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  1 / 12949 MEDLINE  
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[PMID]:29256499
[Au] Autor:Zhang JX; Fang JZ; Duan W; Wu LR; Zhang AW; Dalchau N; Yordanov B; Petersen R; Phillips A; Zhang DY
[Ad] Endereço:Department of Bioengineering, Rice University, Houston, Texas 77030, USA.
[Ti] Título:Predicting DNA hybridization kinetics from sequence.
[So] Source:Nat Chem;10(1):91-98, 2018 01.
[Is] ISSN:1755-4349
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hybridization is a key molecular process in biology and biotechnology, but so far there is no predictive model for accurately determining hybridization rate constants based on sequence information. Here, we report a weighted neighbour voting (WNV) prediction algorithm, in which the hybridization rate constant of an unknown sequence is predicted based on similarity reactions with known rate constants. To construct this algorithm we first performed 210 fluorescence kinetics experiments to observe the hybridization kinetics of 100 different DNA target and probe pairs (36 nt sub-sequences of the CYCS and VEGF genes) at temperatures ranging from 28 to 55 °C. Automated feature selection and weighting optimization resulted in a final six-feature WNV model, which can predict hybridization rate constants of new sequences to within a factor of 3 with ∼91% accuracy, based on leave-one-out cross-validation. Accurate prediction of hybridization kinetics allows the design of efficient probe sequences for genomics research.
[Mh] Termos MeSH primário: DNA/química
Modelos Teóricos
Hibridização de Ácido Nucleico
[Mh] Termos MeSH secundário: Algoritmos
Genoma Humano
Seres Humanos
Cinética
Conformação de Ácido Nucleico
Sondas de Oligonucleotídeos
Valor Preditivo dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Oligonucleotide Probes); 9007-49-2 (DNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1038/nchem.2877


  2 / 12949 MEDLINE  
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[PMID]:27770356
[Au] Autor:Hernández-Castellano S; Nic-Can GI; De-la-Peña C
[Ad] Endereço:Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, Merida, Yucatán, 97200, Mexico.
[Ti] Título:Localization of miRNAs by In Situ Hybridization in Plants Using Conventional Oligonucleotide Probes.
[So] Source:Methods Mol Biol;1456:51-62, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Among the epigenetic mechanisms studied with a greater interest in the last decade are the microRNAs (miRNAs). These small noncoding RNA sequences that are approximately 17-22 nucleotides in length play an essential role in many biological processes of various organisms, including plants. The analysis of spatiotemporal expression of miRNAs provides a better understanding of the role of these small molecules in plant development, cell differentiation, and other processes; but such analysis is also an important method for the validation of biological functions. In this work, we describe the optimization of an efficient protocol for the spatiotemporal analysis of miRNA by in situ hybridization using different plant tissues embedded in paraffin. Instead of LNA-modified probes that are typically used for this work, we use conventional oligonucleotide probes that yield a high specificity and clean distribution of miRNAs.
[Mh] Termos MeSH primário: Hibridização In Situ
MicroRNAs/genética
MicroRNAs/metabolismo
Sondas de Oligonucleotídeos
RNA de Plantas
[Mh] Termos MeSH secundário: Imuno-Histoquímica/métodos
Hibridização In Situ/métodos
Inclusão em Parafina
Fixação de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Oligonucleotide Probes); 0 (RNA, Plant)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  3 / 12949 MEDLINE  
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[PMID]:28957362
[Au] Autor:Ito T; Suzaki K
[Ad] Endereço:Division of Grape and Persimmon Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Higashihiroshima, Hiroshima, Japan.
[Ti] Título:Universal detection of phytoplasmas and Xylella spp. by TaqMan singleplex and multiplex real-time PCR with dual priming oligonucleotides.
[So] Source:PLoS One;12(9):e0185427, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phytoplasmas and Xylella spp. are bacteria that cause many economically important plant diseases worldwide. TaqMan probe-based quantitative real-time polymerase chain reaction (qPCR) assays have been utilized to universally detect phytoplasmas or Xylella fastidiosa. To develop a superior universal qPCR method, we used a dual priming oligonucleotide (DPO) with two annealing sites as a reverse primer to target the well-conserved bacterial 16S rDNA. The new qPCR assays universally detected various species of phytoplasmas and subspecies of X. fastidiosa as well as Xylella taiwanensis, and generally showed superior threshold cycle values when amplifying specific or non-specific products compared to current universal qPCR assays. The proposed qPCR assays were integrated to develop a multiplex qPCR assay that simultaneously detected phytoplasmas, Xylella spp., and an internal plant DNA positive control within 1 hour. This assay could detect a minimum of ten bacterial cells and was compatible with crude extractions used in the rapid screening of various plants. The amplicons were of sufficient lengths to be directly sequenced for preliminary identification, and the primers could be used in universal conventional PCR assays. Additionally, reverse DPO primers can be utilized to improve other probe-based qPCR assays.
[Mh] Termos MeSH primário: Primers do DNA/genética
Reação em Cadeia da Polimerase Multiplex/métodos
Phytoplasma/genética
Phytoplasma/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Xylella/genética
Xylella/isolamento & purificação
[Mh] Termos MeSH secundário: Misturas Complexas
Sondas de Oligonucleotídeos/genética
Vitis/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (DNA Primers); 0 (Oligonucleotide Probes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185427


  4 / 12949 MEDLINE  
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[PMID]:28698239
[Au] Autor:Gaspar I; Wippich F; Ephrussi A
[Ad] Endereço:Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg 69117, Germany.
[Ti] Título:Enzymatic production of single-molecule FISH and RNA capture probes.
[So] Source:RNA;23(10):1582-1591, 2017 Oct.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arrays of singly labeled short oligonucleotides that hybridize to a specific target revolutionized RNA biology, enabling quantitative, single-molecule microscopy analysis and high-efficiency RNA/RNP capture. Here, we describe a simple and efficient method that allows flexible functionalization of inexpensive DNA oligonucleotides by different fluorescent dyes or biotin using terminal deoxynucleotidyl transferase and custom-made functional group conjugated dideoxy-UTP. We show that (i) all steps of the oligonucleotide labeling-including conjugation, enzymatic synthesis, and product purification-can be performed in a standard biology laboratory, (ii) the process yields >90%, often >95% labeled product with minimal carryover of impurities, and (iii) the oligonucleotides can be labeled with different dyes or biotin, allowing single-molecule FISH, RNA affinity purification, and Northern blot analysis to be performed.
[Mh] Termos MeSH primário: DNA Nucleotidilexotransferase/metabolismo
Hibridização in Situ Fluorescente/métodos
Sondas RNA/química
[Mh] Termos MeSH secundário: Animais
Biotina
Didesoxinucleotídeos/química
Didesoxinucleotídeos/metabolismo
Drosophila melanogaster/genética
Feminino
Corantes Fluorescentes/química
Sondas de Oligonucleotídeos/química
Oligonucleotídeos/química
Ovário/fisiologia
Sondas RNA/metabolismo
Nucleotídeos de Uracila/química
Nucleotídeos de Uracila/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dideoxynucleotides); 0 (Fluorescent Dyes); 0 (Oligonucleotide Probes); 0 (Oligonucleotides); 0 (RNA Probes); 0 (Uracil Nucleotides); 6SO6U10H04 (Biotin); 84445-38-5 (2',3'-dideoxyuridine-5'-triphosphate); EC 2.7.7.31 (DNA Nucleotidylexotransferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1261/rna.061184.117


  5 / 12949 MEDLINE  
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[PMID]:28522268
[Au] Autor:Yokoyama C; Nakamoto K; Ueno Y
[Ad] Endereço:Course of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
[Ti] Título:Design and synthesis of novel photoinduced electron transfer-based hybridization probes.
[So] Source:Bioorg Med Chem;25(13):3574-3582, 2017 Jul 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Photoinduced electron transfer (PeT)-based hybridization probe is a linear and quencher-free oligonucleotide (ON) probe for DNA or RNA detection. In this report, we designed and synthesized novel adenosine analogues for PeT-based hybridization probe. In particular, the analogue containing a piperazinomethyl moiety showed effective quenching property under physiological conditions. When the probe containing the analogue was hybridized with a complementary DNA or RNA, the fluorescence increased 3- or 4-fold, respectively, compared to the single-stranded state.
[Mh] Termos MeSH primário: Adenosina/química
DNA/análise
Desenho de Drogas
Sondas de Oligonucleotídeos/química
RNA/análise
[Mh] Termos MeSH secundário: Adenosina/análogos & derivados
Relação Dose-Resposta a Droga
Transporte de Elétrons
Fluorescência
Estrutura Molecular
Sondas de Oligonucleotídeos/síntese química
Processos Fotoquímicos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotide Probes); 63231-63-0 (RNA); 9007-49-2 (DNA); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE


  6 / 12949 MEDLINE  
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[PMID]:28438642
[Au] Autor:Srinivasan V; Stedtfeld RD; Tourlousse DM; Baushke SW; Xin Y; Miller SM; Pham T; Rouillard JM; Gulari E; Tiedje JM; Hashsham SA
[Ad] Endereço:Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, United States.
[Ti] Título:Diagnostic microarray for 14 water and foodborne pathogens using a flatbed scanner.
[So] Source:J Microbiol Methods;139:15-21, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Parallel detection approaches are of interest to many researchers interested in identifying multiple water and foodborne pathogens simultaneously. Availability and cost-effectiveness are two key factors determining the usefulness of such approaches for laboratories with limited resources. In this study, we developed and validated a high-density microarray for simultaneous screening of 14 bacterial pathogens using an approach that employs gold labeling with silver enhancement (GLS) protocol. In total, 8887 probes (50-mer) were designed using an in-house database of virulence and marker genes (VMGs), and synthesized in quadruplicate on glass slides using an in-situ synthesis technology. Target VMG amplicons were obtained using multiplex polymerase chain reaction (PCR), labeled with biotin, and hybridized to the microarray. The signals generated after gold deposition and silver enhancement, were quantified using a flatbed scanner having 2-µm resolution. Data analysis indicated that reliable presence/absence calls could be made, if: i) over four probes were used per gene, ii) the signal-to-noise ratio (SNR) cutoff was greater than or equal to two, and iii) the positive fraction (PF), i.e., number of probes with SNR≥2 for a given VMG was greater than 0.75. Hybridization of the array with blind samples resulted in 100% correct calls, and no false positive. Because amplicons were obtained by multiplex PCR, sensitivity of this method is similar to PCR. This assay is an inexpensive and reliable technique for high throughput screening of multiple pathogens.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Microbiologia de Alimentos
Reação em Cadeia da Polimerase Multiplex/métodos
Análise de Sequência com Séries de Oligonucleotídeos
Microbiologia da Água
[Mh] Termos MeSH secundário: Bactérias/genética
Bactérias/patogenicidade
Ouro/química
Processamento de Imagem Assistida por Computador/instrumentação
Processamento de Imagem Assistida por Computador/métodos
Reação em Cadeia da Polimerase Multiplex/instrumentação
Hibridização de Ácido Nucleico
Análise de Sequência com Séries de Oligonucleotídeos/instrumentação
Análise de Sequência com Séries de Oligonucleotídeos/métodos
Sondas de Oligonucleotídeos
Salmonella/genética
Salmonella/isolamento & purificação
Shigella/genética
Shigella/isolamento & purificação
Shigella/patogenicidade
Prata/química
Yersinia/genética
Yersinia/isolamento & purificação
Yersinia/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Oligonucleotide Probes); 3M4G523W1G (Silver); 7440-57-5 (Gold)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


  7 / 12949 MEDLINE  
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[PMID]:28325127
[Au] Autor:Pretzl B; Paul J; Krigar DM; Uhlmann L; Eickholz P; Dannewitz B
[Ad] Endereço:a Section of Periodontology, Department of Conservative Dentistry Clinic for Oral, Dental and Maxillofacial Diseases , University Hospital Heidelberg , Heidelberg , Germany.
[Ti] Título:Reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes.
[So] Source:Acta Odontol Scand;75(4):302-307, 2017 May.
[Is] ISSN:1502-3850
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The aim was to evaluate the intra-test agreement of pooled samples from the deepest periodontal pocket of each quadrant with a commercially available test kit based on hybridization of 16S rRNA. MATERIAL AND METHODS: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate vials in order to detect and compare counts of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Cohen's κ and interclass correlation coefficients were calculated to judge intra-test agreement. RESULTS: Cohen's κ for detection and counts of Tannerella forsythia and Treponema denticola showed a perfect agreement. Porphyromonas ginigivalis was identified in both tests with a substantial agreement, whereas detection of Aggregatibacter actinomycetemcomitans varied in eight patients resulting in a good agreement. Possible confounding factors could not be identified statistically. CONCLUSION: Test results of the commercial 16S rRNA test are perfectly reproducible regarding detection of red complex pathogens. Intra-test agreement concerning detection of Aggregatibacter actinomycetemcomitans was less favorable. CLINICAL RELEVANCE: Detection of certain periodontal pathogens may alter the treatment and lead to prescription of antibiotics parallel to mechanical debridement. It is quite important not to use antibiotics excessively. Thus, the basis for decision-making in favor of antibiotics should be solid.
[Mh] Termos MeSH primário: Carga Bacteriana/classificação
Periodontite Crônica/microbiologia
Placa Dentária/microbiologia
Bactérias Gram-Negativas/isolamento & purificação
Sondas de Oligonucleotídeos
Bolsa Periodontal/microbiologia
[Mh] Termos MeSH secundário: Seres Humanos
Bolsa Periodontal/classificação
Porphyromonas gingivalis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotide Probes)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1080/00016357.2017.1303192


  8 / 12949 MEDLINE  
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[PMID]:28279557
[Au] Autor:Krasheninina OA; Lomzov AA; Fishman VS; Novopashina DS; Venyaminova AG
[Ad] Endereço:Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev Ave., Novosibirsk 630090, Russia; Novosibirsk State University, 2 Pirogov str., Novosibirsk 630090, Russia. Electronic address: OKrasheninina@gmail.com.
[Ti] Título:Rational design and studies of excimer forming novel dual probes to target RNA.
[So] Source:Bioorg Med Chem;25(7):2244-2250, 2017 Apr 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this paper, we report structure-based rational design and physico-chemical and biological studies of novel pyrene excimer forming dual probes for visualization of intracellular RNAs. Herein, the probes based on 2'-O-methyl RNA with linkers of different structure and length between pyrene moiety and ribose are studied with respect to their hybridization and spectral properties. We found optimal linkers that provide more intense excimer emission (at ∼480nm) of RNA-bound probes; particularly, the length of the linker arm of the 3'-component of dual probes plays a key role in formation of pyrene excimer. Calculated molecular dynamics trajectories and probability distributions of pyrene-pyrene dimer formation upon hybridization of the dual probes with RNA target are in agreement with the obtained fluorescence spectroscopy data for the corresponding duplexes. Our study demonstrates the excellent binding properties of new dual probes to structured RNA and their feasibility for the visualization of intracellular RNA targets.
[Mh] Termos MeSH primário: Desenho de Drogas
Sondas de Oligonucleotídeos/química
RNA/química
[Mh] Termos MeSH secundário: Espectrometria de Fluorescência
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotide Probes); 63231-63-0 (RNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE


  9 / 12949 MEDLINE  
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[PMID]:28263762
[Au] Autor:Cerníková L; Vitásková E; Nagy A
[Ad] Endereço:State Veterinary Institute Prague, Prague, Czechia; University of Veterinary and Pharmaceutical Sciences, Department of Biology and Wildlife Diseases, Brno, Czechia. Electronic address: len.cernikova@gmail.com.
[Ti] Título:Development and evaluation of TaqMan real-time PCR assay for detection of beak and feather disease virus.
[So] Source:J Virol Methods;244:55-60, 2017 Jun.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Psittacine beak and feather disease (PBFD) is one of the most significant viral diseases in psittacine birds. The aim of the presented study was to develop a highly specific and sensitive TaqMan real-time PCR assay for universal detection of beak and feather disease virus (BFDV). Primers and a hydrolysis probe were selected on the highly conserved regions belonging to the ORF1 of the BFDV genome which were identified by aligning 814 genomic sequences downloaded from the GenBank database. The evaluation of the reaction parameters suggested a reaction efficiency of 97.1%, with consistent detection of 10 virus copies/µl of nucleic acid extract. The low values of standard deviation and coefficient of variation indicate a high degree of reproducibility and repeatability. The diagnostic applicability of the assay was proven on 36 BFDV positive and 107 negative specimens of psittacine origin representing 28 species. The assay showed a 100% ability to detect distinct genetic variants of the virus. Our data suggest that the presented TaqMan real-time PCR represents a specific, sensitive and reliable assay facilitating the molecular detection of BFDV.
[Mh] Termos MeSH primário: Doenças das Aves/diagnóstico
Doenças das Aves/virologia
Infecções por Circoviridae/veterinária
Circovirus/isolamento & purificação
Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Medicina Veterinária/métodos
[Mh] Termos MeSH secundário: Animais
Aves
Infecções por Circoviridae/diagnóstico
Infecções por Circoviridae/virologia
Circovirus/genética
Primers do DNA/genética
Sondas de Oligonucleotídeos/genética
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (Oligonucleotide Probes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE


  10 / 12949 MEDLINE  
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[PMID]:28242171
[Au] Autor:Kumar P; Sharma PK; Nielsen P
[Ad] Endereço:Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, 5230 Odense M, Denmark.
[Ti] Título:Synthesis, hybridization and fluorescence properties of a 2'-C-pyrene-triazole modified arabino-uridine nucleotide.
[So] Source:Bioorg Med Chem;25(7):2084-2090, 2017 Apr 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new pyrene-modified nucleotide monomer is introduced, wherein pyrene is attached to the 2'-position of arabino-uridine through a triazolemethyl linker. When the monomer is introduced into oligonucleotides, very stable DNA duplexes and three way junctions are obtained. An oligonucleotide featuring two modifications in the center shows four-fold increase in the intensity of the pyrene excimer signal on hybridization with an RNA target but not with a DNA target. The new nucleotide monomer has potential in DNA invader probes as well as in RNA targeting and detection.
[Mh] Termos MeSH primário: Nucleotídeos/química
Pirenos/química
Triazóis/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Fluorescência
Sondas de Oligonucleotídeos
Espectroscopia de Prótons por Ressonância Magnética
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleotides); 0 (Oligonucleotide Probes); 0 (Pyrenes); 0 (Triazoles); 9E0T7WFW93 (pyrene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE



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