/fisiologia
Smegmamorpha/metabolismo
Estresse Fisiológico
Temperatura Ambiente
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Fenômenos Fisiológicos da Nutrição Animal
Animais
Sequência de Bases
Colecistocinina/química
Colecistocinina/metabolismo
Clonagem Molecular
DNA Complementar/genética
Seres Humanos
Larva/metabolismo
Especificidade de Órgãos
Filogenia
Precursores de Proteínas/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Protein Precursors); 0 (RNA, Messenger); 78206-77-6 (procholecystokinin); 9011-97-6 (Cholecystokinin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


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[PMID]:29281094
[Au] Autor:Maeda Y; Kudo S; Tsushima K; Sato E; Kubota C; Kayamori A; Bochimoto H; Koga D; Torii S; Gomi H; Watanabe T; Hosaka M
[Ad] Endereço:Department of Biotechnology, Laboratory of Molecular Life Sciences, Akita Prefectural University, Akita, Japan.
[Ti] Título:Impaired Processing of Prohormones in Secretogranin III-Null Mice Causes Maladaptation to an Inadequate Diet and Stress.
[So] Source:Endocrinology;159(2):1213-1227, 2018 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Secretogranin III (SgIII), a member of the granin family, binds both to another granin, chromogranin A (CgA), and to a cholesterol-rich membrane that is destined for secretory granules (SGs). The knockdown of SgIII in adrenocorticotropic hormone (ACTH)-producing AtT-20 cells largely impairs the regulated secretion of CgA and ACTH. To clarify the physiological roles of SgIII in vivo, we analyzed hormone secretion and SG biogenesis in newly established SgIII-knockout (KO) mice. Although the SgIII-KO mice were viable and fertile and exhibited no overt abnormalities under ordinary rearing conditions, a high-fat/high-sucrose diet caused pronounced obesity in the mice. Furthermore, in the SgIII-KO mice compared with wild-type (WT) mice, the stimulated secretion of active insulin decreased substantially, whereas the storage of proinsulin increased in the islets. The plasma ACTH was also less elevated in the SgIII-KO mice than in the WT mice after chronic restraint stress, whereas the storage level of the precursor proopiomelanocortin in the pituitary gland was somewhat increased. These findings suggest that the lack of SgIII causes maladaptation of endocrine cells to an inadequate diet and stress by impairing the proteolytic conversion of prohormones in SGs, whereas SG biogenesis and the basal secretion of peptide hormones under ordinary conditions are ensured by the compensatory upregulation of other residual granins or factors.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Cromograninas/genética
Cromograninas/metabolismo
Dieta/efeitos adversos
Precursores de Proteínas/genética
Precursores de Proteínas/metabolismo
Estresse Fisiológico/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Masculino
Doenças Metabólicas/genética
Doenças Metabólicas/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Processamento de Proteína Pós-Traducional
Estresse Fisiológico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromogranins); 0 (Protein Precursors); 0 (secretogranin III)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00636


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[PMID]:28466092
[Au] Autor:Montagud-Romero S; Nuñez C; Blanco-Gandia MC; Martínez-Laorden E; Aguilar MA; Navarro-Zaragoza J; Almela P; Milanés MV; Laorden ML; Miñarro J; Rodríguez-Arias M
[Ad] Endereço:Department of Psychobiology, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, 21, 46010, Valencia, Spain.
[Ti] Título:Repeated social defeat and the rewarding effects of cocaine in adult and adolescent mice: dopamine transcription factors, proBDNF signaling pathways, and the TrkB receptor in the mesolimbic system.
[So] Source:Psychopharmacology (Berl);234(13):2063-2075, 2017 Jul.
[Is] ISSN:1432-2072
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Repeated social defeat (RSD) increases the rewarding effects of cocaine in adolescent and adult rodents. OBJECTIVE: The aim of the present study was to compare the long-term effects of RSD on the conditioned rewarding effects of cocaine and levels of the transcription factors Pitx3 and Nurr1 in the ventral tegmental area (VTA), the dopamine transporter (DAT), the D2 dopamine receptor (D2DR) and precursor of brain-derived neurotrophic factor (proBDNF) signaling pathways, and the tropomyosin-related kinase B (TrkB) receptor in the nucleus accumbens (NAc) in adult and adolescent mice. METHODS: Male adolescent and young adult OF1 mice were exposed to four episodes of social defeat and were conditioned 3 weeks later with 1 mg/kg of cocaine. In a second set of mice, the expressions of the abovementioned dopaminergic and proBDNF and TrkB receptor were measured in VTA and NAc, respectively. RESULTS: Adolescent mice experienced social defeats less intensely than their adult counterparts and produced lower levels of corticosterone. However, both adult and adolescent defeated mice developed conditioned place preference for the compartment associated with this low dose of cocaine. Furthermore, only adolescent defeated mice displayed diminished levels of the transcription factors Pitx3 in the VTA, without changes in the expression of DAT and D2DR in the NAc. In addition, stressed adult mice showed a decreased expression of proBDNF and the TrkB receptor, while stressed adolescent mice exhibited increased expression of latter without changes in the former. CONCLUSION: Our findings suggest that dopaminergic pathways and proBDNF signaling and TrkB receptors play different roles in social defeat-stressed mice exposed to cocaine.
[Mh] Termos MeSH primário: Fator Neurotrófico Derivado do Encéfalo/fisiologia
Encéfalo/metabolismo
Cocaína/farmacologia
Condicionamento Clássico/efeitos dos fármacos
Corticosterona/metabolismo
Glicoproteínas de Membrana/metabolismo
Núcleo Accumbens/efeitos dos fármacos
Precursores de Proteínas/fisiologia
Receptor trkB/metabolismo
Receptores de Dopamina D2/metabolismo
Fatores de Transcrição/metabolismo
Área Tegmentar Ventral/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Encéfalo/fisiologia
Fator Neurotrófico Derivado do Encéfalo/química
Fator Neurotrófico Derivado do Encéfalo/metabolismo
Condicionamento Clássico/fisiologia
Corticosterona/química
Dopamina/metabolismo
Masculino
Glicoproteínas de Membrana/química
Camundongos
Precursores de Proteínas/química
Receptor trkB/química
Receptores de Dopamina D2/química
Recompensa
Estresse Psicológico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brain-Derived Neurotrophic Factor); 0 (DRD2 protein, human); 0 (Membrane Glycoproteins); 0 (Protein Precursors); 0 (Receptors, Dopamine D2); 0 (Transcription Factors); 0 (brain-derived neurotrophic factor precursor); EC 2.7.10.1 (Receptor, trkB); EC 2.7.10.1 (tropomyosin-related kinase-B, human); I5Y540LHVR (Cocaine); VTD58H1Z2X (Dopamine); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s00213-017-4612-y


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[PMID]:29261728
[Au] Autor:Choudhary D; Kumar A; Magliery TJ; Sotomayor M
[Ad] Endereço:Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, United States of America.
[Ti] Título:Using thermal scanning assays to test protein-protein interactions of inner-ear cadherins.
[So] Source:PLoS One;12(12):e0189546, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein-protein interactions play a crucial role in biological processes such as cell-cell adhesion, immune system-pathogen interactions, and sensory perception. Understanding the structural determinants of protein-protein complex formation and obtaining quantitative estimates of their dissociation constant (KD) are essential for the study of these interactions and for the discovery of new therapeutics. At the same time, it is equally important to characterize protein-protein interactions in a high-throughput fashion. Here, we use a modified thermal scanning assay to test interactions of wild type (WT) and mutant variants of N-terminal fragments (EC1+2) of cadherin-23 and protocadherin-15, two proteins essential for inner-ear mechanotransduction. An environmentally sensitive fluorescent dye (SYPRO orange) is used to monitor melting temperature (Tm) shifts of protocadherin-15 EC1+2 (pcdh15) in the presence of increasing concentrations of cadherin-23 EC1+2 (cdh23). These Tm shifts are absent when we use proteins containing deafness-related missense mutations known to disrupt cdh23 binding to pcdh15, and are increased for some rationally designed mutants expected to enhance binding. In addition, surface plasmon resonance binding experiments were used to test if the Tm shifts correlated with changes in binding affinity. We used this approach to find a double mutation (cdh23(T15E)- pcdh15(G16D)) that enhances binding affinity of the cadherin complex by 1.98 kJ/mol, roughly two-fold that of the WT complex. We suggest that the thermal scanning methodology can be used in high-throughput format to quickly compare binding affinities (KD from nM up to 100 µM) for some heterodimeric protein complexes and to screen small molecule libraries to find protein-protein interaction inhibitors and enhancers.
[Mh] Termos MeSH primário: Caderinas/metabolismo
Mapeamento de Interação de Proteínas/métodos
Precursores de Proteínas/metabolismo
[Mh] Termos MeSH secundário: Animais
Caderinas/química
Orelha Interna/metabolismo
Cinética
Camundongos
Modelos Moleculares
Proteínas Mutantes/metabolismo
Mutação/genética
Ligação Proteica
Desnaturação Proteica
Precursores de Proteínas/química
Soluções
Ressonância de Plasmônio de Superfície
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadherins); 0 (Cdh23 protein, mouse); 0 (Mutant Proteins); 0 (Pcdh15 protein, mouse); 0 (Protein Precursors); 0 (Solutions)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189546


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[PMID]:28455445
[Au] Autor:Chen R; Jin G; McIntyre TM
[Ad] Endereço:From the Departments of Cellular and Molecular Medicine and.
[Ti] Título:The soluble protease ADAMDEC1 released from activated platelets hydrolyzes platelet membrane pro-epidermal growth factor (EGF) to active high-molecular-weight EGF.
[So] Source:J Biol Chem;292(24):10112-10122, 2017 06 16.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Platelets are the sole source of EGF in circulation, yet how EGF is stored or released from stimulated cells is undefined. In fact, we found platelets did not store EGF, synthesized as a single 6-kDa domain in pro-EGF, but rather expressed intact pro-EGF precursor on granular and plasma membranes. Activated platelets released high-molecular-weight (HMW)-EGF, produced by a single cleavage between the EGF and the transmembrane domains of pro-EGF. We synthesized a fluorogenic peptide encompassing residues surrounding the putative sessile arginyl residue and found stimulated platelets released soluble activity that cleaved this pro-EGF peptide. High throughput screening identified chymostatins, bacterial peptides with a central cyclic arginyl structure, as inhibitors of this activity. In contrast, the matrix metalloproteinase/TACE (tumor necrosis factor-α-converting enzyme) inhibitor GM6001 was ineffective. Stimulated platelets released the soluble protease ADAMDEC1, recombinant ADAMDEC1 hydrolyzed pro-EGF , and this activity was inhibited by chymostatin and not GM6001. Biotinylating platelet surface proteins showed ADAMDEC1 hydrolyzed surface pro-EGF to HMW-EGF that stimulated HeLa EGF receptor (EGFR) reporter cells and EGFR-dependent tumor cell migration. This proteolysis was inhibited by chymostatin and not GM6001. Metabolizing pro-EGF Arg to citrulline with recombinant polypeptide arginine deiminase 4 (PAD4) abolished ADAMDEC1-catalyzed pro-EGF peptidolysis, while pretreating intact platelets with PAD4 suppressed ADAMDEC1-, thrombin-, or collagen-induced release of HMW-EGF. We conclude that activated platelets release ADAMDEC1, which hydrolyzes pro-EGF to soluble HMW-EGF, that HMW-EGF is active, that proteolytic cleavage of pro-EGF first occurs at the C-terminal arginyl residue of the EGF domain, and that proteolysis is the regulated and rate-limiting step in generating soluble EGF bioactivity from activated platelets.
[Mh] Termos MeSH primário: Proteínas ADAM/metabolismo
Plaquetas/enzimologia
Membrana Celular/enzimologia
Fator de Crescimento Epidérmico/metabolismo
Ativação Plaquetária
Precursores de Proteínas/metabolismo
Receptor do Fator de Crescimento Epidérmico/agonistas
[Mh] Termos MeSH secundário: Proteínas ADAM/antagonistas & inibidores
Proteínas ADAM/química
Proteínas ADAM/genética
Animais
Plaquetas/metabolismo
Células CHO
Linhagem Celular Tumoral
Membrana Celular/metabolismo
Cricetulus
Fator de Crescimento Epidérmico/química
Fator de Crescimento Epidérmico/genética
Seres Humanos
Hidrolases/genética
Hidrolases/metabolismo
Cinética
Peso Molecular
Oligopeptídeos/farmacologia
Inibidores de Proteases/farmacologia
Domínios e Motivos de Interação entre Proteínas
Precursores de Proteínas/química
Precursores de Proteínas/genética
Processamento de Proteína Pós-Traducional/efeitos dos fármacos
Desiminases de Arginina em Proteínas
Proteólise/efeitos dos fármacos
Receptor do Fator de Crescimento Epidérmico/genética
Receptor do Fator de Crescimento Epidérmico/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Oligopeptides); 0 (Protease Inhibitors); 0 (Protein Precursors); 0 (Recombinant Proteins); 62229-50-9 (Epidermal Growth Factor); 9076-44-2 (chymostatin); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 3.- (Hydrolases); EC 3.4.24.- (ADAM Proteins); EC 3.4.24.- (decysin); EC 3.5.3.15 (Protein-Arginine Deiminases); EC 3.5.3.15 (peptidylarginine deiminase type IV)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.771642


  9 / 28231 MEDLINE  
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Fotocópia
[PMID]:29183533
[Au] Autor:Iida H; Aihara T; Ikuta S; Yamanaka N
[Ti] Título:Predictive Factors for Treatment Failure after Peritoneovenous Shunt for Hepatic Ascites.
[So] Source:Am Surg;83(11):1289-1293, 2017 Nov 01.
[Is] ISSN:1555-9823
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peritoneovenous shunt (PVS) is used to treat refractory ascites. Here, we identify predictive factors for inhospital death after PVS placement. Thirty-five patients with refractory ascites related to liver cirrhosis and/or hepatocellular carcinoma (HCC) who underwent PVS placement between February 2005 and February 2013 were included in the study. Group A comprised 13 patients for whom the PVS placement outcome was inhospital death. Group B comprised 22 patients who were discharged after PVS placement without complications. Patient background and laboratory data were analyzed to identify risk factors for inhospital death. HCC prevalence in Groups A and B was 92 and 55 per cent, respectively (P = 0.02) and that of portal venous tumor thrombus (PVTT) was 54 and 9 per cent, respectively (P = 0.003). The mean des-γ-carboxy prothrombin (DCP) level in both groups was 15,553 ± 49,330 and 787 ± 2600 mAU/mL, respectively (P = 0.009). Multivariate analysis revealed that the presence of PVTT was the only independent predictor of inhospital death (P = 0.007). The presence of PVTT, HCC, and elevated des-γ-carboxy prothrombin levels are predictors of inhospital death after PVS placement. Therefore, PVS should not be used to treat refractory ascites in patients with these predictors, particularly with PVTT.
[Mh] Termos MeSH primário: Ascite/cirurgia
Carcinoma Hepatocelular/cirurgia
Cirrose Hepática/cirurgia
Neoplasias Hepáticas/cirurgia
Derivação Peritoneovenosa/mortalidade
[Mh] Termos MeSH secundário: Idoso
Ascite/mortalidade
Biomarcadores/metabolismo
Carcinoma Hepatocelular/mortalidade
Feminino
Mortalidade Hospitalar
Seres Humanos
Cirrose Hepática/mortalidade
Neoplasias Hepáticas/mortalidade
Masculino
Células Neoplásicas Circulantes
Precursores de Proteínas/metabolismo
Protrombina/metabolismo
Estudos Retrospectivos
Fatores de Risco
Falha de Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Protein Precursors); 53230-14-1 (acarboxyprothrombin); 9001-26-7 (Prothrombin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


  10 / 28231 MEDLINE  
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Texto completo
[PMID]:29079276
[Au] Autor:Pankhurst MW; Shorakae S; Rodgers RJ; Teede HJ; Moran LJ
[Ad] Endereço:Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand. Electronic address: michael.pankhurst@otago.ac.nz.
[Ti] Título:Efficacy of predictive models for polycystic ovary syndrome using serum levels of two antimüllerian hormone isoforms (proAMH and AMH ).
[So] Source:Fertil Steril;108(5):851-857.e2, 2017 Nov.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To compare total antimüllerian hormone (AMH), proAMH, AMH , and the ratio of the two forms in predictive models for polycystic ovary syndrome (PCOS) diagnosis. Total AMH consists of proAMH (inactive precursor) and AMH (receptor-competent), but neither isoform has been tested individually for their ability to predict PCOS diagnosis. DESIGN: Cross-sectional study using biobanked samples collected between July 2008 and January 2010. SETTING: Not applicable. PATIENT(S): Overweight, premenopausal women aged 18-45 years with PCOS (n = 45, with 21 fulfilling National Institutes of Health diagnostic criteria and 24 fulfilling European Society for Human Reproduction and Embryology/American Society for Reproductive Medicine (ESHRE) criteria, but not National Institutes of Health criteria) and without PCOS (n = 23 controls). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Serum concentrations of proAMH and total AMH (proAMH and AMH combined) were determined by immunoassay. The AMH concentrations were calculated by subtraction ([AMH ] = [total AMH] - [proAMH]). Relative levels of proAMH were expressed as the AMH prohormone index (API = [ProAMH]/[Total AMH] × 100). RESULT(S): In women with PCOS, total AMH, proAMH, and AMH levels were higher, and the API was lower (P=.010), than in controls indicating increased conversion of proAMH to AMH . Receiver-operating characteristic analysis for proAMH (area under the curve [AUC] = 0.82), AMH (AUC = 0.86), and API (AUC = 0.70) did not improve the prediction for PCOS when compared with total AMH (AUC = 0.86). CONCLUSION(S): The proAMH and AMH do not appear to improve the ability to predict a diagnosis of PCOS beyond total AMH assays. However, the ratio of inactive proAMH precursor to receptor-competent AMH (API) differs in women with PCOS relative to unaffected controls indicating that AMH signaling mechanisms may be altered in women with PCOS.
[Mh] Termos MeSH primário: Hormônio Antimülleriano/sangue
Síndrome do Ovário Policístico/sangue
Síndrome do Ovário Policístico/diagnóstico
Precursores de Proteínas/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Área Sob a Curva
Biomarcadores/sangue
Estudos de Casos e Controles
Estudos Transversais
Feminino
Seres Humanos
Meia-Idade
Valor Preditivo dos Testes
Prognóstico
Isoformas de Proteínas
Curva ROC
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Protein Isoforms); 0 (Protein Precursors); 80497-65-0 (Anti-Mullerian Hormone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171029
[St] Status:MEDLINE



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