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[PMID]:29364903
[Au] Autor:Sala A; Cabassi CS; Santospirito D; Polverini E; Flisi S; Cavirani S; Taddei S
[Ad] Endereço:Department of Veterinary Science, University of Parma, Parma, Italy.
[Ti] Título:Novel Naja atra cardiotoxin 1 (CTX-1) derived antimicrobial peptides with broad spectrum activity.
[So] Source:PLoS One;13(1):e0190778, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Naja atra subsp. atra cardiotoxin 1 (CTX-1), produced by Chinese cobra snakes, belonging to Elapidae family, is included in the three-finger toxin family and exerts high cytotoxicity and antimicrobial activity too. Using as template mainly the tip and the subsequent ß-strand of the first "finger" of this toxin, different sequences of 20 amino acids linear peptides have been designed in order to avoid toxic effects but to maintain or even strengthen the partial antimicrobial activity already seen for the complete toxin. As a result, the sequence NCP-0 (Naja Cardiotoxin Peptide-0) was designed as ancestor and subsequently 4 other variant sequences of NCP-0 were developed. These synthesized variant sequences have shown microbicidal activity towards a panel of reference and field strains of Gram-positive and Gram-negative bacteria. The sequence named NCP-3, and its variants NCP-3a and NCP-3b, have shown the best antimicrobial activity, together with low cytotoxicity against eukaryotic cells and low hemolytic activity. Bactericidal activity has been demonstrated by minimum bactericidal concentration (MBC) assay at values below 10 µg/ml for most of the tested bacterial strains. This potent antimicrobial activity was confirmed even for unicellular fungi Candida albicans, Candida glabrata and Malassezia pachydermatis (MBC 50-6.3 µg/ml), and against the fast-growing mycobacteria Mycobacterium smegmatis and Mycobacterium fortuitum. Moreover, NCP-3 has shown virucidal activity on Bovine Herpesvirus 1 (BoHV1) belonging to Herpesviridae family. The bactericidal activity is maintained even in a high salt concentration medium (125 and 250 mM NaCl) and phosphate buffer with 20% Mueller Hinton (MH) medium against E. coli, methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa reference strains. Considering these in vitro obtained data, the search for active sequences within proteins presenting an intrinsic microbicidal activity could provide a new way for discovering a large number of novel and promising antimicrobial peptides families.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Proteínas Cardiotóxicas de Elapídeos/química
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anti-Infecciosos/química
Candida/efeitos dos fármacos
Bovinos
Dicroísmo Circular
Hemólise/efeitos dos fármacos
Herpesvirus Bovino 1/efeitos dos fármacos
Malassezia/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Mycobacterium/efeitos dos fármacos
Naja naja
Peptídeos/química
Conformação Proteica
Ovinos
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Cobra Cardiotoxin Proteins); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190778


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[PMID]:28854256
[Au] Autor:Debaud C; Salga M; Begot L; Holy X; Chedik M; de l'Escalopier N; Torossian F; Levesque JP; Lataillade JJ; Le Bousse-Kerdilès MC; Genêt F
[Ad] Endereço:Spine Division Orthopaedic Surgery Department, Hôpital Européen Georges Pompidou, APHP, Paris, France.
[Ti] Título:Peripheral denervation participates in heterotopic ossification in a spinal cord injury model.
[So] Source:PLoS One;12(8):e0182454, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We previously reported the development of a new acquired neurogenic HO (NHO) mouse model, combining spinal cord transection (SCI) and chemical muscle injury. Pathological mechanisms responsible for ectopic osteogenesis after central neurological damage are still to be elucidated. In this study, we first hypothesized that peripheral nervous system (PNS) might convey pathological signals from injured spinal cord to muscles in NHO mouse model. Secondly, we sought to determine whether SCI could lead to intramuscular modifications of BMP2 signaling pathways. Twenty one C57Bl6 mice were included in this protocol. Bilateral cardiotoxin (CTX) injection in hamstring muscles was associated with a two-stage surgical procedure, combining thoracic SCI with unilateral peripheral denervation. Volumes of HO (Bone Volume, BV) were measured 28 days after surgery using micro-computed tomography imaging techniques and histological analyses were made to confirm intramuscular osteogenesis. Volume comparisons were conducted between right and left hind limb of each animal, using a Wilcoxon signed rank test. Quantitative polymerase chain reaction (qPCR) was performed to explore intra muscular expression of BMP2, Alk3 and Id1. Nineteen mice survive the complete SCI and peripheral denervation procedure. When CTX injections were done right after surgery (n = 7), bilateral HO were detected in all animals after 28 days. Micro-CT measurements showed significantly increased BV in denervated paws (1.47 mm3 +/- 0.5) compared to contralateral sides (0.56 mm3 +/-0.4), p = 0.03. When peripheral denervation and CTX injections were performed after sham SCI surgery (n = 6), bilateral HO were present in three mice at day 28. Quantitative PCR analyses showed no changes in intra muscular BMP2 expression after SCI as compared to control mice (shamSCI). Peripheral denervation can be reliably added to spinal cord transection in NHO mouse model. This new experimental design confirms that neuro inflammatory mechanisms induced by central or peripheral nervous system injury plays a key role in triggering ectopic osteogenesis.
[Mh] Termos MeSH primário: Músculos/patologia
Ossificação Heterotópica/patologia
Traumatismos da Medula Espinal/patologia
Medula Espinal/patologia
[Mh] Termos MeSH secundário: Animais
Proteína Morfogenética Óssea 2/análise
Proteínas Cardiotóxicas de Elapídeos
Denervação
Modelos Animais de Doenças
Feminino
Camundongos Endogâmicos C57BL
Músculos/efeitos dos fármacos
Músculos/inervação
Ossificação Heterotópica/induzido quimicamente
Ossificação Heterotópica/diagnóstico por imagem
Ossificação Heterotópica/etiologia
Medula Espinal/diagnóstico por imagem
Medula Espinal/efeitos dos fármacos
Traumatismos da Medula Espinal/induzido quimicamente
Traumatismos da Medula Espinal/diagnóstico por imagem
Traumatismos da Medula Espinal/etiologia
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 2); 0 (Cobra Cardiotoxin Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182454


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[PMID]:28356270
[Au] Autor:Call JA; Wilson RJ; Laker RC; Zhang M; Kundu M; Yan Z
[Ad] Endereço:Department of Medicine, University of Virginia, Charlottesville, Virginia.
[Ti] Título:Ulk1-mediated autophagy plays an essential role in mitochondrial remodeling and functional regeneration of skeletal muscle.
[So] Source:Am J Physiol Cell Physiol;312(6):C724-C732, 2017 Jun 01.
[Is] ISSN:1522-1563
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Autophagy is a conserved cellular process for degrading aggregate proteins and dysfunctional organelle. It is still debatable if autophagy and mitophagy (a specific process of autophagy of mitochondria) play important roles in myogenic differentiation and functional regeneration of skeletal muscle. We tested the hypothesis that autophagy is critical for functional regeneration of skeletal muscle. We first observed time-dependent increases (3- to 6-fold) of autophagy-related proteins (Atgs), including Ulk1, Beclin1, and LC3, along with reduced p62 expression during C2C12 differentiation, suggesting increased autophagy capacity and flux during myogenic differentiation. We then used cardiotoxin (Ctx) or ischemia-reperfusion (I/R) to induce muscle injury and regeneration and observed increases in Atgs between and in adult skeletal muscle followed by increased autophagy flux after Since Ulk1 has been shown to be essential for mitophagy, we asked if Ulk1 is critical for functional regeneration in skeletal muscle. We subjected skeletal muscle-specific knockout mice (MKO) to Ctx or I/R. MKO mice had significantly impaired recovery of muscle strength and mitochondrial protein content post-Ctx or I/R. Imaging analysis showed that MKO mice have significantly attenuated recovery of mitochondrial network at 7 and 14 days post-Ctx. These findings suggest that increased autophagy protein and flux occur during muscle regeneration and Ulk1-mediated mitophagy is critical for recovery for the mitochondrial network and hence functional regeneration.
[Mh] Termos MeSH primário: Proteína Homóloga à Proteína-1 Relacionada à Autofagia/genética
Mitocôndrias/metabolismo
Degradação Mitocondrial/fisiologia
Músculo Esquelético/metabolismo
Regeneração/fisiologia
Traumatismo por Reperfusão/metabolismo
[Mh] Termos MeSH secundário: Animais
Autofagia/efeitos dos fármacos
Autofagia/fisiologia
Proteína Homóloga à Proteína-1 Relacionada à Autofagia/deficiência
Beclina-1/genética
Beclina-1/metabolismo
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular
Proteínas Cardiotóxicas de Elapídeos/toxicidade
Regulação da Expressão Gênica
Camundongos
Camundongos Knockout
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/patologia
Degradação Mitocondrial/efeitos dos fármacos
Força Muscular/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/patologia
Mioblastos Esqueléticos/efeitos dos fármacos
Mioblastos Esqueléticos/metabolismo
Mioblastos Esqueléticos/patologia
Regeneração/efeitos dos fármacos
Traumatismo por Reperfusão/genética
Traumatismo por Reperfusão/patologia
Proteína Sequestossoma-1/genética
Proteína Sequestossoma-1/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Beclin-1); 0 (Becn1 protein, mouse); 0 (Cobra Cardiotoxin Proteins); 0 (MAP1LC3 protein, mouse); 0 (Microtubule-Associated Proteins); 0 (Sequestosome-1 Protein); 0 (Sqstm1 protein, mouse); EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog); EC 2.7.11.1 (Ulk1 protein, mouse)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1152/ajpcell.00348.2016


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[PMID]:28067855
[Au] Autor:Shi YJ; Chen YJ; Hu WP; Chang LS
[Ad] Endereço:Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. a786514@gmail.com.
[Ti] Título:Detection of Naja atra Cardiotoxin Using Adenosine-Based Molecular Beacon.
[So] Source:Toxins (Basel);9(1), 2017 Jan 07.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:This study presents an adenosine (A)-based molecular beacon (MB) for selective detection of cardiotoxin (CTX) that functions by utilizing the competitive binding between CTX and the poly(A) stem of MB to coralyne. The 5'- and 3'-end of MB were labeled with a reporter fluorophore and a non-fluorescent quencher, respectively. Coralyne induced formation of the stem-loop MB structure through A2-coralyne-A2 coordination, causing fluorescence signal turn-off due to fluorescence resonance energy transfer between the fluorophore and quencher. CTX3 could bind to coralyne. Moreover, CTX3 alone induced the folding of MB structure and quenching of MB fluorescence. Unlike that of snake venom α-neurotoxins, the fluorescence signal of coralyne-MB complexes produced a bell-shaped concentration-dependent curve in the presence of CTX3 and CTX isotoxins; a turn-on fluorescence signal was noted when CTX concentration was ≤80 nM, while a turn-off fluorescence signal was noted with a further increase in toxin concentrations. The fluorescence signal of coralyne-MB complexes yielded a bell-shaped curve in response to varying concentrations of crude venom but not those of and venoms. Moreover, venom also functioned as venom to yield a bell-shaped concentration-dependent curve of MB fluorescence signal, again supporting that the hairpin-shaped MB could detect crude venoms containing CTXs. Taken together, our data validate that a platform composed of coralyne-induced stem-loop MB structure selectively detects CTXs.
[Mh] Termos MeSH primário: Adenosina/metabolismo
Alcaloides de Berberina/metabolismo
Técnicas Biossensoriais
Proteínas Cardiotóxicas de Elapídeos/metabolismo
Elapidae
Polímeros/metabolismo
[Mh] Termos MeSH secundário: Adenosina/química
Animais
Alcaloides de Berberina/química
Ligação Competitiva
Proteínas Cardiotóxicas de Elapídeos/química
Transferência Ressonante de Energia de Fluorescência
Simulação de Acoplamento Molecular
Estrutura Molecular
Polímeros/química
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Berberine Alkaloids); 0 (Cobra Cardiotoxin Proteins); 0 (Polymers); 30143-02-3 (polyadenosine); 6872-73-7 (coralyne); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170110
[St] Status:MEDLINE


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[PMID]:27984143
[Au] Autor:Gorai B; Sivaraman T
[Ad] Endereço:Structural Biology Lab, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India.
[Ti] Título:Delineating residues for haemolytic activities of snake venom cardiotoxin 1 from Naja naja as probed by molecular dynamics simulations and in vitro validations.
[So] Source:Int J Biol Macromol;95:1022-1036, 2017 Feb.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cardiotoxins (CTXs) are single polypeptide chain consisting of 59-62 amino acids with four disulfide bridges and globular proteins of simple ß-sheet folds. The CTXs are one of principal toxic components causing haemolysis and damaging various cells and belong to three-finger toxin (TFT) superfamily of snake venoms. However, there is no natural or synthetic small molecular inhibitor to the protein toxins to date. In the present study, modes of interaction of cardiotoxin 1 (CTX1) from Indian cobra (Naja naja) with heterogeneous erythrocyte membrane (EM) model system have been extensively examined by using all-atom molecular dynamics (MD) simulations in near physiological conditions and comprehensive analyses of the MD data revealed two distinct principal regions ('head groove' and 'loop groove') of the protein toxin for establishing structural interactions with the EM system. Moreover, combined analyses of data from high-throughput virtual screening of NCI small molecular database, in vitro haemolytic assays for top-hits of the chemical compounds against crude venom of Naja naja and as well CTXs purified from the venom and pharmacokinetic examinations on the chemical compounds retarding haemolytic activities of CTXs suggested that Etidronic acid and Zoledronic acid are promising prototypic chemical inhibitors to CTXs of snake venoms.
[Mh] Termos MeSH primário: Antídotos/farmacologia
Proteínas Cardiotóxicas de Elapídeos/química
Difosfonatos/farmacologia
Venenos Elapídicos/química
Ácido Etidrônico/farmacologia
Imidazóis/farmacologia
Bibliotecas de Moléculas Pequenas/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antídotos/química
Colesterol/química
Proteínas Cardiotóxicas de Elapídeos/antagonistas & inibidores
Proteínas Cardiotóxicas de Elapídeos/isolamento & purificação
Proteínas Cardiotóxicas de Elapídeos/toxicidade
Difosfonatos/química
Dissulfetos/química
Venenos Elapídicos/antagonistas & inibidores
Venenos Elapídicos/isolamento & purificação
Venenos Elapídicos/toxicidade
Elapidae/metabolismo
Membrana Eritrocítica/química
Membrana Eritrocítica/efeitos dos fármacos
Ácido Etidrônico/química
Hemólise/efeitos dos fármacos
Ensaios de Triagem em Larga Escala
Seres Humanos
Imidazóis/química
Simulação de Dinâmica Molecular
Fosfatidilcolinas/química
Fosfatidiletanolaminas/química
Fosfatidilserinas/química
Domínios Proteicos
Estrutura Secundária de Proteína
Bibliotecas de Moléculas Pequenas/química
Relação Estrutura-Atividade
Interface Usuário-Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antidotes); 0 (Cobra Cardiotoxin Proteins); 0 (Diphosphonates); 0 (Disulfides); 0 (Elapid Venoms); 0 (Imidazoles); 0 (Phosphatidylcholines); 0 (Phosphatidylethanolamines); 0 (Phosphatidylserines); 0 (Small Molecule Libraries); 10015-88-0 (1-palmitoyl-2-oleoylphosphatidylethanolamine); 6XC1PAD3KF (zoledronic acid); 70614-14-1 (1,2-dioleoylphosphatidylserine); 97C5T2UQ7J (Cholesterol); M2F465ROXU (Etidronic Acid); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


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[PMID]:27702601
[Au] Autor:Shulepko MA; Lyukmanova EN; Shenkarev ZO; Dubovskii PV; Astapova MV; Feofanov AV; Arseniev AS; Utkin YN; Kirpichnikov MP; Dolgikh DA
[Ad] Endereço:Biological Faculty, Lomonosov Moscow State University, 119234, Moscow, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya Street, 117997, Moscow, Russia.
[Ti] Título:Towards universal approach for bacterial production of three-finger Ly6/uPAR proteins: Case study of cytotoxin I from cobra N. oxiana.
[So] Source:Protein Expr Purif;130:13-20, 2017 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytotoxins or cardiotoxins is a group of polycationic toxins from cobra venom belonging to the 'three-finger' protein superfamily (Ly6/uPAR family) which includes small ß-structural proteins (60-90 residues) with high disulfide bond content (4-5 disulfides). Due to a high cytotoxic activity for cancer cells, cytotoxins are considered as potential anticancer agents. Development of the high-throughput production methods is required for the prospective applications of cytotoxins. Here, efficient approach for bacterial production of recombinant analogue of cytotoxin I from N. oxiana containing additional N-terminal Met-residue (rCTX1) was developed. rCTX1 was produced in the form of E. coli inclusion bodies. Refolding in optimized conditions provided ∼6 mg of correctly folded protein from 1 L of bacterial culture. Cytotoxicity of rCTX1 for C6 rat glioma cells was found to be similar to the activity of wild type CTX1. The milligram quantities of C, N-labeled rCTX1 were obtained. NMR study confirmed the similarity of the spatial structures of recombinant and wild-type toxins. Additional Met residue does not perturb the overall structure of the three-finger core. The analysis of available data for different Ly6/uPAR proteins of snake and human origin revealed that efficiency of their folding in vitro is correlated with the number of proline residues in the third loop and the surface area of hydrophobic residues buried within the protein interior. The obtained data indicate that hydrophobic core is important for the folding of proteins with high disulfide bond content. Developed expression method opens new possibilities for structure-function studies of CTX1 and other related three-finger proteins.
[Mh] Termos MeSH primário: Antineoplásicos
Proteínas Cardiotóxicas de Elapídeos
Elapidae/genética
Glioma/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Proteínas Cardiotóxicas de Elapídeos/biossíntese
Proteínas Cardiotóxicas de Elapídeos/genética
Proteínas Cardiotóxicas de Elapídeos/isolamento & purificação
Proteínas Cardiotóxicas de Elapídeos/farmacologia
Ensaios de Seleção de Medicamentos Antitumorais
Elapidae/metabolismo
Escherichia coli
Glioma/metabolismo
Glioma/patologia
Ressonância Magnética Nuclear Biomolecular
Estrutura Secundária de Proteína
Ratos
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cobra Cardiotoxin Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE


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[PMID]:27833743
[Au] Autor:Dinulovic I; Furrer R; Di Fulvio S; Ferry A; Beer M; Handschin C
[Ad] Endereço:Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
[Ti] Título:PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle.
[So] Source:Skelet Muscle;6:38, 2016.
[Is] ISSN:2044-5040
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Skeletal muscle tissue has an enormous regenerative capacity that is instrumental for a successful defense against muscle injury and wasting. The peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) exerts therapeutic effects in several muscle pathologies, but its role in damage-induced muscle regeneration is unclear. METHODS: Using muscle-specific gain- and loss-of-function models for PGC-1α in combination with the myotoxic agent cardiotoxin (CTX), we explored the role of this transcriptional coactivator in muscle damage and inflammation. RESULTS: Interestingly, we observed PGC-1α-dependent effects at the early stages of regeneration, in particular regarding macrophage accumulation and polarization from the pro-inflammatory M1 to the anti-inflammatory M2 type, a faster resolution of necrosis and protection against the development of fibrosis after multiple CTX-induced injuries. CONCLUSIONS: PGC-1α exerts beneficial effects on muscle inflammation that might contribute to the therapeutic effects of elevated muscle PGC-1α in different models of muscle wasting.
[Mh] Termos MeSH primário: Músculo Esquelético/patologia
Músculo Esquelético/fisiopatologia
Miosite/metabolismo
Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/fisiologia
Regeneração
[Mh] Termos MeSH secundário: Animais
Proteínas Cardiotóxicas de Elapídeos
Fibrose/metabolismo
Fibrose/fisiopatologia
Expressão Gênica
Hidroxiprolina/metabolismo
Macrófagos/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Contração Muscular
Músculo Esquelético/lesões
Músculo Esquelético/metabolismo
Miosite/induzido quimicamente
Miosite/patologia
Necrose
Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cobra Cardiotoxin Proteins); 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha); 0 (Ppargc1a protein, mouse); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161112
[St] Status:MEDLINE


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[PMID]:27306336
[Au] Autor:Joanisse S; Nederveen JP; Baker JM; Snijders T; Iacono C; Parise G
[Ad] Endereço:Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada; and.
[Ti] Título:Exercise conditioning in old mice improves skeletal muscle regeneration.
[So] Source:FASEB J;30(9):3256-68, 2016 Sep.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Skeletal muscle possesses the ability to regenerate after injury, but this ability is impaired or delayed with aging. Regardless of age, muscle retains the ability to positively respond to stimuli, such as exercise. We examined whether exercise is able to improve regenerative response in skeletal muscle of aged mice. Twenty-two-month-old male C57Bl/6J mice (n = 20) underwent an 8-wk progressive exercise training protocol [old exercised (O-Ex) group]. An old sedentary (O-Sed) and a sedentary young control (Y-Ctl) group were included. Animals were subjected to injections of cardiotoxin into the tibialis anterior muscle. The tibialis anterior were harvested before [O-Ex/O-Sed/Y-Ctl control (CTL); n = 6], 10 d (O-Ex/O-Sed/Y-Ctl d 10; n = 8), and 28 d (O-Ex/O-Sed/Y-Ctl d 28; n = 6) postinjection. Average fiber cross-sectional area was reduced in all groups at d 10 (CTL: O-Ex: 2499 ± 140; O-Sed: 2320 ± 165; Y-Ctl: 2474 ± 269; d 10: O-Ex: 1191 ± 100; O-Sed: 1125 ± 99; Y-Ctl: 1481 ± 167 µm(2); P < 0.05), but was restored to control values in O-Ex and Y-Ctl groups at d 28 (O-Ex: 2257 ± 181; Y-Ctl: 2398 ± 171 µm(2); P > 0.05). Satellite cell content was greater at CTL in O-Ex (2.6 ± 0.4 satellite cells/100 fibers) compared with O-Sed (1.0 ± 0.1% satellite cells/100 fibers; P < 0.05). Exercise conditioning appears to improve ability of skeletal muscle to regenerate after injury in aged mice.-Joanisse, S., Nederveen, J. P., Baker, J. M., Snijders, T., Iacono, C., Parise, G. Exercise conditioning in old mice improves skeletal muscle regeneration.
[Mh] Termos MeSH primário: Envelhecimento/fisiologia
Músculo Esquelético/fisiologia
Condicionamento Físico Animal/fisiologia
Regeneração/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas Cardiotóxicas de Elapídeos/toxicidade
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Resistência Física/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cobra Cardiotoxin Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201600143RR


  9 / 402 MEDLINE  
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[PMID]:27010781
[Au] Autor:Yamashita A; Hatazawa Y; Hirose Y; Ono Y; Kamei Y
[Ad] Endereço:a Graduate School of Life and Environmental Sciences , Kyoto Prefectural University , Kyoto , Japan.
[Ti] Título:FOXO1 delays skeletal muscle regeneration and suppresses myoblast proliferation.
[So] Source:Biosci Biotechnol Biochem;80(8):1531-5, 2016 Aug.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Unloading stress, such as bed rest, inhibits the regenerative potential of skeletal muscles; however, the underlying mechanisms remain largely unknown. FOXO1 expression, which induces the upregulated expression of the cell cycle inhibitors p57 and Gadd45α, is known to be increased in the skeletal muscle under unloading conditions. However, there is no report addressing FOXO1-induced inhibition of myoblast proliferation. Therefore, we induced muscle injury by cardiotoxin in transgenic mice overexpressing FOXO1 in the skeletal muscle (FOXO1-Tg mice) and observed regeneration delay in skeletal muscle mass and cross-sectional area in FOXO1-Tg mice. Increased p57 and Gadd45α mRNA levels, and decreased proliferation capacity were observed in C2C12 myoblasts expressing a tamoxifen-inducible active form of FOXO1. These results suggest that decreased proliferation capacity of myoblasts by FOXO1 disrupts skeletal muscle regeneration under FOXO1-increased conditions, such as unloading.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/genética
Inibidor de Quinase Dependente de Ciclina p57/genética
Proteína Forkhead Box O1/genética
Músculo Esquelético/metabolismo
Mioblastos/metabolismo
Proteínas Nucleares/genética
Regeneração/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Ciclo Celular/metabolismo
Diferenciação Celular
Linhagem Celular
Proliferação Celular
Proteínas Cardiotóxicas de Elapídeos/toxicidade
Inibidor de Quinase Dependente de Ciclina p57/metabolismo
Proteína Forkhead Box O1/metabolismo
Regulação da Expressão Gênica
Elevação dos Membros Posteriores
Mecanotransdução Celular
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Músculo Esquelético/lesões
Músculo Esquelético/patologia
Mioblastos/efeitos dos fármacos
Mioblastos/patologia
Proteínas Nucleares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cdkn1c protein, mouse); 0 (Cell Cycle Proteins); 0 (Cobra Cardiotoxin Proteins); 0 (Cyclin-Dependent Kinase Inhibitor p57); 0 (Forkhead Box Protein O1); 0 (Foxo1 protein, mouse); 0 (Gadd45a protein, mouse); 0 (Nuclear Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160325
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1164585


  10 / 402 MEDLINE  
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[PMID]:26963343
[Au] Autor:Kuraoka M; Kimura E; Nagata T; Okada T; Aoki Y; Tachimori H; Yonemoto N; Imamura M; Takeda S
[Ad] Endereço:Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
[Ti] Título:Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
[So] Source:Am J Pathol;186(5):1302-12, 2016 May.
[Is] ISSN:1525-2191
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Duchenne muscular dystrophy is a lethal X-linked muscle disorder. We have already reported that osteopontin (OPN), an inflammatory cytokine and myogenic factor, is expressed in the early dystrophic phase in canine X-linked muscular dystrophy in Japan, a dystrophic dog model. To further explore the possibility of OPN as a new biomarker for disease activity in Duchenne muscular dystrophy, we monitored serum OPN levels in dystrophic and wild-type dogs at different ages and compared the levels to other serum markers, such as serum creatine kinase, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1. Serum OPN levels in the dystrophic dogs were significantly elevated compared with those in wild-type dogs before and 1 hour after a cesarean section birth and at the age of 3 months. The serum OPN level was significantly correlated with the phenotypic severity of dystrophic dogs at the period corresponding to the onset of muscle weakness, whereas other serum markers including creatine kinase were not. Immunohistologically, OPN was up-regulated in infiltrating macrophages and developmental myosin heavy chain-positive regenerating muscle fibers in the dystrophic dogs, whereas serum OPN was highly elevated. OPN expression was also observed during the synergic muscle regeneration process induced by cardiotoxin injection. In conclusion, OPN is a promising biomarker for muscle regeneration in dystrophic dogs and can be applicable to boys with Duchenne muscular dystrophy.
[Mh] Termos MeSH primário: Músculo Esquelético/fisiologia
Distrofia Muscular de Duchenne/fisiopatologia
Osteopontina/metabolismo
Regeneração/fisiologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Biomarcadores/metabolismo
Proteínas Cardiotóxicas de Elapídeos/toxicidade
Diafragma/metabolismo
Cães
Masculino
Metaloproteinase 9 da Matriz/metabolismo
Músculo Esquelético/metabolismo
Distrofia Muscular Animal/fisiopatologia
Fenótipo
Inibidor Tecidual de Metaloproteinase-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cobra Cardiotoxin Proteins); 0 (Tissue Inhibitor of Metalloproteinase-1); 106441-73-0 (Osteopontin); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160311
[St] Status:MEDLINE



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