Base de dados : MEDLINE
Pesquisa : D12.776.866 [Categoria DeCS]
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[PMID]:28450256
[Au] Autor:Dietrich MA; Irnazarow I; Ciereszko A
[Ad] Endereço:Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. Electronic address: m.dietrich@pan.olsztyn.pl.
[Ti] Título:Proteomic identification of seminal plasma proteins related to the freezability of carp semen.
[So] Source:J Proteomics;162:52-61, 2017 Jun 06.
[Is] ISSN:1876-7737
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The variation in sperm freezability among individuals within a fish species is a major factor justifying the identification of useful predictive indicators of cryopreservation success. It is unknown at present whether the protein composition of fish seminal plasma affects sperm freezability. Therefore, the aims of this study were to compare the proteome of carp seminal plasma from semen rated as good (GF) and poor (PF) freezability by two-dimensional difference gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF and PF based on sperm motility assessment after freeze/thawing. Five spots representing three proteins were more abundant in GF, while ten spots representing seven proteins were more abundant in PF seminal plasma. The majority of proteins present in higher abundance in PF seminal plasma were associated with the innate immune response. On the other hand, higher freezability was associated with proteins involved in the maintenance of sperm membrane integrity and antioxidative protection. These results indicate that carp semen freezability levels may be related to different seminal plasma protein profiles. Lower usefulness of spermatozoa in cryopreservation may be related to previous infection or stress leading to sublethal changes to sperm structure. SIGNIFICANCE: Sperm quality parameters such as motility, viability and sperm concentration have been used as predictive tools of sperm cryopreservation potential in fish species However, the usefulness of initial motility parameters as indicators of freezability varies among fish species and between individuals within a species. Recent studies in mammals revealed that male-to-male variability in cryoresistance can be attributed to differences in seminal plasma protein composition. To the best of our knowledge, no proteomic studies linking the protein composition of fish seminal plasma and freezing resilience have been performed in fish. Our results indicate for the first time that factors regulating how carp semen tolerate cryopreservation may be related to the different protein profiles of carp seminal plasma. The obtained results provide new insight into understanding the molecular mechanisms underlying cryoresistance of carp semen and provide a tool for the improvement of a long-term sperm preservation procedure.
[Mh] Termos MeSH primário: Carpas
Congelamento
Sêmen/química
Proteínas de Plasma Seminal/análise
[Mh] Termos MeSH secundário: Animais
Criopreservação
Masculino
Proteômica/métodos
Sêmen/citologia
Espermatozoides/citologia
Espectrometria de Massas em Tandem
Eletroforese em Gel Diferencial Bidimensional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Seminal Plasma Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:28911169
[Au] Autor:Borges BC; Garcia-Galiano D; da Silveira Cruz-Machado S; Han X; Gavrilina GB; Saunders TL; Auchus RJ; Hammoud SS; Smith GD; Elias CF
[Ad] Endereço:Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan 48109.
[Ti] Título:Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity.
[So] Source:Endocrinology;158(9):2930-2943, 2017 Sep 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Approximately 15% of human couples of reproductive age have impaired fertility, and the male component accounts for about half of these cases. The etiology is usually unknown, but high correlation with the increase in obesity rates is documented. In this study, we show that diet-induced and genetically obese mice display copulatory behavior comparable to controls, but the number of females impregnated by obese males is remarkably low. Screening for changes in gene expression in the male reproductive tract showed decreased Crisp4 expression in testis and epididymis of obese mice. Lack of CRISP4 in the luminal membrane of epididymal cells indicated inadequate secretion. Consistent with CRISP4 action in acrosome reaction, sperm from mice fed a high-fat diet (HFD) had decreased fertilization capacity. CRISP4 treatment of sperm from HFD mice prior to in vitro fertilization improved fertilization rate. In leptin-deficient obese and infertile mice, leptin's effect to restore CRISP4 expression and function required gonadal hormones. Our findings indicate that the obesity-induced decline in sperm motility and fertilization capacity results in part from the disruption of epididymal CRISP4 expression and secretion.
[Mh] Termos MeSH primário: Fertilização/genética
Infertilidade Masculina/etiologia
Obesidade/complicações
Proteínas de Plasma Seminal/genética
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Reação Acrossômica/genética
Animais
Epididimo/metabolismo
Feminino
Infertilidade Masculina/genética
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Obesos
Camundongos Transgênicos
Obesidade/genética
Proteínas de Plasma Seminal/metabolismo
Motilidade Espermática/genética
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Seminal Plasma Proteins); 0 (cysteine-rich secretory protein 4, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00295


  3 / 1372 MEDLINE  
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[PMID]:28663133
[Au] Autor:Marchak A; Grant PA; Neilson KM; Datta Majumdar H; Yaklichkin S; Johnson D; Moody SA
[Ad] Endereço:Department of Anatomy and Regenerative Biology, George Washington University School of Medicine and Health Sciences, Washington DC, USA.
[Ti] Título:Wbp2nl has a developmental role in establishing neural and non-neural ectodermal fates.
[So] Source:Dev Biol;429(1):213-224, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In many animals, maternally synthesized mRNAs are critical for primary germ layer formation. In Xenopus, several maternal mRNAs are enriched in the animal blastomere progenitors of the embryonic ectoderm. We previously identified one of these, WW-domain binding protein 2 N-terminal like (wbp2nl), that others previously characterized as a sperm protein (PAWP) that promotes meiotic resumption. Herein we demonstrate that it has an additional developmental role in regionalizing the embryonic ectoderm. Knock-down of Wbp2nl in the dorsal ectoderm reduced cranial placode and neural crest gene expression domains and expanded neural plate domains; knock-down in ventral ectoderm reduced epidermal gene expression. Conversely, increasing levels of Wbp2nl in the neural plate induced ectopic epidermal and neural crest gene expression and repressed many neural plate and cranial placode genes. The effects in the neural plate appear to be mediated, at least in part, by down-regulating chd, a BMP antagonist. Because the cellular function of Wbp2nl is not known, we mutated several predicted motifs. Expressing mutated proteins in embryos showed that a putative phosphorylation site at Thr45 and an α-helix in the PH-G domain are required to ectopically induce epidermal and neural crest genes in the neural plate. An intact YAP-binding motif also is required for ectopic epidermal gene expression as well as for down-regulating chd. This work reveals novel developmental roles for a cytoplasmic protein that promotes epidermal and neural crest formation at the expense of neural ectoderm.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Ectoderma/embriologia
Ectoderma/metabolismo
Sistema Nervoso/embriologia
Sistema Nervoso/metabolismo
Proteínas de Plasma Seminal/metabolismo
Proteínas de Xenopus/metabolismo
Xenopus laevis/embriologia
Xenopus laevis/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas Morfogenéticas Ósseas/metabolismo
Proteínas de Transporte/química
Proteínas de Transporte/genética
Epiderme/embriologia
Epiderme/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Mesoderma/embriologia
Mesoderma/metabolismo
Mutação/genética
Crista Neural/embriologia
Crista Neural/metabolismo
Placa Neural/embriologia
Placa Neural/metabolismo
Fenótipo
Domínios Proteicos
Transporte Proteico
Proteínas de Plasma Seminal/química
Proteínas de Plasma Seminal/genética
Alinhamento de Sequência
Proteínas de Xenopus/química
Proteínas de Xenopus/genética
Xenopus laevis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bone Morphogenetic Proteins); 0 (Carrier Proteins); 0 (Seminal Plasma Proteins); 0 (Wbp2nl protein, Xenopus); 0 (Xenopus Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE


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[PMID]:28493870
[Au] Autor:Sharma V; Pandey AK; Kumar A; Misra S; Gupta HPK; Gupta S; Singh A; Buehner NA; Ravi Ram K
[Ad] Endereço:Embryotoxicology Laboratory, Environmental Toxicology Group, CSIR- Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhavan, 31, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, India.
[Ti] Título:Functional male accessory glands and fertility in Drosophila require novel ecdysone receptor.
[So] Source:PLoS Genet;13(5):e1006788, 2017 May.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In many insects, the accessory gland, a secretory tissue of the male reproductive system, is essential for male fertility. Male accessory gland is the major source of proteinaceous secretions, collectively called as seminal proteins (or accessory gland proteins), which upon transfer, manipulate the physiology and behavior of mated females. Insect hormones such as ecdysteroids and juvenoids play a key role in accessory gland development and protein synthesis but little is known about underlying molecular players and their mechanism of action. Therefore, in the present study, we examined the roles of hormone-dependent transcription factors (Nuclear Receptors), in accessory gland development, function and male fertility of a genetically tractable insect model, Drosophila melanogaster. First, we carried out an RNAi screen involving 19 hormone receptors, individually and specifically, in a male reproductive tissue (accessory gland) for their requirement in Drosophila male fertility. Subsequently, by using independent RNAi/ dominant negative forms, we show that Ecdysone Receptor (EcR) is essential for male fertility due to its requirement in the normal development of accessory glands in Drosophila: EcR depleted glands fail to make seminal proteins and have dying cells. Further, our data point to a novel ecdysone receptor that does not include Ultraspiracle but is probably comprised of EcR isoforms in Drosophila male accessory glands. Our data suggest that this novel ecdysone receptor might act downstream of homeodomain transcription factor paired (prd) in the male accessory gland. Overall, the study suggests novel ecdysone receptor as an important player in the hormonal regulation of seminal protein production and insect male fertility.
[Mh] Termos MeSH primário: Proteínas de Drosophila/genética
Ecdisteroides/genética
Proteínas de Homeodomínio/genética
Infertilidade Masculina/genética
Receptores Citoplasmáticos e Nucleares/genética
Receptores de Esteroides/genética
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Drosophila melanogaster/genética
Drosophila melanogaster/crescimento & desenvolvimento
Ecdisteroides/metabolismo
Feminino
Fertilidade/genética
Masculino
Receptores Citoplasmáticos e Nucleares/metabolismo
Reprodução/genética
Proteínas de Plasma Seminal/genética
Proteínas de Plasma Seminal/metabolismo
Espermatozoides/crescimento & desenvolvimento
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Ecdysteroids); 0 (Homeodomain Proteins); 0 (Receptors, Cytoplasmic and Nuclear); 0 (Receptors, Steroid); 0 (Seminal Plasma Proteins); 0 (ecdysone receptor); 0 (paired protein, Drosophila)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006788


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[PMID]:28419103
[Au] Autor:Aass C; Norheim I; Eriksen EF; Børnick EC; Thorsby PM; Pepaj M
[Ad] Endereço:Hormone Laboratory, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
[Ti] Título:Establishment of a tear protein biomarker panel differentiating between Graves' disease with or without orbitopathy.
[So] Source:PLoS One;12(4):e0175274, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Graves' orbitopathy (GO) is an autoimmune inflammatory ocular complication and one of the most frequent manifestations of Graves' disease (GD). Clinical judgment of GO is subjective sometimes leading to clinical and therapeutic challenges. Better tools to diagnose this severe complication are warranted. PATIENTS AND METHODS: The aim of the present study was to evaluate tear levels of LYZ, LACRT and AZGP1 in GD patients with or without GO, as possible biomarkers for GO. Tear samples were collected from GD patients with moderate-to-severe GO (n = 21) and no clinical signs of GO (n = 21). Additionally, 18 GD patients with mild GO and 9 patients without GO were included in a further part of the study. RESULTS: Tear levels of LYZ (p < 0.001), LACRT (p = 0.004) and AZGP1 (p = 0.001) were significantly elevated in GD patients with moderate-to-severe GO compared to GD patients without GO. The discriminatory power of the three biomarkers, combined in a panel was confirmed by ROC plot analysis, with an AUC value of 0.93 (sensitivity of 95%; specificity of 80%). Since LYZ showed the best performance in discriminating between GD patients with (moderate-to-severe) and without GO (in combination with limited sample volume available), LYZ levels were also measured in tears from GD patients with mild GO and without GO. Significantly higher levels of LYZ were measured in GD patients with mild GO compared to those without GO (p = 0.003). CONCLUSIONS: We have established a novel three-protein biomarker panel that is able to discriminate between GD patients with and without GO, which might aid in diagnostic evaluation of GO as well as an indicator for disease activity.
[Mh] Termos MeSH primário: Biomarcadores/análise
Proteínas do Olho/análise
Doença de Graves/metabolismo
Oftalmopatia de Graves/metabolismo
Lágrimas/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Diagnóstico Diferencial
Ensaio de Imunoadsorção Enzimática
Feminino
Glicoproteínas/análise
Doença de Graves/diagnóstico
Oftalmopatia de Graves/diagnóstico
Seres Humanos
Masculino
Meia-Idade
Muramidase/análise
Curva ROC
Proteínas de Plasma Seminal/análise
Índice de Gravidade de Doença
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Eye Proteins); 0 (Glycoproteins); 0 (LACRT protein, human); 0 (Seminal Plasma Proteins); 0 (Zn-alpha-2-glycoprotein); 0 (tear proteins); EC 3.2.1.17 (Muramidase); EC 3.2.1.17 (lysozyme C, human)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175274


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[PMID]:28364784
[Au] Autor:Shaveisi-Zadeh F; Alibakhshi R; Asgari R; Rostami-Far Z; Bakhtiari M; Abdi H; Movafagh A; Mirfakhraie R
[Ad] Endereço:Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:TTY2 genes deletions as genetic risk factor of male infertility.
[So] Source:Cell Mol Biol (Noisy-le-grand);63(2):57-61, 2017 Feb 28.
[Is] ISSN:1165-158X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Y chromosome has a number of genes that are expressed in testis and have a role in spermatogenesis. TTY2L12A and TTY2L2A are the members of testis transcript Y2 (TTY2) that are Y linked multi-copy gene families, located on Yp11 and Yq11 loci respectively. The aim of this study was to investigate frequency of TTY2L12A and TTY2L2A deletions in azoospermic patients compared with fertile males. This study was performed on 45 infertile males with idiopathic azoospermia without any AZF micro deletions (group A), 33 infertile males with azoospermia which do not screened for AZF micro deletions (group B) and 65 fertile males (group C), from October 2013 to April 2015 in west of Iran. Polymerase chain reaction (PCR) method was used for detection of TTY2L12A and TTY2L2A gene deletions in studied groups. No deletions were detected in normal fertile males of group C. 1 out of 45 azoospermic males of group A (2.22%) and 3 out of 33 azoospermic males of group B (9.09%) had TTY2L2A deletion (p= 0.409 and p= 0.036 respectively), also 1 out of 45 azoospermic males of group A (2.22%) and 4 out of 33 azoospermic males of group B (12.12%) had TTY2L12A deletion (p= 0.409 and p= 0.011 respectively).  None of azoospermic males in Group A and B had deletions in both genes. Our data showed significant correlation between non-obstructive azoospermia and TTY2L12A and TTY2L2A deletions. Thus, it seems that TTY2L12A and TTY2L2A deletions can consider as one of the genetic risk factors for non-obstructive azoospermia.
[Mh] Termos MeSH primário: Deleção de Genes
Predisposição Genética para Doença/genética
Infertilidade Masculina/genética
Proteínas de Plasma Seminal/genética
[Mh] Termos MeSH secundário: Adulto
Azoospermia/genética
Estudos de Casos e Controles
Cromossomos Humanos Y/genética
Frequência do Gene
Seres Humanos
Irã (Geográfico)
Masculino
Reação em Cadeia da Polimerase
Fatores de Risco
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Seminal Plasma Proteins); 0 (TTTY2 protein, human)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170426
[Lr] Data última revisão:
170426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE
[do] DOI:10.14715/cmb/2017.63.2.8


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[PMID]:28284716
[Au] Autor:Na HS; Kwon JE; Lee SH; Jhun J; Kim SM; Kim SY; Kim EK; Jung K; Park SH; Cho ML
[Ad] Endereço:Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
[Ti] Título:Th17 and IL-17 Cause Acceleration of Inflammation and Fat Loss by Inducing α -Glycoprotein 1 (AZGP1) in Rheumatoid Arthritis with High-Fat Diet.
[So] Source:Am J Pathol;187(5):1049-1058, 2017 May.
[Is] ISSN:1525-2191
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rheumatoid arthritis (RA) is a chronic autoimmune disorder that affects the joints. High-fat diet (HFD) is a risk factor for RA and is related to inflammation but responds minimally to medication. Given the association between HFD and inflammation, it is important to understand the function of inflammation-related T cells in RA with HFD. Collagen-induced arthritis (CIA), a model of RA, was induced in HFD mice by injection of collagen II, and metabolic markers and T cells were analyzed. The metabolic index and IgG assay results were higher in HFD-CIA mice than in nonfat diet-CIA mice. Numbers of inflammation-related T cells and macrophages, such as Th1 and Th17 cells and M1 macrophages, were higher in spleens of HFD-CIA mice. HFD-CIA mice had a high level of α -glycoprotein 1 (Azgp1), a soluble protein that stimulates lipolysis. To examine the association between Azgp1 and Th17 cells, the reciprocal effects of Azgp1 and IL-17 on Th17 differentiation and lipid metabolism were measured. Interestingly, Azgp1 increased the Th17 population of splenocytes. Taken together, our data suggest that the acceleration of fat loss caused by Azgp1 in RA with metabolic syndrome is related to the increase of IL-17. Mice injected with the Azgp1-overexpression vector exhibited more severe CIA compared with the mock vector-injected mice.
[Mh] Termos MeSH primário: Artrite Reumatoide/etiologia
Dieta Hiperlipídica/efeitos adversos
Interleucina-17/fisiologia
Células Th17/fisiologia
[Mh] Termos MeSH secundário: Animais
Artrite Experimental/induzido quimicamente
Diferenciação Celular/fisiologia
Colágeno Tipo II/toxicidade
Vetores Genéticos/administração & dosagem
Vetores Genéticos/farmacologia
Glicogênio/metabolismo
Imunoglobulinas/metabolismo
Interleucina-17/farmacologia
Metabolismo dos Lipídeos/fisiologia
Masculino
Doenças Metabólicas/fisiopatologia
Camundongos Endogâmicos DBA
Proteínas Recombinantes/farmacologia
Proteínas de Plasma Seminal/metabolismo
Baço/citologia
Linfócitos T Reguladores/fisiologia
Regulação para Cima/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type II); 0 (Immunoglobulins); 0 (Interleukin-17); 0 (Recombinant Proteins); 0 (Seminal Plasma Proteins); 0 (Zn-alpha-2-glycoprotein); 9005-79-2 (Glycogen)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  8 / 1372 MEDLINE  
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[PMID]:28086239
[Au] Autor:Mao Y; Xu R; Liu X; Shi W; Han Y
[Ad] Endereço:Department of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China.
[Ti] Título:Elevated fibrous sheath interacting protein 1 levels are associated with poor prognosis in non-small cell lung cancer patients.
[So] Source:Oncotarget;8(7):12186-12193, 2017 Feb 14.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we examined the expression and prognostic value of fibrous sheath interacting protein 1 (FSIP1) in 202 non-small cell lung cancer (NSCLC) patients who underwent lung cancer resection at Shengjing Hospital of China Medical University. FSIP1 mRNA and protein expression were measured in NSCLC tissues and non-tumor adjacent tissues (NATs), and Harrell's concordance index (c-index) was used to evaluate the ability of FSIP1 to predict prognosis. FSIP1 mRNA and protein expression was higher in NSCLC tissues than in NATs. Survival analysis revealed the 5-year overall survival rate to be 35.4% in the FSIP1-positive group and 56.3% in the FSIP1-negative group, and FSIP1-positive status was an independent prognostic factor for poor overall survival. The c-index value of FSIP1 for overall survival was greater than that of Ki67, and the addition of FSIP1 status increased the c-index value of the TNM staging system. These results suggest that evaluating FSIP1 status in addition to TNM stage during routine pathological examinations could improve prognostic predictions in NSCLC patients.
[Mh] Termos MeSH primário: Carcinoma Pulmonar de Células não Pequenas/genética
Proteínas de Transporte/genética
Regulação Neoplásica da Expressão Gênica
Predisposição Genética para Doença/genética
Neoplasias Pulmonares/genética
Proteínas de Plasma Seminal/genética
[Mh] Termos MeSH secundário: Grupo com Ancestrais do Continente Asiático/genética
Western Blotting
Carcinoma Pulmonar de Células não Pequenas/etnologia
Carcinoma Pulmonar de Células não Pequenas/metabolismo
Proteínas de Transporte/metabolismo
China
Feminino
Predisposição Genética para Doença/etnologia
Seres Humanos
Imuno-Histoquímica
Estimativa de Kaplan-Meier
Antígeno Ki-67/metabolismo
Neoplasias Pulmonares/etnologia
Neoplasias Pulmonares/metabolismo
Masculino
Meia-Idade
Análise Multivariada
Estadiamento de Neoplasias
Prognóstico
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas de Plasma Seminal/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (FSIP1 protein, human); 0 (Ki-67 Antigen); 0 (Seminal Plasma Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.14575


  9 / 1372 MEDLINE  
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[PMID]:28041731
[Au] Autor:Westfalewicz B; Dietrich MA; Mostek A; Partyka A; Bielas W; Nizanski W; Ciereszko A
[Ad] Endereço:Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. Electronic address: b.westfalewicz@pan.olsztyn.pl.
[Ti] Título:Analysis of bull (Bos taurus) seminal vesicle fluid proteome in relation to seminal plasma proteome.
[So] Source:J Dairy Sci;100(3):2282-2298, 2017 Mar.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The existing knowledge on the bull seminal vesicle proteome, a major seminal plasma constituent, and its relationship with seminal plasma is limited. This knowledge is prerequisite for a better understanding of seminal plasma variability, which is linked to semen quality. The objective of this study was to characterize the proteomes of seminal vesicle fluid and seminal plasma and to compare them to better understand the origin of seminal plasma proteins. We collected ejaculates and seminal vesicle fluid postmortem from 6 mature Holstein Friesian bulls. We performed the analysis and identification of proteins using 2-dimensional electrophoresis coupled with matrix-assisted laser desorption/ionization mass spectrometry. We identified 105 proteins in bull seminal vesicle fluid and 88 proteins in seminal plasma. For both seminal vesicles and seminal plasma proteins described in our study, top biological functions were cellular movement, cell death and survival, and cellular growth and proliferation. Additionally, seminal vesicle fluid proteins were involved in protein degradation and synthesis. Seminal plasma proteins were also involved in cellular assembly and organization and cell-to-cell signaling and interactions. Proteins of both fluids were involved in the following canonical pathways: glycolysis, gluconeogenesis, liver X receptor/farnesoid X receptor, and farnesoid X receptor/retinoid X receptor activation. Additionally, seminal vesicle fluid proteins appeared to be involved in oxidative stress response mediated by nuclear factor E2-related factor 2. Our results described the bull seminal vesicle fluid proteome for the first time and allowed for significant expansion of the current knowledge on the bull seminal plasma proteome. Moreover, analysis indicated that both bull seminal vesicle fluid and seminal plasma proteomes contained interconnected protein groups related to protective functions, glycolysis, and the morphology and physiology of the spermatozoa. These proteins and their interactions could be targeted in future research.
[Mh] Termos MeSH primário: Proteoma/metabolismo
Proteínas de Plasma Seminal
[Mh] Termos MeSH secundário: Animais
Bovinos
Masculino
Sêmen
Análise do Sêmen
Glândulas Seminais
Espermatozoides
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); 0 (Seminal Plasma Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170103
[St] Status:MEDLINE


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[PMID]:28013009
[Au] Autor:Kumar CS; Swamy MJ
[Ad] Endereço:School of Chemistry, University of Hyderabad, Hyderabad 500046, India.
[Ti] Título:Differential modulation of the chaperone-like activity of HSP-1/2, a major protein of horse seminal plasma by anionic and cationic surfactants.
[So] Source:Int J Biol Macromol;96:524-531, 2017 Mar.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The major protein of equine seminal plasma, HSP-1/2 exhibits chaperone-like activity (CLA) by protecting various target proteins against thermal, chemical and oxidative stress. Polydispersity and surface hydrophobicity of HSP-1/2 were found to be important for its CLA. Surfactants are known to alter certain properties of proteins, e.g. hydrophobicity, charge and conformation either by altering properties of the medium or by direct binding. In the current study, thermal aggregation of alcohol dehydrogenase (ADH) and enolase has been studied in the presence of HSP-1/2, different surfactants and their combinations. The results obtained show that anionic surfactants (SDS, sodium dodecyl benzene sulfate) and neutral surfactants (tween-20, triton X-100) increase the CLA of HSP-1/2 and also inhibit aggregation of the target proteins independently. On the other hand, cationic surfactants (CTAB, alanine palmityl ester) increased the thermal aggregation of ADH and enolase and also decreased the CLA of HSP-1/2. These results are of significant interest as they show that surfactants such as SDS and tween-20 can potentially be used as anti-aggregation agents to prevent thermal aggregation of target proteins.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Glicoproteínas/metabolismo
Cavalos
Sêmen/química
Proteínas de Plasma Seminal/metabolismo
Tensoativos/química
Tensoativos/farmacologia
[Mh] Termos MeSH secundário: Álcool Desidrogenase/química
Animais
Proteínas de Transporte/química
Glicoproteínas/química
Interações Hidrofóbicas e Hidrofílicas
Fosfopiruvato Hidratase/química
Agregados Proteicos
Estrutura Secundária de Proteína/efeitos dos fármacos
Proteínas de Plasma Seminal/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Glycoproteins); 0 (HSP-1 protein, Equus caballus); 0 (HSP-2 protein, Equus caballus); 0 (Protein Aggregates); 0 (Seminal Plasma Proteins); 0 (Surface-Active Agents); EC 1.1.1.1 (Alcohol Dehydrogenase); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE



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