Base de dados : MEDLINE
Pesquisa : D13.444.735.635.575 [Categoria DeCS]
Referências encontradas : 156 [refinar]
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[PMID]:29229384
[Au] Autor:Yang J; Zhang M; Wang X
[Ad] Endereço:School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui 230601, China.
[Ti] Título:Crystal structure of the chloroplast RNA editing factor MORF2.
[So] Source:Biochem Biophys Res Commun;495(2):2038-2043, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA editing is a post-transcription process that alters the genetic information on RNA molecules. In plastids and mitochondria of flowering plants, the multiple organellar RNA editing factors (MORFs) interact with the PLS-type pentatricopeptide repeat (PPR) proteins and participate in RNA editing of cytidine-to-uridine conversion. The PPR proteins recognize cytidine targets around the editing sites, and the MORF proteins modulate the RNA-binding activity of the PPR proteins. Here, we report the structure of the Arabidopsis thaliana chloroplast MORF2 at 2.4 Å resolution. The structure, adopting typical MORF-box fold as observed in mitochondrial MORF1 and chloroplast MORF9, reveals an MORF1-like dimerization mode. The difference between the two dimerization modes can be attributed to F157 (corresponding F162 in MORF1 and W160 in MORF9), which causes a 60° shift upon dimerization. This observation, together with the PPR-MORF2 model, suggests a dimer-to-monomer transition during RNA editosome formation.
[Mh] Termos MeSH primário: Arabidopsis/química
Arabidopsis/ultraestrutura
Cloroplastos/ultraestrutura
RNA de Cloroplastos/ultraestrutura
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis
Cloroplastos/química
Proteínas Mitocondriais
Conformação Proteica
Edição de RNA
RNA de Cloroplastos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (MORF2 protein, Arabidopsis); 0 (Mitochondrial Proteins); 0 (RNA, Chloroplast)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:28985404
[Au] Autor:Cavaiuolo M; Kuras R; Wollman FA; Choquet Y; Vallon O
[Ad] Endereço:Unité Mixte de Recherche 7141, CNRS/UPMC, Institut de Biologie Physico-Chimique, F-75005 Paris, France.
[Ti] Título:Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.
[So] Source:Nucleic Acids Res;45(18):10783-10799, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In Chlamydomonas reinhardtii, regulation of chloroplast gene expression is mainly post-transcriptional. It requires nucleus-encoded trans-acting protein factors for maturation/stabilization (M factors) or translation (T factors) of specific target mRNAs. We used long- and small-RNA sequencing to generate a detailed map of the transcriptome. Clusters of sRNAs marked the 5' end of all mature mRNAs. Their absence in M-factor mutants reflects the protection of transcript 5' end by the cognate factor. Enzymatic removal of 5'-triphosphates allowed identifying those cosRNA that mark a transcription start site. We detected another class of sRNAs derived from low abundance transcripts, antisense to mRNAs. The formation of antisense sRNAs required the presence of the complementary mRNA and was stimulated when translation was inhibited by chloramphenicol or lincomycin. We propose that they derive from degradation of double-stranded RNAs generated by pairing of antisense and sense transcripts, a process normally hindered by the traveling of the ribosomes. In addition, chloramphenicol treatment, by freezing ribosomes on the mRNA, caused the accumulation of 32-34 nt ribosome-protected fragments. Using this 'in vivo ribosome footprinting', we identified the function and molecular target of two candidate trans-acting factors.
[Mh] Termos MeSH primário: Chlamydomonas reinhardtii/genética
RNA de Cloroplastos/metabolismo
Pequeno RNA não Traduzido/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Chlamydomonas reinhardtii/crescimento & desenvolvimento
Chlamydomonas reinhardtii/metabolismo
Proteínas de Cloroplastos/metabolismo
Perfilação da Expressão Gênica
Inibidores da Síntese de Ácido Nucleico/farmacologia
Processos Fototróficos
Proteínas de Plantas/metabolismo
Biossíntese de Proteínas
RNA Antissenso/metabolismo
RNA Mensageiro/metabolismo
RNA Ribossômico/metabolismo
RNA de Transferência/metabolismo
Ribossomos/metabolismo
Análise de Sequência de RNA
Transcrição Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chloroplast Proteins); 0 (Nucleic Acid Synthesis Inhibitors); 0 (Plant Proteins); 0 (RNA, Antisense); 0 (RNA, Chloroplast); 0 (RNA, Messenger); 0 (RNA, Ribosomal); 0 (RNA, Small Untranslated); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx668


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[PMID]:28335814
[Au] Autor:Prazeres M; Ainsworth T; Roberts TE; Pandolfi JM; Leggat W
[Ad] Endereço:College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia. martina.defreitasprazeres@jcu.edu.au.
[Ti] Título:Symbiosis and microbiome flexibility in calcifying benthic foraminifera of the Great Barrier Reef.
[So] Source:Microbiome;5(1):38, 2017 Mar 23.
[Is] ISSN:2049-2618
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Symbiosis is a phenomenon that allows organisms to colonise a wide range of environments and occupy a variety of ecological niches in marine environments. Large benthic foraminifera (LBF) are crucial marine calcifiers that rely on photo-endosymbionts for growth and calcification, yet the influence of environmental conditions in shaping their interactions with prokaryotic and eukaryotic associates is poorly known. RESULTS: Here, we used next-generation sequencing to identify eukaryotic photosynthesizing and prokaryotic microbes associated with the common LBF Amphistegina lobifera across a physio-chemical gradient on the Great Barrier Reef (GBR). We collected samples from three reef sites located in the inner-, mid- and outer-shelf regions of the northern section of the GBR. Results showed the consistent presence of Bacillaryophyta as the main eukaryotic taxa associated with A. lobifera across all reef sites analysed; however, the abundance and the diversity of prokaryotic organisms varied among reef sites. Inner-shelf specimens showed the highest diversity of prokaryote associates, with a total of 231 genotypes in their core microbiome. A total of 30 taxa were identified in the core microbiome across all reef sites. Within these taxa, Proteobacteria was the most abundant bacteria present. The presence of groups such as Actinobacteria was significantly correlated with inner-shelf populations, whereas the abundance of Bacteroidetes and Firmicutes was associated with A. lobifera collected from mid- and outer-shelf reef sites. CONCLUSIONS: We found that benthic foraminifera form stable and persistent symbiosis with eukaryotic partners, but flexible and site-specific associations with prokaryotic microbes that likely influence the ecological success of these crucial calcifying organisms on the GBR.
[Mh] Termos MeSH primário: Actinobacteria/isolamento & purificação
Bacteroidetes/isolamento & purificação
Firmicutes/isolamento & purificação
Foraminíferos/fisiologia
Microbiota/fisiologia
Proteobactérias/isolamento & purificação
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Actinobacteria/genética
Bacteroidetes/classificação
Bacteroidetes/genética
Sequência de Bases
Recifes de Corais
Firmicutes/classificação
Firmicutes/genética
Foraminíferos/genética
Sequenciamento de Nucleotídeos em Larga Escala
Microbiota/genética
Proteobactérias/classificação
Proteobactérias/genética
RNA de Cloroplastos/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Chloroplast); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1186/s40168-017-0257-7


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[PMID]:27899576
[Au] Autor:Cognat V; Morelle G; Megel C; Lalande S; Molinier J; Vincent T; Small I; Duchêne AM; Maréchal-Drouard L
[Ad] Endereço:Institut de biologie moléculaire des plantes, UPR 2357 CNRS, associated with Strasbourg University, 12 rue du Général Zimmer 67084 Strasbourg cedex, France.
[Ti] Título:The nuclear and organellar tRNA-derived RNA fragment population in Arabidopsis thaliana is highly dynamic.
[So] Source:Nucleic Acids Res;45(6):3460-3472, 2017 Apr 07.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the expanding repertoire of small noncoding RNAs (ncRNAs), tRNA-derived RNA fragments (tRFs) have been identified in all domains of life. Their existence in plants has been already proven but no detailed analysis has been performed. Here, short tRFs of 19-26 nucleotides were retrieved from Arabidopsis thaliana small RNA libraries obtained from various tissues, plants submitted to abiotic stress or fractions immunoprecipitated with ARGONAUTE 1 (AGO1). Large differences in the tRF populations of each extract were observed. Depending on the tRNA, either tRF-5D (due to a cleavage in the D region) or tRF-3T (via a cleavage in the T region) were found and hot spots of tRNA cleavages have been identified. Interestingly, up to 25% of the tRFs originate from plastid tRNAs and we provide evidence that mitochondrial tRNAs can also be a source of tRFs. Very specific tRF-5D deriving not only from nucleus-encoded but also from plastid-encoded tRNAs are strongly enriched in AGO1 immunoprecipitates. We demonstrate that the organellar tRFs are not found within chloroplasts or mitochondria but rather accumulate outside the organelles. These observations suggest that some organellar tRFs could play regulatory functions within the plant cell and may be part of a signaling pathway.
[Mh] Termos MeSH primário: Arabidopsis/genética
Núcleo Celular/metabolismo
RNA de Transferência/metabolismo
RNA não Traduzido/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Proteínas Argonauta/metabolismo
Núcleo Celular/genética
Folhas de Planta/genética
Folhas de Planta/metabolismo
Raízes de Plantas/genética
Raízes de Plantas/metabolismo
Plastídeos/metabolismo
RNA/metabolismo
RNA de Cloroplastos/metabolismo
RNA de Transferência/química
RNA não Traduzido/química
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AGO1 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Argonaute Proteins); 0 (RNA, Chloroplast); 0 (RNA, Untranslated); 0 (RNA, mitochondrial); 63231-63-0 (RNA); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkw1122


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[PMID]:28105907
[Au] Autor:Hajieghrari B; Farrokhi N; Goliaei B; Kavousi K
[Ti] Título:Identification and Characterization of Novel miRNAs in Chlamydomonas reinhardtii by Computational Methods.
[So] Source:Microrna;5(1):66-77, 2016.
[Is] ISSN:2211-5374
[Cp] País de publicação:United Arab Emirates
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: MicroRNAs (miRNAs) are endogenous small non-coding RNAs with 18-24 nucleotides in length, which have important roles in posttranscriptional gene regulation. The resemblance of miRNA biogenesis in unicellular green algae and those in plants suggests probable evolutionary conserved pathways. This conservation provides a ground towards prediction of new homologs via computational biology. METHODS: Here, conserved miRNA genes in Chlamydomonas reinhardtii and plants were examined through homology alignment. Previously known and unique plant miRNAs were BLASTed against expressed sequence tags (ESTs) and genomic survey sequences (GSSs) of C. reinhardtii. All candidate sequences with appropriate fold back structures were screened according to a series of miRNA filtering criteria. RESULTS: Homologous miRNAs (17), belonging to 9 miRNA gene families were predicted. Interestingly and for the first time, a miRNA family of genes was localized to chloroplast. Again and for the first time, here we report identification of C. reinhardtii miRNA orthologs in plants and animals. miRNA target genes were identified based on their sequence complementarities to the respective miRNAs using psRNATarget against C. reinhardtii, Unigene, and DFCI Gene Index (CHRGI). Totally, 152 potential target sites were identified. From the predicted miRNAs, 7 miRNAs had no target sequence in C. reinhardtii protein coding genes. CONCLUSION: Identifying miRNA and their target transcript(s) would be useful for other research concerned with the function and regulatory mechanisms of C. reinhardtii miRNAs and helps researchers to better understand the nature of its extensive metabolic flexibility and environmental compatibility to survive in distinct environmental niches and nutrient availability.
[Mh] Termos MeSH primário: Chlamydomonas reinhardtii/genética
Cloroplastos/genética
Biologia Computacional/métodos
MicroRNAs/genética
RNA de Cloroplastos/genética
[Mh] Termos MeSH secundário: Sequência de Bases/genética
Etiquetas de Sequências Expressas
Regulação da Expressão Gênica de Plantas
MicroRNAs/classificação
MicroRNAs/isolamento & purificação
RNA de Cloroplastos/classificação
RNA de Cloroplastos/isolamento & purificação
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Chloroplast)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


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[PMID]:27329857
[Au] Autor:Knie N; Grewe F; Fischer S; Knoop V
[Ad] Endereço:Abteilung Molekulare Evolution, IZMB - Institut für Zelluläre und Molekulare Botanik, Universität Bonn, Kirschallee 1, D-53115, Bonn, Germany.
[Ti] Título:Reverse U-to-C editing exceeds C-to-U RNA editing in some ferns - a monilophyte-wide comparison of chloroplast and mitochondrial RNA editing suggests independent evolution of the two processes in both organelles.
[So] Source:BMC Evol Biol;16(1):134, 2016 Jun 21.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: RNA editing by C-to-U conversions is nearly omnipresent in land plant chloroplasts and mitochondria, where it mainly serves to reconstitute conserved codon identities in the organelle mRNAs. Reverse U-to-C RNA editing in contrast appears to be restricted to hornworts, some lycophytes, and ferns (monilophytes). A well-resolved monilophyte phylogeny has recently emerged and now allows to trace the side-by-side evolution of both types of pyrimidine exchange editing in the two endosymbiotic organelles. RESULTS: Our study of RNA editing in four selected mitochondrial genes show a wide spectrum of divergent RNA editing frequencies including a dominance of U-to-C over the canonical C-to-U editing in some taxa like the order Schizaeales. We find that silent RNA editing leaving encoded amino acids unchanged is highly biased with more than ten-fold amounts of silent C-to-U over U-to-C edits. In full contrast to flowering plants, RNA editing frequencies are low in early-branching monilophyte lineages but increase in later emerging clades. Moreover, while editing rates in the two organelles are usually correlated, we observe uncoupled evolution of editing frequencies in fern mitochondria and chloroplasts. Most mitochondrial RNA editing sites are shared between the recently emerging fern orders whereas chloroplast editing sites are mostly clade-specific. Finally, we observe that chloroplast RNA editing appears to be completely absent in horsetails (Equisetales), the sister clade of all other monilophytes. CONCLUSIONS: C-to-U and U-to-C RNA editing in fern chloroplasts and mitochondria follow disinct evolutionary pathways that are surprisingly different from what has previously been found in flowering plants. The results call for careful differentiation of the two types of RNA editing in the two endosymbiotic organelles in comparative evolutionary studies.
[Mh] Termos MeSH primário: Cloroplastos/genética
Gleiquênias/genética
Mitocôndrias/genética
RNA de Plantas/metabolismo
[Mh] Termos MeSH secundário: Evolução Biológica
Citosina
Equisetum/classificação
Magnoliopsida/genética
Dados de Sequência Molecular
Filogenia
RNA/metabolismo
Edição de RNA
RNA de Cloroplastos/genética
RNA Nuclear Pequeno/metabolismo
Uracila
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Chloroplast); 0 (RNA, Plant); 0 (RNA, Small Nuclear); 0 (RNA, mitochondrial); 56HH86ZVCT (Uracil); 63231-63-0 (RNA); 8J337D1HZY (Cytosine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160623
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-016-0707-z


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[PMID]:27235415
[Au] Autor:Ruwe H; Wang G; Gusewski S; Schmitz-Linneweber C
[Ad] Endereço:Molekulare Genetik, Institut für Biologie, Humboldt-Universität zu Berlin, Philippstr. 11-13, 10115 Berlin, Germany hannes.ruwe@hu-berlin.de.
[Ti] Título:Systematic analysis of plant mitochondrial and chloroplast small RNAs suggests organelle-specific mRNA stabilization mechanisms.
[So] Source:Nucleic Acids Res;44(15):7406-17, 2016 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Land plant organellar genomes encode a small number of genes, many of which are essential for respiration and photosynthesis. Organellar gene expression is characterized by a multitude of RNA processing events that lead to stable, translatable transcripts. RNA binding proteins (RBPs), have been shown to generate and protect transcript termini and eventually induce the accumulation of short RNA footprints. We applied knowledge of such RBP-derived footprints to develop software (sRNA miner) that enables identification of RBP footprints, or other clusters of small RNAs, in organelles. We used this tool to determine mitochondrial and chloroplast cosRNAs (clustered organellar sRNAs) in Arabidopsis. We found that in mitochondria, cosRNAs coincide with transcript 3'-ends, but are largely absent from 5'-ends. In chloroplasts this bias is absent, suggesting a different mode of 5' processing, possibly owing to different sets of RNases. Furthermore, we identified a large number of cosRNAs that represent silenced insertions of mitochondrial DNA in the nuclear genome of Arabidopsis. Steady-state RNA analyses demonstrate that cosRNAs display differential accumulation during development. Finally, we demonstrate that the chloroplast RBP PPR10 associates in vivo with its cognate cosRNA. A hypothetical role of cosRNAs as competitors of mRNAs for PPR proteins is discussed.
[Mh] Termos MeSH primário: Arabidopsis/citologia
Arabidopsis/genética
Cloroplastos/genética
Mitocôndrias/genética
Estabilidade de RNA
RNA de Cloroplastos/metabolismo
RNA/metabolismo
Software
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/metabolismo
Cloroplastos/metabolismo
DNA Mitocondrial/genética
DNA Mitocondrial/metabolismo
Conjuntos de Dados como Assunto
Genoma Mitocondrial/genética
Genoma de Planta/genética
Mitocôndrias/metabolismo
Proteínas de Ligação a RNA/metabolismo
Ribonucleases/metabolismo
Transcrição Genética/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (DNA, Mitochondrial); 0 (PPR10 protein, Arabidopsis); 0 (RNA, Chloroplast); 0 (RNA, mitochondrial); 0 (RNA-Binding Proteins); 63231-63-0 (RNA); EC 3.1.- (Ribonucleases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160529
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkw466


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[PMID]:26809609
[Au] Autor:Hein A; Polsakiewicz M; Knoop V
[Ad] Endereço:IZMB - Institut für Zelluläre und Molekulare Botanik, Abteilung Molekulare Evolution, Universität Bonn, Kirschallee 1, D-53115, Bonn, Germany. ahein@uni-bonn.de.
[Ti] Título:Frequent chloroplast RNA editing in early-branching flowering plants: pilot studies on angiosperm-wide coexistence of editing sites and their nuclear specificity factors.
[So] Source:BMC Evol Biol;16:23, 2016 Jan 25.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: RNA editing by cytidine-to-uridine conversions is an essential step of RNA maturation in plant organelles. Some 30-50 sites of C-to-U RNA editing exist in chloroplasts of flowering plant models like Arabidopsis, rice or tobacco. We now predicted significantly more RNA editing in chloroplasts of early-branching angiosperm genera like Amborella, Calycanthus, Ceratophyllum, Chloranthus, Illicium, Liriodendron, Magnolia, Nuphar and Zingiber. Nuclear-encoded RNA-binding pentatricopeptide repeat (PPR) proteins are key editing factors expected to coevolve with their cognate RNA editing sites in the organelles. RESULTS: With an extensive chloroplast transcriptome study we identified 138 sites of RNA editing in Amborella trichopoda, approximately the 3- to 4-fold of cp editing in Arabidopsis thaliana or Oryza sativa. Selected cDNA studies in the other early-branching flowering plant taxa furthermore reveal a high diversity of early angiosperm RNA editomes. Many of the now identified editing sites in Amborella have orthologues in ferns, lycophytes or hornworts. We investigated the evolution of CRR28 and RARE1, two known Arabidopsis RNA editing factors responsible for cp editing events ndhBeU467PL, ndhDeU878SL and accDeU794SL, respectively, all of which we now found conserved in Amborella. In a phylogenetically wide sampling of 65 angiosperm genomes we find evidence for only one single loss of CRR28 in chickpea but several independent losses of RARE1, perfectly congruent with the presence of their cognate editing sites in the respective cpDNAs. CONCLUSION: Chloroplast RNA editing is much more abundant in early-branching than in widely investigated model flowering plants. RNA editing specificity factors can be traced back for more than 120 million years of angiosperm evolution and show highly divergent patterns of evolutionary losses, matching the presence of their target editing events.
[Mh] Termos MeSH primário: Magnoliopsida/genética
Edição de RNA
RNA de Cloroplastos/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Evolução Biológica
Núcleo Celular/metabolismo
Cloroplastos/genética
DNA de Cloroplastos/genética
Magnoliopsida/citologia
Magnoliopsida/fisiologia
Dados de Sequência Molecular
Nucleoproteínas/metabolismo
Filogenia
Projetos Piloto
RNA de Cloroplastos/química
Proteínas de Ligação a RNA/genética
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (Nucleoproteins); 0 (RNA, Chloroplast); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-016-0589-0


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[PMID]:26643268
[Au] Autor:Zoschke R; Watkins KP; Miranda RG; Barkan A
[Ad] Endereço:Institute of Molecular Biology, University of Oregon, Eugene, OR, 97403, USA.
[Ti] Título:The PPR-SMR protein PPR53 enhances the stability and translation of specific chloroplast RNAs in maize.
[So] Source:Plant J;85(5):594-606, 2016 Mar.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pentatricopeptide repeat (PPR) proteins are helical repeat proteins that bind RNA and influence gene expression in mitochondria and chloroplasts. Several PPR proteins in plants harbor a carboxy-terminal small-MutS-related (SMR) domain, but the functions of the SMR appendage are unknown. To address this issue, we studied a maize PPR-SMR protein denoted PPR53 (GRMZM2G438524), which is orthologous to the Arabidopsis protein SOT1 (AT5G46580). Null ppr53 alleles condition a chlorotic, seedling-lethal phenotype and a reduction in plastid ribosome content. Plastome-wide transcriptome and translatome analyses revealed strong defects in the expression of the ndhA and rrn23 genes, which were superimposed on secondary effects resulting from a decrease in plastid ribosome content. Transcripts with processed 5'-ends mapping approximately 70 nucleotides upstream of rrn23 and ndhA are absent in ppr53 mutants, and the translational efficiency of the residual ndhA mRNAs is reduced. Recombinant PPR53 binds with high affinity and specificity to the 5' proximal region of the PPR53-dependent 23S rRNA, suggesting that PPR53 protects this RNA via a barrier mechanism similar to that described for several PPR proteins lacking SMR motifs. However, recombinant PPR53 did not bind with high affinity to the ndhA 5' untranslated region, suggesting that PPR53's RNA-stabilization and translation-enhancing effects at the ndhA locus involve the participation of other factors.
[Mh] Termos MeSH primário: Proteínas de Plantas/genética
Biossíntese de Proteínas/genética
RNA de Cloroplastos/genética
Zea mays/genética
[Mh] Termos MeSH secundário: Motivos de Aminoácidos/genética
Sequência de Aminoácidos
Regulação da Expressão Gênica de Plantas
Genoma de Cloroplastos/genética
Immunoblotting
Mutação
Proteínas de Plantas/metabolismo
Ligação Proteica
Estabilidade de RNA/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência de Aminoácidos
Transcriptoma/genética
Zea mays/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Chloroplast)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151209
[St] Status:MEDLINE
[do] DOI:10.1111/tpj.13093


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[PMID]:26147377
[Au] Autor:Carlotto N; Wirth S; Furman N; Ferreyra Solari N; Ariel F; Crespi M; Kobayashi K
[Ad] Endereço:Laboratorio de Agrobiotecnología, Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA-CONICET-UBA), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
[Ti] Título:The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing.
[So] Source:Plant Cell Environ;39(1):165-73, 2016 Jan.
[Is] ISSN:1365-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/enzimologia
Regulação da Expressão Gênica de Plantas
Plasmodesmos/metabolismo
RNA Helicases/genética
Processamento de RNA
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Transporte Biológico
Cloroplastos/enzimologia
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Genes Reporter
Íntrons/genética
Mutação
Fotossíntese
Plantas Geneticamente Modificadas
RNA Helicases/metabolismo
RNA de Cloroplastos/genética
RNA de Cloroplastos/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/enzimologia
Plântulas/genética
Plântulas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (RNA, Chloroplast); EC 2.7.7.- (INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis); EC 3.6.4.13 (DEAD-box RNA Helicases); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150707
[St] Status:MEDLINE
[do] DOI:10.1111/pce.12603



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