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Pesquisa : D13.695.578.424.550 [Categoria DeCS]
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  1 / 2038 MEDLINE  
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[PMID]:29211451
[Au] Autor:Soudah T; Mogilevsky M; Karni R; Yavin E
[Ad] Endereço:The Institute for Drug Research, The School of Pharmacy and ‡Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, The Faculty of Medicine, The Hebrew University of Jerusalem , Hadassah Ein-Kerem, Jerusalem 91120, Israel.
[Ti] Título:CLIP6-PNA-Peptide Conjugates: Non-Endosomal Delivery of Splice Switching Oligonucleotides.
[So] Source:Bioconjug Chem;28(12):3036-3042, 2017 Dec 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Efficient delivery of oligonucleotides still remains a challenge in the field of oligonucleotide based therapy. Peptide nucleic acid (PNA), a DNA analogue that is typically synthesized by solid phase peptide chemistry, has been conjugated to a variety of cell penetrating peptides (CPP) as a means of improving its cellular uptake. These CPPs typically deliver their cargoes into cells by an endosomal-dependent mechanism resulting in lower bioavailability of the cargo. Herein, we designed and synthesized PNA-peptide conjugates as splice switching oligonucleotides (SSO) targeting the Mnk2 gene, a therapeutic target in cancer. In humans, the MKNK2 gene, is alternatively spliced, generating isoforms with opposite biological activities: Mnk2a and Mnk2b. It was found that the Mnk2a isoform is down-regulated in breast, lung, brain, and colon tumors and is a tumor suppressor, whereas MnK2b is oncogenic. We have designed and synthesized PNAs that were conjugated to either of the following peptides: a nuclear localization sequence (NLS) or a cytosol localizing internalization peptide (CLIP6). CLIP6-PNA demonstrates effective cellular uptake and exclusively employs a nonendosomal mechanism to cross the cellular membranes of glioblastoma cells (U87). Simple incubation of PNA-peptide conjugates in human glioblastoma cells up-regulates the Mnk2a isoform leading to cancer cell death.
[Mh] Termos MeSH primário: Peptídeos Penetradores de Células/metabolismo
Portadores de Fármacos/metabolismo
Oligonucleotídeos/metabolismo
Ácidos Nucleicos Peptídicos/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Linhagem Celular Tumoral
Células HeLa
Seres Humanos
Concentração de Íons de Hidrogênio
Oligonucleotídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell-Penetrating Peptides); 0 (Drug Carriers); 0 (Oligonucleotides); 0 (Peptide Nucleic Acids)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00638


  2 / 2038 MEDLINE  
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[PMID]:28919425
[Au] Autor:Piras L; Avitabile C; D'Andrea LD; Saviano M; Romanelli A
[Ad] Endereço:Institute of Crystallography (IC), CNR, Via Amendola 122/O, 70126, Bari, Italy.
[Ti] Título:Detection of oligonucleotides by PNA-peptide conjugates recognizing the biarsenical fluorescein complex FlAsH-EDT .
[So] Source:Biochem Biophys Res Commun;493(1):126-131, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We report the application of the arsenical complex FlAsH-EDT for the identification of oligonucleotide sequences. We designed PNA sequences conjugated to either a tetracysteine motif and to split tetracysteine sequences, that are recognized by FlAsH. The effect of conjugation of the PNA to the tetracysteine peptide and RNA hybridization on the fluorescence of the arsenical complex has been investigated. The reconstitution of the tetracysteine motif, starting from 15-mer PNAs conjugated to split tetracysteine sequences and hybridized to a complementary oligonucleotide was also explored.
[Mh] Termos MeSH primário: Fluoresceínas/química
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Análise de Sequência com Séries de Oligonucleotídeos/métodos
Compostos Organometálicos/química
Ácidos Nucleicos Peptídicos/genética
Espectrometria de Fluorescência/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein); 0 (Fluoresceins); 0 (Organometallic Compounds); 0 (Peptide Nucleic Acids)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE


  3 / 2038 MEDLINE  
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[PMID]:28817123
[Au] Autor:Sharifi-Sanjani M; Meeker AK; Mourkioti F
[Ad] Endereço:Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
[Ti] Título:Evaluation of telomere length in human cardiac tissues using cardiac quantitative FISH.
[So] Source:Nat Protoc;12(9):1855-1870, 2017 Sep.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Telomere length has been correlated with various diseases, including cardiovascular disease and cancer. The use of currently available telomere-length measurement techniques is often restricted by the requirement of a large amount of cells (Southern-based techniques) or the lack of information on individual cells or telomeres (PCR-based methods). Although several methods have been used to measure telomere length in tissues as a whole, the assessment of cell-type-specific telomere length provides valuable information on individual cell types. The development of fluorescence in situ hybridization (FISH) technologies enables the quantification of telomeres in individual chromosomes, but the use of these methods is dependent on the availability of isolated cells, which prevents their use with fixed archival samples. Here we describe an optimized quantitative FISH (Q-FISH) protocol for measuring telomere length that bypasses the previous limitations by avoiding contributions from undesired cell types. We have used this protocol on small paraffin-embedded cardiac-tissue samples. This protocol describes step-by-step procedures for tissue preparation, permeabilization, cardiac-tissue pretreatment and hybridization with a Cy3-labeled telomeric repeat complementing (CCCTAA) peptide nucleic acid (PNA) probe coupled with cardiac-specific antibody staining. We also describe how to quantify telomere length by means of the fluorescence intensity and area of each telomere within individual nuclei. This protocol provides comparative cell-type-specific telomere-length measurements in relatively small human cardiac samples and offers an attractive technique to test hypotheses implicating telomere length in various cardiac pathologies. The current protocol (from tissue collection to image procurement) takes ∼28 h along with three overnight incubations. We anticipate that the protocol could be easily adapted for use on different tissue types.
[Mh] Termos MeSH primário: Hibridização in Situ Fluorescente/métodos
Miocárdio/química
Telômero/química
[Mh] Termos MeSH secundário: Carbocianinas/química
Seres Humanos
Ácidos Nucleicos Peptídicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbocyanines); 0 (Peptide Nucleic Acids); 0 (cyanine dye 3)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.082


  4 / 2038 MEDLINE  
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[PMID]:28800650
[Au] Autor:Patel N; Miller D; Relhan N; Flynn HW
[Ad] Endereço:Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States.
[Ti] Título:Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Detection of Staphylococci From Endophthalmitis Isolates: A Proof-of-Concept Study.
[So] Source:Invest Ophthalmol Vis Sci;58(10):4307-4309, 2017 08 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Rapid identification of pathogens causing endophthalmitis may improve treatment outcomes through early administration of species-specific medication. The current study reports a new molecular application of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with Staphylococcus-specific molecular PNA probes for the potential rapid detection of common pathogens causing endophthalmitis. Methods: An experimental study was designed to evaluate the proof of concept at the microbiology laboratory of the Bascom Palmer Eye Institute. Stored culture-positive staphylococci endophthalmitis isolates obtained from prior vitreous samples (n = 15), along with broth as negative controls (n = 5) were used. Inoculum was prepared to a final concentration of 1 × 105 colony-forming units/mL to ensure that the isolates were viable. Smears of samples were fixed and hybridized using QuickFISH protocol with probes for Staphylococcus. Results: With PNA-FISH technique, Staphylococcus aureus was identified in 9 of 10 samples and coagulase-negative staphylococci were identified in 10 of 10 samples. Detection time was 20 minutes. Conclusions: This study serves a proof of concept using a new microbial detection system with FISH probes, and may have the potential for clinical use in the rapid and accurate identification of isolates from patients with endophthalmitis.
[Mh] Termos MeSH primário: Endoftalmite/diagnóstico
Infecções Oculares Bacterianas/diagnóstico
Hibridização in Situ Fluorescente
Ácidos Nucleicos Peptídicos/genética
Infecções Estafilocócicas/diagnóstico
Staphylococcus aureus/isolamento & purificação
[Mh] Termos MeSH secundário: Técnicas Bacteriológicas
Endoftalmite/microbiologia
Infecções Oculares Bacterianas/microbiologia
Seres Humanos
Microscopia de Fluorescência
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Nucleic Acids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-21535


  5 / 2038 MEDLINE  
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[PMID]:28704609
[Au] Autor:Pansuwan H; Ditmangklo B; Vilaivan C; Jiangchareon B; Pan-In P; Wanichwecharungruang S; Palaga T; Nuanyai T; Suparpprom C; Vilaivan T
[Ad] Endereço:Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Naresuan University , Ta-Po District, Muang, Phitsanulok 65000, Thailand.
[Ti] Título:Hydrophilic and Cell-Penetrable Pyrrolidinyl Peptide Nucleic Acid via Post-synthetic Modification with Hydrophilic Side Chains.
[So] Source:Bioconjug Chem;28(9):2284-2292, 2017 Sep 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose-phosphate was replaced by a peptide-like backbone. The absence of negative charge in the PNA backbone leads to several unique behaviors including a stronger binding and salt independency of the PNA-DNA duplex stability. However, PNA possesses poor aqueous solubility and cannot directly penetrate cell membranes. These are major obstacles that limit in vivo applications of PNA. In previous strategies, the PNA can be conjugated to macromolecular carriers or modified with positively charged side chains such as guanidinium groups to improve the aqueous solubility and cell permeability. In general, a preformed modified PNA monomer was required. In this study, a new approach for post-synthetic modification of PNA backbone with one or more hydrophilic groups was proposed. The PNA used in this study was the conformationally constrained pyrrolidinyl PNA with prolyl-2-aminocyclopentanecarboxylic acid dipeptide backbone (acpcPNA) that shows several advantages over the conventional PNA. The aldehyde modifiers carrying different linkers (alkylene and oligo(ethylene glycol)) and end groups (-OH, -NH , and guanidinium) were synthesized and attached to the backbone of modified acpcPNA by reductive alkylation. The hybrids between the modified acpcPNAs and DNA exhibited comparable or superior thermal stability with base-pairing specificity similar to those of unmodified acpcPNA. Moreover, the modified apcPNAs also showed the improvement of aqueous solubility (10-20 folds compared to unmodified PNA) and readily penetrate cell membranes without requiring any special delivery agents. This study not only demonstrates the practicality of the proposed post-synthetic modification approach for PNA modification, which could be readily applied to other systems, but also opens up opportunities for using pyrrolidinyl PNA in various applications such as intracellular RNA sensing, specific gene detection, and antisense and antigene therapy.
[Mh] Termos MeSH primário: Cicloleucina/análogos & derivados
Dipeptídeos/química
Ácidos Nucleicos Peptídicos/química
Pirrolidinas/química
[Mh] Termos MeSH secundário: Permeabilidade da Membrana Celular
Cicloleucina/síntese química
Cicloleucina/metabolismo
Dipeptídeos/síntese química
Dipeptídeos/metabolismo
Células HEK293
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Ácidos Nucleicos Peptídicos/síntese química
Ácidos Nucleicos Peptídicos/metabolismo
Permeabilidade
Pirrolidinas/síntese química
Pirrolidinas/metabolismo
Solubilidade
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dipeptides); 0 (Peptide Nucleic Acids); 0 (Pyrrolidines); 0TQU7668EI (Cycloleucine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00308


  6 / 2038 MEDLINE  
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[PMID]:28696677
[Au] Autor:Nakano K; Tanabe J; Ishimatsu R; Imato T
[Ad] Endereço:Department of Applied Chemistry, Faculty of Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka, Japan.
[Ti] Título:Monolithic Peptide-Nucleic Acid Hybrid Functioning as an Artificial Microperoxidase.
[So] Source:Bioconjug Chem;28(8):2031-2034, 2017 Aug 16.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new peptide nucleic acid (PNA) with an installed peroxidase function has been developed. Fmoc solid phase peptide synthesis prepared a PNA hybrid (VQKCAQCHTVE-(C H O) CH -[PNA(T)] -G) that renders the microperoxidase backbone, followed by reconstitution with hemin. The resulting holocompound catalyzed the oxidation of 3,3',5,5'-tetramthylbenzidine by H O to 50% that of natural microperoxidase-11, whereas the apo-form and hemin gave no responses. The peroxidase domain was found to be active toward direct electrochemistry and the PNA hybrid served for gene sensor; in the presence of the target DNA (5'-CATGTATAAAAAA-3'), an electrode-attached DNA probe (5'-TsTsTsTsTsTCTCATACATG-3') showed the ferric-to-ferrous quasi-reversible wave (-276 mV vs Ag/AgCl) through sandwich hybridization. Moreover, the hybridization product could accept H O as an oxidant to enhance the reduction current, which occurred likely based on the iron(II)-center-recycling with specific rate constant of 0.19 s .
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Materiais Biomiméticos/metabolismo
Ácidos Nucleicos Peptídicos/química
Ácidos Nucleicos Peptídicos/metabolismo
Peroxidases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Modelos Moleculares
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Nucleic Acids); EC 1.11.1.- (Peroxidases); EC 1.11.1.- (microperoxidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00216


  7 / 2038 MEDLINE  
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[PMID]:28624242
[Au] Autor:Saarbach J; Masi D; Zambaldo C; Winssinger N
[Ad] Endereço:Faculty of Science, Department of Organic Chemistry, NCCR Chemical Biology, University of Geneva, 30 quai Ernest Ansermet, Geneva, Switzerland.
[Ti] Título:Facile access to modified and functionalized PNAs through Ugi-based solid phase oligomerization.
[So] Source:Bioorg Med Chem;25(19):5171-5177, 2017 Oct 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peptide nucleic acids (PNAs) derivatized with functional molecules are increasingly used in diverse biosupramolecular applications. PNAs have proven to be highly tolerant to modifications and different applications benefit from the use of modified PNAs, in particular modifications at the γ position. Herein we report simple protocols to access modified PNAs from iterative Ugi couplings which allow modular modifications at the α, ß or γ position of the PNA backbone from simple starting materials. We demonstrate the utility of the method with the synthesis of several bioactive small molecules (a peptide ligand, a kinase inhibitor and a glycan)-PNA conjugates.
[Mh] Termos MeSH primário: Ácidos Nucleicos Peptídicos/síntese química
Bibliotecas de Moléculas Pequenas/síntese química
Técnicas de Síntese em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Glicoconjugados/síntese química
Glicoconjugados/química
Ligantes
Ácidos Nucleicos Peptídicos/química
Peptídeos/síntese química
Peptídeos/química
Inibidores de Proteínas Quinases/síntese química
Inibidores de Proteínas Quinases/química
Bibliotecas de Moléculas Pequenas/química
Técnicas de Síntese em Fase Sólida/economia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoconjugates); 0 (Ligands); 0 (Peptide Nucleic Acids); 0 (Peptides); 0 (Protein Kinase Inhibitors); 0 (Small Molecule Libraries)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170619
[St] Status:MEDLINE


  8 / 2038 MEDLINE  
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[PMID]:28617037
[Au] Autor:Tian Q; Rogness J; Meng M; Li Z
[Ad] Endereço:Bioanalysis, Arrowhead Pharmaceuticals, Inc., 502 S. Rosa Road, Madison, WI 53719, USA.
[Ti] Título:Quantitative determination of a siRNA (AD00370) in rat plasma using peptide nucleic acid probe and HPLC with fluorescence detection.
[So] Source:Bioanalysis;9(11):861-872, 2017 Jun.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: Toxicokinetic and pharmacokinetic studies of therapeutic oligonucleotides require validated bioanalytical methods for sensitive and specific quantification of oligonucleotide drug candidates in biological samples. RESULTS: A peptide nucleic acid (PNA) hybridization-based HPLC-fluorescence assay was developed and validated for quantification of Arrowhead Pharmaceuticals' proprietary siRNA in rat plasma samples via hybridization and anion-exchange-HPLC (AEX-HPLC) with fluorescence detection. CONCLUSION: The validated method provided a sensitive and selective approach for quantification of siRNA in biological samples at a linear quantitation range of 1-1000 ng/ml. The assay requires only 25 µl of plasma sample and shows excellent accuracy and precision even without using an internal standard, providing a useful quantification method for siRNA determination in biological matrix with limited sample volume.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Hibridização de Ácido Nucleico/métodos
Ácidos Nucleicos Peptídicos/química
RNA Interferente Pequeno/análise
RNA Interferente Pequeno/sangue
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Fluorescência
Limite de Detecção
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Peptide Nucleic Acids); 0 (RNA, Small Interfering)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.4155/bio-2017-0017


  9 / 2038 MEDLINE  
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[PMID]:28614621
[Au] Autor:Taskova M; Mantsiou A; Astakhova K
[Ad] Endereço:Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark.
[Ti] Título:Synthetic Nucleic Acid Analogues in Gene Therapy: An Update for Peptide-Oligonucleotide Conjugates.
[So] Source:Chembiochem;18(17):1671-1682, 2017 Sep 05.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The main objective of this work is to provide an update on synthetic nucleic acid analogues and nanoassemblies as tools in gene therapy. In particular, the synthesis and properties of peptide-oligonucleotide conjugates (POCs), which have high potential in research and as therapeutics, are described in detail. The exploration of POCs has already led to fruitful results in the treatment of neurological diseases, lung disorders, cancer, leukemia, viral, and bacterial infections. However, delivery and in vivo stability are the major barriers to the clinical application of POCs and other analogues that still have to be overcome. This review summarizes recent achievements in the delivery and in vivo administration of synthetic nucleic acid analogues, focusing on POCs, and compares their efficiency.
[Mh] Termos MeSH primário: Ácidos Nucleicos/química
Oligonucleotídeos/química
Peptídeos/química
[Mh] Termos MeSH secundário: Terapia Genética
Seres Humanos
Neoplasias/terapia
Ácidos Nucleicos/síntese química
Ácidos Nucleicos/metabolismo
Ácidos Nucleicos Peptídicos/química
Interferência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nucleic Acids); 0 (Oligonucleotides); 0 (Peptide Nucleic Acids); 0 (Peptides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700229


  10 / 2038 MEDLINE  
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[PMID]:28610975
[Au] Autor:Sugiyama T; Hasegawa G; Niikura C; Kuwata K; Imamura Y; Demizu Y; Kurihara M; Kittaka A
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Teikyo University, Itabashi-ku, Tokyo 173-8605, Japan. Electronic address: tsugiyama@pharm.teikyo-u.ac.jp.
[Ti] Título:PNA monomers fully compatible with standard Fmoc-based solid-phase synthesis of pseudocomplementary PNA.
[So] Source:Bioorg Med Chem Lett;27(15):3337-3341, 2017 08 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Here we report the synthesis of new PNA monomers for pseudocomplementary PNA (pcPNA) that are fully compatible with standard Fmoc chemistry. The thiocarbonyl group of the 2-thiouracil (sU) monomer was protected with the 4-methoxy-2-methybenzyl group (MMPM), while the exocyclic amino groups of diaminopurine (D) were protected with Boc groups. The newly synthesized monomers were incorporated into a 10-mer PNA oligomer using standard Fmoc chemistry for solid-phase synthesis. Oligomerization proceeded smoothly and the HPLC and MALDI-TOF MS analyses indicated that there was no remaining MMPM on the sU nucleobase. The new PNA monomers reported here would facilitate a wide range of applications, such as antigene PNAs and DNA nanotechnologies.
[Mh] Termos MeSH primário: Ácidos Nucleicos Peptídicos/síntese química
Técnicas de Síntese em Fase Sólida
[Mh] Termos MeSH secundário: Estrutura Molecular
Ácidos Nucleicos Peptídicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Peptide Nucleic Acids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE



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