Base de dados : MEDLINE
Pesquisa : D13.444.600 [Categoria DeCS]
Referências encontradas : 530 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 53 ir para página                         

  1 / 530 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28000981
[Au] Autor:Defrancq E; Messaoudi S
[Ad] Endereço:Université Grenoble Alpes, CNRS, Département de Chimie Moléculaire, UMR 5250, B. P. 53, 38041, Grenoble Cedex 9, France.
[Ti] Título:Palladium-Mediated Labeling of Nucleic Acids.
[So] Source:Chembiochem;18(5):426-431, 2017 Mar 02.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:New applications of Pd-catalyzed coupling reactions (Suzuki-Miyaura, Sonogashira, and Stille-Migita coupling) for post-conjugation of nucleic acids have been developed recently. Breakthroughs in this area might now pave the way for the development of sophisticated DNA probes, which might be of great interest in chemical biology, nanotechnology, and bioanalysis, as well as in diagnostic domains.
[Mh] Termos MeSH primário: Sondas de Ácido Nucleico/química
Paládio/química
[Mh] Termos MeSH secundário: Catálise
Coloração e Rotulagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nucleic Acid Probes); 5TWQ1V240M (Palladium)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201600599


  2 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27865105
[Au] Autor:Liu H; Tian T; Zhang Y; Ding L; Yu J; Yan M
[Ad] Endereço:Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, China.
[Ti] Título:Sensitive and rapid detection of microRNAs using hairpin probes-mediated exponential isothermal amplification.
[So] Source:Biosens Bioelectron;89(Pt 2):710-714, 2017 Mar 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) play significant roles in a diverse range of biological progress and have been regarded as biomarkers and therapeutic targets in cancer treatment. Here, we develop a rapid and sensitive miRNA assay on the basis of exponential isothermal amplification in combination with the hairpin probes, which was designed for sensitive detection of miRNA. The binding of target miRNA with a linear DNA template initiates exponential isothermal amplification reaction (EXPAR) and generates the universal triggers which are complementary to the 3' protruding end of hairpin probe1(HP1). These triggers function not only as the primers to unfold the hairpin probes through catalysed hairpin assembly(CHA), generating distinct fluorescence signals, but also as the primer to initiate the next EXPAR. Moreover, CHA can release new triggers to initiate EXPAR or CHA. Thus this hairpin probes-mediated exponential isothermal amplification assay exhibits high sensitivity with a detection limit of 3.0×10 M. More importantly, the isothermal condition and simple fluorescence measurement would greatly promote the development of a fast, point-of-care detection system. It can be completed in an hour which can effectively avoid miRNA from degradation. This hairpin probe-based circular exponential amplification assay holds a great promise for further application in biomedical research and early clinical diagnosis.
[Mh] Termos MeSH primário: MicroRNAs/análise
Técnicas de Amplificação de Ácido Nucleico/métodos
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/métodos
Linhagem Celular Tumoral
Seres Humanos
Limite de Detecção
Conformação de Ácido Nucleico
Sondas de Ácido Nucleico/química
Sistemas Automatizados de Assistência Junto ao Leito
Espectrometria de Fluorescência/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN21 microRNA, human); 0 (MicroRNAs); 0 (Nucleic Acid Probes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE


  3 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27821510
[Au] Autor:Cantara WA; Hatterschide J; Wu W; Musier-Forsyth K
[Ad] Endereço:Department of Chemistry and Biochemistry, Center for Retrovirus Research, and Center for RNA Biology, The Ohio State University, Columbus, Ohio 43210, USA.
[Ti] Título:RiboCAT: a new capillary electrophoresis data analysis tool for nucleic acid probing.
[So] Source:RNA;23(2):240-249, 2017 Feb.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemical and enzymatic probing of RNA secondary structure and RNA/protein interactions provides the basis for understanding the functions of structured RNAs. However, the ability to rapidly perform such experiments using capillary electrophoresis has been hampered by relatively labor-intensive data analysis software. While these computationally robust programs have been shown to calculate residue-specific reactivities to a high degree of accuracy, they often require time-consuming manual intervention and lack the ability to be easily modified by users. To alleviate these issues, RiboCAT (Ribonucleic acid capillary-electrophoresis analysis tool) was developed as a user-friendly, Microsoft Excel-based tool that reduces the need for manual intervention, thereby significantly shortening the time required for data analysis. Features of this tool include (i) the use of an Excel platform, (ii) a method of intercapillary signal alignment using internal size standards, (iii) a peak-sharpening algorithm to more accurately identify peaks, and (iv) an open architecture allowing for simple user intervention. Furthermore, a complementary tool, RiboDOG (RiboCAT data output generator) was designed to facilitate the comparison of multiple data sets, highlighting potential inconsistencies and inaccuracies that may have occurred during analysis. Using these new tools, the secondary structure of the HIV-1 5' untranslated region (5'UTR) was determined using selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE), matching the results of previous work.
[Mh] Termos MeSH primário: Algoritmos
Eletroforese Capilar/estatística & dados numéricos
Sondas de Ácido Nucleico/análise
RNA Viral/análise
Software
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Acilação
Pareamento de Bases
Sequência de Bases
HIV-1/química
HIV-1/genética
Conformação de Ácido Nucleico
Sondas de Ácido Nucleico/química
RNA Viral/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Nucleic Acid Probes); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE
[do] DOI:10.1261/rna.058404.116


  4 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27184557
[Au] Autor:Ou X; Hong F; Zhang Z; Cheng Y; Zhao Z; Gao P; Lou X; Xia F; Wang S
[Ad] Endereço:Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China.
[Ti] Título:A highly sensitive and facile graphene oxide-based nucleic acid probe: Label-free detection of telomerase activity in cancer patient's urine using AIEgens.
[So] Source:Biosens Bioelectron;89(Pt 1):417-421, 2017 Mar 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Molecular beacon (MB)-based sensing platforms that consist of a fluorogen-quencher pair play an important role in medical and biological researches. However, the synthesis of both fluorogen and quencher in the nucleic acid probes will increase the burden of organic synthesis works and induce the difficulties for precisely controlling the relative distance between fluorogen and quencher, which may lead to false-positive and false-negative results. In this work, initially we report a single labeled MB (FAM-MB, with carboxyfluorescein as fluorogen and without quencher) thus simplifies MBs with the aid of graphene oxide (GO) to detect telomerase activity. To further simplify this structure, namely label-free strategy, we design a facile, sensitive and selective platform using a label-free beacon (AIE-MB, without fluorogen and quencher), based on aggregation-induced emission fluorogen (silole-R). Upon the addition of telomerase, AIE-MB induced comb-like DNA structure leads to high aggregation of silole-R and thus exhibits strong fluorescence emission. By exploitation of this, we can detect telomerase with superior sensitivity and demonstrate their applications in bladder cancer diagnosis. Compared to single-labeled FAM-MB based telomerase activity assay, the label-free AIE-MB induced method could perform the sensitive detection with high signal-to-background ratio.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Grafite/química
Sondas de Ácido Nucleico/química
Telomerase/urina
Neoplasias da Bexiga Urinária/urina
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Fluoresceínas/química
Corantes Fluorescentes/química
Células HeLa
Seres Humanos
Modelos Moleculares
Óxidos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluoresceins); 0 (Fluorescent Dyes); 0 (Nucleic Acid Probes); 0 (Oxides); 3301-79-9 (6-carboxyfluorescein); 7782-42-5 (Graphite); EC 2.7.7.49 (Telomerase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE


  5 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26944029
[Au] Autor:Robertson NM; Toscano AE; LaMantia VE; Hizir MS; Rana M; Balcioglu M; Sheng J; Yigit MV
[Ad] Endereço:Department of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY 12222, United States.
[Ti] Título:Unlocked Nucleic Acids for miRNA detection using two dimensional nano-graphene oxide.
[So] Source:Biosens Bioelectron;89(Pt 1):551-557, 2017 Mar 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study we have used Unlocked Nucleic Acids (UNAs) to discriminate a breast cancer oncomiR from two other miRNAs in the same RNA family using two-dimensional graphene oxide nanoassemblies. Fluorescently labeled single stranded probe strands and graphene oxide nanoassemblies have been used to detect miR-10b and discriminate it from miR-10a, which differs by only a single nucleotide (12th base from the 5' end), and miR-10c, which differs by only two nucleotides (12th and 16th bases from the 5' end). We have determined the discrimination efficacy and detection capacity of a DNA probe with two inserted UNA monomers (UNA ), and compared it to the DNA probe with two purposefully inserted mutations (DNA ) and full complementary sequence (DNA ). We have observed that UNA is 50 times more powerful than DNA in discriminating miR-10b from miR-10c while generating an equally high fluorescence signal. This fluorescence signal was then further enhanced with the use of the highly specific endonuclease dsDNase for an enzymatic amplification step. The results demonstrate that the underutilized UNAs have enormous potential for miRNA detection and offer remarkable discrimination efficacy over single and double mismatches.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Grafite/química
MicroRNAs/análise
Nanoestruturas/química
[Mh] Termos MeSH secundário: Sequência de Bases
Neoplasias da Mama/genética
Desoxirribonucleases/química
Feminino
Seres Humanos
MicroRNAs/genética
Nanoestruturas/ultraestrutura
Técnicas de Amplificação de Ácido Nucleico/métodos
Hibridização de Ácido Nucleico/métodos
Sondas de Ácido Nucleico/química
Sondas de Ácido Nucleico/genética
Óxidos/química
Espectrometria de Fluorescência/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN10 microRNA, human); 0 (MicroRNAs); 0 (Nucleic Acid Probes); 0 (Oxides); 7782-42-5 (Graphite); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160306
[St] Status:MEDLINE


  6 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27461906
[Au] Autor:Patkar N; Joshi S; Chaudhary S; Mascerhenas R; Doshi H; Tembhare P; Gujral S; Subramanian PG
[Ad] Endereço:Hematopathology Laboratory, Tata Memorial Centre, Mumbai, India.
[Ti] Título:Development of a cost-effective 'duplexed' real-time PCR assay for minimal residual disease monitoring of chronic myeloid leukemia using locked nucleic acid probes.
[So] Source:Int J Lab Hematol;38(6):e102-e106, 2016 Dec.
[Is] ISSN:1751-553X
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Leucemia Mielogênica Crônica BCR-ABL Positiva/diagnóstico
Oligonucleotídeos
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Análise Custo-Benefício
Seres Humanos
Neoplasia Residual/diagnóstico
Sondas de Ácido Nucleico
Reação em Cadeia da Polimerase em Tempo Real/economia
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Nucleic Acid Probes); 0 (Oligonucleotides); 0 (locked nucleic acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.1111/ijlh.12541


  7 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27153672
[Au] Autor:Niu L; Xu Z; Taylor JA
[Ad] Endereço:Division of Biostatistics and Bioinformatics, Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
[Ti] Título:RCP: a novel probe design bias correction method for Illumina Methylation BeadChip.
[So] Source:Bioinformatics;32(17):2659-63, 2016 Sep 01.
[Is] ISSN:1367-4811
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:MOTIVATION: The Illumina HumanMethylation450 BeadChip has been extensively utilized in epigenome-wide association studies. This array and its successor, the MethylationEPIC array, use two types of probes-Infinium I (type I) and Infinium II (type II)-in order to increase genome coverage but differences in probe chemistries result in different type I and II distributions of methylation values. Ignoring the difference in distributions between the two probe types may bias downstream analysis. RESULTS: Here, we developed a novel method, called Regression on Correlated Probes (RCP), which uses the existing correlation between pairs of nearby type I and II probes to adjust the beta values of all type II probes. We evaluate the effect of this adjustment on reducing probe design type bias, reducing technical variation in duplicate samples, improving accuracy of measurements against known standards, and retention of biological signal. We find that RCP is statistically significantly better than unadjusted data or adjustment with alternative methods including SWAN and BMIQ. AVAILABILITY: We incorporated the method into the R package ENmix, which is freely available from the Bioconductor website (https://www.bioconductor.org/packages/release/bioc/html/ENmix.html). CONTACT: niulg@ucmail.uc.edu SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
[Mh] Termos MeSH primário: Metilação de DNA
Análise de Sequência com Séries de Oligonucleotídeos
[Mh] Termos MeSH secundário: Viés
Genoma
Sequenciamento de Nucleotídeos em Larga Escala
Sondas de Ácido Nucleico
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleic Acid Probes)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE
[do] DOI:10.1093/bioinformatics/btw285


  8 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27054813
[Au] Autor:Thipmanee O; Numnuam A; Limbut W; Buranachai C; Kanatharana P; Vilaivan T; Hirankarn N; Thavarungkul P
[Ad] Endereço:Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Department of Chemistry, Faculty of Science, Prince of
[Ti] Título:Enhancing capacitive DNA biosensor performance by target overhang with application on screening test of HLA-B*58:01 and HLA-B*57:01 genes.
[So] Source:Biosens Bioelectron;82:99-104, 2016 Aug 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A highly sensitive label-free DNA biosensor based on PNA probes immobilized on a gold electrode was used to detect a hybridization event. The effect of a target DNA overhang on the hybridization efficiency was shown to enhance the detected signal and allowed detection at a very low concentration. The sensors performances were investigated with a complementary target that had the same length as the probe, and the signal was compared to the target DNAs with different lengths and overhangs. A longer target DNA overhang was found to provide a better response. When the overhang was on the electrode side the signal enhancement was greater than when the overhang was on the solution side due to the increased thickness of the sensing surface, hence produced a larger capacitance change. Using conformationally constrained acpcPNA probes, double stranded DNA was detected sensitively and specifically without any denaturing step. When two acpcPNA probes were applied for the screening test for the double stranded HLA-B*58:01 and HLA-B*57:01 genes that are highly similar, the method differentiated the two genes in all samples. Both purified and unpurified PCR products gave comparable results. This method would be potentially useful as a rapid screening test without the need for purification and denaturation of the PCR products.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
DNA/genética
Antígenos HLA-B/genética
[Mh] Termos MeSH secundário: Sequência de Bases
DNA/análise
Capacitância Elétrica
Seres Humanos
Hibridização de Ácido Nucleico/métodos
Sondas de Ácido Nucleico/química
Sondas de Ácido Nucleico/genética
Ácidos Nucleicos Peptídicos/química
Ácidos Nucleicos Peptídicos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HLA-B Antigens); 0 (HLA-B57 antigen); 0 (HLA-B58); 0 (Nucleic Acid Probes); 0 (Peptide Nucleic Acids); 9007-49-2 (DNA)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE


  9 / 530 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26866998
[Au] Autor:Cheng H; Qiu X; Zhao X; Meng W; Huo D; Wei H
[Ad] Endereço:Department of Biomedical Engineering, College of Engineering and Applied Sciences, Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing National Laboratory of Microstructures , Nanjing, Jiangsu 210093, China.
[Ti] Título:Functional Nucleic Acid Probe for Parallel Monitoring K(+) and Protoporphyrin IX in Living Organisms.
[So] Source:Anal Chem;88(5):2937-43, 2016 Mar 01.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parallel monitoring of K(+) and protoporphyrin IX (PPIX) is of vital importance because they are not only involved in a variety of biological processes but also closely linked to each other in numerous cellular pathways. However, there are currently no existing methods that can meet the requirements for parallel and in vivo detection of K(+) and PPIX in living organisms. Herein, we demonstrated a functional nucleic acid (FNA)-based technique for parallel monitoring of K(+) and PPIX in living animals. Specifically, the selected G-rich FNA probe was selectively induced to form a parallel G-quadruplex by K(+). The parallel G-quadruplex then remarkably enhanced the fluorescence of PPIX. Thus, by modulating the fluorescence "turn on" with the G-quadruplex and K(+)/PPIX, both K(+) and PPIX could be detected. After validating the developed method for selective and sensitive detection of K(+) and PPIX in vitro, their dynamic changes in living organisms (i.e., living brains and tumors) following various physiological and pathological processes were simultaneously monitored. The current study not only provides a general method for the detection of metal ions and bioactive molecules but also presents a way to investigate their synergistic functions in the regulation of various biological processes. It may also be helpful for improving the imaging and therapeutic efficacy of PPIX and 5-ALA.
[Mh] Termos MeSH primário: Sondas de Ácido Nucleico
Protoporfirinas/química
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nucleic Acid Probes); 0 (Protoporphyrins); C2K325S808 (protoporphyrin IX)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160212
[St] Status:MEDLINE
[do] DOI:10.1021/acs.analchem.5b04936


  10 / 530 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26843048
[Au] Autor:Nafa K; Hameed M; Arcila ME
[Ad] Endereço:Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA. NafaK@mskcc.org.
[Ti] Título:Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations.
[So] Source:Methods Mol Biol;1392:71-82, 2016.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The detection of clinically significant somatic mutations present at low level in a tissue sample represents a challenge in any laboratory. While several high sensitivity methods are described, the incorporation of these new techniques in a clinical lab may be difficult if the technology is not readily available or requires major changes in the workflow of the laboratory. Techniques that are robust and easily adapted to existing laboratory protocols are highly advantageous. In this chapter we describe the use of locked nucleic acid (LNA) probes to modify existing polymerase chain reaction (PCR)-based protocols which can then be sequenced by Sanger sequencing. LNA probes are used to enhance the sensitivity of Sanger sequencing to mutation frequencies below 1 %. The method is robust and is easily incorporated for assessment of any sample with low tumor content or low mutant allele burden.
[Mh] Termos MeSH primário: Análise Mutacional de DNA/métodos
Mutação
Sondas de Ácido Nucleico
Oligonucleotídeos
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Análise Mutacional de DNA/normas
Seres Humanos
Reação em Cadeia da Polimerase/normas
Sensibilidade e Especificidade
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleic Acid Probes); 0 (Oligonucleotides); 0 (locked nucleic acid)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160205
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-3360-0_8



página 1 de 53 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde