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Pesquisa : D12.776.831 [Categoria DeCS]
Referências encontradas : 590 [refinar]
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[PMID]:29197624
[Au] Autor:Fernández ML; Quartino PY; Arce-Bejarano R; Fernández J; Camacho LF; Gutiérrez JM; Kuemmel D; Fidelio G; Lomonte B
[Ad] Endereço:Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica San José 11501, Costa Rica.
[Ti] Título:Intravascular hemolysis induced by phospholipases A from the venom of the Eastern coral snake, Micrurus fulvius: Functional profiles of hemolytic and non-hemolytic isoforms.
[So] Source:Toxicol Lett;286:39-47, 2018 Apr.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A unique feature of the venom of Micrurus fulvius (Eastern coral snake) is its ability to induce severe intravascular hemolysis in particular species, such as dogs or mice. This effect was previously shown to be induced by distinct phospholipase A (PLA ) isoforms which cause direct hemolysis in vitro, an uncommon finding for such enzymes. The functional profiles of PLA -17, a direct hemolytic enzyme, and PLA -12, a co-existing venom isoform lacking such effect, were compared. The enzymes differed not only in their ability to cause intravascular hemolysis: PLA -17 additionally displayed lethal, myotoxic, and anticoagulant actions, whereas PLA -12 lacked these effects. PLA -12 was much more active in hydrolyzing a monodisperse synthetic substrate than PLA -17, but the catalytic activity of latter was notably higher on a micellar substrate, or towards pure phospholipid artificial monolayers under controlled lateral pressures. Interestingly, PLA -17 could hydrolyze substrate at a pressure of 20 mN m , in contrast to PLA -12 or the non-toxic pancreatic PLA . This suggests important differences in the monolayer penetrating power, which could be related to differences in toxicity. Comparative examination of primary structures and predicted three-dimensional folding of PLA -12 and PLA -17, revealed that differences concentrate in their N-terminal and central regions, leading to variations of the surface properties at the membrane interacting interface. PLA -17 presents a less basic interfacial surface than PLA -12, but more bulky aromatic residues, which could be associated to its higher membrane-penetrating strength. Altogether, these structural and functional comparative observations suggest that the ability of PLA s to penetrate substrate interfaces could be a major determinant of toxicity, perhaps more important than protein surface charge.
[Mh] Termos MeSH primário: Cobras Corais
Venenos Elapídicos/toxicidade
Hemólise/efeitos dos fármacos
Fosfolipases A2/toxicidade
Proteínas de Répteis/toxicidade
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Venenos Elapídicos/enzimologia
Feminino
Masculino
Camundongos
Modelos Moleculares
Permeabilidade
Fosfolipases A2/química
Fosfolipases A2/metabolismo
Conformação Proteica
Dobramento de Proteína
Isoformas de Proteínas
Proteínas de Répteis/química
Proteínas de Répteis/metabolismo
Relação Estrutura-Atividade
Propriedades de Superfície
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Elapid Venoms); 0 (Protein Isoforms); 0 (Reptilian Proteins); 0 (micrurus venom); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE


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[PMID]:27773569
[Au] Autor:Leal F; Cohn MJ
[Ad] Endereço:Howard Hughes Medical Institute, UF Genetics Institute, University of Florida, P.O. Box 103610, University of Florida, Gainesville, FL 32610, USA; Department of Biology, UF Genetics Institute, University of Florida, P.O. Box 103610, University of Florida, Gainesville, FL 32610, USA.
[Ti] Título:Loss and Re-emergence of Legs in Snakes by Modular Evolution of Sonic hedgehog and HOXD Enhancers.
[So] Source:Curr Biol;26(21):2966-2973, 2016 Nov 07.
[Is] ISSN:1879-0445
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Limb reduction and loss are hallmarks of snake evolution. Although advanced snakes are completely limbless, basal and intermediate snakes retain pelvic girdles and small rudiments of the femur. Moreover, legs may have re-emerged in extinct snake lineages [1-5], suggesting that the mechanisms of limb development were not completely lost in snakes. Here we report that hindlimb development arrests in python embryos as a result of mutations that abolish essential transcription factor binding sites in the limb-specific enhancer of Sonic hedgehog (SHH). Consequently, SHH transcription is weak and transient in python hindlimb buds, leading to early termination of a genetic circuit that drives limb outgrowth. Our results suggest that degenerate evolution of the SHH limb enhancer played a role in reduction of hindlimbs during snake evolution. By contrast, HOXD digit enhancers are conserved in pythons, and HOXD gene expression in the hindlimb buds progresses to the distal phase, forming an autopodial (digit) domain. Python hindlimb buds then develop transitory pre-chondrogenic condensations of the tibia, fibula, and footplate, raising the possibility that re-emergence of hindlimbs during snake evolution did not require de novo re-evolution of lost structures but instead could have resulted from persistence of embryonic legs. VIDEO ABSTRACT.
[Mh] Termos MeSH primário: Padronização Corporal
Boidae/genética
Evolução Molecular
Extremidades/crescimento & desenvolvimento
Regulação da Expressão Gênica no Desenvolvimento
Proteínas de Répteis/genética
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Boidae/anatomia & histologia
Boidae/crescimento & desenvolvimento
Extremidades/anatomia & histologia
Proteínas Hedgehog/genética
Proteínas Hedgehog/metabolismo
Proteínas de Répteis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hedgehog Proteins); 0 (Reptilian Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28760718
[Au] Autor:Merchant M; Morkotinis V; Hale A; White M; Moran C
[Ad] Endereço:Department of Chemistry, McNeese State University, Lake Charles, LA, USA. Electronic address: mmerchant@mcneese.edu.
[Ti] Título:Crocodylian nuclear factor kappa B.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;213:28-34, 2017 Nov.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We deduced the amino acid (aa) sequence of the nuclear factor kappa B (NFκB) protein from genomic data for the American alligator (Alligator mississippiensis), the estuarine crocodile (Crocodylus porosus), and the Indian gharial (Gavialis gangeticus). A 105kDa protein, NFκB1 exhibits complex post-translational processing, multiple mechanisms of activation, and acts as precursor for a p50, a Rel homology transcription factor which influences the expression of key genes for developmental processes, apoptosis, and immune function. The aa sequences of the crocodylian proteins share very high identity with each other (97.2±0.7%), birds (81.0±1.1%, n=6), mammals (75.3±1.6%, n=4), reptiles (80.3±5.1%, n=2), and less identity with fish (55.5±5.5%, n=4) and one amphibian (66.1±0.8%). The crocodylian protein has a well-conserved Rel homology domain, a nuclear localization signal, and a glycine-rich region which facilitates proteasome-mediated generation of p50. The Rel homology domain contains sequences responsible for dimerization, DNA-binding, and nuclear translocation. In addition, seven ankyrin repeats were located, which putatively allow for inhibition of transcriptional regulation by mediating interaction with Inhibitor kappa B. Other features include a death domain, and conserved serine residues, near the C-terminal end, which act as potential phosphorylation sites for activation of the proteolytic generation of p50. Western blot analysis showed both the 105kDa precursor and the 50kDa mature NFκB were expressed in the alligator liver. Nuclear factor κB exhibited diffuse cytoplasmic distribution in alligator hepatocytes, and almost no cytoplasmic localization in infected animals. In addition, nuclear NFκB exhibited specific binding to the consensus NFκB promoter element.
[Mh] Termos MeSH primário: Jacarés e Crocodilos
Regulação da Expressão Gênica/fisiologia
Subunidade p50 de NF-kappa B
Proteínas de Répteis
[Mh] Termos MeSH secundário: Jacarés e Crocodilos/genética
Jacarés e Crocodilos/metabolismo
Animais
Núcleo Celular/genética
Núcleo Celular/metabolismo
Citoplasma/genética
Citoplasma/metabolismo
Subunidade p50 de NF-kappa B/genética
Subunidade p50 de NF-kappa B/metabolismo
Especificidade de Órgãos/fisiologia
Proteínas de Répteis/genética
Proteínas de Répteis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (NF-kappa B p50 Subunit); 0 (Reptilian Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE


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[PMID]:28759578
[Au] Autor:Vieira SM; da Rocha SLG; Neves-Ferreira AGDC; Almeida RV; Perales J
[Ad] Endereço:Laboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
[Ti] Título:Heterologous expression of the antimyotoxic protein DM64 in Pichia pastoris.
[So] Source:PLoS Negl Trop Dis;11(7):e0005829, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Snakebite envenomation is a neglected condition that constitutes a public health problem in tropical and subtropical countries, including Brazil. Interestingly, some animals are resistant to snake envenomation due to the presence of inhibitory glycoproteins in their serum that target toxic venom components. DM64 is an acidic glycoprotein isolated from Didelphis aurita (opossum) serum that has been characterized as an inhibitor of the myotoxicity induced by bothropic toxins bearing phospholipase A2 (PLA2) structures. This antitoxic protein can serve as an excellent starting template for the design of novel therapeutics against snakebite envenomation, particularly venom-induced local tissue damage. Therefore, the aim of this work was to produce a recombinant DM64 (rDM64) in the methylotrophic yeast Pichia pastoris and to compare its biological properties with those of native DM64. Yeast fermentation in the presence of Pefabloc, a serine protease inhibitor, stimulated cell growth (~1.5-fold), increased the rDM64 production yield approximately 10-fold and significantly reduced the susceptibility of rDM64 to proteolytic degradation. P. pastoris fermentation products were identified by mass spectrometry and Western blotting. The heterologous protein was efficiently purified from the culture medium by affinity chromatography (with immobilized PLA2 myotoxin) and/or an ion exchange column. Although both native and recombinant DM64 exhibit different glycosylation patterns, they show very similar electrophoretic mobilities after PNGase F treatment. rDM64 formed a noncovalent complex with myotoxin II (Lys49-PLA2) from Bothrops asper and displayed biological activity that was similar to that of native DM64, inhibiting the cytotoxicity of myotoxin II by 92% at a 1:1 molar ratio.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/química
Inibidores de Fosfolipase A2/química
Fosfolipases A2/química
Proteínas de Répteis/química
Venenos de Serpentes/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas Sanguíneas/biossíntese
Bothrops
Brasil
Linhagem Celular
Espectrometria de Massas
Camundongos
Gambás
Pichia
Proteínas Recombinantes/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (DM64 protein, Didelphis marsupialis); 0 (Phospholipase A2 Inhibitors); 0 (Recombinant Proteins); 0 (Reptilian Proteins); 0 (Snake Venoms); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170827
[Lr] Data última revisão:
170827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005829


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[PMID]:28729066
[Au] Autor:Liu L; Jiang G; Peng Z; Li Y; Li J; Zou L; He Z; Wang X; Chu W
[Ad] Endereço:College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China; Fisheries Research Institute of Hunan Province, Changsha 410153, China.
[Ti] Título:The effect of high fat diet on daily rhythm of the core clock genes and muscle functional genes in the skeletal muscle of Chinese soft-shelled turtle (Trionyx sinensis).
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;213:17-27, 2017 Nov.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the present study, we sought to investigate the influence of high fat diet on the core clock genes and the muscle functional genes daily expression in the skeletal muscle of Chinese soft-shelled turtle. The turtles were fed by two diets including a control fat diet (the CON treatment, 7.98% lipid) and a high fat diet (the HFD treatment, 13.86% lipid) for six weeks and administrated by the photophase regimen of 24h light/dark (12L:12D) cycle. After the feeding trial experiment, we measured the daily expression levels of 17 core clock genes (Clock, Bmal1/2, NPAS2, Tim, Cry1/2, Per1/2, DBP, AANAT, NIFL3, BHLHE40, NR1D2, RORA, RORB, RORC) and 12 muscle functional genes (FBXO32, MBNL1, MSTN, Myf5, Myf6, MyoD, MyoG, MyoM1, PPARa, PDK4, Trim63, UCP3) in the skeletal muscle of the two treatments. The results showed that except for Bmal1, NPAS2, Per2 and RORB, the expression of the other 13 core clock genes exhibited circadian oscillation in the CON treatment. Among the 12 muscle functional genes, MBNL1, PDK4 and MyoM1 did not exhibit circadian oscillation in the CON treatment. In the HFD treatment, the circadian rhythms expressional patterns of the 8 core clock genes (Clock, Bmal2, Cry2, Per1, DBP, NFIL3, BHLHE40 and RORA) and 6 muscle functional genes (MSTN, Myf5, MyoD, MyoG, PPARa and Trim63) were disrupted. In addition, compared with the CON treatment, the circadian expression of the 5 core clock genes (Tim, Cry1, AANAT, NR1D2, RORC) and the 3 muscle functional genes (FBXO32, Myf6, UCP3) showed the advanced or delayed expression peaks in the HFD treatment. In CON treatment, the circadian expression of the MyoG, MyoD, Myf6, FBXO32 and PPARa showed positive or negative correlation with the transcription pattern of Clock, Bmal2, Cry1/2, Per1/2. However, only the FBXO32 and Myf6 presented positive or negative correlation with the circadian expression of Cry1, RORB, AANAT and Tim in HFD treatment. In summary, these results demonstrate that the disruption of the circadian rhythm of the core clock genes and muscle functional genes in the skeletal muscle is closely associated with feeding high fat diet to Chinese soft-shelled turtle.
[Mh] Termos MeSH primário: Relógios Circadianos/efeitos dos fármacos
Gorduras na Dieta/farmacologia
Proteínas Musculares/biossíntese
Músculo Esquelético/metabolismo
Proteínas Circadianas Period/biossíntese
Proteínas de Répteis/biossíntese
Tartarugas/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Musculares/genética
Proteínas Circadianas Period/genética
Proteínas de Répteis/genética
Tartarugas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats); 0 (Muscle Proteins); 0 (Period Circadian Proteins); 0 (Reptilian Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE


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[PMID]:28719870
[Au] Autor:Cocci P; Capriotti M; Mosconi G; Palermo FA
[Ad] Endereço:School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032 Camerino, MC, Italy. Electronic address: paolo.cocci@unicam.it.
[Ti] Título:Effects of endocrine disrupting chemicals on estrogen receptor alpha and heat shock protein 60 gene expression in primary cultures of loggerhead sea turtle (Caretta caretta) erythrocytes.
[So] Source:Environ Res;158:616-624, 2017 10.
[Is] ISSN:1096-0953
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The loggerhead turtle (Caretta caretta) can be considered a good indicator species for studying the ecological impact of endocrine disrupting chemicals (EDCs) on wildlife. However, the effect of these environmental pollutants on nuclear steroid hormone signaling has not yet been addressed in sea turtles mainly due to the legal constraints of their endangered status. Here we describe the use of primary erythrocyte cell cultures as in vitro models for evaluating the effects of different EDCs on the expression of estrogen receptor α (ERα). In addition, we evaluated erythrocyte toxicity caused by EDCs using Alamar Blue assay and heat shock proteins 60 (HSP60) expression. Primary cultures of erythrocytes were exposed to increasing concentrations of 4-nonylphenol (4NP), Diisodecyl phthalate (DiDP), Tri-m-cresyl phosphate (TMCP) and Tributyltin (TBT) for 48h. Alamar Blue demonstrated that exposure of erythrocytes to each contaminant for up to 48h led to a significant impairment of cellular metabolic activity at 100µM, with the exception of TBT. Moreover, our data indicate that loggerhead erythrocytes constitutively express ERα and HSP60 at the transcript level and respond to EDCs by up-regulating their expression. In this regard, ERα was up-regulated in a dose-dependent manner after 48h exposure to both 4NP and TMCP. Interestingly, the dosage-dependent effects of DiDP on ERα expression were opposite in comparison to that obtained following exposure to the other tested compounds. This work provides the first indication regarding the potential of primary erythrocytes as study models for evaluating the effects of EDCs on sea turtles.
[Mh] Termos MeSH primário: Chaperonina 60/genética
Disruptores Endócrinos/toxicidade
Eritrócitos/efeitos dos fármacos
Receptor alfa de Estrogênio/genética
Regulação da Expressão Gênica/efeitos dos fármacos
Proteínas de Répteis/genética
Tartarugas/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Chaperonina 60/metabolismo
Receptor alfa de Estrogênio/metabolismo
Proteínas de Répteis/metabolismo
Tartarugas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chaperonin 60); 0 (Endocrine Disruptors); 0 (Estrogen Receptor alpha); 0 (Reptilian Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE


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[PMID]:28599627
[Au] Autor:Baier F; Schmitz A; Sauer-Gürth H; Wink M
[Ad] Endereço:Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69121, Heidelberg, Germany. fbaier@fas.harvard.edu.
[Ti] Título:Pre-Quaternary divergence and subsequent radiation explain longitudinal patterns of genetic and morphological variation in the striped skink, Heremites vittatus.
[So] Source:BMC Evol Biol;17(1):132, 2017 Jun 09.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Many animal and plant species in the Middle East and northern Africa have a predominantly longitudinal distribution, extending from Iran and Turkey along the eastern Mediterranean coast into northern Africa. These species are potentially characterized by longitudinal patterns of biological diversity, but little is known about the underlying biogeographic mechanisms and evolutionary timescales. We examined these questions in the striped skink, Heremites vittatus, one such species with a roughly longitudinal distribution across the Middle East and northern Africa, by analyzing range-wide patterns of mitochondrial DNA (mtDNA) sequence and multi-trait morphological variation. RESULTS: The striped skink exhibits a basic longitudinal organization of mtDNA diversity, with three major mitochondrial lineages inhabiting northern Africa, the eastern Mediterranean coast, and Turkey/Iran. Remarkably, these lineages are of pre-Quaternary origin, and are characterized by p-distances of 9-10%. In addition, within each of these lineages a more recent Quaternary genetic diversification was observed, as evidenced by deep subclades and high haplotype diversity especially in the Turkish/Iranian and eastern Mediterranean lineages. Consistent with the genetic variation, our morphological analysis revealed that the majority of morphological traits show significant mean differences between specimens from northern Africa, the eastern Mediterranean coast, and Turkey/Iran, suggesting lineage-specific trait evolution. In addition, a subset of traits exhibits clinal variation along the eastern Mediterranean coast, potentially indicating selection gradients at the geographic transition from northern Africa to Anatolia. The existence of allopatric, morphologically and genetically divergent lineages suggests that Heremites vittatus might represent a complex with several taxa. CONCLUSIONS: Our work demonstrates that early divergence events in the Pliocene, likely driven by both climatic and geological factors, established the longitudinal patterns and distribution of Heremites vittatus. Subsequent radiation during the Pleistocene generated the genetic and morphological diversity observed today. Our study provides further evidence that longitudinal diversity patterns and species distributions in the Middle East and northern Africa were shaped by complex evolutionary processes, involving the region's intricate geological history, climatic oscillations, and the presence of the Sahara.
[Mh] Termos MeSH primário: Lagartos/classificação
Lagartos/genética
[Mh] Termos MeSH secundário: África do Norte
Animais
Biodiversidade
Evolução Biológica
Citocromos b/genética
DNA Mitocondrial/genética
Variação Genética
Lagartos/anatomia & histologia
Oriente Médio
Filogenia
Filogeografia
Proteínas de Répteis/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Reptilian Proteins); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-0969-0


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[PMID]:28275208
[Au] Autor:Fox JW; Gutiérrez JM
[Ad] Endereço:Department of Microbiology, Immunology and Cancer Biology, University of Virginia School of Medicine, P.O. Box 800734, Charlottesville, VA 22908, USA. jwf8x@virginia.edu.
[Ti] Título:Understanding the Snake Venom Metalloproteinases: An Interview with Jay Fox and José María Gutiérrez.
[So] Source:Toxins (Basel);9(1), 2017 Jan 16.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Jay W. Fox and José María Gutiérrez recently finished editing a Special Issue on the topic "Snake Venom Metalloproteinases" in . The Special Issue covers a wide range of topics, including the molecular evolution and structure of snake venom metalloproteinases (SVMPs), the mechanisms involved in the generation of diversity of SVMPs, the mechanism of action of SVMPs, and their role in the pathophysiology of envenomings, with implications for improving the therapy of envenomings. In this interview, we discussed with Jay W. Fox and José María Gutiérrez their research on the SVMPs and their perspectives on the future trends and challenges for studying snake venoms.
[Mh] Termos MeSH primário: Pesquisa Biomédica
Metaloproteases/metabolismo
Proteínas de Répteis/metabolismo
Mordeduras de Serpentes/enzimologia
Venenos de Serpentes/enzimologia
[Mh] Termos MeSH secundário: Pesquisa Biomédica/história
Escolha da Profissão
História do Século XX
História do Século XXI
Seres Humanos
Metaloproteases/história
Proteínas de Répteis/história
Mordeduras de Serpentes/história
Venenos de Serpentes/história
[Pt] Tipo de publicação:BIOGRAPHY; EDITORIAL; HISTORICAL ARTICLE; INTERVIEW; PORTRAITS
[Ps] Nome de pessoa como assunto:Fox J; Gutierrez JM
[Nm] Nome de substância:
0 (Reptilian Proteins); 0 (Snake Venoms); EC 3.4.- (Metalloproteases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE


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[PMID]:28109839
[Au] Autor:Ecay TW; Stewart JR; Wiessner G; Heulin B
[Ad] Endereço:Department of Biomedical Science, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA. Electronic address: ecay@etsu.edu.
[Ti] Título:Ex utero culture of viviparous embryos of the lizard, Zootoca vivipara, provides insights into calcium homeostasis during development.
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;206:63-68, 2017 Apr.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The chorioallantoic membrane resides adjacent to either the inner surface of the egg shell or uterine epithelium in oviparous and viviparous reptiles, respectively. Chorionic cells face the shell or uterine epithelium and transport calcium to underlying embryonic capillaries. Calcium transport activity of the chorioallantois increases in the final stages of development coincident with rapid embryonic growth and skeletal ossification. We excised embryos from viviparous Zootoca vivipara females at a stage prior to significant calcium accumulation and incubated them ex utero with and without calcium to test the hypothesis that chorioallantois calcium transport activity depends on developmental stage and not calcium availability. We measured calcium uptake by monitoring incubation media calcium content and chorioallantois expression of calbindin-D , a marker for transcellular calcium transport. The pattern of calcium flux to the media differed by incubation condition. Eggs in 0mM calcium exhibited little variation in calcium gain or loss. For eggs in 2mM calcium, calcium flux to the media was highly variable and was directed inward during the last 3days of the experiment such that embryos gained calcium. Calbindin-D expression increased under both incubation conditions but was significantly higher in embryos incubated with 2mM calcium. We conclude that embryos respond to calcium availability, yet significant calcium accumulation is developmental stage dependent. These observations suggest the chorioallantois exhibits a degree of functional plasticity that facilitates response to metabolic or environmental fluctuations.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Embrião não Mamífero/metabolismo
Desenvolvimento Embrionário
Homeostase
Lagartos/embriologia
[Mh] Termos MeSH secundário: Animais
Transporte Biológico
Biomarcadores/metabolismo
Calbindina 1/genética
Calbindina 1/metabolismo
Sinalização do Cálcio
Membrana Corioalantoide/metabolismo
Técnicas de Cultura Embrionária/veterinária
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Lagartos/metabolismo
Gravidez
Proteínas de Répteis/genética
Proteínas de Répteis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Calbindin 1); 0 (Reptilian Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE


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[PMID]:28109837
[Au] Autor:Jensen FB; Kolind RA; Jensen NS; Montesanti G; Wang T
[Ad] Endereço:Department of Biology, University of Southern Denmark, DK-5230, Odense M, Denmark. Electronic address: fbj@biology.sdu.dk.
[Ti] Título:Interspecific variation and plasticity in hemoglobin nitrite reductase activity and its correlation with oxygen affinity in vertebrates.
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;206:47-53, 2017 Apr.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deoxygenated hemoglobin (Hb) is a nitrite reductase that reduces naturally occurring nitrite to nitric oxide (NO), supplying physiological relevant NO under hypoxic conditions. The nitrite reductase activity is modulated by the allosteric equilibrium between the R and T structures of Hb that also determines oxygen affinity. In the present study we investigated nitrite reductase activity and O affinity in Hbs from ten different vertebrate species under identical conditions to disclose interspecific variations and allow an extended test for a correlation between the rate constant for nitrite reduction and O affinity. We also tested plastic changes in Hb properties via addition of T-structure-stabilizing organic phosphates (ATP and GTP). The decay in deoxyHb during its reaction with nitrite was exponential-like in ectotherms (Atlantic hagfish, carp, crucian carp, brown trout, rainbow trout, cane toad, Indian python and red-eared slider turtle), while it was sigmoid in mammals (harbor porpoise and rabbit). Typically, hypoxia-tolerant species showed a faster reaction than intolerant species. Addition of ATP and GTP decreased O affinity and slowed the rate of nitrite reduction in a concentration-dependent manner. The initial second order rate constant of the deoxyHb-mediated nitrite reduction showed a strong curvilinear correlation with oxygen affinity among all ectothermic vertebrates, and the relationship also applied to plastic variations of Hb properties via organic phosphates. The relationship predicts high nitrite reductase activity in hypoxic tolerant species with high Hb-O affinity and reveals that the decrease in erythrocyte ATP and/or GTP during acclimation to hypoxia in ectotherms increases the erythrocyte NO generating capacity.
[Mh] Termos MeSH primário: Proteínas de Anfíbios/metabolismo
Proteínas de Peixes/metabolismo
Hemoglobinas/metabolismo
Nitrito Redutases/metabolismo
Oxigênio/metabolismo
Proteínas de Répteis/metabolismo
[Mh] Termos MeSH secundário: Aclimatação
Trifosfato de Adenosina/metabolismo
Animais
Anuros/fisiologia
Boidae/fisiologia
Hipóxia Celular
Peixes
Guanosina Trifosfato/metabolismo
Cinética
Óxido Nítrico/metabolismo
Nitritos/metabolismo
Oxirredução
Phocoena
Coelhos
Especificidade da Espécie
Tartarugas/fisiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amphibian Proteins); 0 (Fish Proteins); 0 (Hemoglobins); 0 (Nitrites); 0 (Reptilian Proteins); 31C4KY9ESH (Nitric Oxide); 86-01-1 (Guanosine Triphosphate); 8L70Q75FXE (Adenosine Triphosphate); 9008-02-0 (deoxyhemoglobin); EC 1.7.- (Nitrite Reductases); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE



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