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[PMID]:27135402
[Au] Autor:Seow N; Kirk Y; Yung LY
[Ad] Endereço:Department of Chemical and Biomolecular Engineering, Faculty of Engineering, National University of Singapore , Singapore 119260, Singapore.
[Ti] Título:Detection of G-Quadruplex Formation via Light Scattering of Defined Gold Nanoassemblies Modulated by Molecular Hairpins.
[So] Source:Bioconjug Chem;27(5):1236-43, 2016 May 18.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:G-quadruplexes are of great scientific interest, as these unique DNA structures play key regulatory roles in cell replication, such as safeguarding against uncontrolled cellular divisions. The quadruplexes have also been applied for detecting DNA and protein biomarkers via methods like fluorescence resonance energy transfer (FRET) and gold nanoparticle (AuNP) aggregation. As an alternative and complementary platform to the established molecular techniques for the study of quadruplexes, we have developed a strategy coupling poly-G (PG)-mediated quadruplex formation with AuNP assembly detectable via dynamic light scattering (DLS). The presence of quadruplex-forming sequences also uniquely modifies the AuNP nanoassembly readout on DLS. In addition, molecular hairpins co-attached onto the AuNP together with PG successfully modulated the quadruplex-induced nanoassembly. Through molecular beacon-based fluorescence restoration and light scattering signal changes, the open/closed conformations of the hairpins are leveraged to tune the size of the quadruplex-mediated nanoassembly.
[Mh] Termos MeSH primário: DNA/química
Difusão Dinâmica da Luz
Quadruplex G
Ouro/química
Sequências Repetidas Invertidas
Nanopartículas Metálicas/química
[Mh] Termos MeSH secundário: DNA/genética
Transferência Ressonante de Energia de Fluorescência
Sondas de Oligonucleotídeos/química
Tamanho da Partícula
Poli G/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotide Probes); 25191-14-4 (Poly G); 7440-57-5 (Gold); 9007-49-2 (DNA)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.6b00084


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[PMID]:26742581
[Au] Autor:Raveendran D; Raghavan SC
[Ad] Endereço:Department of Biochemistry, Indian Institute of Science, Bangalore, 560 012, India.
[Ti] Título:Biochemical Characterization of Nonamer Binding Domain of RAG1 Reveals its Thymine Preference with Respect to Length and Position.
[So] Source:Sci Rep;6:19091, 2016 Jan 08.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:RAG complex consisting of RAG1 and RAG2 is a site-specific endonuclease responsible for the generation of antigen receptor diversity. It cleaves recombination signal sequence (RSS), comprising of conserved heptamer and nonamer. Nonamer binding domain (NBD) of RAG1 plays a central role in the recognition of RSS. To investigate the DNA binding properties of the domain, NBD of murine RAG1 was cloned, expressed and purified. Electrophoretic mobility shift assays showed that NBD binds with high affinity to nonamer in the context of 12/23 RSS or heteroduplex DNA. NBD binding was specific to thymines when single stranded DNA containing poly A, C, G or T were used. Biolayer interferometry studies showed that poly T binding to NBD was robust and comparable to that of 12RSS. More than 23 nt was essential for NBD binding at homothymidine stretches. On a double-stranded DNA, NBD could bind to A:T stretches, but not G:C or random sequences. Although NBD is indispensable for sequence specific activity of RAGs, external supplementation of purified nonamer binding domain to NBD deleted cRAG1/cRAG2 did not restore its activity, suggesting that the overall domain architecture of RAG1 is important. Therefore, we define the sequence requirements of NBD binding to DNA.
[Mh] Termos MeSH primário: DNA de Cadeia Simples/química
Proteínas de Ligação a DNA/química
Proteínas de Homeodomínio/química
Poli T/química
Receptores de Antígenos/química
[Mh] Termos MeSH secundário: Animais
Linfócitos B/citologia
Linfócitos B/imunologia
Linfócitos B/metabolismo
Sequência de Bases
Sítios de Ligação
Clonagem Molecular
DNA de Cadeia Simples/genética
Proteínas de Ligação a DNA/genética
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Proteínas de Homeodomínio/genética
Camundongos
Poli A/química
Poli C/química
Poli G/química
Ligação Proteica
Domínios Proteicos
Sinais Direcionadores de Proteínas
Receptores de Antígenos/genética
Receptores de Antígenos/imunologia
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Linfócitos T/citologia
Linfócitos T/imunologia
Linfócitos T/metabolismo
Recombinação V(D)J
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Single-Stranded); 0 (DNA-Binding Proteins); 0 (Homeodomain Proteins); 0 (Protein Sorting Signals); 0 (Rag2 protein, mouse); 0 (Receptors, Antigen); 0 (Recombinant Proteins); 128559-51-3 (RAG-1 protein); 24937-83-5 (Poly A); 25086-81-1 (Poly T); 25191-14-4 (Poly G); 30811-80-4 (Poly C)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.1038/srep19091


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[PMID]:26232404
[Au] Autor:Li S; Wang L; Han F; Gong Q; Yu W
[Ad] Endereço:Key Laboratory of Marine Drugs, Chinese Ministry of Education; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology; School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
[Ti] Título:Cloning and characterization of the first polysaccharide lyase family 6 oligoalginate lyase from marine Shewanella sp. Kz7.
[So] Source:J Biochem;159(1):77-86, 2016 Jan.
[Is] ISSN:1756-2651
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Alginate, the most abundant carbohydrate in brown macroalgae, is widely used in the food and pharmaceutical industries. Recently, alginate has attracted increasing attention, as it may serve as an alternative biomass for the production of biofuel. The degradation of alginate into monomeric units is the prerequisite for bioethanol production. All known oligoalginate lyases belong to the polysaccharide lyase (PL) family 7, 14, 15 and 17, and most of them preferred to degrade the polyM blocks to yield 4-deoxy-l-erythro-5-hexoseulose uronic acid as the primary product. In this study, we cloned an oligoalginate lyase gene, oalS6, from Shewanella sp. Kz7 and expressed it in Escherichia coli. The PL family 6 oligoalginate lyase (OalS6) has no significant sequence similarity with other known oligoalginate lyases. OalS6 contains a chondroitinase-like domain and was assigned to the PL family 6. This lyase is an exo-type oligoalginate lyase and prefer to depolymerize polyG block into 2, 4, 5, 6-tetrahydroxytetrahydro-2H-pyran-2-carboxylic acid. All of these results indicate that OalS6 is a novel oligoalginate lyase that is structurally and functionally different from other known oligoalginate lyases. This finding provides new insights into the development of biofuel processing biotechnologies from seaweed.
[Mh] Termos MeSH primário: Alginatos/metabolismo
Proteínas de Bactérias/química
Biocombustíveis
Polissacarídeo-Liase/química
Alga Marinha/química
Shewanella/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/genética
Biotecnologia
Clonagem Molecular
Eletroforese em Gel de Poliacrilamida
Ácido Glucurônico/metabolismo
Ácidos Hexurônicos/metabolismo
Concentração de Íons de Hidrogênio
Cinética
Dados de Sequência Molecular
Poli G/metabolismo
Polissacarídeo-Liase/genética
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alginates); 0 (Bacterial Proteins); 0 (Biofuels); 0 (Hexuronic Acids); 25191-14-4 (Poly G); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); EC 4.2.2.- (Polysaccharide-Lyases); EC 4.2.2.3 (poly(beta-D-mannuronate) lyase)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150802
[St] Status:MEDLINE
[do] DOI:10.1093/jb/mvv076


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[PMID]:26612536
[Au] Autor:Zhao XL; Chen BC; Han JC; Wei L; Pan XB
[Ad] Endereço:Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases; Beijing 100044, P.R. China.
[Ti] Título:Delivery of cell-penetrating peptide-peptide nucleic acid conjugates by assembly on an oligonucleotide scaffold.
[So] Source:Sci Rep;5:17640, 2015 Nov 27.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Delivery to intracellular target sites is still one of the main obstacles in the development of peptide nucleic acids (PNAs) as antisense-antigene therapeutics. Here, we designed a self-assembled oligonucleotide scaffold that included a central complementary region for self-assembly and lateral regions complementing the PNAs. Assembly of cell-penetrating peptide (CPP)-PNAs on the scaffold significantly promoted endocytosis of PNAs by at least 10-fold in cell cultures, particularly for scaffolds in which the central complementary region was assembled by poly(guanine) and poly(cytosine). The antisense activity of CPP-PNAs increased by assembly on the scaffold and was further enhanced after co-assembly with endosomolytic peptide (EP)-PNA. This synergistic effect was also observed following the assembly of antigene CPP-PNAs\EP-PNAs on the scaffold. However, antigene activity was only observed by targeting episomal viral DNA or transfected plasmids, but not the chromosome in the cell cultures. In conclusion, assembly on oligonucleotide scaffolds significantly enhanced the antisense-antigene activity of PNAs by promoting endocytosis and endosomal escape. This oligonucleotide scaffold provided a simple strategy for assembly of multiple functional peptide-PNA conjugates, expanding the applications of PNAs and demonstrating the potential of PNAs as antiviral therapeutics.
[Mh] Termos MeSH primário: Antivirais/metabolismo
Peptídeos Penetradores de Células/genética
Técnicas de Transferência de Genes
Oligonucleotídeos Antissenso/genética
Ácidos Nucleicos Peptídicos/genética
[Mh] Termos MeSH secundário: Antivirais/química
Sequência de Bases
Peptídeos Penetradores de Células/síntese química
Peptídeos Penetradores de Células/metabolismo
DNA Viral/genética
DNA Viral/metabolismo
Endocitose
Endossomos/metabolismo
Células HeLa
Células Hep G2
Vírus da Hepatite B/genética
Vírus da Hepatite B/metabolismo
Seres Humanos
Dados de Sequência Molecular
Oligonucleotídeos Antissenso/síntese química
Oligonucleotídeos Antissenso/metabolismo
Ácidos Nucleicos Peptídicos/síntese química
Ácidos Nucleicos Peptídicos/metabolismo
Plasmídeos/química
Plasmídeos/metabolismo
Poli C/química
Poli C/metabolismo
Poli G/química
Poli G/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Cell-Penetrating Peptides); 0 (DNA, Viral); 0 (Oligonucleotides, Antisense); 0 (Peptide Nucleic Acids); 25191-14-4 (Poly G); 30811-80-4 (Poly C)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151128
[St] Status:MEDLINE
[do] DOI:10.1038/srep17640


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[PMID]:26538384
[Au] Autor:Eidelshtein G; Kotlyar A; Hashemi M; Gurevich L
[Ad] Endereço:Department of Biochemistry and Molecular Biology, George S Wise Faculty of Life Sciences and The Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.
[Ti] Título:Aligned deposition and electrical measurements on single DNA molecules.
[So] Source:Nanotechnology;26(47):475102, 2015 Nov 27.
[Is] ISSN:1361-6528
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A reliable method of deposition of aligned individual dsDNA molecules on mica, silicon, and micro/nanofabricated circuits is presented. Complexes of biotinylated double stranded poly(dG)-poly(dC) DNA with avidin were prepared and deposited on mica and silicon surfaces in the absence of Mg(2+) ions. Due to its positive charge, the avidin attached to one end of the DNA anchors the complex to negatively charged substrates. Subsequent drying with a directional gas flow yields DNA molecules perfectly aligned on the surface. In the avidin-DNA complex only the avidin moiety is strongly and irreversibly bound to the surface, while the DNA counterpart interacts with the substrates much more weakly and can be lifted from the surface and realigned in any direction. Using this technique, avidin-DNA complexes were deposited across platinum electrodes on a silicon substrate. Electrical measurements on the deposited DNA molecules revealed linear IV-characteristics and exponential dependence on relative humidity.
[Mh] Termos MeSH primário: DNA/química
Nanotecnologia/métodos
[Mh] Termos MeSH secundário: Silicatos de Alumínio/química
Avidina/química
Fenômenos Eletromagnéticos
Umidade
Microeletrodos
Microscopia de Força Atômica
Poli C/química
Poli G/química
Silício/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aluminum Silicates); 1405-69-2 (Avidin); 25191-14-4 (Poly G); 25609-92-1 (poly(dC)); 25656-92-2 (poly(dG)); 30811-80-4 (Poly C); 9007-49-2 (DNA); V8A1AW0880 (mica); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151114
[Lr] Data última revisão:
151114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151106
[St] Status:MEDLINE
[do] DOI:10.1088/0957-4484/26/47/475102


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[PMID]:26449960
[Au] Autor:Ryazanova O; Zozulya V; Voloshin I; Dubey L; Dubey I; Karachevtsev V
[Ad] Endereço:Department of Molecular Biophysics, B. Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, 47 Lenin ave, 61103, Kharkov, Ukraine. ryazanova@ilt.kharkov.ua.
[Ti] Título:Binding of Metallated Porphyrin-Imidazophenazine Conjugate to Tetramolecular Quadruplex Formed by Poly(G): a Spectroscopic Investigation.
[So] Source:J Fluoresc;25(6):1897-904, 2015 Nov.
[Is] ISSN:1573-4994
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The binding of telomerase inhibitor ZnTMPyP(3+)-ImPzn, Zn(II) derivative of tricationic porphyrin-imidazophenazine conjugate, to tetramolecular quadruplex structure formed by poly(G) was studied in aqueous solutions at neutral pH and near physiological ionic strength using absorption and polarized fluorescent spectroscopy techniques. Three binding modes were determined from the dependences of the fluorescence intensity and polarization degree for the porphyrin and phenazine moieties of the conjugate on molar polymer-to-dye ratio (P/D). The first one is outside electrostatic binding of positively charged porphyrin fragments to anionic phosphate groups of the polymer which prevails only at very low P/D values and manifests itself by substantial fluorescence quenching. It is suggested that the formation of externally bound porphyrin dimers occurs. The other two binding modes observed at high P/D are embedding of the ZnTMPyP(3+) moiety into the groove of poly(G) quadruplex accompanied by more than 3-fold enhancement of the conjugate emission, and simultaneous intercalation of the phenazine fragment between the guanine bases accompanied by the increase of its fluorescence polarization degree up to 0.25. Thus Zn(II) conjugate seems to be promising ligand for the stabilization of G-quadruplex structures since porphyrin binding to poly(G) is strengthened by additional intercalation of phenazine moiety.
[Mh] Termos MeSH primário: Quadruplex G
Metaloporfirinas/química
Fenazinas/química
Poli G/química
Zinco/química
[Mh] Termos MeSH secundário: Sequência de Bases
Poli G/genética
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metalloporphyrins); 0 (Phenazines); 25191-14-4 (Poly G); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151010
[St] Status:MEDLINE
[do] DOI:10.1007/s10895-015-1682-2


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[PMID]:26227667
[Au] Autor:Fattal I; Shental N; Ben-Dor S; Molad Y; Gabrielli A; Pokroy-Shapira E; Oren S; Livneh A; Langevitz P; Zandman-Goddard G; Sarig O; Margalit R; Gafter U; Domany E; Cohen IR
[Ad] Endereço:Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
[Ti] Título:Guanine polynucleotides are self-antigens for human natural autoantibodies and are significantly reduced in the human genome.
[So] Source:Immunology;146(3):401-10, 2015 Nov.
[Is] ISSN:1365-2567
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the course of investigating anti-DNA autoantibodies, we examined IgM and IgG antibodies to poly-G and other oligonucleotides in the sera of healthy persons and those diagnosed with systemic lupus erythematosus (SLE), scleroderma (SSc), or pemphigus vulgaris (PV); we used an antigen microarray and informatic analysis. We now report that all of the 135 humans studied, irrespective of health or autoimmune disease, manifested relatively high amounts of IgG antibodies binding to the 20-mer G oligonucleotide (G20); no participants entirely lacked this reactivity. IgG antibodies to homo-nucleotides A20, C20 or T20 were present only in the sera of SLE patients who were positive for antibodies to dsDNA. The prevalence of anti-G20 antibodies led us to survey human, mouse and Drosophila melanogaster (fruit fly) genomes for runs of T20 and G20 or more: runs of T20 appear > 170,000 times compared with only 93 runs of G20 or more in the human genome; of these runs, 40 were close to brain-associated genes. Mouse and fruit fly genomes showed significantly lower T20/G20 ratios than did human genomes. Moreover, sera from both healthy and SLE mice contained relatively little or no anti-G20 antibodies; so natural anti-G20 antibodies appear to be characteristic of humans. These unexpected observations invite investigation of the immune functions of anti-G20 antibodies in human health and disease and of runs of G20 in the human genome.
[Mh] Termos MeSH primário: Autoanticorpos/sangue
Autoantígenos/genética
Autoantígenos/imunologia
Poli G/genética
Poli G/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antinucleares/sangue
Estudos de Casos e Controles
Ilhas de CpG
Drosophila melanogaster/genética
Feminino
Genoma Humano
Genoma de Inseto
Seres Humanos
Imunidade Inata
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Lúpus Eritematoso Sistêmico/genética
Lúpus Eritematoso Sistêmico/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos NZB
Pênfigo/genética
Pênfigo/imunologia
Poli T/genética
Poli T/imunologia
Escleroderma Sistêmico/genética
Escleroderma Sistêmico/imunologia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Antinuclear); 0 (Autoantibodies); 0 (Autoantigens); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 25086-81-1 (Poly T); 25191-14-4 (Poly G)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161101
[Lr] Data última revisão:
161101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150801
[St] Status:MEDLINE
[do] DOI:10.1111/imm.12514


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[PMID]:26076929
[Au] Autor:Ryazanova O; Zozulya V; Voloshin I; Dubey L; Dubey I; Karachevtsev V
[Ad] Endereço:Department of Molecular Biophysics, B. Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, 47 Lenin ave, 61103, Kharkov, Ukraine, ryazanova@ilt.kharkov.ua.
[Ti] Título:Spectroscopic Studies on Binding of Porphyrin-Phenazine Conjugate to Four-Stranded Poly(G).
[So] Source:J Fluoresc;25(4):1013-21, 2015 Jul.
[Is] ISSN:1573-4994
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Binding of a novel cationic porphyrin-imidazophenazine conjugate, TMPyP(3+)-ImPzn, to four-stranded poly(G) was investigated in aqueous solutions of neutral pH under near physiological ionic conditions using absorption, polarized fluorescent spectroscopy and fluorescence titration techniques. In absence of the polymer the conjugate folds into stable internal heterodimer with stacking between the porphyrin and phenazine chromophores. Binding of TMPyP(3+)-ImPzn to poly(G) is realized by two competing ways. At low polymer-to-dye ratio (P/D < 6) outside electrostatic binding of the cationic porphyrin moieties of the conjugate to anionic polynucleotide backbone with their self-stacking is predominant. It is accompanied by heterodimer dissociation and distancing of phenazine moieties from the polymer. This binding mode is characterized by strong quenching of the conjugate fluorescence. Increase of P/D results in the disintegration of the porphyrin stacks and redistribution of the bound conjugate molecules along the polymer chain. At P/D > 10 another binding mode becomes dominant, embedding of TMPyP(3+)-ImPzn heterodimers into poly(G) groove as a whole is occurred.
[Mh] Termos MeSH primário: Morfolinas/química
Fenazinas/química
Poli G/química
Polímeros/química
Porfirinas/química
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: Sítios de Ligação
Dicroísmo Circular
Fluorescência
Morfolinas/metabolismo
Fenazinas/metabolismo
Poli G/metabolismo
Polímeros/metabolismo
Porfirinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Morpholines); 0 (Phenazines); 0 (Polymers); 0 (Porphyrins); 25191-14-4 (Poly G); 38673-65-3 (tetra(4-N-methylpyridyl)porphine); AB2794W8KV (phendimetrazine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150617
[St] Status:MEDLINE
[do] DOI:10.1007/s10895-015-1585-2


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[PMID]:26038313
[Au] Autor:Hase K; Fujiwara Y; Kikuchi H; Aizawa S; Hakuno F; Takahashi S; Wada K; Kabuta T
[Ad] Endereço:Department of Degenerative Neurological Disease, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan Department of Animal Sciences and Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The U
[Ti] Título:RNautophagy/DNautophagy possesses selectivity for RNA/DNA substrates.
[So] Source:Nucleic Acids Res;43(13):6439-49, 2015 Jul 27.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lysosomes can degrade various biological macromolecules, including nucleic acids, proteins and lipids. Recently, we identified novel nucleic acid-degradation systems termed RNautophagy/DNautophagy (abbreviated as RDA), in which RNA and DNA are directly taken up by lysosomes in an ATP-dependent manner and degraded. We also found that a lysosomal membrane protein, LAMP2C, the cytoplasmic region of which binds to RNA and DNA, functions, at least in part, as an RNA/DNA receptor in the process of RDA. However, it has been unclear whether RDA possesses selectivity for RNA/DNA substrates and the RNA/DNA sequences that are recognized by LAMP2C have not been determined. In the present study, we found that the cytosolic region of LAMP2C binds to poly-G/dG, but not to poly-A/dA, poly-C/dC, poly-dT or poly-U. Consistent with this binding activity, poly-G/dG was transported into isolated lysosomes via RDA, while poly-A/dA, poly-C/dC, poly-dT and poly-U were not. GGGGGG or d(GGGG) sequences are essential for the interaction between poly-G/dG and LAMP2C. In addition to poly-G/dG, G/dG-rich sequences, such as a repeated GGGGCC sequence, interacted with the cytosolic region of LAMP2C. Our findings indicate that RDA does possess selectivity for RNA/DNA substrates and that at least some consecutive G/dG sequence(s) can mediate RDA.
[Mh] Termos MeSH primário: Autofagia
DNA/metabolismo
Proteína 2 de Membrana Associada ao Lisossomo/metabolismo
RNA/metabolismo
[Mh] Termos MeSH secundário: Animais
DNA/química
Metilação de DNA
Lisossomos/metabolismo
Camundongos
Poli G/metabolismo
RNA/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lysosomal-Associated Membrane Protein 2); 25191-14-4 (Poly G); 25656-92-2 (poly(dG)); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151111
[Lr] Data última revisão:
151111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150604
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkv579


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[PMID]:25479163
[Au] Autor:Murray V
[Ad] Endereço:School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: v.murray@unsw.edu.au.
[Ti] Título:The frequency of poly(G) tracts in the human genome and their use as a sensor of DNA damage.
[So] Source:Comput Biol Chem;54:13-7, 2015 Feb.
[Is] ISSN:1476-928X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tandem repeats of short DNA sequences are commonly found in human DNA. These simple sequence repeats or microsatellites are highly polymorphic in the human genome. Since the anti-tumour agent cisplatin preferentially forms DNA adducts at runs of consecutive guanine nucleotides (poly(G)), the position and frequency of occurrence of poly(G) sequences in the updated human genome was investigated. There are more runs of consecutive guanines than would be expected by random chance. This especially true for poly(G) sequences longer than approximately n=9. A plot of poly(G) length against log(observed/expected) frequency produced a straight line for n>9. A similar observation was also found for poly(A) DNA sequence repeats. This data implied that the increase in observed/expected frequency is directly related to length of DNA repeat. It was proposed that long runs of consecutive guanine nucleotides could be a sensitive sensor of cellular DNA damage since a number of DNA damaging agents cause lesions at poly(G) sequences.
[Mh] Termos MeSH primário: DNA/química
Genoma Humano
Sondas Moleculares/química
Poli A/química
Poli G/química
[Mh] Termos MeSH secundário: Sequência de Bases
DNA/genética
Dano ao DNA
Seres Humanos
Repetições de Microssatélites
Sondas Moleculares/genética
Dados de Sequência Molecular
Poli A/genética
Poli G/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Molecular Probes); 24937-83-5 (Poly A); 25191-14-4 (Poly G); 9007-49-2 (DNA)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150209
[Lr] Data última revisão:
150209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141206
[St] Status:MEDLINE



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