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[PMID]: | 28460582 |
[Au] Autor: | Iguchi T; Niino N; Tamai S; Sakurai K; Mori K |
[Ad] Endereço: | 1 Medicinal Safety Research Laboratories, Daiichi Sankyo Co, Ltd, Tokyo, Japan. |
[Ti] Título: | Comprehensive Analysis of Circulating microRNA Specific to the Liver, Heart, and Skeletal Muscle of Cynomolgus Monkeys. |
[So] Source: | Int J Toxicol;36(3):220-228, 2017 May/Jun. | [Is] ISSN: | 1092-874X |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Circulating microRNAs (miRNAs) could represent sensitive and specific biomarkers for tissue injury. However, their utility as biomarkers in nonclinical toxicological studies using nonhuman primates is limited by a lack of information on their organ specificity and circulating levels under resting condition of the animals. Herein, liver, heart, and skeletal muscle-specific expression patterns of miRNAs were determined in 27 tissues/organs from male and female monkeys (n =2/sex) by next-generation sequencing (NGS) analysis. This analysis revealed organ-specific miRNAs in the liver (miR-122), heart (miR-208a and miR-499a), and skeletal muscle (miR-206). Next, plasma was collected from conscious-naive male and female cynomolgus monkeys (n = 25/sex) to better understand the expressions of organ-specific circulating miRNAs. The absolute values of circulating miRNAs were quantified using a Taqman microRNA assay. MiR-1, miR-133a, and miR-208b showed preferential expression in the heart and skeletal muscles, whereas miR-192 was abundant in the liver, stomach, small intestine, and kidney. These miRNAs had identical sequences to their human counterparts. Six organ-specific miRNAs (miR-1, miR-122, miR-133a, miR-192, miR-206, and miR-499a) could be evaluated quantitatively by quantitative real-time reverse transcription polymerase chain reaction with or without preamplification. No significant sex differences were noted for these circulating miRNAs. For their circulation levels, miR-133a showed more than 900-fold interindividual variation, whereas miR-122 showed only a 20-fold variation. In conclusion, we profiled circulating organ-specific miRNAs for the liver, heart, and skeletal muscle of cynomolgus monkeys. |
[Mh] Termos MeSH primário: |
MicroRNA Circulante/genética Fígado/metabolismo Músculo Esquelético/metabolismo Miocárdio/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Biomarcadores/sangue Biomarcadores/metabolismo MicroRNA Circulante/sangue Feminino Traumatismos Cardíacos/sangue Traumatismos Cardíacos/genética Fígado/lesões Macaca fascicularis Masculino Músculo Esquelético/lesões Reação em Cadeia da Polimerase em Tempo Real Análise de Sequência de RNA
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Biomarkers); 0 (Circulating MicroRNA) |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180302 |
[Lr] Data última revisão:
| 180302 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170503 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1177/1091581817704975 |
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