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[PMID]:28461450
[Au] Autor:Rothstein DM; Lazinski D; Osburne MS; Sonenshein AL
[Ad] Endereço:Graduate Program in Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA.
[Ti] Título:A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.
[So] Source:J Bacteriol;199(14), 2017 07 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mutants of that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the gene, which encodes a phosphatase that indirectly activates SigB, the stress-responsive RNA polymerase sigma factor. The mutation appeared to cause RsbU to be hyperactive, because the mutants were more resistant than the parent strain to ethanol stress. In support of this hypothesis, pseudorevertants that regained wild-type levels of sporulation at high temperature had secondary mutations that prevented expression of the mutant gene. The properties of these RsbU mutants support the idea that activation of SigB diminishes the bacterium's ability to sporulate. Most bacterial species encode multiple RNA polymerase promoter recognition subunits (sigma factors). Each sigma factor directs RNA polymerase to different sets of genes; each gene set typically encodes proteins important for responses to specific environmental conditions, such as changes in temperature, salt concentration, and nutrient availability. A selection for mutants of that are temperature sensitive for RNA synthesis during sporulation unexpectedly yielded strains with a point mutation in , a gene that encodes a protein that normally activates sigma factor B (SigB) under conditions of salt stress. The mutation appears to cause RsbU, and therefore SigB, to be active inappropriately, thereby inhibiting, directly or indirectly, the ability of the cells to transcribe sporulation genes.
[Mh] Termos MeSH primário: Bacillus subtilis/metabolismo
Proteínas de Bactérias/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
Monoéster Fosfórico Hidrolases/metabolismo
RNA Bacteriano/biossíntese
Esporos Bacterianos/fisiologia
[Mh] Termos MeSH secundário: Bacillus subtilis/genética
Proteínas de Bactérias/genética
Etanol/farmacologia
Genoma Bacteriano
Temperatura Alta
Mutação
Fosfatos/metabolismo
Monoéster Fosfórico Hidrolases/genética
Radioisótopos de Fósforo
Estresse Fisiológico/efeitos dos fármacos
Estresse Fisiológico/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Phosphates); 0 (Phosphorus Radioisotopes); 0 (RNA, Bacterial); 3K9958V90M (Ethanol); EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.1.3.3 (RsbU protein, Bacillus subtilis)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  2 / 24062 MEDLINE  
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[PMID]:29351548
[Au] Autor:Geltsch N; Elek Z; Manczinger L; Vágvölgyi C; Moskát C
[Ad] Endereço:MTA-ELTE-MTM Ecology Research Group, a joint research group of the Hungarian Academy of Sciences, the Biological Institute of the Eötvös Loránd University and the Hungarian Natural History Museum, Budapest, Hungary.
[Ti] Título:Common cuckoos (Cuculus canorus) affect the bacterial diversity of the eggshells of their great reed warbler (Acrocephalus arundinaceus) hosts.
[So] Source:PLoS One;13(1):e0191364, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The common cuckoo (Cuculus canorus) is an avian brood parasite, laying its eggs in the nests of other bird species, where these hosts incubate the parasitic eggs, feed and rear the nestlings. The appearance of a cuckoo egg in a host nest may change the bacterial community in the nest. This may have consequences on the hatchability of host eggs, even when hosts reject the parasitic egg, typically within six days after parasitism. The present study revealed the bacterial community of cuckoo eggshells and those of the great reed warbler (Acrocephalus arundinaceus), one of the main hosts of cuckoos. We compared host eggs from non-parasitized clutches, as well as host and cuckoo eggs from parasitized clutches. As incubation may change bacterial assemblages on eggshells, we compared these egg types in two stages: the egg-laying stage, when incubation has not been started, and the mid-incubation stage (ca. on days 5-7 in incubation), where heat from the incubating female dries eggshells. Our results obtained by the 16S rRNA gene sequencing technique showed that fresh host and cuckoo eggs had partially different bacterial communities, but they became more similar during incubation in parasitized nests. Cluster analysis revealed that fresh cuckoo eggs and incubated host eggs in unparasitized nests (where no cuckoo effect could have happened) were the most dissimilar from the other groups of eggs. Cuckoo eggs did not reduce the hatchability of great reed warbler eggs. Our results on the cuckoo-great reed warbler relationship supported the idea that brood parasites may change bacterial microbiota in the host nest. Further studies should reveal how bacterial communities of cuckoo eggshells may vary by host-specific races (gentes) of cuckoos.
[Mh] Termos MeSH primário: Aves/microbiologia
Casca de Ovo/microbiologia
Aves Canoras/microbiologia
Aves Canoras/parasitologia
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Aves/fisiologia
Feminino
Interações Hospedeiro-Parasita/genética
Microbiota/genética
Comportamento de Nidação
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Aves Canoras/fisiologia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191364


  3 / 24062 MEDLINE  
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[PMID]:28741379
[Au] Autor:Choi JW; Um JH; Cho JH; Lee HJ
[Ad] Endereço:1 Department of Microbiology and Immunology, Kyungpook National University School of Dentistry, Daegu 41940, Korea.
[Ti] Título:Tiny RNAs and their voyage via extracellular vesicles: Secretion of bacterial small RNA and eukaryotic microRNA.
[So] Source:Exp Biol Med (Maywood);242(15):1475-1481, 2017 09.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:MicroRNAs are small non-coding RNAs that bind to the 3'-untranslated region of target mRNAs and have transcriptional or translational inhibitory function in eukaryotes. Before microRNAs were widely known, bacterial non-coding small RNAs around 50-200 nt in length were discovered whose mechanism of action resembled that of microRNAs. Recently, RNAs that are of similar size to or smaller than microRNAs have been discovered in bacteria and indeed, this class of small RNAs have been found throughout all domains of life. Moreover, recent findings suggest that these tiny RNAs can be released via extracellular vesicles (such as exosomes in eukaryotes and outer membrane vesicles in bacteria), which in turn heralds a new field of research, interkingdom communication. This review discusses two similar classes of small RNAs in evolutionarily distinct eukaryotes and bacteria. In addition to their biogenesis and regulation, we discuss small RNA vehicles and their secretion. Impact statement The possible endogenous functions of small RNAs such as regulatory small RNAs in bacteria and microRNAs in eukaryotes have been extensively studied since they were first discovered. However, their powerful functions should not be seen as limited to their cells of origin. Recently, several papers have demonstrated that small RNAs function as signaling molecules between cells. This is possible because small RNAs can be shuttled around after being incorporated into environmentally protective extracellular vesicles. It is now clearly plausible that secreted small RNAs can regulate other types of cells through biofluids. Given their "common molecule" status, the role of small RNAs in mediating bacteria-human crosstalk is an emerging and competitive area of genetic research. This review provides insight into the function of small RNAs in intercellular and even interkingdom communication.
[Mh] Termos MeSH primário: Vesículas Extracelulares/metabolismo
Pequeno RNA não Traduzido/secreção
[Mh] Termos MeSH secundário: Bactérias/metabolismo
Eucariotos/metabolismo
RNA Bacteriano/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Small Untranslated)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1177/1535370217723166


  4 / 24062 MEDLINE  
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[PMID]:29329346
[Au] Autor:Nycz BT; Dominguez SR; Friedman D; Hilden JM; Ir D; Robertson CE; Frank DN
[Ad] Endereço:Division of Adult Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, United States of America.
[Ti] Título:Evaluation of bloodstream infections, Clostridium difficile infections, and gut microbiota in pediatric oncology patients.
[So] Source:PLoS One;13(1):e0191232, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bloodstream infections (BSI) and Clostridium difficile infections (CDI) in pediatric oncology/hematology/bone marrow transplant (BMT) populations are associated with significant morbidity and mortality. The objective of this study was to explore possible associations between altered microbiome composition and the occurrence of BSI and CDI in a cohort of pediatric oncology patients. Stool samples were collected from all patients admitted to the pediatric oncology floor from Oct.-Dec. 2012. Bacterial profiles from patient stools were determined by bacterial 16S rRNA gene profiling. Differences in overall microbiome composition were assessed by a permutation-based multivariate analysis of variance test, while differences in the relative abundances of specific taxa were assessed by Kruskal-Wallis tests. At admission, 9 of 42 patients (21%) were colonized with C. difficile, while 6 of 42 (14%) subsequently developed a CDI. Furthermore, 3 patients (7%) previously had a BSI and 6 patients (14%) subsequently developed a BSI. Differences in overall microbiome composition were significantly associated with disease type (p = 0.0086), chemotherapy treatment (p = 0.018), BSI following admission from any cause (p < 0.0001) or suspected gastrointestinal organisms (p = 0.00043). No differences in baseline microbiota were observed between individuals who did or did not subsequently develop C. difficile infection. Additionally, multiple bacterial groups varied significantly between subjects with post-admission BSI compared with no BSI. Our results suggest that differences in gut microbiota not only are associated with type of cancer and chemotherapy, but may also be predictive of subsequent bloodstream infection.
[Mh] Termos MeSH primário: Bacteriemia/complicações
Infecções por Clostridium/complicações
Microbioma Gastrointestinal
Neoplasias/complicações
Neoplasias/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Bacteriemia/microbiologia
Proteínas de Bactérias/genética
Toxinas Bacterianas/genética
Biodiversidade
Criança
Pré-Escolar
Infecções por Clostridium/microbiologia
Clostridium difficile/genética
Estudos de Coortes
Estudos Transversais
Fezes/microbiologia
Feminino
Microbioma Gastrointestinal/genética
Genes Bacterianos
Seres Humanos
Masculino
Valor Preditivo dos Testes
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (toxB protein, Clostridium difficile)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191232


  5 / 24062 MEDLINE  
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[PMID]:29251849
[Au] Autor:Kumar S; Priya E; Singh Solanki D; Sharma R; Gehlot P; Pathak R; Singh SK
[Ti] Título:Occurrence and characterization of hitherto unknown Streptomyces species in semi-arid soils.
[So] Source:J Environ Biol;37(5):927-36, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Streptomyces the predominant genus of Actinobacteria and plays an important role in the recycling of soil organic matter and production of important secondary metabolites. The occurrence and diversity assessment of Streptomyces species revealed alkaline and poor nutrient status of soils of semi-arid region of Jodhpur, Rajasthan. The morphological and biochemical characterization of 21 Streptomyces isolates facilitated Genus level identification but were insufficient to designate species. Species designation based on 16S rRNA gene delineated 21 isolates into 14 Streptomyces species. Upon BLAST search, the test isolates exhibited 98 to 100% identities with that of the best aligned sequences of the NCBI database. The GC content of 16S rRNA gene sequences of all the Streptomyces isolates tested ranged from 59.03% to 60.94%. The multiple sequence alignment of all the 21 Streptomyces isolates generated a phylogram with high bootstrap values indicating reliable grouping of isolates based on nucleotide sequence variations by way of insertion, deletion and substitutions and 16S rRNA length polymorphism. Some of the Streptomyces species molecularly identified under present study are reported for the first time from semi-arid region of Jodhpur.
[Mh] Termos MeSH primário: Streptomyces/genética
Streptomyces/isolamento & purificação
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Índia
Técnicas de Amplificação de Ácido Nucleico
Filogenia
Polimorfismo Genético
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Microbiologia do Solo
Especificidade da Espécie
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  6 / 24062 MEDLINE  
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[PMID]:28745426
[Au] Autor:Tomada S; Sonego P; Moretto M; Engelen K; Pertot I; Perazzolli M; Puopolo G
[Ad] Endereço:Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all'Adige, Italy.
[Ti] Título:Dual RNA-Seq of Lysobacter capsici AZ78 - Phytophthora infestans interaction shows the implementation of attack strategies by the bacterium and unsuccessful oomycete defense responses.
[So] Source:Environ Microbiol;19(10):4113-4125, 2017 Oct.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biological interactions in the microbial communities of the rhizosphere continuously shape the gene expression patterns of each individual microorganism. A dual RNA-Seq approach was applied to obtain a comprehensive overview of the molecular mechanisms activated during the interaction between the biocontrol rhizobacterium Lysobacter capsici AZ78 and the soilborne phytopathogenic oomycete Phytophthora infestans. The RNA-Seq transcriptional profile of L. capsici AZ78 was characterized by up-regulation of genes concerned in the biogenesis of type 4 pilus and lytic enzymes, involved, respectively, in host colonization and subsequent attack of the P. infestans cell wall. The activation of detoxification processes allowed L. capsici AZ78 to overcome the attempted defense processes of P. infestans. Moreover, the genes involved in antibiotic biosynthesis were up-regulated in L. capsici AZ78 and caused cell death in P. infestans, with the activation of putative apoptotic processes. The consequences of P. infestans cell death resulted in the down-regulation of primary metabolic pathways, such as carbohydrates, nucleic acids and protein metabolisms. Overall, the mechanism of action of L. capsici AZ78 was related to parasitism and predatory activities that cause the death of P. infestans.
[Mh] Termos MeSH primário: Agentes de Controle Biológico
Lysobacter/genética
Lysobacter/patogenicidade
Phytophthora infestans/genética
Phytophthora infestans/microbiologia
RNA Bacteriano/genética
RNA de Protozoário/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Regulação da Expressão Gênica/genética
Doenças das Plantas/parasitologia
Raízes de Plantas/microbiologia
Raízes de Plantas/parasitologia
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Control Agents); 0 (RNA, Bacterial); 0 (RNA, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13861


  7 / 24062 MEDLINE  
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[PMID]:29257360
[Au] Autor:Abid I; Mujamammi R; Alkahtani MDF
[Ti] Título:Antimicrobial activity and molecular identification of Streptomyces strains isolated from Saudi Arabia.
[So] Source:J Environ Biol;37(6):1225-30, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Actinomycetes are group of Gram-positive bacteria with high GC-content in their DNA. They are extremely useful for the pharmaceutical industry due to their seemingly unlimited capacity to produce secondary metabolites with diverse biological activities and chemical structure. The genus Streptomyces constitutes 50% of the total population of soil actinomycetes and about 75% of commercially and medicinally useful antibiotics that have been derived from this genus. The present study aimed in isolation of bioactive compound showing antimicrobial activities from soil Streptomyces, previously isolated and morphologically characterized from Jazan in Saudi Arabia. Six potent Streptomyces strains: JS3, JS4, JS6, JD7, JA8 and JA10 were chosen for antimicrobial activity screening against 5 human pathogenic bacteria and 5 phytopathogenic fungi before molecular identification was done. For antibacterial activity, the results showed that inhibition zones were found to range between 3.25-26.875 mm diameters, while for antifungal activity, it ranged between 13.3-40 mm diameters. The entire sequence of the 16S rDNA was determined for the strains JS6, JD7, JA8 and JA10 and deposited in the GenBank. Future studies of actinomycetes isolated from the Kingdom of Saudi Arabia's soils will assist in the discovery of new compounds that would be of industrial, pharmaceutical and agricultural importance.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
DNA Bacteriano/genética
Avaliação Pré-Clínica de Medicamentos
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Arábia Saudita
Microbiologia do Solo
Streptomyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  8 / 24062 MEDLINE  
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[PMID]:29369557
[Au] Autor:Belova IV; Tochilina AG; Solovyeva IV; Efimov EI; Gorlova IS; Ivanova TP; Zhirnov VA
[Ti] Título:[Lactobacillus fermentum 90 TC-4 taxonomic status confirmation using whole genome sequencing and MALDI TOF mass spectrum].
[So] Source:Genetika;52(9):1021-8, 2016 Sep.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:With the use of whole genome sequencing, the taxonomic status of Lactobacillus fermentum 90 TC-4 strain from Russian collections were studied. Complex analysis of phenotypical and genetic properties was conducted using phenotypic and molecular genetic methods. The main characteristics of the genome and biochemical activity profile of the strain were determined. A comparative analysis of the mass spectrum of ribosomal proteins of the strain, its biochemical properties, a fragment of 16S rRNA gene sequencing, and the entire genome revealed that the present strain belongs to the species L. fermentum, confirming its taxonomic status in accordance with modern taxonomy.
[Mh] Termos MeSH primário: Lactobacillus fermentum
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Sequenciamento Completo do Genoma
[Mh] Termos MeSH secundário: Lactobacillus fermentum/classificação
Lactobacillus fermentum/genética
Lactobacillus fermentum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  9 / 24062 MEDLINE  
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[PMID]:28454900
[Au] Autor:Wei ZG; Zhang SW
[Ad] Endereço:Key Laboratory of Information Fusion Technology of Ministry of Education, College of Automation, Northwestern Polytechnical University, Xi'an 710072, China. Electronic address: david_nwpu@163.com.
[Ti] Título:DBH: A de Bruijn graph-based heuristic method for clustering large-scale 16S rRNA sequences into OTUs.
[So] Source:J Theor Biol;425:80-87, 2017 Jul 21.
[Is] ISSN:1095-8541
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent sequencing revolution driven by high-throughput technologies has led to rapid accumulation of 16S rRNA sequences for microbial communities. Clustering short sequences into operational taxonomic units (OTUs) is an initial crucial process in analyzing metagenomic data. Although many heuristic methods have been proposed for OTU inferences with low computational complexity, they just select one sequence as the seed for each cluster and the results are sensitive to the selected sequences that represent the clusters. To address this issue, we present a de Bruijn graph-based heuristic clustering method (DBH) for clustering massive 16S rRNA sequences into OTUs by introducing a novel seed selection strategy and greedy clustering approach. Compared with existing widely used methods on several simulated and real-life metagenomic datasets, the results show that DBH has higher clustering performance and low memory usage, facilitating the overestimation of OTUs number. DBH is more effective to handle large-scale metagenomic datasets. The DBH software can be freely downloaded from https://github.com/nwpu134/DBH.git for academic users.
[Mh] Termos MeSH primário: Heurística
Metagenômica/métodos
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Algoritmos
Análise por Conglomerados
Biologia Computacional/métodos
Microbioma Gastrointestinal/genética
Seres Humanos
Filogenia
RNA Bacteriano/genética
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  10 / 24062 MEDLINE  
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[PMID]:28455137
[Au] Autor:Ben Mouhoub R; El May A; Cheraief I; Landoulsi A
[Ad] Endereço:Biochemistry and Molecular Biology, Code UR13ES34 Research Unit, Faculty of Sciences of Bizerte, Zarzouna 7021, Carthage University, Tunisia. Electronic address: ramlabenmouhoub@gmail.com.
[Ti] Título:Influence of static magnetic field exposure on fatty acid composition in Salmonella Hadar.
[So] Source:Microb Pathog;108:13-20, 2017 Jul.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We have been interested, in this work, to investigate the effect of the exposure to static magnetic field at 200 mT (SMF) on the fatty acid (FA) composition of Salmonella enterica subsp Enterica serovar Hadar isolate 287: effects on the proportion of saturated and unsaturated fatty acids (SFAs, UFAs), cyclopropane fatty acids (CFAs) and hydroxy fatty acids after exposure to the static magnetic field at 200 mT (SMF). Analysis with Gas Chromatography-Mass Spectrometry (GC-MS) of total lipid showed that the proportion of the most fatty acids was clearly affected. The comparison of UFAs/SFAs ratio in exposed bacteria and controls showed a diminution after 3 and 6 h of exposure. This ration reached a balance after 9 h of treatment with SMF. So we can conclude that S. Hadar tries to adapt to magnetic stress by changing the proportions of SFAs and UFAs over time to maintain an equilibrium after 9 h of exposure, thus to maintain the inner membranes fluidity. Also, a decrease in the proportion of hydroxy FAs was observed after 6 h but an increase of this proportion after 9 h of exposure. Concerning CFAs, its proportion raised after 6 h of exposure to the SMF but it decreased after 9 h of exposure. These results are strongly correlated with those of cfa (cyclopropane fatty acid synthase) gene expression which showed a decrease of its expression after 9 h of exposure.
[Mh] Termos MeSH primário: Ácidos Graxos/análise
Campos Magnéticos
Salmonella enterica/metabolismo
Salmonella enterica/efeitos da radiação
[Mh] Termos MeSH secundário: Ciclopropanos/análise
Ciclopropanos/química
Ácidos Graxos/química
Ácidos Graxos/genética
Ácidos Graxos Insaturados/análise
Ácidos Graxos Insaturados/química
Ácidos Graxos Insaturados/genética
Cromatografia Gasosa-Espectrometria de Massas
Regulação Bacteriana da Expressão Gênica/efeitos da radiação
Fluidez de Membrana/efeitos da radiação
Lipídeos de Membrana
Metiltransferases/genética
Metiltransferases/efeitos da radiação
RNA Bacteriano/análise
RNA Ribossômico 16S/genética
Salmonella enterica/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopropanes); 0 (Fatty Acids); 0 (Fatty Acids, Unsaturated); 0 (Membrane Lipids); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (cyclopropane fatty acids); EC 2.1.1.- (Methyltransferases); EC 2.1.1.- (cyclopropane synthetase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE



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