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  1 / 2827 MEDLINE  
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[PMID]:29446290
[Au] Autor:Shayakhmetov SV; Lisetskaya LG; Meshchakova NM; Merinov AV
[Ti] Título:[Hygienic assessment of toxic dust factor at the aluminium smelter in Eastern Siberia].
[So] Source:Gig Sanit;95(12):1155-60, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:There is presented the assessment of priority toxic matters in the air of working zone for basic occupations of aluminum smelting by electrolysis. There are presented results of different production technologies. There are presented the results of the physical-chemical analysis of the morphology and composition of produced toxic-dust complexes.
[Mh] Termos MeSH primário: Poluentes Ocupacionais do Ar
Alumínio
Indústria Química
Poeira/análise
[Mh] Termos MeSH secundário: Poluentes Ocupacionais do Ar/análise
Poluentes Ocupacionais do Ar/toxicidade
Alumínio/análise
Alumínio/química
Indústria Química/métodos
Indústria Química/normas
Misturas Complexas/análise
Misturas Complexas/toxicidade
Eletrólise/métodos
Seres Humanos
Ciência dos Materiais/métodos
Exposição Ocupacional/efeitos adversos
Exposição Ocupacional/análise
Exposição Ocupacional/prevenção & controle
Saúde do Trabalhador
Sibéria/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants, Occupational); 0 (Complex Mixtures); 0 (Dust); CPD4NFA903 (Aluminum)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE


  2 / 2827 MEDLINE  
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[PMID]:28470141
[Au] Autor:Leslie LJ; Vasanthi Bathrinarayanan P; Jackson P; Mabiala Ma Muanda JA; Pallett R; Stillman CJP; Marshall LJ
[Ad] Endereço:a School of Engineering and Applied Science , Aston University , Birmingham , UK.
[Ti] Título:A comparative study of electronic cigarette vapor extracts on airway-related cell lines in vitro.
[So] Source:Inhal Toxicol;29(3):126-136, 2017 02.
[Is] ISSN:1091-7691
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The use of electronic cigarettes (ECs) is rapidly increasing worldwide; however, scientific evidence regarding EC cytotoxicity is limited. The aim of this study was to evaluate the acute cytotoxicity of EC vapor extract (ECE) on airway-related cells in vitro. Cigarette smoke extract (CSE), vapor extract of fifteen brands/flavors of ECs and the extract from the E-vehicle (propylene glycol and glycerin) was collected. Extracts, in concentrations of 100-12.5%, were added to human bronchial epithelial (BEAS-2B, IB3-1 and C38), fibroblast (Wi-38) and macrophage (J774 and THP-1) cell lines. Viability was assessed after 24 h using a standard XTT assay. Viability of <70% of control (no extract) was considered cytotoxic according to UNI EN ISO 10993-5 standards. CSE displayed a concentration-dependent influence on cell viability across all four cell lines with 100% producing the most toxic effect, therefore validating the model and indicating higher cytotoxicity than in ECEs. ECEs did reduce viability although this was not correlated with nicotine content or the E-vehicle. However, several flavors proved cytotoxic, with variation between different brands and cell lines. These data indicate that not all ECs are the same and that use of a particular flavor or brand may have differing effects. The cell line used is also an important factor. More research is crucial to ascertain the health effects of different ECs before they can be accepted as a safe alternative to tobacco cigarettes.
[Mh] Termos MeSH primário: Misturas Complexas/toxicidade
Sistemas Eletrônicos de Liberação de Nicotina
Aromatizantes/toxicidade
Fumaça
[Mh] Termos MeSH secundário: Brônquios/citologia
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Macrófagos/efeitos dos fármacos
Nicotina/toxicidade
Tabaco
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (Flavoring Agents); 0 (Smoke); 6M3C89ZY6R (Nicotine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1080/08958378.2017.1318193


  3 / 2827 MEDLINE  
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[PMID]:28471110
[Au] Autor:Luo Y; Li WL; Huang WH; Liu XH; Song YG; Qu HB
[Ad] Endereço:Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
[Ti] Título:Rapid quantification of multi-components in alcohol precipitation liquid of Codonopsis Radix using near infrared spectroscopy (NIRS).
[So] Source:J Zhejiang Univ Sci B;18(5):383-392, 2017 May.
[Is] ISSN:1862-1783
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:A near infrared spectroscopy (NIRS) approach was established for quality control of the alcohol precipitation liquid in the manufacture of Codonopsis Radix. By applying NIRS with multivariate analysis, it was possible to build variation into the calibration sample set, and the Plackett-Burman design, Box-Behnken design, and a concentrating-diluting method were used to obtain the sample set covered with sufficient fluctuation of process parameters and extended concentration information. NIR data were calibrated to predict the four quality indicators using partial least squares regression (PLSR). In the four calibration models, the root mean squares errors of prediction (RMSEPs) were 1.22 µg/ml, 10.5 µg/ml, 1.43 µg/ml, and 0.433% for lobetyolin, total flavonoids, pigments, and total solid contents, respectively. The results indicated that multi-components quantification of the alcohol precipitation liquid of Codonopsis Radix could be achieved with an NIRS-based method, which offers a useful tool for real-time release testing (RTRT) of intermediates in the manufacture of Codonopsis Radix.
[Mh] Termos MeSH primário: Codonopsis/química
Medicamentos de Ervas Chinesas/química
Etanol/química
Precipitação Fracionada/métodos
Modelos Estatísticos
Extratos Vegetais/química
Espectroscopia de Luz Próxima ao Infravermelho/métodos
[Mh] Termos MeSH secundário: Misturas Complexas/análise
Misturas Complexas/química
Simulação por Computador
Medicamentos de Ervas Chinesas/análise
Modelos Químicos
Extratos Vegetais/análise
Soluções/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts); 0 (Solutions); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1631/jzus.B1600141


  4 / 2827 MEDLINE  
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[PMID]:29072706
[Au] Autor:Schopper S; Kahraman A; Leuenberger P; Feng Y; Piazza I; Müller O; Boersema PJ; Picotti P
[Ad] Endereço:Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
[Ti] Título:Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry.
[So] Source:Nat Protoc;12(11):2391-2410, 2017 Nov.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Protein structural changes induced by external perturbations or internal cues can profoundly influence protein activity and thus modulate cellular physiology. A number of biophysical approaches are available to probe protein structural changes, but these are not applicable to a whole proteome in a biological extract. Limited proteolysis-coupled mass spectrometry (LiP-MS) is a recently developed proteomics approach that enables the identification of protein structural changes directly in their complex biological context on a proteome-wide scale. After perturbations of interest, proteome extracts are subjected to a double-protease digestion step with a nonspecific protease applied under native conditions, followed by complete digestion with the sequence-specific protease trypsin under denaturing conditions. This sequential treatment generates structure-specific peptides amenable to bottom-up MS analysis. Next, a proteomics workflow involving shotgun or targeted MS and label-free quantification is applied to measure structure-dependent proteolytic patterns directly in the proteome extract. Possible applications of LiP-MS include discovery of perturbation-induced protein structural alterations, identification of drug targets, detection of disease-associated protein structural states, and analysis of protein aggregates directly in biological samples. The approach also enables identification of the specific protein regions involved in the structural transition or affected by the binding event. Sample preparation takes approximately 2 d, followed by one to several days of MS and data analysis time, depending on the number of samples analyzed. Scientists with basic biochemistry training can implement the sample preparation steps. MS measurement and data analysis require a background in proteomics.
[Mh] Termos MeSH primário: Proteólise
Proteoma/análise
Proteômica/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Biomarcadores/análise
Misturas Complexas/química
Desenho de Drogas
Endopeptidase K/química
Ficina/química
Células HeLa
Seres Humanos
Pronase/química
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Proteoma/química
Proteômica/instrumentação
Controle de Qualidade
Saccharomyces cerevisiae/química
Saccharomyces cerevisiae/metabolismo
Termolisina/química
Tripsina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Complex Mixtures); 0 (Proteome); EC 3.4.21.4 (Trypsin); EC 3.4.21.64 (Endopeptidase K); EC 3.4.22.3 (Ficain); EC 3.4.24.- (Pronase); EC 3.4.24.27 (Thermolysin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.100


  5 / 2827 MEDLINE  
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[PMID]:28982779
[Au] Autor:Parisis N; Krasinska L; Harker B; Urbach S; Rossignol M; Camasses A; Dewar J; Morin N; Fisher D
[Ad] Endereço:IGMM, CNRS Univ. Montpellier, Montpellier, France.
[Ti] Título:Initiation of DNA replication requires actin dynamics and formin activity.
[So] Source:EMBO J;36(21):3212-3231, 2017 Nov 02.
[Is] ISSN:1460-2075
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nuclear actin regulates transcriptional programmes in a manner dependent on its levels and polymerisation state. This dynamics is determined by the balance of nucleocytoplasmic shuttling, formin- and redox-dependent filament polymerisation. Here, using egg extracts and human somatic cells, we show that actin dynamics and formins are essential for DNA replication. In proliferating cells, formin inhibition abolishes nuclear transport and initiation of DNA replication, as well as general transcription. In replicating nuclei from transcriptionally silent egg extracts, we identified numerous actin regulators, and disruption of actin dynamics abrogates nuclear transport, preventing NLS (nuclear localisation signal)-cargo release from RanGTP-importin complexes. Nuclear formin activity is further required to promote loading of cyclin-dependent kinase (CDK) and proliferating cell nuclear antigen (PCNA) onto chromatin, as well as initiation and elongation of DNA replication. Therefore, actin dynamics and formins control DNA replication by multiple direct and indirect mechanisms.
[Mh] Termos MeSH primário: Actinas/genética
Cromatina/metabolismo
Replicação do DNA
Proteínas Fetais/genética
Proteínas dos Microfilamentos/genética
Proteínas Nucleares/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Actinas/metabolismo
Transporte Ativo do Núcleo Celular/genética
Animais
Linhagem Celular Tumoral
Núcleo Celular/metabolismo
Cromatina/química
Misturas Complexas/química
Citoplasma/metabolismo
Células Epiteliais/citologia
Células Epiteliais/metabolismo
Proteínas Fetais/metabolismo
Regulação da Expressão Gênica
Células HeLa
Seres Humanos
Carioferinas/genética
Carioferinas/metabolismo
Proteínas dos Microfilamentos/metabolismo
Sinais de Localização Nuclear
Proteínas Nucleares/metabolismo
Antígeno Nuclear de Célula em Proliferação/genética
Antígeno Nuclear de Célula em Proliferação/metabolismo
Transdução de Sinais
Xenopus laevis
Zigoto/química
Proteína ran de Ligação ao GTP/genética
Proteína ran de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Chromatin); 0 (Complex Mixtures); 0 (Fetal Proteins); 0 (Karyopherins); 0 (Microfilament Proteins); 0 (Nuclear Localization Signals); 0 (Nuclear Proteins); 0 (Proliferating Cell Nuclear Antigen); 147336-47-8 (formin 1); EC 3.6.5.2 (ran GTP-Binding Protein)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.15252/embj.201796585


  6 / 2827 MEDLINE  
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[PMID]:28957362
[Au] Autor:Ito T; Suzaki K
[Ad] Endereço:Division of Grape and Persimmon Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Higashihiroshima, Hiroshima, Japan.
[Ti] Título:Universal detection of phytoplasmas and Xylella spp. by TaqMan singleplex and multiplex real-time PCR with dual priming oligonucleotides.
[So] Source:PLoS One;12(9):e0185427, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phytoplasmas and Xylella spp. are bacteria that cause many economically important plant diseases worldwide. TaqMan probe-based quantitative real-time polymerase chain reaction (qPCR) assays have been utilized to universally detect phytoplasmas or Xylella fastidiosa. To develop a superior universal qPCR method, we used a dual priming oligonucleotide (DPO) with two annealing sites as a reverse primer to target the well-conserved bacterial 16S rDNA. The new qPCR assays universally detected various species of phytoplasmas and subspecies of X. fastidiosa as well as Xylella taiwanensis, and generally showed superior threshold cycle values when amplifying specific or non-specific products compared to current universal qPCR assays. The proposed qPCR assays were integrated to develop a multiplex qPCR assay that simultaneously detected phytoplasmas, Xylella spp., and an internal plant DNA positive control within 1 hour. This assay could detect a minimum of ten bacterial cells and was compatible with crude extractions used in the rapid screening of various plants. The amplicons were of sufficient lengths to be directly sequenced for preliminary identification, and the primers could be used in universal conventional PCR assays. Additionally, reverse DPO primers can be utilized to improve other probe-based qPCR assays.
[Mh] Termos MeSH primário: Primers do DNA/genética
Reação em Cadeia da Polimerase Multiplex/métodos
Phytoplasma/genética
Phytoplasma/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Xylella/genética
Xylella/isolamento & purificação
[Mh] Termos MeSH secundário: Misturas Complexas
Sondas de Oligonucleotídeos/genética
Vitis/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (DNA Primers); 0 (Oligonucleotide Probes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185427


  7 / 2827 MEDLINE  
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[PMID]:28807548
[Au] Autor:Muhammad N; Subhani Q; Wang F; Guo D; Zhao Q; Wu S; Zhu Y
[Ad] Endereço:Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
[Ti] Título:Application of a simple column-switching ion chromatography technique for removal of matrix interferences and sensitive fluorescence determination of acidic compounds (pharmaceutical drugs) in complex samples.
[So] Source:J Chromatogr A;1515:69-80, 2017 Sep 15.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This work illustrates the introduction of a simple, rugged and flexible column-switching ion chromatography (IC) technique for an automated on-line QuEChERS extracted samples extracts washing followed by sensitive fluorescence (FLD) determination of five acidic pharmaceutical drugs namely; clofibric acid (CLO), ibuprofen (IBU), aspirin (ASP), naproxen (NAP) and flurobrofen (FLU) in three complex samples (spinach, apple and hospital sewage sludge). An old anion exchange column IonPac AS11-HC was utilized as a pre-treatment column for on-line washing of inorganic and organic interferences followed by isocratic separation of five acidic drugs with another anion exchange IonPac AS12A analytical column by exploiting the column-switching technique. This novel method exhibited good linearity with correlation coefficients (r ) for all drugs were in the range 0.976-0.996. The limit of detection and quantification of all five acidic drugs were in the range 0.024µg/kg to 8.70µg/kg and 0.082µg/kg to 0.029mg/kg, respectively, and better recoveries in the range 81.17-112.5% with percentage relative standard deviations (RSDs) less than 17.8% were obtained. This on-line sample pre-treatment method showed minimum matrix effect in the range of 0.87-1.25 except for aspirin. This simple rugged and flexible column-switching system required only 28min for maximum elimination of matrices and interferences in three complex samples extracts, isocratic separation of five acidic drugs and for the continuous regeneration of pre-treatment column prior to every subsequent analysis. Finally, this simple automated IC system was appeared so rugged and flexible, which can eliminate and wash out most of interference, impurities and matrices in complex samples, simply by adjusting the NaOH and acetonitrile concentration in washing mobile phase with maximum recoveries of acidic analytes of interest.
[Mh] Termos MeSH primário: Ácidos/análise
Técnicas de Química Analítica/métodos
Cromatografia por Troca Iônica
Preparações Farmacêuticas/análise
[Mh] Termos MeSH secundário: Misturas Complexas/química
Fluorescência
Limite de Detecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids); 0 (Complex Mixtures); 0 (Pharmaceutical Preparations)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  8 / 2827 MEDLINE  
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[PMID]:28774622
[Au] Autor:Parvez S; Rice GE; Teuschler LK; Simmons JE; Speth TF; Richardson SD; Miltner RJ; Hunter ES; Pressman JG; Strader LF; Klinefelter GR; Goldman JM; Narotsky MG
[Ad] Endereço:Indiana University Richard M. Fairbanks School of Public Health, Department of Environmental Health Sciences, IUPUI Campus, Indianapolis, IN 46202, USA.
[Ti] Título:Method to assess component contribution to toxicity of complex mixtures: Assessment of puberty acquisition in rats exposed to disinfection byproducts.
[So] Source:J Environ Sci (China);58:311-321, 2017 Aug.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A method based on regression modeling was developed to discern the contribution of component chemicals to the toxicity of highly complex, environmentally realistic mixtures of disinfection byproducts (DBPs). Chemical disinfection of drinking water forms DBP mixtures. Because of concerns about possible reproductive and developmental toxicity, a whole mixture (WM) of DBPs produced by chlorination of a water concentrate was administered as drinking water to Sprague-Dawley (S-D) rats in a multigenerational study. Age of puberty acquisition, i.e., preputial separation (PPS) and vaginal opening (VO), was examined in male and female offspring, respectively. When compared to controls, a slight, but statistically significant delay in puberty acquisition was observed in females but not in males. WM-induced differences in the age at puberty acquisition were compared to those reported in S-D rats administered either a defined mixture (DM) of nine regulated DBPs or individual DBPs. Regression models were developed using individual animal data on age at PPS or VO from the DM study. Puberty acquisition data reported in the WM and individual DBP studies were then compared with the DM models. The delay in puberty acquisition observed in the WM-treated female rats could not be distinguished from delays predicted by the DM regression model, suggesting that the nine regulated DBPs in the DM might account for much of the delay observed in the WM. This method is applicable to mixtures of other types of chemicals and other endpoints.
[Mh] Termos MeSH primário: Desinfetantes/toxicidade
Maturidade Sexual/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Misturas Complexas/toxicidade
Desinfecção
Feminino
Masculino
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (Disinfectants); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE


  9 / 2827 MEDLINE  
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[PMID]:28723799
[Au] Autor:Zhao GS; Liu Y; Zhang Q; Li C; Zhang YW; Ren ZZ; Zhou J; Zhang M
[Ad] Endereço:aDepartment of Interventional Therapy, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning bDepartment of Radiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi cCentral Laboratory, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning dDepartment of Hepatobiliary Intervention, Beijing Tsinghua Changgung Hospital, Changping, Beijing, China.
[Ti] Título:Transarterial chemoembolization combined with Huaier granule for the treatment of primary hepatic carcinoma: Safety and efficacy.
[So] Source:Medicine (Baltimore);96(29):e7589, 2017 Jul.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To evaluate the safety and efficacy of transarterial arterial chemoembolization (TACE) with gelatin sponge particles (GSPs-TACE) and Huaier granule to treat primary hepatic carcinoma (PHC).A series of 62 patients with PHC were included between June 2009 and December 2011, and randomly assigned to a control (n = 31) or an experimental group (n = 31). The control patients received TACE with 350 to 560 µm GSPs plus lobaplatin chemotherapy. Patients in the experimental group received TACE plus Huaier granule. Treatment safety and mid-to-long-term efficacy were evaluated.Follow-up ranged from 12 to 24 months with a mean of 28.7 months. The 6- and 12-month overall survivals were 100% and 93.5% in the experimental group and 90.3% and 80.6% in control group, respectively. The difference in overall survival at 12 months was significant (χ = 5.213, P < .05), but the difference in median survival in the experimental group (20.6 months) and control group (17.1 months) patients was not significant (χ = 0.745, P > .05). The number of TACE procedures in the experimental group (2.9 ±â€Š8.7) and control group (4.1 ±â€Š7.3) patients was significantly different (χ = 7.262, P < .05). The 6-month (87.1% vs. 73.3%, χ = 5.945) and 12-month (72.4% vs. 64.3%, χ = 6.384) tumor objective response rates in the experimental and control groups were significantly different (P < .05). There were no statistically significant differences in the occurrence of treatment-related adverse reactions in the 2 groups.Transarterial chemoembolization with GSPs and Huaier granule was safe and effective for treating PHC patients.
[Mh] Termos MeSH primário: Antineoplásicos/uso terapêutico
Carcinoma Hepatocelular/terapia
Quimioembolização Terapêutica
Misturas Complexas/uso terapêutico
Medicamentos de Ervas Chinesas/uso terapêutico
[Mh] Termos MeSH secundário: Idoso
Antineoplásicos/efeitos adversos
Carcinoma Hepatocelular/patologia
Quimioembolização Terapêutica/efeitos adversos
Terapia Combinada/efeitos adversos
Ciclobutanos/uso terapêutico
Medicamentos de Ervas Chinesas/efeitos adversos
Feminino
Seguimentos
Seres Humanos
Estimativa de Kaplan-Meier
Masculino
Meia-Idade
Gradação de Tumores
Compostos Organoplatínicos/uso terapêutico
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Complex Mixtures); 0 (Cyclobutanes); 0 (Drugs, Chinese Herbal); 0 (Organoplatinum Compounds); 0 (huaier); OX5XK1JD8C (lobaplatin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007589


  10 / 2827 MEDLINE  
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[PMID]:28715462
[Au] Autor:Itoh Y; Voskuhl RR
[Ad] Endereço:Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, California, United States of America.
[Ti] Título:Cell specificity dictates similarities in gene expression in multiple sclerosis, Parkinson's disease, and Alzheimer's disease.
[So] Source:PLoS One;12(7):e0181349, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drug repurposing is an efficient approach in new treatment development since it leverages previous work from one disease to another. While multiple sclerosis (MS), Parkinson's disease (PD), and Alzheimer's disease (AD) are all neurodegenerative diseases of the central nervous system (CNS) and differ in many clinical and pathological aspects, it is possible that they may share some mechanistic features. We hypothesized that focusing on gene expression in a CNS cell type specific manner might uncover similarities between diseases that could be missed using whole tissue gene expression analyses. We found similarities and differences in gene expression in these three distinct diseases, depending upon cell type. Microglia genes were increased in all three diseases, and gene expression levels were correlated strongly among these three neurodegenerative diseases. In astrocytes and endothelia, upregulation and correlations were observed only between MS and PD, but not AD. Neuronal genes were down-regulated in all three diseases, but correlations of changes of individual genes between diseases were not strong. Oligodendrocyte showed gene expression changes that were not shared among the three diseases. Together these data suggest that treatments targeting microglia are most amenable to drug repurposing in MS, PD, and AD, while treatments targeting other CNS cells must be tailored to each disease.
[Mh] Termos MeSH primário: Doença de Alzheimer/metabolismo
Misturas Complexas/metabolismo
Expressão Gênica/fisiologia
Esclerose Múltipla/metabolismo
Doença de Parkinson/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Astrócitos/metabolismo
Endotélio/metabolismo
Feminino
Seres Humanos
Masculino
Análise em Microsséries
Microglia/metabolismo
Meia-Idade
Neurônios/metabolismo
Oligodendroglia/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (Frondanol-A5P)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181349



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