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Pesquisa : D12.776.835.725.868.437 [Categoria DeCS]
Referências encontradas : 620 [refinar]
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[PMID]:29220654
[Au] Autor:Guan BJ; van Hoef V; Jobava R; Elroy-Stein O; Valasek LS; Cargnello M; Gao XH; Krokowski D; Merrick WC; Kimball SR; Komar AA; Koromilas AE; Wynshaw-Boris A; Topisirovic I; Larsson O; Hatzoglou M
[Ad] Endereço:Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH 44106, USA.
[Ti] Título:A Unique ISR Program Determines Cellular Responses to Chronic Stress.
[So] Source:Mol Cell;68(5):885-900.e6, 2017 Dec 07.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The integrated stress response (ISR) is a homeostatic mechanism induced by endoplasmic reticulum (ER) stress. In acute/transient ER stress, decreased global protein synthesis and increased uORF mRNA translation are followed by normalization of protein synthesis. Here, we report a dramatically different response during chronic ER stress. This chronic ISR program is characterized by persistently elevated uORF mRNA translation and concurrent gene expression reprogramming, which permits simultaneous stress sensing and proteostasis. The program includes PERK-dependent switching to an eIF3-dependent translation initiation mechanism, resulting in partial, but not complete, translational recovery, which, together with transcriptional reprogramming, selectively bolsters expression of proteins with ER functions. Coordination of transcriptional and translational reprogramming prevents ER dysfunction and inhibits "foamy cell" development, thus establishing a molecular basis for understanding human diseases associated with ER dysfunction.
[Mh] Termos MeSH primário: Estresse do Retículo Endoplasmático
Fator de Iniciação 3 em Eucariotos/metabolismo
Fibroblastos/metabolismo
Biossíntese de Proteínas
RNA Mensageiro/biossíntese
Transcrição Genética
eIF-2 Quinase/metabolismo
[Mh] Termos MeSH secundário: Animais
Reprogramação Celular
Fator de Iniciação 3 em Eucariotos/genética
Fibroblastos/patologia
Células HEK293
Seres Humanos
Camundongos
Fases de Leitura Aberta
Fenótipo
Proteostase
Interferência de RNA
RNA Mensageiro/genética
Transdução de Sinais
Fatores de Tempo
Transfecção
eIF-2 Quinase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eukaryotic Initiation Factor-3); 0 (RNA, Messenger); EC 2.7.11.1 (PERK kinase); EC 2.7.11.1 (eIF-2 Kinase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


  2 / 620 MEDLINE  
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[PMID]:29031564
[Au] Autor:Yin JY; Zhang JT; Zhang W; Zhou HH; Liu ZQ
[Ad] Endereço:Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, PR China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, PR China. Electronic address: yinjiye@csu.edu.cn.
[Ti] Título:eIF3a: A new anticancer drug target in the eIF family.
[So] Source:Cancer Lett;412:81-87, 2018 Jan 01.
[Is] ISSN:1872-7980
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:eIF3a is the largest subunit of eIF3, which is a key player in all steps of translation initiation. During the past years, eIF3a is recognized as a proto-oncogene, which is an important discovery in this field. It is widely reported to be correlated with cancer occurrence, metastasis, prognosis, and therapeutic response. Recently, the mechanisms of eIF3a action in the carcinogenesis are unveiled gradually. A number of cellular, physiological, and pathological processes involving eIF3a are identified. Most importantly, it is emerging as a new potential drug target in the eIF family, and some small molecule inhibitors are being developed. Thus, we perform a critical review of recent advances in understanding eIF3a physiological and pathological functions, with specific focus on its role in cancer and anticancer drug targets.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Fator de Iniciação 3 em Eucariotos/fisiologia
[Mh] Termos MeSH secundário: Animais
Carcinogênese
Ciclo Celular
DNA/biossíntese
Reparo do DNA
Fator de Iniciação 3 em Eucariotos/análise
Fator de Iniciação 3 em Eucariotos/antagonistas & inibidores
Fator de Iniciação 3 em Eucariotos/química
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (EIF3A protein, human); 0 (Eukaryotic Initiation Factor-3); 9007-49-2 (DNA)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171205
[Lr] Data última revisão:
171205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


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[PMID]:28870987
[Au] Autor:Aguero T; Jin Z; Chorghade S; Kalsotra A; King ML; Yang J
[Ad] Endereço:Department of Cell Biology, University of Miami, Miami, FL 33136, USA.
[Ti] Título:Maternal Dead-end 1 promotes translation of by binding the eIF3 complex.
[So] Source:Development;144(20):3755-3765, 2017 10 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.
[Mh] Termos MeSH primário: Fator de Iniciação 3 em Eucariotos/metabolismo
Proteínas de Ligação a RNA/metabolismo
Proteínas Repressoras/metabolismo
Proteínas de Xenopus/metabolismo
Xenopus laevis
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Feminino
Fertilização
Regulação da Expressão Gênica no Desenvolvimento
Células HEK293
Seres Humanos
Camundongos
Oócitos/metabolismo
Iniciação Traducional da Cadeia Peptídica
Plasmídeos/metabolismo
Ligação Proteica
Biossíntese de Proteínas
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Dnd1 protein, Xenopus); 0 (Eukaryotic Initiation Factor-3); 0 (RNA-Binding Proteins); 0 (Repressor Proteins); 0 (Xenopus Proteins); 0 (nanos1 protein, Xenopus)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1242/dev.152611


  4 / 620 MEDLINE  
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[PMID]:28505193
[Au] Autor:Renda F; Pellacani C; Strunov A; Bucciarelli E; Naim V; Bosso G; Kiseleva E; Bonaccorsi S; Sharp DJ; Khodjakov A; Gatti M; Somma MP
[Ad] Endereço:Dipartimento di Biologia e Biotecnologie "C. Darwin", Sapienza, Università di Roma, Roma, Italy.
[Ti] Título:The Drosophila orthologue of the INT6 onco-protein regulates mitotic microtubule growth and kinetochore structure.
[So] Source:PLoS Genet;13(5):e1006784, 2017 May.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INT6/eIF3e is a highly conserved component of the translation initiation complex that interacts with both the 26S proteasome and the COP9 signalosome, two complexes implicated in ubiquitin-mediated protein degradation. The INT6 gene was originally identified as the insertion site of the mouse mammary tumor virus (MMTV), and later shown to be involved in human tumorigenesis. Here we show that depletion of the Drosophila orthologue of INT6 (Int6) results in short mitotic spindles and deformed centromeres and kinetochores with low intra-kinetochore distance. Poleward flux of microtubule subunits during metaphase is reduced, although fluorescence recovery after photobleaching (FRAP) demonstrates that microtubules remain dynamic both near the kinetochores and at spindle poles. Mitotic progression is delayed during metaphase due to the activity of the spindle assembly checkpoint (SAC). Interestingly, a deubiquitinated form of the kinesin Klp67A (a putative orthologue of human Kif18A) accumulates near the kinetochores in Int6-depleted cells. Consistent with this finding, Klp67A overexpression mimics the Int6 RNAi phenotype. Furthermore, simultaneous depletion of Int6 and Klp67A results in a phenotype identical to RNAi of just Klp67A, which indicates that Klp67A deficiency is epistatic over Int6 deficiency. We propose that Int6-mediated ubiquitination is required to control the activity of Klp67A. In the absence of this control, excess of Klp67A at the kinetochore suppresses microtubule plus-end polymerization, which in turn results in reduced microtubule flux, spindle shortening, and centromere/kinetochore deformation.
[Mh] Termos MeSH primário: Fator de Iniciação 3 em Eucariotos/genética
Cinetocoros/metabolismo
Microtúbulos/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Drosophila/genética
Drosophila/metabolismo
Drosophila/ultraestrutura
Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Fator de Iniciação 3 em Eucariotos/metabolismo
Cinetocoros/ultraestrutura
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Microtúbulos/genética
Mitose
Ubiquitinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Eukaryotic Initiation Factor-3); 0 (KLP67A protein, Drosophila); 0 (Microtubule-Associated Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006784


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[PMID]:28359406
[Au] Autor:Xu JZ; Wen F; Wang XR
[Ad] Endereço:Department of Gynaecology, Jingzhou Central Hospital, the Second Clinical Medical College, Yangtze University, Jingzhou, PR China.
[Ti] Título:The eIF3a Arg803Lys genetic polymorphism is associated with susceptibility to and chemoradiotherapy efficacy in cervical carcinoma.
[So] Source:Kaohsiung J Med Sci;33(4):187-194, 2017 Apr.
[Is] ISSN:1607-551X
[Cp] País de publicação:China (Republic : 1949- )
[La] Idioma:eng
[Ab] Resumo:We aimed to explore the correlations between eukaryotic translation initiation factor 3, subunit A (eIF3a) polymorphisms and susceptibility to and chemoradiotherapy efficacy in cervical carcinoma. Between August 2007 and August 2011, 176 patients with cervical carcinoma were enrolled as the case group, and 180 healthy individuals were selected as the control group. eIF3a Arg803Lys C>T genotypes were detected by hemi-nested polymerase chain reaction restriction fragment length polymorphism. All patients received chemoradiotherapy and were evaluated for efficacy. Compared with carriers of the CC genotype, carriers of the T genotype of the eIF3a Arg803Lys C>T polymorphism had a higher risk of cervical carcinoma. The eIF3a Arg803Lys C>T polymorphism was associated with tumor size, differentiation degree, Federation of Gynecology and Obstetrics (FIGO) stage, and lymph node metastasis (LNM). The overall response rate of the case group was 69.32% (122/176). The response rate of CC genotype carriers was higher compared to patients with the CT+TT genotypes. Binary-logistic regression analysis showed that tumor size, FIGO stage, LNM, and the eIF3a Arg803Lys C>T polymorphism were influencing factors for chemoradiotherapy efficacy. Univariate analysis revealed that age, eIF3a Arg803Lys C>T polymorphism, differentiation degree, FIGO stage, and LNM were prognostic factors of cervical carcinoma, and multivariate analysis showed that age ≥ 60 years, higher FIGO stage, and LNM, as well as the CT and TT genotypes of the eIF3a Arg803Lys C>T polymorphism, were risk factors related to the prognosis of cervical carcinoma. The eIF3a Arg803Lys C>T polymorphism is connected with a higher susceptibility to cervical carcinoma and may affect chemoradiotherapy efficacy in and prognosis of cervical carcinoma.
[Mh] Termos MeSH primário: Quimiorradioterapia
Fator de Iniciação 3 em Eucariotos/genética
Predisposição Genética para Doença
Polimorfismo de Nucleotídeo Único/genética
Neoplasias do Colo do Útero/genética
Neoplasias do Colo do Útero/terapia
[Mh] Termos MeSH secundário: Idoso
Estudos de Casos e Controles
Feminino
Frequência do Gene/genética
Seres Humanos
Estimativa de Kaplan-Meier
Modelos Logísticos
Meia-Idade
Resultado do Tratamento
Neoplasias do Colo do Útero/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (EIF3A protein, human); 0 (Eukaryotic Initiation Factor-3)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


  6 / 620 MEDLINE  
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[PMID]:28231410
[Au] Autor:Li T; Li S; Chen D; Chen B; Yu T; Zhao F; Wang Q; Yao M; Huang S; Chen Z; He X
[Ad] Endereço:Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
[Ti] Título:Transcriptomic analyses of RNA-binding proteins reveal eIF3c promotes cell proliferation in hepatocellular carcinoma.
[So] Source:Cancer Sci;108(5):877-885, 2017 May.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:RNA-binding proteins (RBPs) play fundamental roles in the RNA life cycle. The aberrant expression of RBPs is often observed in human disease, including cancer. In this study, we screened for the expression levels of 1542 human RBPs in The Cancer Genome Atlas liver hepatocellular carcinoma samples and found 92 consistently upregulated RBP genes in HCC compared with normal samples. Additionally, we undertook a Kaplan-Meier analysis and found that high expression of 15 RBP genes was associated with poor prognosis in patients with HCC. Furthermore, we found that eIF3c promotes HCC cell proliferation in vitro as well as tumorigenicity in vivo. Gene Set Enrichment Analysis showed that high eIF3c expression is positively associated with KRAS, vascular endothelial growth factor, and Hedgehog signaling pathways, all of which are closely associated with specific cancer-related gene sets. Our study provides the basis for further investigation of the molecular mechanism by which eIF3c promotes the development and progression of HCC.
[Mh] Termos MeSH primário: Carcinoma Hepatocelular/genética
Proliferação Celular/genética
Fator de Iniciação 3 em Eucariotos/genética
Neoplasias Hepáticas/genética
Proteínas de Ligação a RNA/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Carcinoma Hepatocelular/patologia
Linhagem Celular
Linhagem Celular Tumoral
Progressão da Doença
Feminino
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica/genética
Células HEK293
Seres Humanos
Estimativa de Kaplan-Meier
Neoplasias Hepáticas/patologia
Masculino
Meia-Idade
Transdução de Sinais/genética
Regulação para Cima/genética
Fator A de Crescimento do Endotélio Vascular/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (EIF3D protein, human); 0 (Eukaryotic Initiation Factor-3); 0 (RNA-Binding Proteins); 0 (Vascular Endothelial Growth Factor A)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13209


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[PMID]:28193911
[Au] Autor:Zhang Y; Wang P; Zhang Q; Yao X; Zhao L; Liu Y; Liu X; Tao R; Yu C; Li Y; Song X; Yao S
[Ad] Endereço:State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
[Ti] Título:eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation.
[So] Source:Oncotarget;8(12):19968-19979, 2017 Mar 21.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Translational control is a critical step in the regulation of gene expression. Accumulating evidence shows that translational control of a subgroup of mRNAs tends to be selective. However, our understanding of the function of selective translational control in endothelial cells is still incomplete. We found that a key translational regulator, eIF3i, is highly expressed in endothelial cells during embryonic and tumor angiogenesis. Knockdown of eIF3i restrained cell proliferation and migration in endothelial cells. In zebrafish angiogenesis model, eIF3i mutant endothelial cells could not respond to induction signals from tumor mass. Mechanistically, we showed that eIF3i knockdown reduced VEGFR/ERK signaling by down-regulating VEGFR2 and ERK protein expression. Gene therapy model suggested that the growth and metastasis of cancer cells were suppressed by eIF3i shRNA. Therefore, our work established a selective translational regulatory mechanism during tumor induced angiogenesis and suggested that targeting eIF3i may be applicable for anticancer therapy.
[Mh] Termos MeSH primário: Fator de Iniciação 3 em Eucariotos/metabolismo
Regulação Neoplásica da Expressão Gênica
Células Endoteliais da Veia Umbilical Humana/patologia
Melanoma Experimental/irrigação sanguínea
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Neovascularização Patológica/patologia
Receptores de Fatores de Crescimento do Endotélio Vascular/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose
Biomarcadores Tumorais/genética
Biomarcadores Tumorais/metabolismo
Movimento Celular
Proliferação Celular
Fator de Iniciação 3 em Eucariotos/genética
Feminino
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Melanoma Experimental/metabolismo
Melanoma Experimental/patologia
Camundongos
Camundongos Nus
Proteína Quinase 1 Ativada por Mitógeno/genética
Proteína Quinase 3 Ativada por Mitógeno/genética
Neovascularização Patológica/metabolismo
Biossíntese de Proteínas
Receptores de Fatores de Crescimento do Endotélio Vascular/genética
Células Tumorais Cultivadas
Ensaios Antitumorais Modelo de Xenoenxerto
Peixe-Zebra/embriologia
Peixe-Zebra/genética
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Eukaryotic Initiation Factor-3); 171601-26-6 (EIF3I protein, human); EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor); EC 2.7.11.24 (MAPK1 protein, human); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15274


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[PMID]:28119417
[Au] Autor:Mohammad MP; Munzarová Pondelícková V; Zeman J; Gunisová S; Valásek LS
[Ad] Endereço:Laboratory of Regulation of Gene Expression, Institute of Microbiology AS CR, Prague, Videnska 1083, 142 20, Czech Republic.
[Ti] Título:In vivo evidence that eIF3 stays bound to ribosomes elongating and terminating on short upstream ORFs to promote reinitiation.
[So] Source:Nucleic Acids Res;45(5):2658-2674, 2017 Mar 17.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Translation reinitiation is a gene-specific translational control mechanism characterized by the ability of some short upstream ORFs to prevent recycling of the post-termination 40S subunit in order to resume scanning for reinitiation downstream. Its efficiency decreases with the increasing uORF length, or by the presence of secondary structures, suggesting that the time taken to translate a uORF is more critical than its length. This led to a hypothesis that some initiation factors needed for reinitiation are preserved on the 80S ribosome during early elongation. Here, using the GCN4 mRNA containing four short uORFs, we developed a novel in vivo RNA-protein Ni2+-pull down assay to demonstrate for the first time that one of these initiation factors is eIF3. eIF3 but not eIF2 preferentially associates with RNA segments encompassing two GCN4 reinitiation-permissive uORFs, uORF1 and uORF2, containing cis-acting 5΄ reinitiation-promoting elements (RPEs). We show that the preferred association of eIF3 with these uORFs is dependent on intact RPEs and the eIF3a/TIF32 subunit and sharply declines with the extended length of uORFs. Our data thus imply that eIF3 travels with early elongating ribosomes and that the RPEs interact with eIF3 in order to stabilize the mRNA-eIF3-40S post-termination complex to stimulate efficient reinitiation downstream.
[Mh] Termos MeSH primário: Fator de Iniciação 3 em Eucariotos/metabolismo
Regulação da Expressão Gênica
Fases de Leitura Aberta
Iniciação Traducional da Cadeia Peptídica
Ribossomos/metabolismo
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Códon de Terminação
Técnicas Genéticas
Elongação Traducional da Cadeia Peptídica
Terminação Traducional da Cadeia Peptídica
Subunidades Ribossômicas Menores de Eucariotos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Codon, Terminator); 0 (Eukaryotic Initiation Factor-3)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx049


  9 / 620 MEDLINE  
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[PMID]:28013103
[Au] Autor:Le Sage V; Cinti A; McCarthy S; Amorim R; Rao S; Daino GL; Tramontano E; Branch DR; Mouland AJ
[Ad] Endereço:HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, Québec, Canada H3T 1E2.
[Ti] Título:Ebola virus VP35 blocks stress granule assembly.
[So] Source:Virology;502:73-83, 2017 Feb.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stress granules (SGs) are dynamic cytoplasmic aggregates of translationally silenced mRNAs that assemble in response to environmental stress. SGs appear to play an important role in antiviral innate immunity and many viruses have evolved to block or subvert SGs components for their own benefit. Here, we demonstrate that intracellular Ebola virus (EBOV) replication and transcription-competent virus like particles (trVLP) infection does not lead to SG assembly but leads to a blockade to Arsenite-induced SG assembly. Moreover we show that EBOV VP35 represses the assembly of canonical and non-canonical SGs induced by a variety of pharmacological stresses. This SG blockade requires, at least in part, the C-terminal domain of VP35. Furthermore, results from our co-immunoprecipitation studies indicate that VP35 interacts with multiple SG components, including G3BP1, eIF3 and eEF2 through a stress- and RNA-independent mechanism. These data suggest a novel function for EBOV VP35 in the repression of SG assembly.
[Mh] Termos MeSH primário: Grânulos Citoplasmáticos/virologia
Ebolavirus/metabolismo
Doença pelo Vírus Ebola/virologia
Proteínas Virais Reguladoras e Acessórias/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Transporte/metabolismo
Grânulos Citoplasmáticos/metabolismo
DNA Helicases
Ebolavirus/química
Ebolavirus/genética
Fator de Iniciação 2 em Eucariotos/metabolismo
Fator de Iniciação 3 em Eucariotos/metabolismo
Doença pelo Vírus Ebola/metabolismo
Interações Hospedeiro-Patógeno
Seres Humanos
Proteínas de Ligação a Poli-ADP-Ribose
Ligação Proteica
Domínios Proteicos
RNA Helicases
Proteínas com Motivo de Reconhecimento de RNA
Proteínas Virais Reguladoras e Acessórias/química
Proteínas Virais Reguladoras e Acessórias/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Eukaryotic Initiation Factor-2); 0 (Eukaryotic Initiation Factor-3); 0 (Poly-ADP-Ribose Binding Proteins); 0 (RNA Recognition Motif Proteins); 0 (VP35 protein, filovirus); 0 (Viral Regulatory and Accessory Proteins); EC 3.6.4.- (DNA Helicases); EC 3.6.4.12 (G3BP1 protein, human); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE


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[PMID]:28007963
[Au] Autor:Parasuraman P; Mulligan P; Walker JA; Li B; Boukhali M; Haas W; Bernards A
[Ad] Endereço:From the Massachusetts General Hospital Center for Cancer Research and Harvard Medical School, Charlestown, Massachusetts 02129.
[Ti] Título:Interaction of p190A RhoGAP with eIF3A and Other Translation Preinitiation Factors Suggests a Role in Protein Biosynthesis.
[So] Source:J Biol Chem;292(7):2679-2689, 2017 Feb 17.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The negative regulator of Rho family GTPases, p190A RhoGAP, is one of six mammalian proteins harboring so-called FF motifs. To explore the function of these and other p190A segments, we identified interacting proteins by tandem mass spectrometry. Here we report that endogenous human p190A, but not its 50% identical p190B paralog, associates with all 13 eIF3 subunits and several other translational preinitiation factors. The interaction involves the first FF motif of p190A and the winged helix/PCI domain of eIF3A, is enhanced by serum stimulation and reduced by phosphatase treatment. The p190A/eIF3A interaction is unaffected by mutating phosphorylated p190A-Tyr , but disrupted by a S296A mutation, targeting the only other known phosphorylated residue in the first FF domain. The p190A-eIF3 complex is distinct from eIF3 complexes containing S6K1 or mammalian target of rapamycin (mTOR), and appears to represent an incomplete preinitiation complex lacking several subunits. Based on these findings we propose that p190A may affect protein translation by controlling the assembly of functional preinitiation complexes. Whether such a role helps to explain why, unique among the large family of RhoGAPs, p190A exhibits a significantly increased mutation rate in cancer remains to be determined.
[Mh] Termos MeSH primário: Fator de Iniciação 3 em Eucariotos/metabolismo
Fatores de Troca do Nucleotídeo Guanina/metabolismo
Biossíntese de Proteínas
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromatografia de Afinidade
Fator de Iniciação 3 em Eucariotos/química
Fator de Iniciação 3 em Eucariotos/genética
Técnicas de Silenciamento de Genes
Fatores de Troca do Nucleotídeo Guanina/química
Fatores de Troca do Nucleotídeo Guanina/genética
Células HeLa
Seres Humanos
Camundongos
Mutação de Sentido Incorreto
Células NIH 3T3
Ligação Proteica
Proteínas Repressoras/química
Proteínas Repressoras/genética
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ARHGAP35 protein, human); 0 (EIF3A protein, human); 0 (Eukaryotic Initiation Factor-3); 0 (Guanine Nucleotide Exchange Factors); 0 (Repressor Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.769216



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