[PMID]: | 25057007 |
[Au] Autor: | Epaulard O; Adam L; Poux C; Zurawski G; Salabert N; Rosenbaum P; Dereuddre-Bosquet N; Zurawski S; Flamar AL; Oh S; Romain G; Chapon C; Banchereau J; Lévy Y; Le Grand R; Martinon F |
[Ad] Endereço: | French Alternative Energies and Atomic Energy Commission, Division of Immuno-Virology, Institute for Emerging Diseases and Innovative Therapies, Infectious Diseases Models for Innovative Therapies Center, 92265 Fontenay-aux-Roses, France; Unité Mixte de Recherche E1, Université Paris-Sud, 91405 Orsa |
[Ti] Título: | Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses. |
[So] Source: | J Immunol;193(5):2416-26, 2014 Sep 01. |
[Is] ISSN: | 1550-6606 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Dendritic cells are major APCs that can efficiently prime immune responses. However, the roles of skin-resident Langerhans cells (LCs) in eliciting immune responses have not been fully understood. In this study, we demonstrate for the first time, to our knowledge, that LCs in cynomolgus macaque skin are capable of inducing antiviral-specific immune responses in vivo. Targeting HIV-Gag or influenza hemagglutinin Ags to skin LCs using recombinant fusion proteins of anti-Langerin Ab and Ags resulted in the induction of the viral Ag-specific responses. We further demonstrated that such Ag-specific immune responses elicited by skin LCs were greatly enhanced by TLR ligands, polyriboinosinic polyribocytidylic acid, and R848. These enhancements were not due to the direct actions of TLR ligands on LCs, but mainly dependent on TNF-α secreted from macrophages and neutrophils recruited to local tissues. Skin LC activation and migration out of the epidermis are associated with macrophage and neutrophil infiltration into the tissues. More importantly, blocking TNF-α abrogated the activation and migration of skin LCs. This study highlights that the cross-talk between innate immune cells in local tissues is an important component for the establishment of adaptive immunity. Understanding the importance of local immune networks will help us to design new and effective vaccines against microbial pathogens. |
[Mh] Termos MeSH primário: |
HIV-1/imunologia Glicoproteínas de Hemaglutininação de Vírus da Influenza/farmacologia Vírus da Influenza A/imunologia Células de Langerhans/imunologia Pele/imunologia Fator de Necrose Tumoral alfa/imunologia Produtos do Gene gag do Vírus da Imunodeficiência Humana/farmacologia
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[Mh] Termos MeSH secundário: |
Imunidade Adaptativa/efeitos dos fármacos Imunidade Adaptativa/fisiologia Animais Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia Imidazóis/farmacologia Macaca mulatta Macrófagos/imunologia Neutrófilos/imunologia Poli I/farmacologia Produtos do Gene gag do Vírus da Imunodeficiência Humana/imunologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (Imidazoles); 0 (Tumor Necrosis Factor-alpha); 0 (gag Gene Products, Human Immunodeficiency Virus); 25249-22-3 (Poly I); V3DMU7PVXF (resiquimod) |
[Em] Mês de entrada: | 1412 |
[Cu] Atualização por classe: | 161019 |
[Lr] Data última revisão:
| 161019 |
[Sb] Subgrupo de revista: | AIM; IM |
[Da] Data de entrada para processamento: | 140725 |
[St] Status: | MEDLINE |
[do] DOI: | 10.4049/jimmunol.1303339 |
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