Base de dados : MEDLINE
Pesquisa : D13.444 [Categoria DeCS]
Referências encontradas : 9100 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 910 ir para página                         

  1 / 9100 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29363539
[Au] Autor:Pirmohamed M
[Ad] Endereço:Department of Molecular and Clinical Pharmacology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK.
[Ti] Título:Nucleic acid based therapies: developing frontier for precision medicine.
[So] Source:BMJ;360:k223, 2018 01 23.
[Is] ISSN:1756-1833
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Terapia Genética/utilização
Terapia de Alvo Molecular/utilização
Ácidos Nucleicos/uso terapêutico
Medicina de Precisão/métodos
[Mh] Termos MeSH secundário: Adenoviridae/genética
Aminofenóis/uso terapêutico
Agonistas dos Canais de Cloreto/uso terapêutico
Fibrose Cística/tratamento farmacológico
Fibrose Cística/genética
Regulador de Condutância Transmembrana em Fibrose Cística/efeitos dos fármacos
Regulador de Condutância Transmembrana em Fibrose Cística/genética
Terapia Genética/economia
Genômica
Hemofilia A/classificação
Hemofilia A/tratamento farmacológico
Hemofilia A/genética
Seres Humanos
Doença dos Neurônios Motores/tratamento farmacológico
Doença dos Neurônios Motores/genética
Mutação
Oligonucleotídeos Antissenso/economia
Oligonucleotídeos Antissenso/uso terapêutico
Quinolonas/uso terapêutico
Reino Unido/epidemiologia
[Pt] Tipo de publicação:EDITORIAL
[Nm] Nome de substância:
0 (Aminophenols); 0 (CFTR protein, human); 0 (Chloride Channel Agonists); 0 (Nucleic Acids); 0 (Oligonucleotides, Antisense); 0 (Quinolones); 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator); 1Y740ILL1Z (ivacaftor)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1136/bmj.k223


  2 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29293661
[Au] Autor:Monson EA; Crosse KM; Das M; Helbig KJ
[Ad] Endereço:Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, Victoria.
[Ti] Título:Lipid droplet density alters the early innate immune response to viral infection.
[So] Source:PLoS One;13(1):e0190597, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cellular localisation of many innate signalling events following viral infection has yet to be elucidated, however there has been a few cases in which membranes of certain cellular organelles have acted as platforms to these events. Of these, lipid droplets (LDs) have recently been identified as signalling platforms for innate TLR7 and 9 signalling. Despite their wide range of similar roles in various metabolic pathways, LDs have been overlooked as potential platforms for antiviral innate signalling events. This study established an in vitro model to evaluate the efficiency of the early innate immune response in cells with reduced LD content to the viral mimics, dsDNA and dsRNA, and Sendai viral infection. Using RT-qPCR, the expression of IFN-ß and IFN-λ was quantified following stimulation along with the expression of specific ISGs. Luciferase based assays evaluated the combined expression of ISRE-promoter driven ISGs under IFN-ß stimulation. Cellular LD content did not alter the entry of fluorescently labelled viral mimics into cells, but significantly decreased the ability of both Huh-7 and HeLa cells to produce type I and III IFN, as well as downstream ISG expression, indicative of an impeded innate immune response. This observation was also seen during Sendai virus infection of HeLa cells, where both control and LD reduced cells replicated the virus to the same level, but a significantly impaired type I and III IFN response was observed in the LD reduced cells. In addition to altered IFN production, cells with reduced LD content exhibited decreased expression of specific antiviral ISGs: Viperin, IFIT-1 and OAS-1 under IFN-ß stimulation; However the overall induction of the ISRE-promoter was not effected. This study implicates a role for LDs in an efficient early innate host response to viral infection and future work will endeavour to determine the precise role these important organelles play in induction of an antiviral response.
[Mh] Termos MeSH primário: Imunidade Inata
Gotículas Lipídicas/metabolismo
Viroses/imunologia
[Mh] Termos MeSH secundário: Western Blotting
Linhagem Celular
Meios de Cultura
Seres Humanos
Interferon Tipo I/imunologia
Ácidos Nucleicos/metabolismo
RNA de Cadeia Dupla/imunologia
Reação em Cadeia da Polimerase em Tempo Real
Vírus Sendai/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (Interferon Type I); 0 (Nucleic Acids); 0 (RNA, Double-Stranded)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190597


  3 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28463658
[Au] Autor:Jiang X; Lv B; Wang Y; Shen Q; Wang X
[Ad] Endereço:1​Department of Emergency, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, PR China.
[Ti] Título:Bactericidal mechanisms and effector targets of TiO2 and  Ag-TiO2 against Staphylococcus aureus.
[So] Source:J Med Microbiol;66(4):440-446, 2017 Apr.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: In our previous study, Ag+-loaded TiO2 and Ag+-loaded SiO2 coatings for tracheal intubation were prepared to prevent ventilator-associated pneumonia (VAP), but the antimicrobial targets and the underlying mechanisms of TiO2 and Ag-TiO2 (Ag+) are still unclear. We attempted to elucidate the antimicrobial activity and potential mechanisms against Staphylococcus aureus. METHODOLOGY: The study tested the TiO2 and Ag+ bacteriostatic activity against S. aureus strains by MIC assays and S. aureus growth curves, lesion in the membranes by surface hydrophobicity tests, conductivity tests and measurements of DNA and RNA contents in S. aureus cultures, and investigated the inhibition of soluble protein and nucleic acid synthesis by measurements of soluble protein content, fluorescent intensity and nucleic acid content of living S. aureus. RESULTS: The MIC values of TiO2 and Ag+ were 1.6 mg ml-1 and 5.781 µg ml-1. TiO2 and Ag+ could inhibit the growth of S. aureus. After treatment with TiO2 and Ag+, the surface hydrophobicity was significantly reduced, the conductivity of cultures increased, and DNA and RNA content in cultures showed no obvious changes. The expressions of soluble proteins and nucleic acid contents of living S. aureus were reduced after treatment with TiO2 and Ag+. CONCLUSION: TiO2 and Ag+ could cause slight lesion in the membrane to affect S. aureus membrane permeability, but not decomposition of membrane. Moreover, TiO2 and Ag+ could lead to reduced expression of soluble protein by inhibiting the synthesis of nucleic acids, thereby further inhibiting the growth of S. aureus.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Pneumonia Associada à Ventilação Mecânica/prevenção & controle
Prata/farmacologia
Infecções Estafilocócicas/prevenção & controle
Staphylococcus aureus/crescimento & desenvolvimento
Propriedades de Superfície/efeitos dos fármacos
Titânio/farmacologia
[Mh] Termos MeSH secundário: Interações Hidrofóbicas e Hidrofílicas
Testes de Sensibilidade Microbiana
Ácidos Nucleicos/biossíntese
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Nucleic Acids); 15FIX9V2JP (titanium dioxide); 3M4G523W1G (Silver); D1JT611TNE (Titanium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000457


  4 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470515
[Au] Autor:Chan LL; McCulley KJ; Kessel SL
[Ad] Endereço:Department of Technology R&D, Nexcelom Bioscience LLC, 360 Merrimack Street, Building 9, Lawrence, MA, 01843, USA. lchan@nexcelom.com.
[Ti] Título:Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry.
[So] Source:Methods Mol Biol;1601:27-41, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to accurately measure cell viability is important for any cell-based assay. Traditionally, viability measurements have been performed using the trypan blue exclusion method on a hemacytometer, which allows researchers to visually distinguish viable from nonviable cells. While the trypan blue method can work for cell lines or primary cells that have been rigorously purified, in more complex samples such as PBMCs, bone marrow, whole blood, or any sample with low viability, this method can lead to errors. In recent years, advances in optics and fluorescent dyes have led to the development of automated benchtop image-based cell counters for rapid cell concentration and viability measurement. In this work, we demonstrate the use of image-based cytometry for cell viability detection using single-, dual-, or multi-stain techniques. Single-staining methods using nucleic acid stains such as EB, PI, 7-AAD, DAPI, SYTOX Green, and SYTOX Red, and enzymatic stains such as CFDA and Calcein AM, were performed. Dual-staining methods using AO/PI, CFDA/PI, Calcein AM/PI, Hoechst/PI, Hoechst/DRAQ7, and DRAQ5/DAPI that enumerate viable and nonviable cells were also performed. Finally, Hoechst/Calcein AM/PI was used for a multi-staining method. Fluorescent viability staining allows exclusion of cellular debris and nonnucleated cells from analysis, which can eliminate the need to perform purification steps during sample preparation and improve efficiency. Image cytometers increase speed and throughput, capture images for visual confirmation of results, and can greatly simplify cell count and viability measurements.
[Mh] Termos MeSH primário: Sobrevivência Celular
Citometria por Imagem/métodos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Contagem de Células
Corantes Fluorescentes/química
Células HeLa
Seres Humanos
Células Jurkat
Células MCF-7
Ácidos Nucleicos/química
Fatores de Tempo
Azul Tripano/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Nucleic Acids); I2ZWO3LS3M (Trypan Blue)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_3


  5 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28742856
[Au] Autor:Yakovleva A; Plieskatt JL; Jensen S; Humeida R; Lang J; Li G; Bracci P; Silver S; Bethony JM
[Ad] Endereço:Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Washington DC, United States of America.
[Ti] Título:Fit for genomic and proteomic purposes: Sampling the fitness of nucleic acid and protein derivatives from formalin fixed paraffin embedded tissue.
[So] Source:PLoS One;12(7):e0181756, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The demand for nucleic acid and protein derivatives from formalin-fixed paraffin-embedded (FFPE) tissue has greatly increased due to advances in extraction and purification methods, making these derivatives available for numerous genomic and proteomic platforms. Previously, DNA, RNA, microRNA (miRNA), or protein derived from FFPE tissue blocks were considered "unfit" for such platforms, as the process of tissue immobilization by FFPE resulted in cross-linked, fragmented, and chemically modified macromolecules. We conducted a systematic examination of nucleic acids and proteins co-extracted from 118 FFPE blocks sampled from the AIDS and Cancer Specimen Resource (ACSR) at The George Washington University after stratification by storage duration and the three most common tumor tissue types at the ACSR (adenocarcinoma, squamous cell carcinoma, and papillary carcinoma). DNA, RNA, miRNA, and protein could be co-extracted from 98% of the FFPE blocks sampled, with DNA and miRNA "fit" for diverse genomic purposes including sequencing. While RNA was the most labile of the FFPE derivatives, especially when assessed by RNA integrity number (RIN), it was still "fit" for genomic methods that use smaller sequence lengths, e.g., quantitative PCR. While more than half of the protein derivatives were fit for proteomic purposes, our analyses indicated a significant interaction effect on the absorbance values for proteins derived from FFPE, implying that storage duration may affect protein derivatives differently by tumor tissue type. The mean absorbance value for proteins derived from more recently stored FFPE was greater than protein derived from older FFPE, with the exception of adenocarcinoma tissue. Finally, the fitness of one type of derivative was weakly associated with the fitness of derivatives co-extracted from the same FFPE block. The current study used several novel quality assurance approaches and metrics to show that archival FFPE tissue blocks are a valuable resource for contemporary genomic and proteomic platforms.
[Mh] Termos MeSH primário: Genômica/métodos
Ácidos Nucleicos/análise
Inclusão em Parafina/métodos
Proteínas/análise
Proteômica/métodos
[Mh] Termos MeSH secundário: Adenocarcinoma/química
Adenocarcinoma/genética
Carcinoma Papilar/química
Carcinoma Papilar/genética
Carcinoma de Células Escamosas/química
Carcinoma de Células Escamosas/genética
DNA/análise
DNA/genética
DNA de Neoplasias/análise
DNA de Neoplasias/genética
Formaldeído/uso terapêutico
Seres Humanos
MicroRNAs/análise
MicroRNAs/genética
Ácidos Nucleicos/genética
Proteínas/genética
RNA/análise
RNA/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Neoplasm); 0 (MicroRNAs); 0 (Nucleic Acids); 0 (Proteins); 1HG84L3525 (Formaldehyde); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181756


  6 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29319937
[Au] Autor:Food and Drug Administration, HHS.
[Ti] Título:Medical Devices; Clinical Chemistry and Clinical Toxicology Devices; Classification of the Reagents for Molecular Diagnostic Instrument Test Systems. Final order.
[So] Source:Fed Regist;82(247):61162-3, 2017 Dec 27.
[Is] ISSN:0097-6326
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Food and Drug Administration (FDA or we) is classifying the reagents for molecular diagnostic instrument test systems into class I (general controls). We are taking this action because we have determined that classifying the device into class I (general controls) will provide a reasonable assurance of safety and effectiveness of the device. We believe this action will also enhance patients' access to beneficial innovative devices, in part by reducing regulatory burdens.
[Mh] Termos MeSH primário: Testes de Química Clínica/classificação
Testes de Química Clínica/instrumentação
Segurança de Equipamentos/classificação
Indicadores e Reagentes/classificação
Biologia Molecular/classificação
Biologia Molecular/instrumentação
Kit de Reagentes para Diagnóstico/classificação
[Mh] Termos MeSH secundário: DNA Polimerase Dirigida por DNA/classificação
Seres Humanos
Ácidos Nucleicos/classificação
Nucleotídeos/classificação
DNA Polimerase Dirigida por RNA/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Nucleic Acids); 0 (Nucleotides); 0 (Reagent Kits, Diagnostic); EC 2.7.7.49 (RNA-Directed DNA Polymerase); EC 2.7.7.7 (DNA-Directed DNA Polymerase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:T
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


  7 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28741501
[Au] Autor:Ntwasa M
[Ad] Endereço:Department of Life and Consumer Sciences, Calabash Building, Cnr Christiaan de Wet & Pioneer Roads, Florida 1710, Republic of South Africa. Electronic address: ntwasmm@unisa.ac.za.
[Ti] Título:Retinoblastoma Binding Protein 6, Another p53 Monitor.
[So] Source:Trends Cancer;2(11):635-637, 2016 Nov.
[Is] ISSN:2405-8025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The retinoblastoma binding protein 6 (RBBP6), a p53 negative regulator, is essential for embryonic development. Its loss-of-function phenotype is similar to mouse double minute homolog (MDM2), the prototypical negative regulator of p53. This article draws attention to the molecular and biological functions of RBBP6 and to its association with carcinogenesis.
[Mh] Termos MeSH primário: Carcinogênese/metabolismo
Proteínas de Transporte/metabolismo
Proteínas de Ligação a DNA/metabolismo
[Mh] Termos MeSH secundário: Animais
Ciclo Celular
Diferenciação Celular
Proliferação Celular
Desenvolvimento Embrionário
Seres Humanos
Ácidos Nucleicos/metabolismo
Proteína Supressora de Tumor p53/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (DNA-Binding Proteins); 0 (Nucleic Acids); 0 (RBBP6 protein, human); 0 (Tumor Suppressor Protein p53)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  8 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29188993
[Au] Autor:Santiana JJ; Sui B; Gomez N; Rouge JL
[Ad] Endereço:Department of Chemistry, University of Connecticut , Storrs, Connecticut 06269, United States.
[Ti] Título:Programmable Peptide-Cross-Linked Nucleic Acid Nanocapsules as a Modular Platform for Enzyme Specific Cargo Release.
[So] Source:Bioconjug Chem;28(12):2910-2914, 2017 Dec 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Herein we describe a modular assembly strategy for photo-cross-linking peptides into nucleic acid functionalized nanocapsules. The peptides embedded within the nanocapsules form discrete nanoscale populations capable of gating the release of molecular and nanoscale cargo using enzyme-substrate recognition as a triggered release mechanism. Using photocatalyzed thiol-yne chemistry, different peptide cross-linkers were effectively incorporated into the nanocapsules and screened against different proteases to test for degradation specificity both in vitro and in cell culture. By using a combination of fluorescence assays, confocal and TEM microscopy, the particles were shown to be highly specific for their enzyme targets, even between enzymes of similar protease classes. The rapid and modular nature of the assembly strategy has the potential to be applied to both intracellular and extracellular biosensing and drug delivery applications.
[Mh] Termos MeSH primário: Portadores de Fármacos/química
Liberação Controlada de Fármacos
Metaloproteinase 9 da Matriz/metabolismo
Nanocápsulas/química
Ácidos Nucleicos/química
Peptídeos/química
[Mh] Termos MeSH secundário: Azidas/química
Transporte Biológico
Enflurano/química
Ouro/química
Ouro/metabolismo
Células HeLa
Seres Humanos
Nanopartículas Metálicas
Compostos de Sulfidrila/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (Drug Carriers); 0 (Nanocapsules); 0 (Nucleic Acids); 0 (Peptides); 0 (Sulfhydryl Compounds); 7440-57-5 (Gold); 91I69L5AY5 (Enflurane); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00629


  9 / 9100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28450169
[Au] Autor:Simão Carlos MI; Zheng K; Garrett N; Arifin N; Workman DG; Kubajewska I; Halwani AA; Moger J; Zhang Q; Schätzlein AG; Uchegbu IF
[Ad] Endereço:School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK.
[Ti] Título:Limiting the level of tertiary amines on polyamines leads to biocompatible nucleic acid vectors.
[So] Source:Int J Pharm;526(1-2):106-124, 2017 Jun 30.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We have designed an efficient, synthetic nucleic acid vector, which is relatively non-toxic. [N-(2-ethylamino)-6-O-glycolchitosan - EAGC] polymers were 10-50 fold less toxic than Lipofectamine 2000, able to complex DNA, mRNA and siRNA into positively charged (zeta potential=+40 - 50mV), 50-450nm nanoparticles. The level of tertiary amine N-2-ethylamino substitution (DS ) was inversely proportional to the IC50 of the EAGC polymers in the A431 cell line: IC50=6.18DS , r =0.9991. EAGC polyplexes were stable against a heparin challenge, able to protect the nucleic acids from nuclease degradation and achieve levels of transfection comparable to Lipofectamine 2000 formulations. The relative biocompatibility of the vector allowed 10 fold higher doses of DNA (1µg compared to 0.1µg per well with Lipofectamine 2000) and siRNA (10.7µg per well vs 1.3µg with Lipofectamine 2000) to be applied to cells, when compared to Lipofectamine 2000. Finally intranasal application of EAGC - siRNA complexes resulted in siRNA transfer to the neurons of the olfactory bulb.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Vetores Genéticos/química
Ácidos Nucleicos/química
Poliaminas/química
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Sobrevivência Celular
DNA
Seres Humanos
Lipídeos
RNA Mensageiro
RNA Interferente Pequeno
Ratos Sprague-Dawley
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Lipids); 0 (Lipofectamine); 0 (Nucleic Acids); 0 (Polyamines); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 9007-49-2 (DNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  10 / 9100 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28465627
[Au] Autor:Falzone M; Crespo E; Jones K; Khan G; Korn VL; Patel A; Patel M; Patel K; Perkins C; Siddiqui S; Stenger D; Yu E; Gelber M; Scheffler R; Nayda V; Ravin A; Komal R; Rudolf JD; Shen B; Gullo V; Demain AL
[Ad] Endereço:Research Institute of Scientists Emeriti (RISE), Charles A. Dana Research Institute, Drew University, Madison, NJ, USA.
[Ti] Título:Nutritional control of antibiotic production by Streptomyces platensis MA7327: importance of l-aspartic acid.
[So] Source:J Antibiot (Tokyo);70(7):828-831, 2017 Jul.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Streptomyces platensis MA7327 is a bacterium producing interesting antibiotics, which act by the novel mechanism of inhibiting fatty acid biosynthesis. The antibiotics produced by this actinomycete are platensimycin and platencin plus some minor related antibiotics. Platensimycin and platencin have activity against antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus; they also lack toxicity in animal models. Platensimycin also has activity against diabetes in a mouse model. We have been interested in studying the effects of primary metabolites on production of these antibiotics in our chemically defined production medium. In the present work, we tested 32 primary metabolites for their effect. They included 20 amino acids, 7 vitamins and 5 nucleic acid derivatives. Of these, only l-aspartic acid showed stimulation of antibiotic production. We conclude that the stimulatory effect of aspartic acid is due to its role as a precursor involved in the biosynthesis of aspartate-4-semialdehyde, which is the starting point for the biosynthesis of the 3-amino-2,4-dihydroxy benzoic acid portion of the platensimycin molecule.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Ácido Aspártico/administração & dosagem
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Adamantano/isolamento & purificação
Aminoácidos/administração & dosagem
Aminoácidos/metabolismo
Aminobenzoatos/isolamento & purificação
Aminofenóis/isolamento & purificação
Anilidas/isolamento & purificação
Antibacterianos/biossíntese
Ácido Aspártico/química
Ácidos Nucleicos/administração & dosagem
Ácidos Nucleicos/metabolismo
Compostos Policíclicos/isolamento & purificação
Vitaminas/administração & dosagem
Vitaminas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Aminobenzoates); 0 (Aminophenols); 0 (Anilides); 0 (Anti-Bacterial Agents); 0 (Nucleic Acids); 0 (Polycyclic Compounds); 0 (Vitamins); 0 (platencin); 30KYC7MIAI (Aspartic Acid); PJY633525U (Adamantane); Q3DQ78KOFY (platensimycin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.49



página 1 de 910 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde