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[PMID]:28302536
[Au] Autor:Mendez-Pena JE; Sadow PM; Nose V; Hoang MP
[Ad] Endereço:Massachusetts General Hospital, Boston, MA 02114, USA.
[Ti] Título:RNA chromogenic in situ hybridization assay with clinical automated platform is a sensitive method in detecting high-risk human papillomavirus in squamous cell carcinoma.
[So] Source:Hum Pathol;63:184-189, 2017 May.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Detection of active human papillomavirus (HPV) is clinically important because its presence has been shown to correlate with favorable clinical outcomes and better response to treatment in oropharyngeal squamous cell carcinomas. Using a clinical automated platform, we compared the performance of commercially available HPV DNA and RNA in situ hybridization (ISH) probes in archival tissues of 57 squamous cell carcinomas. Importantly, a clinical automated platform gives (1) consistent and reproducible results for HPV ISH and (2) better standardization across clinical laboratories. Compared with polymerase chain reaction results, RNA ISH exhibited 93% concordance versus 81% of DNA ISH. RNA ISH was more sensitive than DNA ISH (100% versus 88%) and more specific (87% versus 74%). When only accounting for 2+-3+ positivity, sensitivity was 92% for RNA ISH versus 73% for DNA ISH, highlighting the ease of interpretation. p16 exhibited 96% sensitivity, whereas specificity was only 55%. In 3 cases, both RNA and DNA ISH were positive, whereas polymerase chain reaction results were negative, suggesting that ISH methods might be a more sensitive method. Performing on a clinical automated platform, RNA ISH is sensitive in determining high-risk HPV status in formalin-fixed, paraffin-embedded tissues and has the potential of being a standalone clinical test.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/virologia
Neoplasias de Cabeça e Pescoço/virologia
Hibridização In Situ
Técnicas de Diagnóstico Molecular
Papillomaviridae/genética
Infecções por Papillomavirus/virologia
RNA Viral/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Automação Laboratorial
Biomarcadores Tumorais/análise
Biópsia
Carcinoma de Células Escamosas/química
Carcinoma de Células Escamosas/patologia
Sondas de DNA de HPV
Feminino
Fixadores
Formaldeído
Neoplasias de Cabeça e Pescoço/química
Neoplasias de Cabeça e Pescoço/patologia
Testes de DNA para Papilomavírus Humano
Seres Humanos
Imuno-Histoquímica
Hibridização In Situ/normas
Masculino
Meia-Idade
Técnicas de Diagnóstico Molecular/normas
Infecções por Papillomavirus/patologia
Inclusão em Parafina
Reação em Cadeia da Polimerase
Valor Preditivo dos Testes
Sondas RNA
Reprodutibilidade dos Testes
Fixação de Tecidos/métodos
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (DNA Probes, HPV); 0 (Fixatives); 0 (RNA Probes); 0 (RNA, Viral); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE


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[PMID]:27874187
[Au] Autor:Kocsis A; Takács T; Jeney C; Schaff Z; Koiss R; Járay B; Sobel G; Pap K; Székely I; Ferenci T; Lai HC; Nyíri M; Benczik M
[Ad] Endereço:NEUMANN Diagnostics Ltd., Budapest, Hungary.
[Ti] Título:Performance of a new HPV and biomarker assay in the management of hrHPV positive women: Subanalysis of the ongoing multicenter TRACE clinical trial (n > 6,000) to evaluate POU4F3 methylation as a potential biomarker of cervical precancer and cancer.
[So] Source:Int J Cancer;140(5):1119-1133, 2017 Mar 01.
[Is] ISSN:1097-0215
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ongoing Triage and Risk Assessment of Cervical Precancer by Epigenetic Biomarker (TRACE) prospective, multicenter study aimed to provide a clinical evaluation of the CONFIDENCE™ assay, which comprises a human papillomavirus (HPV) DNA and a human epigenetic biomarker test. Between 2013 and 2015 over 6,000 women aged 18 or older were recruited in Hungary. Liquid-based cytology (LBC), high-risk HPV (hrHPV) DNA detection and single target host gene methylation test of the promoter sequence of the POU4F3 gene by quantitative methylation-specific polymerase chain reaction (PCR) were performed from the same liquid-based cytology sample. The current analysis is focused on the baseline cross-sectional clinical results of 5,384 LBC samples collected from subjects aged 25 years or older. The performance of the CONFIDENCE HPV™ test was found to be comparable to the cobas® HPV test with good agreement. When applying the CONFIDENCE Marker™ test alone in hrHPV positives, it showed significantly higher sensitivity with matching specificity compared to LBC-based triage. For CIN3+ histological endpoint in the age group of 25-65 and 30-65, the methylation test of POU4F3 achieved relative sensitivities of 1.74 (95% CI: 1.25-2.33) and 1.64 (95% CI: 1.08-2.27), respectively, after verification bias adjustment. On the basis of our findings, POU4F3 methylation as a triage test of hrHPV positives appears to be a noteworthy method. We can reasonably assume that its quantitative nature offers the potential for a more objective and discriminative risk assessment tool in the prevention and diagnostics of high-grade cervical intraepithelial neoplasia (CIN) lesions and cervical cancer.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/química
Proteínas de Homeodomínio/análise
Infecções por Papillomavirus/metabolismo
Lesões Pré-Cancerosas/metabolismo
Fator de Transcrição Brn-3C/análise
Displasia do Colo do Útero/metabolismo
Neoplasias do Colo do Útero/química
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Biomarcadores
Biomarcadores Tumorais
Carcinoma de Células Escamosas/virologia
Neoplasia Intraepitelial Cervical/química
Neoplasia Intraepitelial Cervical/virologia
Metilação de DNA
Sondas de DNA de HPV
DNA Viral/análise
Feminino
Proteínas de Homeodomínio/genética
Seres Humanos
Hungria/epidemiologia
Meia-Idade
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/virologia
Lesões Pré-Cancerosas/virologia
Regiões Promotoras Genéticas
Estudos Prospectivos
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Fator de Transcrição Brn-3C/genética
Triagem
Displasia do Colo do Útero/virologia
Neoplasias do Colo do Útero/virologia
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Biomarkers); 0 (Biomarkers, Tumor); 0 (DNA Probes, HPV); 0 (DNA, Viral); 0 (Homeodomain Proteins); 0 (POU4F3 protein, human); 0 (Transcription Factor Brn-3C)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1002/ijc.30534


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[PMID]:27613249
[Au] Autor:Fusconi M; Campo F; Gallo A; Zambetti G; Martellucci S; Seccia A; de Vincentiis M
[Ad] Endereço:Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy. Electronic address: Massimo.fusconi@libero.it.
[Ti] Título:Laryngeal Cancer, HPV DNA vs E6/E7 mRNA Test: A Systematic Review.
[So] Source:J Voice;31(2):248.e1-248.e5, 2017 Mar.
[Is] ISSN:1873-4588
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The reported range of involvement of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (SCC) is wide because of the methods used to detect HPV. DATA SOURCES: A computerized Medline study was carried out using the following as key words: "Papillomavirus Infections"[Mesh] and "Laryngeal Neoplasms"[Mesh]. MATERIALS AND METHODS: Studies that were included were written in English and reported results of HPV DNA with RNA in laryngeal SCC. RESULTS: There were six reported HPV mRNA extraction. Among these studies, Lewis et al reported that out of the 31 cases analyzed, only 2 were HPV DNA+ and of these only 1 was mRNA HPV+ (3%). Halec et al reported 102 cases of which 32 were HPV DNA+ cases and of which only 6 were mRNA+ (5%). Chernock et al reported 76 cases of which 13 were HPV DNA+ cases and of which 4 were mRNA+ (5%). Masand et al reported 8 cases of which 1 was HPV DNA+ case and none was mRNA+. Gheit et al reported 43 cases of which 4 were HPV DNA+ cases and of which 2 were mRNA+ (4%). Castellsagné et al reported 1042 cases of which 59 were HPV DNA+ case and of which 51 were mRNA+ (4.8%) CONCLUSIONS: When determining the role of HPV in laryngeal SCC, evidence of HPV DNA warrants further examination for E6/E7 mRNA as simple assays such as p16 are nonspecific in laryngeal SCC. Further studies of HPV and its role in laryngeal SCC are warranted.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/virologia
DNA Viral/genética
Neoplasias de Cabeça e Pescoço/virologia
Testes de DNA para Papilomavírus Humano
Neoplasias Laríngeas/virologia
Papillomaviridae/genética
Proteínas E7 de Papillomavirus/genética
Infecções por Papillomavirus/virologia
RNA Mensageiro/genética
RNA Viral/genética
[Mh] Termos MeSH secundário: Southern Blotting
Carcinoma de Células Escamosas/diagnóstico
Transformação Celular Viral
Sondas de DNA de HPV
Neoplasias de Cabeça e Pescoço/diagnóstico
Seres Humanos
Hibridização In Situ
Neoplasias Laríngeas/diagnóstico
Infecções por Papillomavirus/diagnóstico
Reação em Cadeia da Polimerase
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA Probes, HPV); 0 (DNA, Viral); 0 (Papillomavirus E7 Proteins); 0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160911
[St] Status:MEDLINE


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[PMID]:27647799
[Au] Autor:Chen K; Ouyang Y; Hillemanns P; Jentschke M
[Ad] Endereço:Department of Gynaecology and Obstetrics, Hannover Medical School, Hannover, Germany.
[Ti] Título:Excellent analytical and clinical performance of a dry self-sampling device for human papillomavirus detection in an urban Chinese referral population.
[So] Source:J Obstet Gynaecol Res;42(12):1839-1845, 2016 Dec.
[Is] ISSN:1447-0756
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:AIM: To evaluate the analytical and clinical effectiveness of cervicovaginal self-sampling with a dry sampling device (Evalyn Brush) for high-risk human papillomavirus (hr-HPV) testing and detection of cervical disease. METHODS: The study population consisted of 101 patients from a large gynecological outpatient clinic in Shanghai referred for abnormal cervical screening results and 101 women without cervical lesions. Self-samples obtained in the clinic and physician-collected cervical specimens (reference) were stored at -20 °C for 16-18 weeks and then transferred to 20 ml of ThinPrep medium and tested for hr-HPV using a multiplex real time polymerase chain reaction assay. All women had a colposcopic examination with a Pap smear and directed or random biopsies. RESULTS: High risk-HPV was detected in 92 patients (45.5%) with the self-collected cervicovaginal specimens and in 93 (46.0%) with the physician-collected cervical specimens, resulting in an agreement of 97.5% and a Kappa of 0.95 (95% confidence interval 0.91-0.99). Among all of the included women, 46 (22.8%) had cervical intraepithelial neoplasia grade 3 or worse (cervical intraepithelial neoplasia 3+). Hr-HPV was found in 43 of these patients (93.5%) with self-sampling and in 44 (95.7%) with the physician-collected specimens. CONCLUSIONS: Self-collected dry cervicovaginal samples transferred to ThinPrep medium and tested for hr-HPV using a clinically validated polymerase chain reaction assay showed very good agreement with physician-collected cervical specimens and a very high hr-HPV positivity rate for cervical intraepithelial neoplasia 3 +.
[Mh] Termos MeSH primário: Sondas de DNA de HPV
Detecção Precoce de Câncer/métodos
Infecções por Papillomavirus/diagnóstico
Neoplasias do Colo do Útero/virologia
[Mh] Termos MeSH secundário: Adulto
Idoso
China
Feminino
Seres Humanos
Meia-Idade
Papillomaviridae/genética
Infecções por Papillomavirus/genética
População Urbana
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Probes, HPV)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1111/jog.13132


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[PMID]:26481139
[Au] Autor:Okuma K; Yamashita H; Yokoyama T; Nakagawa K; Kawana K
[Ti] Título:Undetected human papillomavirus DNA and uterine cervical carcinoma: Association with cancer recurrence.
[So] Source:Strahlenther Onkol;192(1):55-62, 2016 Jan.
[Is] ISSN:1439-099X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The time course of human papillomavirus (HPV) DNA clearance was studied in patients with carcinoma of the cervix during follow-up after primary radical radiotherapy (RT). This study investigated the relationship between timing of HPV clearance and RT effectiveness. PATIENTS AND METHODS: A total of 71 consecutive patients who were treated for cervical cancer with primary radical radiotherapy and high-dose rate intracavitary brachytherapy with or without chemotherapy were enrolled in the study. Samples for HPV DNA examination were taken before (1) treatment, (2) every brachytherapy, and (3) every follow-up examination. The times when HPV DNA was undetected were analyzed for association with recurrence-free survival. RESULTS: HPV DNA was not detected in 13 patients (18 %) before RT. Of the 58 patients with HPV DNA detected before treatment, HPV DNA was not detected in 34 % during treatment and in 66 % after the treatment. Within 6 months after RT, HPV DNA was detected in 0 % of all patients. The patients were followed up for a median period of 43 months (range 7-70 months). In all, 20 patients were found to develop recurrence. The 3-year cumulative disease-free survival (DFS) rate was 71 ・} 5.4 % for all 71 patients. In multivariate analysis, DFS was significantly associated with HPV (detected vs. not detected) with a hazard ratio of 0.07 (95 % confidence interval 0.008-0.6, p = 0.009). CONCLUSION: In this study, patients in whom HPV was not detected had the worst prognosis. Six months after RT, HPV DNA was detected in 0 % of the patients. Patients in whom HPV DNA could not be detected before treatment need careful follow-up for recurrence and may be considered for additional, or alternative treatment.
[Mh] Termos MeSH primário: Adenocarcinoma/radioterapia
Braquiterapia
Carcinoma Adenoescamoso/radioterapia
Carcinoma de Células Escamosas/radioterapia
Sondas de DNA de HPV/análise
Recidiva Local de Neoplasia/radioterapia
Neoplasias do Colo do Útero/radioterapia
[Mh] Termos MeSH secundário: Adenocarcinoma/diagnóstico
Adenocarcinoma/mortalidade
Adenocarcinoma/patologia
Adulto
Idoso
Carcinoma Adenoescamoso/diagnóstico
Carcinoma Adenoescamoso/mortalidade
Carcinoma Adenoescamoso/patologia
Carcinoma de Células Escamosas/diagnóstico
Carcinoma de Células Escamosas/mortalidade
Carcinoma de Células Escamosas/patologia
Quimiorradioterapia
Intervalo Livre de Doença
Feminino
Seguimentos
Seres Humanos
Japão
Meia-Idade
Recidiva Local de Neoplasia/diagnóstico
Recidiva Local de Neoplasia/mortalidade
Recidiva Local de Neoplasia/patologia
Prognóstico
Retratamento
Neoplasias do Colo do Útero/diagnóstico
Neoplasias do Colo do Útero/mortalidade
Neoplasias do Colo do Útero/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Probes, HPV)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151021
[St] Status:MEDLINE


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[PMID]:26275464
[Au] Autor:Sánchez-Torices MS; Corrales-Millan R; Hijona-Elosegui JJ
[Ad] Endereço:Servicio de Otorrinolaringología, Complejo Hospitalario de Jaén, Jaén, España. Electronic address: marisolsancheztorices@gmail.com.
[Ti] Título:Oropharyngeal perinatal colonization by human papillomavirus.
[Ti] Título:Colonización orofaríngea perinatal por el virus del papiloma humano..
[So] Source:Acta Otorrinolaringol Esp;67(3):135-41, 2016 May-Jun.
[Is] ISSN:1988-3013
[Cp] País de publicação:Spain
[La] Idioma:eng; spa
[Ab] Resumo:INTRODUCTION AND OBJECTIVE: Human papillomavirus (HPV) infection is the most common human sexually transmitted disease. It is clinically relevant because this condition is necessary for the development of epithelial cervical cancer, and it is also a factor closely associated with the occurrence of diverse tumours and various benign and malignant lesions of the head and neck area. The infective mechanism in most of these cases is associated with sexual intercourse, but there is recent scientific evidence suggesting that HPV infection may also be acquired by other routes of infection not necessarily linked to sexual contact. One of them is vertical transmission from mother to child, either during pregnancy or at the time of delivery. The aim of our research was to study maternal-foetal HPV transmission during childbirth in detail, establishing the rate of oropharyngeal neonatal HPV in vaginal deliveries. METHOD: The presence and type of HPV viral DNA at the time of delivery in samples of maternal cervical secretions, amniotic fluid, venous cord blood samples and neonatal oropharynx in pregnant women (and their babies) were determined. RESULTS: The rate of oropharyngeal neonatal HPV colonization in vaginal deliveries was 58.24%. CONCLUSIONS: The maternal and neonatal HPV colonization mechanism is essentially, but not exclusively, transvaginal.
[Mh] Termos MeSH primário: Transmissão Vertical de Doença Infecciosa
Orofaringe/virologia
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/transmissão
Complicações Infecciosas na Gravidez/virologia
[Mh] Termos MeSH secundário: Adulto
Líquido Amniótico/virologia
Líquidos Corporais/virologia
Portador Sadio/virologia
Criança
Sondas de DNA de HPV
DNA Viral/análise
Feminino
Sangue Fetal/virologia
Seres Humanos
Imunocompetência
Recém-Nascido
Papillomaviridae/classificação
Infecções por Papillomavirus/virologia
Vacinas contra Papillomavirus
Gravidez
Vagina/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Probes, HPV); 0 (DNA, Viral); 0 (Papillomavirus Vaccines)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150816
[St] Status:MEDLINE


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[PMID]:26162490
[Au] Autor:Vargas-Hernández VM; Vargas-Aguilar VM; Tovar-Rodríguez JM
[Ad] Endereço:Dirección de Investigación, Hospital Juárez de México D.F., México. Electronic address: vvargashernandez@yahoo.com.mx.
[Ti] Título:[Primary cervical cancer screening].
[Ti] Título:Detección primaria del cáncer cervicouterino..
[So] Source:Cir Cir;83(5):448-53, 2015 Sep-Oct.
[Is] ISSN:0009-7411
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:Cervico-uterine cancer screening with cytology decrease incidence by more than 50%. The cause of this cancer is the human papilloma virus high risk, and requires a sensitive test to provide sufficient sensitivity and specificity for early detection and greater interval period when the results are negative. The test of the human papilloma virus high risk, is effective and safe because of its excellent sensitivity, negative predictive value and optimal reproducibility, especially when combined with liquid-based cytology or biomarkers with viral load, with higher sensitivity and specificity, by reducing false positives for the detection of cervical intraepithelial neoplasia grade 2 or greater injury, with excellent clinical benefits to cervical cancer screening and related infection of human papilloma virus diseases, is currently the best test for early detection infection of human papillomavirus and the risk of carcinogenesis.
[Mh] Termos MeSH primário: Detecção Precoce de Câncer/métodos
Neoplasias do Colo do Útero/prevenção & controle
[Mh] Termos MeSH secundário: Biomarcadores Tumorais
Neoplasia Intraepitelial Cervical/diagnóstico
Neoplasia Intraepitelial Cervical/epidemiologia
Neoplasia Intraepitelial Cervical/patologia
Neoplasia Intraepitelial Cervical/virologia
Sondas de DNA de HPV
Detecção Precoce de Câncer/tendências
Feminino
Genes Virais
Genes p16
Promoção da Saúde
Seres Humanos
Incidência
Recidiva Local de Neoplasia/diagnóstico
Proteínas Oncogênicas Virais/genética
Teste de Papanicolaou
Papillomaviridae/classificação
Papillomaviridae/isolamento & purificação
Papillomaviridae/patogenicidade
Infecções por Papillomavirus/diagnóstico
Infecções por Papillomavirus/epidemiologia
Infecções por Papillomavirus/prevenção & controle
Infecções por Papillomavirus/virologia
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Infecções Tumorais por Vírus/diagnóstico
Infecções Tumorais por Vírus/epidemiologia
Infecções Tumorais por Vírus/virologia
Neoplasias do Colo do Útero/diagnóstico
Neoplasias do Colo do Útero/epidemiologia
Neoplasias do Colo do Útero/patologia
Neoplasias do Colo do Útero/virologia
Esfregaço Vaginal
Carga Viral
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (DNA Probes, HPV); 0 (Oncogene Proteins, Viral)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151008
[Lr] Data última revisão:
151008
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150712
[St] Status:MEDLINE


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[PMID]:25976752
[Au] Autor:Mateos-Lindemann ML; Rubio MD; Benito-Berlinches A; Dominguez P
[Ad] Endereço:Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Madrid, Spain. Electronic address: mmateos.hrc@salud.madrid.org.
[Ti] Título:Performance of cobas 4800(®) and HPV genotype-specific distribution in opportunistic cervical cancer screening in Madrid.
[So] Source:Enferm Infecc Microbiol Clin;33(9):634-5, 2015 Nov.
[Is] ISSN:1578-1852
[Cp] País de publicação:Spain
[La] Idioma:eng
[Mh] Termos MeSH primário: Colo do Útero/virologia
Detecção Precoce de Câncer/métodos
Técnicas de Genotipagem/instrumentação
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Lesões Intraepiteliais Escamosas Cervicais/virologia
Neoplasias do Colo do Útero/virologia
Cervicite Uterina/virologia
Esfregaço Vaginal
[Mh] Termos MeSH secundário: Sondas de DNA de HPV
Detecção Precoce de Câncer/instrumentação
Feminino
Genótipo
Papillomavirus Humano 16/isolamento & purificação
Papillomavirus Humano 18/isolamento & purificação
Seres Humanos
Papillomaviridae/classificação
Papillomaviridae/genética
Infecções por Papillomavirus/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real/instrumentação
Espanha/epidemiologia
Lesões Intraepiteliais Escamosas Cervicais/epidemiologia
Neoplasias do Colo do Útero/epidemiologia
Cervicite Uterina/epidemiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; LETTER; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA Probes, HPV)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE


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[PMID]:25724573
[Au] Autor:Stoddard DG; Keeney MG; Gao G; Smith DI; García JJ; O'Brien EK
[Ad] Endereço:Department of Otorhinolaryngology, Mayo Clinic, Rochester, Minnesota, USA stoddard.david@mayo.edu.
[Ti] Título:Transcriptional activity of HPV in inverted papilloma demonstrated by in situ hybridization for E6/E7 mRNA.
[So] Source:Otolaryngol Head Neck Surg;152(4):752-8, 2015 Apr.
[Is] ISSN:1097-6817
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Assess human papilloma virus (HPV) transcriptional activity in inverted Schneiderian papillomas (IPs). STUDY DESIGN: Case series with chart review. SETTING: Academic tertiary care center. SUBJECTS AND METHODS: Retrospective clinicopathologic review of 19 cases of IP in patients undergoing surgical excision from 1995 to 2013 at Mayo Clinic in Rochester, Minnesota. Surgical pathology archival material was histopathologically reviewed using hematoxylin and eosin-stained slides. Formalin-fixed, paraffin-embedded material from each case was evaluated for p16 expression using immunohistochemistry as well as HPV DNA and E6/E7 messenger RNA (mRNA) transcription using polymerase chain reaction (PCR) and in situ hybridization (via RNAscope technology), respectively. RESULTS: Eight patients were female (42%), with an average age of 53 years (range, 23-82 years). Three demonstrated malignancy, and 5 subsequently recurred. Average follow-up was 49 months (range, 0-200 months), and 1 patient died from squamous cell carcinoma arising from the IP. RNAscope detected HPV mRNA transcripts exclusively within IP in 100% of cases; however, in 11 patients (58%), less than 1% of cells exhibited transcriptional activity. Only 2 of 19 cases (11%) demonstrated mRNA activity in 50% or more cells. HPV DNA was detected in only 2 specimens by PCR. CONCLUSIONS: This study reveals wide prevalence but limited transcriptional activity of HPV in IP. No correlation between HPV transcriptional activity and progression, recurrence, or malignant transformation was identified. These data suggest that transcription of HPV may contribute to the pathogenesis of IP, but prospective data are needed to definitively demonstrate this connection. These results also suggest that RNAscope may be more sensitive than PCR in detecting HPV activity in IP.
[Mh] Termos MeSH primário: Neoplasias Nasais/virologia
Proteínas Oncogênicas Virais/genética
Papiloma Invertido/virologia
Papillomaviridae/genética
RNA Mensageiro/metabolismo
Ativação Transcricional
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Inibidor p16 de Quinase Dependente de Ciclina
Sondas de DNA de HPV
DNA Viral/análise
DNA Viral/genética
Feminino
Seres Humanos
Hibridização In Situ
Masculino
Meia-Idade
Mucosa Nasal/patologia
Mucosa Nasal/virologia
Proteínas de Neoplasias/metabolismo
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/genética
Estudos Retrospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclin-Dependent Kinase Inhibitor p16); 0 (DNA Probes, HPV); 0 (DNA, Viral); 0 (Neoplasm Proteins); 0 (Oncogene Proteins, Viral); 0 (P16 protein, human); 0 (RNA, Messenger)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150301
[St] Status:MEDLINE
[do] DOI:10.1177/0194599815571285


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[PMID]:25701900
[Au] Autor:Padilla-España L; Millán Cayetano JF; García Montero P
[Ad] Endereço:Servicio de Dermatología, Hospital Costa del Sol, Marbella, Málaga, España. Electronic address: eplaura85@hotmail.com.
[Ti] Título:Detection of Oncogenic Human Papillomavirus Genotypes: A Useful Screening Tool for High-Grade Anal Intraepithelial Neoplasia.
[So] Source:Actas Dermosifiliogr;106(10):835-6, 2015 Dec.
[Is] ISSN:1578-2190
[Cp] País de publicação:Spain
[La] Idioma:eng; spa
[Mh] Termos MeSH primário: Neoplasias do Ânus/virologia
Carcinoma in Situ/virologia
Detecção Precoce de Câncer/métodos
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/virologia
[Mh] Termos MeSH secundário: Algoritmos
Neoplasias do Ânus/epidemiologia
Carcinoma in Situ/epidemiologia
Comorbidade
Sondas de DNA de HPV
Feminino
Genótipo
Infecções por HIV/epidemiologia
Seres Humanos
Masculino
Papillomaviridae/genética
Infecções por Papillomavirus/epidemiologia
Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Probes, HPV)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150223
[St] Status:MEDLINE



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