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  1 / 2203 MEDLINE  
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[PMID]:29251473
[Au] Autor:Dong L; Meng Y; Wang J; Sun G
[Ti] Título:Effects of Transgenic Bt+CpTI cotton on the abundance and diversity of rhizosphere ammonia oxidizing bacteria and archaea.
[So] Source:J Environ Biol;37(5):881-8, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Genetically modified crops (GMCs) hold great promise for improving agricultural output, but at the same time present challenges in terms of environmental safety assessment. Ammonia oxidizers, including ammonia oxidizing bacteria (AOB) and archaea (AOA), are very important functional microbial groups in nitrogen cycle. The abundance and diversity of AOA and AOB in the rhizosphere of genetically modified cotton (SGK321) and non-GM cotton (SY321) across growth stages were investigated using real time quantitative PCR (qPCR) and terminal restriction fragment length polymorphism (T-RFLP). Results showed that cotton genotype had a significant effect on the change in abundance of AOA and AOB, as indicated by amoA copy number. Variations in AOB abundance in rhizosphere of SY321 differed from those in SGK321. The number of AOB in the rhizosphere of SY321 fluctuated considerably: It dramatically decreased from 1.2?106 copies g-1 dry soil to 3?105 copies g-1 dry soil during the flowering stage and then increased to 1.1?106 copies g-1 and 1.5?106 copies g-1 at the belling and boll opening stages, respectively. However, abundance of AOB in the rhizosphere of SGK321 was relatively stable during all the stages of growth. The effect of SGK321 and SY321 on AOA number was quite similar to that of AOB: AOA abundance in SGK321 increased smoothly from 1.0 ?105 copies g-1 dry soil to 1.4?106 copies g-1 dry soil during growth, but that in SY321 fluctuated. Correspondence analysis (CA), canonical CA (CCA), and partial CCA (pCCA) of T-RFLP profiles of AOA and AOB showed that AOB community changed across growth stages in both cotton genotypes, and cotton genotype was the most important factor affecting the AOA community. In conclusion, the current findings indicated no adverse effect of GM cotton on functional microorganisms.
[Mh] Termos MeSH primário: Amônia/metabolismo
Archaea/metabolismo
Bactérias/metabolismo
Gossypium/genética
Microbiologia do Solo
[Mh] Termos MeSH secundário: Archaea/classificação
Bactérias/classificação
DNA Arqueal/genética
DNA Bacteriano/genética
Oxirredução
Raízes de Plantas
Plantas Geneticamente Modificadas
Solo
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (DNA, Bacterial); 0 (Soil); 7664-41-7 (Ammonia)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  2 / 2203 MEDLINE  
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[PMID]:28463103
[Au] Autor:Le TN; Wagner A; Albers SV
[Ad] Endereço:Molecular Biology of Archaea, Institute of Biology II - Microbiology, University of Freiburg, Schaenzlestrasse 1, 79104 Freiburg, Germany.
[Ti] Título:A conserved hexanucleotide motif is important in UV-inducible promoters in Sulfolobus acidocaldarius.
[So] Source:Microbiology;163(5):778-788, 2017 May.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Upon DNA damage, Sulfolobales exhibit a global gene regulatory response resulting in the expression of DNA transfer and repair proteins and the repression of the cell division machinery. Because the archaeal DNA damage response is still poorly understood, we investigated the promoters of the highly induced ups operon. Ups pili are involved in cellular aggregation and DNA exchange between cells. With LacS reporter gene assays we identified a conserved, non-palindromic hexanucleotide motif upstream of the ups core promoter elements to be essential for promoter activity. Substitution of this cis regulatory motif in the ups promoters resulted in abolishment of cellular aggregation and reduced DNA transfer. By screening the Sulfolobus acidocaldarius genome we identified a total of 214 genes harbouring the hexanucleotide motif in their respective promoter regions. Many of these genes were previously found to be regulated upon UV light treatment. Given the fact that the identified motif is conserved among S. acidocaldarius and Sulfolobus tokodaii promoters, we speculate that a common regulatory mechanism is present in these two species in response to DNA-damaging conditions.
[Mh] Termos MeSH primário: DNA Arqueal/efeitos da radiação
Regulação da Expressão Gênica em Archaea/efeitos da radiação
Motivos de Nucleotídeos/genética
Regiões Promotoras Genéticas/efeitos da radiação
Sulfolobus acidocaldarius/genética
Raios Ultravioleta
[Mh] Termos MeSH secundário: Dano ao DNA/genética
Dano ao DNA/efeitos da radiação
Reparo do DNA/genética
Reparo do DNA/efeitos da radiação
DNA Arqueal/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.000455


  3 / 2203 MEDLINE  
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[PMID]:29388536
[Au] Autor:Zhao B; Hu Q; Guo X; Liao Z; Sarmiento F; Mesbah NM; Yan Y; Li J; Wiegel J
[Ad] Endereço:1​Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
[Ti] Título:Natronolimnobius aegyptiacus sp. nov., an extremely halophilic alkalithermophilic archaeon isolated from the athalassohaline Wadi An Natrun, Egypt.
[So] Source:Int J Syst Evol Microbiol;68(2):498-506, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An obligately aerobic extremely halophilic alkalithermophilic archaeon, strain JW/NM-HA 15 , was isolated from the sediments of Wadi An Natrun in Egypt. Phylogenetic analysis based on 16S rRNA and rpoB' gene sequences indicated that it belongs to the family Natrialbaceae of the order Natrialbales. The closest relatives were Natronolimnobius baerhuensis IHC-005 and Natronolimnobius innermongolicus N-1311 (95.3 and 94.5 % 16S rRNA gene sequence similarity, respectively). Genome relatedness between strain JW/NM-HA 15 and its neighbours was evaluated using average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity with the values of 75.7-85.0, 18.1-20.0, and 70.2-71.0%, respectively. Cells were obligately aerobic, rod-shaped, non-motile, Gram-stain-negative and chemo-organotrophic. The strain grew in the presence of 2.57 M to saturating Na (optimum 3.25-4.60 M Na ), at pH 7.5-10.5 (optimum pH 9.0-9.5), and at 30-56 °C (optimum 52 °C). The major polar lipids consisted of phosphatidylglycerol, methylated phosphatidylglycerolphosphate and two phospholipids. The complete genome size of strain JW/NM-HA 15 is approximately 3.93 Mb, with a DNA G+C content of 64.1 mol%. On the basis of phylogenetic features, genomic relatedness, phenotypic and chemotaxonomic data, strain JW/NM-HA 15 was thus considered to represent a novel species within the genus Natronolimnobius, for which the name Natronolimnobius aegyptiacus sp. nov. is proposed. The type strain is JW/NM-HA 15 (=ATCC BAA-2088 =DSM 23470 ).
[Mh] Termos MeSH primário: Euryarchaeota/classificação
Filogenia
[Mh] Termos MeSH secundário: Composição de Bases
DNA Arqueal/genética
Egito
Euryarchaeota/genética
Euryarchaeota/isolamento & purificação
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002524


  4 / 2203 MEDLINE  
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[PMID]:29227219
[Au] Autor:Gibtan A; Song HS; Kim JY; Kim YB; Park N; Park K; Lee SJ; Kwon J; Roh SW; Lee HS
[Ad] Endereço:1​Department of Food Biotechnology, College of Medical and Life Sciences, Silla University, Busan 46958, Republic of Korea.
[Ti] Título:Halorubrum aethiopicum sp. nov., an extremely halophilic archaeon isolated from commercial rock salt.
[So] Source:Int J Syst Evol Microbiol;68(1):416-422, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel extremely halophilic archaeon, designated SAH-A6 , was isolated from a sample of commercial rock salt in Ethiopia. Cells of SAH-A6 were aerobic and pleomorphic. The strain was able to grow at concentrations of 15-30 % (w/v) NaCl (optimum 20-25 % NaCl), at pH 6.0-9.0 (optimum pH 7.0) and in a temperature range of 30-55 °C (optimum 37-45 °C). Mg was not required for growth of SAH-A6 cells. On the basis of 16S rRNA gene sequence analysis, strain SAH-A6 was closely related to Halorubrum halodurans Cb34 (99.1 %), Halorubrum rubrum YC87 (98.9 %), Halorubrum aquaticum EN-2 (98.7 %), Halorubrum cibi JCM 15757 (98.4 %), Halorubrum luteum CGSA15 (97.3 %), Halorubrum lipolyticum 9-3 (97.1 %), Halorubrum tibetense 8W8 (97.1 %), Halorubrum kocurii JCM 1478 (97.1 %), Halorubrum halophilum B8 (97.0 %) and Halorubrum persicum C49 (97.0 %). Phylogenetic analysis based on the rpoB' gene sequences showed that strain SAH-A6 was closely related to Hrr. halodurans Cb34 (99.7 %), Hrr. aquaticum JCM 14031 (99.3 %) and other members of the genus Halorubrum (<99.0 %). The DNA G+C content of the strain was 68.0 mol%. DNA-DNA hybridization between strain SAH-A6 and the most closely related members of the genus Halorubrum were below 55 %, suggesting that the new isolate constitutes a different genospecies. On the bases of chemotaxonomic, phenotypic and genotypic data, strain SAH-A6 (=KCCM 43215 =JCM 31519 ) represents a novel species of the genus Halorubrum, for which the name Halorubrumaethiopicum sp. nov. is proposed.
[Mh] Termos MeSH primário: Halorubrum/classificação
Filogenia
Cloreto de Sódio
[Mh] Termos MeSH secundário: Composição de Bases
DNA Arqueal/genética
Etiópia
Halorubrum/genética
Halorubrum/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (RNA, Ribosomal, 16S); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002525


  5 / 2203 MEDLINE  
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[PMID]:27771305
[Au] Autor:Walter ME; Ortiz A; Sondgeroth C; Sindt NM; Duszenko N; Catlett JL; Zhou Y; Valloppilly S; Anderson C; Fernando S; Buan NR
[Ad] Endereço:Redox Biology Center, Department of Biochemistry, University of Nebraska-Lincoln, N200 Beadle Center, Lincoln, NE 68588-0664, United States.
[Ti] Título:High-throughput mutation, selection, and phenotype screening of mutant methanogenic archaea.
[So] Source:J Microbiol Methods;131:113-121, 2016 12.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bacterial and archaeal genomes can contain 30% or more hypothetical genes with no predicted function. Phylogenetically deep-branching microbes, such as methane-producing archaea (methanogens), contain up to 50% genes with unknown function. In order to formulate hypotheses about the function of hypothetical gene functions in the strict anaerobe, Methanosarcina acetivorans, we have developed high-throughput anaerobic techniques to UV mutagenize, screen, and select for mutant strains in 96-well plates. Using these approaches we have isolated 10 mutant strains that exhibit a variety of physiological changes including increased or decreased growth rate relative to the parent strain when cells use methanol and/or acetate as carbon and energy sources. This method provides an avenue for the first step in identifying new gene functions: associating a genetic mutation with a reproducible phenotype. Mutations in bona fide methanogenesis genes such as corrinoid methyltransferases and proton-translocating F H :methanophenazine oxidoreductase (Fpo) were also generated, opening the door to in vivo functional complementation experiments. Irradiation-based mutagenesis such as from ultraviolet (UV) light, combined with modern genome sequencing, is a useful procedure to discern systems-level gene function in prokaryote taxa that can be axenically cultured but which may be resistant to chemical mutagens.
[Mh] Termos MeSH primário: Archaea/genética
Archaea/isolamento & purificação
Archaea/efeitos da radiação
Ensaios de Triagem em Larga Escala/métodos
Fenótipo
Mutação Puntual/efeitos da radiação
Raios Ultravioleta
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Archaea/metabolismo
DNA Arqueal/genética
DNA Arqueal/efeitos da radiação
Genes Arqueais
Metano/metabolismo
Metanol/metabolismo
Methanosarcina/genética
Methanosarcina/crescimento & desenvolvimento
Methanosarcina/efeitos da radiação
Metiltransferases/genética
Viabilidade Microbiana/efeitos da radiação
Mutagênese/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (DNA, Archaeal); EC 2.1.1.- (Methyltransferases); OP0UW79H66 (Methane); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  6 / 2203 MEDLINE  
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[PMID]:29211756
[Au] Autor:Kropp HM; Betz K; Wirth J; Diederichs K; Marx A
[Ad] Endereço:Konstanz Research School Chemical Biology, University of Konstanz, Baden-Württemberg, Konstanz, Germany.
[Ti] Título:Crystal structures of ternary complexes of archaeal B-family DNA polymerases.
[So] Source:PLoS One;12(12):e0188005, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Archaeal B-family polymerases drive biotechnology by accepting a wide substrate range of chemically modified nucleotides. By now no structural data for archaeal B-family DNA polymerases in a closed, ternary complex are available, which would be the basis for developing next generation nucleotides. We present the ternary crystal structures of KOD and 9°N DNA polymerases complexed with DNA and the incoming dATP. The structures reveal a third metal ion in the active site, which was so far only observed for the eukaryotic B-family DNA polymerase δ and no other B-family DNA polymerase. The structures reveal a wide inner channel and numerous interactions with the template strand that provide space for modifications within the enzyme and may account for the high processivity, respectively. The crystal structures provide insights into the superiority over other DNA polymerases concerning the acceptance of modified nucleotides.
[Mh] Termos MeSH primário: Archaea/enzimologia
DNA Polimerase Dirigida por DNA/química
[Mh] Termos MeSH secundário: Domínio Catalítico
Cristalografia por Raios X
DNA Arqueal/química
Modelos Moleculares
Conformação de Ácido Nucleico
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); EC 2.7.7.7 (DNA-Directed DNA Polymerase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188005


  7 / 2203 MEDLINE  
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[PMID]:27773688
[Au] Autor:Nakae S; Hijikata A; Tsuji T; Yonezawa K; Kouyama KI; Mayanagi K; Ishino S; Ishino Y; Shirai T
[Ad] Endereço:Department of Bioscience, Nagahama Institute of Bio-Science and Technology, Tamura 1266, Nagahama, Shiga 526-0829, Japan.
[Ti] Título:Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease.
[So] Source:Structure;24(11):1960-1971, 2016 11 01.
[Is] ISSN:1878-4186
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Archaeal NucS nuclease was thought to degrade the single-stranded region of branched DNA, which contains flapped and splayed DNA. However, recent findings indicated that EndoMS, the orthologous enzyme of NucS, specifically cleaves double-stranded DNA (dsDNA) containing mismatched bases. In this study, we determined the structure of the EndoMS-DNA complex. The complex structure of the EndoMS dimer with dsDNA unexpectedly revealed that the mismatched bases were flipped out into binding sites, and the overall architecture most resembled that of restriction enzymes. The structure of the apo form was similar to the reported structure of Pyrococcus abyssi NucS, indicating that movement of the C-terminal domain from the resting state was required for activity. In addition, a model of the EndoMS-PCNA-DNA complex was preliminarily verified with electron microscopy. The structures strongly support the idea that EndoMS acts in a mismatch repair pathway.
[Mh] Termos MeSH primário: DNA de Cadeia Simples/metabolismo
Endodesoxirribonucleases/química
Endodesoxirribonucleases/metabolismo
Pyrococcus abyssi/enzimologia
[Mh] Termos MeSH secundário: Proteínas Arqueais/química
Proteínas Arqueais/metabolismo
Sítios de Ligação
Reparo de Erro de Pareamento de DNA
DNA Arqueal/química
DNA Arqueal/metabolismo
DNA de Cadeia Simples/química
Microscopia Eletrônica
Modelos Moleculares
Ligação Proteica
Conformação Proteica
Pyrococcus abyssi/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (DNA, Archaeal); 0 (DNA, Single-Stranded); EC 3.1.- (Endodeoxyribonucleases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171230
[Lr] Data última revisão:
171230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE


  8 / 2203 MEDLINE  
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[PMID]:28911114
[Au] Autor:Liu T; Liu Z; Ye Q; Pan S; Wang X; Li Y; Peng W; Liang Y; She Q; Peng N
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
[Ti] Título:Coupling transcriptional activation of CRISPR-Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus.
[So] Source:Nucleic Acids Res;45(15):8978-8992, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CRISPR-Cas system provides the adaptive immunity against invading genetic elements in prokaryotes. Recently, we demonstrated that Csa3a regulator mediates spacer acquisition in Sulfolobus islandicus by activating the expression of Type I-A adaptation cas genes. However, links between the activation of spacer adaptation and CRISPR transcription/processing, and the requirement for DNA repair genes during spacer acquisition remained poorly understood. Here, we demonstrated that de novo spacer acquisition required Csa1, Cas1, Cas2 and Cas4 proteins of the Sulfolobus Type I-A system. Disruption of genes implicated in crRNA maturation or DNA interference led to a significant accumulation of acquired spacers, mainly derived from host genomic DNA. Transcriptome and proteome analyses showed that Csa3a activated expression of adaptation cas genes, CRISPR RNAs, and DNA repair genes, including herA helicase, nurA nuclease and DNA polymerase II genes. Importantly, Csa3a specifically bound the promoters of the above DNA repair genes, suggesting that they were directly activated by Csa3a for adaptation. The Csa3a regulator also specifically bound to the leader sequence to activate CRISPR transcription in vivo. Our data indicated that the Csa3a regulator couples transcriptional activation of the CRISPR-Cas system and DNA repair genes for spacer adaptation and efficient interference of invading genetic elements.
[Mh] Termos MeSH primário: Proteínas Arqueais/genética
Sistemas CRISPR-Cas
Reparo do DNA
DNA Arqueal/genética
Regulação da Expressão Gênica em Archaea
Sulfolobus/genética
Ativação Transcricional
[Mh] Termos MeSH secundário: Proteínas Arqueais/imunologia
Sequência de Bases
Proteínas Associadas a CRISPR/genética
Proteínas Associadas a CRISPR/imunologia
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
DNA Helicases/genética
DNA Helicases/imunologia
DNA Polimerase II/genética
DNA Polimerase II/imunologia
DNA Arqueal/imunologia
Endodesoxirribonucleases/genética
Endodesoxirribonucleases/imunologia
Chaperonas Moleculares/genética
Chaperonas Moleculares/imunologia
Regiões Promotoras Genéticas
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Sulfolobus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (CRISPR-Associated Proteins); 0 (DNA, Archaeal); 0 (Molecular Chaperones); EC 2.7.7.- (DNA Polymerase II); EC 3.1.- (Endodeoxyribonucleases); EC 3.6.4.- (DNA Helicases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx612


  9 / 2203 MEDLINE  
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[PMID]:28905703
[Au] Autor:Yin XQ; Liu BB; Chu X; Salam N; Li X; Yang ZW; Zhang Y; Xiao M; Li WJ
[Ad] Endereço:1​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
[Ti] Título:Saliphagus infecundisoli gen. nov., sp. nov., an extremely halophilic archaeon isolated from a saline soil.
[So] Source:Int J Syst Evol Microbiol;67(10):4154-4160, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two extremely halophilic archaea, strains YIM 93745 and YIM 93707, were isolated from a saline soil sample collected from Loulan, China. Cells of the two strains were coccus, non-motile and Gram-stain negative. The strains were aerobic and grew at 25-50 °C (optimum, 37 °C), in the presence of 5-35 % (w/v) NaCl (optimum, 20 %), 0.01-0.1 M Mg (optimum, 0.03 M) and pH 6.0-8.5 (optimum, 7.0-7.5). Cells lysed in distilled water and with 0-5 % NaCl. Major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glycosyl diether and two unidentified glycolipids. Phylogenetic analysis based on the 16S rRNA sequence revealed that the two strains were most closely related to Halovivax cerinus IC35 (95.1 and 95.2 % sequence similarities, respectively). The two strains, however, shared highest rpoB' gene sequence identities with Natrinema pellirubrum JCM 10476 (87.8 and 87.7 % respectively). Phylogenetic trees based on 16S rRNA and rpoB' gene sequences demonstrated a robust clade of the two strains with members of related genera of the family Natrialbaceae. The DNA G+C contents of the two strains were 64.6 and 64.4 mol%, respectively. DNA-DNA relatedness values between them were 95±2 %. Phenotypic, chemotaxonomic characteristics and phylogenetic properties suggested that the two strains YIM 93745 and YIM 93707 represent a novel species in a new genus within the family Natrialbaceae, for which the name Saliphagus infecundisoli gen. nov., sp. nov. is proposed. The type strain is YIM 93745 (=KCTC 4228 =CGMCC 1.15824 ).
[Mh] Termos MeSH primário: Halobacteriaceae/classificação
Filogenia
Salinidade
Microbiologia do Solo
[Mh] Termos MeSH secundário: China
DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/química
Halobacteriaceae/genética
Halobacteriaceae/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002270


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[PMID]:28901903
[Au] Autor:Lü ZZ; Li Y; Zhou Y; Cui HL; Li ZR
[Ad] Endereço:1​School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
[Ti] Título:Halobacterium litoreum sp. nov., isolated from a marine solar saltern.
[So] Source:Int J Syst Evol Microbiol;67(10):4095-4099, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Halophilic archaeal strain ZS-54-S2 was isolated from Zhoushan marine solar saltern, China. Cells were rod-shaped, Gram-stain-negative and formed red-pigmented colonies on an agar plate. Strain ZS-54-S2 was able to grow at 20-50 °C (optimum 35 °C), at 1.7-4.8 M NaCl (optimum 3.9 M), at 0.005-1.0 M MgCl2 (optimum 0.05 M) and at pH 5.0-9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two glycolipids, which were chromatographically identical to sulfated galactosyl mannosyl galactofuranosyl glucosyl diether and galactosyl mannosyl glucosyl diether, and an unidentified glycolipid, which was chromatographically identical to one detected in Halobacterium salinarum ATCC 33171 . The 16S rRNA gene and rpoB' gene of strain ZS-54-S2 were phylogenetically related to the corresponding genes of Halobacterium noricense JCM 15102 (97.5 % and 90.6 % relatedness, respectively), Halobacterium jilantaiense CGMCC 1.5337 (96.9 and 91.2 %), Halobacterium rubrum CGMCC 1.12575 (96.8 and 90.3 %) and Halobacterium salinarum CGMCC 1.1958 (96.5 and 88.4 %). The DNA G+C content of strain ZS-54-S2 was 66.7 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-54-S2 (=CGMCC 1.12562 =JCM 30038 ) represents a new species of Halobacterium, for which the name Halobacteriumlitoreum sp. nov. is proposed.
[Mh] Termos MeSH primário: Halobacterium/classificação
Filogenia
Salinidade
Microbiologia da Água
[Mh] Termos MeSH secundário: Composição de Bases
China
DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/análise
Halobacterium/genética
Halobacterium/isolamento & purificação
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002259



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