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[PMID]: | 28456667 |
[Au] Autor: | Chantratita W; Song KS; Nimse SB; Pongthanapisith V; Thongbaiphet N; Wongtabtim G; Pasomsub E; Angkanavin K; Sonawane MD; Warkad SD; Kim T |
[Ad] Endereço: | Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: wasun.cha@mahidol.ac.th. |
[Ti] Título: | 6 HCV Genotyping 9G test for HCV 1a, 1b, 2, 3, 4 and 6 (6a, 6f, 6i and 6n) with high accuracy. |
[So] Source: | J Virol Methods;246:95-99, 2017 Aug. | [Is] ISSN: | 1879-0984 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | According to EASL guidelines and WHO recommendations, the accurate detection of HCV genotypes such as HCV 1a, HCV1b, HCV 2, HCV 3, HCV 4, and HCV 6 (6a, 6f, 6i, 6n) is crucial for the efficient treatment of hepatitis C. HCV Genotyping 9G test allows simultaneous genotyping of HCV 1a, 1b, 2, 3, 4, and 6 (6a, 6f, 6i, and 6n) in clinical samples in 30min. The performance of the test was evaluated by comparison with sequence analysis. Serum samples (n=152) from HCV-infected patients (n=110) and healthy individuals (n=42) were processed under blinded codes. The k coefficient (kappa) values indicated high agreement between the HCV Genotyping 9G test and sequencing. The sensitivity and specificity of the test were 99.1% and 99.7%, respectively. The results indicate that HCV Genotyping 9G test is rapid, reliable, sensitive, and accurate for screening and genotyping of HCV in the clinical specimens. |
[Mh] Termos MeSH primário: |
Técnicas de Genotipagem/métodos Hepacivirus/genética
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[Mh] Termos MeSH secundário: |
Primers do DNA Genótipo Hepacivirus/classificação Seres Humanos Cirrose Hepática/virologia Reação em Cadeia da Polimerase/métodos Sensibilidade e Especificidade Análise de Sequência de DNA Proteínas não Estruturais Virais/genética
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA Primers); 0 (Viral Nonstructural Proteins) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180226 |
[Lr] Data última revisão:
| 180226 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170501 |
[St] Status: | MEDLINE |
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