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[PMID]:29408405
[Au] Autor:Wang H; Park BS; Lim WA; Ki JS
[Ad] Endereço:Department of Biotechnology, Sangmyung University, Seoul 03016, South Korea.
[Ti] Título:CpMCA, a novel metacaspase gene from the harmful dinoflagellate Cochlodinium polykrikoides and its expression during cell death.
[So] Source:Gene;651:70-78, 2018 Apr 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Metacaspases (MCAs) are cysteine proteases that share sequence homology with caspases, and may play roles in programmed cell death (PCD). In the present study, we identified a novel MCA gene (CpMCA) from the red tide dinoflagellate Cochlodinium polykrikoides, and examined its molecular characteristics and gene expression in response to algicide-induced cell death. CpMCA cDNA is 1164 bp in length, containing a dinoflagellate spliced leader sequence (dinoSL), an 879-bp open reading frame (ORF), which codes for a 293-aa protein, and a poly (A) tail. Multi-sequence comparison indicated that CpMCA belongs to type I MCA, but it has a different structure at the N-terminal. Phylogenetic analysis showed that C. polykrikoides may have acquired the MCA gene from bacteria by means of horizontal gene transfer (HGT). In addition, expressions of CpMCA significantly increased following exposure to the common algicides copper sulfate and oxidizing chlorine, which trigger cell death in dinoflagellates, suggesting that CpMCA may be involved in cell death.
[Mh] Termos MeSH primário: Caspases/genética
Dinoflagelados/genética
[Mh] Termos MeSH secundário: Morte Celular/efeitos dos fármacos
Morte Celular/genética
DNA Complementar
DNA de Protozoário
Dinoflagelados/efeitos dos fármacos
Dinoflagelados/enzimologia
Expressão Gênica
Transferência Genética Horizontal
Genes Bacterianos
Genes de Protozoários
Herbicidas/farmacologia
Filogenia
Análise de Sequência de DNA
Transcrição Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (DNA, Protozoan); 0 (Herbicides); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


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[PMID]:29320815
[Au] Autor:Matin S; Shahbazi G; Namin ST; Moradpour R; Feizi F; Piri-Dogahe H
[Ad] Endereço:Department of Internal Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
[Ti] Título:Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran.
[So] Source:Korean J Parasitol;55(6):607-611, 2017 Dec.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.
[Mh] Termos MeSH primário: Aborto Habitual/etiologia
Aborto Espontâneo/etiologia
Anticorpos Antiprotozoários/sangue
Reação em Cadeia da Polimerase/métodos
Complicações Parasitárias na Gravidez/diagnóstico
Testes Sorológicos/métodos
Toxoplasma/imunologia
Toxoplasmose/diagnóstico
Toxoplasmose/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biomarcadores/sangue
DNA de Protozoário/sangue
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Irã (Geográfico)/epidemiologia
Gravidez
Complicações Parasitárias na Gravidez/epidemiologia
Complicações Parasitárias na Gravidez/parasitologia
Toxoplasma/genética
Toxoplasmose/complicações
Toxoplasmose/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Biomarkers); 0 (DNA, Protozoan); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.6.607


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[PMID]:28453841
[Au] Autor:Requena-Méndez A; Goñi P; Rubio E; Pou D; Fumadó V; Lóbez S; Aldasoro E; Cabezos J; Valls ME; Treviño B; Martínez Montseny AF; Clavel A; Gascon J; Muñoz J
[Ad] Endereço:Barcelona Institute for Global Health, ISGlobal-CRESIB, Universitat de Barcelona, Barcelona , Spain.
[Ti] Título:The Use of Quinacrine in Nitroimidazole-resistant Giardia Duodenalis: An Old Drug for an Emerging Problem.
[So] Source:J Infect Dis;215(6):946-953, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: There is little evidence regarding the management of refractory giardiasis after treatment with nitroimidazoles. This study estimates the proportion of persistent giardiasis in 3 hospitals in Barcelona, describes associated risk factors and genotype, and evaluates the efficacy rate of quinacrine in those with persistent giardiasis. Methods: A clinical, prospective, observational study was conducted in patients with giardiasis treated with nitroimidazoles. Those with persistent giardiasis were provided quinacrine. Molecular characterization of Giardia isolates was performed by polymerase chain reaction amplification of a fragment of tpi and bg genes. Results: Seventy-seven patients were recruited and treated with nitroimidazoles, and in 14 of 71 (20%) of patients followed up, Giardia persisted. Refractory giardiasis was associated with malaise (P = .007) and anorexia (P = .02), with previous giardiasis (P = .03), and with previous antibiotic (P = .02) or antiparasitic(P = .04) use. Quinacrine had an effectiveness rate of 100% in refractory giardiasis (n = 13; 95% confidence interval = 75-100). Molecular characterization showed that 17 (25%) Giardia isolates belonged to assemblage A, and 31 (43%) belonged to assemblage B. In refractory giardiasis, assemblage A and B were found responsible in 4 and 6 cases, respectively. Conclusions: Almost 20% of patients presented persistent giardiasis, belonging to both assemblages A and B, after nitroimidazole. Short course of quinacrine was effective in treating refractory cases. Further controlled studies should evaluate its efficacy and safety.
[Mh] Termos MeSH primário: Giardia lamblia/genética
Giardíase/tratamento farmacológico
Nitroimidazóis/uso terapêutico
Quinacrina/uso terapêutico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Criança
Pré-Escolar
DNA de Protozoário/genética
Resistência a Medicamentos
Fezes/parasitologia
Feminino
Genótipo
Giardia lamblia/efeitos dos fármacos
Giardia lamblia/isolamento & purificação
Seres Humanos
Lactente
Recém-Nascido
Modelos Logísticos
Masculino
Análise Multivariada
Nitroimidazóis/efeitos adversos
Filogenia
Estudos Prospectivos
Quinacrina/efeitos adversos
Espanha
Viagem
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (Nitroimidazoles); H0C805XYDE (Quinacrine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix066


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[PMID]:28453188
[Au] Autor:Moritz ED; Tonnetti L; Hewins ME; Berardi VP; Dodd RY; Stramer SL
[Ad] Endereço:Scientific Affairs Department, American Red Cross, Gaithersburg, Maryland.
[Ti] Título:Description of 15 DNA-positive and antibody-negative "window-period" blood donations identified during prospective screening for Babesia microti.
[So] Source:Transfusion;57(7):1781-1786, 2017 07.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Blood donation screening detecting only antibodies fails to identify donors in the earliest stage of infection, before a detectable immunologic response, that is, the "window period" (WP). We present data on WP donations identified during prospective screening for Babesia microti, a transfusion-transmissible parasite of increasing concern in the United States. STUDY DESIGN AND METHODS: Blood donations collected in Connecticut, Massachusetts, Minnesota, and Wisconsin were screened using polymerase chain reaction (PCR) and arrayed fluorescence immunoassay (AFIA) to detect B. microti DNA and antibodies, respectively. Parasite loads were estimated using quantitative PCR. Red blood cell (RBC) samples were inoculated into hamsters to assess infectivity. Donors screening reactive were indefinitely deferred, tested by supplemental methods, and followed to assess DNA and antibody clearance. Demographic data from WP donors (i.e., those screening PCR positive and AFIA negative) were compared to data from other positive donors. RESULTS: Of 220,479 donations screened from June 2012 to August 2016, a total of 700 were positive, of which 15 (2% of positive donations or 1 per 14,699 screened donations) were confirmed WP donations. The median estimated parasite load in WP donations was 350 parasites/mL, no different than AFIA-positive and PCR-positive donors. Parasite loads in RBC samples from WP units ranged from 14 to 11,022 parasites/mL; RBC samples from three of 10 (30%) WP donations infected hamsters. The mean age of WP donors was 48 years (range, 17-75 years); three (20%) were female. WP donor demographics did not differ significantly from demographics of other donors. CONCLUSIONS: We report one per 15,000 B. microti WP infections in blood donors in endemic areas, demonstrating the importance of nucleic acid testing to mitigate the risk of transfusion-transmitted babesiosis.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Babesia microti/isolamento & purificação
Doadores de Sangue
DNA de Protozoário/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Babesia microti/genética
Babesia microti/imunologia
Feminino
Imunofluorescência
Seres Humanos
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Estudos Prospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (DNA, Protozoan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14103


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[PMID]:28964886
[Au] Autor:Zhuang Y; Clamp JC; Yi Z; Ji D
[Ad] Endereço:Ocean School, Yantai University, Yantai 264005, China.
[Ti] Título:Phylogeny of the families Zoothamniidae and Epistylididae (Protozoa: Ciliophora: Peritrichia) based on analyses of three rRNA-coding regions.
[So] Source:Mol Phylogenet Evol;118:99-107, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peritrichs are a major group of ciliates with worldwide distribution, and they play important roles in many habitats. Intrafamilial phylogeny of some peritrichs was investigated using information from three genes, which provided more robust interpretations than single-gene analyses. Sixty-seven new sequences including SSU rDNA, ITS1-5.8S-ITS2 and LSU rDNA were aligned with available sequences in GenBank to infer phylogenetic relationships within the families Zoothamniidae and Epistylididae. Results reveal the following relationships: (1) Epistylididae is polyphyletic, consisting of two clades that nest within the Zoothamniidae as part of the crown clade of peritrichs (order Vorticellida) and a third one that is part of the basal clade of peritrichs (order Opercularida); (2) Epistylis elongata falls within one of the clades of Zoothamnium rather than with congeners; (3) Zoothamnium is probably paraphyletic, consisting of three divergent clades, with the genera Myoschiston and Zoothamnopsis intermingled with species of Zoothamnium. The following evolutionary hypotheses can be inferred from these results: (1) the contractile stalk of Zoothamnium is plesiomorphic. (2) Myoschiston, Zoothamnopsis and clade II of Epistylididae are derived from the Zoothamnium morphotype by partial or incomplete development of the spasmoneme that forms the contractile center of the stalk around which the rigid cortex is secreted. (3) Clade I of the Epistylididae, which are primarily colonial forms that appear never to have evolved a spasmoneme of any sort, may represent the ancestral morphotype of peritrichs.
[Mh] Termos MeSH primário: Cilióforos/classificação
Fases de Leitura Aberta/genética
Filogenia
RNA Ribossômico/genética
[Mh] Termos MeSH secundário: Sequência de Bases
China
Cilióforos/genética
DNA de Protozoário/genética
DNA Ribossômico/genética
Geografia
Funções Verossimilhança
Subunidades Ribossômicas Menores/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171002
[St] Status:MEDLINE


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[PMID]:29342187
[Au] Autor:De Lucia S; Tsamesidis I; Pau MC; Kesely KR; Pantaleo A; Turrini F
[Ad] Endereço:Department of Oncology, University of Turin, Turin, Italy.
[Ti] Título:Induction of high tolerance to artemisinin by sub-lethal administration: A new in vitro model of P. falciparum.
[So] Source:PLoS One;13(1):e0191084, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Artemisinin resistance is a major threat to malaria control efforts. Resistance is characterized by an increase in the Plasmodium falciparum parasite clearance half-life following treatment with artemisinin-based combination therapies (ACTs) and an increase in the percentage of surviving parasites. The remarkably short blood half-life of artemisinin derivatives may contribute to drug-resistance, possibly through factors including sub-lethal plasma concentrations and inadequate exposure. Here we selected for a new strain of artemisinin resistant parasites, termed the artemisinin resistant strain 1 (ARS1), by treating P. falciparum Palo Alto (PA) cultures with sub-lethal concentrations of dihydroartemisinin (DHA). The resistance phenotype was maintained for over 1 year through monthly maintenance treatments with low doses of 2.5 nM DHA. There was a moderate increase in the DHA IC50 in ARS1 when compared with parental strain PA after 72 h of drug exposure (from 0.68 nM to 2 nM DHA). In addition, ARS1 survived treatment physiologically relevant DHA concentrations (700 nM) observed in patients. Furthermore, we confirmed a lack of cross-resistance against a panel of antimalarials commonly used as partner drugs in ACTs. Finally, ARS1 did not contain Pfk13 propeller domain mutations associated with ART resistance in the Greater Mekong Region. With a stable growth rate, ARS1 represents a valuable tool for the development of new antimalarial compounds and studies to further elucidate the mechanisms of ART resistance.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Antimaláricos/farmacologia
Artemisininas/farmacologia
Plasmodium falciparum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antimaláricos/administração & dosagem
Artemisininas/administração & dosagem
DNA de Protozoário/genética
DNA de Protozoário/isolamento & purificação
Relação Dose-Resposta a Droga
Resistência a Medicamentos
Genótipo
Concentração Inibidora 50
Plasmodium falciparum/genética
Plasmodium falciparum/fisiologia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimalarials); 0 (Artemisinins); 0 (DNA, Protozoan); 9RMU91N5K2 (artemisinine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191084


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[PMID]:29309786
[Au] Autor:Hijjawi N; Yang R; Hatmal M; Yassin Y; Mharib T; Mukbel R; Mahmoud SA; Al-Shudifat AE; Ryan U
[Ad] Endereço:Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, The Hashemite University, PO Box 150459, Zarqa, 13115, Jordan.
[Ti] Título:Comparison of ELISA, nested PCR and sequencing and a novel qPCR for detection of Giardia isolates from Jordan.
[So] Source:Exp Parasitol;185:23-28, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Little is known about the prevalence of Giardia duodenalis in human patients in Jordan and all previous studies have used direct microscopy, which lacks sensitivity. The present study developed a novel quantitative PCR (qPCR) assay at the ß-giardin (bg) locus and evaluated its use as a frontline test for the diagnosis of giardiasis in comparison with a commercially available ELISA using nested PCR and sequencing of the glutamate dehydrogenase (gdh) locus (gdh nPCR) as the gold standard. A total of 96 human faecal samples were collected from 96 patients suffering from diarrhoea from 5 regions of Jordan and were screened using the ELISA and qPCR. The analytical specificity of the bg qPCR assay revealed no cross-reactions with other genera and detected all the Giardia isolates tested. Analytical sensitivity was 1 Giardia cyst per µl of DNA extract. The overall prevalence of Giardia was 64.6%. The clinical sensitivity and specificity of the bg qPCR was 89.9% and 82.9% respectively compared to 76.5 and 68.0% for the ELISA. This study is the first to compare three different methods (ELISA, bg qPCR, nested PCR and sequencing at the gdh locus) to diagnose Jordanian patients suffering from giardiasis and to analyze their demographic data.
[Mh] Termos MeSH primário: Diarreia/parasitologia
Giardia lamblia/isolamento & purificação
Giardíase/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antígenos de Protozoários/análise
Criança
Pré-Escolar
Cólica/parasitologia
Proteínas do Citoesqueleto/genética
DNA de Protozoário/química
DNA de Protozoário/isolamento & purificação
Ensaio de Imunoadsorção Enzimática
Fezes/parasitologia
Feminino
Giardia lamblia/genética
Giardia lamblia/imunologia
Giardíase/epidemiologia
Glutamato Desidrogenase
Seres Humanos
Lactente
Jordânia/epidemiologia
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Prevalência
Proteínas de Protozoários/genética
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Vômito/parasitologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Cytoskeletal Proteins); 0 (DNA, Protozoan); 0 (Protozoan Proteins); 0 (giardin protein, Giardia lamblia); EC 1.4.1.2 (Glutamate Dehydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:29307564
[Au] Autor:Niikura M; Inoue SI; Fukutomi T; Yamagishi J; Asahi H; Kobayashi F
[Ad] Endereço:Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
[Ti] Título:Comparative genomics and proteomic analyses between lethal and nonlethal strains of Plasmodium berghei.
[So] Source:Exp Parasitol;185:1-9, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plasmodium berghei (Pb) XAT, a rodent malaria parasite, is an irradiation-attenuated variant derived from the lethal strain Pb NK65. Differences in genome sequence, protein structure and function between Pb XAT and Pb NK65 are currently unknown. In this study, to investigate genetic alterations in Pb XAT, we performed comparative genomics and proteomics analyses of nonlethal and lethal strains of Pb. We found mutations, such as a deletion mutation in rhoptry-associated protein (rap) 1, and deletion of rap2/3 and skeleton-binding protein 1 (sbp1), in Pb XAT. RAP1 is required for targeting of RAP2 to the rhoptries. However, the contribution of RAP2/3 to the lethality of Plasmodium is unclear. Therefore, we generated RAP1- and RAP2/3-deficient mutants of Pb ANKA, a reference strain of P. berghei. Furthermore, we investigated the effect of RAP1 and RAP2/3 deficiency on the outcome of infection. The parasitemia in mice infected with RAP1-deficient parasites was increased compared to that in control parasite-infected mice during the early phase of infection. However, mice infected with RAP1-deficient parasites survived longer than did control parasite-infected mice. Moreover, mice infected with RAP2/3-deficient parasites showed low levels of parasitemia and ultimately recovered from the infection The aim of this study was to investigate the effect of RAP2/3 expression on the outcome of infection with Pb XAT using a RAP2/3-expressing Pb XAT. Results showed that complementation of RAP2/3 expression in Pb XAT partially restored virulence. Our findings suggest that RAP1 and RAP2/3 contribute to virulence and a decrease in their expression explains the loss of virulence of the Pb XAT strain.
[Mh] Termos MeSH primário: Genômica
Malária/parasitologia
Plasmodium berghei/patogenicidade
Proteômica
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida
DNA de Protozoário/química
DNA de Protozoário/genética
Eritrócitos/parasitologia
Feminino
Malária/mortalidade
Camundongos
Camundongos Endogâmicos C57BL
Parasitemia/parasitologia
Plasmodium berghei/genética
Plasmodium berghei/metabolismo
Reação em Cadeia da Polimerase/métodos
Proteínas de Protozoários/genética
Transcrição Reversa
Deleção de Sequência
Organismos Livres de Patógenos Específicos
Espectrometria de Massas em Tandem
Virulência
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (Protozoan Proteins); 0 (rhoptry associated protein, Plasmodium); 145184-78-7 (rhoptry-associated antigen-2, Plasmodium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:29307563
[Au] Autor:Locatelli Dittrich R; Regidor-Cerrillo J; Ortega-Mora LM; Oliveira Koch M; Busch APB; Gonçalves KA; Cruz AA
[Ad] Endereço:Programa de Pós Graduação em Ciências Veterinárias, Setor de ciências Agrárias, Universidade Federal do Paraná, Rua dos Funcionários, 1540, CEP 80035-050, Curitiba, Paraná, Brazil. Electronic address: roslocdi@ufpr.br.
[Ti] Título:Isolation of Neospora caninum from kidney and brain of a bovine foetus and molecular characterization in Brazil.
[So] Source:Exp Parasitol;185:10-16, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine neosporosis has become a disease of international concern as it is among the main causes of abortion in cattle. Viable N. caninum has been isolated from brains of fetuses and neonatal calves, and there is no report of isolation of tachyzoites from kidney. Also, detailed information about the genetic diversity of N. caninum is scarce. N. caninum tachyzoites were isolated from the kidney and the brain of an aborted 4-month-old bovine foetus. The parasite was confirmed to be N. caninum by PCR. The tachyzoites of the new isolate, named BNC-PR4, were propagated in Vero cell cultures. Pathogenicity of the parasite was examined in BALB/c mice. Mice inoculated intraperitoneally with BNC-PR4 failed to yield clinical signs of disease and did not induce severe brain lesions, suggesting a bovine isolate with low virulence. The N. caninum-positive DNA sample was further analyzed by multilocus microsatellite (MS) genotyping for MS4, MS5, MS6A, MS6B, MS7, MS8, MS10, MS12, and MS21. Multilocus-microsatellite genotyping revealed a unique genetic profile that differed from previously reported isolates.
[Mh] Termos MeSH primário: Feto Abortado/parasitologia
Encéfalo/parasitologia
Doenças dos Bovinos/parasitologia
Coccidiose/veterinária
Rim/parasitologia
Neospora/isolamento & purificação
[Mh] Termos MeSH secundário: Aborto Animal/parasitologia
Animais
Encéfalo/embriologia
Brasil
Bovinos
Doenças dos Bovinos/embriologia
Cercopithecus aethiops
Coccidiose/embriologia
Coccidiose/parasitologia
DNA de Protozoário/química
DNA de Protozoário/isolamento & purificação
Indústria de Laticínios
Feminino
Rim/embriologia
Camundongos
Camundongos Endogâmicos BALB C
Neospora/genética
Neospora/patogenicidade
Células Vero
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:29320544
[Au] Autor:González C; León C; Paz A; López M; Molina G; Toro D; Ortiz M; Cordovez JM; Atencia MC; Aguilera G; Tovar C
[Ad] Endereço:Centro de Investigaciones en Microbiología y Parasitología Tropical, CIMPAT, Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
[Ti] Título:Diversity patterns, Leishmania DNA detection, and bloodmeal identification of Phlebotominae sand flies in villages in northern Colombia.
[So] Source:PLoS One;13(1):e0190686, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leishmaniases are neglected tropical diseases exhibiting complex transmission cycles due to the number of parasite species circulating, sand fly species acting as vectors and infected mammals, including humans, which are defined in the New World as accidental hosts. However, current transmission scenarios are changing, and the disease is no longer exclusively related to forested areas but urban transmission foci occur, involving some species of domestic animals as suspected reservoirs. The aim of this study was to determine the transmission cycles in urban environments by evaluating sand fly diversity, detection of Leishmania DNA, and bloodmeal sources through intra and peridomestic collections. The study was carried out in Colombia, in 13 municipalities of Cordoba department, implementing a methodology that could be further used for the evaluation of vector-borne diseases in villages or towns. Our sampling design included 24 houses randomly selected in each of 15 villages distributed in 13 municipalities, which were sampled in two seasons in 2015 and 2016. Sand flies were collected using CDC light traps placed in intra and peridomestic habitats. In addition to the morphological identification, molecular identification through DNA barcodes was also performed. A total of 19,743 sand flies were collected and 13,848 of them (10,268 females and 3,580 males) were used in molecular procedures. Circulation of two known parasite species-Leishmania infantum and Leishmania panamensis was confirmed. Blood source analyses showed that sand flies fed on humans, particularly in the case of the known L. infantum vector, P. evansi; further analyses are advised to evaluate the reservoirs involved in parasite transmission. Our sampling design allowed us to evaluate potential transmission cycles on a department scale, by defining suspected vector species, parasite species present in different municipalities and feeding habits.
[Mh] Termos MeSH primário: DNA de Protozoário/genética
Comportamento Alimentar
Variação Genética
Insetos Vetores/parasitologia
Leishmania/genética
Psychodidae/parasitologia
[Mh] Termos MeSH secundário: Animais
Colômbia
Psychodidae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190686



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