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[PMID]:29293661
[Au] Autor:Monson EA; Crosse KM; Das M; Helbig KJ
[Ad] Endereço:Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, Victoria.
[Ti] Título:Lipid droplet density alters the early innate immune response to viral infection.
[So] Source:PLoS One;13(1):e0190597, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cellular localisation of many innate signalling events following viral infection has yet to be elucidated, however there has been a few cases in which membranes of certain cellular organelles have acted as platforms to these events. Of these, lipid droplets (LDs) have recently been identified as signalling platforms for innate TLR7 and 9 signalling. Despite their wide range of similar roles in various metabolic pathways, LDs have been overlooked as potential platforms for antiviral innate signalling events. This study established an in vitro model to evaluate the efficiency of the early innate immune response in cells with reduced LD content to the viral mimics, dsDNA and dsRNA, and Sendai viral infection. Using RT-qPCR, the expression of IFN-ß and IFN-λ was quantified following stimulation along with the expression of specific ISGs. Luciferase based assays evaluated the combined expression of ISRE-promoter driven ISGs under IFN-ß stimulation. Cellular LD content did not alter the entry of fluorescently labelled viral mimics into cells, but significantly decreased the ability of both Huh-7 and HeLa cells to produce type I and III IFN, as well as downstream ISG expression, indicative of an impeded innate immune response. This observation was also seen during Sendai virus infection of HeLa cells, where both control and LD reduced cells replicated the virus to the same level, but a significantly impaired type I and III IFN response was observed in the LD reduced cells. In addition to altered IFN production, cells with reduced LD content exhibited decreased expression of specific antiviral ISGs: Viperin, IFIT-1 and OAS-1 under IFN-ß stimulation; However the overall induction of the ISRE-promoter was not effected. This study implicates a role for LDs in an efficient early innate host response to viral infection and future work will endeavour to determine the precise role these important organelles play in induction of an antiviral response.
[Mh] Termos MeSH primário: Imunidade Inata
Gotículas Lipídicas/metabolismo
Viroses/imunologia
[Mh] Termos MeSH secundário: Western Blotting
Linhagem Celular
Meios de Cultura
Seres Humanos
Interferon Tipo I/imunologia
Ácidos Nucleicos/metabolismo
RNA de Cadeia Dupla/imunologia
Reação em Cadeia da Polimerase em Tempo Real
Vírus Sendai/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (Interferon Type I); 0 (Nucleic Acids); 0 (RNA, Double-Stranded)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190597


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[PMID]:28467379
[Au] Autor:Zhong J; Cheng CY; Gao BD; Zhou Q; Zhu HJ
[Ad] Endereço:Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha 410128, China. wzzhtx@sina.com.
[Ti] Título:Mycoviruses in the Plant Pathogen Ustilaginoidea virens Are Not Correlated with the Genetic Backgrounds of Its Hosts.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:, the causal agent of rice false smut, is one of the most devastating grain diseases that causes loss of yield in most rice-growing areas worldwide. In this study, we performed a dsRNA screen to isolate mycoviruses from 35 strains. The results revealed that 34 of the tested isolates were infected by various dsRNA elements, displaying highly viral diversity and mixed infections. We characterized a 5.3 kbp dsRNA from a typical isolate containing dsRNA segments with sizes ranging from 0.5 to 5.3 kbp. Sequence analysis of its genomic properties indicated that it is a novel victorivirus, named RNA virus 5 (UvRV5), that belongs to the family . RT-PCR detection was performed and indicated that not all the dsRNA bands that were 5.3 kbp in size contained UvRV5. Moreover, the genetic relatedness of all the strains was estimated according to phylogenetic analysis of the partial intergenic spacer region (IGS) sequences. However, concordance was not found between the dsRNA profiles and the IGS-based genetic relatedness of their host fungi.
[Mh] Termos MeSH primário: Micovírus/genética
Interações Hospedeiro-Patógeno/genética
Hypocreales/genética
Hypocreales/virologia
Oryza/microbiologia
Doenças das Plantas/microbiologia
Totiviridae/genética
[Mh] Termos MeSH secundário: Patrimônio Genético
Genoma Fúngico
Genoma Viral
Filogenia
RNA de Cadeia Dupla/isolamento & purificação
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:29339143
[Au] Autor:Komisarczuk AZ; Kongshaug H; Nilsen F
[Ad] Endereço:Sea Lice Research Centre, Department of Biology, University of Bergen, Thormøhlensgate 55, 5008, Bergen, Norway. Electronic address: Anna.Komisarczuk@uib.no.
[Ti] Título:Gene silencing reveals multiple functions of Na /K -ATPase in the salmon louse (Lepeophtheirus salmonis).
[So] Source:Exp Parasitol;185:79-91, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Na /K -ATPase has a key function in a variety of physiological processes including membrane excitability, osmoregulation, regulation of cell volume, and transport of nutrients. While knowledge about Na /K -ATPase function in osmoregulation in crustaceans is extensive, the role of this enzyme in other physiological and developmental processes is scarce. Here, we report characterization, transcriptional distribution and likely functions of the newly identified L. salmonis Na /K -ATPase (LsalNa /K -ATPase) α subunit in various developmental stages. The complete mRNA sequence was identified, with 3003 bp open reading frame encoding a putative protein of 1001 amino acids. Putative protein sequence of LsalNa /K -ATPase revealed all typical features of Na /K -ATPase and demonstrated high sequence identity to other invertebrate and vertebrate species. Quantitative RT-PCR analysis revealed higher LsalNa /K -ATPase transcript level in free-living stages in comparison to parasitic stages. In situ hybridization analysis of copepodids and adult lice revealed LsalNa /K -ATPase transcript localization in a wide variety of tissues such as nervous system, intestine, reproductive system, and subcuticular and glandular tissue. RNAi mediated knock-down of LsalNa /K -ATPase caused locomotion impairment, and affected reproduction and feeding. Morphological analysis of dsRNA treated animals revealed muscle degeneration in larval stages, severe changes in the oocyte formation and maturation in females and abnormalities in tegmental glands. Thus, the study represents an important foundation for further functional investigation and identification of physiological pathways in which Na /K -ATPase is directly or indirectly involved.
[Mh] Termos MeSH primário: Copépodes/enzimologia
Inativação Gênica
ATPase Trocadora de Sódio-Potássio/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Copépodes/genética
Copépodes/crescimento & desenvolvimento
Copépodes/fisiologia
DNA Complementar/química
Ectoparasitoses/parasitologia
Ectoparasitoses/veterinária
Feminino
Doenças dos Peixes/parasitologia
Pesqueiros
Regulação Enzimológica da Expressão Gênica
Técnicas de Silenciamento de Genes
Hibridização In Situ
Masculino
Fases de Leitura Aberta/genética
Filogenia
Interferência de RNA
RNA de Cadeia Dupla
RNA Mensageiro/química
Reação em Cadeia da Polimerase em Tempo Real
Salmo salar/parasitologia
Água do Mar
Alinhamento de Sequência
ATPase Trocadora de Sódio-Potássio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Double-Stranded); 0 (RNA, Messenger); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29232690
[Au] Autor:Anandanarayanan A; Raina OK; Lalrinkima H; Rialch A; Sankar M; Varghese A
[Ad] Endereço:Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, UP-India.
[Ti] Título:RNA interference in Fasciola gigantica: Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke.
[So] Source:PLoS Negl Trop Dis;11(12):e0006109, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fasciolosis caused by Fasciola gigantica is a neglected tropical disease but a constraint on the growth and productivity of cattle, buffaloes and sheep in the tropical countries of Asia and Africa. Resistance to commonly used anthelmintics in Fasciola has increased the need to search for alternative therapeutic targets. RNA interference is the current tool of choice in the search for such targets in Fasciola. The susceptibility of juvenile Fasciola hepatica to double stranded (ds) RNA induced RNAi has been established but in F. gigantica a single preliminary report on RNAi induced mRNA transcript knockdown is available. Here we optimized conditions for RNAi in the liver fluke F.gigantica targeting six genes including superoxide dismutase (SOD), σ class of glutathione-s-transferase (GST), cathepsin (Cat) L1-D, Cat B1, Cat B2 and Cat B3 that showed robust transcriptional silencing of the targets following exposure of the newly excysted juveniles (NEJs) to long (170-223 nt) dsRNA. Knockdown was shown to be concentration dependent with significant mRNA transcript suppression occurring at 5 ng / µl that showed further suppression with the increase in the dsRNA concentration. The dsRNA induced persistent silencing of the mRNA transcript of SOD and σGST up to 15 days of observation. Delivery of the long dsRNA and siRNA to the newly excysted juveniles by soaking method was found to be efficient by tracking the uptake and diffusion of Cy3 labelled siRNA and long dsRNA in the flukes. Off-target effects of dsRNA trigger on some of the non-target genes were detected in the present investigation on RNAi in F. gigantica. The dsRNA induced superoxide dismutase protein suppression while impact of RNAi on other target proteins was not studied. There is no in vitro culture system for prolonged survival of the F. gigantica and in the present study in vitro maintenance of the NEJs is reported for a period of 3 weeks. The present study is the first attempt on optimization of RNAi protocols in F. gigantica where long dsRNA allowed for an efficient and persistent gene silencing, opening prospects for functional validation of putative vaccine and therapeutic targets in this neglected parasite.
[Mh] Termos MeSH primário: Fasciola hepatica/genética
Fasciolíase/parasitologia
Interferência de RNA
[Mh] Termos MeSH secundário: Animais
Proteínas de Helminto/genética
RNA de Cadeia Dupla/genética
RNA Mensageiro/genética
RNA Interferente Pequeno/genética
Superóxido Dismutase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Helminth Proteins); 0 (RNA, Double-Stranded); 0 (RNA, Messenger); 0 (RNA, Small Interfering); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006109


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[PMID]:29325850
[Au] Autor:Nejat Dehkordi M; Akerman B
[Ad] Endereço:Department of Basic Science, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran; Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. Electronic address: m.nejat@iaushk.ac.ir.
[Ti] Título:Interaction of DNA with water soluble complex of Nickle and formation of DNA cross-links.
[So] Source:Chem Biol Interact;282:55-62, 2018 Feb 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Interaction of double stranded DNA with bulky and hydrophobic Salen type Schiff base complex: [N, N' Bis [3- tert-butyl-5-[triphenyl-phosphonium - methyl] - salicylidene] 1,2 ethylene-diamine nickel(III) acetate (refer to Ni Salen complex) was extensively investigated using the spectroscopic techniques and gel electrophoresis. Absorption titration experiment showed the hypochromic effect and the significant red shift of the complex absorption. In competition experiments with ethidium bromide (EB), Ni Salen complex exhibited non-competitive binding at high concentrations. UV-vis absorption and fluorescence emission data agreed on a binding constant of (1.64 ±â€¯0.01) µM , thereby showing the strong interaction of the complex with DNA; also, a binding site size of 2.33 ±â€¯0.01 base pairs per complex was achieved. Thermal denaturation experiment showed that T of calf thymus-DNA was increased by approximately 10 °C at a molar ratio of the dye/base of 0.2. The CD spectra of DNA exhibited an increase in both positive and negative peaks without any shift in the position of bands upon addition of the complex. The amplitude of the LD spectra of DNA was decreased in the presence of the complex. Reduced linear dichroism (LD ) revealed that the transition moment of complex was parallel to the DNA helix axis. Gel electrophoresis experiments confirmed that Ni Salen complex had no nuclease/DNA cleaving activity; also, DNA-DNA cross links were formed at high concentrations of complex, leading to the aggregation of DNA.
[Mh] Termos MeSH primário: DNA/química
Níquel/química
RNA de Cadeia Dupla/química
Água/química
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Bovinos
Eletroforese em Gel de Ágar/métodos
Etídio/química
Etilenodiaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (RNA, Double-Stranded); 059QF0KO0R (Water); 7OV03QG267 (Nickel); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); 94-93-9 (disalicylaldehyde ethylenediamine); EN464416SI (Ethidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


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[PMID]:29342168
[Au] Autor:Sanscrainte ND; Arimoto H; Waits CM; Li LY; Johnson D; Geden C; Becnel JJ; Estep AS
[Ad] Endereço:USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, Gainesville, Florida, United States of America.
[Ti] Título:Reduction in Musca domestica fecundity by dsRNA-mediated gene knockdown.
[So] Source:PLoS One;13(1):e0187353, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:House flies (Musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the U.S. Non-target effects and widespread resistance challenge the efficacy of traditional chemical control. Double stranded RNA (dsRNA) has been suggested as a biopesticide for M. domestica but a phenotypic response due to the induction of the RNAi pathway has not been demonstrated in adults. In this study female house flies were injected with dsRNA targeting actin-5C or ribosomal protein (RP) transcripts RPL26 and RPS6. Ovaries showed highly reduced provisioning and clutch reductions of 94-99% in RP dsRNA treated flies but not in actin-5C or GFP treated flies. Gene expression levels were significantly and specifically reduced in dsRNA injected groups but remained unchanged in the control dsGFP treated group. Furthermore, injections with an Aedes aegypti conspecific dsRNA designed against RPS6 did not impact fecundity, demonstrating species specificity of the RNAi response. Analysis of M. domestica tissues following RPS6 dsRNA injection showed significant reduction of transcript levels in the head, thorax, and abdomen but increased expression in ovarian tissues. This study demonstrates that exogenous dsRNA is specifically effective and has potential efficacy as a highly specific biocontrol intervention in adult house flies. Further work is required to develop effective methods for delivery of dsRNA to adult flies.
[Mh] Termos MeSH primário: Fertilidade/genética
Técnicas de Silenciamento de Genes
Moscas Domésticas/fisiologia
RNA de Cadeia Dupla/genética
[Mh] Termos MeSH secundário: Animais
Feminino
Proteínas de Fluorescência Verde/genética
Ovário/anatomia & histologia
Oviposição
Interferência de RNA
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187353


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[PMID]:29211987
[Au] Autor:Liu Y; Lilley DMJ
[Ad] Endereço:Cancer Research UK Nucleic Acid Structure Research Group, MSI/WTB Complex, The University of Dundee, Dundee, United Kingdom.
[Ti] Título:Crystal Structures of Cyanine Fluorophores Stacked onto the End of Double-Stranded RNA.
[So] Source:Biophys J;113(11):2336-2343, 2017 Dec 05.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The indodicarbocyanine fluorophores Cy3 and Cy5 are extensively used as donor-acceptor pairs in fluorescence resonance energy transfer experiments, especially those involving single molecules. When terminally attached to double-stranded nucleic acids via the 5' phosphate group these fluorophores stack onto the ends of the molecule. Knowledge of the positions of the fluorophores is critical to the interpretation of fluorescence resonance energy transfer data. The positions have been demonstrated for double-stranded (ds) DNA using NMR spectroscopy. Here, we have used x-ray crystallography to analyze the location of Cy3 and Cy5 on dsRNA, using complexes of an RNA stem-loop bound to L5 protein determined at 2.4 Å resolution. This confirms the tendency of both fluorophores to stack on the free end of RNA, with the long axis of the fluorophores approximately parallel to that of the terminal basepair. However, the manner of interaction of both Cy3 and Cy5 with the terminus of the dsRNA is significantly different from that deduced for dsDNA using NMR. The fluorophores are stacked on the terminal basepair such that their indole nitrogen atoms lie on the major groove side, and thus their pendant methyl groups are on the minor groove side.
[Mh] Termos MeSH primário: Carbocianinas/química
Corantes Fluorescentes/química
RNA de Cadeia Dupla/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Pareamento de Bases
Cristalografia por Raios X
Modelos Moleculares
Conformação Proteica
RNA de Cadeia Dupla/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (RNA, Double-Stranded)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180120
[Lr] Data última revisão:
180120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


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[PMID]:29251589
[Au] Autor:Ghabrial SA; Castón JR; Coutts RHA; Hillman BI; Jiang D; Kim DH; Moriyama H; Ictv Report Consortium
[Ad] Endereço:1​Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
[Ti] Título:ICTV Virus Taxonomy Profile: Chrysoviridae.
[So] Source:J Gen Virol;99(1):19-20, 2018 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Chrysoviridae is a family of small, isometric, non-enveloped viruses (40 nm in diameter) with segmented dsRNA genomes (typically four segments). The genome segments are individually encapsidated and together comprise 11.5-12.8 kbp. The single genus Chrysovirus includes nine species. Chrysoviruses lack an extracellular phase to their life cycle; they are transmitted via intracellular routes within an individual during hyphal growth, in asexual or sexual spores, or between individuals via hyphal anastomosis. There are no known natural vectors for chrysoviruses. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Chrysoviridae, which is available at www.ictv.global/report/chrysoviridae.
[Mh] Termos MeSH primário: Genoma Viral
Filogenia
Vírus de RNA/genética
RNA de Cadeia Dupla/genética
RNA Viral/genética
Vírion/genética
[Mh] Termos MeSH secundário: Ascomicetos/virologia
Basidiomycota/virologia
Hifas/virologia
Vírus de RNA/classificação
Vírus de RNA/ultraestrutura
Esporos Fúngicos/virologia
Terminologia como Assunto
Vírion/ultraestrutura
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 0 (RNA, Viral)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000994


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[PMID]:28468824
[Au] Autor:Talwar T; Vidhyasagar V; Qing J; Guo M; Kariem A; Lu Y; Singh RS; Lukong KE; Wu Y
[Ad] Endereço:From the Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada.
[Ti] Título:The DEAD-box protein DDX43 (HAGE) is a dual RNA-DNA helicase and has a K-homology domain required for full nucleic acid unwinding activity.
[So] Source:J Biol Chem;292(25):10429-10443, 2017 06 23.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The K-homology (KH) domain is a nucleic acid-binding domain present in many proteins but has not been reported in helicases. DDX43, also known as ( elicase ntigen ne), is a member of the DEAD-box protein family. It contains a helicase core domain in its C terminus and a potential KH domain in its N terminus. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy. Despite its potential as a therapeutic target, little is known about its activities. Here, we purified recombinant DDX43 protein to near homogeneity and found that it exists as a monomer in solution. Biochemical assays demonstrated that it is an ATP-dependent RNA and DNA helicase. Although DDX43 was active on duplex RNA regardless of the orientation of the single-stranded RNA tail, it preferred a 5' to 3' polarity on RNA and a 3' to 5' direction on DNA. Truncation mutations and site-directed mutagenesis confirmed that the KH domain in DDX43 is responsible for nucleic acid binding. Compared with the activity of the full-length protein, the C-terminal helicase domain had no unwinding activity on RNA substrates and had significantly reduced unwinding activity on DNA. Moreover, the full-length DDX43 protein, with single amino acid change in the KH domain, had reduced unwinding and binding activates on RNA and DNA substrates. Our results demonstrate that DDX43 is a dual helicase and the KH domain is required for its full unwinding activity.
[Mh] Termos MeSH primário: RNA Helicases DEAD-box/química
Proteínas de Neoplasias/química
RNA de Cadeia Dupla/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Seres Humanos
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Domínios Proteicos
RNA de Cadeia Dupla/metabolismo
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Neoplasm Proteins); 0 (RNA, Double-Stranded); EC 3.6.1.- (DDX43 protein, human); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.774950


  10 / 6542 MEDLINE  
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[PMID]:27770578
[Au] Autor:Perkin LC; Elpidina EN; Oppert B
[Ad] Endereço:USDA, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Avenue, Manhattan, KS, USA.
[Ti] Título:RNA interference and dietary inhibitors induce a similar compensation response in Tribolium castaneum larvae.
[So] Source:Insect Mol Biol;26(1):35-45, 2017 02.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tribolium castaneum is a major agriculture pest damaging stored grains and cereal products. The T. castaneum genome contains 26 cysteine peptidase genes, mostly cathepsins L and B, and seven have a major role in digestion. We targeted the expression of the most highly expressed cathepsin L gene on chromosome 10, TC011001, by RNA interference (RNAi), using double-stranded RNA (dsRNA) constructs of different regions of the gene (3', middle, 5' and entire coding regions). RNA sequencing and quantitation (RNA-seq) was used to evaluate knockdown and specificity amongst the treatments. Overall, target gene expression decreased in all treatment groups, but was more severe and specific in dsRNA targeting the 3' and entire coding regions, encoding the proteolytic active site in the enzyme. Additional cysteine cathepsin genes also were down-regulated (off-target effects), but some were up-regulated in response to RNAi treatment. Notably, some serine peptidase genes were increased in expression, especially in dsRNA targeting 5' and middle regions, and the response was similar to the effects of dietary cysteine protease inhibitors. We manually annotated these serine peptidase genes to gain insight into function and relevance to the RNAi study. The data indicate that T. castaneum larvae compensate for the loss of digestive peptidase activity in the larval gut, regardless of the mechanism of disruption.
[Mh] Termos MeSH primário: Catepsina L/genética
Interferência de RNA
Tribolium/genética
[Mh] Termos MeSH secundário: Animais
Catepsina L/metabolismo
Cisteína Proteases/metabolismo
Larva/metabolismo
RNA de Cadeia Dupla
Serina Proteases/metabolismo
Transcriptoma
Tribolium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Double-Stranded); EC 3.4.- (Cysteine Proteases); EC 3.4.- (Serine Proteases); EC 3.4.22.15 (Cathepsin L)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12269



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