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[PMID]:29422501
[Au] Autor:Collopy LC; Ware TL; Goncalves T; Í Kongsstovu S; Yang Q; Amelina H; Pinder C; Alenazi A; Moiseeva V; Pearson SR; Armstrong CA; Tomita K
[Ad] Endereço:Chromosome Maintenance Group, UCL Cancer Institute, University College London, London, WC1E 6DD, UK.
[Ti] Título:LARP7 family proteins have conserved function in telomerase assembly.
[So] Source:Nat Commun;9(1):557, 2018 02 08.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Understanding the intricacies of telomerase regulation is crucial due to the potential health benefits of modifying its activity. Telomerase is composed of an RNA component and reverse transcriptase. However, additional factors required during biogenesis vary between species. Here we have identified fission yeast Lar7 as a member of the conserved LARP7 family, which includes the Tetrahymena telomerase-binding protein p65 and human LARP7. We show that Lar7 has conserved RNA-recognition motifs, which bind telomerase RNA to protect it from exosomal degradation. In addition, Lar7 is required to stabilise the association of telomerase RNA with the protective complex LSm2-8, and telomerase reverse transcriptase. Lar7 remains a component of the mature telomerase complex and is required for telomerase localisation to the telomere. Collectively, we demonstrate that Lar7 is a crucial player in fission yeast telomerase biogenesis, similarly to p65 in Tetrahymena, and highlight the LARP7 family as a conserved factor in telomere maintenance.
[Mh] Termos MeSH primário: Proteínas Nucleares/genética
Fosfoproteínas/genética
Proteínas de Protozoários/genética
RNA Fúngico/genética
DNA Polimerase Dirigida por RNA/genética
RNA/genética
Ribonucleoproteínas/genética
Schizosaccharomyces/genética
Telomerase/genética
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência Conservada
Expressão Gênica
Seres Humanos
Isoenzimas/genética
Isoenzimas/metabolismo
Proteínas Nucleares/metabolismo
Fosfoproteínas/metabolismo
Ligação Proteica
Proteínas de Protozoários/metabolismo
RNA/metabolismo
Estabilidade de RNA
RNA Fúngico/metabolismo
DNA Polimerase Dirigida por RNA/metabolismo
Ribonucleoproteínas/metabolismo
Schizosaccharomyces/metabolismo
Telomerase/metabolismo
Telômero/química
Telômero/ultraestrutura
Tetrahymena thermophila/genética
Tetrahymena thermophila/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Larp7 protein, human); 0 (Nuclear Proteins); 0 (Pdd1 protein, Tetrahymena); 0 (Phosphoproteins); 0 (Protozoan Proteins); 0 (RNA, Fungal); 0 (Ribonucleoproteins); 0 (Ter1 telomerase subunit, S pombe); 63231-63-0 (RNA); EC 2.7.7.49 (RNA-Directed DNA Polymerase); EC 2.7.7.49 (Telomerase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02296-4


  2 / 7334 MEDLINE  
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[PMID]:29281624
[Au] Autor:Lafuente-Barquero J; Luke-Glaser S; Graf M; Silva S; Gómez-González B; Lockhart A; Lisby M; Aguilera A; Luke B
[Ad] Endereço:Andalusian Center for Molecular Biology and Regenerative Medicine-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Avda. Americo Vespucio 24, Seville, Spain.
[Ti] Título:The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage.
[So] Source:PLoS Genet;13(12):e1007136, 2017 12.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity. We demonstrate that Mph1, the yeast homolog of Fanconi anemia protein M (FANCM), is required for cell viability in the absence of RNase H enzymes. The integrity of the Mph1 helicase domain is crucial to prevent the accumulation of RNA-DNA hybrids and RNA-DNA hybrid-dependent DNA damage, as determined by Rad52 foci. Mph1 forms foci when RNA-DNA hybrids accumulate, e.g. in RNase H or THO-complex mutants and at short telomeres. Mph1, however is a double-edged sword, whose action at hybrids must be regulated by the Smc5/6 complex. This is underlined by the observation that simultaneous inactivation of RNase H2 and Smc5/6 results in Mph1-dependent synthetic lethality, which is likely due to an accumulation of toxic recombination intermediates. The data presented here support a model, where Mph1's helicase activity plays a crucial role in responding to persistent RNA-DNA hybrids.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/genética
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Dano ao DNA
RNA Fúngico/genética
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/metabolismo
DNA/metabolismo
Reparo do DNA
Replicação do DNA/genética
Replicação do DNA/fisiologia
RNA Helicases/metabolismo
RNA Fúngico/metabolismo
Ribonuclease H/genética
Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (RNA, Fungal); 0 (SMC5 protein, S cerevisiae); 0 (SMC6 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 9007-49-2 (DNA); EC 3.1.26.4 (Ribonuclease H); EC 3.6.1.- (MPH1 protein, S cerevisiae); EC 3.6.4.13 (DEAD-box RNA Helicases); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007136


  3 / 7334 MEDLINE  
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[PMID]:28461681
[Au] Autor:Wise JA; Nielsen O
[Ad] Endereço:Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106-4906 jaw17@case.edu.
[Ti] Título:Analysis of RNA Metabolism in Fission Yeast.
[So] Source:Cold Spring Harb Protoc;2017(5):pdb.top079798, 2017 May 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we focus on the biogenesis and function of messenger RNA (mRNA) in fission yeast cells. Following a general introduction that also briefly touches on other classes of RNA, we provide an overview of methods used to analyze mRNAs throughout their life cycles.
[Mh] Termos MeSH primário: RNA Fúngico/metabolismo
Schizosaccharomyces/genética
[Mh] Termos MeSH secundário: Biossíntese de Proteínas
Processamento de RNA
RNA Mensageiro/metabolismo
RNA Ribossômico/metabolismo
RNA Interferente Pequeno/metabolismo
RNA Nucleolar Pequeno/metabolismo
RNA de Transferência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Fungal); 0 (RNA, Messenger); 0 (RNA, Ribosomal); 0 (RNA, Small Interfering); 0 (RNA, Small Nucleolar); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.top079798


  4 / 7334 MEDLINE  
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[PMID]:28461655
[Au] Autor:Mata J; Wise JA
[Ad] Endereço:Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom jm593@cam.ac.uk.
[Ti] Título:4-Thiouridine Labeling to Analyze mRNA Turnover in .
[So] Source:Cold Spring Harb Protoc;2017(5):pdb.prot091645, 2017 May 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traditionally, the half-lives of mRNAs were measured after inhibition of transcription to allow decay of the preexisting population. The protocol presented here is a more recently developed strategy in which mRNA turnover is analyzed by measuring the decline in levels of newly synthesized RNA labeled with 4-thiouridine (4sU) during a brief pulse. After RNA extraction, the 4sU is biotinylated and the labeled species are purified using streptavidin beads. DNA microarrays can then be used to compare this population with total RNA, allowing half-lives to be calculated.
[Mh] Termos MeSH primário: Marcadores de Afinidade
RNA Fúngico/metabolismo
RNA Mensageiro/metabolismo
Schizosaccharomyces/metabolismo
Tiouridina
[Mh] Termos MeSH secundário: Antimetabólitos
Biotinilação
Meia-Vida
Indicadores e Reagentes
Análise de Sequência com Séries de Oligonucleotídeos
RNA Fúngico/biossíntese
RNA Mensageiro/análise
RNA Mensageiro/biossíntese
Estreptavidina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Affinity Labels); 0 (Antimetabolites); 0 (Indicators and Reagents); 0 (RNA, Fungal); 0 (RNA, Messenger); 13957-31-8 (Thiouridine); 9013-20-1 (Streptavidin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot091645


  5 / 7334 MEDLINE  
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[PMID]:25815561
[Au] Autor:Wang XC; Wu K; Chen H; Shao J; Zhang N; Chen X; Lan J; Liu C
[Ad] Endereço:a Institute of Medicinal Plant Development, Chinese Academy of Medical Science , Beijing , PR China.
[Ti] Título:The complete mitochondrial genome of the white-rot fungus Ganoderma meredithiae (Polyporales, Basidiomycota).
[So] Source:Mitochondrial DNA A DNA Mapp Seq Anal;27(6):4197-4198, 2016 Nov.
[Is] ISSN:2470-1408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Complete nucleotide sequence of the 78,447 bp mitochondrial genome of the white-rotting fungus Ganoderma meredithiae Adask. & Gilb. has been determined by next-generation sequencing technology. The circular molecule encodes a set of mitochondrial protein and RNA genes, including 15 conserved proteins, 29 tRNAs, large and small ribosomal RNAs, and 18 homing endonucleases, with a GC content of 26.14%. All structural genes are located on the same strand except trnW-CCA. Compared with previously sequenced mtDNAs of G. lucidum and G. sinense, the gene order of protein and rRNA genes among the three mitogenomes is highly conserved; however, the tRNA composition is slightly different. The mitochondrial genome of G. meredithiae will contribute to understanding the phylogeny and evolution of Ganoderma and Ganodermataceae, the group containing many species with high medicinal values.
[Mh] Termos MeSH primário: Ganoderma/genética
Genoma Mitocondrial/genética
[Mh] Termos MeSH secundário: DNA Mitocondrial/genética
Proteínas Fúngicas/genética
Proteínas Mitocondriais/genética
RNA/genética
RNA Fúngico/genética
RNA Ribossômico/genética
RNA de Transferência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Fungal Proteins); 0 (Mitochondrial Proteins); 0 (RNA, Fungal); 0 (RNA, Ribosomal); 0 (RNA, mitochondrial); 63231-63-0 (RNA); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150328
[St] Status:MEDLINE


  6 / 7334 MEDLINE  
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[PMID]:28449100
[Au] Autor:Villada JC; Brustolini OJB; Batista da Silveira W
[Ad] Endereço:Department of Microbiology, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
[Ti] Título:Integrated analysis of individual codon contribution to protein biosynthesis reveals a new approach to improving the basis of rational gene design.
[So] Source:DNA Res;24(4):419-434, 2017 Aug 01.
[Is] ISSN:1756-1663
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gene codon optimization may be impaired by the misinterpretation of frequency and optimality of codons. Although recent studies have revealed the effects of codon usage bias (CUB) on protein biosynthesis, an integrated perspective of the biological role of individual codons remains unknown. Unlike other previous studies, we show, through an integrated framework that attributes of codons such as frequency, optimality and positional dependency should be combined to unveil individual codon contribution for protein biosynthesis. We designed a codon quantification method for assessing CUB as a function of position within genes with a novel constraint: the relativity of position-dependent codon usage shaped by coding sequence length. Thus, we propose a new way of identifying the enrichment, depletion and non-uniform positional distribution of codons in different regions of yeast genes. We clustered codons that shared attributes of frequency and optimality. The cluster of non-optimal codons with rare occurrence displayed two remarkable characteristics: higher codon decoding time than frequent-non-optimal cluster and enrichment at the 5'-end region, where optimal codons with the highest frequency are depleted. Interestingly, frequent codons with non-optimal adaptation to tRNAs are uniformly distributed in the Saccharomyces cerevisiae genes, suggesting their determinant role as a speed regulator in protein elongation.
[Mh] Termos MeSH primário: Códon
Biossíntese de Proteínas
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: RNA Fúngico/metabolismo
RNA de Transferência/metabolismo
Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (RNA, Fungal); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/dnares/dsx014


  7 / 7334 MEDLINE  
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[PMID]:28450395
[Au] Autor:Zhang Q; Meng X; Li D; Chen S; Luo J; Zhu L; Singer RH; Gu W
[Ad] Endereço:From the Department of Pathophysiology, Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, Guangdong Province 515031, China and.
[Ti] Título:Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region of mRNA represses localized translation of in yeast cells.
[So] Source:J Biol Chem;292(23):9787-9800, 2017 06 09.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Local translation of specific mRNAs is regulated by dynamic changes in their subcellular localization, and these changes are due to complex mechanisms controlling cytoplasmic mRNA transport. The budding yeast is well suited to studying these mechanisms because many of its transcripts are transported from the mother cell to the budding daughter cell. Here, we investigated the translational control of mRNA after transport and localization. We show that although transcripts were translated after they reached the bud tip, some mRNAs were bound by the RNA-binding protein Puf6 and were non-polysomal. We also found that the DEAD-box helicase Dhh1 complexed with the untranslated mRNA and Puf6. Loss of Dhh1 affected local translation of mRNA and resulted in delocalization of transcript in the bud. Forcibly shifting the non-polysomal mRNA into polysomes was associated with Dhh1 dissociation. We further demonstrated that Dhh1 is not recruited to mRNA co-transcriptionally, suggesting that it could bind to mRNA within the cytoplasm. Of note, Dhh1 bound to the 5'-UTR of mRNA and inhibited its translation These results suggest that after localization to the bud tip, a portion of the localized mRNA becomes translationally inactive because of binding of Dhh1 and Puf6 to the 5'- and 3'-UTRs of mRNA.
[Mh] Termos MeSH primário: Regiões 5´ não Traduzidas/fisiologia
RNA Helicases DEAD-box/metabolismo
Biossíntese de Proteínas/fisiologia
RNA Fúngico/metabolismo
Proteínas Repressoras/biossíntese
Proteínas de Saccharomyces cerevisiae/biossíntese
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: RNA Helicases DEAD-box/genética
RNA Fúngico/genética
Proteínas de Ligação a RNA/genética
Proteínas de Ligação a RNA/metabolismo
Proteínas Repressoras/genética
Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (ASH1 protein, S cerevisiae); 0 (Puf6 protein, S cerevisiae); 0 (RNA, Fungal); 0 (RNA-Binding Proteins); 0 (Repressor Proteins); 0 (Saccharomyces cerevisiae Proteins); EC 3.6.1.- (DHH1 protein, S cerevisiae); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.776492


  8 / 7334 MEDLINE  
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[PMID]:29220656
[Au] Autor:Yurko N; Liu X; Yamazaki T; Hoque M; Tian B; Manley JL
[Ad] Endereço:Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
[Ti] Título:MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD.
[So] Source:Mol Cell;68(5):913-925.e3, 2017 Dec 07.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The RNA polymerase II largest subunit C-terminal domain consists of repeated YSPTSPS heptapeptides. The role of tyrosine-1 (Tyr1) remains incompletely understood, as, for example, mutating all Tyr1 residues to Phe (Y1F) is lethal in vertebrates but a related mutant has only a mild phenotype in S. pombe. Here we show that Y1F substitution in budding yeast resulted in a strong slow-growth phenotype. The Y1F strain was also hypersensitive to several different cellular stresses that involve MAP kinase signaling. These phenotypes were all linked to transcriptional changes, and we also identified genetic and biochemical interactions between Tyr1 and both transcription initiation and termination factors. Further studies uncovered defects related to MAP kinase I (Slt2) pathways, and we provide evidence that Slt2 phosphorylates Tyr1 in vitro and in vivo. Our study has thus identified Slt2 as a Tyr1 kinase, and in doing so provided links between stress response activation and Tyr1 phosphorylation.
[Mh] Termos MeSH primário: Regulação Fúngica da Expressão Gênica
Proteínas Quinases Ativadas por Mitógeno/metabolismo
RNA Polimerase II/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/enzimologia
Estresse Fisiológico
[Mh] Termos MeSH secundário: Quinase 8 Dependente de Ciclina/genética
Quinase 8 Dependente de Ciclina/metabolismo
Genótipo
Complexo Mediador/genética
Complexo Mediador/metabolismo
Proteínas Quinases Ativadas por Mitógeno/genética
Mutação
Fenótipo
Fosforilação
Domínios Proteicos
RNA Polimerase II/química
RNA Polimerase II/genética
RNA Fúngico/genética
RNA Fúngico/metabolismo
Proteínas de Ligação a RNA/genética
Proteínas de Ligação a RNA/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
Proteínas de Saccharomyces cerevisiae/genética
Transdução de Sinais
Fatores de Tempo
Transdução Genética
Tirosina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mediator Complex); 0 (NRD1 protein, S cerevisiae); 0 (RNA, Fungal); 0 (RNA-Binding Proteins); 0 (SSN2 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 42HK56048U (Tyrosine); EC 2.7.11.22 (Cyclin-Dependent Kinase 8); EC 2.7.11.23 (SSN3 protein, S cerevisiae); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); EC 2.7.11.24 (SLT2 protein, S cerevisiae); EC 2.7.7.- (RNA Polymerase II); EC 2.7.7.- (RNA polymerase II largest subunit)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


  9 / 7334 MEDLINE  
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[PMID]:29198561
[Au] Autor:Chou HJ; Donnard E; Gustafsson HT; Garber M; Rando OJ
[Ad] Endereço:Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
[Ti] Título:Transcriptome-wide Analysis of Roles for tRNA Modifications in Translational Regulation.
[So] Source:Mol Cell;68(5):978-992.e4, 2017 Dec 07.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Covalent nucleotide modifications in noncoding RNAs affect a plethora of biological processes, and new functions continue to be discovered even for well-known modifying enzymes. To systematically compare the functions of a large set of noncoding RNA modifications in gene regulation, we carried out ribosome profiling in budding yeast to characterize 57 nonessential genes involved in tRNA modification. Deletion mutants exhibited a range of translational phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibiting the most dramatic translational perturbations. Our data build on prior reports documenting translational upregulation of the nutrient-responsive transcription factor Gcn4 in response to numerous tRNA perturbations, and identify many additional translationally regulated mRNAs throughout the yeast genome. Our data also uncover unexpected roles for tRNA-modifying enzymes in regulation of TY retroelements, and in rRNA 2'-O-methylation. This dataset should provide a rich resource for discovery of additional links between tRNA modifications and gene regulation.
[Mh] Termos MeSH primário: RNA Fúngico/metabolismo
RNA de Transferência/metabolismo
Ribossomos/enzimologia
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/enzimologia
Transcriptoma
tRNA Metiltransferases/metabolismo
[Mh] Termos MeSH secundário: Fatores de Transcrição de Zíper de Leucina Básica/biossíntese
Fatores de Transcrição de Zíper de Leucina Básica/genética
Perfilação da Expressão Gênica/métodos
Regulação Fúngica da Expressão Gênica
Genótipo
Metilação
Mutação
Fenótipo
RNA Fúngico/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Ribossômico/genética
RNA Ribossômico/metabolismo
RNA de Transferência/genética
RNA não Traduzido/genética
RNA não Traduzido/metabolismo
Retroelementos
Ribossomos/genética
Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/biossíntese
Proteínas de Saccharomyces cerevisiae/genética
Sequências Repetidas Terminais
tRNA Metiltransferases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic-Leucine Zipper Transcription Factors); 0 (GCN4 protein, S cerevisiae); 0 (RNA, Fungal); 0 (RNA, Messenger); 0 (RNA, Ribosomal); 0 (RNA, Untranslated); 0 (Retroelements); 0 (Saccharomyces cerevisiae Proteins); 9014-25-9 (RNA, Transfer); EC 2.1.1.- (Trm7 protein, S cerevisiae); EC 2.1.1.- (tRNA Methyltransferases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  10 / 7334 MEDLINE  
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[PMID]:28468764
[Au] Autor:Parker S; Fraczek MG; Wu J; Shamsah S; Manousaki A; Dungrattanalert K; de Almeida RA; Estrada-Rivadeneyra D; Omara W; Delneri D; O'Keefe RT
[Ad] Endereço:Division of Evolution & Genomic Sciences.
[Ti] Título:A resource for functional profiling of noncoding RNA in the yeast .
[So] Source:RNA;23(8):1166-1171, 2017 08.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eukaryotic genomes are extensively transcribed, generating many different RNAs with no known function. We have constructed 1502 molecular barcoded ncRNA gene deletion strains encompassing 443 ncRNAs in the yeast as tools for ncRNA functional analysis. This resource includes deletions of small nuclear RNAs (snRNAs), transfer RNAs (tRNAs), small nucleolar RNAs (snoRNAs), and other annotated ncRNAs as well as the more recently identified stable unannotated transcripts (SUTs) and cryptic unstable transcripts (CUTs) whose functions are largely unknown. Specifically, deletions have been constructed for ncRNAs found in the intergenic regions, not overlapping genes or their promoters (i.e., at least 200 bp minimum distance from the closest gene start codon). The deletion strains carry molecular barcodes designed to be complementary with the protein gene deletion collection enabling parallel analysis experiments. These strains will be useful for the numerous genomic and molecular techniques that utilize deletion strains, including genome-wide phenotypic screens under different growth conditions, pooled chemogenomic screens with drugs or chemicals, synthetic genetic array analysis to uncover novel genetic interactions, and synthetic dosage lethality screens to analyze gene dosage. Overall, we created a valuable resource for the RNA community and for future ncRNA research.
[Mh] Termos MeSH primário: Genoma Fúngico
RNA Fúngico/metabolismo
RNA não Traduzido/metabolismo
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: RNA Fúngico/genética
RNA não Traduzido/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/metabolismo
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Fungal); 0 (RNA, Untranslated)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171223
[Lr] Data última revisão:
171223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1261/rna.061564.117



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