[PMID]: | 29309783 |
[Au] Autor: | Picchio MS; Sánchez VR; Arcon N; Soto AS; Perrone Sibilia M; Aldirico MLA; Urrutia M; Moretta R; Fenoy IM; Goldman A; Martin V |
[Ad] Endereço: | Laboratorio de Inmunología, Vacunas y Alergia, CESyMA, Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Campus Migueletes, Martín de Irigoyen 3100 C.P., 1650 San Martín, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 |
[Ti] Título: | Vaccine potential of antigen cocktails composed of recombinant Toxoplasma gondii TgPI-1, ROP2 and GRA4 proteins against chronic toxoplasmosis in C3H mice. |
[So] Source: | Exp Parasitol;185:62-70, 2018 Feb. |
[Is] ISSN: | 1090-2449 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | The development of an effective and safe vaccine to prevent Toxoplasma gondii infection is an important aim due to the great clinical and economic impact of this parasitosis. We have previously demonstrated that immunization with the serine protease inhibitor-1 (TgPI-1) confers partial protection to C3H/HeN and C57BL/6 mice. In order to improve the level of protection, in this work, we combined this novel antigen with ROP2 and/or GRA4 recombinant proteins (rTgPI-1+rROP2, rTgPI-1+rGRA4, rTgPI-1+rROP2+rGRA4) to explore the best combination against chronic toxoplasmosis in C3H/HeN mice. All tested vaccine formulations, administered following a homologous prime-boost protocol that combines intradermal and intranasal routes, conferred partial protection as measured by the reduction of brain cyst burden following oral challenge with tissue cysts of Me49 T. gondii strain. The highest level of protection was achieved by the mixture of rTgPI-1 and rROP2 proteins with an average parasite burden reduction of 50% compared to the unvaccinated control group. The vaccine-induced protective effect was related to the elicitation of systemic cellular and humoral immune responses that included antigen-specific spleen cell proliferation, the release of Th1/Th2 cytokines, and the generation of antigen-specific antibodies in serum. Additionally, mucosal immune responses were also induced, characterized by secretion of antigen-specific IgA antibodies in intestinal lavages and specific mesenteric lymph node cell proliferation. Our results demonstrate that rTgPI-1+rROP2 antigens seem a promising mixture to be combined with other immunogenic proteins in a multiantigenic vaccine formulation against toxoplasmosis. |
[Mh] Termos MeSH primário: |
Antígenos de Protozoários/imunologia Vacinas Protozoárias/normas Toxoplasma/imunologia Toxoplasmose Animal/prevenção & controle
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[Mh] Termos MeSH secundário: |
Animais Anticorpos Antiprotozoários/sangue Linhagem Celular Doença Crônica Citocinas/metabolismo Feminino Fibroblastos/parasitologia Prepúcio do Pênis/citologia Seres Humanos Imunoglobulina A Secretora/análise Imunoglobulina G/sangue Mucosa Intestinal/imunologia Masculino Proteínas de Membrana/imunologia Camundongos Camundongos Endogâmicos C3H Proteínas de Protozoários/imunologia Baço/citologia Baço/imunologia Vacinas Sintéticas/normas
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Cytokines); 0 (GRA4 protein, Toxoplasma gondii); 0 (Immunoglobulin A, Secretory); 0 (Immunoglobulin G); 0 (Membrane Proteins); 0 (Protozoan Proteins); 0 (Protozoan Vaccines); 0 (ROP 2 protein, Toxoplasma gondii); 0 (TgPI protein, Toxoplasma gondii); 0 (Vaccines, Synthetic) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180214 |
[Lr] Data última revisão:
| 180214 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 180109 |
[St] Status: | MEDLINE |
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