Base de dados : MEDLINE
Pesquisa : D13.444.308.315 [Categoria DeCS]
Referências encontradas : 3654 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 366 ir para página                         

  1 / 3654 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28456662
[Au] Autor:Kinkar L; Laurimäe T; Sharbatkhori M; Mirhendi H; Kia EB; Ponce-Gordo F; Andresiuk V; Simsek S; Lavikainen A; Irshadullah M; Umhang G; Oudni-M'rad M; Acosta-Jamett G; Rehbein S; Saarma U
[Ad] Endereço:Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 50410 Tartu, Estonia.
[Ti] Título:New mitogenome and nuclear evidence on the phylogeny and taxonomy of the highly zoonotic tapeworm Echinococcus granulosus sensu stricto.
[So] Source:Infect Genet Evol;52:52-58, 2017 Aug.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cystic echinococcosis, a zoonotic disease caused by Echinococcus granulosus sensu lato (s. l.), is a significant global public health concern. Echinococcus granulosus s. l. is currently divided into numerous genotypes (G1-G8 and G10) of which G1-G3 are the most frequently implicated genotypes in human infections. Although it has been suggested that G1-G3 could be regarded as a distinct species E. granulosus sensu stricto (s. s.), the evidence to support this is inconclusive. Most importantly, data from nuclear DNA that provide means to investigate the exchange of genetic material between G1-G3 is lacking as none of the published nuclear DNA studies have explicitly included G2 or G3. Moreover, the commonly used relatively short mtDNA sequences, including the complete cox1 gene, have not allowed unequivocal differentiation of genotypes G1-G3. Therefore, significantly longer mtDNA sequences are required to distinguish these genotypes with confidence. The main aim of this study was to evaluate the phylogenetic relations and taxonomy of genotypes G1-G3 using sequences of nearly complete mitogenomes (11,443bp) and three nuclear loci (2984bp). A total of 23 G1-G3 samples were analysed, originating from 5 intermediate host species in 10 countries. The mtDNA data demonstrate that genotypes G1 and G3 are distinct mitochondrial genotypes (separated by 37 mutations), whereas G2 is not a separate genotype or even a monophyletic cluster, but belongs to G3. Nuclear data revealed no genetic separation of G1 and G3, suggesting that these genotypes form a single species due to ongoing gene flow. We conclude that: (a) in the taxonomic sense, genotypes G1 and G3 can be treated as a single species E. granulosus s. s.; (b) genotypes G1 and G3 should be regarded as distinct genotypes only in the context of mitochondrial data; (c) we recommend excluding G2 from the genotype list.
[Mh] Termos MeSH primário: Núcleo Celular/genética
DNA de Helmintos/genética
Echinococcus granulosus/classificação
Mitocôndrias/genética
[Mh] Termos MeSH secundário: África do Norte
Animais
Ásia
Echinococcus granulosus/genética
Echinococcus granulosus/isolamento & purificação
Echinococcus granulosus/metabolismo
Europa (Continente)
Genoma Mitocondrial
Genótipo
Seres Humanos
Filogenia
Filogeografia
América do Sul
Zoonoses/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  2 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29191179
[Au] Autor:Fierst JL; Murdock DA
[Ad] Endereço:Department of Biological Sciences, University of Alabama, Tuscaloosa, 35487, AL, USA. jlfierst@ua.edu.
[Ti] Título:Decontaminating eukaryotic genome assemblies with machine learning.
[So] Source:BMC Bioinformatics;18(1):533, 2017 Dec 01.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: High-throughput sequencing has made it theoretically possible to obtain high-quality de novo assembled genome sequences but in practice DNA extracts are often contaminated with sequences from other organisms. Currently, there are few existing methods for rigorously decontaminating eukaryotic assemblies. Those that do exist filter sequences based on nucleotide similarity to contaminants and risk eliminating sequences from the target organism. RESULTS: We introduce a novel application of an established machine learning method, a decision tree, that can rigorously classify sequences. The major strength of the decision tree is that it can take any measured feature as input and does not require a priori identification of significant descriptors. We use the decision tree to classify de novo assembled sequences and compare the method to published protocols. CONCLUSIONS: A decision tree performs better than existing methods when classifying sequences in eukaryotic de novo assemblies. It is efficient, readily implemented, and accurately identifies target and contaminant sequences. Importantly, a decision tree can be used to classify sequences according to measured descriptors and has potentially many uses in distilling biological datasets.
[Mh] Termos MeSH primário: Caenorhabditis/genética
Aprendizado de Máquina
[Mh] Termos MeSH secundário: Animais
Composição de Bases
DNA de Helmintos/química
DNA de Helmintos/isolamento & purificação
DNA de Helmintos/metabolismo
Bases de Dados Genéticas
Genoma
Sequenciamento de Nucleotídeos em Larga Escala
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1941-0


  3 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28982192
[Au] Autor:Lee MR; Canales-Aguirre CB; Nuñez D; Pérez K; Hernández CE; Brante A
[Ad] Endereço:Centro i~mar, Universidad de Los Lagos, Puerto Montt, Chile.
[Ti] Título:The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal.
[So] Source:PLoS One;12(10):e0186140, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The diversity of free-living nematodes in the beaches of two Antarctic islands, King George and Deception islands was investigated. We used morphological and molecular (LSU, and two fragments of SSU sequences) approaches to evaluate 236 nematodes. Specimens were assigned to at least genera using morphology and were assessed for the presence of cryptic speciation. The following genera were identified: Halomonhystera, Litoditis, Enoploides, Chromadorita, Theristus, Oncholaimus, Viscosia, Gammanema, Bathylaimus, Choanolaimus, and Paracanthonchus; along with specimens from the families Anticomidae and Linhomoeidae. Cryptic speciation was identified within the genera Halomonhystera and Litoditis. All of the cryptic species identified live sympatrically. The two cryptic species of Halomonhystera exhibited no significant morphological differences. However, Litoditis species 2 was significantly larger than Litoditis species 1. The utility of molecular data in confirming the identifications of some of the morphologically more challenging families of nematodes was demonstrated. In terms of which molecular sequences to use for the identification of free-living nematodes, the SSU sequences were more variable than the LSU sequences, and thus provided more resolution in the identification of cryptic speciation. Finally, despite the considerable amount of time and effort required to put together genetic and morphological data, the resulting advance in our understanding of diversity and ecology of free-living marine nematodes, makes that effort worthwhile.
[Mh] Termos MeSH primário: Nematoides/classificação
[Mh] Termos MeSH secundário: Animais
Regiões Antárticas
Teorema de Bayes
DNA de Helmintos/isolamento & purificação
Nematoides/genética
Filogenia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186140


  4 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28822206
[Au] Autor:Umar S; Shinkafi SH; Hudu SA; Neela V; Suresh K; Nordin SA; Malina O
[Ad] Endereço:Department of Microbiology and Parasitology, Usmanu Danfodiyo University Teaching Hospital Sokoto, 80002 Sokoto State, Nigeria
[Ti] Título:Prevalence and molecular characterisation of Schistosoma haematobium among primary school children in Kebbi State, Nigeria
[So] Source:Ann Parasitol;63(2):133-139, 2017.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Schistosomiasis is the major source of morbidity in Sub-Saharan Africa and Asia. It is estimated that 207 million people are infected, of which 97% are in Africa. The aim of this study was the determining of prevalence as well as the phylogeny of S. haematobium among school children in Argungu Emirate, Kebbi State Nigeria. A total of 325 urine samples was collected from school children between 7 to 14 years. S. heamatobium eggs was examined under dissecting microscope and DNA was extracted from urine sample and COX1 gene was amplified by nested PCR. The PCR products were purified, sequenced and analysed. This study showed a prevalence of 32.09%, with male pupils having the highest prevalence. S. haematobium infections in children who fetch water in the river have 24 times higher risk of being infected while those who bath in the river have 158 times higher risk of being infected. Our sequences were phylogenetically related to S. haematobium isolate U82266 from Kenya and consistence with the predominant species in Africa. This was the first S. haematobium and S. mansoni co-infection reported in Nigeria. S. haematobium infection is prevalent among school age and significantly associated with water contact.
[Mh] Termos MeSH primário: Schistosoma haematobium/genética
Esquistossomose Urinária/epidemiologia
[Mh] Termos MeSH secundário: Animais
Criança
DNA de Helmintos/genética
Seres Humanos
Nigéria/epidemiologia
Filogenia
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.17420/ap6302.97


  5 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28803904
[Au] Autor:Gao JF; Liu GH; Duan H; Gao Y; Zhang Y; Chang QC; Fang M; Wang CR
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China; Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province 161006, PR China. Electronic address: gaojunfeng_2005
[Ti] Título:Complete mitochondrial genomes of Triodontophorus serratus and Triodontophorus nipponicus, and their comparison with Triodontophorus brevicauda.
[So] Source:Exp Parasitol;181:88-93, 2017 Oct.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Triodontophorus serratus and Triodontophorus nipponicus are two of the most common nematodes inhabiting in the large intestine of horse. In the present study, the complete mitochondrial (mt) genome sequences of T. serratus and T. nipponicus have been determined. The mt genomes of T. serratus and T. nipponicus are circular molecules with 13,794 bp and 13,701 bp in size, respectively. These circular mt genomes encode 36 genes, including 12 protein-coding genes, two rRNA genes and 22 tRNA genes. All of these genes are transcribed in the same direction and gene arrangements are consistent with that of gene arrangement 3 (GA3-type). T. serratus and T. nipponicus had two non-coding regions, but T. brevicauda had three. Phylogenetic relationships were reconstructed using concatenated amino acid sequences of the 12 protein-coding genes with three methods, indicating that three species of Triodontophorus clustered together with strong statistical support. However, the genera of Strongylus and Triodontophorus belonged to Strongylinae do not cluster together, and Triodontophorus is more closely related to Cylicocyclus insigne, Cylicocyclus nassatus, Cylicostephanus goldi (Cyathostominae) than to Strongylus. The findings from the present study provide useful genetic markers for studying the molecular ecology, systematics, and population genetics of Triodontophorus in equine.
[Mh] Termos MeSH primário: Genoma Mitocondrial
Doenças dos Cavalos/parasitologia
Infecções por Strongylida/veterinária
Strongyloidea/genética
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Ceco/parasitologia
Colo/parasitologia
DNA de Helmintos/química
DNA Mitocondrial/química
Ordem dos Genes
Genoma Mitocondrial/genética
Cavalos
Filogenia
Infecções por Strongylida/parasitologia
Strongyloidea/classificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Mitochondrial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE


  6 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28793306
[Au] Autor:Jaleta TG; Zhou S; Bemm FM; Schär F; Khieu V; Muth S; Odermatt P; Lok JB; Streit A
[Ad] Endereço:Department of Evolutionary Biology, Max-Planck-Institute for Developmental Biology, Tübingen, Germany.
[Ti] Título:Different but overlapping populations of Strongyloides stercoralis in dogs and humans-Dogs as a possible source for zoonotic strongyloidiasis.
[So] Source:PLoS Negl Trop Dis;11(8):e0005752, 2017 Aug.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Strongyloidiasis is a much-neglected soil born helminthiasis caused by the nematode Strongyloides stercoralis. Human derived S. stercoralis can be maintained in dogs in the laboratory and this parasite has been reported to also occur in dogs in the wild. Some authors have considered strongyloidiasis a zoonotic disease while others have argued that the two hosts carry host specialized populations of S. stercoralis and that dogs play a minor role, if any, as a reservoir for zoonotic S. stercoralis infections of humans. We isolated S. stercoralis from humans and their dogs in rural villages in northern Cambodia, a region with a high incidence of strongyloidiasis, and compared the worms derived from these two host species using nuclear and mitochondrial DNA sequence polymorphisms. We found that in dogs there exist two populations of S. stercoralis, which are clearly separated from each other genetically based on the nuclear 18S rDNA, the mitochondrial cox1 locus and whole genome sequence. One population, to which the majority of the worms belong, appears to be restricted to dogs. The other population is indistinguishable from the population of S. stercoralis isolated from humans. Consistent with earlier studies, we found multiple sequence variants of the hypervariable region I of the 18 S rDNA in S. stercoralis from humans. However, comparison of mitochondrial sequences and whole genome analysis suggest that these different 18S variants do not represent multiple genetically isolated subpopulations among the worms isolated from humans. We also investigated the mode of reproduction of the free-living generations of laboratory and wild isolates of S. stercoralis. Contrary to earlier literature on S. stercoralis but similar to other species of Strongyloides, we found clear evidence of sexual reproduction. Overall, our results show that dogs carry two populations, possibly different species of Strongyloides. One population appears to be dog specific but the other one is shared with humans. This argues for the strong potential of dogs as reservoirs for zoonotic transmission of S. stercoralis to humans and suggests that in order to reduce the exposure of humans to infective S. stercoralis larvae, dogs should be treated for the infection along with their owners.
[Mh] Termos MeSH primário: Doenças do Cão/parasitologia
Polimorfismo Genético
Strongyloides stercoralis/classificação
Strongyloides stercoralis/isolamento & purificação
Estrongiloidíase/parasitologia
Estrongiloidíase/veterinária
Zoonoses/parasitologia
[Mh] Termos MeSH secundário: Animais
Camboja/epidemiologia
Análise por Conglomerados
DNA de Helmintos/química
DNA de Helmintos/genética
DNA Mitocondrial/química
DNA Mitocondrial/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Reservatórios de Doenças
Doenças do Cão/epidemiologia
Doenças do Cão/transmissão
Cães
Complexo IV da Cadeia de Transporte de Elétrons/genética
Genótipo
Seres Humanos
Epidemiologia Molecular
Filogenia
RNA Ribossômico 18S/genética
População Rural
Análise de Sequência de DNA
Strongyloides stercoralis/genética
Estrongiloidíase/epidemiologia
Estrongiloidíase/transmissão
Zoonoses/epidemiologia
Zoonoses/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Mitochondrial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 18S); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005752


  7 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28757123
[Au] Autor:Umair S; Bouchet CLG; Knight JS; Pernthaner A; Simpson HV
[Ad] Endereço:AgResearch Ltd, Private Bag 11-008, Palmerston North, New Zealand. Electronic address: saleh.umair@agresearch.co.nz.
[Ti] Título:Molecular and biochemical characterisation and recognition by the immune host of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of the abomasal nematode parasite Teladorsagia circumcincta.
[So] Source:Exp Parasitol;181:40-46, 2017 Oct.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A 1023 bp full length cDNA encoding Teladorsagia circumcincta GAPDH (TeciGAPDH) was cloned, expressed in Escherichia coli and the recombinant protein purified and its kinetic properties determined. A phylogenetic tree was constructed using helminth GAPDH sequences. The predicted protein consisted of 341 amino acids and was present as a single band of about 38 kDa on SDS-PAGE. Multiple alignments of the protein sequence of TeciGAPDH with homologues from other helminths showed that the greatest similarity (93%) to the GAPDH of Haemonchus contortus and Dictyocaulus viviparus, 82-86% similarity to the other nematode sequences and 68-71% similarity to cestode and trematode enzymes. Substrate binding sites and conserved regions were identified and were completely conserved in other homologues. At 25 °C, the optimum pH for TeciGAPDH activity was pH 8, the V was 1052 ± 23 nmol min mg protein and the apparent K for the substrate glyceraldehyde-3-phosphate was 0.02 ± 0.01 mM (both mean ± SD, n = 2). Antibodies in both serum and saliva from field-immune, but not nematode-naïve, sheep recognised recombinant TeciGAPDH in enzyme-linked immunosorbent assays. The recognition of the recombinant protein by antibodies generated by exposure of sheep to native GAPDH indicates similar antigenicity of the two proteins.
[Mh] Termos MeSH primário: Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/imunologia
Trichostrongyloidea/enzimologia
[Mh] Termos MeSH secundário: Abomaso/parasitologia
Sequência de Aminoácidos
Animais
Sequência de Bases
DNA Complementar/química
DNA Complementar/isolamento & purificação
DNA de Helmintos/química
DNA de Helmintos/isolamento & purificação
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/química
Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/genética
Concentração de Íons de Hidrogênio
Filogenia
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Alinhamento de Sequência
Ovinos
Doenças dos Ovinos/parasitologia
Trichostrongyloidea/classificação
Trichostrongyloidea/genética
Trichostrongyloidea/imunologia
Tricostrongiloidíase/parasitologia
Tricostrongiloidíase/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (DNA, Helminth); 0 (Recombinant Proteins); EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


  8 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28733132
[Au] Autor:Guivier E; Lippens C; Faivre B; Sorci G
[Ad] Endereço:Biogéosciences, CNRS UMR 6282, Université de Bourgogne Franche-Comté, 6 Bd Gabriel, 21000 Dijon, France; Physiopathologie des dyslipidémies, INSERM UMR 866, Université de Bourgogne Franche-Comté, 21000 Dijon, France. Electronic address: em.guivier@gmail.com.
[Ti] Título:Plastic and micro-evolutionary responses of a nematode to the host immune environment.
[So] Source:Exp Parasitol;181:14-22, 2017 Oct.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parasitic organisms have to cope with the defences deployed by their hosts and this can be achieved adopting immune evasion strategies or optimal life history traits according to the prevailing pattern of immune-mediated mortality. Parasites often encounter variable immune environments both within and between hosts, promoting the evolution of plastic strategies instead of fixed responses. Here, we explored the plasticity and micro-evolutionary responses of immunomodulatory mechanisms and life history traits to the immune environment provided by the host, using the parasitic nematode Heligmosomoides polygyrus. To test if the parasite responds plastically to the immune environment, we stimulated the systemic inflammatory response of mice and we assessed i) the expression of two genes with candidate immunomodulatory functions (Hp-Tgh2 and Hp-CPI); ii) changes in the number of eggs shed in the faeces. To test if the immune environment induces a micro-evolutionary response in the parasite, we maintained the nematode in mice whose inflammatory response was up- or down-regulated during four generations. We found that H. polygyrus plastically responded to a sudden rise of pro-inflammatory cytokines, up-regulating the expression of two candidate genes involved in the process of immune modulation, and enhancing egg output. At the micro-evolutionary level, parasites maintained in hosts experiencing different levels of inflammation did not have differential expression of Hp-Tgh2 and Hp-CPI genes when infecting unmanipulated, control, mice. However, parasites maintained in mice with an up-regulated inflammation shed more eggs compared to the control line. Overall, our study shows that H. polygyrus can plastically adjust the expression of immunomodulatory genes and life history traits, and responds to selection exerted by the host immune system.
[Mh] Termos MeSH primário: Nematospiroides dubius/imunologia
Infecções por Strongylida/imunologia
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Citocinas/sangue
DNA Complementar/química
DNA Complementar/isolamento & purificação
DNA de Helmintos/química
DNA de Helmintos/isolamento & purificação
Fezes/parasitologia
Feminino
Expressão Gênica
Imunomodulação/genética
Modelos Lineares
Camundongos
Camundongos Endogâmicos BALB C
Nematospiroides dubius/genética
Contagem de Ovos de Parasitas
RNA de Helmintos/isolamento & purificação
RNA Mensageiro/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Inoculações Seriadas
Infecções por Strongylida/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (DNA, Complementary); 0 (DNA, Helminth); 0 (RNA, Helminth); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170723
[St] Status:MEDLINE


  9 / 3654 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28692673
[Au] Autor:McLennan MR; Hasegawa H; Bardi M; Huffman MA
[Ad] Endereço:Anthropology Centre for Conservation, Environment and Development, Oxford Brookes University, Oxford, United Kingdom.
[Ti] Título:Gastrointestinal parasite infections and self-medication in wild chimpanzees surviving in degraded forest fragments within an agricultural landscape mosaic in Uganda.
[So] Source:PLoS One;12(7):e0180431, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Monitoring health in wild great apes is integral to their conservation and is especially important where they share habitats with humans, given the potential for zoonotic pathogen exchange. We studied the intestinal parasites of wild chimpanzees (Pan troglodytes schweinfurthii) inhabiting degraded forest fragments amid farmland and villages in Bulindi, Uganda. We first identified protozoan and helminth parasites infecting this population. Sixteen taxa were demonstrated microscopically (9 protozoa, 5 nematodes, 1 cestode, and 1 trematode). DNA sequence analysis enabled more precise identification of larval nematodes (e.g. Oesophagostomum stephanostomum, O. bifurcum, Strongyloides fuelleborni, Necator sp. Type II) and tapeworm proglottids (genus Bertiella). To better understand the ecology of infections, we used multidimensional scaling analysis to reveal general patterns of association among parasites, climate, and whole leaf swallowing-a prevalent self-medicative behaviour at Bulindi linked to control of nodular worms (Oesophagostomum spp.). Prevalence of parasites varied with climate in diverse ways. For example, Oesophagostomum sp. was detected in faeces at higher frequencies with increasing rainfall but was most clearly associated with periods of low temperature. Certain parasites occurred together within chimpanzee hosts more or less frequently than expected by chance. For example, the commensal ciliate Troglodytella abrassarti was negatively associated with Balantidium coli and Oesophagostomum sp., possibly because the latter taxa make the large intestine less suitable for T. abrassarti. Whole leaves in faeces showed independent associations with the prevalence of Oesophagostomum sp., Strongyloides sp., and hookworm by microscopic examination, and with egestion of adult O. stephanostomum by macroscopic inspection. All parasites identified to species or genus have been reported in wild chimpanzees inhabiting less-disturbed environments than Bulindi. Nevertheless, several disease-causing taxa infecting these chimpanzees are potentially transmissible between apes and humans (e.g. rhabditoid and strongyle nematodes), underscoring the importance of identifying and reducing risks of pathogen exchange in shared landscapes.
[Mh] Termos MeSH primário: Animais Selvagens/parasitologia
Doenças dos Símios Antropoides/parasitologia
Conservação dos Recursos Naturais
Florestas
Enteropatias Parasitárias/parasitologia
Enteropatias Parasitárias/veterinária
Pan troglodytes/parasitologia
Parasitos/fisiologia
[Mh] Termos MeSH secundário: Agricultura
Animais
Doenças dos Símios Antropoides/epidemiologia
Sequência de Bases
Biodiversidade
DNA de Helmintos/genética
Fezes/parasitologia
Helmintos/genética
Umidade
Enteropatias Parasitárias/epidemiologia
Folhas de Planta/fisiologia
Prevalência
Chuvas
Estações do Ano
Automedicação
Análise de Sequência de DNA
Especificidade da Espécie
Temperatura Ambiente
Fatores de Tempo
Uganda/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180431


  10 / 3654 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28692668
[Au] Autor:Papaiakovou M; Pilotte N; Grant JR; Traub RJ; Llewellyn S; McCarthy JS; Krolewiecki AJ; Cimino R; Mejia R; Williams SA
[Ad] Endereço:Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America.
[Ti] Título:A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool.
[So] Source:PLoS Negl Trop Dis;11(7):e0005734, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Molecular-based surveys have indicated that Ancylostoma ceylanicum, a zoonotic hookworm, is likely the second most prevalent hookworm species infecting humans in Asia. Most current PCR-based diagnostic options for the detection of Ancylostoma species target the Internal Transcribed Spacer (ITS) regions of the ribosomal gene cluster. These regions possess a considerable degree of conservation among the species of this genus and this conservation can lead to the misidentification of infecting species or require additional labor for accurate species-level determination. We have developed a novel, real-time PCR-based assay for the sensitive and species-specific detection of A. ceylanicum that targets a non-coding, highly repetitive genomic DNA element. Comparative testing of this PCR assay with an assay that targets ITS sequences was conducted on field-collected samples from Argentina and Timor-Leste to provide further evidence of the sensitivity and species-specificity of this assay. METHODS/PRINCIPAL FINDINGS: A previously described platform for the design of primers/probe targeting non-coding highly repetitive regions was used for the development of this novel assay. The assay's limits of detection (sensitivity) and cross-reactivity with other soil-transmitted helminth species (specificity) were assessed with real-time PCR experiments. The assay was successfully used to identify infections caused by A. ceylanicum that were previously only identified to the genus level as Ancylostoma spp. when analyzed using other published primer-probe pairings. Further proof of sensitive, species-specific detection was provided using a published, semi-nested restriction fragment length polymorphism-PCR assay that differentiates between Ancylostoma species. CONCLUSIONS/SIGNIFICANCE: Due to the close proximity of people and domestic/wild animals in many regions of the world, the potential for zoonotic infections is substantial. Sensitive tools enabling the screening for different soil-transmitted helminth infections are essential to the success of mass deworming efforts and facilitate the appropriate interpretation of data. This study describes a novel, species-specific, real-time PCR-based assay for the detection of A. ceylanicum that will help to address the need for such tools in integrated STH deworming programs. TRIAL REGISTRATION: ANZCTR.org.au ACTRN12614000680662.
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Ancilostomíase/diagnóstico
Fezes/parasitologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Ancylostoma/classificação
Animais
Argentina
DNA de Helmintos/isolamento & purificação
Seres Humanos
Filogenia
Polimorfismo de Fragmento de Restrição
Ensaios Clínicos Controlados Aleatórios como Assunto
Especificidade da Espécie
Timor-Leste
Zoonoses/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005734



página 1 de 366 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde