Base de dados : MEDLINE
Pesquisa : D13.444.735.520 [Categoria DeCS]
Referências encontradas : 1035 [refinar]
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  1 / 1035 MEDLINE  
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[PMID]:29225048
[Au] Autor:Gao JF; Gao Y; Qiu JH; Chang QC; Zhang Y; Fang M; Wang CR
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China; Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province, PR China.
[Ti] Título:De novo assembly and functional annotations of the transcriptome of Metorchis orientalis (trematoda: Opisthorchiidae).
[So] Source:Exp Parasitol;184:90-96, 2018 Jan.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Metorchis orientalis is a neglected zoonotic parasite, living in the gallbladder and bile duct of poultry and some mammals as well as humans. In spite of its economic and medical importance, the information known about the transcriptome and genome of M. orientalis is limited. In this study, we performed de novo sequencing, transcriptome assembly and functional annotations of the adult M. orientalis, obtained about 77.4 million high-quality clean reads, among which the length of the transcript contigs ranged from 100 to 11,249 nt with mean length of 373 nt and N50 length of 919 nt. We then assembled 31,943 unigenes, of which 20,009 (62.6%) were annotated by BLASTn and BLASTx searches against the available database. Among these unigenes, 19,795 (62.0%), 3407 (10.7%), 10,620 (33.2%) of them had significant similarity in the NR, NT and Swiss-Prot databases, respectively; 5744 (18.0%) and 4678 (14.6%) unigenes were assigned to GO and COG, respectively; and 9099 (28.5%) unigenes were identified and mapped onto 256 pathways in the KEGG Pathway database. Furthermore, we found that 98 (1.08%) unigenes were related to bile secretion and 5 (0.05%) to primary bile acid biosynthesis pathways category. The characterization of these transcriptomic data has implications for the better understanding of the biology of M. orientalis, and will facilitate the development of intervention agents for this and other pathogenic flukes of human and animal health significance.
[Mh] Termos MeSH primário: Doenças Negligenciadas/parasitologia
Opisthorchidae/fisiologia
Transcriptoma
Infecções por Trematódeos/parasitologia
Zoonoses/parasitologia
[Mh] Termos MeSH secundário: Animais
Ductos Biliares/parasitologia
Biologia Computacional
DNA Complementar/biossíntese
Patos/parasitologia
Doenças dos Peixes/parasitologia
Doenças dos Peixes/transmissão
Peixes
Vesícula Biliar/parasitologia
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Opisthorchidae/genética
Doenças das Aves Domésticas/parasitologia
RNA de Helmintos/genética
RNA de Helmintos/isolamento & purificação
RNA Mensageiro/genética
RNA Mensageiro/isolamento & purificação
Sequenciamento Completo do Exoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Helminth); 0 (RNA, Messenger)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


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[PMID]:29050943
[Au] Autor:Ma YC; Zhang L; Dai LL; Khan RU; Zou CG
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan 650091, China.
[Ti] Título:mir-67 regulates P. aeruginosa avoidance behavior in C. elegans.
[So] Source:Biochem Biophys Res Commun;494(1-2):120-125, 2017 Dec 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogen avoidance behaviors are found throughout the animal kingdom and are important for animal's survival in nature. As a free-living nematode, C. elegans is exposed to a variety of microorganisms, including toxic or pathogenic bacteria, in soil. C. elegans can develop efficient avoidance responses to pathogenic bacteria to minimize the infection risk. However, the role of microRNAs (miRNAs) in pathogen avoidance in C. elegans remains unclear. In this report, we showed that the miRNA mir-67 was involved in a behavioral avoidance response to P. aeruginosa PA14. Exposure to P. aeruginosa PA14 induced the expression of mir-67 in worms. mir-67(n4899) mutants exhibited a reduced ability to avoid P. aeruginosa PA14. By combining quantitative proteomic analysis with miRNA target prediction algorithms, we identified SAX-7/L1CAM, which is transmembrane cell adhesion receptor molecule, as the target of mir-67. Silencing of sax-7 by RNAi on mir-67 mutants rescued avoidance behavioral. Our data demonstrate that the mir-67-SAX-7 pathway modulate the behavioral avoidance response to pathogens, thus providing a new perspective in the role of miRNAs in host-microbe interactions.
[Mh] Termos MeSH primário: Caenorhabditis elegans/genética
Caenorhabditis elegans/fisiologia
MicroRNAs/genética
RNA de Helmintos/genética
[Mh] Termos MeSH secundário: Animais
Aprendizagem da Esquiva/fisiologia
Proteínas de Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/fisiologia
Interações Hospedeiro-Patógeno/genética
Interações Hospedeiro-Patógeno/fisiologia
Moléculas de Adesão de Célula Nervosa/genética
Moléculas de Adesão de Célula Nervosa/fisiologia
Pseudomonas aeruginosa/patogenicidade
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (MicroRNAs); 0 (Neural Cell Adhesion Molecules); 0 (RNA, Helminth); 0 (SAX-7 protein, C elegans)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


  3 / 1035 MEDLINE  
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[PMID]:28733132
[Au] Autor:Guivier E; Lippens C; Faivre B; Sorci G
[Ad] Endereço:Biogéosciences, CNRS UMR 6282, Université de Bourgogne Franche-Comté, 6 Bd Gabriel, 21000 Dijon, France; Physiopathologie des dyslipidémies, INSERM UMR 866, Université de Bourgogne Franche-Comté, 21000 Dijon, France. Electronic address: em.guivier@gmail.com.
[Ti] Título:Plastic and micro-evolutionary responses of a nematode to the host immune environment.
[So] Source:Exp Parasitol;181:14-22, 2017 Oct.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parasitic organisms have to cope with the defences deployed by their hosts and this can be achieved adopting immune evasion strategies or optimal life history traits according to the prevailing pattern of immune-mediated mortality. Parasites often encounter variable immune environments both within and between hosts, promoting the evolution of plastic strategies instead of fixed responses. Here, we explored the plasticity and micro-evolutionary responses of immunomodulatory mechanisms and life history traits to the immune environment provided by the host, using the parasitic nematode Heligmosomoides polygyrus. To test if the parasite responds plastically to the immune environment, we stimulated the systemic inflammatory response of mice and we assessed i) the expression of two genes with candidate immunomodulatory functions (Hp-Tgh2 and Hp-CPI); ii) changes in the number of eggs shed in the faeces. To test if the immune environment induces a micro-evolutionary response in the parasite, we maintained the nematode in mice whose inflammatory response was up- or down-regulated during four generations. We found that H. polygyrus plastically responded to a sudden rise of pro-inflammatory cytokines, up-regulating the expression of two candidate genes involved in the process of immune modulation, and enhancing egg output. At the micro-evolutionary level, parasites maintained in hosts experiencing different levels of inflammation did not have differential expression of Hp-Tgh2 and Hp-CPI genes when infecting unmanipulated, control, mice. However, parasites maintained in mice with an up-regulated inflammation shed more eggs compared to the control line. Overall, our study shows that H. polygyrus can plastically adjust the expression of immunomodulatory genes and life history traits, and responds to selection exerted by the host immune system.
[Mh] Termos MeSH primário: Nematospiroides dubius/imunologia
Infecções por Strongylida/imunologia
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Citocinas/sangue
DNA Complementar/química
DNA Complementar/isolamento & purificação
DNA de Helmintos/química
DNA de Helmintos/isolamento & purificação
Fezes/parasitologia
Feminino
Expressão Gênica
Imunomodulação/genética
Modelos Lineares
Camundongos
Camundongos Endogâmicos BALB C
Nematospiroides dubius/genética
Contagem de Ovos de Parasitas
RNA de Helmintos/isolamento & purificação
RNA Mensageiro/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Inoculações Seriadas
Infecções por Strongylida/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (DNA, Complementary); 0 (DNA, Helminth); 0 (RNA, Helminth); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170723
[St] Status:MEDLINE


  4 / 1035 MEDLINE  
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[PMID]:28645154
[Au] Autor:Brown KC; Svendsen JM; Tucci RM; Montgomery BE; Montgomery TA
[Ad] Endereço:Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
[Ti] Título:ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline.
[So] Source:Nucleic Acids Res;45(15):9093-9107, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to primarily interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We analyzed by high-throughput sequencing the small RNAs associated with ALG-5, ALG-1 and ALG-2, as well as changes in mRNA expression in alg-5, alg-1 and alg-2 mutants. We show that ALG-5 defines a distinct branch of the miRNA pathway affecting the expression of genes involved in immunity, defense, and development. In contrast to ALG-1 and ALG-2, which associate with most miRNAs and have general roles throughout development, ALG-5 interacts with only a small subset of miRNAs and is specifically expressed in the germline where it localizes alongside the piRNA and siRNA machinery at P granules. alg-5 is required for optimal fertility and mutations in alg-5 lead to a precocious transition from spermatogenesis to oogenesis. Our results provide a near-comprehensive analysis of miRNA-Argonaute interactions in C. elegans and reveal a new role for miRNAs in the germline.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Proteínas de Caenorhabditis elegans/genética
Caenorhabditis elegans/genética
Regulação da Expressão Gênica no Desenvolvimento
Células Germinativas/metabolismo
RNA de Helmintos/genética
Proteínas de Ligação a RNA/genética
[Mh] Termos MeSH secundário: Animais
Proteínas Argonauta/metabolismo
Caenorhabditis elegans/classificação
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/metabolismo
Proteínas de Caenorhabditis elegans/metabolismo
Células Germinativas/crescimento & desenvolvimento
Organismos Hermafroditas/genética
Organismos Hermafroditas/crescimento & desenvolvimento
Organismos Hermafroditas/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
MicroRNAs/genética
MicroRNAs/metabolismo
Mutação
Oogênese/genética
Filogenia
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
RNA de Helmintos/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Proteínas de Ligação a RNA/metabolismo
Espermatogênese/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ALG-1 protein, C elegans); 0 (ALG-2 protein, C elegans); 0 (ALG-5 protein, C elegans); 0 (Argonaute Proteins); 0 (Caenorhabditis elegans Proteins); 0 (MicroRNAs); 0 (Protein Isoforms); 0 (RNA, Helminth); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx536


  5 / 1035 MEDLINE  
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Coelho, Paulo Marcos Zech
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[PMID]:28622369
[Au] Autor:Jeremias WJ; Araújo FMG; Queiroz FR; Pais FSM; Mattos ACA; Salim ACM; Coelho PMZ; Oliveira GC; Kusel JR; Guerra-Sá R; Coimbra RS; Babá ÉH
[Ad] Endereço:René Rachou, Oswaldo Cruz Foundation - FIOCRUZ-MG, Belo Horizonte, Minas Gerais, Brazil.
[Ti] Título:Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum.
[So] Source:PLoS One;12(6):e0178829, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the host. Thus, it is very important for the parasite to remain within the skin after entering the host for a period of about 3 days, at which time it can then reach the venous system, migrate to the lungs and, by the end of eighth day post-infection, it reach the portal venous system, while undergoing minimal changes in morphology. However, after just a few days in the portal blood system, the parasite experiences an extraordinary increase in biomass and significant morphological alterations. Therefore, determining the constituents of the portal venous system that may trigger these changes that causes the parasite to consolidate its development inside the vertebrate host, thus causing the disease schistosomiasis, is essential. The present work simulated the conditions found in the portal venous system of the vertebrate host by exposing schistosomula of S. mansoni to in vitro culture in the presence of portal serum of the hamster, Mesocricetus auratus. Two different incubation periods were evaluated, one of 3 hours and one of 12 hours. These time periods were used to mimic the early contact of the parasite with portal serum during the course of natural infection. As a control, parasites were incubated in presence of hamster peripheral serum, in order to compare gene expression signatures between the two conditions. The mRNA obtained from parasites cultured under both conditions were submitted to a whole transcriptome library preparation and sequenced with a next generation platform. On average, nearly 15 million reads were produced per sample and, for the purpose of gene expression quantification, only reads mapped to one location of the transcriptome were considered. After statistical analysis, we found 103 genes differentially expressed by schistosomula cultured for 3 hours and 12 hours in the presence of hamster portal serum. After the subtraction of a second list of genes, also differentially expressed between schistosomula cultured for 3 hours and 12 hours in presence of peripheral serum, a set of 58 genes was finally established. This pattern was further validated for a subset of 17 genes, by measuring gene expression through quantitative real time polymerase chain reaction (qPCR). Processes that were activated by the portal serum stimulus include response to stress, membrane transport, protein synthesis and folding/degradation, signaling, cytoskeleton arrangement, cell adhesion and nucleotide synthesis. Additionally, a smaller number of genes down-regulated under the same condition act on cholinergic signaling, inorganic cation and organic anion membrane transport, cell adhesion and cytoskeleton arrangement. Considering the role of these genes in triggering processes that allow the parasite to quickly adapt, escape the immune response of the host and start maturation into an adult worm after contact with the portal serum, this work may point to unexplored molecular targets for drug discovery and vaccine development against schistosomiasis.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica/efeitos dos fármacos
RNA de Helmintos
RNA Mensageiro
Schistosoma mansoni
Análise de Sequência de RNA/métodos
Soro/química
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Cricetinae
Mesocricetus
RNA de Helmintos/biossíntese
RNA de Helmintos/genética
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
Schistosoma mansoni/genética
Schistosoma mansoni/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Helminth); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178829


  6 / 1035 MEDLINE  
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[PMID]:28622353
[Au] Autor:Ekino T; Yoshiga T; Takeuchi-Kaneko Y; Kanzaki N
[Ad] Endereço:Laboratory of Nematology, Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Saga, Japan.
[Ti] Título:Transmission electron microscopic observation of body cuticle structures of phoretic and parasitic stages of Parasitaphelenchinae nematodes.
[So] Source:PLoS One;12(6):e0179465, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Using transmission electron microscopy, we examined the body cuticle ultrastructures of phoretic and parasitic stages of the parasitaphelenchid nematodes Bursaphelenchus xylophilus, B. conicaudatus, B. luxuriosae, B. rainulfi; an unidentified Bursaphelenchus species, and an unidentified Parasitaphelenchus species. Nematode body cuticles usually consist of three zones, a cortical zone, a median zone, and a basal zone. The phoretic stages of Bursaphelenchus spp., isolated from the tracheal systems of longhorn beetles or the elytra of bark beetles, have a thick and radially striated basal zone. In contrast, the parasitic stage of Parasitaphelenchus sp., isolated from bark beetle hemocoel, has no radial striations in the basal zone. This difference probably reflects the peculiar ecological characteristics of the phoretic stage. A well-developed basal radially striated zone, composed of very closely linked proteins, is the zone closest to the body wall muscle. Therefore, the striation is necessary for the phoretic species to be able to seek, enter, and depart from host/carrier insects, but is not essential for internal parasites in parasitaphelenchid nematodes. Phylogenetic relationships inferred from near-full-length small subunit ribosomal RNA sequences suggest that the cuticle structures of parasitic species have apomorphic characters, e.g., lack of striation in the basal zone, concurrent with the evolution of insect parasitism from a phoretic life history.
[Mh] Termos MeSH primário: Estruturas Animais
Nematoides
Filogenia
RNA de Helmintos
RNA Ribossômico
[Mh] Termos MeSH secundário: Estruturas Animais/metabolismo
Estruturas Animais/ultraestrutura
Animais
Coleópteros/parasitologia
Microscopia Eletrônica de Transmissão
Nematoides/genética
Nematoides/metabolismo
Nematoides/ultraestrutura
RNA de Helmintos/genética
RNA de Helmintos/metabolismo
RNA Ribossômico/genética
RNA Ribossômico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Helminth); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179465


  7 / 1035 MEDLINE  
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[PMID]:28605968
[Au] Autor:Cech G; Molnár K; Székely C
[Ad] Endereço:Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences - Fish Pathology and Parasitology , Hungária krt. 21, H-1143 Budapest , Hungary.
[Ti] Título:Molecular biological studies of adult and metacercarial stages of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae).
[So] Source:Acta Vet Hung;65(2):198-207, 2017 06.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Molnár et al. (2015) reported two types of echinostomatid metacercariae in the lateral line organ of Hungarian fish species. Type 1 metacercariae possessed 27 collar spines and 16 uniform and three larger dorsal spines, whereas Type 2 metacercariae bore 27 collar spines and 19 equal-sized dorsal spines. In the recent work, molecular studies carried out on the ITS region and partial 28S rDNA sequences of two types of echinostomatid metacercariae and the sequences of adult stages of the species of Petasiger Dietz, 1909 collected from cormorants (Phalacrocorax carbo L.) showed that some of the Type 2 metacercariae corresponded to Petasiger exaeretus Dietz, 1909, whereas other morphologically similar metacercariae were identified as Petasiger phalacrocoracis (Yamaguti, 1939). The sequences of the Type 1 metacercariae with three larger dorsal spines could not be identified with any of the known sequences from echinostomatid trematodes.
[Mh] Termos MeSH primário: Echinostomatidae/genética
Metacercárias/genética
[Mh] Termos MeSH secundário: Animais
DNA de Helmintos/genética
DNA Espaçador Ribossômico/genética
Echinostomatidae/fisiologia
Metacercárias/fisiologia
Filogenia
RNA de Helmintos/genética
RNA Ribossômico 28S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer); 0 (RNA, Helminth); 0 (RNA, Ribosomal, 28S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.020


  8 / 1035 MEDLINE  
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[PMID]:28582530
[Au] Autor:Philippe L; Pandarakalam GC; Fasimoye R; Harrison N; Connolly B; Pettitt J; Müller B
[Ad] Endereço:School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.
[Ti] Título:An in vivo genetic screen for genes involved in spliced leader trans-splicing indicates a crucial role for continuous de novo spliced leader RNP assembly.
[So] Source:Nucleic Acids Res;45(14):8474-8483, 2017 Aug 21.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the precise details of SL trans-splicing have yet to be elucidated. In part, this is because the systematic identification of the molecules involved has not previously been possible due to the lack of a specific phenotype associated with defects in this process. We present here a novel GFP-based reporter assay that can monitor SL1 trans-splicing in living C. elegans. Using this assay, we have identified mutants in sna-1 that are defective in SL trans-splicing, and demonstrate that reducing function of SNA-1, SNA-2 and SUT-1, proteins that associate with SL1 RNA and related SmY RNAs, impairs SL trans-splicing. We further demonstrate that the Sm proteins and pICln, SMN and Gemin5, which are involved in small nuclear ribonucleoprotein assembly, have an important role in SL trans-splicing. Taken together these results provide the first in vivo evidence for proteins involved in SL trans-splicing, and indicate that continuous replacement of SL ribonucleoproteins consumed during trans-splicing reactions is essential for effective trans-splicing.
[Mh] Termos MeSH primário: Proteínas de Helminto/genética
RNA de Helmintos/genética
RNA Líder para Processamento/genética
Ribonucleoproteínas/genética
Trans-Splicing
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Sequência de Bases
Caenorhabditis elegans/genética
Caenorhabditis elegans/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Proteínas de Helminto/metabolismo
Microscopia de Fluorescência
Interferência de RNA
Precursores de RNA/genética
Precursores de RNA/metabolismo
RNA de Helmintos/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Líder para Processamento/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ribonucleoproteínas/metabolismo
Ribonucleoproteínas Nucleares Pequenas/genética
Ribonucleoproteínas Nucleares Pequenas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Helminth Proteins); 0 (RNA Precursors); 0 (RNA, Helminth); 0 (RNA, Messenger); 0 (RNA, Spliced Leader); 0 (Ribonucleoproteins); 0 (Ribonucleoproteins, Small Nuclear); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx500


  9 / 1035 MEDLINE  
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[PMID]:28541563
[Au] Autor:Raman P; Zaghab SM; Traver EC; Jose AM
[Ad] Endereço:Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
[Ti] Título:The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans.
[So] Source:Nucleic Acids Res;45(14):8463-8473, 2017 Aug 21.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Long double-stranded RNA (dsRNA) can silence genes of matching sequence upon ingestion in many invertebrates and is therefore being developed as a pesticide. Such feeding RNA interference (RNAi) is best understood in the worm Caenorhabditis elegans, where the dsRNA-binding protein RDE-4 initiates silencing by recruiting an endonuclease to process long dsRNA into short dsRNA. These short dsRNAs are thought to move between cells because muscle-specific rescue of rde-4 using repetitive transgenes enables silencing in other tissues. Here, we extend this observation using additional promoters, report an inhibitory effect of repetitive transgenes, and discover conditions for cell-autonomous silencing in animals with tissue-specific rescue of rde-4. While expression of rde-4(+) in intestine, hypodermis, or neurons using a repetitive transgene can enable silencing also in unrescued tissues, silencing can be inhibited wihin tissues that express a repetitive transgene. Single-copy transgenes that express rde-4(+) in body-wall muscles or hypodermis, however, enable silencing selectively in the rescued tissue but not in other tissues. These results suggest that silencing by the movement of short dsRNA between cells is not an obligatory feature of feeding RNAi in C. elegans. We speculate that similar control of dsRNA movement could modulate tissue-specific silencing by feeding RNAi in other invertebrates.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Interferência de RNA
RNA de Cadeia Dupla/metabolismo
RNA de Helmintos/metabolismo
Proteínas de Ligação a RNA/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Proteínas de Caenorhabditis elegans/genética
Regulação da Expressão Gênica
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Mutação
Regiões Promotoras Genéticas/genética
RNA de Cadeia Dupla/genética
RNA de Helmintos/genética
Proteínas de Ligação a RNA/genética
Transgenes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Membrane Proteins); 0 (RDE-4 protein, C elegans); 0 (RNA, Double-Stranded); 0 (RNA, Helminth); 0 (RNA-Binding Proteins); 0 (SID-1 protein, C elegans)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx484


  10 / 1035 MEDLINE  
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[PMID]:28376858
[Au] Autor:Gu K; Li Y; Driguez P; Zeng Q; Yu X; Sun H; Cai L; He Y; Wang W; McManus DP
[Ad] Endereço:Department of Parasitology, Xiangya School of Medicine, Central South University (CSU), 410013, Tongzipo Road 172#, Changsha, Hunan, People's Republic of China.
[Ti] Título:Clinical diagnostic value of viable Schistosoma japonicum eggs detected in host tissues.
[So] Source:BMC Infect Dis;17(1):244, 2017 Apr 04.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Schistosomiasis, one of the neglected tropical diseases, is endemic in more than 70 countries. However, the clinical diagnosis of patients with a low degree of infection is an unsolved technical problem. In areas endemic for schistosomiasis japonica, proctoscopy detection of eggs has been one method used for clinical diagnosis. However, it is often a challenge to find typical live eggs and it is difficult to distinguish live eggs from large numbers of partially degraded and/or completely degraded eggs within colon biopsy tissue. To address this problem, we tested six different morphological and biochemical/molecular markers (ALP; morphological characteristics of egg; CalS (calcified substance); AOS (antioxidase); SDHG (succinic dehydrogenase) and SjR2 mRNA (retrotransposons 2 of S.japonicum genome mRNA)), including four new markers (CalS; AOS; SDHG and SjR2 mRNA.), to determine the viability of S. japonicum eggs deposited in human and mouse colon tissues. Our ultimate aim is to obtain a new method that is more sensitive, practical and accurate to clinically diagnose schistosomiasis. METHODS: Tissue samples were collected from mice at six different time points during S. japonicum infection with or without treatment with praziquantel (PZQ). Four new biochemical or molecular markers were used for the detection of egg viability from mouse liver and intestinal samples: CalS; AOS; SDHG and SjR2 mRNA. Subsequently, all markers were employed for the detection and analysis of eggs deposited in biopsy materials from patients with suspected schistosomiasis japonica for clinical evaluation. Microscopic examination of the egg morphology, worm burden in vivo and ALP (alkaline phosphatase) levels were used as a reference standard to evaluate the sensitivity and reliability of four new markers detecting egg viability. RESULTS: The results of the study showed that the morphology of S. japonicum eggs deposited in tissues of hosts with schistosomiasis, especially cases with chronic schistosomiasis, is complex and egg viability is difficult to judge morphologically, particularly eggs with a fuzzy structure or partially modified eggs. We found that the majority of the viable schistosome eggs determined by four new markers (CalS, AOS, SDHG and SjR2 mRNA) were morphologically difficult to identify. CONCLUSIONS: Among the markers, the most sensitive and specific method was the detection of SjR2 mRNA and the most simple, rapid and practical method was the detection of SDHG. Therefore, the detection of SDHG is the most practical for clinical application and its use could improve the accuracy in diagnosing active schistosome infection.
[Mh] Termos MeSH primário: Schistosoma japonicum
Esquistossomose Japônica/diagnóstico
[Mh] Termos MeSH secundário: Animais
Biomarcadores/análise
Biópsia
Colo/parasitologia
Feminino
Seres Humanos
Mucosa Intestinal/parasitologia
Fígado/parasitologia
Masculino
Camundongos
Óvulo
Praziquantel/uso terapêutico
RNA de Helmintos/análise
RNA Mensageiro/análise
Reto/parasitologia
Reprodutibilidade dos Testes
Esquistossomose Japônica/tratamento farmacológico
Esquistossomose Japônica/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (RNA, Helminth); 0 (RNA, Messenger); 6490C9U457 (Praziquantel)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2362-4



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