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[PMID]: 28891315 [Au] Autor: Kammari K; Devaraya K; Bommakanti A; Kondapi AK [Ad] Endereço: Department of Biotechnology & Bioinformatics, School of Life Sciences, University of Hyderabad. [Ti] Título: Development of pyridine dicoumarols as potent anti HIV-1 leads, targeting HIV-1 associated topoisomeraseIIß kinase. [So] Source: Future Med Chem;9(14):1597-1609, 2017 Sep. [Is] ISSN: 1756-8927 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: AIM: A structural study of a series of pyridine dicoumarol derivatives with potential activity against a novel Topoisomerase IIß kinase which was identified in the HIV-1 viral lysate, compounds were designed and synthesized based on a 3D-QSAR study. MATERIALS & METHODS: Based on QSAR model we have designed and synthesized a series of pyridine dicoumarol derivatives and characterized by spectral studies, all the molecules are biologically evaluated by kinase assay, cytotoxicity assay, ELISA and PCR method. RESULT: We demonstrated the achievement of water soluble disodium pyridine dicoumarate derivatives showing high anti-HIV-1 activity (IC <25 nM) which provides a crucial point for further development of pyridine dicoumarol series as HIV-1-associated topoisomerase IIß kinase inhibitors for clinical application against AIDS. CONCLUSION: A new class of anti-HIV-1 lead compounds have been designed and tested. Further studies would result in development of  novel and potential drugs. [Mh] Termos MeSH primário: DNA Topoisomerases Tipo II/metabolismo Proteínas de Ligação a DNA/metabolismo Dicumarol/metabolismo HIV-1/enzimologia Inibidores da Topoisomerase II/metabolismo [Mh] Termos MeSH secundário: Fármacos Anti-HIV/química Fármacos Anti-HIV/metabolismo Fármacos Anti-HIV/toxicidade Linhagem Celular Sobrevivência Celular/efeitos dos fármacos Proteínas de Ligação a DNA/antagonistas & inibidores Dicumarol/química Dicumarol/farmacologia Desenho de Drogas Ensaio de Imunoadsorção Enzimática Proteína do Núcleo p24 do HIV/antagonistas & inibidores Proteína do Núcleo p24 do HIV/metabolismo HIV-1/efeitos dos fármacos Seres Humanos Piridinas/química Relação Quantitativa Estrutura-Atividade Inibidores da Topoisomerase II/química Inibidores da Topoisomerase II/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-HIV Agents); 0 (DNA-Binding Proteins); 0 (HIV Core Protein p24); 0 (Pyridines); 0 (Topoisomerase II Inhibitors); 7QID3E7BG7 (Dicumarol); EC 5.99.1.3 (DNA Topoisomerases, Type II); NH9L3PP67S (pyridine) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170912 [St] Status: MEDLINE [do] DOI: 10.4155/fmc-2017-0091

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[PMID]: 28432147 [Au] Autor: Gutjahr A; Papagno L; Nicoli F; Lamoureux A; Vernejoul F; Lioux T; Gostick E; Price DA; Tiraby G; Perouzel E; Appay V; Verrier B; Paul S [Ad] Endereço: InvivoGen, 31400 Toulouse, France. [Ti] Título: Cutting Edge: A Dual TLR2 and TLR7 Ligand Induces Highly Potent Humoral and Cell-Mediated Immune Responses. [So] Source: J Immunol;198(11):4205-4209, 2017 Jun 01. [Is] ISSN: 1550-6606 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: TLR agonists are currently being developed and tested as adjuvants in various formulations to optimize the immunogenicity and efficacy of vaccines. The aim of this study was to evaluate the immunostimulatory properties of a novel compound incorporating covalently linked moieties designed to stimulate both TLR2 and TLR7. This dual TLR2/TLR7 agonist induced the maturation of dendritic cells and primed substantial populations of cytolytic and highly polyfunctional effector CD8 T cells in vitro, and safely potentiated the immunogenic properties of a nanoparticulate Ag in vivo, eliciting humoral responses with a balanced T 1/T 2 profile in mice. Collectively, these data reveal the potential utility of chimeric adjuvants with synergistic activities mediated via TLRs. [Mh] Termos MeSH primário: Adjuvantes Imunológicos Imunidade Celular Imunidade Humoral Glicoproteínas de Membrana/agonistas Glicoproteínas de Membrana/imunologia Receptor 2 Toll-Like/agonistas Receptor 2 Toll-Like/imunologia Receptor 7 Toll-Like/agonistas Receptor 7 Toll-Like/imunologia [Mh] Termos MeSH secundário: Animais Formação de Anticorpos Linfócitos T CD8-Positivos/imunologia Diferenciação Celular Citocinas Células Dendríticas/imunologia Células Dendríticas/fisiologia Proteína do Núcleo p24 do HIV/administração & dosagem Proteína do Núcleo p24 do HIV/imunologia Ligantes Camundongos Camundongos Endogâmicos C57BL Nanopartículas Proteínas Recombinantes de Fusão/imunologia Vacinação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Adjuvants, Immunologic); 0 (Cytokines); 0 (HIV Core Protein p24); 0 (Ligands); 0 (Membrane Glycoproteins); 0 (Recombinant Fusion Proteins); 0 (Tlr2 protein, mouse); 0 (Tlr7 protein, mouse); 0 (Toll-Like Receptor 2); 0 (Toll-Like Receptor 7) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170423 [St] Status: MEDLINE [do] DOI: 10.4049/jimmunol.1602131

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[PMID]: 28222792 [Au] Autor: Karabaev BB; Beisheeva NJ; Satybaldieva AB; Ismailova AD; Pessler F; Akmatov MK [Ad] Endereço: Republican Blood Centre, Bishkek, Kyrgyzstan, Chingiz Aitmatov Ave 60, 720044, Bishkek, Kyrgyzstan. [Ti] Título: Seroprevalence of hepatitis B, hepatitis C, human immunodeficiency virus, Treponema pallidum, and co-infections among blood donors in Kyrgyzstan: a retrospective analysis (2013-2015). [So] Source: Infect Dis Poverty;6(1):45, 2017 Feb 21. [Is] ISSN: 2049-9957 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Post-Soviet Kyrgyzstan has experienced a major surge in blood-borne infections, but data from adequately powered, up-to-date studies are lacking. We thus examined a) the seroprevalences of hepatitis B virus surface antigen (HBsAg), HIV-1 p24 antigen and antibodies against hepatitis C virus (anti-HCV), human immunodeficiency viruses (anti-HIV-1/2, HIV-1 group O), and Treponema pallidum among blood donors in Kyrgyzstan and assess their distribution according to sex, age, and provinces of residence; b) trends in the respective seroprevalences; and c) co-infection rates among the pathogens studied. METHODS: Serological screening was performed on 37 165 blood donors at the Republican Blood Centre in Bishkek, Kyrgyzstan, between January 2013 and December 2015. We applied poststratification weights to control for sampling bias and used logistic regression analyses to examine the association of seropositivity and co-infections with sex, age, provinces of residence, and year of blood donation. RESULTS: Twenty nine thousand and one hundred forty-five (78%) donors were males and 8 020 (22%) were females. The median age was 27 years (range: 18 - 64). The prevalences of HBsAg, anti-HCV, HIV (p24 Ag and anti-HIV), and anti-T. pallidum were 3.6% (95%CI: 3.4 - 3.8%), 3.1% (3.0 - 3.3%), 0.78% (0.69 - 0.87%), and 3.3% (3.1 - 3.5%), respectively. Males were more likely to be seropositive for HBsAg than females (OR: 1.63; 95%CI: 1.40 - 1.90), but less likely to be seropositive for anti-HCV (0.85; 0.74 - 0.98) and HIV (0.65; 0.49 - 0.85). Prevalences were lower in the capital than in the other provinces. There was a decreasing trend in the seroprevalences of HBsAg, anti-HCV, and anti-T. pallidum from 2012 to 2015 (P-value for trend, P = 0.01, P < 0.0001, P < 0.0001, respectively), while the seroprevalence of HIV increased (P = 0.049). One hundred eighty donors (0.48%) were seropositive for multiple infections. The highest co-infection rate was observed between anti-T. pallidum and HBsAg (6.0%), followed by anti-HCV and anti-T. pallidum (5.2%), and HIV and anti-HCV (4.9%). CONCLUSIONS: The data suggest that Kyrgyzstan can be reclassified from high to lower-intermediate HBsAg endemicity, whereas the high HIV prevalence with a rising trend is an alarming finding that needs to be urgently addressed by public health authorities. The observed co-infections suggest common risk factors but also common preventive interventions. [Mh] Termos MeSH primário: Doadores de Sangue Coinfecção/epidemiologia Infecções por HIV/epidemiologia Hepatite B/epidemiologia Hepatite C/epidemiologia Sífilis/epidemiologia [Mh] Termos MeSH secundário: Adolescente Adulto Anticorpos Antibacterianos/sangue Anticorpos Antivirais/sangue Ensaio de Imunoadsorção Enzimática Feminino Proteína do Núcleo p24 do HIV/sangue Infecções por HIV/imunologia Infecções por HIV/virologia Soroprevalência de HIV HIV-1/imunologia Hepacivirus/imunologia Hepatite B/imunologia Hepatite B/virologia Antígenos de Superfície da Hepatite B/sangue Vírus da Hepatite B/imunologia Hepatite C/imunologia Hepatite C/virologia Anticorpos Anti-Hepatite C/sangue Seres Humanos Quirguistão/epidemiologia Masculino Programas de Rastreamento Meia-Idade Estudos Retrospectivos Estudos Soroepidemiológicos Sífilis/imunologia Sífilis/microbiologia Treponema pallidum/imunologia Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Bacterial); 0 (Antibodies, Viral); 0 (HIV Core Protein p24); 0 (Hepatitis B Surface Antigens); 0 (Hepatitis C Antibodies) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170306 [Lr] Data última revisão: 170306 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170223 [St] Status: MEDLINE [do] DOI: 10.1186/s40249-017-0255-9

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[PMID]: 28199410 [Au] Autor: Kosaka PM; Pini V; Calleja M; Tamayo J [Ad] Endereço: IMM-Instituto de Microelectrónica de Madrid (CNM-CSIC), Isaac Newton, 8, PTM, Tres Cantos, Madrid, Spain. [Ti] Título: Ultrasensitive detection of HIV-1 p24 antigen by a hybrid nanomechanical-optoplasmonic platform with potential for detecting HIV-1 at first week after infection. [So] Source: PLoS One;12(2):e0171899, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Early detection of HIV infection is the best way to prevent spread of the disease and to improve the efficiency of the antiretroviral therapy. Nucleic acid amplification tests (NAAT) have become the gold-standard for detecting low-concentrations of the virus in blood. However, these methods are technically demanding and cost-prohibitive in developing countries. Immunoassays are more affordable and can be more easily adapted for point-of-care diagnosis. However, the sensitivity so far of these methods has been too low. We here report the development of a sandwich immunoassay that combines nanomechanical and optoplasmonic transduction methods for detecting the HIV-1 capsid antigen p24 in human serum. The immunoreactions take place on the surface of a compliant microcantilever where gold nanoparticles are used as both mechanical and plasmonic labels. The microcantilever acts as both a mechanical resonator and an optical cavity for the transduction of the mechanical and plasmonic signals. The limit of detection of the immunoassay is 10-17 g/mL that is equivalent to one virion in 10 mL of plasma. This is 5 orders of magnitude better than last generation of approved immunoassays and 2 orders of magnitude better than NAAT. This technology meets the demands to be produced en masse at low cost and the capability for miniaturization to be used at the point-of-care. [Mh] Termos MeSH primário: Proteína do Núcleo p24 do HIV/sangue Infecções por HIV/diagnóstico HIV-1/metabolismo Imunoensaio/métodos Nanotecnologia [Mh] Termos MeSH secundário: Ouro/química Seres Humanos Imunoensaio/instrumentação Limite de Detecção Masculino Nanopartículas Metálicas/química Microscopia Sistemas Automatizados de Assistência Junto ao Leito Sensibilidade e Especificidade Vírion/química Vírion/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (HIV Core Protein p24); 7440-57-5 (Gold) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170828 [Lr] Data última revisão: 170828 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170216 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0171899

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[PMID]: 28077644 [Au] Autor: Passaes CP; Bruel T; Decalf J; David A; Angin M; Monceaux V; Muller-Trutwin M; Noel N; Bourdic K; Lambotte O; Albert ML; Duffy D; Schwartz O; Sáez-Cirión A; ANRS RHIVIERA Consortium [Ad] Endereço: Institut Pasteur, Unité HIV, Inflammation et Persistance, Départements de Virologie et Immunologie, Paris, France. [Ti] Título: Ultrasensitive HIV-1 p24 Assay Detects Single Infected Cells and Differences in Reservoir Induction by Latency Reversal Agents. [So] Source: J Virol;91(6), 2017 03 15. [Is] ISSN: 1098-5514 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The existence of HIV reservoirs in infected individuals under combined antiretroviral therapy (cART) represents a major obstacle toward cure. Viral reservoirs are assessed by quantification of HIV nucleic acids, a method which does not discriminate between infectious and defective viruses, or by viral outgrowth assays, which require large numbers of cells and long-term cultures. Here, we used an ultrasensitive p24 digital assay, which we report to be 1,000-fold more sensitive than classical enzyme-linked immunosorbent assays (ELISAs) in the quantification of HIV-1 Gag p24 production in samples from HIV-infected individuals. Results from ultrasensitive p24 assays were compared to those from conventional viral RNA reverse transcription-quantitative PCR (RT-qPCR)-based assays and from outgrowth assay readout by flow cytometry. Using serial dilutions and flow-based single-cell sorting, we show that viral proteins produced by a single infected cell can be detected by the ultrasensitive p24 assay. This unique sensitivity allowed the early (as soon as day 1 in 43% of cases) and more efficient detection and quantification of p24 in phytohemagglutinin-L (PHA)-stimulated CD4 T cells from individuals under effective cART. When seven different classes of latency reversal agents (LRA) in resting CD4 T cells from HIV-infected individuals were tested, the ultrasensitive p24 assay revealed differences in the extent of HIV reactivation. Of note, HIV RNA production was infrequently accompanied by p24 protein production (19%). Among the drugs tested, prostratin showed a superior capacity in inducing viral protein production. In summary, the ultrasensitive p24 assay allows the detection and quantification of p24 produced by single infected CD4 T cells and provides a unique tool to assess early reactivation of infectious virus from reservoirs in HIV-infected individuals. The persistence of HIV reservoirs in infected individuals under effective antiretroviral treatment represents a major obstacle toward cure. Different methods to estimate HIV reservoirs exist, but there is currently no optimal assay to measure HIV reservoirs in HIV eradication interventions. In the present study, we report an ultrasensitive digital ELISA platform for quantification of the HIV-1 protein p24. This method was employed to assess the early reactivation of infectious virus from reservoirs in HIV-1-infected individuals. We found that viral proteins produced by a single infected cell can be detected by an ultrasensitive p24 assay. This unprecedented resolution showed major advantages in comparison to other techniques currently used to assess viral replication in reactivation studies. In addition, such a highly sensitive assay allows discrimination of drug-induced reactivation of productive HIV based on protein expression. The present study heralds new opportunities to evaluate the HIV reservoir and the efficacy of drugs used to target it. [Mh] Termos MeSH primário: Proteína do Núcleo p24 do HIV/análise HIV-1/efeitos dos fármacos HIV-1/fisiologia Virologia/métodos Ativação Viral/efeitos dos fármacos Latência Viral/efeitos dos fármacos [Mh] Termos MeSH secundário: Linfócitos T CD4-Positivos/virologia Infecções por HIV/virologia Seres Humanos Sensibilidade e Especificidade [Pt] Tipo de publicação: COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE [Nm] Nome de substância: 0 (HIV Core Protein p24) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170828 [Lr] Data última revisão: 170828 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170113 [St] Status: MEDLINE

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[PMID]: 28073435 [Au] Autor: He X; Wang W; Xu K; Feng X; Zeng Y [Ad] Endereço: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. [Ti] Título: Evaluation of the efficacy of a therapeutic HIV vaccine by in vitro stimulation assay. [So] Source: Cell Immunol;313:67-71, 2017 Mar. [Is] ISSN: 1090-2163 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A novel method for HIV vaccine efficacy evaluation was established and the experimental conditions optimized. This novel method was then applied to determine whether a recombinant adenovirus type 5 HIV therapeutic vaccine expressing Gag antigen (Ad5-HIVgag) could stimulate HIV-specific cellular responses in vitro. The results indicated that HIV-specific IFN-gama production lymphocytes were induced by the Ad5-HIVgag vaccine. Compared with other methods, this in vitro stimulation method is safe and time-efficient, and the result is more intuitive. It has the potential to be regarded as a supplement to other methods for evaluating the IFN-gamma production by PBMCs to HIV vaccination. [Mh] Termos MeSH primário: Vacinas contra a AIDS/farmacologia Infecções por HIV/terapia HIV-1/imunologia Imunoterapia/métodos Linfócitos T/imunologia [Mh] Termos MeSH secundário: Vacinas contra a AIDS/genética Vacinas contra Adenovirus Adolescente Adulto Células Cultivadas China ELISPOT Feminino Proteína do Núcleo p24 do HIV/genética Infecções por HIV/imunologia Seres Humanos Interferon gama/metabolismo Ativação Linfocitária Masculino Meia-Idade Fragmentos de Peptídeos/genética Adulto Jovem [Pt] Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE [Nm] Nome de substância: 0 (AIDS Vaccines); 0 (Adenovirus Vaccines); 0 (HIV Core Protein p24); 0 (Peptide Fragments); 82115-62-6 (Interferon-gamma) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170705 [Lr] Data última revisão: 170705 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170112 [St] Status: MEDLINE

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[PMID]: 28060886 [Au] Autor: Meggi B; Bollinger T; Mabunda N; Vubil A; Tobaiwa O; Quevedo JI; Loquiha O; Vojnov L; Peter TF; Jani IV [Ad] Endereço: Instituto Nacional de Saúde, Maputo, Mozambique. [Ti] Título: Point-Of-Care p24 Infant Testing for HIV May Increase Patient Identification despite Low Sensitivity. [So] Source: PLoS One;12(1):e0169497, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The long delay in returning test results during early infant diagnosis of HIV (EID) often causes loss-to-follow-up prior to antiretroviral treatment (ART) initiation in resource-limited settings. A point-of-care (POC) test may help overcome these challenges. We evaluated the performance of the LYNX p24 Antigen POC test in Mozambique. 879 HIV-exposed infants under 18 months of age were enrolled consecutively at three primary healthcare clinics (PHC). Lancet heel-drawn blood was tested on-site by nurses using a prototype POC test for HIV Gag p24 antigen detection. Results of POC testing were compared to laboratory-based nucleic acid testing on dried blood spots. A comparison of the effect of sensitivity and timely test results return on successful diagnosis by POC and laboratory-based platforms was also calculated. The sensitivity and specificity of the LYNX p24 Ag test were 71.9%; (95% confidence interval [CI]: 58.5-83.0%) and 99.6% (95% CI: 98.9-99.9%), respectively. The predictive value of positive and negative tests were 93.2% (95% CI: 81.3-98.6%) and 97.9% (95% CI: 96.8-98.8%), respectively. Overall agreement was high (Cohen Kappa = 0.80; 95% CI: 0.71-0.89). Despite its lower sensitivity, the POC test had the potential to provide test results to up to 81% more patients compared to the laboratory-based test. This prototype POC p24 assay was feasible for use in PHCs but demonstrated low sensitivity for HIV detection. POC EID technologies that perform below standard recommendations may still be valuable diagnostic tools in settings with inefficient EID networks. [Mh] Termos MeSH primário: Proteína do Núcleo p24 do HIV Infecções por HIV/diagnóstico HIV-1 Testes Imediatos [Mh] Termos MeSH secundário: Fármacos Anti-HIV/uso terapêutico Pré-Escolar Estudos Transversais Diagnóstico Precoce Feminino Proteína do Núcleo p24 do HIV/imunologia Infecções por HIV/tratamento farmacológico Infecções por HIV/epidemiologia Infecções por HIV/imunologia HIV-1/imunologia Seres Humanos Lactente Recém-Nascido Masculino Moçambique/epidemiologia Testes Imediatos/normas Reprodutibilidade dos Testes Sensibilidade e Especificidade Padrão de Cuidado Fluxo de Trabalho [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-HIV Agents); 0 (HIV Core Protein p24) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170815 [Lr] Data última revisão: 170815 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170107 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0169497

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[PMID]: 27272704 [Au] Autor: Fitzgerald N; Cross M; O'Shea S; Fox J [Ad] Endereço: Department of Genito-Urinary Medicine, Guy's and St Thomas' NHS Foundation Trust, London, UK. [Ti] Título: Diagnosing acute HIV infection at point of care: a retrospective analysis of the sensitivity and specificity of a fourth-generation point-of-care test for detection of HIV core protein p24. [So] Source: Sex Transm Infect;93(2):100-101, 2017 Mar. [Is] ISSN: 1472-3263 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: OBJECTIVES: Detection of acute HIV infection is vital in preventing onward transmission. HIV point-of-care testing (POCT) has improved uptake of HIV testing but has been limited to third-generation assays, which only detect chronic HIV infection. Previous evaluation of the fourth-generation Alere Determine HIV-1/2 Ag/Ab Combo POCT showed only 50% sensitivity for HIV core protein p24 (p24 antigen) detection, which is suboptimal for diagnosis of acute HIV infection with limited advantage over third-generation POCT. We aimed to assess the sensitivity of the new Alere HIV Combo POCT to detect acute HIV infection. METHODS: Stored samples in samples already identified as p24-positive using standard-of-care fourth-generation assays were randomly selected alongside groups of antibody-positive samples and HIV-negative samples. Each sample was tested using the new Alere POCT according to manufacturer's instructions. Sensitivity and specificity were then calculated. RESULTS: The Alere HIV Combo POCT test demonstrated 88% sensitivity 95% CI (78% to 98%) and 100% specificity 95% CI (99.7% to 100%) for detection of p24 antigen. CONCLUSIONS: This new POCT shows improved sensitivity for detection of p24 antigen and may be of value for clinical use in detecting acute HIV infection. Further evaluation of its use in a clinical setting is still required. [Mh] Termos MeSH primário: Anticorpos Anti-HIV/sangue Proteína do Núcleo p24 do HIV/sangue Infecções por HIV/diagnóstico HIV-1/imunologia Técnicas Imunoenzimáticas/métodos Testes Imediatos [Mh] Termos MeSH secundário: Anticorpos Anti-HIV/análise Proteína do Núcleo p24 do HIV/análise Seres Humanos Programas de Rastreamento Kit de Reagentes para Diagnóstico Estudos Retrospectivos Sensibilidade e Especificidade Reino Unido [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (HIV Antibodies); 0 (HIV Core Protein p24); 0 (Reagent Kits, Diagnostic) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170607 [Lr] Data última revisão: 170607 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160609 [St] Status: MEDLINE [do] DOI: 10.1136/sextrans-2015-052491

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[PMID]: 27264875 [Au] Autor: Ogasawara N; Takano KI; Kobayashi H; Kikuchi K; Matsumiya H; Yoshioka I; Himi T [Ad] Endereço: Department of Otorhinolaryngology, Sapporo Medical University School of Medicine, Sapporo, Japan; Department of Otorhinolaryngology, Obihiro Kousei Hospital, Japan. Electronic address: ogasawara.n@sapmed.ac.jp. [Ti] Título: HIV-associated cystic lesions of the parotid gland. [So] Source: Auris Nasus Larynx;44(1):126-130, 2017 Feb. [Is] ISSN: 1879-1476 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: We present two cases of an HIV-associated parotid gland cyst. One case was a 36-year-old HIV infected woman. She was diagnosed with HIV infection and presented with slowly enlarged parotid gland cysts together with elevation of HIV viral RNA copies/mL in her serum. She was performed parotid gland biopsy under the general anesthesia. The histopathologic analysis revealed negative HIV p24-antigen in her parotid gland tissue. The other case was a 43-year-old man found his parotid gland swelling shortly after highly active antiretroviral therapy (HAART). He was diagnosed with HIV infection 2 years previously. He had started HAART several days before. He showed exceeding elevation of IgE in his serum. We treated him with medication using anti-histamic drugs for his cyst. A computed tomography scan revealed a complete response of his parotid gland cyst 4 weeks after the medication. His serum IgE level was decreased to half of the level before the medication. These findings suggested that the parotid gland swelling associated with HIV was due to various factors including immune reconstitution inflammatory syndrome (IRIS). In case such a parotid gland swelling, we could avoid invasive treatments. [Mh] Termos MeSH primário: Cistos/imunologia Infecções por HIV/imunologia Síndrome Inflamatória da Reconstituição Imune/imunologia Imunoglobulina E/imunologia Doenças Parotídeas/imunologia [Mh] Termos MeSH secundário: Adulto Terapia Antirretroviral de Alta Atividade Cistos/complicações Cistos/diagnóstico por imagem Cistos/tratamento farmacológico Feminino Proteína do Núcleo p24 do HIV/metabolismo Infecções por HIV/complicações Infecções por HIV/tratamento farmacológico Infecções por HIV/metabolismo Antagonistas dos Receptores Histamínicos/uso terapêutico Seres Humanos Masculino Doenças Parotídeas/complicações Doenças Parotídeas/diagnóstico por imagem Doenças Parotídeas/tratamento farmacológico RNA Viral/metabolismo Tomografia Computadorizada por Raios X [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 0 (HIV Core Protein p24); 0 (Histamine Antagonists); 0 (RNA, Viral); 37341-29-0 (Immunoglobulin E) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170723 [Lr] Data última revisão: 170723 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160607 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 27998072 [Au] Autor: Sepúlveda-Crespo D; Vacas-Córdoba E; Márquez-Miranda V; Araya-Durán I; Gómez R; Mata FJ; González-Nilo FD; Muñoz-Fernández MÁ [Ad] Endereço: Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank , Madrid 28007, Spain. [Ti] Título: Effect of Several HIV Antigens Simultaneously Loaded with G2-NN16 Carbosilane Dendrimer in the Cell Uptake and Functionality of Human Dendritic Cells. [So] Source: Bioconjug Chem;27(12):2844-2849, 2016 Dec 21. [Is] ISSN: 1520-4812 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Dendrimers are highly branched, star-shaped, and nanosized polymers that have been proposed as new carriers for specific HIV-1 peptides. Dendritic cells (DCs) are the most-potent antigen-presenting cells that play a major role in the development of cell-mediated immunotherapy due to the generation and regulation of adaptive immune responses against HIV-1. This article reports on the associated behavior of two or three HIV-derived peptides simultaneously (p24/gp160 or p24/gp160/NEF) with cationic carbosilane dendrimer G2-NN16. We have found that (i) immature DCs (iDCs) and mature (mDCs) did not capture efficiently HIV peptides regarding the uptake level when cells were treated with G2-NN16-peptide complex alone; (ii) the ability of DCs to migrate was not depending on the peptides presence; and (iii) with the use of molecular dynamic simulation, a mixture of peptides decreased the cell uptake of the other peptides (in particular, NEF hinders the binding of more peptides and is especially obstructing of the binding of gp160 to G2-NN16). The results suggest that G2-NN16 cannot be considered as an alternative carrier for delivering two or more HIV-derived peptides to DCs. [Mh] Termos MeSH primário: Dendrímeros/química Células Dendríticas/efeitos dos fármacos Antígenos HIV/química Silanos/química [Mh] Termos MeSH secundário: Dendrímeros/farmacocinética Antígenos HIV/farmacologia Proteína do Núcleo p24 do HIV/química Proteína gp160 do Envelope de HIV/química Seres Humanos Simulação de Dinâmica Molecular Silanos/farmacocinética Eletricidade Estática [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (2G-NN16); 0 (Dendrimers); 0 (HIV Antigens); 0 (HIV Core Protein p24); 0 (HIV Envelope Protein gp160); 0 (Silanes); 0 (carbosilane) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170605 [Lr] Data última revisão: 170605 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161222 [St] Status: MEDLINE [do] DOI: 10.1021/acs.bioconjchem.6b00623

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