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  1 / 3049 MEDLINE  
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[PMID]:28990461
[Au] Autor:Oikawa R; Watanabe Y; Miyamoto S; Sato Y; Ono S; Mabe K; Yamamoto H; Kato M; Itoh F
[Ad] Endereço:1 Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan.
[Ti] Título:Enrichment of Helicobacter pylori mutant strains after eradication therapy analyzed by gastric wash-based quantitative pyrosequencing.
[So] Source:Tumour Biol;39(10):1010428317734865, 2017 Oct.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The eradication of Helicobacter pylori reduces the risk of gastric cancer. A clear understanding of the factors underlying mixed infection with multiple clarithromycin-susceptible and clarithromycin-resistant H. pylori strains is necessary to design more effective therapies against H. pylori. We aimed to assess how the abundance and prevalence of H. pylori strains vary after clarithromycin-based eradication therapy. Using gastric wash samples, which represent the entire stomach, we sequentially analyzed the abundance and prevalence of H. pylori DNA by 23S ribosomal RNA pyrosequencing before and 1, 2, and 3 years after eradication therapy. Low levels of H. pylori DNA were still detectable at the first-year follow-up in all samples with negative post-treatment urea breath test results. The abundance of H. pylori DNA decreased significantly until the 2-year follow-up, but it switched to an increase at the 3-year follow-up. Importantly, the ratio of the prevalence of mutant strains to the prevalence of wild-type strains had already increased at the first-year follow-up and continued to increase, suggesting the selection and growth of clarithromycin-resistant strains during the follow-up periods. Being sensitive and representative, our assay will be useful in effectively addressing gastric cancer development by enhancing the long-term success of intervention strategies and consecutive surveillance for H. pylori eradication.
[Mh] Termos MeSH primário: Infecções por Helicobacter/microbiologia
Helicobacter pylori/genética
Neoplasias Gástricas/microbiologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Antibacterianos/uso terapêutico
Líquidos Corporais/microbiologia
Claritromicina/uso terapêutico
DNA Bacteriano/análise
Feminino
Seguimentos
Infecções por Helicobacter/tratamento farmacológico
Seres Humanos
Masculino
Meia-Idade
RNA Ribossômico 23S/análise
Reação em Cadeia da Polimerase em Tempo Real
Irrigação Terapêutica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 23S); H1250JIK0A (Clarithromycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317734865


  2 / 3049 MEDLINE  
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[PMID]:28950002
[Au] Autor:Mitura A; Niemczuk K; Zareba K; Zajac M; Laroucau K; Szymanska-Czerwinska M
[Ad] Endereço:Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Pulawy, Poland.
[Ti] Título:Free-living and captive turtles and tortoises as carriers of new Chlamydia spp.
[So] Source:PLoS One;12(9):e0185407, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A variety of Chlamydia species belonging to the Chlamydiaceae family have been reported in reptilian hosts but scarce data about their occurrence in turtles and tortoises are available. In this study, research was conducted to acquire information on invasive alien species (IAS) of turtles and indigenous turtles and tortoises, living both free and in captivity, as possible reservoirs of Chlamydiaceae. Analysis of specimens (pharyngeal and cloacal swabs and tissues) from 204 turtles and tortoises revealed an overall Chlamydiaceae prevalence of 18.3% and 28.6% among free-living and captive animals respectively, with variable levels of shedding. Further testing conducted with a species-specific real-time PCR and microarray test was unsuccessful. Subsequently sequencing was applied to genotype the Chlamydiaceae-positive samples. Almost the full lengths of the 16S rRNA and ompA genes as well as the 16S-23S intergenic spacer (IGS) and 23S rRNA domain I were obtained for 14, 20 and 8 specimens respectively. Phylogenetic analysis of 16S rRNA amplicons revealed two distinct branches. Group 1 (10 specimens), specific to freshwater turtles and reported here for the first time, was most closely related to Chlamydia (C.) pneumoniae strains and the newly described Candidatus C. sanzinia. Group 2 (four specimens), detected in Testudo spp. samples, showed highest homology to C. pecorum strains but formed a separate sub-branch. Finally, molecular analysis conducted on positive samples together with their geographical distribution in places distant from each other strongly suggest that Group 1 specimens correspond to a new species in the Chlamydiaceae family. In-depth studies of Chlamydia spp. from turtles and tortoises are needed to further characterise these atypical strains and address arising questions about their pathogenicity and zoonotic potential.
[Mh] Termos MeSH primário: Chlamydia/isolamento & purificação
Tartarugas/microbiologia
[Mh] Termos MeSH secundário: Animais
Chlamydia/classificação
Chlamydia/genética
Genótipo
Filogenia
RNA Ribossômico 16S/genética
RNA Ribossômico 23S/genética
Reação em Cadeia da Polimerase em Tempo Real
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185407


  3 / 3049 MEDLINE  
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[PMID]:28941460
[Au] Autor:Makarov GI; Sumbatyan NV; Bogdanov AA
[Ad] Endereço:Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119991, Russia.
[Ti] Título:Structural Insight into Interaction between C20 Phenylalanyl Derivative of Tylosin and Ribosomal Tunnel.
[So] Source:Biochemistry (Mosc);82(8):925-932, 2017 Aug.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Macrolides are clinically important antibiotics that inhibit protein biosynthesis on ribosomes by binding to ribosomal tunnel. Tylosin belongs to the group of 16-membered macrolides. It is a potent inhibitor of translation whose activity is largely due to reversible covalent binding of its aldehyde group with the base of A2062 in 23S ribosomal RNA. It is known that the conversion of the aldehyde group of tylosin to methyl or carbinol groups dramatically reduces its inhibitory activity. However, earlier we obtained several derivatives of tylosin having comparable activity in spite of the fact that the aldehyde group of tylosin in these compounds was substituted with an amino acid or a peptide residue. Details of the interaction of these compounds with the ribosome that underlies their high inhibitory activity were not known. In the present work, the structure of the complex of tylosin derivative containing in position 20 the residue of ethyl ester of 2-imino(oxy)acetylphenylalanine with the tunnel of the E. coli ribosome was identified by means of molecular dynamics simulations, which could explain high biological activity of this compound.
[Mh] Termos MeSH primário: RNA Ribossômico 23S/metabolismo
Tilosina/metabolismo
[Mh] Termos MeSH secundário: Sítios de Ligação
Escherichia coli/metabolismo
Ligações de Hidrogênio
Simulação de Dinâmica Molecular
Fenilalanina/química
Estrutura Terciária de Proteína
RNA Ribossômico 23S/química
Tilosina/análogos & derivados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 23S); 47E5O17Y3R (Phenylalanine); YEF4JXN031 (Tylosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917080077


  4 / 3049 MEDLINE  
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[PMID]:28786485
[Au] Autor:Agmon I
[Ad] Endereço:Institute for Advanced Studies in Theoretical Chemistry, Schulich Faculty of Chemistry, Technion - Israel Institute of Technology, Haifa, Israel.
[Ti] Título:Sequence complementarity at the ribosomal Peptidyl Transferase Centre implies self-replicating origin.
[So] Source:FEBS Lett;591(20):3252-3258, 2017 Oct.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A feasible scenario for the emergence of life requires the spontaneous materialization and sustainability of a proto-ribosome that could have catalysed the formation of the first peptides. Models of proto-ribosomes were derived from the ribosomal Peptidyl Transferase Centre (PTC) region, but the poor prebiotic copying abilities give rise to the question of their mode of replication. Here, complementarity is demonstrated in bacterial ribosomes, between nucleotides that constitute the two halves of the PTC cavity. The complementarity corroborates the dimeric nature of the proto-ribosome and is likely to underlie the symmetry of the PTC region. Furthermore, it indicates a simple and efficient replication mode; the strand of each monomer could have acted as a template for the synthesis of its counterpart, forming a self-replicating ribozyme.
[Mh] Termos MeSH primário: Origem da Vida
Peptidil Transferases/química
RNA Catalítico/química
RNA Ribossômico 23S/química
Ribossomos/metabolismo
[Mh] Termos MeSH secundário: Pareamento de Bases
Biocatálise
Escherichia coli/genética
Escherichia coli/metabolismo
Evolução Molecular
Modelos Biológicos
Modelos Moleculares
Conformação de Ácido Nucleico
Peptidil Transferases/genética
Peptidil Transferases/metabolismo
RNA Catalítico/genética
RNA Catalítico/metabolismo
RNA Ribossômico 23S/genética
RNA Ribossômico 23S/metabolismo
Ribossomos/genética
Ribossomos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Catalytic); 0 (RNA, Ribosomal, 23S); EC 2.3.2.12 (Peptidyl Transferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12781


  5 / 3049 MEDLINE  
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[PMID]:28768558
[Au] Autor:Li L; Luther M; Macklin K; Pugh D; Li J; Zhang J; Roberts J; Kaltenboeck B; Wang C
[Ad] Endereço:Thompson Bishop Sparks State Diagnostic Laboratory,Auburn, AL,USA.
[Ti] Título:Chlamydia gallinacea: a widespread emerging Chlamydia agent with zoonotic potential in backyard poultry.
[So] Source:Epidemiol Infect;145(13):2701-2703, 2017 10.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chlamydia gallinacea, a new chlamydial agent, has been reported in four European countries as well as Argentina and China. Experimentally infected chickens with C. gallinacea in previous study showed no clinical signs but had significantly reduced gains in body weight (6·5-11·4%). Slaughterhouse workers exposed to infected chickens have developed atypical pneumonia, indicating C. gallinacea is likely a zoonotic agent. In this study, FRET-PCR confirmed that C. gallinacea was present in 12·4% (66/531) of oral-pharyngeal samples from Alabama backyard poultry. Phylogenetic comparisons based on ompA variable domain showed that 16 sequenced samples represented 14 biotypes. We report for the first time the presence of C. gallinacea in North America, and this warrants further research on the organism's pathogenicity, hosts, transmission, and zoonotic potential.
[Mh] Termos MeSH primário: Galinhas
Infecções por Chlamydia/veterinária
Chlamydia/isolamento & purificação
Doenças Transmissíveis Emergentes/veterinária
Doenças das Aves Domésticas/epidemiologia
[Mh] Termos MeSH secundário: Alabama/epidemiologia
Animais
Chlamydia/genética
Infecções por Chlamydia/epidemiologia
Infecções por Chlamydia/microbiologia
Doenças Transmissíveis Emergentes/epidemiologia
Doenças Transmissíveis Emergentes/microbiologia
Reação em Cadeia da Polimerase/veterinária
Doenças das Aves Domésticas/microbiologia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
RNA Ribossômico 23S/genética
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817001650


  6 / 3049 MEDLINE  
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[PMID]:28629504
[Au] Autor:Sakai HD; Kurosawa N
[Ad] Endereço:Department of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577, Japan.
[Ti] Título:Sulfodiicoccus acidiphilus gen. nov., sp. nov., a sulfur-inhibited thermoacidophilic archaeon belonging to the order Sulfolobales isolated from a terrestrial acidic hot spring.
[So] Source:Int J Syst Evol Microbiol;67(6):1880-1886, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel thermoacidophilic archaeon, strain HS-1T, was isolated from the Hakone Ohwaku-dani hot spring in Japan. Cells of strain HS-1T in exponential phase were cocci to irregular cocci with a diameter of 0.8-1.5 µm. The strain grew within a temperature range of 50-70 °C (optimal: 65-70 °C), a pH range of pH 1.4-5.5 (optimal: pH 3.0-3.5) and a NaCl concentration range of 0-2.5 % (w/v). The novel strain grew in aerobic conditions but did not grow anaerobically. Moreover, this strain utilized various complex substrates (beef extract, casamino acids, peptone, tryptone and yeast extract) and sugars (arabinose, xylose, galactose, glucose, maltose, sucrose, raffinose and lactose) as sole carbon sources. No chemolithoautotrophic growth occurred on elemental sulfur, pyrite, K2S4O6, Na2S2O3 or FeSO4 . 7H2O; however, growth by the oxidation of hydrogen occurred weakly. The core lipids were calditoglycerocaldarchaeol (CGTE) and caldarchaeol (DGTE). The DNA G+C content of the strain was 52.0 mol%, which was remarkably higher than those of known species of the order Sulfolobales(31-46.2 %). The growth of the strain was significantly inhibited in the presence of elemental sulfur. Analyses of 16S rRNA and 23S rRNA gene sequences showed that HS-1T belonged to the order Sulfolobales; however, it was distantly related to all known species of the order Sulfolobales (less than 89 % sequence similarity). On the basis of these results, we propose the novel genus, Sulfodiicoccus, in the order Sulfolobales (in the family Sulfolobaceae). The type species of the genus is Sulfodiicoccus acidiphilus sp. nov., and the type strain of the species is HS-1T (=JCM 31740T=InaCC Ar79T).
[Mh] Termos MeSH primário: Fontes Termais/microbiologia
Filogenia
Sulfolobaceae/classificação
[Mh] Termos MeSH secundário: Crescimento Quimioautotrófico
DNA Arqueal/genética
Temperatura Alta
Japão
Lipídeos/química
RNA Ribossômico 16S/genética
RNA Ribossômico 23S/genética
Análise de Sequência de DNA
Sulfolobaceae/genética
Sulfolobaceae/isolamento & purificação
Enxofre
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Lipids); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S); 70FD1KFU70 (Sulfur)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001881


  7 / 3049 MEDLINE  
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[PMID]:28576826
[Au] Autor:Tomkuviene M; Licyte J; Olendraite I; Liutkeviciute Z; Clouet-d'Orval B; Klimasauskas S
[Ad] Endereço:Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University, Vilnius LT-10257, Lithuania.
[Ti] Título:Archaeal fibrillarin-Nop5 heterodimer 2'- -methylates RNA independently of the C/D guide RNP particle.
[So] Source:RNA;23(9):1329-1337, 2017 Sep.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Archaeal fibrillarin (aFib) is a well-characterized -adenosyl methionine (SAM)-dependent RNA 2'- -methyltransferase that is known to act in a large C/D ribonucleoprotein (RNP) complex together with Nop5 and L7Ae proteins and a box C/D guide RNA. In the reaction, the guide RNA serves to direct the methylation reaction to a specific site in tRNA or rRNA by sequence complementarity. Here we show that a aFib-Nop5 heterodimer can alone perform SAM-dependent 2'- -methylation of 16S and 23S ribosomal RNAs in vitro independently of L7Ae and C/D guide RNAs. Using tritium-labeling, mass spectrometry, and reverse transcription analysis, we identified three in vitro 2'- -methylated positions in the 16S rRNA of , positions lying outside of previously reported pyrococcal C/D RNP methylation sites. This newly discovered stand-alone activity of aFib-Nop5 may provide an example of an ancestral activity retained in enzymes that were recruited to larger complexes during evolution.
[Mh] Termos MeSH primário: Archaea/genética
Archaea/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
RNA Arqueal/genética
RNA Arqueal/metabolismo
Ribonucleoproteínas Nucleolares Pequenas/metabolismo
Ribonucleoproteínas/metabolismo
[Mh] Termos MeSH secundário: Proteínas Cromossômicas não Histona/química
Metilação
Conformação de Ácido Nucleico
Ligação Proteica
Multimerização Proteica
RNA Ribossômico 16S/química
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
RNA Ribossômico 23S/química
RNA Ribossômico 23S/genética
RNA Ribossômico 23S/metabolismo
Ribonucleoproteínas/química
Ribonucleoproteínas Nucleolares Pequenas/química
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (RNA, Archaeal); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S); 0 (Ribonucleoproteins); 0 (Ribonucleoproteins, Small Nucleolar); 0 (fibrillarin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE
[do] DOI:10.1261/rna.059832.116


  8 / 3049 MEDLINE  
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[PMID]:28505372
[Au] Autor:Osterman IA; Khabibullina NF; Komarova ES; Kasatsky P; Kartsev VG; Bogdanov AA; Dontsova OA; Konevega AL; Sergiev PV; Polikanov YS
[Ad] Endereço:Lomonosov Moscow State University, Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Moscow 119992, Russia.
[Ti] Título:Madumycin II inhibits peptide bond formation by forcing the peptidyl transferase center into an inactive state.
[So] Source:Nucleic Acids Res;45(12):7507-7514, 2017 Jul 07.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The emergence of multi-drug resistant bacteria is limiting the effectiveness of commonly used antibiotics, which spurs a renewed interest in revisiting older and poorly studied drugs. Streptogramins A is a class of protein synthesis inhibitors that target the peptidyl transferase center (PTC) on the large subunit of the ribosome. In this work, we have revealed the mode of action of the PTC inhibitor madumycin II, an alanine-containing streptogramin A antibiotic, in the context of a functional 70S ribosome containing tRNA substrates. Madumycin II inhibits the ribosome prior to the first cycle of peptide bond formation. It allows binding of the tRNAs to the ribosomal A and P sites, but prevents correct positioning of their CCA-ends into the PTC thus making peptide bond formation impossible. We also revealed a previously unseen drug-induced rearrangement of nucleotides U2506 and U2585 of the 23S rRNA resulting in the formation of the U2506•G2583 wobble pair that was attributed to a catalytically inactive state of the PTC. The structural and biochemical data reported here expand our knowledge on the fundamental mechanisms by which peptidyl transferase inhibitors modulate the catalytic activity of the ribosome.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/antagonistas & inibidores
Peptidil Transferases/antagonistas & inibidores
Inibidores da Síntese de Proteínas/farmacologia
RNA de Transferência/antagonistas & inibidores
Ribossomos/efeitos dos fármacos
Estreptograminas/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Domínio Catalítico
Escherichia coli/efeitos dos fármacos
Escherichia coli/enzimologia
Escherichia coli/genética
Modelos Moleculares
Conformação de Ácido Nucleico
Peptidil Transferases/química
Peptidil Transferases/genética
Peptidil Transferases/metabolismo
Biossíntese de Proteínas/efeitos dos fármacos
Inibidores da Síntese de Proteínas/química
RNA Ribossômico 23S/antagonistas & inibidores
RNA Ribossômico 23S/química
RNA Ribossômico 23S/metabolismo
RNA de Transferência/química
RNA de Transferência/metabolismo
Ribossomos/genética
Ribossomos/metabolismo
Estreptograminas/química
Thermus thermophilus/efeitos dos fármacos
Thermus thermophilus/enzimologia
Thermus thermophilus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Protein Synthesis Inhibitors); 0 (RNA, Ribosomal, 23S); 0 (Streptogramins); 0 (madumycin II); 9014-25-9 (RNA, Transfer); EC 2.3.2.12 (Peptidyl Transferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx413


  9 / 3049 MEDLINE  
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[PMID]:28504455
[Au] Autor:Sato T; Higuchi H; Yokota SI; Tamura Y
[Ad] Endereço:Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyoudai-Midorimachi, Ebetsu, 069-8501, Japan.
[Ti] Título:Mycoplasma bovis isolates from dairy calves in Japan have less susceptibility than a reference strain to all approved macrolides associated with a point mutation (G748A) combined with multiple species-specific nucleotide alterations in 23S rRNA.
[So] Source:Microbiol Immunol;61(6):215-224, 2017 Jun.
[Is] ISSN:1348-0421
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Erythromycin, tylosin and tilmicosin are approved for use in cattle in Japan, the latter two being used to treat Mycoplasma bovis infection. In this study, 58 M. bovis isolates obtained from Japanese dairy calves all exhibited reduced susceptibility to these macrolides, this widespread reduced susceptibility being attributable to a few dominant lineages. All 58 isolates contained the G748A variant in both the rrl3 and rrl4 alleles of 23S rRNA, whereas a reference strain (PG45) did not. G748 localizes in the central loop of domain II (from C744 to A753) of 23S rRNA, which participates in binding to mycinose, a sugar residue present in both tylosin and tilmicosin. A number of in vitro-selected mutants derived from M. bovis PG45 showed reduced susceptibility to tylosin and tilmicosin and contained a nucleotide insertion within the central loop of domain II of rrl3 (U747-G748Ins_CU/GU or A743-U744Ins_UA), suggesting that mutations around G748 confer this reduced susceptibility phenotype. However, other Mycoplasma species containing G748A were susceptible to tylosin and tilmicosin. Sequence comparison with Escherichia coli revealed that M. bovis PG45 and isolates harbored five nucleotide alterations (U744C, G745A, U746C, A752C and A753G) in the central loop of domain II of 23S rRNA, whereas other Mycoplasma species lacked at least two of these five nucleotide alterations. It was therefore concluded that G748 mutations in combination with species-specific nucleotide alterations in the central loop of domain II of 23S rRNA are likely sufficient to reduce susceptibility of M. bovis to tylosin and tilmicosin.
[Mh] Termos MeSH primário: Laticínios/microbiologia
Macrolídeos/farmacologia
Mycoplasma bovis/efeitos dos fármacos
Mycoplasma bovis/genética
Mycoplasma bovis/isolamento & purificação
Mutação Puntual
RNA Ribossômico 23S/genética
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Bovinos
DNA Bacteriano/genética
Farmacorresistência Bacteriana/genética
Eritromicina/farmacologia
Escherichia coli/genética
Genes Bacterianos/genética
Genótipo
Técnicas de Genotipagem
Japão
Testes de Sensibilidade Microbiana
Mutagênese Insercional
Infecções por Mycoplasma/veterinária
Especificidade da Espécie
Tilosina/análogos & derivados
Tilosina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Macrolides); 0 (RNA, Ribosomal, 23S); 63937KV33D (Erythromycin); XL4103X2E3 (tilmicosin); YEF4JXN031 (Tylosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1111/1348-0421.12490


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[PMID]:28407014
[Au] Autor:Shipitsyna E; Rumyantseva T; Golparian D; Khayrullina G; Lagos AC; Edelstein I; Joers K; Jensen JS; Savicheva A; Rudneva N; Sukhanova L; Kozlov R; Guschin A; Unemo M
[Ad] Endereço:Laboratory of Microbiology, D.O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology, St. Petersburg, Russia.
[Ti] Título:Prevalence of macrolide and fluoroquinolone resistance-mediating mutations in Mycoplasma genitalium in five cities in Russia and Estonia.
[So] Source:PLoS One;12(4):e0175763, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVE: Resistance in the sexually transmitted bacterium Mycoplasma genitalium to all recommended therapeutic antimicrobials have rapidly emerged. However, to date, internationally reported resistance surveillance data for M. genitalium strains circulating in Eastern Europe are entirely lacking. The aim of this study was to estimate the prevalence of macrolide and fluoroquinolone resistance-associated mutations in M. genitalium in four cities in Russia and one in Estonia, 2013-2016. MATERIALS AND METHODS: Consecutive urogenital samples found positive for M. genitalium during diagnostic testing were retrospectively analyzed for resistance-associated mutations in the 23S rRNA and parC genes using pyrosequencing and conventional Sanger sequencing, respectively. RESULTS: In total, 867 M. genitalium positive samples from 2013-2016 were analyzed. Macrolide resistance-associated mutations were detected in 4.6% of the samples from Russia (0.7-6.8% in different cities) and in 10% of the samples from Estonia. The mutations A2059G and A2058G were highly predominating in both Russia and Estonia, accounting together for 90.9% of the cases positive for nucleotide substitutions in the 23S rRNA gene. The rates of possible fluoroquinolone resistance-associated mutations were 6.2% in Russia (2.5-7.6% in different cities) and 5% in Estonia. The mutations S83I and S83N were the most frequent ones in Russia (24.4% each), whereas D87N highly predominated in Estonia (83.3% of all fluoroquinolone resistance-associated mutations). Approximately 1% of the samples in both countries harbored both macrolide and possible fluoroquinolone resistance-associated mutations, with A2058G and S83I being the most frequent combination (37.5%). CONCLUSIONS: The prevalence of macrolide and fluoroquinolone resistance-associated mutations in M. genitalium was 4.6% and 6.2%, respectively, in Russia, and 10% and 5%, respectively, in Estonia. Despite the relatively low rates of macrolide and fluoroquinolone resistance in these countries, antimicrobial resistance surveillance and testing for resistance-associated mutations in M. genitalium positive cases would be valuable.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana
Fluoroquinolonas/farmacologia
Macrolídeos/farmacologia
Mutação
Infecções por Mycoplasma/microbiologia
Mycoplasma genitalium/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/análise
Estônia
Feminino
Fluoroquinolonas/uso terapêutico
Seres Humanos
Macrolídeos/uso terapêutico
Masculino
Mycoplasma genitalium/isolamento & purificação
Vigilância da População
Prevalência
RNA Ribossômico 23S/análise
Federação Russa
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fluoroquinolones); 0 (Macrolides); 0 (RNA, Ribosomal, 23S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175763



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