Base de dados : MEDLINE Pesquisa : D13.150.650.319.500 [Categoria DeCS] Referências encontradas : 4 [refinar] Mostrando: 1 .. 4   no formato [Detalhado]

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MEDLINE

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[PMID]: 28460582 [Au] Autor: Iguchi T; Niino N; Tamai S; Sakurai K; Mori K [Ad] Endereço: 1 Medicinal Safety Research Laboratories, Daiichi Sankyo Co, Ltd, Tokyo, Japan. [Ti] Título: Comprehensive Analysis of Circulating microRNA Specific to the Liver, Heart, and Skeletal Muscle of Cynomolgus Monkeys. [So] Source: Int J Toxicol;36(3):220-228, 2017 May/Jun. [Is] ISSN: 1092-874X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Circulating microRNAs (miRNAs) could represent sensitive and specific biomarkers for tissue injury. However, their utility as biomarkers in nonclinical toxicological studies using nonhuman primates is limited by a lack of information on their organ specificity and circulating levels under resting condition of the animals. Herein, liver, heart, and skeletal muscle-specific expression patterns of miRNAs were determined in 27 tissues/organs from male and female monkeys (n =2/sex) by next-generation sequencing (NGS) analysis. This analysis revealed organ-specific miRNAs in the liver (miR-122), heart (miR-208a and miR-499a), and skeletal muscle (miR-206). Next, plasma was collected from conscious-naive male and female cynomolgus monkeys (n = 25/sex) to better understand the expressions of organ-specific circulating miRNAs. The absolute values of circulating miRNAs were quantified using a Taqman microRNA assay. MiR-1, miR-133a, and miR-208b showed preferential expression in the heart and skeletal muscles, whereas miR-192 was abundant in the liver, stomach, small intestine, and kidney. These miRNAs had identical sequences to their human counterparts. Six organ-specific miRNAs (miR-1, miR-122, miR-133a, miR-192, miR-206, and miR-499a) could be evaluated quantitatively by quantitative real-time reverse transcription polymerase chain reaction with or without preamplification. No significant sex differences were noted for these circulating miRNAs. For their circulation levels, miR-133a showed more than 900-fold interindividual variation, whereas miR-122 showed only a 20-fold variation. In conclusion, we profiled circulating organ-specific miRNAs for the liver, heart, and skeletal muscle of cynomolgus monkeys. [Mh] Termos MeSH primário: MicroRNA Circulante/genética Fígado/metabolismo Músculo Esquelético/metabolismo Miocárdio/metabolismo [Mh] Termos MeSH secundário: Animais Biomarcadores/sangue Biomarcadores/metabolismo MicroRNA Circulante/sangue Feminino Traumatismos Cardíacos/sangue Traumatismos Cardíacos/genética Fígado/lesões Macaca fascicularis Masculino Músculo Esquelético/lesões Reação em Cadeia da Polimerase em Tempo Real Análise de Sequência de RNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Circulating MicroRNA) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180302 [Lr] Data última revisão: 180302 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170503 [St] Status: MEDLINE [do] DOI: 10.1177/1091581817704975

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MEDLINE

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[PMID]: 29390402 [Au] Autor: Jiang Z; Ma J; Wang Q; Wu F; Ping J; Ming L [Ad] Endereço: Department of Clinical Laboratory, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. [Ti] Título: Circulating microRNA expression and their target genes in deep vein thrombosis: A systematic review and bioinformatics analysis. [So] Source: Medicine (Baltimore);96(50):e9330, 2017 Dec. [Is] ISSN: 1536-5964 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Clinically, D-dimer is the only established biomarker for the diagnosis of deep vein thrombosis (DVT). However, low specificity discounts its diagnostic value. Several publications have illustrated the differentially expressed circulating microRNAs (miRNAs) and their potential diagnostic values for DVT patients. Therefore, we systematically evaluated present researches and further performed bioinformatics analysis, to provide new insights into the diagnosis and underlying mechanisms of miRNAs in DVT. METHODS: Databases PubMed, Web of Science, and Embase were searched from January 2000 to April 2017. Articles on circulating miRNAs expression in DVT were retrieved and reference lists were handpicked. Bioinformatics analysis was conducted for further evaluation. RESULTS: Eventually, the eligibility criteria for inclusion in this study were met by 3 articles, which consisted of 13 specially expressed miRNAs and 149 putative target genes. Two representative KEGG pathways, vascular endothelial growth factor and phosphatidylinositol 3'-kinase (PI3K)-Akt signaling pathway, seemed to participate in the regulatory network of thrombosis. CONCLUSIONS: Despite the potential diagnostic value and regulation effect, the results of circulating miRNAs used as biomarkers for DVT are not so encouraging. More in-depth and larger sample investigations are needed to explore the diagnostic and therapeutic values of miRNAs for DVT. [Mh] Termos MeSH primário: MicroRNA Circulante/genética Trombose Venosa/genética [Mh] Termos MeSH secundário: Biomarcadores/sangue Biologia Computacional Seres Humanos [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Biomarkers); 0 (Circulating MicroRNA) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180301 [Lr] Data última revisão: 180301 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 180203 [St] Status: MEDLINE [do] DOI: 10.1097/MD.0000000000009330

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MEDLINE

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[PMID]: 29187262 [Au] Autor: Wang YN; Chen ZH; Chen WC [Ad] Endereço: The First Affiliated Hospital of Soochow University, Shizi Street #188, Suzhou, 215006, Jiangsu, People's Republic of China. [Ti] Título: Novel circulating microRNAs expression profile in colon cancer: a pilot study. [So] Source: Eur J Med Res;22(1):51, 2017 Nov 29. [Is] ISSN: 2047-783X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: PURPOSE: To identify the expression profile of novel microRNAs (miRNAs) in colon cancer and evaluate their clinical applicability. METHODS: Differences in the expression of serum miRNAs in patients with colon cancer and healthy controls were identified using miRNA microarrays. Differentially expressed miRNAs were verified via real-time polymerase chain reaction (PCR) using sera from 50 patients with colon cancer and 44 healthy controls. These miRNAs were also verified in a double-blind validation experiment using sera from 30 patients with colon cancer, 30 patients with colonic polyps, and 30 healthy controls. RESULTS: Microarray hybridization revealed that 87 miRNAs were differentially expressed between the sera of patients with colon cancer and healthy controls. Among these miRNAs, 39 miRNAs were up-regulated, whereas 48 miRNAs were down-regulated. Verification of the expression of these miRNAs using real-time PCR revealed that the expression levels of miR-31, miR-141, miR-224-3p, miR-576-5p, and miR-4669 were significantly different between patients with colon cancer and healthy controls. Using these five miRNAs to construct a miRNA expression profile (or miRNA panel) will facilitate more effective diagnosis of colon cancer. CONCLUSION: Clinical analysis of miR-31, miR-141, miR-224-3p, miR-576-5p, and miR-4669 expression in patients with colon cancer may facilitate the diagnosis of colon cancer. [Mh] Termos MeSH primário: Biomarcadores Tumorais/sangue Biomarcadores Tumorais/genética MicroRNA Circulante/sangue Neoplasias do Colo/genética [Mh] Termos MeSH secundário: Adulto Idoso Idoso de 80 Anos ou mais Área Sob a Curva Neoplasias do Colo/sangue Método Duplo-Cego Feminino Perfilação da Expressão Gênica Seres Humanos Masculino Meia-Idade Projetos Piloto Curva ROC Sensibilidade e Especificidade Transcriptoma [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers, Tumor); 0 (Circulating MicroRNA) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171201 [St] Status: MEDLINE [do] DOI: 10.1186/s40001-017-0294-5

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MEDLINE

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[PMID]: 29299981 [Au] Autor: Zalewski K; Misiek M; Kowalik A; Bakula-Zalewska E; Kopczynski J; Zielinska A; Bidzinski M; Radziszewski J; Gózdz S; Kowalewska M [Ad] Endereço: 1 Department of Gynecologic Oncology, Holycross Cancer Center, Kielce, Poland. [Ti] Título: Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma. [So] Source: Tumour Biol;39(11):1010428317717140, 2017 Nov. [Is] ISSN: 1423-0380 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The role of circulating microRNAs as a promising tool for diagnosing cancer and monitoring anticancer therapies has been widely studied in the past decades. To date, no suitable reference microRNAs for normalizing quantitative real-time polymerase chain reaction assays has been identified in vulvar intraepithelial neoplasia lesions and vulvar squamous cell carcinoma. The purpose of this study was to select appropriate references for gene expression studies in plasma of patients with these lesions. Expression levels of six microRNAs-hsa-miR-425-5p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-5p, hsa-miR-103a-3p, and hsa-miR-16-5p-were analyzed by quantitative reverse transcription polymerase chain reaction in plasma samples obtained from 17 patients with vulvar intraepithelial neoplasia lesion and 27 patients with vulvar squamous cell carcinoma. The expression stability of these candidate normalizers was assayed using geNorm algorithm. hsa-miR-93-5p was revealed as the most stably expressed reference in plasma samples of both vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients. The results pointed at hsa-miR-93-5p and hsa-miR-425-5p as microRNAs that retained the greatest robustness in plasma of vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients, respectively. Our work is the first report on reference microRNA selection for quantitative real-time polymerase chain reaction applications in vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma. The candidate microRNA stability values for the two types of lesions are provided and might serve for normalization of the future novel microRNA biomarkers in these rare entities. [Mh] Termos MeSH primário: Carcinoma in Situ/genética Carcinoma de Células Escamosas/genética MicroRNA Circulante/genética Regulação Neoplásica da Expressão Gênica Neoplasias Vulvares/genética [Mh] Termos MeSH secundário: Idoso Idoso de 80 Anos ou mais Carcinoma in Situ/sangue Carcinoma de Células Escamosas/sangue MicroRNA Circulante/sangue Feminino Perfilação da Expressão Gênica/métodos Perfilação da Expressão Gênica/normas Seres Humanos MicroRNAs/sangue MicroRNAs/genética Meia-Idade Valores de Referência Neoplasias Vulvares/sangue [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Circulating MicroRNA); 0 (MIRN103 microRNA, human); 0 (MIRN16 microRNA, human); 0 (MIRN191 microRNA, human); 0 (MIRN423 microRNA, human); 0 (MIRN425 microRNA, human); 0 (MIRN93 microRNA, human); 0 (MicroRNAs) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180118 [Lr] Data última revisão: 180118 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180105 [St] Status: MEDLINE [do] DOI: 10.1177/1010428317717140

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