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[PMID]: 28467316 [Au] Autor: Chen R; Cai X; Ma K; Zhou Y; Wang Y; Jiang T [Ad] Endereço: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China. [Ti] Título: The fabrication of double-layered chitosan/gelatin/genipin nanosphere coating for sequential and controlled release of therapeutic proteins. [So] Source: Biofabrication;9(2):025028, 2017 Jun 01. [Is] ISSN: 1758-5090 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition and genipin crosslinking. The surface morphology, physicochemical and mechanical properties of the coatings are explored. Cytochrome C is used as a therapeutic model protein and is successfully loaded on the inner and outer layers of the coating. The protein release can be controlled by the loading position, genipin concentration and thickness of the outer layer. Furthermore, the cell response to the coatings was evaluated. Real-time polymerase chain reactions, immunofluorescence staining and extracellular matrix mineralization assay confirmed that the functions of the loaded growth factor are fully preserved after fabrication. Overall, the d-CSG/G nanosphere coating could be a promising growth factor delivery system to promote bone tissue regeneration. [Mh] Termos MeSH primário: Biomimética/métodos Quitosana/química Materiais Revestidos Biocompatíveis/química Citocromos c/uso terapêutico Gelatina/química Iridoides/química Nanosferas/química [Mh] Termos MeSH secundário: Animais Proteína Morfogenética Óssea 2/química Calcificação Fisiológica Bovinos Reagentes para Ligações Cruzadas/química Preparações de Ação Retardada Matriz Extracelular/metabolismo Imunofluorescência Células Mesenquimais Estromais/citologia Nanosferas/ultraestrutura Osteocalcina/metabolismo Ratos Reação em Cadeia da Polimerase em Tempo Real Proteínas Recombinantes/química Soluções Espectroscopia de Infravermelho com Transformada de Fourier Propriedades de Superfície Fator de Crescimento Transformador beta/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bone Morphogenetic Protein 2); 0 (Coated Materials, Biocompatible); 0 (Cross-Linking Reagents); 0 (Delayed-Action Preparations); 0 (Iridoids); 0 (Recombinant Proteins); 0 (Solutions); 0 (Transforming Growth Factor beta); 0 (recombinant human bone morphogenetic protein-2); 104982-03-8 (Osteocalcin); 9000-70-8 (Gelatin); 9007-43-6 (Cytochromes c); 9012-76-4 (Chitosan); A3V2NE52YG (genipin) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180309 [Lr] Data última revisão: 180309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170504 [St] Status: MEDLINE [do] DOI: 10.1088/1758-5090/aa70c3

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[PMID]: 28455252 [Au] Autor: Kwak HW; Shin M; Lee JY; Yun H; Song DW; Yang Y; Shin BS; Park YH; Lee KH [Ad] Endereço: Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. [Ti] Título: Fabrication of an ultrafine fish gelatin nanofibrous web from an aqueous solution by electrospinning. [So] Source: Int J Biol Macromol;102:1092-1103, 2017 Sep. [Is] ISSN: 1879-0003 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Electrospinning of aqueous gelatin solution obtained from bovine or porcine sources has been difficult to achieve without additional facilities, such as a temperature control oven or heating cover. Gelatin from cold-water fish has low contents of proline (Pro) and hydroxyproline (Hyp) compared with mammalian-derived gelatin. For this reason, the fish-derived gelatin maintains a sol state without showing gelation behavior at room temperature. In the present study, we prepared an ultrafine fish gelatin nanofibrous web by electrospinning from aqueous solutions without any additive polymers or temperature control facilities. The concentration and viscosity of fish gelatin are the most important factor in determining the electrospinnability and fiber diameter. Electrospinning of aqueous fish gelatin has the highest nanofiber productivity compared to other organic solvent systems. Using glutaraldehyde vapor (GTA), the water stability was improved and substantial enhancement was achieved in the mechanical properties. Finally, the cytotoxicity of a fish gelatin nanofibrous scaffold was evaluated based on a cell proliferation study by culturing human dermal fibroblasts (HDFs) compared with a fish gelatin film and nanofibrous mat from mammalian gelatin. The result shows better initial cell attachment and proliferation compared with the fish gelatin film and no significant difference compared with mammalian-derived gelatin nanofibrous mat. We expect that electrospinning of aqueous fish gelatin could be an effective alternative mammalian gelatin source. [Mh] Termos MeSH primário: Eletricidade Peixes Gelatina/química Nanofibras/química Nanotecnologia Água/química [Mh] Termos MeSH secundário: Animais Materiais Biocompatíveis/química Materiais Biocompatíveis/farmacologia Bovinos Adesão Celular/efeitos dos fármacos Proliferação Celular/efeitos dos fármacos Fibroblastos/citologia Fibroblastos/efeitos dos fármacos Gelatina/farmacologia Glutaral/química Seres Humanos Hidrólise Reologia Soluções Viscosidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biocompatible Materials); 0 (Solutions); 059QF0KO0R (Water); 9000-70-8 (Gelatin); T3C89M417N (Glutaral) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180309 [Lr] Data última revisão: 180309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170430 [St] Status: MEDLINE

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[PMID]: 29037702 [Au] Autor: Benbettaïeb N; Tanner C; Cayot P; Karbowiak T; Debeaufort F [Ad] Endereço: Univ. Bourgogne Franche-Comté/Agrosup Dijon, UMR PAM A02-102, Food and Wine Physical-Chemistry Lab, 1 esplanade Erasme, 21000 Dijon, France; IUT-Dijon-Auxerre, Dpt BioEngineering, 7 blvd Docteur Petitjean, 20178 Dijon Cedex, France. [Ti] Título: Impact of functional properties and release kinetics on antioxidant activity of biopolymer active films and coatings. [So] Source: Food Chem;242:369-377, 2018 Mar 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: This work deals with the study of the release kinetics of some natural antioxidants (ferulic acid, caffeic acid and tyrosol) from chitosan-fish gelatin edible films immersed ethanol at 96%, as well as the kinetics of their antioxidant activity using the DPPH assay. The aim was to determine how film functional properties influence the release kinetic and antioxidant activity. The addition of antioxidants to chitosan-fish gelatin matrix decreased the water vapour permeability by more than 30%. The tensile strength (TS) increased up to 50% after the incorporation of antioxidants. Some molecular interactions between polymer chains and antioxidants were confirmed by FTIR where spectra displayed a shift of the amide-III peak. Films containing caffeic acid or a caffeic-ferulic acid mixture exhibited the highest radical scavenging activity, leading to a 90% antioxidant activity at equilibrium but the release rate controlled the efficacy of the system. [Mh] Termos MeSH primário: Antioxidantes/análise Biopolímeros/química Quitosana/química Gelatina/química [Mh] Termos MeSH secundário: Antioxidantes/química Ácidos Cafeicos/análise Ácidos Cafeicos/química Ácidos Cumáricos/análise Ácidos Cumáricos/química Produtos Pesqueiros Cinética Permeabilidade Álcool Feniletílico/análogos & derivados Álcool Feniletílico/análise Álcool Feniletílico/química Resistência à Tração [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Biopolymers); 0 (Caffeic Acids); 0 (Coumaric Acids); 1AK4MU3SNX (4-hydroxyphenylethanol); 9000-70-8 (Gelatin); 9012-76-4 (Chitosan); AVM951ZWST (ferulic acid); ML9LGA7468 (Phenylethyl Alcohol); U2S3A33KVM (caffeic acid) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171018 [St] Status: MEDLINE

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[PMID]: 29251503 [Au] Autor: Ge S; Li M; Ji N; Liu J; Mul H; Xiong L; Sun Q [Ad] Endereço: College of Food Science and Engineering and ‡Central Laboratory, Qingdao Agricultural University Qingdao, Shandong Province 266109, China. [Ti] Título: Preparation of a Strong Gelatin-Short Linear Glucan Nanocomposite Hydrogel by an in Situ Self-Assembly Process. [So] Source: J Agric Food Chem;66(1):177-186, 2018 Jan 10. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Gelatin hydrogels exhibit excellent biocompatibility, nonimmunogenicity, and biodegradability, but they have limited applications in the food and medical industries because of their poor mechanical properties. Herein, we first developed an in situ self-assembly process for the preparation of gelatin-short linear glucan (SLG) nanocomposite hydrogels with enhanced mechanical strength. The microstructure, dynamic viscoelasticity, compression behavior, and thermal characteristics of the gelatin-SLG nanocomposite hydrogels were determined using scanning electron microscopy (SEM), dynamic rheological experiments, compression tests, and texture profile analysis tests. The SEM images revealed that nanoparticles were formed by the in situ self-assembly of SLG in the gelatin matrix and that the size of these nanoparticles ranged between 200 and 600 nm. The pores of the nanocomposite hydrogels were smaller than those of the pure gelatin hydrogels. Transmission electron microscopy images and X-ray diffraction further confirmed the presence of SLG nanoparticles with spherical shapes and B-type structures. Compared with pure gelatin hydrogels, the nanocomposite hydrogels exhibited improved mechanical behavior. Notably, the hardness and maximum values of the compressive stress of gelatin-SLG nanocomposites containing 5% SLG increased by about 2-fold and 3-fold, respectively, compared to the corresponding values of pure gelatin hydrogels. [Mh] Termos MeSH primário: Gelatina/química Glucanos/química Hidrogéis/química Nanocompostos/química [Mh] Termos MeSH secundário: Varredura Diferencial de Calorimetria Difusão Dinâmica da Luz Dureza Microscopia Eletrônica de Varredura Microscopia Eletrônica de Transmissão Nanocompostos/ultraestrutura Nanopartículas/química Reologia Espectroscopia de Infravermelho com Transformada de Fourier Viscosidade Difração de Raios X [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Glucans); 0 (Hydrogels); 9000-70-8 (Gelatin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171219 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b04684

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[PMID]: 28456988 [Au] Autor: Hashimoto Y [Ad] Endereço: Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, Kusumoto-cho, Chikusa-ku, Nagoya, 464-8650, Japan. yokuteku@dpc.agu.ac.jp. [Ti] Título: Gelatin Zymography Using Leupeptin for the Detection of Various Cathepsin L Forms. [So] Source: Methods Mol Biol;1594:243-254, 2017. [Is] ISSN: 1940-6029 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Zymography is a highly sensitive method to assess the activities as well as molecular weights of enzymes in crude biological fluids and tissue extracts. Cathepsin L is a lysosomal cysteine proteinase that is optimally active at slightly acidic pH and is highly unstable in alkaline solutions such as electrode buffer (pH 8.3). Large amounts of cathepsin L are secreted by various cancer cells, where it promotes invasion and metastasis. Leupeptin is a tight-binding inhibitor of cysteine proteinases, and its complex with cathepsin L is stable in alkaline solutions. Moreover, leupeptin can be easily removed from the complex because it is a reversibly binding inhibitor. In addition, leupeptin is too small to influence the electrode migration distance of the complex with cathepsin L on a sodium dodecyl sulfate-polyacrylamide gel. Here, a novel gelatin zymography technique that employs leupeptin to detect pro-, intermediate, and mature cathepsin L forms on the basis of their gelatinolytic activities is described. Further, the differences in the glycosylation, phosphorylation, and processing statuses of lysosomal and secreted cathepsin L forms isolated from cultured HT 1080 cells are demonstrated using this method. [Mh] Termos MeSH primário: Catepsina L/análise Gelatina/análise [Mh] Termos MeSH secundário: Catepsina L/química Células Cultivadas Ensaios Enzimáticos Gelatina/química Seres Humanos Leupeptinas/análise Leupeptinas/química Lisossomos/metabolismo Fosforilação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Leupeptins); 9000-70-8 (Gelatin); EC 3.4.22.15 (Cathepsin L); J97339NR3V (leupeptin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170501 [St] Status: MEDLINE [do] DOI: 10.1007/978-1-4939-6934-0_16

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[PMID]: 29272917 [Au] Autor: Liping S; Qiuming L; Jian F; Xiao L; Yongliang Z [Ad] Endereço: Yunnan Institute of Food Safety, Kunming University of Science and Technology , 727 South Jingming Road, Kunming, Yunnan 650500, People's Republic of China. [Ti] Título: Purification and Characterization of Peptides Inhibiting MMP-1 Activity with C Terminate of Gly-Leu from Simulated Gastrointestinal Digestion Hydrolysates of Tilapia (Oreochromis niloticus) Skin Gelatin. [So] Source: J Agric Food Chem;66(3):593-601, 2018 Jan 24. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Tilapia skin gelatin hydrolysates (TSGHs) were prepared by simulated gastrointestinal digestion and separated by gel filtration and semi-preparative reversed-phase high-performance liquid chromatography. The anti-photoaging effects were evaluated using an ultraviolet radiation B (UVB)-induced mouse embryonic fibroblast (MEF) photoaging model in vitro. Three fractions from TSGHs with high inhibitory intercellular matrix metalloproteinase-1 (MMP-1) activities and reactive oxygen species (ROS) production were obtained. Three key peptides, GYTGL, LGATGL, and VLGL, were identified, and their C terminate was Gly-Leu. Three peptides were synthesized and exhibited a significant inhibition of intercellular MMP-1 activity and ROS production. Furthermore, three peptides inhibiting MMP-1 activities were evaluated through their docking of S ' and S ' active pockets of MMP-1. Hydrogen bonds and C terminate Gly-Leu played important roles. Finally, the protective effects of three peptides on intercellular collagen in UVB-induced MEFs were compared. Our results indicated that tilapia gelatin peptides exhibited potential activities to prevent and regulate photoaging. [Mh] Termos MeSH primário: Gelatina/química Inibidores de Metaloproteinases de Matriz/química Peptídeos/química Pele/química [Mh] Termos MeSH secundário: Motivos de Aminoácidos Animais Antioxidantes/química Antioxidantes/isolamento & purificação Antioxidantes/farmacologia Colágeno/metabolismo Digestão Fibroblastos/efeitos dos fármacos Fibroblastos/enzimologia Fibroblastos/metabolismo Fibroblastos/efeitos da radiação Proteínas de Peixes/química Proteínas de Peixes/isolamento & purificação Proteínas de Peixes/farmacologia Trato Gastrointestinal/metabolismo Gelatina/isolamento & purificação Gelatina/farmacologia Metaloproteinase 1 da Matriz/química Metaloproteinase 1 da Matriz/metabolismo Inibidores de Metaloproteinases de Matriz/isolamento & purificação Camundongos Modelos Biológicos Simulação de Acoplamento Molecular Peptídeos/isolamento & purificação Peptídeos/farmacologia Espécies Reativas de Oxigênio/metabolismo Pele/metabolismo Tilápia Raios Ultravioleta [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Fish Proteins); 0 (Matrix Metalloproteinase Inhibitors); 0 (Peptides); 0 (Reactive Oxygen Species); 9000-70-8 (Gelatin); 9007-34-5 (Collagen); EC 3.4.24.7 (Matrix Metalloproteinase 1) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180207 [Lr] Data última revisão: 180207 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171224 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b04196

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[PMID]: 29025650 [Au] Autor: Lefebvre E; Lembre P; Picard J; El-Guermah L; Seyer D; Larreta Garde V [Ad] Endereço: ERRMECe Laboratory, University of Cergy-Pontoise, France; Biology Department/ERRMECe, University of Cergy-Pontoise, France. [Ti] Título: Ephemeral biogels to control anti-biofilm agent delivery: From conception to the construction of an active dressing. [So] Source: Mater Sci Eng C Mater Biol Appl;82:210-216, 2018 Jan 01. [Is] ISSN: 1873-0191 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Chronic wound colonization by bacterial biofilms is common and can cause various complications. An anti-biofilm strategy was developed around the co-entrapment of a commercially available antiseptic, PHMB (polyhexamethylene biguanide 4mgmL ), with EDTA (Ethylen diamine tetra acetic acid, 20mM) in a gelatin gel. The two active compounds act synergistically against bacterial biofilms, but their efficiency is strongly reduced (16-fold) when entrapped inside the 5% gelatin gel, and they weaken the mechanical properties (50-fold) of the gel. Increasing the gelatin concentration to 7% allows for good mechanical properties but large diffusional constraints. An active ephemeral gel, a chemical gel with controlled hydrolysis, was conceived and developed. When the ephemeral gel was solubilized after 48h, PHMB delivery increased, leading to good anti-biofilm activity. The various gels were examined over 24 and 48h of contact with P. aeruginosa and S. aureus biofilms, two types of bacterial biofilms frequently encountered in chronic wounds. The ephemeral gel eradicated the dense biofilms (>6.10 CFU·cm ) produced by either single or mixed strains; a similar efficiency was measured for biofilms from strains of both laboratory and clinical origin. The formulation was then adapted to develop a dressing prototype that is active against biofilms and fulfils the requirements of an efficient wound care system. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Biguanidas/química Biofilmes/efeitos dos fármacos Ácido Edético/química Géis/química [Mh] Termos MeSH secundário: Antibacterianos/síntese química Antibacterianos/química Bandagens Biguanidas/farmacologia Ácido Edético/farmacologia Gelatina/química Pseudomonas aeruginosa/fisiologia Reologia Staphylococcus aureus/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Biguanides); 0 (Gels); 322U039GMF (polihexanide); 9000-70-8 (Gelatin); 9G34HU7RV0 (Edetic Acid) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171014 [St] Status: MEDLINE

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[PMID]: 29025651 [Au] Autor: Feng S; He F; Ye J [Ad] Endereço: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China. [Ti] Título: Hierarchically porous structure, mechanical strength and cell biological behaviors of calcium phosphate composite scaffolds prepared by combination of extrusion and porogen burnout technique and enhanced by gelatin. [So] Source: Mater Sci Eng C Mater Biol Appl;82:217-224, 2018 Jan 01. [Is] ISSN: 1873-0191 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: In this study, hierarchically porous calcium phosphate scaffolds (HTCP) with unidirectional pores, transversely interconnected pores, and micropores were fabricated by the combination of extrusion and porogen burnout technique. Gelatin was incorporated into the HTCP scaffolds by vacuum-impregnation of gelatin solution and subsequent freeze-drying. The phase composition, microstructure, physical and cytobiological properties were analyzed. The results showed that the HTCP scaffolds were composed of ß-tricalcium phosphate with minor hydroxyapatite. The HTCP scaffolds had unidirectional pores (~400µm), transversely interconnected pores (~130µm) and micropores (~1µm). The incorporation of gelatin significantly increased the compressive strength, toughness, and cell seeding of the HTCP scaffolds. The composite scaffolds showed excellent cytocompatibility. The hierarchically porous calcium phosphate composite scaffolds may have potential application prospects in bone tissue engineering. [Mh] Termos MeSH primário: Materiais Biocompatíveis/química Fosfatos de Cálcio/química Gelatina/química Engenharia Tecidual/métodos Tecidos Suporte/química [Mh] Termos MeSH secundário: Animais Materiais Biocompatíveis/farmacologia Células da Medula Óssea/citologia Adesão Celular/efeitos dos fármacos Proliferação Celular/efeitos dos fármacos Células Cultivadas Força Compressiva Liofilização Células Mesenquimais Estromais/citologia Células Mesenquimais Estromais/metabolismo Camundongos Porosidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biocompatible Materials); 0 (Calcium Phosphates); 9000-70-8 (Gelatin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171014 [St] Status: MEDLINE

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[PMID]: 28832260 [Au] Autor: Davoudi Z; Rabiee M; Houshmand B; Eslahi N; Khoshroo K; Rasoulianboroujeni M; Tahriri M; Tayebi L [Ad] Endereço: a Biomaterials Group, Faculty of Biomedical Engineering , Amirkabir University of Technology , Tehran , Iran. [Ti] Título: Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis. [So] Source: Drug Dev Ind Pharm;44(1):40-55, 2018 Jan. [Is] ISSN: 1520-5762 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process. Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region. In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin. In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films. Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug. The results of surface pH showed that none of the samples were harmful to the buccal cavity. FTIR analysis confirmed the influence of keratin on the structure of the composite. The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 10 U/ml lysozyme. The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate. Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications. [Mh] Termos MeSH primário: Quitosana/química Gelatina/química Gengivite/tratamento farmacológico Hidrocortisona/análogos & derivados Hidrocortisona/química Queratinas/química Succinatos/química [Mh] Termos MeSH secundário: Adesividade Hidrocortisona/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Succinates); 68238-35-7 (Keratins); 9000-70-8 (Gelatin); 9012-76-4 (Chitosan); LIU00Z1Z84 (cortisol succinate); WI4X0X7BPJ (Hydrocortisone) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180111 [Lr] Data última revisão: 180111 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170824 [St] Status: MEDLINE [do] DOI: 10.1080/03639045.2017.1371738

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[PMID]: 28456652 [Au] Autor: Schwabe K; Ewe A; Kohn C; Loth T; Aigner A; Hacker MC; Schulz-Siegmund M [Ad] Endereço: Leipzig University, Institute of Pharmacy, Pharmaceutical Technology, Germany. [Ti] Título: Sustained delivery of siRNA poly- and lipopolyplexes from porous macromer-crosslinked gelatin gels. [So] Source: Int J Pharm;526(1-2):178-187, 2017 Jun 30. [Is] ISSN: 1873-3476 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: RNA interference (RNAi) is a promising technique to treat severe diseases on a pre-protein level. We and others postulate that the release of nanoparticle-complexed small interfering RNA (siRNA) from implanted biomaterials could provide structural support for tissue repair, combined with local siRNA transfection of invading and regenerating cells. In this study, we systematically investigated cross-linked gelatin based hydrogel formulations (cGEL) as degradable controlled release matrices for siRNA. Aiming at the definition of correlations between cGEL composition, siRNA nanoparticle formulation, release kinetics of complexed siRNA and transfection efficiency, we combined five different cGEL formulations and three transfection systems, i.e. polyplexes with polyethyleneimine (PEI), PEI in combination with liposomes (lipopolyplexes) and polyplexes based on tyrosin-modified PEI (P10Y). It was found that the distribution of these poly-/lipopolyplexes, when applied onto the negatively charged hydrogels, was strongly dependent on their zeta potential. Furthermore, siRNA release from the hydrogel was a multifactorial process, as diffusion, hydrogel degradation and nanoparticle decomplexation overlapped over time. This resulted in a prolonged release of siRNA for up to 21days. In the case of PEI complexes and lipopolyplexes, release kinetics depended on the cGEL formulation. In contrast, when employing P10Y polyplexes, an initial burst release was observed with no further release thereafter. Silencing activity was determined using constitutively luciferase-expressing SKOV-3-Luc reporter cells. Surface and bulk porosity in hydrogels was introduced by addition of soluble polyethylene glycol during fabrication, leading to improved knockdown. The rapid onset of knockdown efficacy will also provide the basis for the determination of long-term effects. [Mh] Termos MeSH primário: Gelatina/química Hidrogéis/química RNA Interferente Pequeno/administração & dosagem Transfecção/métodos [Mh] Termos MeSH secundário: Linhagem Celular Tumoral Seres Humanos Polietilenoimina Interferência de RNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Hydrogels); 0 (RNA, Small Interfering); 9000-70-8 (Gelatin); 9002-98-6 (Polyethyleneimine) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180104 [Lr] Data última revisão: 180104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170501 [St] Status: MEDLINE

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		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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