Base de dados : MEDLINE
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[PMID]:28863363
[Au] Autor:Turker G; Akyol Ç; Ince O; Aydin S; Ince B
[Ad] Endereço:Institute of Environmental Sciences, Bogaziçi University, Bebek, 34342 Istanbul, Turkey.
[Ti] Título:Operating conditions influence microbial community structures, elimination of the antibiotic resistance genes and metabolites during anaerobic digestion of cow manure in the presence of oxytetracycline.
[So] Source:Ecotoxicol Environ Saf;147:349-356, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The way that antibiotic residues in manure follow is one of the greatest concerns due to its potential negative impacts on microbial communities, the release of metabolites and antibiotic resistant genes (ARGs) into the nature and the loss of energy recovery in anaerobic digestion (AD) systems. This study evaluated the link between different operating conditions, the biodegradation of oxytetracycline (OTC) and the formation of its metabolites and ARGs in anaerobic digesters treating cow manure. Microbial communities and ARGs were determined through the use of quantitative real-time PCR. The biodegradation of OTC and occurrence of metabolites were determined using UV-HPLC and LC/MS/MS respectively. The maximum quantity of resistance genes was also examined at the beginning of AD tests and concentration was in the order of: tetM >tetO. The numbers of ARGs were always higher at high volatile solids (VS) content and high mixing rate. The results of the investigation revealed that relationship between mixing rate and VS content plays a crucial role for elimination of ARGs, OTC and metabolites. This can be attributed to high abundance of microorganisms due to high VS content and their increased contact with elevated mixing rate. An increased interaction between microorganisms triggers the promotion of ARGs.
[Mh] Termos MeSH primário: Antibacterianos/toxicidade
Reatores Biológicos/microbiologia
Resistência Microbiana a Medicamentos/genética
Esterco/microbiologia
Consórcios Microbianos/efeitos dos fármacos
Oxitetraciclina/toxicidade
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Antibacterianos/metabolismo
Biodegradação Ambiental
Bovinos
Cromatografia Líquida de Alta Pressão
Feminino
Esterco/análise
Consórcios Microbianos/genética
Oxitetraciclina/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure); X20I9EN955 (Oxytetracycline)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE


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[PMID]:28958128
[Au] Autor:Wang M; Liu P; Xiong W; Zhou Q; Wangxiao J; Zeng Z; Sun Y
[Ad] Endereço:National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
[Ti] Título:Fate of potential indicator antimicrobial resistance genes (ARGs) and bacterial community diversity in simulated manure-soil microcosms.
[So] Source:Ecotoxicol Environ Saf;147:817-823, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the fate of nine potential indicator antimicrobial resistance genes (ARGs) (sul1, sul2, tetB, tetM, ermB, ermF, fexA, cfr, intI1) and the diversity of bacterial communities in response to poultry manure applications to arable soil over a 90 day period. Quantitative real time PCR and Illumina high-throughput sequencing of 16S rDNA gene were used to quantify and trace ARG fate. The levels of all genes dramatically decreased over time and intI1, sul1, sul2 and tetM always had the greatest abundance and lowest dissipation rates. This indicated that more effort should be focused on the ARG elimination from manure rather than waiting for subsequent attenuation in the environment. Our sequencing results documented dramatic changes in the microbial community structure and diversity during these experiments. In poultry manure groups, Bacteroidetes and Actinobacteria were the two dominant phyla while Acidobacteria dominated the control groups. Moreover, the relative abundance of genera Corynebacterium, Pseudomonas, Ochrobactrum, Actinomadura and Bacillus, which contained potential opportunistic pathogens, changed over time suggesting that poultry manure not only strongly influenced bacterial community composition, but also selected specific bacterial communities. This study provides a glimpse of ARG fates and bacterial community diversity in soil after the application of poultry manure.
[Mh] Termos MeSH primário: Resistência Microbiana a Medicamentos/genética
Esterco/microbiologia
Consórcios Microbianos/genética
Microbiologia do Solo/normas
[Mh] Termos MeSH secundário: Genes Bacterianos
Esterco/análise
Modelos Biológicos
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase em Tempo Real
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure); 0 (RNA, Ribosomal, 16S); 0 (Soil)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE


  3 / 5092 MEDLINE  
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[PMID]:28942279
[Au] Autor:Wen X; Wang Y; Zou Y; Ma B; Wu Y
[Ad] Endereço:College of Animal Science, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
[Ti] Título:No evidential correlation between veterinary antibiotic degradation ability and resistance genes in microorganisms during the biodegradation of doxycycline.
[So] Source:Ecotoxicol Environ Saf;147:759-766, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Biodegradation of antibiotic residues in the environment by microorganisms may lead to the generation of antibiotic resistance genes (ARGs), which are of great concern to human health. The aim of this study was to determine whether there is a relationship between the ability to degrade antibiotic doxycycline (DOX) and the development of resistance genes in microorganisms. We isolated and identified ten bacterial strains from a vegetable field that had received long-term manure application as fertilizer and were capable of surviving in a series of DOX concentrations (25, 50, 80, and 100mg/L). Our results showed no evidential correlation between DOX degradation ability and the development of resistance genes among the isolated microorganisms that had high DOX degradation capability (P > 0.05). This was based on the fact that Escherichia sp. and Candida sp. were the most efficient bacterial strains to degrade DOX (92.52% and 91.63%, respectively), but their tetracycline resistance genes showed a relatively low risk of antibiotic resistance in a 7-day experiment. Moreover, the tetM of the ribosomal protection protein genes carried by these two preponderant bacteria was five-fold higher than that carried by other isolates (P < 0.05). Pearson correlations between the C /C of DOX and tet resistance genes of three isolates, except for Escherichia sp. and Candida sp., showed remarkable negative correlations (P < 0.05), mainly because tetG markedly increased during the DOX degradation process. Our results concluded that the biodegradation of antibiotic residues may not necessarily lead to the development of ARGs in the environment. In addition, the two bacteria that we isolated, namely, Escherichia sp. and Candida sp., are potential candidates for the engineering of environmentally friendly bacteria.
[Mh] Termos MeSH primário: Doxiciclina/toxicidade
Resistência Microbiana a Medicamentos/efeitos dos fármacos
Microbiologia do Solo/normas
Poluentes do Solo/toxicidade
Drogas Veterinárias/toxicidade
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Candida/efeitos dos fármacos
Candida/genética
China
Relação Dose-Resposta a Droga
Resistência Microbiana a Medicamentos/genética
Escherichia/efeitos dos fármacos
Escherichia/genética
Fertilizantes
Genes Bacterianos
Esterco/microbiologia
Resistência a Tetraciclina/efeitos dos fármacos
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Manure); 0 (Soil Pollutants); 0 (Veterinary Drugs); N12000U13O (Doxycycline)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


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[PMID]:28926818
[Au] Autor:Duan M; Gu J; Wang X; Li Y; Zhang S; Yin Y; Zhang R
[Ad] Endereço:College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Effects of genetically modified cotton stalks on antibiotic resistance genes, intI1, and intI2 during pig manure composting.
[So] Source:Ecotoxicol Environ Saf;147:637-642, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Genetically modified (GM) cotton production generates a large yield of stalks and their disposal is difficult. In order to study the feasibility of using GM cotton stalks for composting and the changes that occur in antibiotic resistance genes (ARGs) during composting, we supplemented pig manure with GM or non-GM cotton stalks during composting and we compared their effects on the absolute abundances (AA) of intI1, intI2, and ARGs under the two treatments. The compost was mature after processing based on the germination index and C/N ratio. After composting, the AAs of ARGs, intI1, and intI2 were reduced by 41.7% and 45.0% in the non-GM and GM treatments, respectively. The ARG profiles were affected significantly by temperature and ammonia nitrogen. In addition, excluding tetC, GM cotton stalks had no significant effects on ARGs, intI1, and intI2 compared with the non-GM treatment (p < 0.05). Thus, similar to non-GM cotton stalks, GM cotton stalks can be used for aerobic composting with livestock manure, and the AAs of ARGs can be reduced. Furthermore, the results of this study provide a theoretical basis for the harmless utilization of GM cotton stalks.
[Mh] Termos MeSH primário: Compostagem/métodos
Resistência Microbiana a Medicamentos/genética
Genes Bacterianos
Gossypium/genética
Esterco/análise
Plantas Geneticamente Modificadas/genética
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Sus scrofa
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  5 / 5092 MEDLINE  
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[PMID]:29251850
[Au] Autor:Daur I
[Ti] Título:Study of commercial effective microorganism on composting and dynamics of plant essential metal micronutrients.
[So] Source:J Environ Biol;37(5):937-41, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The present study addresses the problem of organic farmers' that needs local organic resources with their enhanced quality to effectively fertilize their agriculture crops. In accordance with the objective of the experiment that is about enhancing quality of compost, a blend of organic resources, comprising cow manure (CM), poultry manure (PM) and kitchen waste (KW) (2:1:1 ratio by volume) was composted with effective microorganisms (EM.1) (CompostEM.1) and without (Compostplain). During composting, temperature, pH, carbon, nitrogen, C/N ratio, total and diethylene triamine pentaacetic acid (DTPA)-extractable essential metal micronutrient (Fe3+, Cu2+, Zn2+, and Mn2+) contents of both the composts were recorded following the standard procedures. Low temperature range (24−24), low pH (6.7−7.2) and higher N-content (1.15−1.40) were recorded for CompostEM.1 as compared to Compostplain. Carbon degradation was also faster in CompostEM.1 than in Compostplain. Consequently, C/N ratio stabilization took 6 weeks in CompostEM.1 as compared to 18 weeks in Compostplain, leading to rapid completion of composting. Total concentration of micronutrients increased while their DTPA-extractable content decreased during the composting. Total micronutrient concentration was augmented more in Compostplain samples than in CompostEM.1. However, decrease in DTPA-extractable content was similar in both the composts. Increase in micronutrient content was attributed to decrease in organic matter weight, whereas decrease in metal micronutrients was attributed to the formation of organic matter-metal complexes during decomposition. Findings of the study indicated that effective micro-organisms enhanced composting process, however, further studies are required to evaluate its quality, especially effect on plant and soil.
[Mh] Termos MeSH primário: Compostagem
Lactobacillus casei/fisiologia
Metais/metabolismo
Plantas
Rodopseudomonas/fisiologia
Saccharomyces cerevisiae/fisiologia
[Mh] Termos MeSH secundário: Animais
Bactérias
Esterco
Metais/química
Resíduos Sólidos
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure); 0 (Metals); 0 (Solid Waste)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  6 / 5092 MEDLINE  
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[PMID]:29251469
[Au] Autor:Kaur R; Joshi N; Virk JS; Sharma S
[Ti] Título:Evaluation of Pseudomonas fluorescens for the management of tomato early blight disease and fruit borer.
[So] Source:J Environ Biol;37(5):869-872, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Early blight disease and fruit damage by Helicoverpa armigera are serious problems of tomato causing heavy losses in the yield. An experiment was conducted to evaluate the efficacy of talc based formulation of antagonist Pseudomonas flourescens (Psf) delivered through two different forms of substrate, farmyard manure (FYM) and vermicompost, for the management of tomato early blight disease. Two treatments of Psf, were selected Solarization of nursery soil + seeds and seedlings root dip treatment with Psf formulation + Use of FYM colonized with Psf formulation; Solarization of nursery soil + seed and seedlings root dip treatment with Psf formulation + Use of vermicompost colonized with Psf formulation. These were compared with farmer's practice and untreated control. The impact of all these treatments on tomato fruit borer, H. armigera was also studied. There was not much variation in plant growth parameters (plant height and canopy width) between all the treatments. Both biocontrol treatments (17.69% and 141.3 q acre-1; 15.70% and 139.4 q acre-1, respectively), as well as farmer's practice (10.25% and 208.5 q acre-1) were found to be better than untreated control (29.21% and 94.6 q acre-1) in reducing the incidence of early blight disease in tomato and increasing the marketable yield of tomato fruits. However, the farmer's practice was found to be significantly better than both biocontrol treatments of Psf in controlling fruit damage due to disease, as well as fruit borer. The effect of both bioagents enriched formulations i.e. FYM+Psf and vermicompost+Psf with respect to disease was significantly at par with each other, whereas they had no impact on the fruit borer incidence. The results indicated that either of biocontrol treatments of P. flourescens using farmyard manure and vermicompost, as delivery substrates, can be considered as one of the component along with chemical control in developing IPM programme for the management of early blight disease in tomato.
[Mh] Termos MeSH primário: Antibiose
Lycopersicon esculentum/microbiologia
Mariposas/microbiologia
Controle Biológico de Vetores
Doenças das Plantas/microbiologia
Pseudomonas fluorescens/fisiologia
[Mh] Termos MeSH secundário: Animais
Compostagem
Esterco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  7 / 5092 MEDLINE  
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[PMID]:29304047
[Au] Autor:Pandey P; Chiu C; Miao M; Wang Y; Settles M; Del Rio NS; Castillo A; Souza A; Pereira R; Jeannotte R
[Ad] Endereço:Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California, United States of America.
[Ti] Título:16S rRNA analysis of diversity of manure microbial community in dairy farm environment.
[So] Source:PLoS One;13(1):e0190126, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dairy farms generate a considerable amount of manure, which is applied in cropland as fertilizer. While the use of manure as fertilizer reduces the application of chemical fertilizers, the main concern with regards to manure application is microbial pollution. Manure is a reservoir of a broad range of microbial populations, including pathogens, which have potential to cause contamination and pose risks to public and animal health. Despite the widespread use of manure fertilizer, the change in microbial diversity of manure under various treatment processes is still not well-understood. We hypothesize that the microbial population of animal waste changes with manure handling used in a farm environment. Consequential microbial risk caused by animal manure may depend on manure handling. In this study, a reconnaissance effort for sampling dairy manure in California Central Valley followed by 16S rRNA analysis of content and diversity was undertaken to understand the microbiome of manure after various handling processes. The microbial community analysis of manure revealed that the population in liquid manure differs from that in solid manure. For instance, the bacteria of genus Sulfuriomonas were unique in liquid samples, while the bacteria of genus Thermos were observed only in solid samples. Bacteria of genus Clostridium were present in both solid and liquid samples. The population among liquid samples was comparable, as was the population among solid samples. These findings suggest that the mode of manure application (i.e., liquid versus solid) could have a potential impact on the microbiome of cropland receiving manure as fertilizers.
[Mh] Termos MeSH primário: Indústria de Laticínios
Esterco/microbiologia
Microbiota
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Algoritmos
Animais
Bovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Manure); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190126


  8 / 5092 MEDLINE  
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[PMID]:29235979
[Au] Autor:Ueki A; Goto K; Kaku N; Ueki K
[Ad] Endereço:Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan.
[Ti] Título:Aminipila butyrica gen. nov., sp. nov., a strictly anaerobic, arginine-decomposing bacterium isolated from a methanogenic reactor of cattle waste.
[So] Source:Int J Syst Evol Microbiol;68(1):443-448, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strictly anaerobic bacterial strain (FH042 ) was isolated from a methanogenic reactor treating waste from cattle farms. Cells were stained Gram-positive, straight to gently curved rods with polar flagella. The strain was asaccharolytic. The strain fermented amino acids (l-arginine, l-lysine and l-serine) as growth substrates and produced acetate and butyrate. The optimum temperature for growth was 30 °C and the optimum pH was 6.1-6.8. Oxidase, catalase and nitrate-reducing activities were negative. Hydrogen sulfide was produced. The genomic DNA G+C content of strain FH042 was 44.7±0.2 mol%. The major cellular fatty acids were C18 : 1ω9c DMA, C17 : 2/C17 : 1ω9c (as summed feature), C16 : 0 DMA and C14 : 0. The cell-wall peptidoglycan contained meso-diaminopimelic acid as a diagnostic amino acid. The most closely related described species on the basis of 16S rRNA gene sequences was Anaerovorax odorimutans in the family XIII Incertae Sedis in the order Clostridiales of the class Clostridia with sequence similarity of 95.1 %. Based on the distinct differences in phylogenetic and phenotypic characteristics between strain FH042 and related species, Aminipila butyrica gen. nov., sp. nov. is proposed to accommodate the strain. Type strain is FH042 (=JCM 31555 =DSM 103574 ).
[Mh] Termos MeSH primário: Arginina/metabolismo
Reatores Biológicos/microbiologia
Clostridiales/classificação
Esterco/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Bactérias Anaeróbias/classificação
Técnicas de Tipagem Bacteriana
Composição de Bases
Bovinos
Clostridiales/genética
Clostridiales/isolamento & purificação
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Japão
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Manure); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid); 94ZLA3W45F (Arginine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002534


  9 / 5092 MEDLINE  
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[PMID]:29253000
[Au] Autor:Lin S; Wang S; Si Y; Yang W; Zhu S; Ni W
[Ad] Endereço:College of Environmental and Resource Sciences, Zhejiang University, Key Laboratory of Agricultural Resource and Environment of Zhejiang Province, Hangzhou, P. R. China.
[Ti] Título:Variations in eco-enzymatic stoichiometric and microbial characteristics in paddy soil as affected by long-term integrated organic-inorganic fertilization.
[So] Source:PLoS One;12(12):e0189908, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To investigate the effects of different nutrient management regimes on the soil chemical, eco-enzymatic stoichiometric and microbial characteristics, soil samples were collected from a 30-year, long-term field experiment with six plots growing rice. The results showed that as integrated fertilization increased, so did the concentrations of soil total or available nutrients and microbial biomass carbon (MBC). Our results also found enhanced soil basal respiration and cumulative carbon mineralization compared to chemical fertilization alone at the same nutrient doses. The activities of soil protease (Pro), ß-glucosidase (ßG), N-acetyl-glucosaminidase (NAG) and acid phosphatase (AP) from the integrated fertilization treatments were significantly higher than those of the treatments without organic manure, so did the activities of soil leucyl aminopeptidase (LAP) and urease (Ure) from the treatment with organic manure in addition to farmer practise fertilization (NPKM2). The stoichiometric ratios, expressed as lnßG/ln(NAG+LAP)/lnPro/lnUre/lnAP, ranged from 1:0.94:1.04:0.67:1.01 to 1:0.98:1.10:0.78:1.25, indicating that the acquisition of C, N and P changed consistently and synchronously under different nutrient management strategies. Integrated fertilization was more beneficial to the acquisition and utilization of soil organic carbon compared to low-molecular-weight organic nitrogen. We concluded that protease and urease should be considered in eco-enzymatic stoichiometric assessments for the hydrolysis of proteins, amino acids, carbohydrates and phosphomonoesters in soil, and integrated fertilization with chemical fertilizers and organic manure should be recommended as a preferable nutrient management system for intensive rice cultivation.
[Mh] Termos MeSH primário: Agricultura/métodos
Carbono/química
Fertilizantes
Microbiologia do Solo
Solo/química
[Mh] Termos MeSH secundário: Acetilglucosaminidase/metabolismo
Fosfatase Ácida/metabolismo
Aminopeptidases/metabolismo
Biomassa
China
Esterco
Nitrogênio/química
Oryza
Fósforo/química
beta-Glucosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Manure); 0 (Soil); 27YLU75U4W (Phosphorus); 7440-44-0 (Carbon); EC 3.1.3.2 (Acid Phosphatase); EC 3.2.1.21 (beta-Glucosidase); EC 3.2.1.52 (Acetylglucosaminidase); EC 3.4.11.- (Aminopeptidases); N762921K75 (Nitrogen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189908


  10 / 5092 MEDLINE  
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[PMID]:28543177
[Au] Autor:Abakpa GO; Umoh VJ; Kamaruzaman S; Ibekwe M
[Ad] Endereço:National Biotechnology Development Agency, Abuja, Nigeria.
[Ti] Título:Fingerprints of resistant Escherichia coli O157:H7 from vegetables and environmental samples.
[So] Source:J Sci Food Agric;98(1):80-86, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Some routes of transmission of Escherichia coli O157:H7 to fresh produce include contaminated irrigation water and manure polluted soils. The aim of the present study was to determine the genetic relationships of E. coli O157:H7 isolated from some produce growing region in Nigeria using enterobacterial repetitive intergenic consensus (ERIC) DNA fingerprinting analysis. A total of 440 samples comprising leafy greens, irrigation water, manure and soil were obtained from vegetable producing regions in Kano and Plateau States, Nigeria. Genes coding for the quinolone resistance-determinant (gyrA) and plasmid (pCT) coding for multidrug resistance (MDR) were determined using polymerase chain reaction (PCR) in 16 isolates that showed MDR. RESULTS: Cluster analysis of the ERIC-PCR profiles based on band sizes revealed six main clusters from the sixteen isolates analysed. The largest cluster (cluster 3) grouped isolates from vegetables and manure at a similarity coefficient of 0.72. CONCLUSION: The present study provides data that support the potential transmission of resistant strains of E. coli O157:H7 from vegetables and environmental sources to humans with potential public health implications, especially in developing countries. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Escherichia coli O157/isolamento & purificação
Contaminação de Alimentos/análise
Microbiologia do Solo
Verduras/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Irrigação Agrícola
Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Escherichia coli O157/classificação
Escherichia coli O157/efeitos dos fármacos
Escherichia coli O157/genética
Esterco/microbiologia
Nigéria
Folhas de Planta/microbiologia
Quinolonas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure); 0 (Quinolones)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8441



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