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  1 / 40716 MEDLINE  
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[PMID]:29484747
[Au] Autor:Hirad AH; Ahmad J; Alkhedhairy AA; Bahkali AH; Khan ST
[Ad] Endereço:Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:Bacterial isolates exhibiting multidrug resistance, hemolytic activity, and high 16S rRNA gene similarity with well-known pathogens found in camel milk samples of Riyadh region.
[So] Source:APMIS;126(3):215-226, 2018 Mar.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 10  CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit ß-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Leite/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillus anthracis/genética
Bacillus anthracis/isolamento & purificação
Bactérias/genética
Carga Bacteriana
Brucella/genética
Brucella/isolamento & purificação
Camelus
Citrobacter freundii/genética
Citrobacter freundii/isolamento & purificação
Microbiologia de Alimentos
Seres Humanos
Listeria monocytogenes/genética
Listeria monocytogenes/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Micrococcaceae/genética
Micrococcaceae/isolamento & purificação
Pasteurização
RNA Ribossômico 16S/genética
Arábia Saudita
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180228
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12802


  2 / 40716 MEDLINE  
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[PMID]:29381713
[Au] Autor:Nishida A; Thiel V; Nakagawa M; Ayukawa S; Yamamura M
[Ad] Endereço:Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology, Kanagawa, Japan.
[Ti] Título:Effect of light wavelength on hot spring microbial mat biodiversity.
[So] Source:PLoS One;13(1):e0191650, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hot spring associated phototrophic microbial mats are purely microbial communities, in which phototrophic bacteria function as primary producers and thus shape the community. The microbial mats at Nakabusa hot springs in Japan harbor diverse photosynthetic bacteria, mainly Thermosynechococcus, Chloroflexus, and Roseiflexus, which use light of different wavelength for energy conversion. The aim of this study was to investigate the effect of the phototrophs on biodiversity and community composition in hot spring microbial mats. For this, we specifically activated the different phototrophs by irradiating the mats with different wavelengths in situ. We used 625, 730, and 890 nm wavelength LEDs alone or in combination and confirmed the hypothesized increase in relative abundance of different phototrophs by 16S rRNA gene sequencing. In addition to the increase of the targeted phototrophs, we studied the effect of the different treatments on chemotrophic members. The specific activation of Thermosynechococcus led to increased abundance of several other bacteria, whereas wavelengths specific to Chloroflexus and Roseiflexus induced a decrease in >50% of the community members as compared to the dark conditions. This suggests that the growth of Thermosynechococcus at the surface layer benefits many community members, whereas less benefit is obtained from an increase in filamentous anoxygenic phototrophs Chloroflexus and Roseiflexus. The increases in relative abundance of chemotrophs under different light conditions suggest a relationship between the two groups. Aerobic chemoheterotrophs such as Thermus sp. and Meiothermus sp. are thought to benefit from aerobic conditions and organic carbon in the form of photosynthates by Thermosynechococcus, while the oxidation of sulfide and production of elemental sulfur by filamentous anoxygenic phototrophs benefit the sulfur-disproportionating Caldimicrobium thiodismutans. In this study, we used an experimental approach under controlled environmental conditions for the analysis of natural microbial communities, which proved to be a powerful tool to study interspecies relationships in the microbiome.
[Mh] Termos MeSH primário: Biodiversidade
Fontes Termais/microbiologia
Luz
[Mh] Termos MeSH secundário: Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191650


  3 / 40716 MEDLINE  
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[PMID]:29377905
[Au] Autor:Bird C; Darling KF; Russell AD; Fehrenbacher JS; Davis CV; Free A; Ngwenya BT
[Ad] Endereço:School of Geosciences, University of Edinburgh, Edinburgh, United Kingdom.
[Ti] Título:16S rRNA gene metabarcoding and TEM reveals different ecological strategies within the genus Neogloboquadrina (planktonic foraminifer).
[So] Source:PLoS One;13(1):e0191653, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Uncovering the complexities of trophic and metabolic interactions among microorganisms is essential for the understanding of marine biogeochemical cycling and modelling climate-driven ecosystem shifts. High-throughput DNA sequencing methods provide valuable tools for examining these complex interactions, although this remains challenging, as many microorganisms are difficult to isolate, identify and culture. We use two species of planktonic foraminifera from the climatically susceptible, palaeoceanographically important genus Neogloboquadrina, as ideal test microorganisms for the application of 16S rRNA gene metabarcoding. Neogloboquadrina dutertrei and Neogloboquadrina incompta were collected from the California Current and subjected to either 16S rRNA gene metabarcoding, fluorescence microscopy, or transmission electron microscopy (TEM) to investigate their species-specific trophic interactions and potential symbiotic associations. 53-99% of 16S rRNA gene sequences recovered from two specimens of N. dutertrei were assigned to a single operational taxonomic unit (OTU) from a chloroplast of the phylum Stramenopile. TEM observations confirmed the presence of numerous intact coccoid algae within the host cell, consistent with algal symbionts. Based on sequence data and observed ultrastructure, we taxonomically assign the putative algal symbionts to Pelagophyceae and not Chrysophyceae, as previously reported in this species. In addition, our data shows that N. dutertrei feeds on protists within particulate organic matter (POM), but not on bacteria as a major food source. In total contrast, of OTUs recovered from three N. incompta specimens, 83-95% were assigned to bacterial classes Alteromonadales and Vibrionales of the order Gammaproteobacteria. TEM demonstrates that these bacteria are a food source, not putative symbionts. Contrary to the current view that non-spinose foraminifera are predominantly herbivorous, neither N. dutertrei nor N. incompta contained significant numbers of phytoplankton OTUs. We present an alternative view of their trophic interactions and discuss these results within the context of modelling global planktonic foraminiferal abundances in response to high-latitude climate change.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico
RNA Ribossômico 16S/genética
Rhizaria/genética
[Mh] Termos MeSH secundário: Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191653


  4 / 40716 MEDLINE  
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[PMID]:28841530
[Au] Autor:Yang X; Wei H; Zhu C; Geng B
[Ad] Endereço:Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
[Ti] Título:Biodegradation of atrazine by the novel Citricoccus sp. strain TT3.
[So] Source:Ecotoxicol Environ Saf;147:144-150, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A previously undescribed atrazine-degrading bacterial strain TT3 capable of growing with atrazine as its sole nitrogen source was isolated from soil at the wastewater outfall of a pesticide factory in China. Phenotypic characterization and 16S rRNA gene sequencing indicated that the isolate belonged to the genus Citricoccus. Polymerase chain reaction (PCR) analysis revealed that TT3 contained the atrazine-degrading genes trzN, atzB, and atzC. The range for growth and atrazine degradation of TT3 was found to be pH 6.0-11.0, with a preference for alkaline conditions. At 30°C and pH 7.0, the strain removed 50mg/L atrazine in 66h with 1% inoculum. These results demonstrate that Citricoccus sp. TT3 has great potential for bioremediation of atrazine-contaminated sites, particularly in alkaline environments. To the best of our knowledge, there are no previous reports of Citricoccus strains that degrade atrazine, and therefore this work provides a novel candidate for atrazine bioremediation.
[Mh] Termos MeSH primário: Atrazina/análise
Herbicidas/análise
Micrococcaceae/crescimento & desenvolvimento
Microbiologia do Solo
Poluentes do Solo/análise
[Mh] Termos MeSH secundário: Atrazina/metabolismo
Biodegradação Ambiental
China
Herbicidas/metabolismo
Micrococcaceae/isolamento & purificação
RNA Ribossômico 16S/genética
Solo/química
Poluentes do Solo/metabolismo
Eliminação de Resíduos Líquidos
Águas Residuais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (RNA, Ribosomal, 16S); 0 (Soil); 0 (Soil Pollutants); 0 (Waste Water); QJA9M5H4IM (Atrazine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


  5 / 40716 MEDLINE  
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[PMID]:28841524
[Au] Autor:Bharagava RN; Mishra S
[Ad] Endereço:Laboratory for Bioremediation and Metagenomics Research (LBMR), Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow 226025, U.P., India. Electronic address: bharagavarnbbau11@gmail.com.
[Ti] Título:Hexavalent chromium reduction potential of Cellulosimicrobium sp. isolated from common effluent treatment plant of tannery industries.
[So] Source:Ecotoxicol Environ Saf;147:102-109, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Present study deals with the isolation and characterization of a bacterium capable for the effective reduction of Cr(VI) from tannery wastewater. Based on the 16S rRNA gene sequence analysis, this bacterium was identified as Cellulosimicrobium sp. (KX710177). During the Cr(VI) reduction experiment performed at 50, 100, 200,and 300mg/L of Cr(VI) concentrations, the bacterium showed 99.33% and 96.98% reduction at 50 and 100mg/L at 24 and 96h, respectively. However, at 200 and 300mg/L concentration of Cr(VI), only 84.62% and 62.28% reduction was achieved after 96h, respectively. The SEM analysis revealed that bacterial cells exposed to Cr(VI) showed increased cell size in comparison to unexposed cells, which might be due to either the precipitation or adsorption of reduced Cr(III) on bacterial cells. Further, the Energy Dispersive X-ray (EDX) analysis showed some chromium peaks for cells exposed to Cr(VI), which might be either due to the presence of precipitated reduced Cr(III) on cells or complexation of Cr(III) with cell surface molecules. The bacterium also showed resistance and sensitivity against the tested antibiotics with a wide range of MIC values ranging from 250 to 800mg/L for different heavy metals. Thus, this multi-drug and multi-metal resistant bacterium can be used as a potential agent for the effective bioremediation of metal contaminated sites.
[Mh] Termos MeSH primário: Cromo/análise
Micrococcaceae/isolamento & purificação
Curtume
Águas Residuais/microbiologia
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cromo/química
Cromo/toxicidade
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Micrococcaceae/efeitos dos fármacos
Micrococcaceae/genética
Oxirredução
RNA Ribossômico 16S/genética
Águas Residuais/análise
Poluentes Químicos da Água/química
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Waste Water); 0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium); 18540-29-9 (chromium hexavalent ion)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


  6 / 40716 MEDLINE  
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[PMID]:29458668
[Au] Autor:Al-Ahmad A; Muzafferiy F; Anderson AC; Wölber JP; Ratka-Krüger P; Fretwurst T; Nelson K; Vach K; Hellwig E
[Ad] Endereço:1​Department of Operative Dentistry and Periodontology, Faculty of Medicine, Medical Center - University of Freiburg, Germany.
[Ti] Título:Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning.
[So] Source:J Med Microbiol;67(3):332-340, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites. METHODOLOGY: Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers. After cloning the PCR-products, amplified inserts of positive clones were digested using restriction endonucleases, and the chosen clones were sequenced. The 16S rDNA-sequences were compared to those from the public sequence databases GenBank, EMBL and DDBJ to determine the corresponding taxa. RESULTS: Differing distributions of taxa belonging to the phyla Firmicutes, Bacteroidetes, Fusobacteria, Actinobacteria, Proteobacteria, Synergistetes, Spirochaetae and TM 7 were detected in both the healthy implant (HI) and the peri-implantitis (PI) groups. A significantly higher relative abundance of phylum Bacteroidetes, as well as of the species Fusobacterium nucleatum, were found in the PI group (P<0.05). The putative periodontal red complex (Porphyromonas gingivalis, Tannerella forsythia) was also detected at significantly higher levels in the PI group (P<0.05), whereas the yellow group, as well as the species Veillonella dispar, tended to be associated with the HI group. CONCLUSION: A shift in the healthy subgingival microbiota was shown in peri-implantitis-associated biofilm. Anaerobic Gram-negative periopathogens, including P. gingivalis and T. forsythia, seem to play an important role in peri-implantitis development and should be considered in treatment and prevention strategies.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Biofilmes
Microbiota/genética
Peri-Implantite/microbiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Bactérias/classificação
Bactérias/genética
Carga Bacteriana
Fenômenos Fisiológicos Bacterianos
Bacteroides/genética
Bacteroides/isolamento & purificação
Feminino
Fusobacterium nucleatum/genética
Fusobacterium nucleatum/isolamento & purificação
Genes de RNAr
Gengiva/microbiologia
Seres Humanos
Masculino
Meia-Idade
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/isolamento & purificação
Prevotella intermedia/genética
Prevotella intermedia/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000682


  7 / 40716 MEDLINE  
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[PMID]:29458666
[Au] Autor:Yang ZW; Salam N; Asem MD; Fang BZ; Lan L; Xiao M; Wadaan MAM; Hozzein WN; Li WJ
[Ad] Endereço:1​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
[Ti] Título:Saccharopolyspora deserti sp. nov., a novel halotolerant actinobacterium isolated from a desert.
[So] Source:Int J Syst Evol Microbiol;68(3):860-864, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain SYSU D8010 was isolated from a desert sand sample collected in Saudi Arabia. The taxonomic position of the isolate was investigated by the polyphasic taxonomic approach. The isolate was found to be Gram-positive and aerobic. The strain was able to grow at 14-40 °C, pH 5.0-9.0 and in the presence of up to 22 % (w/v) NaCl. Strain SYSU D8010 contained meso-diaminopimelic acid as cell-wall diamino acid, and arabinose, fucose, galactose, glucose and rhamnose as the whole-cell sugars. The primary polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannosides. Menaquinone MK-9(H4) was detected as the respiratory quinone; and anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C17 : 0 as the predominant fatty acids. Pairwise comparison of the 16S rRNA gene sequences indicated that strain SYSU D8010 had a sequence similarity of 97.8 % to Saccharopolyspora halophila YIM 90500 . The genomic DNA G+C content of strain SYSU D8010 was determined to be 69.9 mol%. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU D8010 was determined to represent a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora deserti sp. nov. is proposed. The type strain of the species is SYSU D8010 (=KCTC 39989 =CPCC 204620 ).
[Mh] Termos MeSH primário: Clima Desértico
Filogenia
Saccharopolyspora/classificação
Salinidade
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Saccharopolyspora/genética
Saccharopolyspora/isolamento & purificação
Arábia Saudita
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002598


  8 / 40716 MEDLINE  
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[PMID]:29458662
[Au] Autor:Li W; Ten LN; Lee SY; Kang IK; Jung HY
[Ad] Endereço:1​School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
[Ti] Título:Spirosoma horti sp. nov., isolated from apple orchard soil.
[So] Source:Int J Syst Evol Microbiol;68(3):930-935, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-negative, motile by gliding, rod-shaped, aerobic bacterium, designated S7-3-19 , was isolated from apple orchard soil in Gyeongsangnam-do Province, Republic of Korea, and characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain S7-3-19 belonged to the family Cytophagaceae and was most closely related to Spirosoma linguale DSM 74 (96.38 %), Spirosoma fluviale MSd3 (96.38 %), Spirosoma pulveris JSH5-14 (96.35 %) and Spirosoma radiotolerans DG5A (96.24 %). Chemotaxonomic characteristics supported the classification of strain S7-3-19 within the genus Spirosoma. Summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 46.7 %) and C16 : 1ω5c (23.8 %) were the major fatty acids. Phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid and two unidentified lipids were the major polar lipids. Menaquinone with seven isoprene units was the predominant respiratory quinone. The G+C content of the genomic DNA of strain S7-3-19 was 48.6 mol%. On the basis of its phenotypic properties, genotypic distinctiveness and chemotaxonomic features, strain S7-3-19 represents a novel species of the genus Spirosoma, for which the name Spirosomahorti sp. nov. is proposed. The type strain is S7-3-19 (=KCTC 52728 =JCM 32131 ).
[Mh] Termos MeSH primário: Cytophagaceae/classificação
Fazendas
Malus
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Cytophagaceae/genética
Cytophagaceae/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Fosfatidiletanolaminas/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 39382-08-6 (phosphatidylethanolamine); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002614


  9 / 40716 MEDLINE  
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[PMID]:29458566
[Au] Autor:Kim YS; Cha CJ
[Ad] Endereço:Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea.
[Ti] Título:Paenibacillus translucens sp. nov., isolated from tidal flat sediment.
[So] Source:Int J Syst Evol Microbiol;68(3):936-941, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11 , was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11 grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11 possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11 belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691 (99.36-99.15 % similarity). DNA-DNA relatedness levels of strain CJ11 was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691 . The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11 contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11 represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp. nov. is proposed. The type strain is CJ11 (=KACC 19304 =JCM 32080 ).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Paenibacillus/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Glicolipídeos/química
Paenibacillus/genética
Paenibacillus/isolamento & purificação
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002613


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[PMID]:29458546
[Au] Autor:Cui MD; Wang X; Jiang WK; Hu G; Yang ZG; Sun GJ; Zhu SJ; Zhou YD; Hong Q
[Ad] Endereço:1​Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
[Ti] Título:Pedobacter agrisoli sp. nov., isolated from farmland soil.
[So] Source:Int J Syst Evol Microbiol;68(3):886-891, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, designated YHM-9 , was isolated from soil in Yangquan, Shanxi Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YHM-9 belonged to the genus Pedobacter and shared the highest similarity (97.4 %) to the type strain Pedobacter lignilitoris W-WS13 . Strain YHM-9 exhibited low DNA-DNA relatedness with P. lignilitoris W-WS13 (21.7±1.3 %). The DNA G+C content was 38.9 mol%. The major fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH. The respiratory quinone was MK-7, the major polyamine was sym-homospermidine and the major polar lipids were phosphatidylethanolamine. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics, strain YHM-9 was recognized as a representative of a novel species within the genus Pedobacter, for which the name Pedobacteragrisoli sp. nov. is proposed. The type strain is YHM-9 (=JCM 32093 =CCTCC AB 2017125 ).
[Mh] Termos MeSH primário: Fazendas
Pedobacter/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Pedobacter/genética
Pedobacter/isolamento & purificação
Fosfatidiletanolaminas/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espermidina/análogos & derivados
Espermidina/química
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (RNA, Ribosomal, 16S); 0IJ25X1H4R (sym-homospermidine); 11032-49-8 (Vitamin K 2); 39382-08-6 (phosphatidylethanolamine); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002604



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