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[PMID]:28464897
[Au] Autor:Arzt J; Pacheco JM; Stenfeldt C; Rodriguez LL
[Ad] Endereço:Foreign Animal Disease Research Unit, Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, NY, USA. Jonathan.Arzt@ars.usda.gov.
[Ti] Título:Pathogenesis of virulent and attenuated foot-and-mouth disease virus in cattle.
[So] Source:Virol J;14(1):89, 2017 05 02.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Understanding the mechanisms of attenuation and virulence of foot-and-mouth disease virus (FMDV) in the natural host species is critical for development of next-generation countermeasures such as live-attenuated vaccines. Functional genomics analyses of FMDV have identified few virulence factors of which the leader proteinase (L ) is the most thoroughly investigated. Previous work from our laboratory has characterized host factors in cattle inoculated with virulent FMDV and attenuated mutant strains with transposon insertions within L . METHODS: In the current study, the characteristics defining virulence of FMDV in cattle were further investigated by comparing the pathogenesis of a mutant, attenuated strain (FMDV-Mut) to the parental, virulent virus from which the mutant was derived (FMDV-WT). The only difference between the two viruses was an insertion mutation in the inter-AUG region of the leader proteinase of FMDV-Mut. All cattle were infected by simulated-natural, aerosol inoculation. RESULTS: Both viruses were demonstrated to establish primary infection in the nasopharyngeal mucosa with subsequent dissemination to the lungs. Immunomicroscopic localization of FMDV antigens indicated that both viruses infected superficial epithelial cells of the nasopharynx and lungs. The critical differences between the two viruses were a more rapid establishment of infection by FMDV-WT and quantitatively greater virus loads in secretions and infected tissues compared to FMDV-Mut. The slower replicating FMDV-Mut established a subclinical infection that was limited to respiratory epithelial sites, whereas the faster replication of FMDV-WT facilitated establishment of viremia, systemic dissemination of infection, and clinical disease. CONCLUSION: The mutant FMDV was capable of achieving all the same early pathogenesis landmarks as FMDV-WT, but was unable to establish systemic infection. The precise mechanism of attenuation remains undetermined; but current data suggests that the impaired replication of the mutant is more responsible for attenuation than differences in host immunological factors. These results complement previous studies by providing data of high-granularity describing tissue-specific tropism of FMDV and by demonstrating microscopic localization of virulent and attenuated clones of the same field-strain FMDV.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Vírus da Febre Aftosa/patogenicidade
Febre Aftosa/virologia
Virulência
[Mh] Termos MeSH secundário: Aerossóis
Animais
Bovinos
Células Epiteliais/patologia
Células Epiteliais/virologia
Febre Aftosa/imunologia
Febre Aftosa/patologia
Vírus da Febre Aftosa/genética
Vírus da Febre Aftosa/crescimento & desenvolvimento
Vírus da Febre Aftosa/isolamento & purificação
Pulmão/virologia
Mutagênese Insercional
Nasofaringe/patologia
Nasofaringe/virologia
RNA Viral/isolamento & purificação
Vacinas Atenuadas/imunologia
Proteínas Estruturais Virais
Fatores de Virulência
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Aerosols); 0 (RNA, Viral); 0 (Vaccines, Attenuated); 0 (Viral Structural Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0758-9


  2 / 5515 MEDLINE  
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[PMID]:29267277
[Au] Autor:Um J; Chun BC; Lee YS; Hwang KJ; Yang DK; Park JS; Kim SY
[Ad] Endereço:Division of Zoonoses, Center for Immunology & Pathology, Korea National Institute of Health, Cheongju-si, Chungcheongbuk-do, Republic of Korea.
[Ti] Título:Development and evaluation of an anti-rabies virus phosphoprotein-specific monoclonal antibody for detection of rabies neutralizing antibodies using RFFIT.
[So] Source:PLoS Negl Trop Dis;11(12):e0006084, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rabies is a major public health problem with a fatality rate close to 100%; however, complete prevention can be achieved through pre- or post-exposure prophylaxis. The rapid fluorescent focus inhibition test (RFFIT) is one of the recommended testing methods to determine the production of neutralizing antibodies after vaccination. Here, we report the development of a new monoclonal antibody (mAb) designed to react specifically with Rabies virus (RABV) phosphoprotein (P protein), and the evaluation of its applicability to the RFFIT and its effectiveness as a diagnostic reagent for human rabies. METHODOLOGY/PRINCIPAL FINDINGS: The mAb KGH P 16B8 was produced to target the P protein of the Korean KGH RABV strain. An indirect immunofluorescence assay (IFA) was conducted to detect various strains of RABV in various cell lines. Alexa-conjugated KGH P 16B8 (16B8-Alexa) was developed for the RFFIT. The IFA test could detect RABV up to a 1:2,500 dilution, with a detection limit comparable to that of a commercial diagnostic reagent. The sensitivity, specificity, positive predictive value, and negative predictive value of the RFFIT using 16B8-Alexa in 414 clinical specimens were 98.67%, 99.47%, 99.55%, and 98.42%, respectively. The results of the RFFIT with 16B8-Alexa were strongly correlated with those obtained using an existing commercial diagnostic reagent (r = 0.995, p<0.001). CONCLUSIONS/SIGNIFICANCE: The mAb developed in this study shows high sensitivity and specificity, confirming its clinical utility with the RFFIT to measure the rabies neutralizing antibody titer and establish a diagnosis in human. Thus, 16B8-Alexa is expected to serve as an alternative diagnostic reagent that is widely accessible, with potentially broad applications beyond those of the RFFIT in Korea. Further studies with 16B8-Alexa should provide insight into the immunological mechanism of the P protein of Korean RABV.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Técnica Indireta de Fluorescência para Anticorpo/métodos
Testes de Neutralização/métodos
Fosfoproteínas/imunologia
Vírus da Raiva/imunologia
Raiva/prevenção & controle
Proteínas Estruturais Virais/imunologia
[Mh] Termos MeSH secundário: Anticorpos Neutralizantes/imunologia
Anticorpos Antivirais/imunologia
Profilaxia Pós-Exposição/métodos
Raiva/virologia
Vacinas Antirrábicas/imunologia
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (P phosphoprotein, Rabies virus); 0 (Phosphoproteins); 0 (Rabies Vaccines); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006084


  3 / 5515 MEDLINE  
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[PMID]:28989096
[Au] Autor:Wussow F; Chiuppesi F; Meng Z; Martinez J; Nguyen J; Barry PA; Diamond DJ
[Ad] Endereço:Department of Experimental Therapeutics, Beckman Research Institute of the City of Hope, Duarte, CA, USA. Electronic address: fwussow@coh.org.
[Ti] Título:Exploiting 2A peptides to elicit potent neutralizing antibodies by a multi-subunit herpesvirus glycoprotein complex.
[So] Source:J Virol Methods;251:30-37, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Neutralizing antibodies (NAb) interfering with glycoprotein complex-mediated virus entry into host cells are thought to contribute to the protection against herpesvirus infection. However, using herpesvirus glycoprotein complexes as vaccine antigens can be complicated by the necessity of expressing multiple subunits simultaneously to allow efficient complex assembly and formation of conformational NAb epitopes. By using a novel bacterial artificial chromosome (BAC) clone of the clinically deployable Modified Vaccinia Ankara (MVA) vector and exploiting ribosomal skipping mediated by 2A peptides, MVA vectors were generated that expressed self-processing subunits of the human cytomegalovirus (HCMV) pentamer complex (PC) composed of gH, gL, UL128, UL130, and UL131A. These MVA vectors expressed 2A-linked HCMV PC subunits that were efficiently cleaved and transported to the cell surface as protein complexes forming conformational neutralizing epitopes. In addition, vaccination of mice by only two immunizations with these MVA vectors resulted in potent HCMV NAb responses that remained stable over a period of at least six months. This method of eliciting NAb by 2A-linked, self-processing HCMV PC subunits could contribute to develop a HCMV vaccine candidate and may serve as a template to facilitate the development of subunit vaccine strategies against other herpesviruses.
[Mh] Termos MeSH primário: Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Antígenos Virais/imunologia
Vacinas contra Citomegalovirus/imunologia
Citomegalovirus/imunologia
Glicoproteínas/imunologia
Proteínas Estruturais Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/genética
Citomegalovirus/genética
Vacinas contra Citomegalovirus/administração & dosagem
Vacinas contra Citomegalovirus/genética
Vetores Genéticos
Glicoproteínas/genética
Esquemas de Imunização
Camundongos
Vacinas Sintéticas/administração & dosagem
Vacinas Sintéticas/genética
Vacinas Sintéticas/imunologia
Vírus Vaccinia/genética
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Cytomegalovirus Vaccines); 0 (Glycoproteins); 0 (Vaccines, Synthetic); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  4 / 5515 MEDLINE  
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[PMID]:29048274
[Au] Autor:Kobayashi D; Isawa H; Fujita R; Murota K; Itokawa K; Higa Y; Katayama Y; Sasaki T; Mizutani T; Iwanaga S; Ohta N; Garcia-Bertuso A; Sawabe K
[Ad] Endereço:1​Department of Environmental Parasitology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
[Ti] Título:Isolation and characterization of a new iflavirus from Armigeres spp. mosquitoes in the Philippines.
[So] Source:J Gen Virol;98(11):2876-2881, 2017 Nov.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30 nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~10 p.f.u. ml ) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.
[Mh] Termos MeSH primário: Culicidae/virologia
Vírus dos Insetos/classificação
Vírus dos Insetos/isolamento & purificação
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Filipinas
Ensaio de Placa Viral
Proteínas Estruturais Virais/análise
Vírion/química
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000929


  5 / 5515 MEDLINE  
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[PMID]:29035172
[Au] Autor:Liu YT; Jiang J; Bohannon KP; Dai X; Gant Luxton GW; Hui WH; Bi GQ; Smith GA; Hong Zhou Z
[Ad] Endereço:2​California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA.
[Ti] Título:A pUL25 dimer interfaces the pseudorabies virus capsid and tegument.
[So] Source:J Gen Virol;98(11):2837-2849, 2017 Nov.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inside the virions of α-herpesviruses, tegument protein pUL25 anchors the tegument to capsid vertices through direct interactions with tegument proteins pUL17 and pUL36. In addition to promoting virion assembly, both pUL25 and pUL36 are critical for intracellular microtubule-dependent capsid transport. Despite these essential roles during infection, the stoichiometry and precise organization of pUL25 and pUL36 on the capsid surface remain controversial due to the insufficient resolution of existing reconstructions from cryo-electron microscopy (cryoEM). Here, we report a three-dimensional (3D) icosahedral reconstruction of pseudorabies virus (PRV), a varicellovirus of the α-herpesvirinae subfamily, obtained by electron-counting cryoEM at 4.9 Å resolution. Our reconstruction resolves a dimer of pUL25 forming a capsid-associated tegument complex with pUL36 and pUL17 through a coiled coil helix bundle, thus correcting previous misinterpretations. A comparison between reconstructions of PRV and the γ-herpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) reinforces their similar architectures and establishes important subfamily differences in the capsid-tegument interface.
[Mh] Termos MeSH primário: Herpesvirus Suídeo 1/química
Herpesvirus Suídeo 1/ultraestrutura
Multimerização Proteica
Proteínas Estruturais Virais/análise
Proteínas Estruturais Virais/ultraestrutura
Vírion/química
Vírion/ultraestrutura
[Mh] Termos MeSH secundário: Microscopia Crioeletrônica
Imagem Tridimensional
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171114
[Lr] Data última revisão:
171114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000903


  6 / 5515 MEDLINE  
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[PMID]:28988058
[Au] Autor:Zhang L; Li H; Chen Y; Gao X; Lu Z; Gao L; Wang Y; Gao Y; Gao H; Liu C; Cui H; Zhang Y; Pan Q; Qi X; Wang X
[Ad] Endereço:Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, PR China.
[Ti] Título:The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection.
[So] Source:Virology;512:211-221, 2017 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infectious bursal disease virus (IBDV) is an important immunosuppressive virus of chickens. Although the gene functions of IBDV have been well characterized, the host responses during IBDV infection remain much poor. In the present study, casein kinase 1 alpha (CK1α), a novel VP2-associated protein, was down-regulated during IBDV replication in DF1 cells. Further experiments showed that siRNA-mediated knockdown of CK1α inhibited IBDV replication, while overexpression of CK1α promoted IBDV growth. Finally, we revealed that the effects of CK1α expression level on IBDV replication were involved in the negative regulation of CK1α on type I interferon receptor (IFNAR1), because ubiquitination assay analyses demonstrated that CK1α could promote the ubiquitination of IFNAR1, thereby affecting the stability of this receptor. In conclusion, down-regulation of CK1α during IBDV infection as a host defense response increased abundance of IFNAR1, which in turn enhanced an inhibitory effect on IBDV replication.
[Mh] Termos MeSH primário: Caseína Quinase I/metabolismo
Fibroblastos/metabolismo
Regulação Enzimológica da Expressão Gênica/imunologia
Vírus da Doença Infecciosa da Bursa/fisiologia
[Mh] Termos MeSH secundário: Animais
Caseína Quinase I/genética
Linhagem Celular
Embrião de Galinha
Regulação para Baixo
Ligação Proteica
Receptor de Interferon alfa e beta/genética
Receptor de Interferon alfa e beta/metabolismo
Proteínas Estruturais Virais/genética
Proteínas Estruturais Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (VP2 protein, infectious bursal disease virus); 0 (Viral Structural Proteins); 156986-95-7 (Receptor, Interferon alpha-beta); EC 2.7.11.1 (Casein Kinase I)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  7 / 5515 MEDLINE  
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[PMID]:28902862
[Au] Autor:Kaida A; Iritani N; Yamamoto SP; Kanbayashi D; Hirai Y; Togawa M; Amo K; Kohdera U; Nishigaki T; Shiomi M; Asai S; Kageyama T; Kubo H
[Ad] Endereço:Division of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
[Ti] Título:Distinct genetic clades of enterovirus D68 detected in 2010, 2013, and 2015 in Osaka City, Japan.
[So] Source:PLoS One;12(9):e0184335, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The first upsurge of enterovirus D68 (EV-D68), a causative agent of acute respiratory infections (ARIs), in Japan was reported in Osaka City in 2010. In this study, which began in 2010, we surveyed EV-D68 in children with ARIs and analyzed sequences of EV-D68 strains detected. Real-time PCR of 19 respiratory viruses or subtypes of viruses, including enterovirus, was performed on 2,215 specimens from ARI patients (<10 years of age) collected between November 2010 and December 2015 in Osaka City, Japan. EV-D68 was identified in 18 enterovirus-positive specimens (n = 4 in 2013, n = 1 in 2014, and n = 13 in 2015) by analysis of viral protein 1 (VP1) or VP4 sequences, followed by a BLAST search for similar sequences. All EV-D68 strains were detected between June and October (summer to autumn), except for one strain detected in 2014. A phylogenetic analysis of available VP1 sequences revealed that the Osaka strains detected in 2010, 2013, and 2015 belonged to distinct clusters (Clades C, A, and B [Subclade B3], respectively). Comparison of the 5' untranslated regions of these viruses showed that Osaka strains in Clades A, B (Subclade B3), and C commonly had deletions at nucleotide positions 681-703 corresponding to the prototype Fermon strain. Clades B and C had deletions from nucleotide positions 713-724. Since the EV-D68 epidemic in 2010, EV-D68 re-emerged in Osaka City, Japan, in 2013 and 2015. Results of this study indicate that distinct clades of EV-D68 contributed to re-emergences of this virus in 2010, 2013, and 2015 in this limited region.
[Mh] Termos MeSH primário: Enterovirus Humano D/classificação
Enterovirus Humano D/genética
Infecções por Enterovirus/epidemiologia
Infecções por Enterovirus/virologia
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
Doenças Transmissíveis Emergentes/virologia
Surtos de Doenças/estatística & dados numéricos
Feminino
Seres Humanos
Lactente
Recém-Nascido
Japão/epidemiologia
Masculino
Filogenia
Infecções Respiratórias/virologia
Análise de Sequência de DNA
Urbanização
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184335


  8 / 5515 MEDLINE  
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Alfieri, Alice Fernandes
Alfieri, Amauri Alcindo
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[PMID]:28849283
[Au] Autor:Ribeiro J; Lorenzetti E; Júnior JCR; da Silva Medeiros TN; Alfieri AF; Alfieri AA
[Ad] Endereço:Laboratory of Animal Virology, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil.
[Ti] Título:Phylogenetic analysis of VP1 and RdRP genes of Brazilian aichivirus B strains involved in a diarrhea outbreak in dairy calves.
[So] Source:Arch Virol;162(12):3691-3696, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Aichivirus B has been reported worldwide in calves and adult cattle with and without diarrhea. The aim of this study was to describe the molecular characteristics of the RdRP and VP1 genes of aichivirus B strains identified as the most frequent etiologic agent in a neonatal diarrhea outbreak in a high-production Brazilian dairy cattle herd. Preliminary laboratory analysis ruled out important enteropathogens (Cryptosporidium spp; Eimeria spp., E. coli F5, and bovine coronavirus). Fecal samples from diarrheic (n = 24) and asymptomatic (n = 5) calves up to 30 days old were collected for virological analysis. RT-PCR assays were performed for the detection of aichivirus B RdRP and VP1 genes and for rotavirus A VP7 and VP4 genes in fecal samples. Asymptomatic calves (control group) were negative for both viruses. Aichivirus B and rotavirus A G10P[11] genotypes were found in 54.2% (13/24) and 25% (6/24) of the diarrheic fecal samples, respectively. Aichivirus B was only identified (83.3%, 10/12) in calves up to two weeks old. Phylogenetic analysis based on the RdRP gene grouped the Brazilian strains in a new branch within the aichivirus B group. Comparative analysis of the nucleotide sequence of the VP1 gene of Brazilian and Chinese aichivirus B strains allowed the strains identified in this study to be classified in the putative lineage 1. This is the first description of a high rate of aichivirus B detection in a diarrhea outbreak in dairy calves, and the first phylogenetic study of the VP1 gene of aichivirus B wild-type strains performed in South America.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Diarreia/veterinária
Surtos de Doenças
Kobuvirus/classificação
Kobuvirus/isolamento & purificação
Filogenia
Infecções por Picornaviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Brasil/epidemiologia
Bovinos
Doenças dos Bovinos/epidemiologia
Diarreia/epidemiologia
Diarreia/virologia
Kobuvirus/genética
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/virologia
RNA Replicase/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins); EC 2.7.7.48 (RNA Replicase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3531-x


  9 / 5515 MEDLINE  
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[PMID]:28825213
[Au] Autor:Michel LO; Jackwood DJ
[Ad] Endereço:Food Animal Health Research Program, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH, 44691, USA.
[Ti] Título:Classification of infectious bursal disease virus into genogroups.
[So] Source:Arch Virol;162(12):3661-3670, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Infectious bursal disease virus (IBDV) causes infectious bursal disease (IBD), an immunosuppressive disease of poultry. The current classification scheme of IBDV is confusing because it is based on antigenic types (variant and classical) as well as pathotypes. Many of the amino acid changes differentiating these various classifications are found in a hypervariable region of the capsid protein VP2 (hvVP2), the major host protective antigen. Data from this study were used to propose a new classification scheme for IBDV based solely on genogroups identified from phylogenetic analysis of the hvVP2 of strains worldwide. Seven major genogroups were identified, some of which are geographically restricted and others that have global dispersion, such as genogroup 1. Genogroup 2 viruses are predominately distributed in North America, while genogroup 3 viruses are most often identified on other continents. Additionally, we have identified a population of genogroup 3 vvIBDV isolates that have an amino acid change from alanine to threonine at position 222 while maintaining other residues conserved in this genogroup (I242, I256 and I294). A222T is an important mutation because amino acid 222 is located in the first of four surface loops of hvVP2. A similar shift from proline to threonine at 222 is believed to play a role in the significant antigenic change of the genogroup 2 IBDV strains, suggesting that antigenic drift may be occurring in genogroup 3, possibly in response to antigenic pressure from vaccination.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Genótipo
Vírus da Doença Infecciosa da Bursa/classificação
Vírus da Doença Infecciosa da Bursa/genética
Doenças das Aves Domésticas/virologia
Proteínas Estruturais Virais/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/virologia
Saúde Global
Filogeografia
Aves Domésticas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (VP2 protein, infectious bursal disease virus); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3500-4


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[PMID]:28800730
[Au] Autor:Yilmaz H; Karakullukcu A; Turan N; Cizmecigil UY; Yilmaz A; Ozkul AA; Aydin O; Gunduz A; Mete M; Zeyrek FY; Kirazoglu TT; Richt JA; Kocazeybek B
[Ad] Endereço:Department of Virology, Veterinary Faculty, University of Istanbul, Avcilar, Istanbul, Turkey. hyilmaz@istanbul.edu.tr.
[Ti] Título:Genotypes of hepatitis a virus in Turkey: first report and clinical profile of children infected with sub-genotypes IA and IIIA.
[So] Source:BMC Infect Dis;17(1):561, 2017 Aug 11.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hepatitis A virus (HAV) is a food and water-borne virus causing clinical (mainly hepatitis) and subclinical disease in humans. It is important to characterize circulating strains of HAV in order to prevent HAV infections using efficacious vaccines. The aim of this study was the detection and characterization of the circulating strains of HAV in Turkey by performing serology, RT-PCR, sequencing and phylogenetic analysis. METHODS: In this study, 355 HAV suspected cases were analysed by ELISA for the presence of antibodies to HAV. RNA was extracted from 54 HAV IgM positive human sera. None of the suspect cases were vaccinated against HAV and they never received blood transfusions. Samples found positive by RT-PCR using primers targeting the VP1/VP2A junction and VP1/VP3 capsid region of HAV, were subjected to sequencing and phylogenetic analyses. RESULTS: IgM type antibodies to HAV were detected in 54 patients. Twenty one of them were students. The age of IgM positive cases was between 3 and 60 years. IgM positivity differed in age groups and was higher in the age group 3 to 10 years. Phylogenetic analysis showed that the majority of HAV strains detected in this study belong to the "HAV 1B" cluster. In addition, the HAV sub-genotypes IA (KT874461.1) and IIIA (KT222963.1) were found in 2 children. These sub-genotypes were not previously reported in Turkey. The child who carried sub-genotype IIIA travelled to Afghanistan and presented with abdominal pain, icterus and vomitus. He was positive for anti-HAV IgM and IgG but negative for hepatitis B and C. Liver enzymes like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase and lactate dehydrogenase were severely elevated. Bilirubin levels were also increased. White blood cells, neutrophils and hemoglobin were decreased while lymphocytes and monocytes were increased. Similar clinical signs and laboratory findings were reported for the child infected with sub-genotype IA but aspartate aminotransferase and alanine aminotransferase were not severely elevated. CONCLUSIONS: The results indicate that molecular studies determining the HAV genotype variation in Turkey are timely and warranted. The majority of IgM positive cases in 3-10 year old patients indicate that childhood vaccination is important. Sub-genotype IB is the most prevalant genotype in Turkey. Surprisingly, sub-genotype IA and IIIA are also present in Turkey; future diagnostic efforts need to include diagnostic methods which can identify this emerging HAV genotypes. Our results also show that one important risk factor for contracting hepatitis A virus is international travel since genotype IIIA was detected in a child who had travelled to Afghanistan.
[Mh] Termos MeSH primário: Vírus da Hepatite A/genética
Hepatite A/etiologia
Filogenia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Afeganistão
Criança
Pré-Escolar
Feminino
Genótipo
Hepatite A/virologia
Anticorpos Anti-Hepatite A/sangue
Vírus da Hepatite A/isolamento & purificação
Vírus da Hepatite A/patogenicidade
Seres Humanos
Fígado/enzimologia
Fígado/virologia
Masculino
Meia-Idade
Turquia
Proteínas Estruturais Virais/genética
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hepatitis A Antibodies); 0 (VP1 protein, hepatitis A virus); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2667-3



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