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Pesquisa : D20.215.226 [Categoria DeCS]
Referências encontradas : 293 [refinar]
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[PMID]:29458687
[Au] Autor:Tracz DM; Tober AD; Antonation KS; Corbett CR
[Ad] Endereço:1​National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, R3E 3R2, Canada.
[Ti] Título:MALDI-TOF mass spectrometry and high-consequence bacteria: safety and stability of biothreat bacterial sample testing in clinical diagnostic laboratories.
[So] Source:J Med Microbiol;67(3):341-346, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We considered the application of MALDI-TOF mass spectrometry for BSL-3 bacterial diagnostics, with a focus on the biosafety of live-culture direct-colony testing and the stability of stored extracts. Biosafety level 2 (BSL-2) bacterial species were used as surrogates for BSL-3 high-consequence pathogens in all live-culture MALDI-TOF experiments. Viable BSL-2 bacteria were isolated from MALDI-TOF mass spectrometry target plates after 'direct-colony' and 'on-plate' extraction testing, suggesting that the matrix chemicals alone cannot be considered sufficient to inactivate bacterial culture and spores in all samples. Sampling of the instrument interior after direct-colony analysis did not recover viable organisms, suggesting that any potential risks to the laboratory technician are associated with preparation of the MALDI-TOF target plate before or after testing. Secondly, a long-term stability study (3 years) of stored MALDI-TOF extracts showed that match scores can decrease below the threshold for reliable species identification (<1.7), which has implications for proficiency test panel item storage and distribution.
[Mh] Termos MeSH primário: Infecções Bacterianas/diagnóstico
Técnicas Bacteriológicas
Armas Biológicas
Técnicas de Laboratório Clínico/métodos
Contenção de Riscos Biológicos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Bactérias/isolamento & purificação
Infecções Bacterianas/microbiologia
Técnicas Bacteriológicas/instrumentação
Técnicas de Laboratório Clínico/instrumentação
Seres Humanos
Manejo de Espécimes/efeitos adversos
Manejo de Espécimes/instrumentação
Manejo de Espécimes/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Warfare Agents)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000695


  2 / 293 MEDLINE  
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[PMID]:28493905
[Au] Autor:Perumal Samy R; Stiles BG; Sethi G; Lim LHK
[Ad] Endereço:Department of Physiology, NUS Immunology Programme, Centre for Life Sciences, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore.
[Ti] Título:Melioidosis: Clinical impact and public health threat in the tropics.
[So] Source:PLoS Negl Trop Dis;11(5):e0004738, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This review briefly summarizes the geographical distribution and clinical impact of melioidosis, especially in the tropics. Burkholderia pseudomallei (a gram-negative bacterium) is the major causative agent for melioidosis, which is prevalent in Singapore, Malaysia, Thailand, Vietnam, and Northern Australia. Melioidosis patients are increasingly being recognized in other parts of the world. The bacteria are intrinsically resistant to many antimicrobial agents, but prolonged treatment, especially with combinations of antibiotics, may be effective. Despite therapy, the overall case fatality rate of septicemia in melioidosis remains significantly high. Intracellular survival of the bacteria within macrophages may progress to chronic infections, and about 10% of patients suffer relapses. In the coming decades, melioidosis will increasingly afflict travelers throughout many global regions. Clinicians managing travelers returning from the subtropics or tropics with severe pneumonia or septicemia should consider acute melioidosis as a differential diagnosis. Patients with open skin wounds, diabetes, or chronic renal disease are at higher risk for melioidosis and should avoid direct contact with soil and standing water in endemic regions. Furthermore, there are fears that B. pseudomallei may be used as a biological weapon. Technological advancements in molecular diagnostics and antibiotic therapy are improving the disease outcomes in endemic areas throughout Asia. Research and development efforts on vaccine candidates against melioidosis are ongoing.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Burkholderia pseudomallei/patogenicidade
Farmacorresistência Bacteriana
Melioidose/epidemiologia
[Mh] Termos MeSH secundário: Animais
Austrália/epidemiologia
Vacinas Bacterianas/uso terapêutico
Armas Biológicas
Doença Crônica
Seres Humanos
Malásia/epidemiologia
Melioidose/diagnóstico
Melioidose/prevenção & controle
Camundongos
Saúde Pública
Singapura/epidemiologia
Microbiologia do Solo
Tailândia/epidemiologia
Vietnã/epidemiologia
Virulência
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Vaccines); 0 (Biological Warfare Agents)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0004738


  3 / 293 MEDLINE  
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[PMID]:28388218
[Au] Autor:Roos J; Chue C; DiEuliis D; Emanuel P
[Ti] Título:The Department of Defense Chemical and Biological Defense Program: An Enabler of the Third Offset Strategy.
[So] Source:Health Secur;15(2):207-214, 2017 Mar/Apr.
[Is] ISSN:2326-5108
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The US Department of Defense (DOD) established programs to defend against chemical and biological weapons 100 years ago because military leaders understood that the operational capability of the US military is diminished when service member health is compromised. These threats to operational readiness can be from an overt attack using chemical and biological threats but may also arise from natural exposures. In the current era of rapidly emerging technologies, adversaries are not only rediscovering chemical and biological weapons; they are also displaying an increased propensity to employ them to cause strategic instability among deployed forces or nations undergoing conflict. The United States's investments in its Chemical and Biological Defense Program (CBDP) can be a critical enabler of the third offset strategy, which is a DOD initiative that seeks to maximize force capability to offset emerging threats. To realize this vision, the CBDP must make fundamental changes in acquiring and employing effective technologies so that enemy use of chemical and biological agents against US assets is no longer a viable option. Maximization of US force health status will provide a strategic advantage over theater opponents more vulnerable to operational degradation from chemical and biological threats.
[Mh] Termos MeSH primário: Armas Biológicas
Defesa Civil/métodos
Planejamento Estratégico
United States Department of Defense
[Mh] Termos MeSH secundário: Seres Humanos
Invenções/utilização
Ciência Militar/métodos
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Warfare Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1089/hs.2017.0008


  4 / 293 MEDLINE  
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[PMID]:28361721
[Au] Autor:Takala T
[Ti] Título:Finding Hope in Synthetic Biology.
[So] Source:Camb Q Healthc Ethics;26(2):239-245, 2017 Apr.
[Is] ISSN:1469-2147
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For some, synthetic biology represents great hope in offering possible solutions to many of the world's biggest problems, from hunger to sustainable development. Others remain fearful of the harmful uses, such as bioweapons, that synthetic biology can lend itself to, and most hold that issues of biosafety are of utmost importance. In this article, I will evaluate these points of view and conclude that although the biggest promises of synthetic biology are unlikely to become reality, and the probability of accidents is fairly substantial, synthetic biology could still be seen to benefit humanity by enhancing our ethical understanding and by offering a boost to world economy.
[Mh] Termos MeSH primário: Esperança
Segurança
Biologia Sintética/ética
[Mh] Termos MeSH secundário: Temas Bioéticos
Armas Biológicas
Teoria Ética
Seres Humanos
Princípios Morais
Biologia Sintética/economia
Biologia Sintética/tendências
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Warfare Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:E; IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1017/S0963180116000839


  5 / 293 MEDLINE  
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[PMID]:28108323
[Au] Autor:Tracz DM; Tyler AD; Cunningham I; Antonation KS; Corbett CR
[Ad] Endereço:National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
[Ti] Título:Custom database development and biomarker discovery methods for MALDI-TOF mass spectrometry-based identification of high-consequence bacterial pathogens.
[So] Source:J Microbiol Methods;134:54-57, 2017 Mar.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A high-quality custom database of MALDI-TOF mass spectral profiles was developed with the goal of improving clinical diagnostic identification of high-consequence bacterial pathogens. A biomarker discovery method is presented for identifying and evaluating MALDI-TOF MS spectra to potentially differentiate biothreat bacteria from less-pathogenic near-neighbour species.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Bactérias/patogenicidade
Técnicas de Tipagem Bacteriana
Biomarcadores/análise
Bases de Dados de Ácidos Nucleicos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Bacillus/genética
Bacillus/isolamento & purificação
Bactérias/genética
Infecções Bacterianas/diagnóstico
Infecções Bacterianas/microbiologia
Proteínas de Bactérias/isolamento & purificação
Armas Biológicas
Brucella/genética
Brucella/isolamento & purificação
Seres Humanos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/normas
Yersinia/genética
Yersinia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Biological Warfare Agents); 0 (Biomarkers)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170122
[St] Status:MEDLINE


  6 / 293 MEDLINE  
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[PMID]:28092448
[Au] Autor:Gronvall GK
[Ti] Título:Prevention of the Development or Use of Biological Weapons.
[So] Source:Health Secur;15(1):36-37, 2017 Jan/Feb.
[Is] ISSN:2326-5108
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Armas Biológicas
Guerra Biológica/prevenção & controle
Bioterrorismo/prevenção & controle
Cooperação Internacional
[Mh] Termos MeSH secundário: Biovigilância/métodos
Seres Humanos
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Warfare Agents)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE
[do] DOI:10.1089/hs.2016.0096


  7 / 293 MEDLINE  
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[PMID]:28019002
[Au] Autor:Bennett VM; McNevin D; Roffey P; Gahan ME
[Ad] Endereço:National Centre for Forensic Studies, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Bruce, ACT, 2617, Australia.
[Ti] Título:Characterization of Yersinia species by protein profiling using automated microfluidic capillary electrophoresis.
[So] Source:Forensic Sci Med Pathol;13(1):10-19, 2017 Mar.
[Is] ISSN:1556-2891
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Yersinia pestis is a biological agent of high risk to national security due to its ability to be easily disseminated and transmitted among humans. If Y. pestis was to be utilized in a deliberate disease outbreak it would be essential to rapidly and accurately identify the agent. Current identification methods for Yersinia species are limited by their reliance on cultivation, the time taken to achieve results and/or the use of protocols that are not amenable for field use. Faster identification methods are urgently required. Microfluidic capillary electrophoresis was used to identify seven Yersinia species based on their protein profiles. Further objectives included determining if Yersinia species could be detected in mixtures of milk products and Escherichia coli, determining if Yersinia could be detected in a blinded identification and reproducibility across two platforms. Two characteristic protein bands were detected at 50 kilodaltons (kDa) and between 50 and 75 kDa for the Yersinia species. Individual Yersinia species could be differentiated from one another and distinguished from E. coli, Bacillus anthracis Sterne strain and Dipel (containing Bacillus thuringiensis). Due to the high protein content of milk products Yersinia could not be detected when mixed with these but was detected when mixed with E. coli. Species were correctly identified with 96% success in blinded procedures using 12 individuals. Whilst protein profile patterns were reproducible across platforms there was some discrepancy in protein sizing. This study demonstrates that protein profiling using microfluidic capillary electrophoresis is able to rapidly and reproducibly identify and characterize Yersinia species. Results show this technique is a powerful front-line, rapid and broad range screening method capable of identifying and differentiating biological agents, hoax agents and environmental bacterial species.
[Mh] Termos MeSH primário: Proteínas de Bactérias/isolamento & purificação
Eletroforese Capilar/métodos
Yersinia/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bacillus/isolamento & purificação
Armas Biológicas
Escherichia coli/isolamento & purificação
Seres Humanos
Microfluídica
Leite/microbiologia
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Biological Warfare Agents)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161227
[St] Status:MEDLINE
[do] DOI:10.1007/s12024-016-9824-7


  8 / 293 MEDLINE  
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[PMID]:27832504
[Au] Autor:Sijbrandij T; Ligtenberg AJ; Nazmi K; Veerman EC; Bolscher JG; Bikker FJ
[Ad] Endereço:Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, 1081 LA, Amsterdam, The Netherlands.
[Ti] Título:Effects of lactoferrin derived peptides on simulants of biological warfare agents.
[So] Source:World J Microbiol Biotechnol;33(1):3, 2017 Jan.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Lactoferrin (LF) is an important immune protein in neutrophils and secretory fluids of mammals. Bovine LF (bLF) harbours two antimicrobial stretches, lactoferricin and lactoferampin, situated in close proximity in the N1 domain. To mimic these antimicrobial domain parts a chimeric peptide (LFchimera) has been constructed comprising parts of both stretches (LFcin17-30 and LFampin265-284). To investigate the potency of this construct to combat a set of Gram positive and Gram negative bacteria which are regarded as simulants for biological warfare agents, the effect on bacterial killing, membrane permeability and membrane polarity were determined in comparison to the constituent peptides and the native bLF. Furthermore we aimed to increase the antimicrobial potency of the bLF derived peptides by cationic amino acid substitutions. Overall, the bactericidal activity of the peptides could be related to membrane disturbing effects, i.e. membrane permeabilization and depolarization. Those effects were most prominent for the LFchimera. Arginine residues were found to be crucial for displaying antimicrobial activity, as lysine to arginine substitutions resulted in an increased antimicrobial activity, affecting mostly LFampin265-284 whereas arginine to lysine substitutions resulted in a decreased bactericidal activity, predominantly in case of LFcin17-30.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Lactoferrina/síntese química
Lactoferrina/farmacologia
Fragmentos de Peptídeos/síntese química
Fragmentos de Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Animais
Armas Biológicas
Bovinos
Membrana Celular/efeitos dos fármacos
Permeabilidade da Membrana Celular/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Lactoferrina/química
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biological Warfare Agents); 0 (Peptide Fragments); 0 (lactoferrampin); 146897-68-9 (lactoferricin B); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE


  9 / 293 MEDLINE  
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[PMID]:27038091
[Au] Autor:Fan Y; Moon JJ
[Ad] Endereço:Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, USA.
[Ti] Título:Particulate delivery systems for vaccination against bioterrorism agents and emerging infectious pathogens.
[So] Source:Wiley Interdiscip Rev Nanomed Nanobiotechnol;9(1), 2017 Jan.
[Is] ISSN:1939-0041
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bioterrorism agents that can be easily transmitted with high mortality rates and cause debilitating diseases pose major threats to national security and public health. The recent Ebola virus outbreak in West Africa and ongoing Zika virus outbreak in Brazil, now spreading throughout Latin America, are case examples of emerging infectious pathogens that have incited widespread fear and economic and social disruption on a global scale. Prophylactic vaccines would provide effective countermeasures against infectious pathogens and biological warfare agents. However, traditional approaches relying on attenuated or inactivated vaccines have been hampered by their unacceptable levels of reactogenicity and safety issues, whereas subunit antigen-based vaccines suffer from suboptimal immunogenicity and efficacy. In contrast, particulate vaccine delivery systems offer key advantages, including efficient and stable delivery of subunit antigens, co-delivery of adjuvant molecules to bolster immune responses, low reactogenicity due to the use of biocompatible biomaterials, and robust efficiency to elicit humoral and cellular immunity in systemic and mucosal tissues. Thus, vaccine nanoparticles and microparticles are promising platforms for clinical development of biodefense vaccines. In this review, we summarize the current status of research efforts to develop particulate vaccine delivery systems against bioterrorism agents and emerging infectious pathogens. WIREs Nanomed Nanobiotechnol 2017, 9:e1403. doi: 10.1002/wnan.1403 For further resources related to this article, please visit the WIREs website.
[Mh] Termos MeSH primário: Armas Biológicas
Doenças Transmissíveis Emergentes/prevenção & controle
Nanomedicina
Nanopartículas
Vacinas
[Mh] Termos MeSH secundário: Animais
Pesquisa Biomédica
Seres Humanos
Camundongos
Vacinas/química
Vacinas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biological Warfare Agents); 0 (Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160403
[St] Status:MEDLINE
[do] DOI:10.1002/wnan.1403


  10 / 293 MEDLINE  
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[PMID]:27599259
[Au] Autor:Singh VK; Garcia M; Wise SY; Seed TM
[Ad] Endereço:a Department of Pharmacology and Molecular Therapeutics , F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences , Bethesda , MD , USA.
[Ti] Título:Medical countermeasures for unwanted CBRN exposures: Part I chemical and biological threats with review of recent countermeasure patents.
[So] Source:Expert Opin Ther Pat;26(12):1431-1447, 2016 Dec.
[Is] ISSN:1744-7674
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The threat of chemical, biological, radiological, and nuclear (CBRN) warfare has been addressed as the uppermost risk to national security since the terrorist attacks on 11 September 2001. Despite significant scientific advances over the past several decades toward the development of safe, non-toxic and effective countermeasures to combat CBRN threats, relatively few countermeasures have been approved by the US Food and Drug Administration (US FDA). Therefore, countermeasures capable of protecting the population from the effects of CBRN attack remain a significant unmet medical need. Chemical and biological (CB) threat agents can be particularly hazardous due to their effectiveness in small quantities and ease of distribution. Area covered: This article reviews the development of countermeasures for CB threats and highlights specific threats for which at least one countermeasure has been approved following the FDA Animal Rule. Patents of CB countermeasures since 2010 have been included. Expert opinion: Nine CB countermeasures have received FDA approval for use in humans following the Animal Rule, and a number of promising CB countermeasures are currently under development. In the next few years, we should expect to have multiple countermeasures approved by the FDA for each indication allowing for more flexible and effective treatment options.
[Mh] Termos MeSH primário: Planejamento em Desastres/métodos
Desenho de Drogas
Terrorismo
[Mh] Termos MeSH secundário: Animais
Armas Biológicas
Substâncias para a Guerra Química/toxicidade
Seres Humanos
Patentes como Assunto
Estados Unidos
United States Food and Drug Administration
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biological Warfare Agents); 0 (Chemical Warfare Agents)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE



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