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[PMID]:29458669
[Au] Autor:Barcyte D; Hodac L; Nedbalová L; Elster J
[Ad] Endereço:1​Department of Ecology, Faculty of Science, Charles University, Vinicná 7, Prague 2, 128 44, Czech Republic.
[Ti] Título:Chloromonas arctica sp. nov., a psychrotolerant alga from snow in the High Arctic (Chlamydomonadales, Chlorophyta).
[So] Source:Int J Syst Evol Microbiol;68(3):851-859, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With the advent of molecular phylogenetic methods, it has become possible to assess the bioversity of snow algae more accurately. In this study, we focused on a morphological, ultrastructural and taxonomic description of a new Chloromonas-like alga isolated from snow in the High Arctic (Svalbard). Light and transmission electron microscopy revealed broad ellipsoidal or ellipsoidal-cylindrical, occasionally spherical cells with a chloroplast without a pyrenoid, an inconspicuous eyespot and a papilla. The size difference and the aforementioned morphological traits clearly distinguished the alga from its closest counterparts within the genus Chloromonas. Moreover, we were able to cultivate the alga at both 5 and 20 °C, revealing the psychrotolerant nature of the strain. Phylogenetic analyses of the plastid rbcL and nuclear 18S rRNA gene showed that the alga is nested within a clade containing a number of psychrotolerant strains within the Chloromonadinia phylogroup (Chlorophyceae). In the rbcL phylogeny, the alga formed an independent lineage, sister to the freshwater species Chloromonas paraserbinowii. Comparisons of secondary structure models of a highly variable ITS2 rDNA marker showed support for a distinct species identity for the new strain. The ITS2 secondary structure of the new isolate differed from the closest matches 'Chlamydomonas' gerloffii and Choloromonas reticulata by three and five compensatory base changes, respectively. Considering the morphological and molecular differences from its closest relatives, a new psychrotolerant species from the Arctic, Choromonas arctica sp. nov., is proposed.
[Mh] Termos MeSH primário: Filogenia
Neve
Volvocida/classificação
[Mh] Termos MeSH secundário: DNA de Algas/genética
DNA Espaçador Ribossômico/genética
Plastídeos/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
Svalbard
Volvocida/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002595


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[PMID]:29017964
[Au] Autor:Ishinishi R; Matsuura H; Tanaka S; Nozawa S; Tanada K; Kawashita N; Fujiyama K; Miyasaka H; Hirata K
[Ad] Endereço:Applied Environmental Biology Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
[Ti] Título:Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga.
[So] Source:Gene;640:14-20, 2018 Jan 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The genetic basis of stress resistance in extremophilic microalgae is not well studied. In this study, a gene of unknown function, the cluster58 or CL58 gene, was identified from the halotolerant green alga Chlamydomonas W80 and characterized. The CL58 gene encodes a protein containing a domain of unknown function, the CHRD domain, and a putative secretory signaling sequence at its N-terminus. The levels of CL58 mRNA increased in response to high copper levels and low temperatures. When the CL58 gene was heterologously expressed as a fusion gene with the NanoLuc luciferase gene in Chlamydomonas reinhardtii, a majority of the NanoLuc activity was detected in the culture medium compared with that in the intracellular fraction. A mutagenic analysis revealed that the putative secretory signaling sequence was sufficient for the secretion of the CL58-NanoLuc fusion protein. In addition, we expressed the protein encoded by the CL58 gene in Escherichia coli; the recombinant, soluble protein was then purified. In summary, we identified a novel gene from C. W80 that appears to encode a stress-responsive, CHRD domain-containing secreted protein.
[Mh] Termos MeSH primário: Chlamydomonas reinhardtii/genética
DNA de Algas/genética
Glicoproteínas/química
Peptídeos e Proteínas de Sinalização Intercelular/química
Proteínas de Plantas/genética
Estresse Fisiológico/genética
Transgenes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Chlamydomonas reinhardtii/crescimento & desenvolvimento
Chlamydomonas reinhardtii/metabolismo
Temperatura Baixa
Cobre/toxicidade
Proteínas de Plantas/metabolismo
Domínios Proteicos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (Glycoproteins); 0 (Intercellular Signaling Peptides and Proteins); 0 (Plant Proteins); 0 (Recombinant Proteins); 789U1901C5 (Copper); 93586-27-7 (chordin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE


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Ribeiro, Márcio Garcia
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[PMID]:28884665
[Au] Autor:Morandi S; Cremonesi P; Povolo M; Capra E; Silvetti T; Castiglioni B; Ribeiro MG; Alves AC; da Costa GM; Luini M; Brasca M
[Ad] Endereço:1​Institute of Sciences of Food Production, Italian National Research Council (CNR ISPA), Via Celoria 2, 20133 Milan, Italy.
[Ti] Título:Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk.
[So] Source:Int J Syst Evol Microbiol;67(10):3865-3871, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strain of an achlorophyllic alga, named PR24 , was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Protothecablaschkeae SAG2064 . However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics. The inability to grow in Sabouraud broth at pH 4.0 and the different cellular fatty acid composition clearly distinguished PR24 from the reference strain of P. blaschkeae. The combination of genotypic and phenotypic data indicates that strain PR24 represents a subspecies of P. blaschkeae, for which the name Prototheca blaschkeae subsp. brasiliensis subsp. nov. is proposed. The respective type strain is PR24 (=DSM 103592 =IHEM 26958 ).
[Mh] Termos MeSH primário: Bovinos/microbiologia
Leite/microbiologia
Filogenia
Prototheca/classificação
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Brasil
DNA de Algas/genética
Ácidos Graxos/química
Feminino
Mastite Bovina
Prototheca/genética
Prototheca/isolamento & purificação
RNA Ribossômico 18S/genética
RNA Ribossômico 28S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (Fatty Acids); 0 (RNA, Ribosomal, 18S); 0 (RNA, Ribosomal, 28S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002209


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[PMID]:28054184
[Au] Autor:Hou LL; Liu F; Zang X; Zhang X; He B; Ding Y; Song X; Xiao D; Wang H
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, 266003, People's Republic of China.
[Ti] Título:Cloning and transcription analysis of the nitrate reductase gene from Haematococcus pluvialis.
[So] Source:Biotechnol Lett;39(4):589-597, 2017 Apr.
[Is] ISSN:1573-6776
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To optimize the cultivation media for the growth rate of Haematococcus pluvialis and to study the transcription regulation of the algal nitrate reductase (NR), a key enzyme for nitrogen metabolism. RESULTS: The NR gene from H. pluvialis hd7 consists of 5636 nucleotides, including 14 introns. The cDNA ORF is 2718 bp, encoding a 905 aa protein with three conserved domains. The NR amino acids of H. pluvialis hd7 are hydrophilic and have similarity of 72% compared to that of Dunaliella. NR transcription increased with an increase of nitrate concentration from 0.4 to 1 g/l. A deficiency of nitrogen increased NR transcription significantly. The transcription level of NR increased at phosphorus concentrations from 0.08 to 0.2 g/l, with a maximum at 0.08 g/l. The optimum parameters of medium component for transcription of NR and growth of H. pluvialis were 0.3 g NaNO /l, 0.045 g KH PO /l and 1.08 g sodium acetate/l. CONCLUSIONS: This study provides a better understanding of nitrate regulation in H. pluvialis.
[Mh] Termos MeSH primário: Proteínas de Algas/genética
Clorófitas/enzimologia
Expressão Gênica
Nitrato Redutase/genética
Nitratos/metabolismo
[Mh] Termos MeSH secundário: Ácido Acético/metabolismo
Sequência de Aminoácidos
Técnicas de Cultura de Células
Clorófitas/genética
DNA de Algas/genética
Nitrogênio/metabolismo
Fósforo/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Algal Proteins); 0 (DNA, Algal); 0 (Nitrates); 27YLU75U4W (Phosphorus); EC 1.7.99.4 (Nitrate Reductase); N762921K75 (Nitrogen); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1007/s10529-016-2283-0


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[PMID]:28038977
[Au] Autor:Cui C; Li Y; Liu Y; Li X; Luo S; Zhang Z; Wu R; Liang G; Sun J; Peng J; Tian P
[Ad] Endereço:National Engineering Science Research & Development Center of Algae and Sea Cucumbers of China, Provincial Key Laboratory of Genetic Improvement & Efficient Culture of Marine Algae of Shandong, Shandong Oriental Ocean Sci-tech Co. Ltd., 264003 Yantai, Shandong, PR China. Electronic address:
[Ti] Título:Determination of genetic diversity among Saccharina germplasm using ISSR and RAPD markers.
[So] Source:C R Biol;340(2):76-86, 2017 Feb.
[Is] ISSN:1768-3238
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Various species of genus Saccharina are economically important brown macroalgae cultivated in China. The genetic background of the conserved Saccharina germplasm was not clear. In this report, DNA-based molecular markers such as inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) were used to assess the genetic diversity and phylogenetic relationships among 48 Saccharina germplasms. A total of 50 ISSR and 50 RAPD primers were tested, of which only 33 polymorphic primers (17 ISSR and 16 RAPD) had an amplified clear and reproducible profile, and could be used. Seventeen ISSR primers yielded a total of 262 bands, of which 256 were polymorphic, and 15.06 polymorphic bands per primer were amplified from 48 kelp gametophytes. Sixteen RAPD primers produced 355 bands, of which 352 were polymorphic, and 22 polymorphic bands per primer were observed across 48 individuals. The simple matching coefficient of ISSR, RAPD and pooled ISSR and RAPD dendrograms ranged from 0.568 to 0.885, 0.670 to 0.873, and 0.667 to 0.862, revealing high genetic diversity. Based on the unweighted pair group method with the arithmetic averaging algorithm (UPGMA) cluster analysis and the principal components analysis (PCA) of ISSR data, the 48 gametophytes were divided into three main groups. The Mantel test revealed a similar polymorphism distribution pattern between ISSR and RAPD markers, the correlation coefficient r was 0.62, and the results indicated that both ISSR and RAPD markers were effective to assess the selected gametophytes, while matrix correlation of the ISSR marker system (r=0.78) was better than that of the RAPD marker system (r=0.64). Genetic analysis data from this study were helpful in understanding the genetic relationships among the selected 17 kelp varieties (or lines) and provided guidance for molecular-assisted selection for parental gametophytes of hybrid kelp breeding.
[Mh] Termos MeSH primário: Variação Genética/genética
Kelp/genética
Repetições de Microssatélites/genética
Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
[Mh] Termos MeSH secundário: Algoritmos
DNA de Algas/genética
Marcadores Genéticos
Células Germinativas Vegetais
Filogenia
Polimorfismo Genético/genética
Análise de Componente Principal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (Genetic Markers)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170101
[St] Status:MEDLINE


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[PMID]:28004878
[Au] Autor:Sullivan BK; Robinson KL; Trevathan-Tackett SM; Lilje ES; Gleason FH; Lilje O
[Ad] Endereço:School of Biosciences, Victorian Marine Science Consortium, University of Melbourne, Queenscliff, Vic., 3225, Australia.
[Ti] Título:The First Isolation and Characterisation of the Protist Labyrinthula sp. in Southeastern Australia.
[So] Source:J Eukaryot Microbiol;64(4):504-513, 2017 Jul.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a result of anthropogenic influences and global climate change, emerging infectious marine diseases are thought to be increasingly more common and more severe than in the past. The aim of our investigation was to confirm the presence of Labyrinthula, the aetiological agent of the seagrass wasting disease, in Southeastern Australia and provide the first isolation and characterisation of this protist, in Australia. Colonies and individual cells were positively identified as Labyrinthula using published descriptions, diagrams, and photographs. Their identity was then confirmed using DNA barcoding of a region of the 18S rRNA gene. Species level identification of isolates was not possible as the taxonomy of the Labyrinthula is still poorly resolved. Still, a diversity of Labyrinthula was isolated from small sections of the southeast coast of Australia. The isolates were grouped into three haplotypes that are biogeographically restricted. These haplotypes are closely related to previously identified saprotrophic clades. The study highlights the need for further investigation into the global distribution of Labyrinthula, including phylogenetic pathogenicity and analysis of host-parasite interactions in response to stressors. Given the results of our analyses, it is prudent to continue research into disease and epidemic agents to better prepare researchers for potential future outbreaks.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico/métodos
Magnoliopsida/parasitologia
Estramenópilas/classificação
Estramenópilas/isolamento & purificação
[Mh] Termos MeSH secundário: Austrália
Mudança Climática
DNA de Algas/genética
DNA Ribossômico/genética
Haplótipos
Interações Hospedeiro-Parasita
Filogenia
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
Estramenópilas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12387


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[PMID]:27988936
[Au] Autor:Sherwood AR; Dittbern MN; Johnston ET; Conklin KY
[Ad] Endereço:Department of Botany, University of Hawai'i, 3190 Maile Way, Honolulu, Hawai'i, 96822, USA.
[Ti] Título:A metabarcoding comparison of windward and leeward airborne algal diversity across the Ko'olau mountain range on the island of O'ahu, Hawai'i .
[So] Source:J Phycol;53(2):437-445, 2017 Apr.
[Is] ISSN:1529-8817
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Airborne algae from sites on the windward (n = 3) and leeward (n = 3) sides of the Ko'olau Mountain range of O'ahu, Hawai'i, were sampled for a 16 d period during January and February 2015 using passive collection devices and were characterized using Illumina MiSeq sequencing of the universal plastid amplicon marker. Amplicons were assigned to 3,023 operational taxonomic units (OTUs), which included 1,189 cyanobacteria, 1,009 heterotrophic bacteria, and 304 Eukaryota (of which 284 were algae and land plants). Analyses demonstrated substantially more OTUs at windward than leeward O'ahu sites during the sampling period. Removal of nonalgal OTUs revealed a greater number of algal reads recovered from windward (839,853) than leeward sites (355,387), with the majority of these being cyanobacteria. The 1,234 total algal OTUs included cyanobacteria, diatoms, cryptophytes, brown algae, chlorophyte green algae, and charophyte green algae. A total of 208 algal OTUs were identified from leeward side samplers (including OTUs in common among samplers) and 1,995 algal OTUs were identified from windward samplers. Barcoding analyses of the most abundant algal OTUs indicated that very few were shared between the windward and leeward sides of the Ko'olau Mountains, highlighting the localized scale at which these airborne algae communities differ. Back trajectories of air masses arriving on O'ahu during the sampling period were calculated using the NOAA HY-SPLIT model and suggested that the sampling period was composed of three large-scale meteorological events, indicating a diversity of potential sources of airborne algae outside of the Hawaiian Islands.
[Mh] Termos MeSH primário: Clorófitas/genética
[Mh] Termos MeSH secundário: Clorófitas/classificação
Cianobactérias/classificação
Cianobactérias/genética
DNA de Algas/genética
Ecossistema
Hawaii
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161219
[St] Status:MEDLINE
[do] DOI:10.1111/jpy.12502


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[PMID]:27917584
[Au] Autor:Hamilton TL; Havig J
[Ad] Endereço:Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA.
[Ti] Título:Primary productivity of snow algae communities on stratovolcanoes of the Pacific Northwest.
[So] Source:Geobiology;15(2):280-295, 2017 Mar.
[Is] ISSN:1472-4669
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The majority of geomicrobiological research conducted on glacial systems to date has focused on glaciers that override primarily carbonate or granitic bedrock types, with little known of the processes that support microbial life in glacial systems overriding volcanic terrains (e.g., basalt or andesite). To better constrain the role of the supraglacial ecosystems in the carbon and nitrogen cycles, to gain insight into microbiome composition and function in alpine glacial systems overriding volcanic terrains, and to constrain potential elemental sequestration or release through weathering processes associated with snow algae communities, we examined the microbial community structure and primary productivity of snow algae communities on stratovolcanoes in the Cascade Range of the Pacific Northwest. Here, we present the first published values for carbon fixation rates of snow algae communities on glaciers in the Pacific Northwest. We observed varying levels of light-dependent carbon fixation on supraglacial and periglacial snowfields at Mt. Hood, Mt. Adams, and North Sister. Recovery of abundant 18S rRNA transcripts affiliated with photoautotrophs and 16S rRNA transcripts affiliated with heterotrophic bacteria is consistent with previous studies indicating the majority of primary productivity on snow and ice can be attributed to photoautotrophs. In contrast to previous observations of glacial ecosystems, our geochemical, isotopic, and microcosm data suggest these assemblages are not limited by phosphorus or fixed nitrogen availability. Furthermore, our data indicate these snow algae communities actively sequester Fe, Mn, and P leached from minerals sourced from the local rocks. Our observations of light-dependent primary productivity on snow are consistent with similar studies in polar ecosystems; however, our data may suggest that DIC may be a limiting nutrient in contrast to phosphorus or fixed nitrogen as has been observed in other glacial ecosystems. Our data underscore the need for similar studies on glacier surfaces and seasonal snowfields to better constrain the role of local bedrock and nutrient delivery on carbon fixation and biogeochemical cycling in these ecosystems.
[Mh] Termos MeSH primário: Biota
Ciclo do Carbono
Microalgas/classificação
Microalgas/metabolismo
Neve/microbiologia
[Mh] Termos MeSH secundário: Bactérias/classificação
Análise por Conglomerados
DNA de Algas/química
DNA de Algas/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Noroeste dos Estados Unidos
Filogenia
RNA Ribossômico 16S/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE
[do] DOI:10.1111/gbi.12219


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[PMID]:27888475
[Au] Autor:Dawidziuk A; Popiel D; Luboinska M; Grzebyk M; Wisniewski M; Koczyk G
[Ad] Endereço:Functional Evolution of Biological Systems Team, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479, Poznan, Poland. adaw@igr.poznan.pl.
[Ti] Título:Assessing contamination of microalgal astaxanthin producer Haematococcus cultures with high-resolution melting curve analysis.
[So] Source:J Appl Genet;58(2):277-285, 2017 May.
[Is] ISSN:2190-3883
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Due to its superior antioxidant capabilities and higher activity than other carotenoids, astaxanthin is used widely in the nutraceutical and medicine industries. The most prolific natural producer of astaxanthin is the unicellular green microalga Haematococcus pluvialis. The correct identification of any contaminants in H. pluvialis cultures is both essential and nontrivial for several reasons. Firstly, while it is possible to distinguish the main microalgal contaminant Coelastrella sp. (in H. pluvialis cultures), in practice, it is frequently a daunting and error-prone task for personnel without extensive experience in the microscopic identification of algal species. Secondly, the undetected contaminants may decrease or stop production of astaxanthin. Lastly, the presence of other contaminants such as fungi can eventually infect and destroy the whole algae collection. In this study, high-resolution melting (HRM) analysis was developed to detect microalgal and fungal contamination. The developed diagnostic procedure allowed to distinguish pure H. pluvialis samples from cultures contaminated with low amounts (1.25 ng/ml) of microalgal DNA and fungal DNA (2.5 ng/ml). Such discrimination is not possible with the use of microscopy observations and allows fast and efficient collection testing.
[Mh] Termos MeSH primário: Clorófitas/metabolismo
DNA de Algas/isolamento & purificação
DNA Fúngico/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Bases
Contaminação por DNA
Limite de Detecção
Microalgas/genética
Xantofilas/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Algal); 0 (DNA, Fungal); 0 (Xanthophylls); 8XPW32PR7I (astaxanthine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161127
[St] Status:MEDLINE
[do] DOI:10.1007/s13353-016-0378-x


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[PMID]:27816910
[Au] Autor:Dorrell RG; Klinger CM; Newby RJ; Butterfield ER; Richardson E; Dacks JB; Howe CJ; Nisbet ER; Bowler C
[Ad] Endereço:Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR 8197, INSERM U1024, Paris, France.
[Ti] Título:Progressive and Biased Divergent Evolution Underpins the Origin and Diversification of Peridinin Dinoflagellate Plastids.
[So] Source:Mol Biol Evol;34(2):361-379, 2017 Feb 01.
[Is] ISSN:1537-1719
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dinoflagellates are algae of tremendous importance to ecosystems and to public health. The cell biology and genome organization of dinoflagellate species is highly unusual. For example, the plastid genomes of peridinin-containing dinoflagellates encode only a minimal number of genes arranged on small elements termed "minicircles". Previous studies of peridinin plastid genes have found evidence for divergent sequence evolution, including extensive substitutions, novel insertions and deletions, and use of alternative translation initiation codons. Understanding the extent of this divergent evolution has been hampered by the lack of characterized peridinin plastid sequences. We have identified over 300 previously unannotated peridinin plastid mRNAs from published transcriptome projects, vastly increasing the number of sequences available. Using these data, we have produced a well-resolved phylogeny of peridinin plastid lineages, which uncovers several novel relationships within the dinoflagellates. This enables us to define changes to plastid sequences that occurred early in dinoflagellate evolution, and that have contributed to the subsequent diversification of individual dinoflagellate clades. We find that the origin of the peridinin dinoflagellates was specifically accompanied by elevations both in the overall number of substitutions that occurred on plastid sequences, and in the Ka/Ks ratio associated with plastid sequences, consistent with changes in selective pressure. These substitutions, alongside other changes, have accumulated progressively in individual peridinin plastid lineages. Throughout our entire dataset, we identify a persistent bias toward non-synonymous substitutions occurring on sequences encoding photosystem I subunits and stromal regions of peridinin plastid proteins, which may have underpinned the evolution of this unusual organelle.
[Mh] Termos MeSH primário: Carotenoides/genética
Dinoflagelados/genética
Plastídeos/genética
[Mh] Termos MeSH secundário: Evolução Biológica
Códon
DNA de Algas/genética
Evolução Molecular
Variação Genética
Genomas de Plastídeos
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (DNA, Algal); 33281-81-1 (peridinin); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE
[do] DOI:10.1093/molbev/msw235



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