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[PMID]: 28696256 [Au] Autor: Han S; Zhuang H; Shumyak S; Wu J; Li H; Yang LJ; Reeves WH [Ad] Endereço: Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Florida, Gainesville, FL 32610; and. [Ti] Título: A Novel Subset of Anti-Inflammatory CD138 Macrophages Is Deficient in Mice with Experimental Lupus. [So] Source: J Immunol;199(4):1261-1274, 2017 Aug 15. [Is] ISSN: 1550-6606 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Dead cells accumulating in the tissues may contribute to chronic inflammation. We examined the cause of impaired apoptotic cell clearance in human and murine lupus. Dead cells accumulated in bone marrow from lupus patients but not from nonautoimmune patients undergoing myeloablation, where they were efficiently removed by macrophages (MΦ). Impaired apoptotic cell uptake by MΦ also was seen in mice treated i.p. with pristane (develop lupus) but not mineral oil (MO) (do not develop lupus). The inflammatory response to both pristane and MO rapidly depleted resident (Tim4 ) large peritoneal MΦ. The peritoneal exudate of pristane-treated mice contained mainly Ly6C inflammatory monocytes; whereas in MO-treated mice, it consisted predominantly of a novel subset of highly phagocytic MΦ resembling small peritoneal MΦ (SPM) that expressed CD138 and the scavenger receptor Marco. Treatment with anti-Marco-neutralizing Abs and the class A scavenger receptor antagonist polyinosinic acid inhibited phagocytosis of apoptotic cells by CD138 MΦ. CD138 MΦ expressed IL-10R, CD206, and CCR2 but little TNF-α or CX3CR1. They also expressed high levels of activated CREB, a transcription factor implicated in generating alternatively activated MΦ. Similar cells were identified in the spleen and lung of MO-treated mice and also were induced by LPS. We conclude that highly phagocytic, CD138 SPM-like cells with an anti-inflammatory phenotype may promote the resolution of inflammation in lupus and infectious diseases. These SPM-like cells are not restricted to the peritoneum and may help clear apoptotic cells from tissues such as the lung, helping to prevent chronic inflammation. [Mh] Termos MeSH primário: Lúpus Eritematoso Sistêmico/imunologia Macrófagos Peritoneais/imunologia Fagocitose Sindecana-1/imunologia [Mh] Termos MeSH secundário: Animais Antígenos Ly/análise Apoptose Células da Medula Óssea/imunologia Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo Modelos Animais de Doenças Seres Humanos Inflamação/imunologia Subunidade alfa de Receptor de Interleucina-10/genética Subunidade alfa de Receptor de Interleucina-10/imunologia Pulmão/citologia Pulmão/imunologia Lúpus Eritematoso Sistêmico/induzido quimicamente Lúpus Eritematoso Sistêmico/fisiopatologia Macrófagos Peritoneais/efeitos dos fármacos Macrófagos Peritoneais/patologia Proteínas de Membrana/genética Proteínas de Membrana/metabolismo Camundongos Óleo Mineral/farmacologia Poli I/farmacologia Receptores Imunológicos/genética Receptores Imunológicos/metabolismo Baço/citologia Baço/imunologia Sindecana-1/genética Terpenos/farmacologia Fator de Necrose Tumoral alfa/genética Fator de Necrose Tumoral alfa/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Ly); 0 (Creb1 protein, mouse); 0 (Cyclic AMP Response Element-Binding Protein); 0 (Interleukin-10 Receptor alpha Subunit); 0 (Ly-6C antigen, mouse); 0 (Marco protein, mouse); 0 (Membrane Proteins); 0 (Receptors, Immunologic); 0 (Sdc1 protein, mouse); 0 (Syndecan-1); 0 (TIM-4 protein, mouse); 0 (Terpenes); 0 (Tumor Necrosis Factor-alpha); 25249-22-3 (Poly I); 26HZV48DT1 (pristane); 8020-83-5 (Mineral Oil) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171016 [Lr] Data última revisão: 171016 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170712 [St] Status: MEDLINE [do] DOI: 10.4049/jimmunol.1700099

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[PMID]: 28115843 [Au] Autor: Roviello GN; Vicidomini C; Costanzo V; Roviello V [Ad] Endereço: CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters. [Ti] Título: Nucleic acid binding and other biomedical properties of artificial oligolysines. [So] Source: Int J Nanomedicine;11:5897-5904, 2016. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: In the present study, we report the interaction of an artificial oligolysine (referred to as AOL) realized in our laboratory with targets of biomedical importance. These included polyinosinic acid (poly rI) and its complex with polycytidylic acid (poly I:C), RNAs with well-known interferon-inducing ability, and double-stranded (ds) DNA. The ability of the peptide to bind both single-stranded poly rI and ds poly I:C RNAs emerged from our circular dichroism (CD) and ultraviolet (UV) studies. In addition, we found that AOL forms complexes with dsDNA, as shown by spectroscopic binding assays and UV thermal denaturation experiments. These findings are encouraging for the possible use of AOL in biomedicine for nucleic acid targeting and oligonucleotide condensation, with the latter being a key step preceding their clinical application. Moreover, we tested the ability of AOL to bind to proteins, using serum albumin as a model protein. We demonstrated the oligolysine-protein binding by CD experiments which suggested that AOL, positively charged under physiological conditions, binds to the protein regions rich in anionic residues. Finally, the morphology characterization of the solid oligolysine, performed by scanning electron microscopy, showed different crystal forms including cubic-shaped crystals confirming the high purity of AOL. [Mh] Termos MeSH primário: Ácidos Nucleicos/metabolismo Peptídeos/química Peptídeos/metabolismo [Mh] Termos MeSH secundário: Dicroísmo Circular DNA/química DNA/metabolismo Lisina Ácidos Nucleicos/química Poli I/química Poli I/metabolismo Poli I-C Ligação Proteica Desnaturação Proteica RNA de Cadeia Dupla/química RNA de Cadeia Dupla/metabolismo Soroalbumina Bovina/química Soroalbumina Bovina/metabolismo Raios Ultravioleta [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Nucleic Acids); 0 (Peptides); 0 (RNA, Double-Stranded); 25249-22-3 (Poly I); 27432CM55Q (Serum Albumin, Bovine); 9007-49-2 (DNA); K3Z4F929H6 (Lysine); O84C90HH2L (Poly I-C) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170125 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S121247

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[PMID]: 25343355 [Au] Autor: Ito M; Hayashi K; Adachi E; Minamisawa T; Homma S; Koido S; Shiba K [Ad] Endereço: Department of Oncology, The Jikei University School of Medicine, Tokyo, Japan; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan. [Ti] Título: Combinatorial contextualization of peptidic epitopes for enhanced cellular immunity. [So] Source: PLoS One;9(10):e110425, 2014. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Invocation of cellular immunity by epitopic peptides remains largely dependent on empirically developed protocols, such as interfusion of aluminum salts or emulsification using terpenoids and surfactants. To explore novel vaccine formulation, epitopic peptide motifs were co-programmed with structural motifs to produce artificial antigens using our "motif-programming" approach. As a proof of concept, we used an ovalbumin (OVA) system and prepared an artificial protein library by combinatorially polymerizing MHC class I and II sequences from OVA along with a sequence that tends to form secondary structures. The purified endotoxin-free proteins were then examined for their ability to activate OVA-specific T-cell hybridoma cells after being processed within dendritic cells. One clone, F37A (containing three MHC I and two MHC II OVA epitopes), possessed a greater ability to evoke cellular immunity than the native OVA or the other artificial antigens. The sensitivity profiles of drugs that interfered with the F37A uptake differed from those of the other artificial proteins and OVA, suggesting that alteration of the cross-presentation pathway is responsible for the enhanced immunogenicity. Moreover, F37A, but not an epitopic peptide, invoked cellular immunity when injected together with monophosphoryl lipid A (MPL), and retarded tumor growth in mice. Thus, an artificially synthesized protein antigen induced cellular immunity in vivo in the absence of incomplete Freund's adjuvant or aluminum salts. The method described here could be potentially used for developing vaccines for such intractable ailments as AIDS, malaria and cancer, ailments in which cellular immunity likely play a crucial role in prevention and treatment. [Mh] Termos MeSH primário: Técnicas de Química Combinatória Epitopos/imunologia Imunidade Celular/imunologia Peptídeos/imunologia [Mh] Termos MeSH secundário: Adjuvantes Imunológicos/farmacologia Sequência de Aminoácidos Animais Apresentação do Antígeno/efeitos dos fármacos Apresentação do Antígeno/imunologia Células Apresentadoras de Antígenos/imunologia Proliferação Celular/efeitos dos fármacos Células Clonais Apresentação Cruzada/efeitos dos fármacos Apresentação Cruzada/imunologia Epitopos/química Epitopos/efeitos dos fármacos Imunidade Celular/efeitos dos fármacos Ativação Linfocitária/efeitos dos fármacos Ativação Linfocitária/imunologia Camundongos Dados de Sequência Molecular Ovalbumina/imunologia Peptídeos/química Poli I/farmacologia Polissacarídeos/farmacologia Complexo de Endopeptidases do Proteassoma/metabolismo Sequências Repetitivas de Aminoácidos Receptores Depuradores Classe A/metabolismo Linfócitos T Citotóxicos/efeitos dos fármacos Linfócitos T Citotóxicos/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Adjuvants, Immunologic); 0 (Epitopes); 0 (Peptides); 0 (Polysaccharides); 0 (Scavenger Receptors, Class A); 25249-22-3 (Poly I); 9006-59-1 (Ovalbumin); 9072-19-9 (fucoidan); EC 3.4.25.1 (Proteasome Endopeptidase Complex) [Em] Mês de entrada: 1506 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 141025 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0110425

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[PMID]: 25280235 [Au] Autor: Sorokin VA; Valeev VA; Usenko EL; Andrushchenko VV [Ad] Endereço: B.I. Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine , 47 Lenin Avenue, Kharkov, 61103, Ukraine. [Ti] Título: Metallization of single-stranded polyI by Zn(2+) ions in neutral solutions. [So] Source: J Phys Chem B;118(43):12360-5, 2014 Oct 30. [Is] ISSN: 1520-5207 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Metallization of single-stranded polyinosinic acid (polyI) by Zn(2+) ions at pH 7.0 was studied by differential UV spectroscopy at different temperatures. It was found that polyI is metallized at N7 and N1 atoms of hypoxanthine. The concentration dependence of the degree of binding of Zn(2+) ions to both N7 and N1 sites was obtained, and the corresponding binding constants were determined. Metallization of N1 occurs due to Zn(2+) substituting the imino protons and is effective not only at alkaline but also at neutral pH. This makes multistranded polyI-based systems more promising candidates for use in nanoelectronics than natural DNA sequences, metallization of which can be achieved only at alkaline pH. [Mh] Termos MeSH primário: Poli I/química Zinco/química [Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio Polimerização Prótons Soluções Espectrofotometria Ultravioleta Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Protons); 0 (Solutions); 25249-22-3 (Poly I); J41CSQ7QDS (Zinc) [Em] Mês de entrada: 1510 [Cu] Atualização por classe: 141030 [Lr] Data última revisão: 141030 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 141004 [St] Status: MEDLINE [do] DOI: 10.1021/jp5065903

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[PMID]: 25057007 [Au] Autor: Epaulard O; Adam L; Poux C; Zurawski G; Salabert N; Rosenbaum P; Dereuddre-Bosquet N; Zurawski S; Flamar AL; Oh S; Romain G; Chapon C; Banchereau J; Lévy Y; Le Grand R; Martinon F [Ad] Endereço: French Alternative Energies and Atomic Energy Commission, Division of Immuno-Virology, Institute for Emerging Diseases and Innovative Therapies, Infectious Diseases Models for Innovative Therapies Center, 92265 Fontenay-aux-Roses, France; Unité Mixte de Recherche E1, Université Paris-Sud, 91405 Orsa [Ti] Título: Macrophage- and neutrophil-derived TNF-α instructs skin langerhans cells to prime antiviral immune responses. [So] Source: J Immunol;193(5):2416-26, 2014 Sep 01. [Is] ISSN: 1550-6606 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Dendritic cells are major APCs that can efficiently prime immune responses. However, the roles of skin-resident Langerhans cells (LCs) in eliciting immune responses have not been fully understood. In this study, we demonstrate for the first time, to our knowledge, that LCs in cynomolgus macaque skin are capable of inducing antiviral-specific immune responses in vivo. Targeting HIV-Gag or influenza hemagglutinin Ags to skin LCs using recombinant fusion proteins of anti-Langerin Ab and Ags resulted in the induction of the viral Ag-specific responses. We further demonstrated that such Ag-specific immune responses elicited by skin LCs were greatly enhanced by TLR ligands, polyriboinosinic polyribocytidylic acid, and R848. These enhancements were not due to the direct actions of TLR ligands on LCs, but mainly dependent on TNF-α secreted from macrophages and neutrophils recruited to local tissues. Skin LC activation and migration out of the epidermis are associated with macrophage and neutrophil infiltration into the tissues. More importantly, blocking TNF-α abrogated the activation and migration of skin LCs. This study highlights that the cross-talk between innate immune cells in local tissues is an important component for the establishment of adaptive immunity. Understanding the importance of local immune networks will help us to design new and effective vaccines against microbial pathogens. [Mh] Termos MeSH primário: HIV-1/imunologia Glicoproteínas de Hemaglutininação de Vírus da Influenza/farmacologia Vírus da Influenza A/imunologia Células de Langerhans/imunologia Pele/imunologia Fator de Necrose Tumoral alfa/imunologia Produtos do Gene gag do Vírus da Imunodeficiência Humana/farmacologia [Mh] Termos MeSH secundário: Imunidade Adaptativa/efeitos dos fármacos Imunidade Adaptativa/fisiologia Animais Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia Imidazóis/farmacologia Macaca mulatta Macrófagos/imunologia Neutrófilos/imunologia Poli I/farmacologia Produtos do Gene gag do Vírus da Imunodeficiência Humana/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (Imidazoles); 0 (Tumor Necrosis Factor-alpha); 0 (gag Gene Products, Human Immunodeficiency Virus); 25249-22-3 (Poly I); V3DMU7PVXF (resiquimod) [Em] Mês de entrada: 1412 [Cu] Atualização por classe: 161019 [Lr] Data última revisão: 161019 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 140725 [St] Status: MEDLINE [do] DOI: 10.4049/jimmunol.1303339

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[PMID]: 24792476 [Au] Autor: Basu A; Jaisankar P; Suresh Kumar G [Ad] Endereço: Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India; Biophysical Chemistry Laboratory, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India. [Ti] Título: Interaction of 9-O-N-aryl/arylalkyl amino carbonyl methyl berberine analogs with single stranded ribonucleotides. [So] Source: J Photochem Photobiol B;134:64-74, 2014 May 05. [Is] ISSN: 1873-2682 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Studies on the molecular aspects of alkaloid-RNA complexation are of prime importance for the development of rational RNA targeted drug design strategies. Towards this goal, the binding aspects of three novel 9-O-N-aryl/arylalkyl amino carbonyl methyl substituted berberine analogs to four single stranded ribonucleotides, poly(G), poly(I), poly(C) and poly(U), were studied for the first time employing multifaceted biophysical tools. Absorbance and fluorescence studies revealed that these analogs bound non-cooperatively to poly(G) and poly(I) with binding affinities remarkably higher than berberine. The binding of these analogs to poly(U) and poly(C) was weaker in comparison to poly(G) and poly(I) but were one order higher in comparison to berberine. Quantum efficiency values revealed that energy transfer occurred from the RNA bases to the analogs upon complexation. The binding was dominated by large positive entropic contributions and small but favorable enthalpic contributions. Salt dependent studies established that the binding was dominated by hydrophobic forces that contributed around 90% of the total standard molar Gibbs energy. The chain length of the substitution at the 9-position was found to be critical in modulating the binding affinities. These results provide new insights into the binding efficacy of these novel berberine analogs to single stranded RNA sequences. [Mh] Termos MeSH primário: Berberina/análogos & derivados Ribonucleotídeos/química [Mh] Termos MeSH secundário: Berberina/metabolismo Sítios de Ligação Calorimetria Dicroísmo Circular Conformação de Ácido Nucleico Concentração Osmolar Poli C/química Poli C/metabolismo Poli G/química Poli G/metabolismo Poli I/química Poli I/metabolismo Poli U/química Poli U/metabolismo Teoria Quântica RNA/química RNA/metabolismo Ribonucleotídeos/metabolismo Espectrometria de Fluorescência Temperatura Ambiente Termodinâmica [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Ribonucleotides); 0I8Y3P32UF (Berberine); 25191-14-4 (Poly G); 25249-22-3 (Poly I); 27416-86-0 (Poly U); 30811-80-4 (Poly C); 63231-63-0 (RNA) [Em] Mês de entrada: 1501 [Cu] Atualização por classe: 140520 [Lr] Data última revisão: 140520 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140506 [St] Status: MEDLINE

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[PMID]: 24528536 [Au] Autor: Wynant N; Santos D; Van Wielendaele P; Vanden Broeck J [Ad] Endereço: Molecular Developmental Physiology and Signal Transduction, Department of Animal Physiology and Neurobiology, KU Leuven, Leuven, Belgium. [Ti] Título: Scavenger receptor-mediated endocytosis facilitates RNA interference in the desert locust, Schistocerca gregaria. [So] Source: Insect Mol Biol;23(3):320-9, 2014 Jun. [Is] ISSN: 1365-2583 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: RNA interference (RNAi) has become a widely used loss-of-function tool in eukaryotes; however, the delivery of double-stranded (ds)RNA) to the target cells remains a major challenge when exploiting the RNAi-technology. In insects, the efficiency of RNAi is highly species-dependent. Yet, the mechanism of cell entry in insects has only been characterized in a cell line of the fruit fly, Drosophila melanogaster, a species that is well known to be poorly amenable to environmental RNAi. In the present paper, we demonstrate that silencing vacuolar H-ATPase 16 (vha16) and clathrin heavy chain (clath), two components of the Clathrin-dependent endocytosis pathway, together with pharmacological inhibition of scavenger receptors with polyinosine and dextran sulphate, can significantly attenuate the highly robust RNAi response in the desert locust, Schistocerca gregaria. [Mh] Termos MeSH primário: Gafanhotos/genética Interferência de RNA/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Clatrina Sulfato de Dextrana Endocitose Poli I RNA de Cadeia Dupla/metabolismo Receptores Depuradores [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Clathrin); 0 (RNA, Double-Stranded); 0 (Receptors, Scavenger); 25249-22-3 (Poly I); 9042-14-2 (Dextran Sulfate) [Em] Mês de entrada: 1412 [Cu] Atualização por classe: 140505 [Lr] Data última revisão: 140505 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140218 [St] Status: MEDLINE [do] DOI: 10.1111/imb.12083

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[PMID]: 24010701 [Au] Autor: van Dijk R; Montenegro-Miranda PS; Riviere C; Schilderink R; ten Bloemendaal L; van Gorp J; Duijst S; de Waart DR; Beuers U; Haisma HJ; Bosma PJ [Ad] Endereço: Department of Gastroenterology & Hepatology and Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, 1105 BK Amsterdam, The Netherlands. r.vandijk@amc.uva.nl [Ti] Título: Polyinosinic acid blocks adeno-associated virus macrophage endocytosis in vitro and enhances adeno-associated virus liver-directed gene therapy in vivo. [So] Source: Hum Gene Ther;24(9):807-13, 2013 Sep. [Is] ISSN: 1557-7422 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Adeno-associated virus serotype 8 (AAV8) has been demonstrated to be effective for liver-directed gene therapy in humans. Although hepatocytes are the main target cell for AAV8, there is a loss of the viral vector because of uptake by macrophages and Kupffer cells. Reducing this loss would increase the efficacy of viral gene therapy and allow a dose reduction. The receptor mediating this uptake has not been identified; a potential candidate seems the macrophage scavenger receptor A (SR-A) that is involved in the endocytosis of, for instance, adenovirus. In this study we show that SR-A can mediate scAAV8 endocytosis and that blocking it with polyinosinic acid (poly[i]) reduces endocytosis significantly in vitro. Subsequently, we demonstrate that blocking this receptor improves scAAV-mediated liver-directed gene therapy in a model for inherited hyperbilirubinemia, the uridine diphospho-glucuronyl transferase 1A1-deficient Gunn rat. In male rats, preadministration of poly[i] increases the efficacy of a low dose (1×10¹¹ gc/kg) but not of a higher dose (3×10¹¹ gc/kg) scAAV8-LP1-UT1A1. Administration of poly[i] just before the vector significantly increases the correction of serum bilirubin in female rats. In these, the effect of poly[i] is seen by both doses but is more pronounced in the females receiving the low vector, where it also results in a significant increase of bilirubin glucuronides in bile. In conclusion, this study shows that SR-A mediates the endocytosis of AAV8 in vitro and in vivo and that blocking this receptor can improve the efficacy of AAV-mediated liver-directed gene therapy. [Mh] Termos MeSH primário: Dependovirus/imunologia Endocitose/efeitos dos fármacos Macrófagos do Fígado/imunologia Poli I/metabolismo Receptores Depuradores Classe A/antagonistas & inibidores [Mh] Termos MeSH secundário: Animais Bilirrubina/sangue Células CHO Linhagem Celular Cricetulus Síndrome de Crigler-Najjar/genética Síndrome de Crigler-Najjar/terapia Modelos Animais de Doenças Feminino Terapia Genética/métodos Vetores Genéticos Glucuronosiltransferase/genética Células HEK293 Hepatócitos/virologia Seres Humanos Macrófagos do Fígado/efeitos dos fármacos Fígado/imunologia Fígado/metabolismo Masculino Ratos Receptores Depuradores Classe A/efeitos dos fármacos Receptores Depuradores Classe A/metabolismo Transdução Genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Scavenger Receptors, Class A); 25249-22-3 (Poly I); EC 2.4.1.17 (Glucuronosyltransferase); RFM9X3LJ49 (Bilirubin) [Em] Mês de entrada: 1403 [Cu] Atualização por classe: 130909 [Lr] Data última revisão: 130909 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130910 [St] Status: MEDLINE [do] DOI: 10.1089/hum.2013.086

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[PMID]: 23695243 [Au] Autor: Mitkevich VA; Schulga AA; Trofimov AA; Dorovatovskii PV; Goncharuk DA; Tkach EN; Makarov AA; Polyakov KM [Ad] Endereço: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow 119991, Russian Federation. [Ti] Título: Structure and functional studies of the ribonuclease binase Glu43Ala/Phe81Ala mutant. [So] Source: Acta Crystallogr D Biol Crystallogr;69(Pt 6):991-6, 2013 Jun. [Is] ISSN: 1399-0047 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Ribonuclease from Bacillus intermedius (binase) is a small basic protein with antitumour activity. The three-dimensional structure of the binase mutant form Glu43Ala/Phe81Ala was determined at 1.98 Å resolution and its functional properties, such as the kinetic parameters characterizing the hydrolysis of polyinosinic acid and cytotoxicity towards Kasumi-1 cells, were investigated. In all crystal structures of binase studied previously the characteristic dimer is present, with the active site of one subunit being blocked owing to interactions within the dimer. In contrast to this, the new mutant form is not dimeric in the crystal. The catalytic efficiency of the mutant form is increased 1.7-fold and its cytotoxic properties are enhanced compared with the wild-type enzyme. [Mh] Termos MeSH primário: Endorribonucleases/química Endorribonucleases/genética Proteínas Mutantes/química Proteínas Mutantes/genética Poli I/metabolismo [Mh] Termos MeSH secundário: Bacillus/química Bacillus/enzimologia Bacillus/genética Catálise Linhagem Celular Tumoral Sobrevivência Celular/efeitos dos fármacos Endorribonucleases/metabolismo Seres Humanos Cinética Modelos Moleculares Mutagênese Proteínas Mutantes/metabolismo Difração de Raios X [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Mutant Proteins); 25249-22-3 (Poly I); EC 3.1.- (Endoribonucleases); EC 3.1.27.1 (ribonuclease T(2)) [Em] Mês de entrada: 1310 [Cu] Atualização por classe: 130522 [Lr] Data última revisão: 130522 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130523 [St] Status: MEDLINE [do] DOI: 10.1107/S0907444913004046

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[PMID]: 23187479 [Au] Autor: Chen YD; Fuh AY; Cheng KT [Ad] Endereço: Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan. [Ti] Título: Optically and thermally controllable light scattering based on dye-doped liquid crystals in poly(N-vinylcarbazole) films-coated liquid crystal cell. [So] Source: Opt Express;20(24):26252-60, 2012 Nov 19. [Is] ISSN: 1094-4087 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This paper presents the optically controllable light scattering based on dye-doped liquid crystals (DDLCs) in a cell, whose substrates are coated with poly(N-vinylcarbazole) (PVK) films. The optical control mechanism is the light-induced dissolution of PVK in DDLCs, which reforms the disordered LC distribution into multiple and micron-sized LC domains. The induced thermal effect on the process is investigated in detail. Scanning electron microscopy images are obtained to show the surface structures of the produced PVK films. The generated scattering can be switched back to the original one by particular thermally induced phase separation. Results indicate that the light-induced thermal effect and photoisomerization lead to the dissolution of PVK in DDLCs. Finally, scattering mode light shutter with different transmission is successfully achieved by illuminating the cell under various light intensities. [Mh] Termos MeSH primário: Corantes/química Cristalização/métodos Luz Cristais Líquidos/química Fotoquímica/métodos Poli I Espalhamento de Radiação [Mh] Termos MeSH secundário: Corantes/efeitos da radiação Desenho de Equipamento Glicocálix Temperatura Alta Seres Humanos Isomerismo Lasers Cristais Líquidos/efeitos da radiação [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Coloring Agents); 25249-22-3 (Poly I); 68777-95-7 (poly If) [Em] Mês de entrada: 1305 [Cu] Atualização por classe: 121128 [Lr] Data última revisão: 121128 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 121129 [St] Status: MEDLINE [do] DOI: 10.1364/OE.20.026252

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